Spp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spp1
Entrez Gene ID	25353
Full Name	secreted phosphoprotein 1
Synonyms	OSP
General protein information	Preferred Names secreted phosphoprotein 1 Names osteopontin SPP-1 Sialoprotein (osteopontin) bone sialoprotein 1 osteopontin-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	sialoprotein that binds osteosarcoma cells; may play a role in osteoclast cell function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008769996.2	XP_008768218.1	osteopontin isoform X1
NM_012881.2	NP_037013.2	osteopontin precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPP1	XP_001159959.1
Canis lupus familiaris (dog)	SPP1	XP_003434072.1
Rattus norvegicus (Norway rat)	Spp1	NP_037013.2
	SPP1	XP_002804172.1
Mus musculus (house mouse)	Spp1	NP_001191130.1
Homo sapiens (human)	SPP1	NP_001035147.1
Bos taurus (cattle)	SPP1	NP_776612.1

Gene Ontology
Bibliography

Related articles in PubMed

Chromatin organization regulated by EZH2-mediated H3K27me3 is required for OPN-induced migration of bone marrow-derived mesenchymal stem cells.
Liu L, Luo Q, Sun J, Ju Y, Morita Y, Song G
The international journal of biochemistry & cell biology9629-39(2018 Jan)

Relationship of Cx43 regulation of vascular permeability to osteopontin-tight junction protein pathway after sepsis in rats.
Zhang J, Yang G, Zhu Y, Peng X, Li T, Liu L
American journal of physiology. Regulatory, integrative and comparative physiology314(1)R1-R11(2018 Jan)

Osteopontin regulates the proliferation of rat aortic smooth muscle cells in response to gingipains treatment.
Cao C, Luo X, Ji X, Wang Y, Zhang Y, Zhang P, Zhong L
Molecular and cellular probes3351-56(2017 Jun)

Implication of hyperoxaluria on osteopontin and ER stress mediated apoptosis in renal tissue of rats.
Bhardwaj R, Bhardwaj A, Tandon C, Dhawan DK, Bijarnia RK, Kaur T
Experimental and molecular pathology102(3)384-390(2017 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The extent of calcium oxalate crystal deposition is directly associated with the expression of osteopontin, inflammation and endoplasmic reticulum stress in renal tissue.
Title: Implication of hyperoxaluria on osteopontin and ER stress mediated apoptosis in renal tissue of rats.

Secreted phosphoprotein 1 is a key regulatory factor that affects nerve degeneration and regeneration through c-Fos, PKCalpha and p-ERK/ERK pathways after rat sciatic nerve injury.
Title: Effect of Spp1 on nerve degeneration and regeneration after rat sciatic nerve injury.

OPN might enhance inflammation and cell proliferation, attenuate cell apoptosis, and ultimately facilitate liver regeneration at the termination stage of liver regeneration.
Title: A preliminary in vivo study of the effects of OPN on rat liver regeneration induced by partial hepatectomy.

OPN is elevated in the aortas of diabetic rats.
Title: High glucose induces the expression of osteopontin in blood vessels in?vitro and in?vivo.

The analysis showed that the expression profiles of Spp1 signaling-related genes were notably different in four types of liver diseases: nonalcoholic fatty liver disease, liver cirrhosis, liver cancer, and acute liver failure. (Review)
Title: Comparative analysis of gene expression profiles of OPN signaling pathway in four kinds of liver diseases.

The following Spp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12527	NM_012881.2 Latest version!	Rattus norvegicus secreted phosphoprotein 1 (Spp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa12527	XM_008769996.2 Latest version!	Rattus norvegicus secreted phosphoprotein 1 (Spp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spp1 ( NM_012881.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037013.2 Rattus norvegicus secreted phosphoprotein 1 (Spp1), mRNA.

Price & Availability↑

CloneID	ORa12527
Clone ID Related Accession (Same CDS sequence)	XM_008769996.2 , NM_012881.2
Accession Version	NM_012881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	osteopontin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CV102664.1, BC078874.1 and BU759036.1. On Apr 12, 2007 this sequence version replaced NM_012881.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078874.1, FQ222192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642782 [ECO:0000348] ##Evidence-Data-END##

