Prkar2b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkar2b
Entrez Gene ID	24679
Full Name	protein kinase cAMP-dependent type 2 regulatory subunit beta
Synonyms	RATDNA
General protein information	Preferred Names protein kinase cAMP-dependent type 2 regulatory subunit beta Names cAMP-dependent protein kinase type II-beta regulatory subunit Type II beta regulatory subunit of cAMP-dependent protein kinase protein kinase, cAMP dependent regulatory, type II beta protein kinase, cAMP-dependent, regulatory subunit type II beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q16
Summary	acts as a regulatory subunit of type II cAMP-dependent protein kinase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594037.1	XP_017449526.1	cAMP-dependent protein kinase type II-beta regulatory subunit isoform X1
NM_001030020.1	NP_001025191.1	cAMP-dependent protein kinase type II-beta regulatory subunit

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkar2b	NP_035288.2
Bos taurus (cattle)	PRKAR2B	NP_777074.1
Rattus norvegicus (Norway rat)	Prkar2b	NP_001025191.1
Xenopus tropicalis (tropical clawed frog)	prkar2b	XP_002933139.2
Homo sapiens (human)	PRKAR2B	NP_002727.2
	PRKAR2B	XP_001089109.2
Gallus gallus (chicken)	PRKAR2B	XP_001232697.3
Pan troglodytes (chimpanzee)	PRKAR2B	XP_001148361.2
Danio rerio (zebrafish)	LOC100331387	XP_002666815.3
Canis lupus familiaris (dog)	PRKAR2B	XP_858066.2

Gene Ontology
Bibliography

Related articles in PubMed

Single Turnover Autophosphorylation Cycle of the PKA RII? Holoenzyme.
Zhang P, Knape MJ, Ahuja LG, Keshwani MM, King CC, Sastri M, Herberg FW, Taylor SS
PLoS biology13(7)e1002192(2015 Jul)

The roles of the RII? linker and N-terminal cyclic nucleotide-binding domain in determining the unique structures of the type II? protein kinase A: a small angle x-ray and neutron scattering study.
Blumenthal DK, Copps J, Smith-Nguyen EV, Zhang P, Heller WT, Taylor SS
The Journal of biological chemistry289(41)28505-12(2014 Oct)

LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity.
Parisiadou L, Yu J, Sgobio C, Xie C, Liu G, Sun L, Gu XL, Lin X, Crowley NA, Lovinger DM, Cai H
Nature neuroscience17(3)367-76(2014 Mar)

Structure and allostery of the PKA RII? tetrameric holoenzyme.
Zhang P, Smith-Nguyen EV, Keshwani MM, Deal MS, Kornev AP, Taylor SS
Science (New York, N.Y.)335(6069)712-6(2012 Feb)

Protein kinase A acts at the basal body of the primary cilium to prevent Gli2 activation and ventralization of the mouse neural tube.
Tuson M, He M, Anderson KV
Development (Cambridge, England)138(22)4921-30(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Release of pRIIbeta in the presence of cAMP is reduced by calcium, whereas autophosphorylation at the phosphorylation site inhibits holoenzyme reassociation with the catalytic subunit.
Title: Single Turnover Autophosphorylation Cycle of the PKA RII? Holoenzyme.

The unique structural properties of RiIbeta do not require the C-terminal cAMP-binding domain.
Title: The roles of the RII? linker and N-terminal cyclic nucleotide-binding domain in determining the unique structures of the type II? protein kinase A: a small angle x-ray and neutron scattering study.

study describes the 2.3 angstrom structure of full-length tetrameric RIIbeta(2):C(2) holoenzyme
Title: Structure and allostery of the PKA RII? tetrameric holoenzyme.

The novel orientation of the linker peptide provides the first clues as to how this region contributes to the unique organization of the RIIbeta holoenzyme.
Title: Novel isoform-specific interfaces revealed by PKA RIIbeta holoenzyme structures.

InsP3R1 forms a complex with AKAP450 and PKAIIbeta, localized in the Golgi apparatus of cerebellar granule cells.
Title: Ins(1,4,5)P3 receptor type 1 associates with AKAP9 (AKAP450 variant) and protein kinase A type IIbeta in the Golgi apparatus in cerebellar granule cells.

The following Prkar2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkar2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12924	NM_001030020.1 Latest version!	Rattus norvegicus protein kinase cAMP-dependent type II regulatory subunit beta (Prkar2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa61553	XM_017594037.1 Latest version!	Rattus norvegicus protein kinase cAMP-dependent type 2 regulatory subunit beta (Prkar2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkar2b ( NM_001030020.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001025191.1 Rattus norvegicus protein kinase cAMP-dependent type II regulatory subunit beta (Prkar2b), mRNA.

