Npr3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npr3
Entrez Gene ID	25339
Full Name	natriuretic peptide receptor 3
Synonyms	ANP-CR,NPRC
General protein information	Preferred Names natriuretic peptide receptor 3 Names atrial natriuretic peptide receptor 3 ANP-C ANPR-C ANPRC Atrial natriuretic peptide clearence receptor 3 NPR-C atrial natriuretic peptide C-type receptor atrial natriuretic peptide clearance receptor atrial natriuretic peptide receptor type C natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q16
Summary	receptor for natriuretic peptide; may play role in hypertension and neurotransmitter efflux [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006232045.3	XP_006232107.1	atrial natriuretic peptide receptor 3 isoform X1
NM_012868.1	NP_037000.1	atrial natriuretic peptide receptor 3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NPR3	XP_526959.2
Mus musculus (house mouse)	Npr3	NP_001034270.1
Rattus norvegicus (Norway rat)	Npr3	NP_037000.1
Xenopus tropicalis (tropical clawed frog)	npr3	XP_002936727.2
Homo sapiens (human)	NPR3	NP_000899.1
Canis lupus familiaris (dog)	NPR3	XP_005619519.1
	NPR3	XP_001087354.2
Bos taurus (cattle)	NPR3	NP_776552.1
Gallus gallus (chicken)	NPR3	XP_004937228.1
Danio rerio (zebrafish)	npr3	NP_001096143.1

Gene Ontology
Bibliography

Related articles in PubMed

NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-?.
Lin D, Chai Y, Izadpanah R, Braun SE, Alt E
Cell cycle (Georgetown, Tex.)15(18)2414-9(2016 Sep)

Transient silencing of Npr3 gene expression improved the circulatory levels of atrial natriuretic peptides and attenuated ?-adrenoceptor activation- induced cardiac hypertrophic growth in experimental rats.
Venkatesan B, Tumala A, Subramanian V, Vellaichamy E
European journal of pharmacology78244-58(2016 Jul)

Natriuretic peptide receptor 3 (NPR3) is regulated by microRNA-100.
Wong LL, Wee AS, Lim JY, Ng JY, Chong JP, Liew OW, Lilyanna S, Martinez EC, Ackers-Johnson MA, Vardy LA, Armugam A, Jeyaseelan K, Ng TP, Lam CS, Foo RS, Richards AM, Chen YT
Journal of molecular and cellular cardiology8213-21(2015 May)

Paradoxical expressions of natriuretic peptide receptor-C and neutral endopeptidase account for C-type natriuretic peptide decline during the progression of experimental obstructive nephropathy.
Hu P, Zhao XQ, Wang J, Kong HB, Hu B, Lu L, Qin YH
Journal of the renin-angiotensin-aldosterone system : JRAAS15(4)458-65(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-alpha, which are regulators of apoptosis.
Title: NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-?.

down-regulation of Npr3 gene improves the circulatory levels of ANP and antioxidant system and there by attenuates the beta-adrenoceptor over-activation mediated cardiac hypertrophic growth
Title: Transient silencing of Npr3 gene expression improved the circulatory levels of atrial natriuretic peptides and attenuated ?-adrenoceptor activation- induced cardiac hypertrophic growth in experimental rats.

Results from this study point to a role for miR-100 in the regulation of NPR3 expression, and suggest a possible therapeutic target for modulation of NP bioactivity in heart disease.
Title: Natriuretic peptide receptor 3 (NPR3) is regulated by microRNA-100.

the increased expression of NPR-C (r=-0.943 and -0.837 for mRNA and protein respectively, p<0.05) and NEP (r=-0.687 and -0.823 for mRNA and protein respectively, p<0.05) were accompanied by a signi fi cant decline in CNP.
Title: Paradoxical expressions of natriuretic peptide receptor-C and neutral endopeptidase account for C-type natriuretic peptide decline during the progression of experimental obstructive nephropathy.

These results indicate that NPR-C activation by C-ANP4-23 attenuates the enhanced levels of cell cycle proteins through the inhibition of enhanced expression of Gialpha proteins
Title: Natriuretic peptide receptor-C agonist attenuates the expression of cell cycle proteins and proliferation of vascular smooth muscle cells from spontaneously hypertensive rats: role of Gi proteins and MAPkinase/PI3kinase signaling.

