Alg10 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alg10
Entrez Gene ID	245960
Full Name	ALG10, alpha-1,2-glucosyltransferase
Synonyms	Alg10b,KCR1
General protein information	Preferred Names ALG10, alpha-1,2-glucosyltransferase Names putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase alpha-1,2-glucosyltransferase ALG10-A alpha-2-glucosyltransferase ALG10-B asparagine-linked glycosylation 10 homolog B (yeast, alpha-1,2-glucosyltransferase) asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog B asparagine-linked glycosylation protein 10 homolog B potassium channel regulator 1 putative alpha-1,2-glucosyltransferase ALG10-B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q35
Summary	regulatory component of non-inactivating K+ channels [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_139101.2	NP_620801.1	putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E15797g	XP_454662.1
Arabidopsis thaliana (thale cress)	AT5G02410	NP_195861.2
Pan troglodytes (chimpanzee)	ALG10	XP_003313875.1
Mus musculus (house mouse)	Alg10b	NP_001028613.1
Rattus norvegicus (Norway rat)	Alg10	NP_620801.1
Gallus gallus (chicken)	ALG10	NP_001026565.1
Danio rerio (zebrafish)	LOC100331575	XP_002661512.1
Drosophila melanogaster (fruit fly)	Alg10	NP_729680.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002420	XP_312525.4
Schizosaccharomyces pombe (fission yeast)	alg10	NP_593221.1
Xenopus tropicalis (tropical clawed frog)	alg10	NP_001016192.2
Homo sapiens (human)	ALG10	NP_116223.3
Canis lupus familiaris (dog)	ALG10	XP_534842.3
Bos taurus (cattle)	ALG10	NP_001069657.2
Saccharomyces cerevisiae (baker's yeast)	DIE2	NP_011743.2
Eremothecium gossypii	AGOS_ADL138C	NP_983958.2
Oryza sativa (rice)	Os04g0503700	NP_001053242.1

Gene Ontology
Bibliography

Related articles in PubMed

A point mutation in the gene for asparagine-linked glycosylation 10B (Alg10b) causes nonsyndromic hearing impairment in mice (Mus musculus).
Probst FJ, Corrigan RR, Del Gaudio D, Salinger AP, Lorenzo I, Gao SS, Chiu I, Xia A, Oghalai JS, Justice MJ
PloS one8(11)e80408(2013)

K+ channel regulator KCR1 suppresses heart rhythm by modulating the pacemaker current If.
Michels G, Er F, Khan IF, Endres-Becker J, Brandt MC, Gassanov N, Johns DC, Hoppe UC
PloS one3(1)e1511(2008 Jan)

The IKr drug response is modulated by KCR1 in transfected cardiac and noncardiac cell lines.
Kupershmidt S, Yang IC, Hayashi K, Wei J, Chanthaphaychith S, Petersen CI, Johns DC, George AL Jr, Roden DM, Balser JR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology17(15)2263-5(2003 Dec)

KCR1, a membrane protein that facilitates functional expression of non-inactivating K+ currents associates with rat EAG voltage-dependent K+ channels.
Hoshi N, Takahashi H, Shahidullah M, Yokoyama S, Higashida H
The Journal of biological chemistry273(36)23080-5(1998 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

observations demonstrate for the first time that KCR1 modulates I(HCN2)/I(f) channel gating and indicate that KCR1 serves as a regulator of cardiac automaticity
Title: K+ channel regulator KCR1 suppresses heart rhythm by modulating the pacemaker current If.

The following Alg10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alg10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02168	NM_139101.2 Latest version!	Rattus norvegicus ALG10, alpha-1,2-glucosyltransferase (Alg10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$279.30 $399.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alg10 ( NM_139101.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620801.1 Rattus norvegicus ALG10, alpha-1,2-glucosyltransferase (Alg10), mRNA.