Price & Availability↑

CloneID	ORa12924
Clone ID Related Accession (Same CDS sequence)	NM_001030020.1
Accession Version	NM_001030020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase type II-beta regulatory subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB814252.1, M12492.1, CX569141.1 and BI298837.1. On or before Oct 11, 2007 this sequence version replaced XM_343046.3, XM_001073478.1, XM_001073542.1, XM_001073576.1, XM_001073507.1, XM_001073451.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M12492.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCATCG AGATCCCCGC GGGGCTCACG GAGCTGCTGC AGGGCTTCAC GGTGGAGGTG 
CTGAGGCACC AGCCCGCCGA CCTGCTGGAG TTCGCGCTGC AGCACTTCAC GCGGCTGCAG 
CAGGAGAACG AGCGCAAGGG CGCCGCGCGC TTCGGCCATG AGGGCAGGAC CTGGGGGGAC 
GCGGGCGCAG CCGCCGGGGG CGGAACCCCC AGTAAGGGTG TCAACTTCGC GGAGGAGCCC 
ATGCGCTCCG ATTCCGAGAA CGGCGAAGAG GAGGAGGCCG CGGAAGCAGG GGCGTTCAAC 
GCTCCAGTTA TAAACCGGTT CACAAGGCGT GCCTCGGTAT GTGCAGAAGC TTATAATCCT 
GATGAAGAAG AAGATGATGC AGAGTCCAGG ATAATACATC CCAAAACTGA CGATCAAAGA 
AACAGATTGC AAGAAGCCTG CAAAGACATC CTGCTGTTTA AGAACCTGGA TCCAGAACAG 
ATGTCTCAAG TATTAGATGC CATGTTTGAA AAATTGGTCA AAGAAGGGGA ACACGTAATC 
GATCAAGGTG ATGATGGTGA CAACTTTTAC GTCATCGACA GAGGAACATT TGATATTTAT 
GTAAAATGTG ATGGCGTTGG AAGATGCGTT GGTAACTATG ACAATCGTGG GAGTTTTGGA 
GAACTGGCCT TAATGTACAA TACACCCAGA GCAGCTACAA TCACTGCTAC CTCTCCTGGT 
GCTCTGTGGG GTTTGGACAG GGTGACCTTC AGGAGAATAA TAGTAAAAAA CAATGCCAAA 
AAGAGGAAGA TGTACGAGAG CTTTATTGAG TCACTGCCAT TCCTCAAGTC TCTGGAGGTT 
TCTGAACGCC TGAAGGTGGT AGATGTGATT GGCACCAAAG TTTACAACGA TGGAGAACAG 
ATCATTGCTC AGGGAGACTC GGCGGATTCG TTCTTCATTG TAGAATCTGG AGAAGTGAGA 
ATTACTATGA AGAGAAAGGG TAAATCAGAC ATCGAAGAGA ACGGTGCTGT GGAAATCGCT 
CGGTGTCTCC GGGGACAGTA TTTTGGAGAG CTTGCCCTGG TCACTAACAA GCCAAGAGCA 
GCATCTGCAC ACGCCATTGG GACTGTCAAA TGCTTAGCCA TGGATGTGCA AGCATTTGAG 
AGGCTTCTGG GACCTTGCAT GGAAATTATG AAGAGGAATA TCGCCACCTA TGAGGAGCAA 
TTAGTTGCAC TGTTTGGGAC AAACATGGAT ATTGTTGAGC CCACTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025191.1
CDS	6..1256
Translation	MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGAARFGHEGRTWGDAGAAAGGGTPSKGVNFAEEPMRSDSENGEEEEAAEAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVRITMKRKGKSDIEENGAVEIARCLRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

Target ORF information:

RefSeq Version	NM_001030020.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cAMP-dependent type II regulatory subunit beta (Prkar2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030020.1

ORF Insert Sequence:

ATGAGCATCG AGATCCCCGC GGGGCTCACG GAGCTGCTGC AGGGCTTCAC GGTGGAGGTG 
CTGAGGCACC AGCCCGCCGA CCTGCTGGAG TTCGCGCTGC AGCACTTCAC GCGGCTGCAG 
CAGGAGAACG AGCGCAAGGG CGCCGCGCGC TTCGGCCATG AGGGCAGGAC CTGGGGGGAC 
GCGGGCGCAG CCGCCGGGGG CGGAACCCCC AGTAAGGGTG TCAACTTCGC GGAGGAGCCC 
ATGCGCTCCG ATTCCGAGAA CGGCGAAGAG GAGGAGGCCG CGGAAGCAGG GGCGTTCAAC 
GCTCCAGTTA TAAACCGGTT CACAAGGCGT GCCTCGGTAT GTGCAGAAGC TTATAATCCT 
GATGAAGAAG AAGATGATGC AGAGTCCAGG ATAATACATC CCAAAACTGA CGATCAAAGA 
AACAGATTGC AAGAAGCCTG CAAAGACATC CTGCTGTTTA AGAACCTGGA TCCAGAACAG 
ATGTCTCAAG TATTAGATGC CATGTTTGAA AAATTGGTCA AAGAAGGGGA ACACGTAATC 
GATCAAGGTG ATGATGGTGA CAACTTTTAC GTCATCGACA GAGGAACATT TGATATTTAT 
GTAAAATGTG ATGGCGTTGG AAGATGCGTT GGTAACTATG ACAATCGTGG GAGTTTTGGA 
GAACTGGCCT TAATGTACAA TACACCCAGA GCAGCTACAA TCACTGCTAC CTCTCCTGGT 
GCTCTGTGGG GTTTGGACAG GGTGACCTTC AGGAGAATAA TAGTAAAAAA CAATGCCAAA 
AAGAGGAAGA TGTACGAGAG CTTTATTGAG TCACTGCCAT TCCTCAAGTC TCTGGAGGTT 
TCTGAACGCC TGAAGGTGGT AGATGTGATT GGCACCAAAG TTTACAACGA TGGAGAACAG 
ATCATTGCTC AGGGAGACTC GGCGGATTCG TTCTTCATTG TAGAATCTGG AGAAGTGAGA 
ATTACTATGA AGAGAAAGGG TAAATCAGAC ATCGAAGAGA ACGGTGCTGT GGAAATCGCT 
CGGTGTCTCC GGGGACAGTA TTTTGGAGAG CTTGCCCTGG TCACTAACAA GCCAAGAGCA 
GCATCTGCAC ACGCCATTGG GACTGTCAAA TGCTTAGCCA TGGATGTGCA AGCATTTGAG 
AGGCTTCTGG GACCTTGCAT GGAAATTATG AAGAGGAATA TCGCCACCTA TGAGGAGCAA 
TTAGTTGCAC TGTTTGGGAC AAACATGGAT ATTGTTGAGC CCACTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkar2b ( XM_017594037.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449526.1 Rattus norvegicus protein kinase cAMP-dependent type 2 regulatory subunit beta (Prkar2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61553
Clone ID Related Accession (Same CDS sequence)	XM_017594037.1
Accession Version	XM_017594037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase type II-beta regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCCAG ATCTGCAATC TTTGGTTGGA TTCATCAGTG CTGTCTCAAG CCATTGTCCT 
TCTCCAGTTA TAAACCGGTT CACAAGGCGT GCCTCGGTAT GTGCAGAAGC TTATAATCCT 
GATGAAGAAG AAGATGATGC AGAGTCCAGG ATAATACATC CCAAAACTGA CGATCAAAGA 
AACAGATTGC AAGAAGCCTG CAAAGACATC CTGCTGTTTA AGAACCTGGA TCCAGAACAG 
ATGTCTCAAG TATTAGATGC CATGTTTGAA AAATTGGTCA AAGAAGGGGA ACACGTAATC 
GATCAAGGTG ATGATGGTGA CAACTTTTAC GTCATCGACA GAGGAACATT TGATATTTAT 
GTAAAATGTG ATGGCGTTGG AAGATGCGTT GGTAACTATG ACAATCGTGG GAGTTTTGGA 
GAACTGGCCT TAATGTACAA TACACCCAGA GCAGCTACAA TCACTGCTAC CTCTCCTGGT 
GCTCTGTGGG GTTTGGACAG GGTGACCTTC AGGAGAATAA TAGTAAAAAA CAATGCCAAA 
AAGAGGAAGA TGTACGAGAG CTTTATTGAG TCACTGCCAT TCCTCAAGTC TCTGGAGGTT 
TCTGAACGCC TGAAGGTGGT AGATGTGATT GGCACCAAAG TTTACAACGA TGGAGAACAG 
ATCATTGCTC AGGGAGACTC GGCGGATTCG TTCTTCATTG TAGAATCTGG AGAAGTGAGA 
ATTACTATGA AGAGAAAGGG TAAATCAGAC ATCGAAGAGA ACGGTGCTGT GGAAATCGCT 
CGGTGTCTCC GGGGACAGTA TTTTGGAGAG CTTGCCCTGG TCACTAACAA GCCAAGAGCA 
GCATCTGCAC ACGCCATTGG GACTGTCAAA TGCTTAGCCA TGGATGTGCA AGCATTTGAG 
AGGCTTCTGG GACCTTGCAT GGAAATTATG AAGAGGAATA TCGCCACCTA TGAGGAGCAA 
TTAGTTGCAC TGTTTGGGAC AAACATGGAT ATTGTTGAGC CCACTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449526.1
CDS	369..1379
Translation	MLPDLQSLVGFISAVSSHCPSPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVRITMKRKGKSDIEENGAVEIARCLRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

