Cyp2a3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp2a3
Entrez Gene ID	24299
Full Name	cytochrome P450, family 2, subfamily a, polypeptide 3
Synonyms	Cyp2a3a,RATCYP2A3A
General protein information	Preferred Names cytochrome P450, family 2, subfamily a, polypeptide 3 Names cytochrome P450 2A3 CYPIIA3 coumarin 7-hydroxylase cytochrome P450, family 2, subfamily A, polypeptide 3a cytochrome P450, subfamily IIA (phenobarbital-inducble)/ (cytochrome P450 IIA3)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	activates carcinogen N-nitrosomethylbenzylamine (NMBA) metabolism in the esophagus [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012542.2	NP_036674.1	cytochrome P450 2A3

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	cyp-36A1	NP_492267.1
Xenopus tropicalis (tropical clawed frog)	LOC101730237	XP_004917147.1
Homo sapiens (human)	CYP2A13	NP_000757.2
	CYP2A24	NP_001035305.1
Xenopus tropicalis (tropical clawed frog)	cyp2a6	NP_001010998.1
Xenopus tropicalis (tropical clawed frog)	LOC100496861	XP_002939423.1
Homo sapiens (human)	CYP2A6	NP_000753.3
Homo sapiens (human)	CYP2A7	NP_000755.2
Mus musculus (house mouse)	Cyp2a4	NP_034127.2
Rattus norvegicus (Norway rat)	Cyp2a3	NP_036674.1
Xenopus tropicalis (tropical clawed frog)	LOC100170416	NP_001123668.1
Mus musculus (house mouse)	Cyp2a5	NP_031838.2
Xenopus tropicalis (tropical clawed frog)	cyp2f2	NP_001010999.1
Xenopus tropicalis (tropical clawed frog)	LOC548374	XP_002939424.2
Xenopus tropicalis (tropical clawed frog)	LOC100486002	XP_004917148.1

Gene Ontology
Bibliography

Related articles in PubMed

Resveratrol improves health and survival of mice on a high-calorie diet.
Baur JA, Pearson KJ, Price NL, Jamieson HA, Lerin C, Kalra A, Prabhu VV, Allard JS, Lopez-Lluch G, Lewis K, Pistell PJ, Poosala S, Becker KG, Boss O, Gwinn D, Wang M, Ramaswamy S, Fishbein KW, Spencer RG, Lakatta EG, Le Couteur D, Shaw RJ, Navas P, Puigserver P, Ingram DK, de Cabo R, Sinclair DA
Nature444(7117)337-42(2006 Nov)

Evidence for induced microsomal bilirubin degradation by cytochrome P450 2A5.
Abu-Bakar A, Moore MR, Lang MA
Biochemical pharmacology70(10)1527-35(2005 Nov)

Transcriptional regulation of rat CYP2A3 by nuclear factor 1: identification of a novel NFI-A isoform, and evidence for tissue-selective interaction of NFI with the CYP2A3 promoter in vivo.
Ling G, Hauer CR, Gronostajski RM, Pentecost BT, Ding X
The Journal of biological chemistry279(27)27888-95(2004 Jul)

Functional role of cytochrome p-450 2a3 in N-nitrosomethylbenzylamine metabolism in rat esophagus.
Gopalakrishnan R, Gupta A, Carlton PS, Morse MA, Stoner GD
Journal of toxicology and environmental health. Part A65(15)1077-91(2002 Aug)

Identification of a novel P450 expressed in rat lung: cDNA cloning and sequence, chromosome mapping, and induction by 3-methylcholanthrene.
Kimura S, Kozak CA, Gonzalez FJ
Biochemistry28(9)3798-803(1989 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CYP2a3 trans activation requires NFIA transcription factors
Title: Transcriptional regulation of rat CYP2A3 by nuclear factor 1: identification of a novel NFI-A isoform, and evidence for tissue-selective interaction of NFI with the CYP2A3 promoter in vivo.

CYP2A3 levels in the rat esophagus can be a determinant of its ability to metabolize carcinogen N-nitrosomethylbenzylamine in vivo
Title: Functional role of cytochrome p-450 2a3 in N-nitrosomethylbenzylamine metabolism in rat esophagus.

The following Cyp2a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp2a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa39846	NM_012542.2 Latest version!	Rattus norvegicus cytochrome P450, family 2, subfamily a, polypeptide 3 (Cyp2a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp2a3 ( NM_012542.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036674.1 Rattus norvegicus cytochrome P450, family 2, subfamily a, polypeptide 3 (Cyp2a3), mRNA.

