Pld1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pld1
Entrez Gene ID	25096
Full Name	phospholipase D1
Synonyms	Pld1a,Pld1b,Plda,Pldb
General protein information	Preferred Names phospholipase D1 Names phospholipase D1 PLD 1 Phoshpolipase D gene 1 probably with two splicing variants A and B choline phosphatase 1 phosphatidylcholine-hydrolyzing phospholipase D1 phospholipase D gene 1, probably with two splicing variants A and B phospholipase D1, phosphatidylcholine-specific rPLD1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q24
Summary	ubiquitous enzyme that hydrolyzes phosphatidylcholine to phosphatidic acid (PA) and choline [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006232206.3	XP_006232268.1	phospholipase D1 isoform X1
XM_008760891.2	XP_008759113.1	phospholipase D1 isoform X2
XM_017590637.1	XP_017446126.1	phospholipase D1 isoform X1
NM_030992.1	NP_112254.1	phospholipase D1
XM_006232205.3	XP_006232267.1	phospholipase D1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PLD1	NP_002653.1
Canis lupus familiaris (dog)	PLD1	XP_005640011.1
Mus musculus (house mouse)	Pld1	NP_032901.2
Rattus norvegicus (Norway rat)	Pld1	NP_112254.1
Danio rerio (zebrafish)	pld1b	XP_002666720.2
	PLD1	XP_001085464.2
Gallus gallus (chicken)	PLD1	XP_004943530.1
Xenopus tropicalis (tropical clawed frog)	pld1	XP_004914479.1
Pan troglodytes (chimpanzee)	PLD1	XP_003310147.1
Danio rerio (zebrafish)	pld1a	NP_001153567.1
Arabidopsis thaliana (thale cress)	PLDP2	NP_187214.2
Caenorhabditis elegans (roundworm)	pld-1	NP_494939.1
Bos taurus (cattle)	PLD1	NP_001095471.1
Arabidopsis thaliana (thale cress)	PLDP1	NP_188302.2

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of PLD1 activity causes ER stress via regulation of COPII vesicle formation.
Nakagawa H, Hazama K, Ishida K, Komori M, Nishimura K, Matsuo S
Biochemical and biophysical research communications490(3)895-900(2017 08)

PLD1 promotes dendritic spine development by inhibiting ADAM10-mediated N-cadherin cleavage.
Luo LD, Li G, Wang Y
Scientific reports7(1)6035(2017 Jul)

Simultaneous high expression of PLD1 and Sp1 predicts a poor prognosis for pancreatic ductal adenocarcinoma patients.
Hu J, Hu H, Hang JJ, Yang HY, Wang ZY, Wang L, Chen DH, Wang LW
Oncotarget7(48)78557-78565(2016 Nov)

Phospholipase D1 increases Bcl-2 expression during neuronal differentiation of rat neural stem cells.
Park SY, Ma W, Yoon SN, Kang MJ, Han JS
Molecular neurobiology51(3)1089-102(2015)

The role of phospholipase D1 in liver fibrosis induced by dimethylnitrosamine in vivo.
Zhu X, Liu R, Kuang D, Liu J, Shi X, Zhang T, Zeng Y, Sun X, Zhang Y, Yang W
Digestive diseases and sciences59(8)1779-88(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that PLD1 activity regulates COPII vesicle transport from the endoplasmic reticulum (ER) to the Golgi apparatus by regulating Sec13/31 recruitment from the cytosol to the ER membrane during COPII vesicle formation.
Title: Inhibition of PLD1 activity causes ER stress via regulation of COPII vesicle formation.

During neuronal differentiation of neural stem cells, Pld1 is activated.
Title: Phospholipase D1 increases Bcl-2 expression during neuronal differentiation of rat neural stem cells.

PLD plays an important role in the development and progression of rat liver fibrosis
Title: The role of phospholipase D1 in liver fibrosis induced by dimethylnitrosamine in vivo.

Results show that TRPM8 and the 5-HT1BR interact directly in a novel signalling complex that incorporates PLD1, which mediates the facilitatory influence by means of its product phosphatidic acid acting on PIP 5-kinase to increase production of PIP2
Title: The TRPM8 channel forms a complex with the 5-HT(1B) receptor and phospholipase D that amplifies its reversal of pain hypersensitivity.

Phospholipase D1 (PLD1)-mediated signalling, that may involve 5-HT2A receptor (5-HT2AR) activation, at both levels of the nervous system, is reported.
Title: Phospholipase D-mediated hypersensitivity at central synapses is associated with abnormal behaviours and pain sensitivity in rats exposed to prenatal stress.

The following Pld1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pld1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07292	NM_030992.1 Latest version!	Rattus norvegicus phospholipase D1 (Pld1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa24574	XM_006232205.3 Latest version!	Rattus norvegicus phospholipase D1 (Pld1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa24574	XM_006232206.3 Latest version!	Rattus norvegicus phospholipase D1 (Pld1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa24574	XM_017590637.1 Latest version!	Rattus norvegicus phospholipase D1 (Pld1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa43013	XM_008760891.2 Latest version!	Rattus norvegicus phospholipase D1 (Pld1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pld1 ( NM_030992.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112254.1 Rattus norvegicus phospholipase D1 (Pld1), mRNA.

Price & Availability↑

CloneID	ORa07292
Clone ID Related Accession (Same CDS sequence)	NM_030992.1
Accession Version	NM_030992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	phospholipase D1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U69550.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U69550.1, AB003171.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END##

ATGTCACTAA GAAGTGAGGC CCGGGTGAAC ACTTCTACAC TGCAGAAGAT CGCCGCAGAT 
ATGAGAAACC TGATAGAAAA TCTCGACACC CGGGAACTTC ATTTTGAGGG AGAGGAGGTG 
GAGTATGATG CATCTCCAGG AGACCCCACG GCTCAGGAAG CGTGCATCCC ATTCTCTTCT 
ATTTACAACA CTCAAGGATT TAAGGAGCCC AACATCCAGA TCTACCTCTC TGGCTGTCCT 
GTAAAAGCAC AAGTACTGGA AGTGGAGCGC TTCACGTCGA CCTCGAGGAT GCCAAGTGTC 
AACCTGTACA CCATCGAGTT AACACACGGG GAATTCACAT GGCAAGTTAA GAGGAAATTC 
AAGCACTTCC AAGAGTTTCA TAGAGAGCTC CTCAAGTACA AAGCCTTCAT CCGGATCCCC 
ATTCCCACCA AAAGACATAC GTTTAGAAGA CAAAATGTCA AAGAAGAGCC CAGAGAAATG 
CCCAGTCTGC CCCGTTCATC AGAAAATGCA ATCCAGGAGG AGCAGTTCTT TGGCAGGAGG 
AAGCAACTGG AAGATTACTT GACAAAAATA CTAAAAATGC CCATGTACAG AAACTATCAT 
GCCACGACAG AGTTCCTTGA TGTAAGCCAA CTGTCTTTTA TCCATGATTT GGGACCAAAG 
GGCCTGGAAG GAATGATCAT GAAAAGATCT GGGGGACACA GGATACCAGG TGTGAATTGC 
TGTGGCCATG GAAGAGCCTG CTACCGGTGG TCAAAGAGGT GGTTGATAGT GAAAGACTCT 
TTCCTGCTGT ATATGAAACC AGACAGCGGG GCGATCGCCT TCGTCCTTCT TGTAGACAAA 
GAATTCAGAA TCAAAGTGGG AAAGAAGGAG ACAGAAACGA AATATGGACT TCGAATTGAT 
AATCTTTCAA GGACACTGAT TTTAAAATGT AACAGCTACA GACATGCCCG GTGGTGGGGA 
GGCGCCATCG AGGAGTTCAT CCAGAAGCAC GGCACTGACT TCCTCAAAGA CCATCGCTTC 
GGCTCCTACG CTGCGGTCCA CGAGAACATT TTAGCTAAAT GGTACGTGAA CGCCAAAGGT 
TACTTTGAAG ATATCGCAAA CGCAATGGAA GGGGCAACGG AAGAGATTTT TATCACAGAT 
TGGTGGTTAA GTCCAGAAAT CTTCCTGAAG CGCCCAGTGG TGGAAGGCAA CCGCTGGAGG 
CTGGACTGCA TCCTCAAACG GAAAGCACAA CAAGGTGTGC GGATTTTCAT AATGCTTTAC 
AAAGAGGTGG AGCTAGCCCT CGGGATCAAC AGCGAGTACA CCAAGCGGAC TTTGATGCGG 
CTGCACCCCA ACATAAAGGT GATGAGACAC CCAGACCATG TGTCCTCCAG TGTGTATCTG 
TGGGCTCATC ACGAGAAGCT CGTCATTATC GACCAATCGG TGGCGTTTGT GGGTGGGATT 
GACCTGGCCT ATGGACGGTG GGATGACAAT GAGCACAGAC TCACAGATGT GGGCAGTGTC 
AAGCGGGTCA CCTCAGGACA GTCTCTGGGC TCTCTCACAG CAGCATCAGT AGAGTCTATG 
GAATCCTTAA GCCTCAAGGA CAAACATCAA TCTCATAAAA ACGAGCCCGT CTTAAAGAGT 
GTCAACGATA CCGACATGAA ACTGAAAGGC ATAGGGAAGT CCAGGAAATT CTCCAAATTT 
AGCCTCTATC GCCAACTTCA CCGCCGTAAT CTGCACAACT CGGACAGCAT TAGCAGCGTC 
GACAGCGCCT CCAACACCGG CTCCATCCGA AGTGTGCAGA CAGGAGTGGG AGAGCTCCAT 
GGGGAGACTA GGTTTTGGCA TGGGAAGGAT TACTGCAACT TTGTCTTCAA GGACTGGGTT 
CAACTGGACA AACCTTTTGC TGATTTCATC GACAGGTACT CCACCCCCCG GATGCCTTGG 
CATGACATTG GTTCTGTGGT TCACGGAAAG GCAGCTCGTG ACGTGGCTCG ACACTTCATC 
CAGCGCTGGA ACTTCACAAA GATTATGAAG CCAAAATATC GTTCCCTTTC TTATCCTTTC 
CTGCTCCCAA AGTCTCAAGC CACAGCGCAT GAGCTGAGGT ATCAGGTGCC AGGGGCCGTC 
CACGCCAAAG CGCAGTTGCT CCGATCTGCT GCTGACTGGT CTGCTGGTAT AAAGCANCAC 
GAGGAATCTA TCCATGCTGC CTACACCCAT GTCATAGAGA ACAGCAAGCA CTACATCTAT 
ATTGAAAACC AATTTTTCAT AAGCTGCGCT GATGATAAAG TCGTGTTCAA CAAGGTGGGC 
AATGCTATCG CCCAGAGAAT CCTGAAAGCC CACAGGGAAG GCCAGAGATA TCGAGTGTAC 
ATCGTGATAC CACTGCTGCC GGGATTCGAA GGAGACATTT CCACAGGCGG GGGAAATGCC 
CTACAGGCAA TAATGCACTT CAACTACAGA ACCATGTGCA GAGGGGAGAG CTCCATCCTT 
GAACAGTTAA AGCCAGAGCT TGGTAACAAG TGGATAAATT ACATATCATT TTGCGGTCTG 
AGAACACATG CAGAGCTGGA AGGAAACCTT GTCACTGAGC TCATCTATGT CCACAGCAAG 
TTGTTAATTG CTGATGATAA CACGGTTATC ATAGGTTCTG CCAACATCAA TGACCGGAGC 
ATGCTGGGAA AGCGAGACAG CGAAATGGCT GTCATAGTGC AGGACAGACA GACAGTCCCG 
TCAGTGATGG ATGGAAAAGA ATATCAAGCT GGCCGCTTCG CTCAAGGCCT TCGGCTGGAG 
TGCTTCAGGC TTGTCCTGGG TTATCTTTCT GACCCAAGTG AGGACATTCA GGATCCTGTA 
AGTGACAAAT TCTTCAAGGA GATTTGGGTT TCAACGGCAG CTCGAAATGC TACCATATAT 
GATAAGGTCT TCCGGTGCCT TCCCAATGAC GAAGTGCACA ATTTAATCCA GTTGCGGGAC 
TTTATAAACA AGCCTATATT GGCAAAAGAG GACCGCCTTC GTGCCGAGGA AGAACTGAGG 
AAGATCCGTG GCTTCTTGGT GCAGTTCCCC TTCTACTTCT TATCAGAAGA AAACCTGCTG 
CCTTCCGTTG GAACCAAGGA AGCCATTGTG CCCATGGAGG TTTGGACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112254.1
CDS	181..3291
Translation	MSLRSEARVNTSTLQKIAADMRNLIENLDTRELHFEGEEVEYDASPGDPTAQEACIPFSSIYNTQGFKEPNIQIYLSGCPVKAQVLEVERFTSTSRMPSVNLYTIELTHGEFTWQVKRKFKHFQEFHRELLKYKAFIRIPIPTKRHTFRRQNVKEEPREMPSLPRSSENAIQEEQFFGRRKQLEDYLTKILKMPMYRNYHATTEFLDVSQLSFIHDLGPKGLEGMIMKRSGGHRIPGVNCCGHGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDKEFRIKVGKKETETKYGLRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTDFLKDHRFGSYAAVHENILAKWYVNAKGYFEDIANAMEGATEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRVTSGQSLGSLTAASVESMESLSLKDKHQSHKNEPVLKSVNDTDMKLKGIGKSRKFSKFSLYRQLHRRNLHNSDSISSVDSASNTGSIRSVQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIGSVVHGKAARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQATAHELRYQVPGAVHAKAQLLRSAADWSAGIKnullHEESIHAAYTHVIENSKHYIYIENQFFISCADDKVVFNKVGNAIAQRILKAHREGQRYRVYIVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGESSILEQLKPELGNKWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDRQTVPSVMDGKEYQAGRFAQGLRLECFRLVLGYLSDPSEDIQDPVSDKFFKEIWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPILAKEDRLRAEEELRKIRGFLVQFPFYFLSEENLLPSVGTKEAIVPMEVWT

