Acat1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acat1
Entrez Gene ID	25014
Full Name	acetyl-CoA acetyltransferase 1
Synonyms	RATACAL
General protein information	Preferred Names acetyl-CoA acetyltransferase 1 Names acetyl-CoA acetyltransferase, mitochondrial acetoacetyl-CoA thiolase acetyl-Co A acetyltransferase 1 mitochondrial acetyl-coenzyme A acetyltransferase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	catalyzes the conversion of 2 acetyl-CoA molecules to acetoacetyl-CoA plus CoA [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017075.2	NP_058771.2	acetyl-CoA acetyltransferase, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ACAT1	XP_508738.2
Gallus gallus (chicken)	ACAT1	NP_001264708.1
Drosophila melanogaster (fruit fly)	CG10932	NP_572414.1
Kluyveromyces lactis	KLLA0D12056g	XP_453599.1
Schizosaccharomyces pombe (fission yeast)	erg10	NP_596686.1
Magnaporthe oryzae (rice blast fungus)	MGG_13499	XP_003716277.1
Oryza sativa (rice)	Os09g0252100	NP_001062685.1
	ACAT1	XP_001103928.1
Caenorhabditis elegans (roundworm)	T02G5.4	NP_495461.2
Neurospora crassa	NCU02571	XP_965702.2
Arabidopsis thaliana (thale cress)	AT5G47720	NP_001032028.1
Homo sapiens (human)	ACAT1	NP_000010.1
Mus musculus (house mouse)	Acat1	NP_659033.1
Rattus norvegicus (Norway rat)	Acat1	NP_058771.2
Saccharomyces cerevisiae (baker's yeast)	ERG10	NP_015297.1
Canis lupus familiaris (dog)	ACAT1	XP_546539.2
Danio rerio (zebrafish)	acat1	NP_001003746.1
Caenorhabditis elegans (roundworm)	kat-1	NP_495455.2
Eremothecium gossypii	AGOS_ADR165C	NP_984262.1
Arabidopsis thaliana (thale cress)	ACAT2	NP_568694.2
Xenopus tropicalis (tropical clawed frog)	acat1	NP_001025572.1
Bos taurus (cattle)	ACAT1	NP_001039540.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011329	XP_309320.4

Gene Ontology
Bibliography

Related articles in PubMed

Neurobehavioral Deficits in a Rat Model of Recurrent Neonatal Seizures Are Prevented by a Ketogenic Diet and Correlate with Hippocampal Zinc/Lipid Transporter Signals.
Tian T, Ni H, Sun BL
Biological trace element research167(2)251-8(2015 Oct)

Niacin improves renal lipid metabolism and slows progression in chronic kidney disease.
Cho KH, Kim HJ, Kamanna VS, Vaziri ND
Biochimica et biophysica acta1800(1)6-15(2010 Jan)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Sterol regulatory element-binding proteins, liver X receptor, ABCA1 transporter, CD36, scavenger receptors A1 and B1 in nephrotic kidney.
Kim HJ, Vaziri ND
American journal of nephrology29(6)607-14(2009)

Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function.
Haapalainen AM, Meril?inen G, Piril? PL, Kondo N, Fukao T, Wierenga RK
Biochemistry46(14)4305-21(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

rats treated with KD (EXP2) showed a significant down-regulated expression of ZnT-3, MT-3, ApoE, clusterin, and ACAT-1 in hippocampus
Title: Neurobehavioral Deficits in a Rat Model of Recurrent Neonatal Seizures Are Prevented by a Ketogenic Diet and Correlate with Hippocampal Zinc/Lipid Transporter Signals.

The following Acat1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acat1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12154	NM_017075.2 Latest version!	Rattus norvegicus acetyl-CoA acetyltransferase 1 (Acat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acat1 ( NM_017075.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058771.2 Rattus norvegicus acetyl-CoA acetyltransferase 1 (Acat1), mRNA.