The following Npr3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npr3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13786	NM_012868.1 Latest version!	Rattus norvegicus natriuretic peptide receptor 3 (Npr3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa24468	XM_006232045.3 Latest version!	Rattus norvegicus natriuretic peptide receptor 3 (Npr3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npr3 ( NM_012868.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037000.1 Rattus norvegicus natriuretic peptide receptor 3 (Npr3), mRNA.

Price & Availability↑

CloneID	ORa13786
Clone ID Related Accession (Same CDS sequence)	NM_012868.1
Accession Version	NM_012868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	atrial natriuretic peptide receptor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L27339.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L27339.1, X75605.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689600 [ECO:0000348] ##Evidence-Data-END##

ATGCGGTCCC TGCTGCTGTT CACTTTCTCG GCGTGCGTGC TGCTGGCCCG GGCGCTGCTG 
GCTGGCGGTG CGAGTAGTGG CGGTGGGGAC ACCGGGCCAG GCAACAGGCG CCGGGAGAGA 
GAGGCGCTGG CGGCTCAAAA GATCGAGGTG CTTGTGCTAT TGCCCCGGGA CGATTCGTAC 
TTGTTCTCGC TGGCCCGGGT GAGGCCGGCC ATCGAGTACG CGCTGCGTAG CGTGGAGGGC 
AATGGCACCG GCAGGAAGCT GCTGCCGCCC GGCACTCGCT TCCAGGTGGC CTATGAAGAC 
TCTGACTGTG GCAACCGTGC GCTCTTCAGT TTGGTGGACC GCGTGGCGGC GGCGCGCGGC 
GCCAAGCCGG ACCTCATCCT GGGTCCGGTG TGCGAGTACG CTGCGGCGCC GGTGGCCCGG 
CTGGCGTCTC ACTGGGACCT GCCGATGCTG TCCGCAGGAG CGCTGGCCGC GGGTTTCCAG 
CACAAGGACA CGGAGTACTC GCACCTCACG CGCGTGGCGC CTGCCTACGC CAAGATGGGA 
GAGATGATGC TCGCTCTGTT CCGCCACCAC CACTGGAGCC GCGCTGCCCT GCTCTACAGC 
GACGACAAAC TCGAGAGAAA CTGCTACTTC ACTCTCGAGG GGGTCCACGA GGTTTTCCAG 
GAGGAGGGCT TGCACACGTC TGCCTACAAT TTCGACGAGA CCAAAGACTT GGACCTGGAC 
GACATAGTGC GCTACATCCA GGGCAGTGAG CGAGTGGTGA TCATGTGTGC CAGTGGTGAC 
ACCATTCGGA GAATCATGTT GGCGGTGCAC AGACACGGCA TGACCAGTGG AGACTATGCT 
TTCTTCAACA TTGAACTCTT CAACAGTTCT TCTTACGGAG ATGGCTCGTG GAAGAGAGGG 
GACAAACACG ACTTTGAAGC TAAGCAAGCT TACTCATCCC TCCAAACAGT CACCCTGCTG 
AGGACCGCGA AGCCTGAGTT TGAGAAGTTT TCCATGGAGG TGAAAAGTTC TGTTGAGAAA 
CAAGGGCTCA ATGAGGAGGA TTACGTGAAC ATGTTCGTTG AAGGCTTCCA TGATGCCATC 
CTCCTCTACG TTCTGGCTTT GCATGAAGTG CTCAGAGCTG GCTACAGCAA GAAGGATGGG 
GGGAAAATCA TCCAGCAGAC TTGGAACAGG ACATTTGAAG GTATCGCCGG GCAGGTGTCC 
ATAGATGCTA ATGGGGATCG GTATGGAGAC TTCTCTGTGG TTGCCATGAC TGATACAGAA 
GCAGGTACCC AGGAGGTCAT TGGTGATTAC TTTGGAAAAG AAGGCCGGTT CAAAATGCGA 
TCCAATGTCA AATATCCTTG GGGCTCTTTG AAATTGAGAA TAGATGAAAC CAGAATTGTG 
GAGCACACCA ACAGCTCTCC TTGCAAATCA TGTGGCCTAG AAGAATCAGC AGTGACAGGA 
ATTGTTGTGG GGGCCCTACT AGGTGCTGGT TTGCTAATGG CCTTCTACTT TTTCAGGAAG 
AAATACAGAA TAACCATTGA GAGGCGAAAT CACCAAGAAG AAAGCAACAT TGGGAAACAT 
CGAGAGCTGC GGGAAGATTC CATCAGATCA CATTTTTCGG TGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037000.1
CDS	17..1624
Translation	MRSLLLFTFSACVLLARALLAGGASSGGGDTGPGNRRREREALAAQKIEVLVLLPRDDSYLFSLARVRPAIEYALRSVEGNGTGRKLLPPGTRFQVAYEDSDCGNRALFSLVDRVAAARGAKPDLILGPVCEYAAAPVARLASHWDLPMLSAGALAAGFQHKDTEYSHLTRVAPAYAKMGEMMLALFRHHHWSRAALLYSDDKLERNCYFTLEGVHEVFQEEGLHTSAYNFDETKDLDLDDIVRYIQGSERVVIMCASGDTIRRIMLAVHRHGMTSGDYAFFNIELFNSSSYGDGSWKRGDKHDFEAKQAYSSLQTVTLLRTAKPEFEKFSMEVKSSVEKQGLNEEDYVNMFVEGFHDAILLYVLALHEVLRAGYSKKDGGKIIQQTWNRTFEGIAGQVSIDANGDRYGDFSVVAMTDTEAGTQEVIGDYFGKEGRFKMRSNVKYPWGSLKLRIDETRIVEHTNSSPCKSCGLEESAVTGIVVGALLGAGLLMAFYFFRKKYRITIERRNHQEESNIGKHRELREDSIRSHFSVA