ATGAGACTGG CAGTGGTTTG CTTTTGCCTG TTCGGCCTTG CCTCCTGTCT CCCGGTGAAA 
GTGGCTGAGT TTGGCAGCTC AGAGGAGAAG GCGCATTACA GCAAACACTC AGATGCTGTA 
GCCACTTGGC TGAAGCCTGA CCCATCTCAG AAGCAGAATC TTCTAGCCCC ACAGAATTCT 
GTGTCCTCTG AAGAAACGGA TGACTTTAAG CAAGAAACTC TTCCAAGCAA CTCCAATGAA 
AGCCATGACC ACATGGACGA TGATGACGAC GACGATGACG ACGGAGACCA TGCAGAGAGC 
GAGGATTCTG TGAACTCGGA TGAATCTGAC GAATCTCACC ATTCCGATGA ATCTGATGAG 
TCCTTCACTG CCAGCACACA AGCAGACGTT TTGACTCCAA TCGCCCCCAC AGTCGATGTC 
CCTGACGGCC GAGGTGATAG CTTGGCTTAC GGACTGAGGT CAAAGTCCAG GAGTTTCCCT 
GTTTCTGATG AACAGTATCC CGATGCCACA GATGAGGACC TCACCTCCCG CATGAAGAGC 
CAGGAGTCCG ATGAGGCTAT CAAGGTCATC CCAGTTGCCC AGCGTCTGAG CGTGCCCTCT 
GATCAGGACA GCAACGGGAA GACCAGCCAT GAGTCAAGTC AGCTGGATGA ACCAAGCGTG 
GAAACACACA GCCTGGAGCA GTCCAAGGAG TATAAGCAGA GGGCCAGCCA CGAGAGCACT 
GAGCAGTCGG ATGCGATCGA TAGTGCCGAG AAGCCGGATG CAATCGATAG TGCGGAGCGG 
TCGGATGCTA TCGACAGTCA GGCGAGTTCC AAAGCCAGCC TGGAACATCA GAGCCACGAG 
TTTCACAGCC ATGAGGACAA GCTAGTCCTA GACCCTAAGA GTAAGGAAGA TGATAGGTAT 
CTGAAATTCC GCATTTCTCA TGAATTAGAG AGTTCATCTT CTGAGGTCAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037013.2
CDS	107..1060
Translation	MRLAVVCFCLFGLASCLPVKVAEFGSSEEKAHYSKHSDAVATWLKPDPSQKQNLLAPQNSVSSEETDDFKQETLPSNSNESHDHMDDDDDDDDDGDHAESEDSVNSDESDESHHSDESDESFTASTQADVLTPIAPTVDVPDGRGDSLAYGLRSKSRSFPVSDEQYPDATDEDLTSRMKSQESDEAIKVIPVAQRLSVPSDQDSNGKTSHESSQLDEPSVETHSLEQSKEYKQRASHESTEQSDAIDSAEKPDAIDSAERSDAIDSQASSKASLEHQSHEFHSHEDKLVLDPKSKEDDRYLKFRISHELESSSSEVN

Target ORF information:

RefSeq Version	NM_012881.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus secreted phosphoprotein 1 (Spp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012881.2

ORF Insert Sequence:

ATGAGACTGG CAGTGGTTTG CTTTTGCCTG TTCGGCCTTG CCTCCTGTCT CCCGGTGAAA 
GTGGCTGAGT TTGGCAGCTC AGAGGAGAAG GCGCATTACA GCAAACACTC AGATGCTGTA 
GCCACTTGGC TGAAGCCTGA CCCATCTCAG AAGCAGAATC TTCTAGCCCC ACAGAATTCT 
GTGTCCTCTG AAGAAACGGA TGACTTTAAG CAAGAAACTC TTCCAAGCAA CTCCAATGAA 
AGCCATGACC ACATGGACGA TGATGACGAC GACGATGACG ACGGAGACCA TGCAGAGAGC 
GAGGATTCTG TGAACTCGGA TGAATCTGAC GAATCTCACC ATTCCGATGA ATCTGATGAG 
TCCTTCACTG CCAGCACACA AGCAGACGTT TTGACTCCAA TCGCCCCCAC AGTCGATGTC 
CCTGACGGCC GAGGTGATAG CTTGGCTTAC GGACTGAGGT CAAAGTCCAG GAGTTTCCCT 
GTTTCTGATG AACAGTATCC CGATGCCACA GATGAGGACC TCACCTCCCG CATGAAGAGC 
CAGGAGTCCG ATGAGGCTAT CAAGGTCATC CCAGTTGCCC AGCGTCTGAG CGTGCCCTCT 
GATCAGGACA GCAACGGGAA GACCAGCCAT GAGTCAAGTC AGCTGGATGA ACCAAGCGTG 
GAAACACACA GCCTGGAGCA GTCCAAGGAG TATAAGCAGA GGGCCAGCCA CGAGAGCACT 
GAGCAGTCGG ATGCGATCGA TAGTGCCGAG AAGCCGGATG CAATCGATAG TGCGGAGCGG 
TCGGATGCTA TCGACAGTCA GGCGAGTTCC AAAGCCAGCC TGGAACATCA GAGCCACGAG 
TTTCACAGCC ATGAGGACAA GCTAGTCCTA GACCCTAAGA GTAAGGAAGA TGATAGGTAT 
CTGAAATTCC GCATTTCTCA TGAATTAGAG AGTTCATCTT CTGAGGTCAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spp1 ( XM_008769996.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768218.1 Rattus norvegicus secreted phosphoprotein 1 (Spp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12527
Clone ID Related Accession (Same CDS sequence)	XM_008769996.2 , NM_012881.2
Accession Version	XM_008769996.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	osteopontin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACTGG CAGTGGTTTG CTTTTGCCTG TTCGGCCTTG CCTCCTGTCT CCCGGTGAAA 
GTGGCTGAGT TTGGCAGCTC AGAGGAGAAG GCGCATTACA GCAAACACTC AGATGCTGTA 
GCCACTTGGC TGAAGCCTGA CCCATCTCAG AAGCAGAATC TTCTAGCCCC ACAGAATTCT 
GTGTCCTCTG AAGAAACGGA TGACTTTAAG CAAGAAACTC TTCCAAGCAA CTCCAATGAA 
AGCCATGACC ACATGGACGA TGATGACGAC GACGATGACG ACGGAGACCA TGCAGAGAGC 
GAGGATTCTG TGAACTCGGA TGAATCTGAC GAATCTCACC ATTCCGATGA ATCTGATGAG 
TCCTTCACTG CCAGCACACA AGCAGACGTT TTGACTCCAA TCGCCCCCAC AGTCGATGTC 
CCTGACGGCC GAGGTGATAG CTTGGCTTAC GGACTGAGGT CAAAGTCCAG GAGTTTCCCT 
GTTTCTGATG AACAGTATCC CGATGCCACA GATGAGGACC TCACCTCCCG CATGAAGAGC 
CAGGAGTCCG ATGAGGCTAT CAAGGTCATC CCAGTTGCCC AGCGTCTGAG CGTGCCCTCT 
GATCAGGACA GCAACGGGAA GACCAGCCAT GAGTCAAGTC AGCTGGATGA ACCAAGCGTG 
GAAACACACA GCCTGGAGCA GTCCAAGGAG TATAAGCAGA GGGCCAGCCA CGAGAGCACT 
GAGCAGTCGG ATGCGATCGA TAGTGCCGAG AAGCCGGATG CAATCGATAG TGCGGAGCGG 
TCGGATGCTA TCGACAGTCA GGCGAGTTCC AAAGCCAGCC TGGAACATCA GAGCCACGAG 
TTTCACAGCC ATGAGGACAA GCTAGTCCTA GACCCTAAGA GTAAGGAAGA TGATAGGTAT 
CTGAAATTCC GCATTTCTCA TGAATTAGAG AGTTCATCTT CTGAGGTCAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768218.1
CDS	203..1156
Translation	MRLAVVCFCLFGLASCLPVKVAEFGSSEEKAHYSKHSDAVATWLKPDPSQKQNLLAPQNSVSSEETDDFKQETLPSNSNESHDHMDDDDDDDDDGDHAESEDSVNSDESDESHHSDESDESFTASTQADVLTPIAPTVDVPDGRGDSLAYGLRSKSRSFPVSDEQYPDATDEDLTSRMKSQESDEAIKVIPVAQRLSVPSDQDSNGKTSHESSQLDEPSVETHSLEQSKEYKQRASHESTEQSDAIDSAEKPDAIDSAERSDAIDSQASSKASLEHQSHEFHSHEDKLVLDPKSKEDDRYLKFRISHELESSSSEVN