Price & Availability↑

CloneID	ORa39846
Clone ID Related Accession (Same CDS sequence)	NM_012542.2
Accession Version	NM_012542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 2A3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07002760.1, J02852.1, AABR07002761.1 and AA819453.1. On Oct 12, 2007 this sequence version replaced NM_012542.1. ##Evidence-Data-START## Transcript exon combination :: J02852.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689599, SAMEA2689604 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGCCT CAGGACTCCT TCTGGTGGCC TCAGTGGCCT TCCTCAGTGT CCTGGTCTTG 
ATGTCTGTCT GGAAGCAGAG GAAGCTCTCA GGGAAGCTGC CTCCTGGACC CACCCCATTG 
CCCTTCATCG GGAACTACCT CCAGCTGAAC ACAGAGAAAA TGTACAGCTC TCTCATGAAG 
ATCAGCCAAC GTTACGGTCC TGTATTCACC ATCCACCTGG GACCTCGCCG AGTTGTGGTG 
CTGTGCGGAC AGGAGGCAGT CAAGGAGGCT CTGGTGGACC AAGCTGAGGA ATTCAGTGGT 
CGGGGAGAGC AGGCCACCTT CGACTGGCTT TTCAAAGGCT ATGGGGTAGC CTTCAGCAGC 
GGGGAGCGAG CCAAACAGCT AAGGCGCTTC TCCATCGCCA CGCTGCGGGA CTTCGGCGTG 
GGCAAGCGTG GCATCGAGGA GCGTATCCAA GAGGAGGCGG GCTTTCTCAT CGAGTCATTT 
CGAAAGACGA ACGGTGCCCT CATTGACCCC ACCTTCTATC TGAGCCGGAC AGTCTCCAAT 
GTCATTAGCT CAATAGTCTT CGGGGACCGC TTCGACTATG AGGACAAAGA GTTCCTGTCA 
CTGCTTCGAA TGATGCTGGG AAGCTTCCAG TTCACAGCTA CCTCCACGGG GCAGCTCTAT 
GAGATGTTCT CTTCTGTGAT GAAACACCTG CCAGGCCCCC AGCAACAGGC CTTTAAGGAG 
CTGCAGGGGC TGGAGGACTT CATAACCAAG AAGGTGGAAC AGAATCAGCG CACGCTGGAT 
CCCAATTCCC CAAGGGACTT CATCGACTCT TTCCTCATCC GAATGCTGGA GGAAAAGAAG 
AACCCCAATA CTGAGTTCTA CATGAAGAAC TTGGTGCTGA CTACCCTAAA TCTCTTCTTT 
GCCGGCACAG AGACCGTCAG CACCACCCTG CGTTACGGCT TTCTGTTGCT CATGAAGCAC 
CCGGATATTG AGGCTAAGGT CCACGAGGAG ATTGACCGGG TGATTGGCAG GAACCGGCAG 
GCCAAGTATG AGGACCGAAT GAAGATGCCC TACACGGAGG CTGTGATCCA CGAGATCCAG 
AGATTTGCAG ACATGATCCC CATGGGCCTG GCTCGCAGGG TCACCAAGGA CACCAAGTTT 
CGAGAGTTCC TCCTCCCCAA GGGTACTGAA GTATTTCCTA TGCTGGGCTC TGTACTGAAA 
GACCCTAAGT TCTTCTCCAA CCCCAACGAC TTCAACCCAA AGCACTTCCT AGATGACAAG 
GGACAGTTTA AGAAGAGTGA TGCCTTTGTG CCCTTTTCCA TTGGAAAACG GTATTGTTTC 
GGGGAAGGAC TGGCAAGGAT GGAACTCTTT CTCTTCCTCA CAAACATCAT GCAGAACTTC 
TGCTTCAAAT CCCCACAGGC ACCCCAGGAC ATCGATGTGT CTCCTAGACT TGTGGGCTTT 
GCCACAATCC CACCAAACTA CACTATGAGT TTCTTGTCCC GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036674.1
CDS	1..1485
Translation	MLASGLLLVASVAFLSVLVLMSVWKQRKLSGKLPPGPTPLPFIGNYLQLNTEKMYSSLMKISQRYGPVFTIHLGPRRVVVLCGQEAVKEALVDQAEEFSGRGEQATFDWLFKGYGVAFSSGERAKQLRRFSIATLRDFGVGKRGIEERIQEEAGFLIESFRKTNGALIDPTFYLSRTVSNVISSIVFGDRFDYEDKEFLSLLRMMLGSFQFTATSTGQLYEMFSSVMKHLPGPQQQAFKELQGLEDFITKKVEQNQRTLDPNSPRDFIDSFLIRMLEEKKNPNTEFYMKNLVLTTLNLFFAGTETVSTTLRYGFLLLMKHPDIEAKVHEEIDRVIGRNRQAKYEDRMKMPYTEAVIHEIQRFADMIPMGLARRVTKDTKFREFLLPKGTEVFPMLGSVLKDPKFFSNPNDFNPKHFLDDKGQFKKSDAFVPFSIGKRYCFGEGLARMELFLFLTNIMQNFCFKSPQAPQDIDVSPRLVGFATIPPNYTMSFLSR

Target ORF information:

RefSeq Version	NM_012542.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 2, subfamily a, polypeptide 3 (Cyp2a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012542.2

ORF Insert Sequence:

ATGCTGGCCT CAGGACTCCT TCTGGTGGCC TCAGTGGCCT TCCTCAGTGT CCTGGTCTTG 
ATGTCTGTCT GGAAGCAGAG GAAGCTCTCA GGGAAGCTGC CTCCTGGACC CACCCCATTG 
CCCTTCATCG GGAACTACCT CCAGCTGAAC ACAGAGAAAA TGTACAGCTC TCTCATGAAG 
ATCAGCCAAC GTTACGGTCC TGTATTCACC ATCCACCTGG GACCTCGCCG AGTTGTGGTG 
CTGTGCGGAC AGGAGGCAGT CAAGGAGGCT CTGGTGGACC AAGCTGAGGA ATTCAGTGGT 
CGGGGAGAGC AGGCCACCTT CGACTGGCTT TTCAAAGGCT ATGGGGTAGC CTTCAGCAGC 
GGGGAGCGAG CCAAACAGCT AAGGCGCTTC TCCATCGCCA CGCTGCGGGA CTTCGGCGTG 
GGCAAGCGTG GCATCGAGGA GCGTATCCAA GAGGAGGCGG GCTTTCTCAT CGAGTCATTT 
CGAAAGACGA ACGGTGCCCT CATTGACCCC ACCTTCTATC TGAGCCGGAC AGTCTCCAAT 
GTCATTAGCT CAATAGTCTT CGGGGACCGC TTCGACTATG AGGACAAAGA GTTCCTGTCA 
CTGCTTCGAA TGATGCTGGG AAGCTTCCAG TTCACAGCTA CCTCCACGGG GCAGCTCTAT 
GAGATGTTCT CTTCTGTGAT GAAACACCTG CCAGGCCCCC AGCAACAGGC CTTTAAGGAG 
CTGCAGGGGC TGGAGGACTT CATAACCAAG AAGGTGGAAC AGAATCAGCG CACGCTGGAT 
CCCAATTCCC CAAGGGACTT CATCGACTCT TTCCTCATCC GAATGCTGGA GGAAAAGAAG 
AACCCCAATA CTGAGTTCTA CATGAAGAAC TTGGTGCTGA CTACCCTAAA TCTCTTCTTT 
GCCGGCACAG AGACCGTCAG CACCACCCTG CGTTACGGCT TTCTGTTGCT CATGAAGCAC 
CCGGATATTG AGGCTAAGGT CCACGAGGAG ATTGACCGGG TGATTGGCAG GAACCGGCAG 
GCCAAGTATG AGGACCGAAT GAAGATGCCC TACACGGAGG CTGTGATCCA CGAGATCCAG 
AGATTTGCAG ACATGATCCC CATGGGCCTG GCTCGCAGGG TCACCAAGGA CACCAAGTTT 
CGAGAGTTCC TCCTCCCCAA GGGTACTGAA GTATTTCCTA TGCTGGGCTC TGTACTGAAA 
GACCCTAAGT TCTTCTCCAA CCCCAACGAC TTCAACCCAA AGCACTTCCT AGATGACAAG 
GGACAGTTTA AGAAGAGTGA TGCCTTTGTG CCCTTTTCCA TTGGAAAACG GTATTGTTTC 
GGGGAAGGAC TGGCAAGGAT GGAACTCTTT CTCTTCCTCA CAAACATCAT GCAGAACTTC 
TGCTTCAAAT CCCCACAGGC ACCCCAGGAC ATCGATGTGT CTCCTAGACT TGTGGGCTTT 
GCCACAATCC CACCAAACTA CACTATGAGT TTCTTGTCCC GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Resveratrol improves health and survival of mice on a high-calorie diet.
Nature444(7117)337-42(2006 Nov)
Baur JA,Pearson KJ,Price NL,Jamieson HA,Lerin C,Kalra A,Prabhu VV,Allard JS,Lopez-Lluch G,Lewis K,Pistell PJ,Poosala S,Becker KG,Boss O,Gwinn D,Wang M,Ramaswamy S,Fishbein KW,Spencer RG,Lakatta EG,Le Couteur D,Shaw RJ,Navas P,Puigserver P,Ingram DK,de Cabo R,Sinclair DA

Evidence for induced microsomal bilirubin degradation by cytochrome P450 2A5.
Biochemical pharmacology70(10)1527-35(2005 Nov)
Abu-Bakar A,Moore MR,Lang MA

Transcriptional regulation of rat CYP2A3 by nuclear factor 1: identification of a novel NFI-A isoform, and evidence for tissue-selective interaction of NFI with the CYP2A3 promoter in vivo.
The Journal of biological chemistry279(27)27888-95(2004 Jul)
Ling G,Hauer CR,Gronostajski RM,Pentecost BT,Ding X

Functional role of cytochrome p-450 2a3 in N-nitrosomethylbenzylamine metabolism in rat esophagus.
Journal of toxicology and environmental health. Part A65(15)1077-91(2002 Aug)
Gopalakrishnan R,Gupta A,Carlton PS,Morse MA,Stoner GD

Identification of a novel P450 expressed in rat lung: cDNA cloning and sequence, chromosome mapping, and induction by 3-methylcholanthrene.
Biochemistry28(9)3798-803(1989 May)
Kimura S,Kozak CA,Gonzalez FJ