Dlx5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dlx5
Entrez Gene ID	25431
Full Name	distal-less homeobox 5
Synonyms	RDLX
General protein information	Preferred Names distal-less homeobox 5 Names homeobox protein DLX-5 DLX-3 homeobox protein DLX-3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q21
Summary	homeobox protein that is required for BMP-2-mediated oseoblast differentiation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017592486.1	XP_017447975.1	homeobox protein DLX-5 isoform X1
NM_012943.1	NP_037075.1	homeobox protein DLX-5

Pathways from BioSystems

Homologies

	DLX5	XP_001090332.1
Gallus gallus (chicken)	DLX5	NP_989490.1
Pan troglodytes (chimpanzee)	DLX5	XP_519218.2
Mus musculus (house mouse)	Dlx5	NP_034186.2
Danio rerio (zebrafish)	dlx5a	NP_571381.1
Canis lupus familiaris (dog)	DLX5	XP_850412.1
Homo sapiens (human)	DLX5	NP_005212.1
Bos taurus (cattle)	DLX5	NP_001068781.1
Rattus norvegicus (Norway rat)	Dlx5	NP_037075.1
Xenopus tropicalis (tropical clawed frog)	dlx5	NP_001004778.1

Gene Ontology
Bibliography

Related articles in PubMed

The Dlx5 and Foxg1 transcription factors, linked via miRNA-9 and -200, are required for the development of the olfactory and GnRH system.
Garaffo G, Conte D, Provero P, Tomaiuolo D, Luo Z, Pinciroli P, Peano C, D'Atri I, Gitton Y, Etzion T, Gothilf Y, Gays D, Santoro MM, Merlo GR
Molecular and cellular neurosciences68103-19(2015 Sep)

Osteogenic transcription factors and proto-oncogene regulate bone sialoprotein gene transcription.
Takai H, Mezawa M, Choe J, Nakayama Y, Ogata Y
Journal of oral science55(3)209-15(2013 Sep)

Homeodomain transcription factors regulate BMP-2-induced osteoactivin transcription in osteoblasts.
Singh M, Del Carpio-Cano FE, Monroy MA, Popoff SN, Safadi FF
Journal of cellular physiology227(1)390-9(2012 Jan)

The canonical BMP signaling pathway plays a crucial part in stimulation of dentin sialophosphoprotein expression by BMP-2.
Cho YD, Yoon WJ, Woo KM, Baek JH, Park JC, Ryoo HM
The Journal of biological chemistry285(47)36369-76(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that overexpression of Runx2, Dlx5 or c-Src stimulates BSP transcription, and suggest that Runx2, Dlx5 and c-Src might be crucial transcriptional regulators of mineralization and bone formation
Title: Osteogenic transcription factors and proto-oncogene regulate bone sialoprotein gene transcription.

OA transcription is differentially regulated by Dlx3, Dlx5, and Msx2 during osteoblast differentiation.
Title: Homeodomain transcription factors regulate BMP-2-induced osteoactivin transcription in osteoblasts.

Dlx5 specifically transactivates the Runx2 P1 promoter, and its action on the P1 promoter is antagonized by Msx2
Title: Dlx5 specifically regulates Runx2 type II expression by binding to homeodomain-response elements in the Runx2 distal promoter.

Dlx5 transactivates ALP expression, directly by binding to its cognate response element and/or indirectly by stimulating Runx2 expression, and Msx2 counteracts the direct transactivation of Dlx5
Title: Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2.

Results describe gene regulation by Dlx3 in relation to that of Msx2 and Dlx5 during osteoblast differentiation.
Title: Dlx3 transcriptional regulation of osteoblast differentiation: temporal recruitment of Msx2, Dlx3, and Dlx5 homeodomain proteins to chromatin of the osteocalcin gene.

The following Dlx5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dlx5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01396	NM_012943.1 Latest version!	Rattus norvegicus distal-less homeobox 5 (Dlx5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa60773	XM_017592486.1 Latest version!	Rattus norvegicus distal-less homeobox 5 (Dlx5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dlx5 ( NM_012943.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037075.1 Rattus norvegicus distal-less homeobox 5 (Dlx5), mRNA.