Target ORF information:

RefSeq Version	XM_008769996.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus secreted phosphoprotein 1 (Spp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769996.2

ORF Insert Sequence:

ATGAGACTGG CAGTGGTTTG CTTTTGCCTG TTCGGCCTTG CCTCCTGTCT CCCGGTGAAA 
GTGGCTGAGT TTGGCAGCTC AGAGGAGAAG GCGCATTACA GCAAACACTC AGATGCTGTA 
GCCACTTGGC TGAAGCCTGA CCCATCTCAG AAGCAGAATC TTCTAGCCCC ACAGAATTCT 
GTGTCCTCTG AAGAAACGGA TGACTTTAAG CAAGAAACTC TTCCAAGCAA CTCCAATGAA 
AGCCATGACC ACATGGACGA TGATGACGAC GACGATGACG ACGGAGACCA TGCAGAGAGC 
GAGGATTCTG TGAACTCGGA TGAATCTGAC GAATCTCACC ATTCCGATGA ATCTGATGAG 
TCCTTCACTG CCAGCACACA AGCAGACGTT TTGACTCCAA TCGCCCCCAC AGTCGATGTC 
CCTGACGGCC GAGGTGATAG CTTGGCTTAC GGACTGAGGT CAAAGTCCAG GAGTTTCCCT 
GTTTCTGATG AACAGTATCC CGATGCCACA GATGAGGACC TCACCTCCCG CATGAAGAGC 
CAGGAGTCCG ATGAGGCTAT CAAGGTCATC CCAGTTGCCC AGCGTCTGAG CGTGCCCTCT 
GATCAGGACA GCAACGGGAA GACCAGCCAT GAGTCAAGTC AGCTGGATGA ACCAAGCGTG 
GAAACACACA GCCTGGAGCA GTCCAAGGAG TATAAGCAGA GGGCCAGCCA CGAGAGCACT 
GAGCAGTCGG ATGCGATCGA TAGTGCCGAG AAGCCGGATG CAATCGATAG TGCGGAGCGG 
TCGGATGCTA TCGACAGTCA GGCGAGTTCC AAAGCCAGCC TGGAACATCA GAGCCACGAG 
TTTCACAGCC ATGAGGACAA GCTAGTCCTA GACCCTAAGA GTAAGGAAGA TGATAGGTAT 
CTGAAATTCC GCATTTCTCA TGAATTAGAG AGTTCATCTT CTGAGGTCAA TTAA