Price & Availability↑

CloneID	ORa02168
Clone ID Related Accession (Same CDS sequence)	NM_139101.2
Accession Version	NM_139101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U78090.1. On Oct 26, 2007 this sequence version replaced NM_139101.1. ##Evidence-Data-START## CDS exon combination :: U78090.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCAGC TGGAGGGTTA TTACTTCTCG GCCGCCTTGA GCTGCACCTT CCTGGTGTCC 
TGCCTGCTCT TCTCCGCCTT CAGCCGCGCT CTGCGTGAGC CTTACATGGA CGAGATCTTC 
CACCTGCCGC AGGCGCAGCG CTACTGCGAG GGCCGCTTCT CACTGTCACA GTGGGATCCT 
ATGATTACTA CATTACCTGG CCTGTACCTG GTGTCGGTCG GAGTGGTCAA ACCTGCCAGC 
TGGATCCTTG GCTGGTCTGA GCATGTCGTC TGCTCCATCG GAATGCTCAG ATTTGTCAAT 
CTTCTCTTCA GTGTTGGCAA CTTCTATTTG CTGTATTTGC TCTTCAGGAA GATACAGCCC 
AGAAACAAGG CTTCTTCAAG TATCCAGAGA ATCTTGTCAA CATTAACCCT AGCAGTATTT 
CCAACCCTCT ATTTTTTTAA CTTTCTTTAC TATACAGAAG CCGGCTCTGT ATTCTTTACT 
CTTTTTGCTT ATTTGATGTG TCTTTACGGC AACCATAGGA CTTCGGCCTT GCTTGGGTTT 
TGTGGCTTCA TGTTTCGTCA GACCAATATC ATCTGGGCTG CCTTCTGTGC AGGACACATC 
ATTGCACAGA AGTGCAGTGA AGCCTGGAAG ACTGAACTAC AGAAGAAGAA GGAAGAGAGG 
CTTCCCCCGG CTAAAGGACC GCTCTCGGAA CTCAGAAGAG TTCTTCAGTT TCTGCTGATG 
TACTCCATGT CCCTTAAGAA CCTGAGTATG CTTTTCCTTT TGACCTGGCC CTACATGCTT 
CTGCTGTTGG CATTTTTCGT TTTTGTGGTG GTTAATGGTG GGATTGTCGT GGGTGATCGG 
AGTAGTCATG AGGCCTGCCT CCATTTTCCT CAGTTGTTCT ACTTCTTCTC CTTTACTGCC 
TTTTTTTCCT TTCCTCACCT ACTTTCTCCG ACCAAAGTCA AGACTTTTCT TAGCTTAGTT 
TGGAAACGTA GAGTTCAGTT CTCTGTGATT ACTTTAGTCT CGGTATTTTT GGTTTGGAAA 
TTCACTTACG TCCATAAGTA TTTACTGGCA GACAATAGGC ATTACACGTT TTATGTGTGG 
AAAAGAGTAT TTCAGAGACA TGAAATTGTC AAGTATTTAT TAGTTCCAGC CTACATGTTT 
GCTGGTTGGG CTGTAGCTGA CTCTTTAAAA TCAAAGTCAA TTTTCTGGAA TTTAATGTTT 
TTTGTATGCT TGGTTGCTTC TACGGTTCCT CAGAAACTAC TAGAATTCCG TTACTTCATT 
TTACCATACA TTATTTATAG GCTCAACATG CCTCTGCCAC CCATATCTAG ACTTGTTTGT 
GAACTGGGTT GCTATGCAGT TGTCAATTTT CTAACTTTTT ACATCTTTCT GAACAAGACT 
TTTCAGTGGT CGGATAGTCA TGACATCCAA AGGTTTATGT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620801.1
CDS	101..1525
Translation	MAQLEGYYFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQAQRYCEGRFSLSQWDPMITTLPGLYLVSVGVVKPASWILGWSEHVVCSIGMLRFVNLLFSVGNFYLLYLLFRKIQPRNKASSSIQRILSTLTLAVFPTLYFFNFLYYTEAGSVFFTLFAYLMCLYGNHRTSALLGFCGFMFRQTNIIWAAFCAGHIIAQKCSEAWKTELQKKKEERLPPAKGPLSELRRVLQFLLMYSMSLKNLSMLFLLTWPYMLLLLAFFVFVVVNGGIVVGDRSSHEACLHFPQLFYFFSFTAFFSFPHLLSPTKVKTFLSLVWKRRVQFSVITLVSVFLVWKFTYVHKYLLADNRHYTFYVWKRVFQRHEIVKYLLVPAYMFAGWAVADSLKSKSIFWNLMFFVCLVASTVPQKLLEFRYFILPYIIYRLNMPLPPISRLVCELGCYAVVNFLTFYIFLNKTFQWSDSHDIQRFMW

Target ORF information:

RefSeq Version	NM_139101.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ALG10, alpha-1,2-glucosyltransferase (Alg10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139101.2

ORF Insert Sequence:

ATGGCGCAGC TGGAGGGTTA TTACTTCTCG GCCGCCTTGA GCTGCACCTT CCTGGTGTCC 
TGCCTGCTCT TCTCCGCCTT CAGCCGCGCT CTGCGTGAGC CTTACATGGA CGAGATCTTC 
CACCTGCCGC AGGCGCAGCG CTACTGCGAG GGCCGCTTCT CACTGTCACA GTGGGATCCT 
ATGATTACTA CATTACCTGG CCTGTACCTG GTGTCGGTCG GAGTGGTCAA ACCTGCCAGC 
TGGATCCTTG GCTGGTCTGA GCATGTCGTC TGCTCCATCG GAATGCTCAG ATTTGTCAAT 
CTTCTCTTCA GTGTTGGCAA CTTCTATTTG CTGTATTTGC TCTTCAGGAA GATACAGCCC 
AGAAACAAGG CTTCTTCAAG TATCCAGAGA ATCTTGTCAA CATTAACCCT AGCAGTATTT 
CCAACCCTCT ATTTTTTTAA CTTTCTTTAC TATACAGAAG CCGGCTCTGT ATTCTTTACT 
CTTTTTGCTT ATTTGATGTG TCTTTACGGC AACCATAGGA CTTCGGCCTT GCTTGGGTTT 
TGTGGCTTCA TGTTTCGTCA GACCAATATC ATCTGGGCTG CCTTCTGTGC AGGACACATC 
ATTGCACAGA AGTGCAGTGA AGCCTGGAAG ACTGAACTAC AGAAGAAGAA GGAAGAGAGG 
CTTCCCCCGG CTAAAGGACC GCTCTCGGAA CTCAGAAGAG TTCTTCAGTT TCTGCTGATG 
TACTCCATGT CCCTTAAGAA CCTGAGTATG CTTTTCCTTT TGACCTGGCC CTACATGCTT 
CTGCTGTTGG CATTTTTCGT TTTTGTGGTG GTTAATGGTG GGATTGTCGT GGGTGATCGG 
AGTAGTCATG AGGCCTGCCT CCATTTTCCT CAGTTGTTCT ACTTCTTCTC CTTTACTGCC 
TTTTTTTCCT TTCCTCACCT ACTTTCTCCG ACCAAAGTCA AGACTTTTCT TAGCTTAGTT 
TGGAAACGTA GAGTTCAGTT CTCTGTGATT ACTTTAGTCT CGGTATTTTT GGTTTGGAAA 
TTCACTTACG TCCATAAGTA TTTACTGGCA GACAATAGGC ATTACACGTT TTATGTGTGG 
AAAAGAGTAT TTCAGAGACA TGAAATTGTC AAGTATTTAT TAGTTCCAGC CTACATGTTT 
GCTGGTTGGG CTGTAGCTGA CTCTTTAAAA TCAAAGTCAA TTTTCTGGAA TTTAATGTTT 
TTTGTATGCT TGGTTGCTTC TACGGTTCCT CAGAAACTAC TAGAATTCCG TTACTTCATT 
TTACCATACA TTATTTATAG GCTCAACATG CCTCTGCCAC CCATATCTAG ACTTGTTTGT 
GAACTGGGTT GCTATGCAGT TGTCAATTTT CTAACTTTTT ACATCTTTCT GAACAAGACT 
TTTCAGTGGT CGGATAGTCA TGACATCCAA AGGTTTATGT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A point mutation in the gene for asparagine-linked glycosylation 10B (Alg10b) causes nonsyndromic hearing impairment in mice (Mus musculus).
PloS one8(11)e80408(2013)
Probst FJ,Corrigan RR,Del Gaudio D,Salinger AP,Lorenzo I,Gao SS,Chiu I,Xia A,Oghalai JS,Justice MJ

K+ channel regulator KCR1 suppresses heart rhythm by modulating the pacemaker current If.
PloS one3(1)e1511(2008 Jan)
Michels G,Er F,Khan IF,Endres-Becker J,Brandt MC,Gassanov N,Johns DC,Hoppe UC

The IKr drug response is modulated by KCR1 in transfected cardiac and noncardiac cell lines.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology17(15)2263-5(2003 Dec)
Kupershmidt S,Yang IC,Hayashi K,Wei J,Chanthaphaychith S,Petersen CI,Johns DC,George AL Jr,Roden DM,Balser JR

KCR1, a membrane protein that facilitates functional expression of non-inactivating K+ currents associates with rat EAG voltage-dependent K+ channels.
The Journal of biological chemistry273(36)23080-5(1998 Sep)
Hoshi N,Takahashi H,Shahidullah M,Yokoyama S,Higashida H