Target ORF information:

RefSeq Version	NM_012868.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus natriuretic peptide receptor 3 (Npr3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012868.1

ORF Insert Sequence:

ATGCGGTCCC TGCTGCTGTT CACTTTCTCG GCGTGCGTGC TGCTGGCCCG GGCGCTGCTG 
GCTGGCGGTG CGAGTAGTGG CGGTGGGGAC ACCGGGCCAG GCAACAGGCG CCGGGAGAGA 
GAGGCGCTGG CGGCTCAAAA GATCGAGGTG CTTGTGCTAT TGCCCCGGGA CGATTCGTAC 
TTGTTCTCGC TGGCCCGGGT GAGGCCGGCC ATCGAGTACG CGCTGCGTAG CGTGGAGGGC 
AATGGCACCG GCAGGAAGCT GCTGCCGCCC GGCACTCGCT TCCAGGTGGC CTATGAAGAC 
TCTGACTGTG GCAACCGTGC GCTCTTCAGT TTGGTGGACC GCGTGGCGGC GGCGCGCGGC 
GCCAAGCCGG ACCTCATCCT GGGTCCGGTG TGCGAGTACG CTGCGGCGCC GGTGGCCCGG 
CTGGCGTCTC ACTGGGACCT GCCGATGCTG TCCGCAGGAG CGCTGGCCGC GGGTTTCCAG 
CACAAGGACA CGGAGTACTC GCACCTCACG CGCGTGGCGC CTGCCTACGC CAAGATGGGA 
GAGATGATGC TCGCTCTGTT CCGCCACCAC CACTGGAGCC GCGCTGCCCT GCTCTACAGC 
GACGACAAAC TCGAGAGAAA CTGCTACTTC ACTCTCGAGG GGGTCCACGA GGTTTTCCAG 
GAGGAGGGCT TGCACACGTC TGCCTACAAT TTCGACGAGA CCAAAGACTT GGACCTGGAC 
GACATAGTGC GCTACATCCA GGGCAGTGAG CGAGTGGTGA TCATGTGTGC CAGTGGTGAC 
ACCATTCGGA GAATCATGTT GGCGGTGCAC AGACACGGCA TGACCAGTGG AGACTATGCT 
TTCTTCAACA TTGAACTCTT CAACAGTTCT TCTTACGGAG ATGGCTCGTG GAAGAGAGGG 
GACAAACACG ACTTTGAAGC TAAGCAAGCT TACTCATCCC TCCAAACAGT CACCCTGCTG 
AGGACCGCGA AGCCTGAGTT TGAGAAGTTT TCCATGGAGG TGAAAAGTTC TGTTGAGAAA 
CAAGGGCTCA ATGAGGAGGA TTACGTGAAC ATGTTCGTTG AAGGCTTCCA TGATGCCATC 
CTCCTCTACG TTCTGGCTTT GCATGAAGTG CTCAGAGCTG GCTACAGCAA GAAGGATGGG 
GGGAAAATCA TCCAGCAGAC TTGGAACAGG ACATTTGAAG GTATCGCCGG GCAGGTGTCC 
ATAGATGCTA ATGGGGATCG GTATGGAGAC TTCTCTGTGG TTGCCATGAC TGATACAGAA 
GCAGGTACCC AGGAGGTCAT TGGTGATTAC TTTGGAAAAG AAGGCCGGTT CAAAATGCGA 
TCCAATGTCA AATATCCTTG GGGCTCTTTG AAATTGAGAA TAGATGAAAC CAGAATTGTG 
GAGCACACCA ACAGCTCTCC TTGCAAATCA TGTGGCCTAG AAGAATCAGC AGTGACAGGA 
ATTGTTGTGG GGGCCCTACT AGGTGCTGGT TTGCTAATGG CCTTCTACTT TTTCAGGAAG 
AAATACAGAA TAACCATTGA GAGGCGAAAT CACCAAGAAG AAAGCAACAT TGGGAAACAT 
CGAGAGCTGC GGGAAGATTC CATCAGATCA CATTTTTCGG TGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npr3 ( XM_006232045.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232107.1 Rattus norvegicus natriuretic peptide receptor 3 (Npr3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24468
Clone ID Related Accession (Same CDS sequence)	XM_006232045.3
Accession Version	XM_006232045.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	atrial natriuretic peptide receptor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAG GAAGGGAGAA TATATATATA TACATATATA TATATATATA TATATATATA 
TATATATTTA TAATAAGTCT ATATGTATAT TACAGACGCA CAGGTTTACA TTCTGTGAAC 
TTTTTTCTTT TTTCTTTCTT TGCTTTTTTT TTTTTTAAGC CTAGGGATAT TGCAGAGGAG 