Target ORF information:

RefSeq Version	NM_030992.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase D1 (Pld1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030992.1

ORF Insert Sequence:

ATGTCACTAA GAAGTGAGGC CCGGGTGAAC ACTTCTACAC TGCAGAAGAT CGCCGCAGAT 
ATGAGAAACC TGATAGAAAA TCTCGACACC CGGGAACTTC ATTTTGAGGG AGAGGAGGTG 
GAGTATGATG CATCTCCAGG AGACCCCACG GCTCAGGAAG CGTGCATCCC ATTCTCTTCT 
ATTTACAACA CTCAAGGATT TAAGGAGCCC AACATCCAGA TCTACCTCTC TGGCTGTCCT 
GTAAAAGCAC AAGTACTGGA AGTGGAGCGC TTCACGTCGA CCTCGAGGAT GCCAAGTGTC 
AACCTGTACA CCATCGAGTT AACACACGGG GAATTCACAT GGCAAGTTAA GAGGAAATTC 
AAGCACTTCC AAGAGTTTCA TAGAGAGCTC CTCAAGTACA AAGCCTTCAT CCGGATCCCC 
ATTCCCACCA AAAGACATAC GTTTAGAAGA CAAAATGTCA AAGAAGAGCC CAGAGAAATG 
CCCAGTCTGC CCCGTTCATC AGAAAATGCA ATCCAGGAGG AGCAGTTCTT TGGCAGGAGG 
AAGCAACTGG AAGATTACTT GACAAAAATA CTAAAAATGC CCATGTACAG AAACTATCAT 
GCCACGACAG AGTTCCTTGA TGTAAGCCAA CTGTCTTTTA TCCATGATTT GGGACCAAAG 
GGCCTGGAAG GAATGATCAT GAAAAGATCT GGGGGACACA GGATACCAGG TGTGAATTGC 
TGTGGCCATG GAAGAGCCTG CTACCGGTGG TCAAAGAGGT GGTTGATAGT GAAAGACTCT 
TTCCTGCTGT ATATGAAACC AGACAGCGGG GCGATCGCCT TCGTCCTTCT TGTAGACAAA 
GAATTCAGAA TCAAAGTGGG AAAGAAGGAG ACAGAAACGA AATATGGACT TCGAATTGAT 
AATCTTTCAA GGACACTGAT TTTAAAATGT AACAGCTACA GACATGCCCG GTGGTGGGGA 
GGCGCCATCG AGGAGTTCAT CCAGAAGCAC GGCACTGACT TCCTCAAAGA CCATCGCTTC 
GGCTCCTACG CTGCGGTCCA CGAGAACATT TTAGCTAAAT GGTACGTGAA CGCCAAAGGT 
TACTTTGAAG ATATCGCAAA CGCAATGGAA GGGGCAACGG AAGAGATTTT TATCACAGAT 
TGGTGGTTAA GTCCAGAAAT CTTCCTGAAG CGCCCAGTGG TGGAAGGCAA CCGCTGGAGG 
CTGGACTGCA TCCTCAAACG GAAAGCACAA CAAGGTGTGC GGATTTTCAT AATGCTTTAC 
AAAGAGGTGG AGCTAGCCCT CGGGATCAAC AGCGAGTACA CCAAGCGGAC TTTGATGCGG 
CTGCACCCCA ACATAAAGGT GATGAGACAC CCAGACCATG TGTCCTCCAG TGTGTATCTG 
TGGGCTCATC ACGAGAAGCT CGTCATTATC GACCAATCGG TGGCGTTTGT GGGTGGGATT 
GACCTGGCCT ATGGACGGTG GGATGACAAT GAGCACAGAC TCACAGATGT GGGCAGTGTC 
AAGCGGGTCA CCTCAGGACA GTCTCTGGGC TCTCTCACAG CAGCATCAGT AGAGTCTATG 
GAATCCTTAA GCCTCAAGGA CAAACATCAA TCTCATAAAA ACGAGCCCGT CTTAAAGAGT 
GTCAACGATA CCGACATGAA ACTGAAAGGC ATAGGGAAGT CCAGGAAATT CTCCAAATTT 
AGCCTCTATC GCCAACTTCA CCGCCGTAAT CTGCACAACT CGGACAGCAT TAGCAGCGTC 
GACAGCGCCT CCAACACCGG CTCCATCCGA AGTGTGCAGA CAGGAGTGGG AGAGCTCCAT 
GGGGAGACTA GGTTTTGGCA TGGGAAGGAT TACTGCAACT TTGTCTTCAA GGACTGGGTT 
CAACTGGACA AACCTTTTGC TGATTTCATC GACAGGTACT CCACCCCCCG GATGCCTTGG 
CATGACATTG GTTCTGTGGT TCACGGAAAG GCAGCTCGTG ACGTGGCTCG ACACTTCATC 
CAGCGCTGGA ACTTCACAAA GATTATGAAG CCAAAATATC GTTCCCTTTC TTATCCTTTC 
CTGCTCCCAA AGTCTCAAGC CACAGCGCAT GAGCTGAGGT ATCAGGTGCC AGGGGCCGTC 
CACGCCAAAG CGCAGTTGCT CCGATCTGCT GCTGACTGGT CTGCTGGTAT AAAGCANCAC 
GAGGAATCTA TCCATGCTGC CTACACCCAT GTCATAGAGA ACAGCAAGCA CTACATCTAT 
ATTGAAAACC AATTTTTCAT AAGCTGCGCT GATGATAAAG TCGTGTTCAA CAAGGTGGGC 
AATGCTATCG CCCAGAGAAT CCTGAAAGCC CACAGGGAAG GCCAGAGATA TCGAGTGTAC 
ATCGTGATAC CACTGCTGCC GGGATTCGAA GGAGACATTT CCACAGGCGG GGGAAATGCC 
CTACAGGCAA TAATGCACTT CAACTACAGA ACCATGTGCA GAGGGGAGAG CTCCATCCTT 
GAACAGTTAA AGCCAGAGCT TGGTAACAAG TGGATAAATT ACATATCATT TTGCGGTCTG 
AGAACACATG CAGAGCTGGA AGGAAACCTT GTCACTGAGC TCATCTATGT CCACAGCAAG 
TTGTTAATTG CTGATGATAA CACGGTTATC ATAGGTTCTG CCAACATCAA TGACCGGAGC 
ATGCTGGGAA AGCGAGACAG CGAAATGGCT GTCATAGTGC AGGACAGACA GACAGTCCCG 
TCAGTGATGG ATGGAAAAGA ATATCAAGCT GGCCGCTTCG CTCAAGGCCT TCGGCTGGAG 
TGCTTCAGGC TTGTCCTGGG TTATCTTTCT GACCCAAGTG AGGACATTCA GGATCCTGTA 
AGTGACAAAT TCTTCAAGGA GATTTGGGTT TCAACGGCAG CTCGAAATGC TACCATATAT 
GATAAGGTCT TCCGGTGCCT TCCCAATGAC GAAGTGCACA ATTTAATCCA GTTGCGGGAC 
TTTATAAACA AGCCTATATT GGCAAAAGAG GACCGCCTTC GTGCCGAGGA AGAACTGAGG 
AAGATCCGTG GCTTCTTGGT GCAGTTCCCC TTCTACTTCT TATCAGAAGA AAACCTGCTG 
CCTTCCGTTG GAACCAAGGA AGCCATTGTG CCCATGGAGG TTTGGACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pld1 ( XM_006232206.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232268.1 Rattus norvegicus phospholipase D1 (Pld1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24574
Clone ID Related Accession (Same CDS sequence)	XM_006232206.3 , XM_017590637.1 , XM_006232205.3
Accession Version	XM_006232206.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholipase D1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232206.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTAA AAAGTGAGGC CCGGGTGAAC ACTTCTACAC TGCAGAAGAT CGCCGCAGAT 
ATGAGCAACC TGATAGAAAA TCTCGACACC CGGGAACTTC ACTTTGAGGG AGAGGAGGTG 
GAGTATGATG CATCTCCAGG AGACCCCACG GCTCAGGAAG CGTGCATCCC ATTCTCTTCT 
ATTTACAACA CTCAAGGATT TAAGGAGCCC AACATCCAGA TCTACCTCTC TGGCTGTCCT 
GTAAAAGCAC AAGTACTGGA AGTGGAGCGC TTCACGTCGA CCTCGAGGAT GCCAAGTGTC 
AACCTGTACA CCATCGAGTT AACACACGGG GAATTCACAT GGCAAGTTAA GAGGAAATTC 
AAGCACTTCC AAGAGTTTCA TAGAGAGCTC CTCAAGTACA AAGCCTTCAT CCGGATCCCC 
ATTCCCACCA AAAGACATAC GTTTAGAAGA CAAAATGTCA AAGAAGAGCC CAGAGAAATG 
CCCAGTCTGC CCCGTTCATC AGAAAATGCA ATCCAGGAGG AGCAGTTCTT TGGCAGGAGG 
AAGCAACTGG AAGATTACTT GACAAAAATA CTAAAAATGC CCATGTACAG AAACTATCAT 
GCCACGACAG AGTTCCTTGA TGTAAGCCAA CTGTCTTTTA TCCATGATTT GGGACCAAAG 
GGCCTGGAAG GAATGATCAT GAAAAGATCT GGGGGACACA GGATACCAGG TGTGAATTGC 
TGTGGCCATG GAAGAGCCTG CTACCGGTGG TCAAAGAGGT GGTTGATAGT GAAAGACTCT 
TTCCTGCTGT ATATGAAACC AGACAGCGGG GCGATCGCCT TCGTCCTTCT TGTAGACAAA 
GAATTCAGAA TCAAAGTGGG AAAGAAGGAG ACAGAAACGA AATATGGACT TCGAATTGAT 
AATCTTTCAA GGACACTGAT TTTAAAATGT AACAGCTACA GACATGCCCG GTGGTGGGGA 
GGCGCCATCG AGGAGTTCAT CCAGAAGCAC GGCACTGACT TCCTCAAAGA CCATCGCTTC 
GGCTCCTACG CTGCGGTCCA CGAGAACATT TTAGCTAAAT GGTACGTGAA CGCCAAAGGT 
TACTTTGAAG ATATCGCAAA CGCAATGGAA GGGGCAACGG AAGAGATTTT TATCACAGAT 
TGGTGGTTAA GTCCAGAAAT CTTCCTGAAG CGCCCAGTGG TGGAAGGCAA CCGCTGGAGG 
CTGGACTGCA TCCTCAAACG GAAAGCACAA CAAGGTGTGC GGATTTTCAT AATGCTTTAC 
AAAGAGGTGG AGCTAGCCCT CGGGATCAAC AGCGAGTACA CCAAGCGGAC TTTGATGCGG 
CTGCACCCCA ACATAAAGGT GATGAGACAC CCAGACCATG TGTCCTCCAG TGTGTATCTG 
TGGGCTCATC ACGAGAAGCT CGTCATTATC GACCAATCGG TGGCGTTTGT GGGTGGGATT 
GACCTGGCCT ATGGACGGTG GGATGACAAT GAGCACAGAC TCACAGATGT GGGCAGTGTC 
AAGCGGGTCA CCTCAGGACA GTCTCTGGGC TCTCTCACAG CAGCATCAGT AGAGTCTATG 
GAATCCTTAA GCCTCAAGGA CAAACATCAA TCTCATAAAA ACGAGCCCGT CTTAAAGAGT 
GTCAACGATA CCGACATGAA ACTGAAAGGC ATAGGGAAGT CCAGGAAATT CTCCAAATTT 
AGCCTCTATC GCCAACTTCA CCGCCGTAAT CTGCACAACT CGGACAGCAT TAGCAGCGTC 
GACAGCGCTT CCAGTTATTT TAACCACTAT AGAAGTCATC AGAATTTAAT CCATGGGTTA 
AAGCCCCACT TGAAACTCTT TCGCCCTTCC AGTGAGTCTG AGCAGGGCCT CACTAGGCAC 
AGTGCTGACA CCGGCTCCAT CCGAAGTGTG CAGACAGGAG TGGGAGAGCT CCATGGGGAG 
ACTAGGTTTT GGCATGGGAA GGATTACTGC AACTTTGTCT TCAAGGACTG GGTTCAACTG 
GACAAACCTT TTGCTGATTT CATCGACAGG TACTCCACCC CCCGGATGCC TTGGCATGAC 
ATTGGTTCTG TGGTTCACGG AAAGGCAGCT CGTGACGTGG CTCGACACTT CATCCAGCGC 
TGGAACTTCA CAAAGATTAT GAAGCCAAAA TATCGTTCCC TTTCTTATCC TTTCCTGCTC 
CCAAAGTCTC AAGCCACAGC GCATGAGCTG AGGTATCAGG TGCCAGGGGC CGTCCACGCC 
AAAGCACAGT TGCTCCGATC TGCTGCTGAC TGGTCTGCTG GTATAAAGCA CCACGAGGAA 
TCTATCCATG CTGCCTACAC CCATGTCATA GAGAACAGCA AGCACTACAT CTATATTGAA 
AACCAATTTT TCATAAGCTG CGCTGATGAT AAAGTCGTGT TCAACAAGGT GGGCAATGCT 
ATCGCCCAGA GAATCCTGAA AGCCCACAGG GAAGGCCAGA GATATCGAGT GTACATCGTG 
ATACCACTGC TGCCGGGATT CGAAGGAGAC ATTTCCACAG GCGGGGGAAA TGCCCTACAG 
GCAATAATGC ACTTCAACTA CAGAACCATG TGCAGAGGGG AGAGCTCCAT CCTTGAACAG 
TTAAAGCCAG AGCTTGGTAA CAAGTGGATA AATTACATAT CATTTTGCGG TCTGAGAACA 
CATGCAGAGC TGGAAGGAAA CCTTGTCACT GAGCTCATCT ATGTCCACAG CAAGTTGTTA 
ATTGCTGATG ATAACACGGT TATCATAGGT TCTGCCAACA TCAATGACCG GAGCATGCTG 
GGAAAGCGAG ACAGCGAAAT GGCTGTCATA GTGCAGGACA CAGAGACAGT CCCGTCAGTG 
ATGGATGGAA AAGAATATCA AGCTGGCCGC TTCGCTCAAG GCCTTCGGCT GGAGTGCTTC 
AGGCTTGTCC TGGGTTATCT TTCTGACCCA AGTGAGGACA TTCAGGATCC TGTAAGTGAC 
AAATTCTTCA AGGAGATTTG GGTTTCAACG GCAGCTCGAA ATGCTACCAT ATATGATAAG 
GTCTTCCGGT GCCTTCCCAA TGACGAAGTG CACAATTTAA TCCAGTTGCG GGACTTTATA 
AACAAGCCTA TATTGGCAAA AGAGGACCGC CTTCGTGCCG AGGAAGAACT GAGGAAGATC 
CGTGGCTTCT TGGTGCAGTT CCCCTTCTAC TTCTTATCAG AAGAAAACCT GCTGCCTTCC 
GTTGGAACCA AGGAAGCCAT TGTGCCCATG GAGGTTTGGA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232268.1
CDS	606..3830
Translation	MSLKSEARVNTSTLQKIAADMSNLIENLDTRELHFEGEEVEYDASPGDPTAQEACIPFSSIYNTQGFKEPNIQIYLSGCPVKAQVLEVERFTSTSRMPSVNLYTIELTHGEFTWQVKRKFKHFQEFHRELLKYKAFIRIPIPTKRHTFRRQNVKEEPREMPSLPRSSENAIQEEQFFGRRKQLEDYLTKILKMPMYRNYHATTEFLDVSQLSFIHDLGPKGLEGMIMKRSGGHRIPGVNCCGHGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDKEFRIKVGKKETETKYGLRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTDFLKDHRFGSYAAVHENILAKWYVNAKGYFEDIANAMEGATEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRVTSGQSLGSLTAASVESMESLSLKDKHQSHKNEPVLKSVNDTDMKLKGIGKSRKFSKFSLYRQLHRRNLHNSDSISSVDSASSYFNHYRSHQNLIHGLKPHLKLFRPSSESEQGLTRHSADTGSIRSVQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIGSVVHGKAARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQATAHELRYQVPGAVHAKAQLLRSAADWSAGIKHHEESIHAAYTHVIENSKHYIYIENQFFISCADDKVVFNKVGNAIAQRILKAHREGQRYRVYIVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGESSILEQLKPELGNKWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFAQGLRLECFRLVLGYLSDPSEDIQDPVSDKFFKEIWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPILAKEDRLRAEEELRKIRGFLVQFPFYFLSEENLLPSVGTKEAIVPMEVWT

Target ORF information:

RefSeq Version	XM_006232206.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase D1 (Pld1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232206.3

ORF Insert Sequence:

ATGTCACTAA AAAGTGAGGC CCGGGTGAAC ACTTCTACAC TGCAGAAGAT CGCCGCAGAT 
ATGAGCAACC TGATAGAAAA TCTCGACACC CGGGAACTTC ACTTTGAGGG AGAGGAGGTG 
GAGTATGATG CATCTCCAGG AGACCCCACG GCTCAGGAAG CGTGCATCCC ATTCTCTTCT 
ATTTACAACA CTCAAGGATT TAAGGAGCCC AACATCCAGA TCTACCTCTC TGGCTGTCCT 
GTAAAAGCAC AAGTACTGGA AGTGGAGCGC TTCACGTCGA CCTCGAGGAT GCCAAGTGTC 
AACCTGTACA CCATCGAGTT AACACACGGG GAATTCACAT GGCAAGTTAA GAGGAAATTC 
AAGCACTTCC AAGAGTTTCA TAGAGAGCTC CTCAAGTACA AAGCCTTCAT CCGGATCCCC 
ATTCCCACCA AAAGACATAC GTTTAGAAGA CAAAATGTCA AAGAAGAGCC CAGAGAAATG 
CCCAGTCTGC CCCGTTCATC AGAAAATGCA ATCCAGGAGG AGCAGTTCTT TGGCAGGAGG 
AAGCAACTGG AAGATTACTT GACAAAAATA CTAAAAATGC CCATGTACAG AAACTATCAT 
GCCACGACAG AGTTCCTTGA TGTAAGCCAA CTGTCTTTTA TCCATGATTT GGGACCAAAG 
GGCCTGGAAG GAATGATCAT GAAAAGATCT GGGGGACACA GGATACCAGG TGTGAATTGC 
TGTGGCCATG GAAGAGCCTG CTACCGGTGG TCAAAGAGGT GGTTGATAGT GAAAGACTCT 
TTCCTGCTGT ATATGAAACC AGACAGCGGG GCGATCGCCT TCGTCCTTCT TGTAGACAAA 
GAATTCAGAA TCAAAGTGGG AAAGAAGGAG ACAGAAACGA AATATGGACT TCGAATTGAT 
AATCTTTCAA GGACACTGAT TTTAAAATGT AACAGCTACA GACATGCCCG GTGGTGGGGA 
GGCGCCATCG AGGAGTTCAT CCAGAAGCAC GGCACTGACT TCCTCAAAGA CCATCGCTTC 
GGCTCCTACG CTGCGGTCCA CGAGAACATT TTAGCTAAAT GGTACGTGAA CGCCAAAGGT 
TACTTTGAAG ATATCGCAAA CGCAATGGAA GGGGCAACGG AAGAGATTTT TATCACAGAT 
TGGTGGTTAA GTCCAGAAAT CTTCCTGAAG CGCCCAGTGG TGGAAGGCAA CCGCTGGAGG 
CTGGACTGCA TCCTCAAACG GAAAGCACAA CAAGGTGTGC GGATTTTCAT AATGCTTTAC 
AAAGAGGTGG AGCTAGCCCT CGGGATCAAC AGCGAGTACA CCAAGCGGAC TTTGATGCGG 
CTGCACCCCA ACATAAAGGT GATGAGACAC CCAGACCATG TGTCCTCCAG TGTGTATCTG 
TGGGCTCATC ACGAGAAGCT CGTCATTATC GACCAATCGG TGGCGTTTGT GGGTGGGATT 
GACCTGGCCT ATGGACGGTG GGATGACAAT GAGCACAGAC TCACAGATGT GGGCAGTGTC 
AAGCGGGTCA CCTCAGGACA GTCTCTGGGC TCTCTCACAG CAGCATCAGT AGAGTCTATG 
GAATCCTTAA GCCTCAAGGA CAAACATCAA TCTCATAAAA ACGAGCCCGT CTTAAAGAGT 
GTCAACGATA CCGACATGAA ACTGAAAGGC ATAGGGAAGT CCAGGAAATT CTCCAAATTT 
AGCCTCTATC GCCAACTTCA CCGCCGTAAT CTGCACAACT CGGACAGCAT TAGCAGCGTC 
GACAGCGCTT CCAGTTATTT TAACCACTAT AGAAGTCATC AGAATTTAAT CCATGGGTTA 
AAGCCCCACT TGAAACTCTT TCGCCCTTCC AGTGAGTCTG AGCAGGGCCT CACTAGGCAC 
AGTGCTGACA CCGGCTCCAT CCGAAGTGTG CAGACAGGAG TGGGAGAGCT CCATGGGGAG 
ACTAGGTTTT GGCATGGGAA GGATTACTGC AACTTTGTCT TCAAGGACTG GGTTCAACTG 
GACAAACCTT TTGCTGATTT CATCGACAGG TACTCCACCC CCCGGATGCC TTGGCATGAC 
ATTGGTTCTG TGGTTCACGG AAAGGCAGCT CGTGACGTGG CTCGACACTT CATCCAGCGC 
TGGAACTTCA CAAAGATTAT GAAGCCAAAA TATCGTTCCC TTTCTTATCC TTTCCTGCTC 
CCAAAGTCTC AAGCCACAGC GCATGAGCTG AGGTATCAGG TGCCAGGGGC CGTCCACGCC 
AAAGCACAGT TGCTCCGATC TGCTGCTGAC TGGTCTGCTG GTATAAAGCA CCACGAGGAA 
TCTATCCATG CTGCCTACAC CCATGTCATA GAGAACAGCA AGCACTACAT CTATATTGAA 
AACCAATTTT TCATAAGCTG CGCTGATGAT AAAGTCGTGT TCAACAAGGT GGGCAATGCT 
ATCGCCCAGA GAATCCTGAA AGCCCACAGG GAAGGCCAGA GATATCGAGT GTACATCGTG 
ATACCACTGC TGCCGGGATT CGAAGGAGAC ATTTCCACAG GCGGGGGAAA TGCCCTACAG 
GCAATAATGC ACTTCAACTA CAGAACCATG TGCAGAGGGG AGAGCTCCAT CCTTGAACAG 
TTAAAGCCAG AGCTTGGTAA CAAGTGGATA AATTACATAT CATTTTGCGG TCTGAGAACA 
CATGCAGAGC TGGAAGGAAA CCTTGTCACT GAGCTCATCT ATGTCCACAG CAAGTTGTTA 
ATTGCTGATG ATAACACGGT TATCATAGGT TCTGCCAACA TCAATGACCG GAGCATGCTG 
GGAAAGCGAG ACAGCGAAAT GGCTGTCATA GTGCAGGACA CAGAGACAGT CCCGTCAGTG 
ATGGATGGAA AAGAATATCA AGCTGGCCGC TTCGCTCAAG GCCTTCGGCT GGAGTGCTTC 
AGGCTTGTCC TGGGTTATCT TTCTGACCCA AGTGAGGACA TTCAGGATCC TGTAAGTGAC 
AAATTCTTCA AGGAGATTTG GGTTTCAACG GCAGCTCGAA ATGCTACCAT ATATGATAAG 
GTCTTCCGGT GCCTTCCCAA TGACGAAGTG CACAATTTAA TCCAGTTGCG GGACTTTATA 
AACAAGCCTA TATTGGCAAA AGAGGACCGC CTTCGTGCCG AGGAAGAACT GAGGAAGATC 
CGTGGCTTCT TGGTGCAGTT CCCCTTCTAC TTCTTATCAG AAGAAAACCT GCTGCCTTCC 
GTTGGAACCA AGGAAGCCAT TGTGCCCATG GAGGTTTGGA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pld1 ( XM_017590637.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446126.1 Rattus norvegicus phospholipase D1 (Pld1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa24574
Clone ID Related Accession (Same CDS sequence)	XM_006232206.3 , XM_017590637.1 , XM_006232205.3
Accession Version	XM_017590637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholipase D1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTAA AAAGTGAGGC CCGGGTGAAC ACTTCTACAC TGCAGAAGAT CGCCGCAGAT 
ATGAGCAACC TGATAGAAAA TCTCGACACC CGGGAACTTC ACTTTGAGGG AGAGGAGGTG 
GAGTATGATG CATCTCCAGG AGACCCCACG GCTCAGGAAG CGTGCATCCC ATTCTCTTCT 
ATTTACAACA CTCAAGGATT TAAGGAGCCC AACATCCAGA TCTACCTCTC TGGCTGTCCT 
GTAAAAGCAC AAGTACTGGA AGTGGAGCGC TTCACGTCGA CCTCGAGGAT GCCAAGTGTC 