Price & Availability↑

CloneID	ORa12154
Clone ID Related Accession (Same CDS sequence)	NM_017075.2
Accession Version	NM_017075.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	acetyl-CoA acetyltransferase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM065829.1, FM071064.1, FM098848.1 and AABR07070208.1. On Aug 16, 2012 this sequence version replaced NM_017075.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ228108.1, FQ217320.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689600 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTGCCC TGGCGGTTCT ACACGGCGTC GTCCGCAGGC CTCTGCTCCG CGGGCTGCTG 
CAGGAAGTAA GATGCCTGGG ACGAAGTTAT GCATCCAAAC CCACTTTGAA TGATGTGGTT 
ATAGTGAGTG CTACACGAAC TCCCATTGGA TCCTTCCTGG GCAGCCTTGC CTCTCAGCCA 
GCCACCAAGC TTGGTACTAT TGCAATTCAG GGAGCCATTG AAAAGGCAGG GATTCCAAAA 
GAAGAAGTGA AGGAGGTCTA CATGGGCAAC GTCATCCAAG GGGGAGAAGG ACAGGCCCCG 
ACCAGGCAAG CTACACTGGG TGCAGGTCTA CCCATTGCCA CTCCGTGCAC CACAGTAAAC 
AAGGTGTGTG CCTCAGGAAT GAAAGCCATC ATGATGGCCT CTCAAAGTCT TATGTGTGGA 
CACCAGGATG TGATGGTGGC AGGCGGAATG GAGAGCATGT CAAATGTCCC GTACGTAATG 
AGCAGAGGAG CAACACCATA CGGTGGGGTA AAACTTGAAG ACCTGATTGT GAAAGACGGG 
CTAACCGATG TCTACAATAA AATTCATATG GGCAATTGTG CTGAGAACAC CGCGAAGAAG 
CTGAGTATCT CGCGGGAGGA GCAGGATAAG TACGCCATCG GCTCTTACAC CCGAAGTAAA 
GAGGCGTGGG ATGCAGGGAA GTTTGCAAAT GAGATTACGC CCATCACCAT CTCAGTGAAA 
GGTAAACCAG ACGTGGTGGT GAAGGAAGAT GAAGAGTACA AGCGAGTTGA CTTCAGTAAA 
GTGCCAAAGC TCAAGACAGT GTTCCAGAAA GAAAACGGCA CAGTAACAGC TGCTAACGCC 
AGCACACTGA ACGATGGAGC AGCTGCTGTG GTTCTCATGA CTGCAGAGGC AGCCCAGAGG 
CTCAAGGTTA AGCCACTGGC ACGAATCGCA GCATTTGCTG ATGCTGCTGT AGACCCCATT 
GATTTCCCAC TCGCACCTGC ATATGCTGTA CCTAAGGTTC TTAAATATGC AGGACTGAAA 
AAAGAAGACA TTGCCATGTG GGAAGTAAAT GAAGCATTCA GTGTGGTTGT ACTAGCCAAC 
ATTAAAATGC TGGAGATTGA CCCTCAAAAA GTAAATGTCC ATGGAGGAGC TGTTTCTCTG 
GGCCATCCAA TCGGGATGTC TGGAGCTCGG ATTGTTGTTC ACTTGGCTCA TGCCTTGAAG 
CAAGGAGAAT TCGGTCTGGC TAGTATTTGC AATGGAGGAG GAGGGGCTTC CGCCGTGCTG 
ATTGAGAAGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058771.2
CDS	89..1363
Translation	MAALAVLHGVVRRPLLRGLLQEVRCLGRSYASKPTLNDVVIVSATRTPIGSFLGSLASQPATKLGTIAIQGAIEKAGIPKEEVKEVYMGNVIQGGEGQAPTRQATLGAGLPIATPCTTVNKVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVPYVMSRGATPYGGVKLEDLIVKDGLTDVYNKIHMGNCAENTAKKLSISREEQDKYAIGSYTRSKEAWDAGKFANEITPITISVKGKPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAAVVLMTAEAAQRLKVKPLARIAAFADAAVDPIDFPLAPAYAVPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKMLEIDPQKVNVHGGAVSLGHPIGMSGARIVVHLAHALKQGEFGLASICNGGGGASAVLIEKL

Target ORF information:

RefSeq Version	NM_017075.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acetyl-CoA acetyltransferase 1 (Acat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017075.2