Target ORF information:

RefSeq Version	XM_017594037.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cAMP-dependent type 2 regulatory subunit beta (Prkar2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594037.1

ORF Insert Sequence:

ATGCTCCCAG ATCTGCAATC TTTGGTTGGA TTCATCAGTG CTGTCTCAAG CCATTGTCCT 
TCTCCAGTTA TAAACCGGTT CACAAGGCGT GCCTCGGTAT GTGCAGAAGC TTATAATCCT 
GATGAAGAAG AAGATGATGC AGAGTCCAGG ATAATACATC CCAAAACTGA CGATCAAAGA 
AACAGATTGC AAGAAGCCTG CAAAGACATC CTGCTGTTTA AGAACCTGGA TCCAGAACAG 
ATGTCTCAAG TATTAGATGC CATGTTTGAA AAATTGGTCA AAGAAGGGGA ACACGTAATC 
GATCAAGGTG ATGATGGTGA CAACTTTTAC GTCATCGACA GAGGAACATT TGATATTTAT 
GTAAAATGTG ATGGCGTTGG AAGATGCGTT GGTAACTATG ACAATCGTGG GAGTTTTGGA 
GAACTGGCCT TAATGTACAA TACACCCAGA GCAGCTACAA TCACTGCTAC CTCTCCTGGT 
GCTCTGTGGG GTTTGGACAG GGTGACCTTC AGGAGAATAA TAGTAAAAAA CAATGCCAAA 
AAGAGGAAGA TGTACGAGAG CTTTATTGAG TCACTGCCAT TCCTCAAGTC TCTGGAGGTT 
TCTGAACGCC TGAAGGTGGT AGATGTGATT GGCACCAAAG TTTACAACGA TGGAGAACAG 
ATCATTGCTC AGGGAGACTC GGCGGATTCG TTCTTCATTG TAGAATCTGG AGAAGTGAGA 
ATTACTATGA AGAGAAAGGG TAAATCAGAC ATCGAAGAGA ACGGTGCTGT GGAAATCGCT 
CGGTGTCTCC GGGGACAGTA TTTTGGAGAG CTTGCCCTGG TCACTAACAA GCCAAGAGCA 
GCATCTGCAC ACGCCATTGG GACTGTCAAA TGCTTAGCCA TGGATGTGCA AGCATTTGAG 
AGGCTTCTGG GACCTTGCAT GGAAATTATG AAGAGGAATA TCGCCACCTA TGAGGAGCAA 
TTAGTTGCAC TGTTTGGGAC AAACATGGAT ATTGTTGAGC CCACTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Single Turnover Autophosphorylation Cycle of the PKA RII? Holoenzyme.
PLoS biology13(7)e1002192(2015 Jul)
Zhang P,Knape MJ,Ahuja LG,Keshwani MM,King CC,Sastri M,Herberg FW,Taylor SS

The roles of the RII? linker and N-terminal cyclic nucleotide-binding domain in determining the unique structures of the type II? protein kinase A: a small angle x-ray and neutron scattering study.
The Journal of biological chemistry289(41)28505-12(2014 Oct)
Blumenthal DK,Copps J,Smith-Nguyen EV,Zhang P,Heller WT,Taylor SS

LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity.
Nature neuroscience17(3)367-76(2014 Mar)
Parisiadou L,Yu J,Sgobio C,Xie C,Liu G,Sun L,Gu XL,Lin X,Crowley NA,Lovinger DM,Cai H

Structure and allostery of the PKA RII? tetrameric holoenzyme.
Science (New York, N.Y.)335(6069)712-6(2012 Feb)
Zhang P,Smith-Nguyen EV,Keshwani MM,Deal MS,Kornev AP,Taylor SS

Protein kinase A acts at the basal body of the primary cilium to prevent Gli2 activation and ventralization of the mouse neural tube.
Development (Cambridge, England)138(22)4921-30(2011 Nov)
Tuson M,He M,Anderson KV