AACCTGTACA CCATCGAGTT AACACACGGG GAATTCACAT GGCAAGTTAA GAGGAAATTC 
AAGCACTTCC AAGAGTTTCA TAGAGAGCTC CTCAAGTACA AAGCCTTCAT CCGGATCCCC 
ATTCCCACCA AAAGACATAC GTTTAGAAGA CAAAATGTCA AAGAAGAGCC CAGAGAAATG 
CCCAGTCTGC CCCGTTCATC AGAAAATGCA ATCCAGGAGG AGCAGTTCTT TGGCAGGAGG 
AAGCAACTGG AAGATTACTT GACAAAAATA CTAAAAATGC CCATGTACAG AAACTATCAT 
GCCACGACAG AGTTCCTTGA TGTAAGCCAA CTGTCTTTTA TCCATGATTT GGGACCAAAG 
GGCCTGGAAG GAATGATCAT GAAAAGATCT GGGGGACACA GGATACCAGG TGTGAATTGC 
TGTGGCCATG GAAGAGCCTG CTACCGGTGG TCAAAGAGGT GGTTGATAGT GAAAGACTCT 
TTCCTGCTGT ATATGAAACC AGACAGCGGG GCGATCGCCT TCGTCCTTCT TGTAGACAAA 
GAATTCAGAA TCAAAGTGGG AAAGAAGGAG ACAGAAACGA AATATGGACT TCGAATTGAT 
AATCTTTCAA GGACACTGAT TTTAAAATGT AACAGCTACA GACATGCCCG GTGGTGGGGA 
GGCGCCATCG AGGAGTTCAT CCAGAAGCAC GGCACTGACT TCCTCAAAGA CCATCGCTTC 
GGCTCCTACG CTGCGGTCCA CGAGAACATT TTAGCTAAAT GGTACGTGAA CGCCAAAGGT 
TACTTTGAAG ATATCGCAAA CGCAATGGAA GGGGCAACGG AAGAGATTTT TATCACAGAT 
TGGTGGTTAA GTCCAGAAAT CTTCCTGAAG CGCCCAGTGG TGGAAGGCAA CCGCTGGAGG 
CTGGACTGCA TCCTCAAACG GAAAGCACAA CAAGGTGTGC GGATTTTCAT AATGCTTTAC 
AAAGAGGTGG AGCTAGCCCT CGGGATCAAC AGCGAGTACA CCAAGCGGAC TTTGATGCGG 
CTGCACCCCA ACATAAAGGT GATGAGACAC CCAGACCATG TGTCCTCCAG TGTGTATCTG 
TGGGCTCATC ACGAGAAGCT CGTCATTATC GACCAATCGG TGGCGTTTGT GGGTGGGATT 
GACCTGGCCT ATGGACGGTG GGATGACAAT GAGCACAGAC TCACAGATGT GGGCAGTGTC 
AAGCGGGTCA CCTCAGGACA GTCTCTGGGC TCTCTCACAG CAGCATCAGT AGAGTCTATG 
GAATCCTTAA GCCTCAAGGA CAAACATCAA TCTCATAAAA ACGAGCCCGT CTTAAAGAGT 
GTCAACGATA CCGACATGAA ACTGAAAGGC ATAGGGAAGT CCAGGAAATT CTCCAAATTT 
AGCCTCTATC GCCAACTTCA CCGCCGTAAT CTGCACAACT CGGACAGCAT TAGCAGCGTC 
GACAGCGCTT CCAGTTATTT TAACCACTAT AGAAGTCATC AGAATTTAAT CCATGGGTTA 
AAGCCCCACT TGAAACTCTT TCGCCCTTCC AGTGAGTCTG AGCAGGGCCT CACTAGGCAC 
AGTGCTGACA CCGGCTCCAT CCGAAGTGTG CAGACAGGAG TGGGAGAGCT CCATGGGGAG 
ACTAGGTTTT GGCATGGGAA GGATTACTGC AACTTTGTCT TCAAGGACTG GGTTCAACTG 
GACAAACCTT TTGCTGATTT CATCGACAGG TACTCCACCC CCCGGATGCC TTGGCATGAC 
ATTGGTTCTG TGGTTCACGG AAAGGCAGCT CGTGACGTGG CTCGACACTT CATCCAGCGC 
TGGAACTTCA CAAAGATTAT GAAGCCAAAA TATCGTTCCC TTTCTTATCC TTTCCTGCTC 
CCAAAGTCTC AAGCCACAGC GCATGAGCTG AGGTATCAGG TGCCAGGGGC CGTCCACGCC 
AAAGCACAGT TGCTCCGATC TGCTGCTGAC TGGTCTGCTG GTATAAAGCA CCACGAGGAA 
TCTATCCATG CTGCCTACAC CCATGTCATA GAGAACAGCA AGCACTACAT CTATATTGAA 
AACCAATTTT TCATAAGCTG CGCTGATGAT AAAGTCGTGT TCAACAAGGT GGGCAATGCT 
ATCGCCCAGA GAATCCTGAA AGCCCACAGG GAAGGCCAGA GATATCGAGT GTACATCGTG 
ATACCACTGC TGCCGGGATT CGAAGGAGAC ATTTCCACAG GCGGGGGAAA TGCCCTACAG 
GCAATAATGC ACTTCAACTA CAGAACCATG TGCAGAGGGG AGAGCTCCAT CCTTGAACAG 
TTAAAGCCAG AGCTTGGTAA CAAGTGGATA AATTACATAT CATTTTGCGG TCTGAGAACA 
CATGCAGAGC TGGAAGGAAA CCTTGTCACT GAGCTCATCT ATGTCCACAG CAAGTTGTTA 
ATTGCTGATG ATAACACGGT TATCATAGGT TCTGCCAACA TCAATGACCG GAGCATGCTG 
GGAAAGCGAG ACAGCGAAAT GGCTGTCATA GTGCAGGACA CAGAGACAGT CCCGTCAGTG 
ATGGATGGAA AAGAATATCA AGCTGGCCGC TTCGCTCAAG GCCTTCGGCT GGAGTGCTTC 
AGGCTTGTCC TGGGTTATCT TTCTGACCCA AGTGAGGACA TTCAGGATCC TGTAAGTGAC 
AAATTCTTCA AGGAGATTTG GGTTTCAACG GCAGCTCGAA ATGCTACCAT ATATGATAAG 
GTCTTCCGGT GCCTTCCCAA TGACGAAGTG CACAATTTAA TCCAGTTGCG GGACTTTATA 
AACAAGCCTA TATTGGCAAA AGAGGACCGC CTTCGTGCCG AGGAAGAACT GAGGAAGATC 
CGTGGCTTCT TGGTGCAGTT CCCCTTCTAC TTCTTATCAG AAGAAAACCT GCTGCCTTCC 
GTTGGAACCA AGGAAGCCAT TGTGCCCATG GAGGTTTGGA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446126.1
CDS	488..3712
Translation	MSLKSEARVNTSTLQKIAADMSNLIENLDTRELHFEGEEVEYDASPGDPTAQEACIPFSSIYNTQGFKEPNIQIYLSGCPVKAQVLEVERFTSTSRMPSVNLYTIELTHGEFTWQVKRKFKHFQEFHRELLKYKAFIRIPIPTKRHTFRRQNVKEEPREMPSLPRSSENAIQEEQFFGRRKQLEDYLTKILKMPMYRNYHATTEFLDVSQLSFIHDLGPKGLEGMIMKRSGGHRIPGVNCCGHGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDKEFRIKVGKKETETKYGLRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTDFLKDHRFGSYAAVHENILAKWYVNAKGYFEDIANAMEGATEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRVTSGQSLGSLTAASVESMESLSLKDKHQSHKNEPVLKSVNDTDMKLKGIGKSRKFSKFSLYRQLHRRNLHNSDSISSVDSASSYFNHYRSHQNLIHGLKPHLKLFRPSSESEQGLTRHSADTGSIRSVQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIGSVVHGKAARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQATAHELRYQVPGAVHAKAQLLRSAADWSAGIKHHEESIHAAYTHVIENSKHYIYIENQFFISCADDKVVFNKVGNAIAQRILKAHREGQRYRVYIVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGESSILEQLKPELGNKWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFAQGLRLECFRLVLGYLSDPSEDIQDPVSDKFFKEIWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPILAKEDRLRAEEELRKIRGFLVQFPFYFLSEENLLPSVGTKEAIVPMEVWT