ORF Insert Sequence:

ATGGCTGCCC TGGCGGTTCT ACACGGCGTC GTCCGCAGGC CTCTGCTCCG CGGGCTGCTG 
CAGGAAGTAA GATGCCTGGG ACGAAGTTAT GCATCCAAAC CCACTTTGAA TGATGTGGTT 
ATAGTGAGTG CTACACGAAC TCCCATTGGA TCCTTCCTGG GCAGCCTTGC CTCTCAGCCA 
GCCACCAAGC TTGGTACTAT TGCAATTCAG GGAGCCATTG AAAAGGCAGG GATTCCAAAA 
GAAGAAGTGA AGGAGGTCTA CATGGGCAAC GTCATCCAAG GGGGAGAAGG ACAGGCCCCG 
ACCAGGCAAG CTACACTGGG TGCAGGTCTA CCCATTGCCA CTCCGTGCAC CACAGTAAAC 
AAGGTGTGTG CCTCAGGAAT GAAAGCCATC ATGATGGCCT CTCAAAGTCT TATGTGTGGA 
CACCAGGATG TGATGGTGGC AGGCGGAATG GAGAGCATGT CAAATGTCCC GTACGTAATG 
AGCAGAGGAG CAACACCATA CGGTGGGGTA AAACTTGAAG ACCTGATTGT GAAAGACGGG 
CTAACCGATG TCTACAATAA AATTCATATG GGCAATTGTG CTGAGAACAC CGCGAAGAAG 
CTGAGTATCT CGCGGGAGGA GCAGGATAAG TACGCCATCG GCTCTTACAC CCGAAGTAAA 
GAGGCGTGGG ATGCAGGGAA GTTTGCAAAT GAGATTACGC CCATCACCAT CTCAGTGAAA 
GGTAAACCAG ACGTGGTGGT GAAGGAAGAT GAAGAGTACA AGCGAGTTGA CTTCAGTAAA 
GTGCCAAAGC TCAAGACAGT GTTCCAGAAA GAAAACGGCA CAGTAACAGC TGCTAACGCC 
AGCACACTGA ACGATGGAGC AGCTGCTGTG GTTCTCATGA CTGCAGAGGC AGCCCAGAGG 
CTCAAGGTTA AGCCACTGGC ACGAATCGCA GCATTTGCTG ATGCTGCTGT AGACCCCATT 
GATTTCCCAC TCGCACCTGC ATATGCTGTA CCTAAGGTTC TTAAATATGC AGGACTGAAA 
AAAGAAGACA TTGCCATGTG GGAAGTAAAT GAAGCATTCA GTGTGGTTGT ACTAGCCAAC 
ATTAAAATGC TGGAGATTGA CCCTCAAAAA GTAAATGTCC ATGGAGGAGC TGTTTCTCTG 
GGCCATCCAA TCGGGATGTC TGGAGCTCGG ATTGTTGTTC ACTTGGCTCA TGCCTTGAAG 
CAAGGAGAAT TCGGTCTGGC TAGTATTTGC AATGGAGGAG GAGGGGCTTC CGCCGTGCTG 
ATTGAGAAGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neurobehavioral Deficits in a Rat Model of Recurrent Neonatal Seizures Are Prevented by a Ketogenic Diet and Correlate with Hippocampal Zinc/Lipid Transporter Signals.
Biological trace element research167(2)251-8(2015 Oct)
Tian T,Ni H,Sun BL

Niacin improves renal lipid metabolism and slows progression in chronic kidney disease.
Biochimica et biophysica acta1800(1)6-15(2010 Jan)
Cho KH,Kim HJ,Kamanna VS,Vaziri ND

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Sterol regulatory element-binding proteins, liver X receptor, ABCA1 transporter, CD36, scavenger receptors A1 and B1 in nephrotic kidney.
American journal of nephrology29(6)607-14(2009)
Kim HJ,Vaziri ND

Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function.
Biochemistry46(14)4305-21(2007 Apr)
Haapalainen AM,Meril?inen G,Piril? PL,Kondo N,Fukao T,Wierenga RK