TCTTCCTCTC TATCTTTTGG CGCGTTACTG AAGGGGGTAT TCTGTTTTCT TGGAGCGCCT 
AAGGGGGCGC AGGGACAGAG GAAAGAAAAG AGCAGGGAGG AAAGGGGCAG GGTGGGTGGG 
GGGCAGAGGG CGAGCCGGTG GCCAGGGCGA GCGCTTGCTG GCAACAGGAT GCGGTCCCTG 
CTGCTGTTCA CTTTCTCGGC GTGCGTGCTG CTGGCCCGGG CGCTGCTGGC TGGCGGTGCG 
AGTAGTGGCG GTGGGGACAC CGGGCCAGGC AACAGGCGCC GGGAGAGAGA GGCGCTGGCG 
GCTCAAAAGA TCGAGGTGCT TGTGCTATTG CCCCGGGACG ATTCGTACTT GTTCTCGCTG 
GCCCGGGTGA GGCCGGCCAT CGAGTACGCG CTGCGTAGCG TGGAGGGCAA TGGCACCGGC 
AGGAAGCTGC TGCCGCCCGG CACTCGCTTC CAGGTGGCCT ATGAAGACTC TGACTGTGGC 
AACCGCGCGC TCTTCAGTTT GGTGGACCGC GTGGCGGCGG CGCGCGGCGC CAAGCCGGAC 
CTCATCCTGG GTCCGGTGTG CGAGTACGCT GCGGCGCCGG TGGCCCGGCT GGCGTCTCAC 
TGGGACCTGC CGATGCTGTC CGCAGGAGCG CTGGCCGCGG GTTTCCAGCA CAAGGACACG 
GAGTACTCGC ACCTCACGCG CGTGGCGCCT GCCTACGCCA AGATGGGAGA GATGATGCTC 
GCTCTGTTCC GCCACCACCA CTGGAGCCGC GCTGCCCTGC TCTACAGCGA CGACAAACTC 
GAGAGAAACT GCTACTTCAC TCTCGAGGGG GTCCACGAGG TTTTCCAGGA GGAGGGCTTG 
CACACGTCTG CCTACAATTT CGACGAGACC AAAGACTTGG ACCTGGACGA CATAGTGCGC 
TACATCCAGG GCAGTGAGCG AGTGGTGATC ATGTGTGCCA GTGGTGACAC CATTCGGAGA 
ATCATGTTGG CGGTGCACAG ACACGGCATG ACCAGTGGAG ACTACGCTTT CTTCAACATT 
GAACTCTTCA ACAGTTCTTC TTACGGAGAT GGCTCGTGGA AGAGAGGGGA CAAACACGAC 
TTTGAAGCTA AGCAAGCTTA CTCATCCCTC CAAACAGTCA CCCTGCTGAG GACCGCGAAG 
CCTGAGTTTG AGAAGTTTTC CATGGAGGTG AAAAGTTCTG TTGAGAAACA AGGGCTCAAT 
GAGGAGGATT ACGTGAACAT GTTCGTTGAA GGCTTCCATG ATGCCATCCT CCTCTACGTT 
CTGGCTTTGC ATGAAGTGCT CAGAGCTGGC TACAGCAAGA AGGATGGGGG GAAAATCATC 
CAGCAGACTT GGAACAGGAC ATTTGAAGGT ATCGCCGGGC AGGTGTCCAT AGATGCTAAT 
GGGGATCGGT ATGGAGACTT CTCTGTGGTT GCCATGACTG ATACAGAAGC AGGTACCCAG 
GAGGTCATTG GTGATTACTT TGGAAAAGAA GGCCGGTTCA AAATGCGATC CAATGTCAAA 
TATCCTTGGG GCTCTTTGAA ATTGAGAATA GATGAAACCA GAATTGTGGA GCACACCAAC 
AGCTCTCCTT GCAAATCATC AGGTGGCCTA GAAGAATCAG CAGTGACAGG AATTGTTGTG 
GGGGCCCTAC TAGGTGCTGG TTTGCTAATG GCCTTCTACT TTTTCAGGAA GAAATACAGA 
ATAACCATTG AGAGGCGAAA TCACCAAGAA GAAAGCAACA TTGGGAAACA TCGAGAGCTG 
CGGGAAGATT CCATCAGATC ACATTTTTCG GTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232107.1
CDS	254..2212
Translation	MCKGRENIYIYIYIYIYIYIYIFIISLYVYYRRTGLHSVNFFLFSFFAFFFFKPRDIAEESSSLSFGALLKGVFCFLGAPKGAQGQRKEKSREERGRVGGGQRASRWPGRALAGNRMRSLLLFTFSACVLLARALLAGGASSGGGDTGPGNRRREREALAAQKIEVLVLLPRDDSYLFSLARVRPAIEYALRSVEGNGTGRKLLPPGTRFQVAYEDSDCGNRALFSLVDRVAAARGAKPDLILGPVCEYAAAPVARLASHWDLPMLSAGALAAGFQHKDTEYSHLTRVAPAYAKMGEMMLALFRHHHWSRAALLYSDDKLERNCYFTLEGVHEVFQEEGLHTSAYNFDETKDLDLDDIVRYIQGSERVVIMCASGDTIRRIMLAVHRHGMTSGDYAFFNIELFNSSSYGDGSWKRGDKHDFEAKQAYSSLQTVTLLRTAKPEFEKFSMEVKSSVEKQGLNEEDYVNMFVEGFHDAILLYVLALHEVLRAGYSKKDGGKIIQQTWNRTFEGIAGQVSIDANGDRYGDFSVVAMTDTEAGTQEVIGDYFGKEGRFKMRSNVKYPWGSLKLRIDETRIVEHTNSSPCKSSGGLEESAVTGIVVGALLGAGLLMAFYFFRKKYRITIERRNHQEESNIGKHRELREDSIRSHFSVA