Target ORF information:

RefSeq Version	XM_017590637.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase D1 (Pld1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590637.1

ORF Insert Sequence:

ATGTCACTAA AAAGTGAGGC CCGGGTGAAC ACTTCTACAC TGCAGAAGAT CGCCGCAGAT 
ATGAGCAACC TGATAGAAAA TCTCGACACC CGGGAACTTC ACTTTGAGGG AGAGGAGGTG 
GAGTATGATG CATCTCCAGG AGACCCCACG GCTCAGGAAG CGTGCATCCC ATTCTCTTCT 
ATTTACAACA CTCAAGGATT TAAGGAGCCC AACATCCAGA TCTACCTCTC TGGCTGTCCT 
GTAAAAGCAC AAGTACTGGA AGTGGAGCGC TTCACGTCGA CCTCGAGGAT GCCAAGTGTC 
AACCTGTACA CCATCGAGTT AACACACGGG GAATTCACAT GGCAAGTTAA GAGGAAATTC 
AAGCACTTCC AAGAGTTTCA TAGAGAGCTC CTCAAGTACA AAGCCTTCAT CCGGATCCCC 
ATTCCCACCA AAAGACATAC GTTTAGAAGA CAAAATGTCA AAGAAGAGCC CAGAGAAATG 
CCCAGTCTGC CCCGTTCATC AGAAAATGCA ATCCAGGAGG AGCAGTTCTT TGGCAGGAGG 
AAGCAACTGG AAGATTACTT GACAAAAATA CTAAAAATGC CCATGTACAG AAACTATCAT 
GCCACGACAG AGTTCCTTGA TGTAAGCCAA CTGTCTTTTA TCCATGATTT GGGACCAAAG 
GGCCTGGAAG GAATGATCAT GAAAAGATCT GGGGGACACA GGATACCAGG TGTGAATTGC 
TGTGGCCATG GAAGAGCCTG CTACCGGTGG TCAAAGAGGT GGTTGATAGT GAAAGACTCT 
TTCCTGCTGT ATATGAAACC AGACAGCGGG GCGATCGCCT TCGTCCTTCT TGTAGACAAA 
GAATTCAGAA TCAAAGTGGG AAAGAAGGAG ACAGAAACGA AATATGGACT TCGAATTGAT 
AATCTTTCAA GGACACTGAT TTTAAAATGT AACAGCTACA GACATGCCCG GTGGTGGGGA 
GGCGCCATCG AGGAGTTCAT CCAGAAGCAC GGCACTGACT TCCTCAAAGA CCATCGCTTC 
GGCTCCTACG CTGCGGTCCA CGAGAACATT TTAGCTAAAT GGTACGTGAA CGCCAAAGGT 
TACTTTGAAG ATATCGCAAA CGCAATGGAA GGGGCAACGG AAGAGATTTT TATCACAGAT 
TGGTGGTTAA GTCCAGAAAT CTTCCTGAAG CGCCCAGTGG TGGAAGGCAA CCGCTGGAGG 
CTGGACTGCA TCCTCAAACG GAAAGCACAA CAAGGTGTGC GGATTTTCAT AATGCTTTAC 
AAAGAGGTGG AGCTAGCCCT CGGGATCAAC AGCGAGTACA CCAAGCGGAC TTTGATGCGG 
CTGCACCCCA ACATAAAGGT GATGAGACAC CCAGACCATG TGTCCTCCAG TGTGTATCTG 
TGGGCTCATC ACGAGAAGCT CGTCATTATC GACCAATCGG TGGCGTTTGT GGGTGGGATT 
GACCTGGCCT ATGGACGGTG GGATGACAAT GAGCACAGAC TCACAGATGT GGGCAGTGTC 
AAGCGGGTCA CCTCAGGACA GTCTCTGGGC TCTCTCACAG CAGCATCAGT AGAGTCTATG 
GAATCCTTAA GCCTCAAGGA CAAACATCAA TCTCATAAAA ACGAGCCCGT CTTAAAGAGT 
GTCAACGATA CCGACATGAA ACTGAAAGGC ATAGGGAAGT CCAGGAAATT CTCCAAATTT 
AGCCTCTATC GCCAACTTCA CCGCCGTAAT CTGCACAACT CGGACAGCAT TAGCAGCGTC 
GACAGCGCTT CCAGTTATTT TAACCACTAT AGAAGTCATC AGAATTTAAT CCATGGGTTA 
AAGCCCCACT TGAAACTCTT TCGCCCTTCC AGTGAGTCTG AGCAGGGCCT CACTAGGCAC 
AGTGCTGACA CCGGCTCCAT CCGAAGTGTG CAGACAGGAG TGGGAGAGCT CCATGGGGAG 
ACTAGGTTTT GGCATGGGAA GGATTACTGC AACTTTGTCT TCAAGGACTG GGTTCAACTG 
GACAAACCTT TTGCTGATTT CATCGACAGG TACTCCACCC CCCGGATGCC TTGGCATGAC 
ATTGGTTCTG TGGTTCACGG AAAGGCAGCT CGTGACGTGG CTCGACACTT CATCCAGCGC 
TGGAACTTCA CAAAGATTAT GAAGCCAAAA TATCGTTCCC TTTCTTATCC TTTCCTGCTC 
CCAAAGTCTC AAGCCACAGC GCATGAGCTG AGGTATCAGG TGCCAGGGGC CGTCCACGCC 
AAAGCACAGT TGCTCCGATC TGCTGCTGAC TGGTCTGCTG GTATAAAGCA CCACGAGGAA 
TCTATCCATG CTGCCTACAC CCATGTCATA GAGAACAGCA AGCACTACAT CTATATTGAA 
AACCAATTTT TCATAAGCTG CGCTGATGAT AAAGTCGTGT TCAACAAGGT GGGCAATGCT 
ATCGCCCAGA GAATCCTGAA AGCCCACAGG GAAGGCCAGA GATATCGAGT GTACATCGTG 
ATACCACTGC TGCCGGGATT CGAAGGAGAC ATTTCCACAG GCGGGGGAAA TGCCCTACAG 
GCAATAATGC ACTTCAACTA CAGAACCATG TGCAGAGGGG AGAGCTCCAT CCTTGAACAG 
TTAAAGCCAG AGCTTGGTAA CAAGTGGATA AATTACATAT CATTTTGCGG TCTGAGAACA 
CATGCAGAGC TGGAAGGAAA CCTTGTCACT GAGCTCATCT ATGTCCACAG CAAGTTGTTA 
ATTGCTGATG ATAACACGGT TATCATAGGT TCTGCCAACA TCAATGACCG GAGCATGCTG 
GGAAAGCGAG ACAGCGAAAT GGCTGTCATA GTGCAGGACA CAGAGACAGT CCCGTCAGTG 
ATGGATGGAA AAGAATATCA AGCTGGCCGC TTCGCTCAAG GCCTTCGGCT GGAGTGCTTC 
AGGCTTGTCC TGGGTTATCT TTCTGACCCA AGTGAGGACA TTCAGGATCC TGTAAGTGAC 
AAATTCTTCA AGGAGATTTG GGTTTCAACG GCAGCTCGAA ATGCTACCAT ATATGATAAG 
GTCTTCCGGT GCCTTCCCAA TGACGAAGTG CACAATTTAA TCCAGTTGCG GGACTTTATA 
AACAAGCCTA TATTGGCAAA AGAGGACCGC CTTCGTGCCG AGGAAGAACT GAGGAAGATC 
CGTGGCTTCT TGGTGCAGTT CCCCTTCTAC TTCTTATCAG AAGAAAACCT GCTGCCTTCC 
GTTGGAACCA AGGAAGCCAT TGTGCCCATG GAGGTTTGGA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pld1 ( XM_006232205.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232267.1 Rattus norvegicus phospholipase D1 (Pld1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa24574
Clone ID Related Accession (Same CDS sequence)	XM_006232206.3 , XM_017590637.1 , XM_006232205.3
Accession Version	XM_006232205.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholipase D1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232205.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTAA AAAGTGAGGC CCGGGTGAAC ACTTCTACAC TGCAGAAGAT CGCCGCAGAT 
ATGAGCAACC TGATAGAAAA TCTCGACACC CGGGAACTTC ACTTTGAGGG AGAGGAGGTG 
GAGTATGATG CATCTCCAGG AGACCCCACG GCTCAGGAAG CGTGCATCCC ATTCTCTTCT 
ATTTACAACA CTCAAGGATT TAAGGAGCCC AACATCCAGA TCTACCTCTC TGGCTGTCCT 
GTAAAAGCAC AAGTACTGGA AGTGGAGCGC TTCACGTCGA CCTCGAGGAT GCCAAGTGTC 