Price & Availability↑

CloneID	ORa01396
Clone ID Related Accession (Same CDS sequence)	NM_012943.1
Accession Version	NM_012943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	homeobox protein DLX-5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L24443.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L24443.1, D31734.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348, SAMN02642610 [ECO:0000348] ##Evidence-Data-END##

ATGACAGGAG TGTTTGACAG AAGAGTCCCA AGCATCCGAT CCGGCGACTT CCAAGCTCCG 
TTCCCGACGT CCGCCGCCAT GCACCACCCG TCTCAGGAAT CGCCAACTTT GCCGGAGTCC 
TCGGCCACCG ATTCTGACTA CTACAGTCCC GCGGGGGCCG CCCCTCATGG CTACTGCTCT 
CCTACCTCTG CTTCTTACCG CAAAGCGCTC AACCCATACC AGTACCAGTA TCACAGCGTG 
AACGGCTCCG CAGCCGGCTA CCCGGCCAAG GCTTATGCGG ACTACGGCTA CGCCAGCCCC 
TACCACCAGT ACGGAGGCGC CTACAACCGA GTCCCGAGTG CCACCAGCCA GCCAGAGAAA 
GAAGTGGCCG AGCCAGAGGT GAGGATGGTG AATGGTAAAC CAAAGAAAGT TCGTAAACCC 
AGGACTATTT ATTCCAGCTT TCAGCTGGCC GCTTTACAGA GAAGGTTTCA GAAGACTCAG 
TACCTCGCCC TGCCAGAACG CGCGGAGTTG GCCGCCTCTC TAGGACTGAC GCAAACACAG 
GTGAAAATCT GGTTTCAGAA CAAAAGATCC AAGATCAAGA AGATCATGAA AAACGGGGAG 
ATGCCCCCGG AGCACAGTCC CAGCTCCAGC GACCCTATGG CGTGTAACTC GCCACAGTCA 
CCAGCGGTGT GGGAGCCCCA GGGCTCATCC CGCTCTCTCA GCCACCACCC TCATGCCCAC 
CCTACGACCT CCAACCAGTC CCCCGCGTCC AGCTACCTGG AGAACTCGGC TTCCTGGTAC 
CCAAGTGCAG CCAGCTCAAT CAATTCCCAC CTGCCACCGC CTGGCTCCCT GCAGCACCCG 
CTGGCACTGG CCTCCGGGAC GCTTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037075.1
CDS	179..1048
Translation	MTGVFDRRVPSIRSGDFQAPFPTSAAMHHPSQESPTLPESSATDSDYYSPAGAAPHGYCSPTSASYRKALNPYQYQYHSVNGSAAGYPAKAYADYGYASPYHQYGGAYNRVPSATSQPEKEVAEPEVRMVNGKPKKVRKPRTIYSSFQLAALQRRFQKTQYLALPERAELAASLGLTQTQVKIWFQNKRSKIKKIMKNGEMPPEHSPSSSDPMACNSPQSPAVWEPQGSSRSLSHHPHAHPTTSNQSPASSYLENSASWYPSAASSINSHLPPPGSLQHPLALASGTLY

Target ORF information:

RefSeq Version	NM_012943.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus distal-less homeobox 5 (Dlx5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012943.1

ORF Insert Sequence:

ATGACAGGAG TGTTTGACAG AAGAGTCCCA AGCATCCGAT CCGGCGACTT CCAAGCTCCG 
TTCCCGACGT CCGCCGCCAT GCACCACCCG TCTCAGGAAT CGCCAACTTT GCCGGAGTCC 
TCGGCCACCG ATTCTGACTA CTACAGTCCC GCGGGGGCCG CCCCTCATGG CTACTGCTCT 
CCTACCTCTG CTTCTTACCG CAAAGCGCTC AACCCATACC AGTACCAGTA TCACAGCGTG 
AACGGCTCCG CAGCCGGCTA CCCGGCCAAG GCTTATGCGG ACTACGGCTA CGCCAGCCCC 
TACCACCAGT ACGGAGGCGC CTACAACCGA GTCCCGAGTG CCACCAGCCA GCCAGAGAAA 
GAAGTGGCCG AGCCAGAGGT GAGGATGGTG AATGGTAAAC CAAAGAAAGT TCGTAAACCC 
AGGACTATTT ATTCCAGCTT TCAGCTGGCC GCTTTACAGA GAAGGTTTCA GAAGACTCAG 
TACCTCGCCC TGCCAGAACG CGCGGAGTTG GCCGCCTCTC TAGGACTGAC GCAAACACAG 
GTGAAAATCT GGTTTCAGAA CAAAAGATCC AAGATCAAGA AGATCATGAA AAACGGGGAG 
ATGCCCCCGG AGCACAGTCC CAGCTCCAGC GACCCTATGG CGTGTAACTC GCCACAGTCA 
CCAGCGGTGT GGGAGCCCCA GGGCTCATCC CGCTCTCTCA GCCACCACCC TCATGCCCAC 
CCTACGACCT CCAACCAGTC CCCCGCGTCC AGCTACCTGG AGAACTCGGC TTCCTGGTAC 
CCAAGTGCAG CCAGCTCAAT CAATTCCCAC CTGCCACCGC CTGGCTCCCT GCAGCACCCG 
CTGGCACTGG CCTCCGGGAC GCTTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlx5 ( XM_017592486.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447975.1 Rattus norvegicus distal-less homeobox 5 (Dlx5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa60773
Clone ID Related Accession (Same CDS sequence)	XM_017592486.1
Accession Version	XM_017592486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	homeobox protein DLX-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGAG TGTTTGACAG AAGAGTCCCA AGCATCCGAT CCGGCGACTT CCAAGCTCCG 
TTCCCGACGT CCGCCGCCAT GCACCACCCG TCTCAGGAAT CGCCAACTTT GCCGGAGTCC 
TCGGCCACCG ATTCTGACTA CTACAGTCCC GCGGGGGCCG CCCCTCATGG CTACTGCTCT 
CCTACCTCTG CTTCTTACCG CAAAGCGCTC AACCCATACC AGTACCAGTA TCACAGCGTG 
AACGGCTCCG CAGCCGGCTA CCCGGCCAAG GCTTATGCGG ACTACGGCTA CGCCAGCCCC 
TACCACCAGT ACGGAGGCGC CTACAACCGA GTCCCGAGTG CCACCAGCCA GCCAGCTTTC 
AGCTGGCCGC TTTACAGAGA AGGTTTCAGA AGACTCAGTA CCTCGCCCTG CCAGAACGCG 
CGGAGTTGGC CGCCTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447975.1
CDS	257..697
Translation	MTGVFDRRVPSIRSGDFQAPFPTSAAMHHPSQESPTLPESSATDSDYYSPAGAAPHGYCSPTSASYRKALNPYQYQYHSVNGSAAGYPAKAYADYGYASPYHQYGGAYNRVPSATSQPAFSWPLYREGFRRLSTSPCQNARSWPPL

Target ORF information:

RefSeq Version	XM_017592486.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus distal-less homeobox 5 (Dlx5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592486.1

ORF Insert Sequence:

ATGACAGGAG TGTTTGACAG AAGAGTCCCA AGCATCCGAT CCGGCGACTT CCAAGCTCCG 
TTCCCGACGT CCGCCGCCAT GCACCACCCG TCTCAGGAAT CGCCAACTTT GCCGGAGTCC 
TCGGCCACCG ATTCTGACTA CTACAGTCCC GCGGGGGCCG CCCCTCATGG CTACTGCTCT 
CCTACCTCTG CTTCTTACCG CAAAGCGCTC AACCCATACC AGTACCAGTA TCACAGCGTG 
AACGGCTCCG CAGCCGGCTA CCCGGCCAAG GCTTATGCGG ACTACGGCTA CGCCAGCCCC 
TACCACCAGT ACGGAGGCGC CTACAACCGA GTCCCGAGTG CCACCAGCCA GCCAGCTTTC 
AGCTGGCCGC TTTACAGAGA AGGTTTCAGA AGACTCAGTA CCTCGCCCTG CCAGAACGCG 
CGGAGTTGGC CGCCTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Dlx5 and Foxg1 transcription factors, linked via miRNA-9 and -200, are required for the development of the olfactory and GnRH system.
Molecular and cellular neurosciences68103-19(2015 Sep)
Garaffo G,Conte D,Provero P,Tomaiuolo D,Luo Z,Pinciroli P,Peano C,D'Atri I,Gitton Y,Etzion T,Gothilf Y,Gays D,Santoro MM,Merlo GR

Osteogenic transcription factors and proto-oncogene regulate bone sialoprotein gene transcription.
Journal of oral science55(3)209-15(2013 Sep)
Takai H,Mezawa M,Choe J,Nakayama Y,Ogata Y

Homeodomain transcription factors regulate BMP-2-induced osteoactivin transcription in osteoblasts.
Journal of cellular physiology227(1)390-9(2012 Jan)
Singh M,Del Carpio-Cano FE,Monroy MA,Popoff SN,Safadi FF

The canonical BMP signaling pathway plays a crucial part in stimulation of dentin sialophosphoprotein expression by BMP-2.
The Journal of biological chemistry285(47)36369-76(2010 Nov)
Cho YD,Yoon WJ,Woo KM,Baek JH,Park JC,Ryoo HM