Target ORF information:

RefSeq Version	XM_006232045.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus natriuretic peptide receptor 3 (Npr3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232045.3

ORF Insert Sequence:

ATGTGCAAAG GAAGGGAGAA TATATATATA TACATATATA TATATATATA TATATATATA 
TATATATTTA TAATAAGTCT ATATGTATAT TACAGACGCA CAGGTTTACA TTCTGTGAAC 
TTTTTTCTTT TTTCTTTCTT TGCTTTTTTT TTTTTTAAGC CTAGGGATAT TGCAGAGGAG 
TCTTCCTCTC TATCTTTTGG CGCGTTACTG AAGGGGGTAT TCTGTTTTCT TGGAGCGCCT 
AAGGGGGCGC AGGGACAGAG GAAAGAAAAG AGCAGGGAGG AAAGGGGCAG GGTGGGTGGG 
GGGCAGAGGG CGAGCCGGTG GCCAGGGCGA GCGCTTGCTG GCAACAGGAT GCGGTCCCTG 
CTGCTGTTCA CTTTCTCGGC GTGCGTGCTG CTGGCCCGGG CGCTGCTGGC TGGCGGTGCG 
AGTAGTGGCG GTGGGGACAC CGGGCCAGGC AACAGGCGCC GGGAGAGAGA GGCGCTGGCG 
GCTCAAAAGA TCGAGGTGCT TGTGCTATTG CCCCGGGACG ATTCGTACTT GTTCTCGCTG 
GCCCGGGTGA GGCCGGCCAT CGAGTACGCG CTGCGTAGCG TGGAGGGCAA TGGCACCGGC 
AGGAAGCTGC TGCCGCCCGG CACTCGCTTC CAGGTGGCCT ATGAAGACTC TGACTGTGGC 
AACCGCGCGC TCTTCAGTTT GGTGGACCGC GTGGCGGCGG CGCGCGGCGC CAAGCCGGAC 
CTCATCCTGG GTCCGGTGTG CGAGTACGCT GCGGCGCCGG TGGCCCGGCT GGCGTCTCAC 
TGGGACCTGC CGATGCTGTC CGCAGGAGCG CTGGCCGCGG GTTTCCAGCA CAAGGACACG 
GAGTACTCGC ACCTCACGCG CGTGGCGCCT GCCTACGCCA AGATGGGAGA GATGATGCTC 
GCTCTGTTCC GCCACCACCA CTGGAGCCGC GCTGCCCTGC TCTACAGCGA CGACAAACTC 
GAGAGAAACT GCTACTTCAC TCTCGAGGGG GTCCACGAGG TTTTCCAGGA GGAGGGCTTG 
CACACGTCTG CCTACAATTT CGACGAGACC AAAGACTTGG ACCTGGACGA CATAGTGCGC 
TACATCCAGG GCAGTGAGCG AGTGGTGATC ATGTGTGCCA GTGGTGACAC CATTCGGAGA 
ATCATGTTGG CGGTGCACAG ACACGGCATG ACCAGTGGAG ACTACGCTTT CTTCAACATT 
GAACTCTTCA ACAGTTCTTC TTACGGAGAT GGCTCGTGGA AGAGAGGGGA CAAACACGAC 
TTTGAAGCTA AGCAAGCTTA CTCATCCCTC CAAACAGTCA CCCTGCTGAG GACCGCGAAG 
CCTGAGTTTG AGAAGTTTTC CATGGAGGTG AAAAGTTCTG TTGAGAAACA AGGGCTCAAT 