AACCTGTACA CCATCGAGTT AACACACGGG GAATTCACAT GGCAAGTTAA GAGGAAATTC 
AAGCACTTCC AAGAGTTTCA TAGAGAGCTC CTCAAGTACA AAGCCTTCAT CCGGATCCCC 
ATTCCCACCA AAAGACATAC GTTTAGAAGA CAAAATGTCA AAGAAGAGCC CAGAGAAATG 
CCCAGTCTGC CCCGTTCATC AGAAAATGCA ATCCAGGAGG AGCAGTTCTT TGGCAGGAGG 
AAGCAACTGG AAGATTACTT GACAAAAATA CTAAAAATGC CCATGTACAG AAACTATCAT 
GCCACGACAG AGTTCCTTGA TGTAAGCCAA CTGTCTTTTA TCCATGATTT GGGACCAAAG 
GGCCTGGAAG GAATGATCAT GAAAAGATCT GGGGGACACA GGATACCAGG TGTGAATTGC 
TGTGGCCATG GAAGAGCCTG CTACCGGTGG TCAAAGAGGT GGTTGATAGT GAAAGACTCT 
TTCCTGCTGT ATATGAAACC AGACAGCGGG GCGATCGCCT TCGTCCTTCT TGTAGACAAA 
GAATTCAGAA TCAAAGTGGG AAAGAAGGAG ACAGAAACGA AATATGGACT TCGAATTGAT 
AATCTTTCAA GGACACTGAT TTTAAAATGT AACAGCTACA GACATGCCCG GTGGTGGGGA 
GGCGCCATCG AGGAGTTCAT CCAGAAGCAC GGCACTGACT TCCTCAAAGA CCATCGCTTC 
GGCTCCTACG CTGCGGTCCA CGAGAACATT TTAGCTAAAT GGTACGTGAA CGCCAAAGGT 
TACTTTGAAG ATATCGCAAA CGCAATGGAA GGGGCAACGG AAGAGATTTT TATCACAGAT 
TGGTGGTTAA GTCCAGAAAT CTTCCTGAAG CGCCCAGTGG TGGAAGGCAA CCGCTGGAGG 
CTGGACTGCA TCCTCAAACG GAAAGCACAA CAAGGTGTGC GGATTTTCAT AATGCTTTAC 
AAAGAGGTGG AGCTAGCCCT CGGGATCAAC AGCGAGTACA CCAAGCGGAC TTTGATGCGG 
CTGCACCCCA ACATAAAGGT GATGAGACAC CCAGACCATG TGTCCTCCAG TGTGTATCTG 
TGGGCTCATC ACGAGAAGCT CGTCATTATC GACCAATCGG TGGCGTTTGT GGGTGGGATT 
GACCTGGCCT ATGGACGGTG GGATGACAAT GAGCACAGAC TCACAGATGT GGGCAGTGTC 
AAGCGGGTCA CCTCAGGACA GTCTCTGGGC TCTCTCACAG CAGCATCAGT AGAGTCTATG 
GAATCCTTAA GCCTCAAGGA CAAACATCAA TCTCATAAAA ACGAGCCCGT CTTAAAGAGT 
GTCAACGATA CCGACATGAA ACTGAAAGGC ATAGGGAAGT CCAGGAAATT CTCCAAATTT 
AGCCTCTATC GCCAACTTCA CCGCCGTAAT CTGCACAACT CGGACAGCAT TAGCAGCGTC 
GACAGCGCTT CCAGTTATTT TAACCACTAT AGAAGTCATC AGAATTTAAT CCATGGGTTA 
AAGCCCCACT TGAAACTCTT TCGCCCTTCC AGTGAGTCTG AGCAGGGCCT CACTAGGCAC 
AGTGCTGACA CCGGCTCCAT CCGAAGTGTG CAGACAGGAG TGGGAGAGCT CCATGGGGAG 
ACTAGGTTTT GGCATGGGAA GGATTACTGC AACTTTGTCT TCAAGGACTG GGTTCAACTG 
GACAAACCTT TTGCTGATTT CATCGACAGG TACTCCACCC CCCGGATGCC TTGGCATGAC 
ATTGGTTCTG TGGTTCACGG AAAGGCAGCT CGTGACGTGG CTCGACACTT CATCCAGCGC 
TGGAACTTCA CAAAGATTAT GAAGCCAAAA TATCGTTCCC TTTCTTATCC TTTCCTGCTC 
CCAAAGTCTC AAGCCACAGC GCATGAGCTG AGGTATCAGG TGCCAGGGGC CGTCCACGCC 
AAAGCACAGT TGCTCCGATC TGCTGCTGAC TGGTCTGCTG GTATAAAGCA CCACGAGGAA 
TCTATCCATG CTGCCTACAC CCATGTCATA GAGAACAGCA AGCACTACAT CTATATTGAA 
AACCAATTTT TCATAAGCTG CGCTGATGAT AAAGTCGTGT TCAACAAGGT GGGCAATGCT 
ATCGCCCAGA GAATCCTGAA AGCCCACAGG GAAGGCCAGA GATATCGAGT GTACATCGTG 
ATACCACTGC TGCCGGGATT CGAAGGAGAC ATTTCCACAG GCGGGGGAAA TGCCCTACAG 
GCAATAATGC ACTTCAACTA CAGAACCATG TGCAGAGGGG AGAGCTCCAT CCTTGAACAG 
TTAAAGCCAG AGCTTGGTAA CAAGTGGATA AATTACATAT CATTTTGCGG TCTGAGAACA 
CATGCAGAGC TGGAAGGAAA CCTTGTCACT GAGCTCATCT ATGTCCACAG CAAGTTGTTA 
ATTGCTGATG ATAACACGGT TATCATAGGT TCTGCCAACA TCAATGACCG GAGCATGCTG 
GGAAAGCGAG ACAGCGAAAT GGCTGTCATA GTGCAGGACA CAGAGACAGT CCCGTCAGTG 
ATGGATGGAA AAGAATATCA AGCTGGCCGC TTCGCTCAAG GCCTTCGGCT GGAGTGCTTC 
AGGCTTGTCC TGGGTTATCT TTCTGACCCA AGTGAGGACA TTCAGGATCC TGTAAGTGAC 
AAATTCTTCA AGGAGATTTG GGTTTCAACG GCAGCTCGAA ATGCTACCAT ATATGATAAG 
GTCTTCCGGT GCCTTCCCAA TGACGAAGTG CACAATTTAA TCCAGTTGCG GGACTTTATA 
AACAAGCCTA TATTGGCAAA AGAGGACCGC CTTCGTGCCG AGGAAGAACT GAGGAAGATC 
CGTGGCTTCT TGGTGCAGTT CCCCTTCTAC TTCTTATCAG AAGAAAACCT GCTGCCTTCC 
GTTGGAACCA AGGAAGCCAT TGTGCCCATG GAGGTTTGGA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232267.1
CDS	959..4183
Translation	MSLKSEARVNTSTLQKIAADMSNLIENLDTRELHFEGEEVEYDASPGDPTAQEACIPFSSIYNTQGFKEPNIQIYLSGCPVKAQVLEVERFTSTSRMPSVNLYTIELTHGEFTWQVKRKFKHFQEFHRELLKYKAFIRIPIPTKRHTFRRQNVKEEPREMPSLPRSSENAIQEEQFFGRRKQLEDYLTKILKMPMYRNYHATTEFLDVSQLSFIHDLGPKGLEGMIMKRSGGHRIPGVNCCGHGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDKEFRIKVGKKETETKYGLRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTDFLKDHRFGSYAAVHENILAKWYVNAKGYFEDIANAMEGATEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRVTSGQSLGSLTAASVESMESLSLKDKHQSHKNEPVLKSVNDTDMKLKGIGKSRKFSKFSLYRQLHRRNLHNSDSISSVDSASSYFNHYRSHQNLIHGLKPHLKLFRPSSESEQGLTRHSADTGSIRSVQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIGSVVHGKAARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQATAHELRYQVPGAVHAKAQLLRSAADWSAGIKHHEESIHAAYTHVIENSKHYIYIENQFFISCADDKVVFNKVGNAIAQRILKAHREGQRYRVYIVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGESSILEQLKPELGNKWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFAQGLRLECFRLVLGYLSDPSEDIQDPVSDKFFKEIWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPILAKEDRLRAEEELRKIRGFLVQFPFYFLSEENLLPSVGTKEAIVPMEVWT

Target ORF information:

RefSeq Version	XM_006232205.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase D1 (Pld1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232205.3

ORF Insert Sequence:

ATGTCACTAA AAAGTGAGGC CCGGGTGAAC ACTTCTACAC TGCAGAAGAT CGCCGCAGAT 
ATGAGCAACC TGATAGAAAA TCTCGACACC CGGGAACTTC ACTTTGAGGG AGAGGAGGTG 
GAGTATGATG CATCTCCAGG AGACCCCACG GCTCAGGAAG CGTGCATCCC ATTCTCTTCT 
ATTTACAACA CTCAAGGATT TAAGGAGCCC AACATCCAGA TCTACCTCTC TGGCTGTCCT 
GTAAAAGCAC AAGTACTGGA AGTGGAGCGC TTCACGTCGA CCTCGAGGAT GCCAAGTGTC 
AACCTGTACA CCATCGAGTT AACACACGGG GAATTCACAT GGCAAGTTAA GAGGAAATTC 
AAGCACTTCC AAGAGTTTCA TAGAGAGCTC CTCAAGTACA AAGCCTTCAT CCGGATCCCC 
ATTCCCACCA AAAGACATAC GTTTAGAAGA CAAAATGTCA AAGAAGAGCC CAGAGAAATG 
CCCAGTCTGC CCCGTTCATC AGAAAATGCA ATCCAGGAGG AGCAGTTCTT TGGCAGGAGG 
AAGCAACTGG AAGATTACTT GACAAAAATA CTAAAAATGC CCATGTACAG AAACTATCAT 
GCCACGACAG AGTTCCTTGA TGTAAGCCAA CTGTCTTTTA TCCATGATTT GGGACCAAAG 
GGCCTGGAAG GAATGATCAT GAAAAGATCT GGGGGACACA GGATACCAGG TGTGAATTGC 
TGTGGCCATG GAAGAGCCTG CTACCGGTGG TCAAAGAGGT GGTTGATAGT GAAAGACTCT 
TTCCTGCTGT ATATGAAACC AGACAGCGGG GCGATCGCCT TCGTCCTTCT TGTAGACAAA 
GAATTCAGAA TCAAAGTGGG AAAGAAGGAG ACAGAAACGA AATATGGACT TCGAATTGAT 
AATCTTTCAA GGACACTGAT TTTAAAATGT AACAGCTACA GACATGCCCG GTGGTGGGGA 
GGCGCCATCG AGGAGTTCAT CCAGAAGCAC GGCACTGACT TCCTCAAAGA CCATCGCTTC 
GGCTCCTACG CTGCGGTCCA CGAGAACATT TTAGCTAAAT GGTACGTGAA CGCCAAAGGT 
TACTTTGAAG ATATCGCAAA CGCAATGGAA GGGGCAACGG AAGAGATTTT TATCACAGAT 
TGGTGGTTAA GTCCAGAAAT CTTCCTGAAG CGCCCAGTGG TGGAAGGCAA CCGCTGGAGG 
CTGGACTGCA TCCTCAAACG GAAAGCACAA CAAGGTGTGC GGATTTTCAT AATGCTTTAC 
AAAGAGGTGG AGCTAGCCCT CGGGATCAAC AGCGAGTACA CCAAGCGGAC TTTGATGCGG 
CTGCACCCCA ACATAAAGGT GATGAGACAC CCAGACCATG TGTCCTCCAG TGTGTATCTG 
TGGGCTCATC ACGAGAAGCT CGTCATTATC GACCAATCGG TGGCGTTTGT GGGTGGGATT 
GACCTGGCCT ATGGACGGTG GGATGACAAT GAGCACAGAC TCACAGATGT GGGCAGTGTC 
AAGCGGGTCA CCTCAGGACA GTCTCTGGGC TCTCTCACAG CAGCATCAGT AGAGTCTATG 
GAATCCTTAA GCCTCAAGGA CAAACATCAA TCTCATAAAA ACGAGCCCGT CTTAAAGAGT 
GTCAACGATA CCGACATGAA ACTGAAAGGC ATAGGGAAGT CCAGGAAATT CTCCAAATTT 
AGCCTCTATC GCCAACTTCA CCGCCGTAAT CTGCACAACT CGGACAGCAT TAGCAGCGTC 
GACAGCGCTT CCAGTTATTT TAACCACTAT AGAAGTCATC AGAATTTAAT CCATGGGTTA 
AAGCCCCACT TGAAACTCTT TCGCCCTTCC AGTGAGTCTG AGCAGGGCCT CACTAGGCAC 
AGTGCTGACA CCGGCTCCAT CCGAAGTGTG CAGACAGGAG TGGGAGAGCT CCATGGGGAG 
ACTAGGTTTT GGCATGGGAA GGATTACTGC AACTTTGTCT TCAAGGACTG GGTTCAACTG 
GACAAACCTT TTGCTGATTT CATCGACAGG TACTCCACCC CCCGGATGCC TTGGCATGAC 
ATTGGTTCTG TGGTTCACGG AAAGGCAGCT CGTGACGTGG CTCGACACTT CATCCAGCGC 
TGGAACTTCA CAAAGATTAT GAAGCCAAAA TATCGTTCCC TTTCTTATCC TTTCCTGCTC 
CCAAAGTCTC AAGCCACAGC GCATGAGCTG AGGTATCAGG TGCCAGGGGC CGTCCACGCC 
AAAGCACAGT TGCTCCGATC TGCTGCTGAC TGGTCTGCTG GTATAAAGCA CCACGAGGAA 
TCTATCCATG CTGCCTACAC CCATGTCATA GAGAACAGCA AGCACTACAT CTATATTGAA 
AACCAATTTT TCATAAGCTG CGCTGATGAT AAAGTCGTGT TCAACAAGGT GGGCAATGCT 
ATCGCCCAGA GAATCCTGAA AGCCCACAGG GAAGGCCAGA GATATCGAGT GTACATCGTG 
ATACCACTGC TGCCGGGATT CGAAGGAGAC ATTTCCACAG GCGGGGGAAA TGCCCTACAG 
GCAATAATGC ACTTCAACTA CAGAACCATG TGCAGAGGGG AGAGCTCCAT CCTTGAACAG 
TTAAAGCCAG AGCTTGGTAA CAAGTGGATA AATTACATAT CATTTTGCGG TCTGAGAACA 
CATGCAGAGC TGGAAGGAAA CCTTGTCACT GAGCTCATCT ATGTCCACAG CAAGTTGTTA 
ATTGCTGATG ATAACACGGT TATCATAGGT TCTGCCAACA TCAATGACCG GAGCATGCTG 
GGAAAGCGAG ACAGCGAAAT GGCTGTCATA GTGCAGGACA CAGAGACAGT CCCGTCAGTG 
ATGGATGGAA AAGAATATCA AGCTGGCCGC TTCGCTCAAG GCCTTCGGCT GGAGTGCTTC 
AGGCTTGTCC TGGGTTATCT TTCTGACCCA AGTGAGGACA TTCAGGATCC TGTAAGTGAC 
AAATTCTTCA AGGAGATTTG GGTTTCAACG GCAGCTCGAA ATGCTACCAT ATATGATAAG 
GTCTTCCGGT GCCTTCCCAA TGACGAAGTG CACAATTTAA TCCAGTTGCG GGACTTTATA 
AACAAGCCTA TATTGGCAAA AGAGGACCGC CTTCGTGCCG AGGAAGAACT GAGGAAGATC 
CGTGGCTTCT TGGTGCAGTT CCCCTTCTAC TTCTTATCAG AAGAAAACCT GCTGCCTTCC 
GTTGGAACCA AGGAAGCCAT TGTGCCCATG GAGGTTTGGA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pld1 ( XM_008760891.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759113.1 Rattus norvegicus phospholipase D1 (Pld1), transcript variant X4, mRNA.

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CloneID	ORa43013
Clone ID Related Accession (Same CDS sequence)	XM_008760891.2
Accession Version	XM_008760891.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholipase D1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTAA AAAGTGAGGC CCGGGTGAAC ACTTCTACAC TGCAGAAGAT CGCCGCAGAT 
ATGAGCAACC TGATAGAAAA TCTCGACACC CGGGAACTTC ACTTTGAGGG AGAGGAGGTG 
GAGTATGATG CATCTCCAGG AGACCCCACG GCTCAGGAAG CGTGCATCCC ATTCTCTTCT 
ATTTACAACA CTCAAGGATT TAAGGAGCCC AACATCCAGA TCTACCTCTC TGGCTGTCCT 
GTAAAAGCAC AAGTACTGGA AGTGGAGCGC TTCACGTCGA CCTCGAGGAT GCCAAGTGTC 
AACCTGTACA CCATCGAGTT AACACACGGG GAATTCACAT GGCAAGTTAA GAGGAAATTC 
AAGCACTTCC AAGAGTTTCA TAGAGAGCTC CTCAAGTACA AAGCCTTCAT CCGGATCCCC 
ATTCCCACCA AAAGACATAC GTTTAGAAGA CAAAATGTCA AAGAAGAGCC CAGAGAAATG 
CCCAGTCTGC CCCGTTCATC AGAAAATGCA ATCCAGGAGG AGCAGTTCTT TGGCAGGAGG 
AAGCAACTGG AAGATTACTT GACAAAAATA CTAAAAATGC CCATGTACAG AAACTATCAT 
GCCACGACAG AGTTCCTTGA TGTAAGCCAA CTGTCTTTTA TCCATGATTT GGGACCAAAG 
GGCCTGGAAG GAATGATCAT GAAAAGATCT GGGGGACACA GGATACCAGG TGTGAATTGC 
TGTGGCCATG GAAGAGCCTG CTACCGGTGG TCAAAGAGGT GGTTGATAGT GAAAGACTCT 
TTCCTGCTGT ATATGAAACC AGACAGCGGG GCGATCGCCT TCGTCCTTCT TGTAGACAAA 
GAATTCAGAA TCAAAGTGGG AAAGAAGGAG ACAGAAACGA AATATGGACT TCGAATTGAT 
AATCTTTCAA GGACACTGAT TTTAAAATGT AACAGCTACA GACATGCCCG GTGGTGGGGA 
GGCGCCATCG AGGAGTTCAT CCAGAAGCAC GGCACTGACT TCCTCAAAGA CCATCGCTTC 
GGCTCCTACG CTGCGGTCCA CGAGAACATT TTAGCTAAAT GGTACGTGAA CGCCAAAGGT 
TACTTTGAAG ATATCGCAAA CGCAATGGAA GGGGCAACGG AAGAGATTTT TATCACAGAT 
TGGTGGTTAA GTCCAGAAAT CTTCCTGAAG CGCCCAGTGG TGGAAGGCAA CCGCTGGAGG 
CTGGACTGCA TCCTCAAACG GAAAGCACAA CAAGGTGTGC GGATTTTCAT AATGCTTTAC 
AAAGAGGTGG AGCTAGCCCT CGGGATCAAC AGCGAGTACA CCAAGCGGAC TTTGATGCGG 
CTGCACCCCA ACATAAAGGT GATGAGACAC CCAGACCATG TGTCCTCCAG TGTGTATCTG 
TGGGCTCATC ACGAGAAGCT CGTCATTATC GACCAATCGG TGGCGTTTGT GGGTGGGATT 
GACCTGGCCT ATGGACGGTG GGATGACAAT GAGCACAGAC TCACAGATGT GGGCAGTGTC 
AAGCGGGTCA CCTCAGGACA GTCTCTGGGC TCTCTCACAG CAGCATCAGT AGAGTCTATG 
GAATCCTTAA GCCTCAAGGA CAAACATCAA TCTCATAAAA ACGAGCCCGT CTTAAAGAGT 
GTCAACGATA CCGACATGAA ACTGAAAGGC ATAGGGAAGT CCAGGAAATT CTCCAAATTT 
AGCCTCTATC GCCAACTTCA CCGCCGTAAT CTGCACAACT CGGACAGCAT TAGCAGCGTC 
GACAGCGCTT CCAACACCGG CTCCATCCGA AGTGTGCAGA CAGGAGTGGG AGAGCTCCAT 
GGGGAGACTA GGTTTTGGCA TGGGAAGGAT TACTGCAACT TTGTCTTCAA GGACTGGGTT 
CAACTGGACA AACCTTTTGC TGATTTCATC GACAGGTACT CCACCCCCCG GATGCCTTGG 
CATGACATTG GTTCTGTGGT TCACGGAAAG GCAGCTCGTG ACGTGGCTCG ACACTTCATC 
CAGCGCTGGA ACTTCACAAA GATTATGAAG CCAAAATATC GTTCCCTTTC TTATCCTTTC 
CTGCTCCCAA AGTCTCAAGC CACAGCGCAT GAGCTGAGGT ATCAGGTGCC AGGGGCCGTC 
CACGCCAAAG CACAGTTGCT CCGATCTGCT GCTGACTGGT CTGCTGGTAT AAAGCACCAC 
GAGGAATCTA TCCATGCTGC CTACACCCAT GTCATAGAGA ACAGCAAGCA CTACATCTAT 
ATTGAAAACC AATTTTTCAT AAGCTGCGCT GATGATAAAG TCGTGTTCAA CAAGGTGGGC 
AATGCTATCG CCCAGAGAAT CCTGAAAGCC CACAGGGAAG GCCAGAGATA TCGAGTGTAC 
ATCGTGATAC CACTGCTGCC GGGATTCGAA GGAGACATTT CCACAGGCGG GGGAAATGCC 
CTACAGGCAA TAATGCACTT CAACTACAGA ACCATGTGCA GAGGGGAGAG CTCCATCCTT 
GAACAGTTAA AGCCAGAGCT TGGTAACAAG TGGATAAATT ACATATCATT TTGCGGTCTG 
AGAACACATG CAGAGCTGGA AGGAAACCTT GTCACTGAGC TCATCTATGT CCACAGCAAG 
TTGTTAATTG CTGATGATAA CACGGTTATC ATAGGTTCTG CCAACATCAA TGACCGGAGC 
ATGCTGGGAA AGCGAGACAG CGAAATGGCT GTCATAGTGC AGGACACAGA GACAGTCCCG 
TCAGTGATGG ATGGAAAAGA ATATCAAGCT GGCCGCTTCG CTCAAGGCCT TCGGCTGGAG 
TGCTTCAGGC TTGTCCTGGG TTATCTTTCT GACCCAAGTG AGGACATTCA GGATCCTGTA 
AGTGACAAAT TCTTCAAGGA GATTTGGGTT TCAACGGCAG CTCGAAATGC TACCATATAT 
GATAAGGTCT TCCGGTGCCT TCCCAATGAC GAAGTGCACA ATTTAATCCA GTTGCGGGAC 
TTTATAAACA AGCCTATATT GGCAAAAGAG GACCGCCTTC GTGCCGAGGA AGAACTGAGG 
AAGATCCGTG GCTTCTTGGT GCAGTTCCCC TTCTACTTCT TATCAGAAGA AAACCTGCTG 
CCTTCCGTTG GAACCAAGGA AGCCATTGTG CCCATGGAGG TTTGGACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759113.1
CDS	605..3715
Translation	MSLKSEARVNTSTLQKIAADMSNLIENLDTRELHFEGEEVEYDASPGDPTAQEACIPFSSIYNTQGFKEPNIQIYLSGCPVKAQVLEVERFTSTSRMPSVNLYTIELTHGEFTWQVKRKFKHFQEFHRELLKYKAFIRIPIPTKRHTFRRQNVKEEPREMPSLPRSSENAIQEEQFFGRRKQLEDYLTKILKMPMYRNYHATTEFLDVSQLSFIHDLGPKGLEGMIMKRSGGHRIPGVNCCGHGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDKEFRIKVGKKETETKYGLRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTDFLKDHRFGSYAAVHENILAKWYVNAKGYFEDIANAMEGATEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRVTSGQSLGSLTAASVESMESLSLKDKHQSHKNEPVLKSVNDTDMKLKGIGKSRKFSKFSLYRQLHRRNLHNSDSISSVDSASNTGSIRSVQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIGSVVHGKAARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQATAHELRYQVPGAVHAKAQLLRSAADWSAGIKHHEESIHAAYTHVIENSKHYIYIENQFFISCADDKVVFNKVGNAIAQRILKAHREGQRYRVYIVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGESSILEQLKPELGNKWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFAQGLRLECFRLVLGYLSDPSEDIQDPVSDKFFKEIWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPILAKEDRLRAEEELRKIRGFLVQFPFYFLSEENLLPSVGTKEAIVPMEVWT