GAGGAGGATT ACGTGAACAT GTTCGTTGAA GGCTTCCATG ATGCCATCCT CCTCTACGTT 
CTGGCTTTGC ATGAAGTGCT CAGAGCTGGC TACAGCAAGA AGGATGGGGG GAAAATCATC 
CAGCAGACTT GGAACAGGAC ATTTGAAGGT ATCGCCGGGC AGGTGTCCAT AGATGCTAAT 
GGGGATCGGT ATGGAGACTT CTCTGTGGTT GCCATGACTG ATACAGAAGC AGGTACCCAG 
GAGGTCATTG GTGATTACTT TGGAAAAGAA GGCCGGTTCA AAATGCGATC CAATGTCAAA 
TATCCTTGGG GCTCTTTGAA ATTGAGAATA GATGAAACCA GAATTGTGGA GCACACCAAC 
AGCTCTCCTT GCAAATCATC AGGTGGCCTA GAAGAATCAG CAGTGACAGG AATTGTTGTG 
GGGGCCCTAC TAGGTGCTGG TTTGCTAATG GCCTTCTACT TTTTCAGGAA GAAATACAGA 
ATAACCATTG AGAGGCGAAA TCACCAAGAA GAAAGCAACA TTGGGAAACA TCGAGAGCTG 
CGGGAAGATT CCATCAGATC ACATTTTTCG GTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-?.
Cell cycle (Georgetown, Tex.)15(18)2414-9(2016 Sep)
Lin D,Chai Y,Izadpanah R,Braun SE,Alt E

Transient silencing of Npr3 gene expression improved the circulatory levels of atrial natriuretic peptides and attenuated ?-adrenoceptor activation- induced cardiac hypertrophic growth in experimental rats.
European journal of pharmacology78244-58(2016 Jul)
Venkatesan B,Tumala A,Subramanian V,Vellaichamy E

Natriuretic peptide receptor 3 (NPR3) is regulated by microRNA-100.
Journal of molecular and cellular cardiology8213-21(2015 May)
Wong LL,Wee AS,Lim JY,Ng JY,Chong JP,Liew OW,Lilyanna S,Martinez EC,Ackers-Johnson MA,Vardy LA,Armugam A,Jeyaseelan K,Ng TP,Lam CS,Foo RS,Richards AM,Chen YT

Paradoxical expressions of natriuretic peptide receptor-C and neutral endopeptidase account for C-type natriuretic peptide decline during the progression of experimental obstructive nephropathy.
Journal of the renin-angiotensin-aldosterone system : JRAAS15(4)458-65(2014 Dec)
Hu P,Zhao XQ,Wang J,Kong HB,Hu B,Lu L,Qin YH