Target ORF information:

RefSeq Version	XM_008760891.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase D1 (Pld1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760891.2

ORF Insert Sequence:

ATGTCACTAA AAAGTGAGGC CCGGGTGAAC ACTTCTACAC TGCAGAAGAT CGCCGCAGAT 
ATGAGCAACC TGATAGAAAA TCTCGACACC CGGGAACTTC ACTTTGAGGG AGAGGAGGTG 
GAGTATGATG CATCTCCAGG AGACCCCACG GCTCAGGAAG CGTGCATCCC ATTCTCTTCT 
ATTTACAACA CTCAAGGATT TAAGGAGCCC AACATCCAGA TCTACCTCTC TGGCTGTCCT 
GTAAAAGCAC AAGTACTGGA AGTGGAGCGC TTCACGTCGA CCTCGAGGAT GCCAAGTGTC 
AACCTGTACA CCATCGAGTT AACACACGGG GAATTCACAT GGCAAGTTAA GAGGAAATTC 
AAGCACTTCC AAGAGTTTCA TAGAGAGCTC CTCAAGTACA AAGCCTTCAT CCGGATCCCC 
ATTCCCACCA AAAGACATAC GTTTAGAAGA CAAAATGTCA AAGAAGAGCC CAGAGAAATG 
CCCAGTCTGC CCCGTTCATC AGAAAATGCA ATCCAGGAGG AGCAGTTCTT TGGCAGGAGG 
AAGCAACTGG AAGATTACTT GACAAAAATA CTAAAAATGC CCATGTACAG AAACTATCAT 
GCCACGACAG AGTTCCTTGA TGTAAGCCAA CTGTCTTTTA TCCATGATTT GGGACCAAAG 
GGCCTGGAAG GAATGATCAT GAAAAGATCT GGGGGACACA GGATACCAGG TGTGAATTGC 
TGTGGCCATG GAAGAGCCTG CTACCGGTGG TCAAAGAGGT GGTTGATAGT GAAAGACTCT 
TTCCTGCTGT ATATGAAACC AGACAGCGGG GCGATCGCCT TCGTCCTTCT TGTAGACAAA 
GAATTCAGAA TCAAAGTGGG AAAGAAGGAG ACAGAAACGA AATATGGACT TCGAATTGAT 
AATCTTTCAA GGACACTGAT TTTAAAATGT AACAGCTACA GACATGCCCG GTGGTGGGGA 
GGCGCCATCG AGGAGTTCAT CCAGAAGCAC GGCACTGACT TCCTCAAAGA CCATCGCTTC 
GGCTCCTACG CTGCGGTCCA CGAGAACATT TTAGCTAAAT GGTACGTGAA CGCCAAAGGT 
TACTTTGAAG ATATCGCAAA CGCAATGGAA GGGGCAACGG AAGAGATTTT TATCACAGAT 
TGGTGGTTAA GTCCAGAAAT CTTCCTGAAG CGCCCAGTGG TGGAAGGCAA CCGCTGGAGG 
CTGGACTGCA TCCTCAAACG GAAAGCACAA CAAGGTGTGC GGATTTTCAT AATGCTTTAC 
AAAGAGGTGG AGCTAGCCCT CGGGATCAAC AGCGAGTACA CCAAGCGGAC TTTGATGCGG 
CTGCACCCCA ACATAAAGGT GATGAGACAC CCAGACCATG TGTCCTCCAG TGTGTATCTG 
TGGGCTCATC ACGAGAAGCT CGTCATTATC GACCAATCGG TGGCGTTTGT GGGTGGGATT 
GACCTGGCCT ATGGACGGTG GGATGACAAT GAGCACAGAC TCACAGATGT GGGCAGTGTC 
AAGCGGGTCA CCTCAGGACA GTCTCTGGGC TCTCTCACAG CAGCATCAGT AGAGTCTATG 
GAATCCTTAA GCCTCAAGGA CAAACATCAA TCTCATAAAA ACGAGCCCGT CTTAAAGAGT 
GTCAACGATA CCGACATGAA ACTGAAAGGC ATAGGGAAGT CCAGGAAATT CTCCAAATTT 
AGCCTCTATC GCCAACTTCA CCGCCGTAAT CTGCACAACT CGGACAGCAT TAGCAGCGTC 
GACAGCGCTT CCAACACCGG CTCCATCCGA AGTGTGCAGA CAGGAGTGGG AGAGCTCCAT 
GGGGAGACTA GGTTTTGGCA TGGGAAGGAT TACTGCAACT TTGTCTTCAA GGACTGGGTT 
CAACTGGACA AACCTTTTGC TGATTTCATC GACAGGTACT CCACCCCCCG GATGCCTTGG 
CATGACATTG GTTCTGTGGT TCACGGAAAG GCAGCTCGTG ACGTGGCTCG ACACTTCATC 
CAGCGCTGGA ACTTCACAAA GATTATGAAG CCAAAATATC GTTCCCTTTC TTATCCTTTC 
CTGCTCCCAA AGTCTCAAGC CACAGCGCAT GAGCTGAGGT ATCAGGTGCC AGGGGCCGTC 
CACGCCAAAG CACAGTTGCT CCGATCTGCT GCTGACTGGT CTGCTGGTAT AAAGCACCAC 
GAGGAATCTA TCCATGCTGC CTACACCCAT GTCATAGAGA ACAGCAAGCA CTACATCTAT 
ATTGAAAACC AATTTTTCAT AAGCTGCGCT GATGATAAAG TCGTGTTCAA CAAGGTGGGC 
AATGCTATCG CCCAGAGAAT CCTGAAAGCC CACAGGGAAG GCCAGAGATA TCGAGTGTAC 
ATCGTGATAC CACTGCTGCC GGGATTCGAA GGAGACATTT CCACAGGCGG GGGAAATGCC 
CTACAGGCAA TAATGCACTT CAACTACAGA ACCATGTGCA GAGGGGAGAG CTCCATCCTT 
GAACAGTTAA AGCCAGAGCT TGGTAACAAG TGGATAAATT ACATATCATT TTGCGGTCTG 
AGAACACATG CAGAGCTGGA AGGAAACCTT GTCACTGAGC TCATCTATGT CCACAGCAAG 
TTGTTAATTG CTGATGATAA CACGGTTATC ATAGGTTCTG CCAACATCAA TGACCGGAGC 
ATGCTGGGAA AGCGAGACAG CGAAATGGCT GTCATAGTGC AGGACACAGA GACAGTCCCG 
TCAGTGATGG ATGGAAAAGA ATATCAAGCT GGCCGCTTCG CTCAAGGCCT TCGGCTGGAG 
TGCTTCAGGC TTGTCCTGGG TTATCTTTCT GACCCAAGTG AGGACATTCA GGATCCTGTA 
AGTGACAAAT TCTTCAAGGA GATTTGGGTT TCAACGGCAG CTCGAAATGC TACCATATAT 
GATAAGGTCT TCCGGTGCCT TCCCAATGAC GAAGTGCACA ATTTAATCCA GTTGCGGGAC 
TTTATAAACA AGCCTATATT GGCAAAAGAG GACCGCCTTC GTGCCGAGGA AGAACTGAGG 
AAGATCCGTG GCTTCTTGGT GCAGTTCCCC TTCTACTTCT TATCAGAAGA AAACCTGCTG 
CCTTCCGTTG GAACCAAGGA AGCCATTGTG CCCATGGAGG TTTGGACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of PLD1 activity causes ER stress via regulation of COPII vesicle formation.
Biochemical and biophysical research communications490(3)895-900(2017 08)
Nakagawa H,Hazama K,Ishida K,Komori M,Nishimura K,Matsuo S

PLD1 promotes dendritic spine development by inhibiting ADAM10-mediated N-cadherin cleavage.
Scientific reports7(1)6035(2017 Jul)
Luo LD,Li G,Wang Y

Simultaneous high expression of PLD1 and Sp1 predicts a poor prognosis for pancreatic ductal adenocarcinoma patients.
Oncotarget7(48)78557-78565(2016 Nov)
Hu J,Hu H,Hang JJ,Yang HY,Wang ZY,Wang L,Chen DH,Wang LW

Phospholipase D1 increases Bcl-2 expression during neuronal differentiation of rat neural stem cells.
Molecular neurobiology51(3)1089-102(2015)
Park SY,Ma W,Yoon SN,Kang MJ,Han JS

The role of phospholipase D1 in liver fibrosis induced by dimethylnitrosamine in vivo.
Digestive diseases and sciences59(8)1779-88(2014 Aug)
Zhu X,Liu R,Kuang D,Liu J,Shi X,Zhang T,Zeng Y,Sun X,Zhang Y,Yang W