Cryaa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cryaa
Entrez Gene ID	24273
Full Name	crystallin, alpha A
Synonyms	Acry-1,Crya1
General protein information	Preferred Names crystallin, alpha A Names alpha-crystallin A chain Crystallin, alpha polypeptide A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	This gene encodes subunit a, one of two subunits of alpha-crystallin, which is a high molecular weight, soluble aggregate and is a member of the small heat shock protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. It acts as a molecular chaperone and is the major protein in the eye lens, maintaining the transparency and refractive index of the lens. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001289737.1	NP_001276666.1	alpha-crystallin A chain isoform 2
NM_012534.4	NP_036666.2	alpha-crystallin A chain isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CRYAA	NP_000385.1
Pan troglodytes (chimpanzee)	CRYAA	XP_514927.2
Canis lupus familiaris (dog)	CRYAA	NP_001074367.1
Mus musculus (house mouse)	Cryaa	NP_038529.1
Gallus gallus (chicken)	CRYAA	NP_001025968.1
Homo sapiens (human)	LOC102724652	XP_006723993.1
Danio rerio (zebrafish)	cryaa	NP_694482.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007159	XP_308609.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005548	XP_315550.4
Bos taurus (cattle)	CRYAA	NP_776714.1
Rattus norvegicus (Norway rat)	Cryaa	NP_036666.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007158	XP_308610.4
Xenopus tropicalis (tropical clawed frog)	LOC100488185	XP_002944595.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005547	XP_315549.4

Gene Ontology
Bibliography

Related articles in PubMed

Species-Specific Structural and Functional Divergence of ?-Crystallins: Zebrafish ?Ba- and Rodent ?A(ins)-Crystallin Encode Activated Chaperones.
Koteiche HA, Claxton DP, Mishra S, Stein RA, McDonald ET, Mchaourab HS
Biochemistry54(38)5949-58(2015 Sep)

Chaperone peptides of ?-crystallin inhibit epithelial cell apoptosis, protein insolubilization, and opacification in experimental cataracts.
Nahomi RB, Wang B, Raghavan CT, Voss O, Doseff AI, Santhoshkumar P, Nagaraj RH
The Journal of biological chemistry288(18)13022-35(2013 May)

Alpha-crystallin promotes rat olfactory ensheathing cells survival and proliferation through regulation of PI3K/Akt/mTOR signaling pathways.
Wang YH, Li YC, Huo SJ, Yin ZQ
Neuroscience letters531(2)170-5(2012 Dec)

?A-crystallin and ?B-crystallin reside in separate subcellular compartments in the developing ocular lens.
Gangalum RK, Horwitz J, Kohan SA, Bhat SP
The Journal of biological chemistry287(50)42407-16(2012 Dec)

Expression of recombinant alpha Ains-crystallin and not alpha A-crystallin inhibits bacterial growth.
Bhat SP, Nandy P, Srinivasan A, Cheng D, Sitay A
Protein engineering9(8)713-8(1996 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rat crystallin alpha A a encode activated chaperones.
Title: Species-Specific Structural and Functional Divergence of ?-Crystallins: Zebrafish ?Ba- and Rodent ?A(ins)-Crystallin Encode Activated Chaperones.

The alphaA- and alphaB-crystallin peptides inhibited stress-induced aggregation of four client proteins, and the alphaA-acetyl peptide was more effective than the native peptide against three of the client proteins.
Title: Chaperone peptides of ?-crystallin inhibit epithelial cell apoptosis, protein insolubilization, and opacification in experimental cataracts.

These findings indicated that alpha-crystallin positively regulated the activation of PI3K/Akt/mTOR signaling pathway and promote the proliferation and survival of cultured olfactory ensheathing cells
Title: Alpha-crystallin promotes rat olfactory ensheathing cells survival and proliferation through regulation of PI3K/Akt/mTOR signaling pathways.

alphaA-crystallin and alphaB-crystallin reside in separate subcellular compartments in the developing ocular lens
Title: ?A-crystallin and ?B-crystallin reside in separate subcellular compartments in the developing ocular lens.

The main differences, which could be attributed to the cataract-specific processes, are the faster decay of the content of gamma-crystallins and the significant decrease of unmodified alphaA-crystallin abundance in the senescent rat lenses.
Title: Age-related changes in the water-soluble lens protein composition of Wistar and accelerated-senescence OXYS rats.

The following Cryaa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cryaa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13655	NM_012534.4 Latest version!	Rattus norvegicus crystallin, alpha A (Cryaa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa42652	NM_001289737.1 Latest version!	Rattus norvegicus crystallin, alpha A (Cryaa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cryaa ( NM_012534.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036666.2 Rattus norvegicus crystallin, alpha A (Cryaa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa13655
Clone ID Related Accession (Same CDS sequence)	NM_012534.4
Accession Version	NM_012534.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	alpha-crystallin A chain isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC126082.1. On Jan 28, 2014 this sequence version replaced NM_012534.3. Transcript Variant: This variant (1) encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126082.1, U47922.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 7556464 ##RefSeq-Attributes-END##

ATGGACGTCA CCATCCAGCA CCCTTGGTTC AAGCGCGCCC TGGGGCCCTT CTACCCCAGC 
CGACTGTTCG ACCAGTTCTT CGGCGAGGGC CTTTTTGAAT ACGACCTGCT GCCCTTCCTG 
TCTTCCACCA TCAGCCCCTA CTACCGCCAG TCTCTCTTCC GCACAGTGTT GGACTCCGGC 
ATCTCTGAGG TCCGATCTGA CCGGGACAAG TTTGTCATCT TCTTGGATGT GAAGCACTTC 
TCTCCTGAGG ACCTCACCGT GAAGGTACTG GAAGATTTCG TGGAGATCCA TGGCAAACAC 
AACGAGAGGC AGGATGACCA TGGCTACATT TCCCGTGAAT TTCACCGTCG CTACCGTCTG 
CCTTCCAATG TGGACCAGTC CGCCCTCTCC TGCTCCTTGT CTGCGGATGG CATGCTGACC 
TTCTCTGGCC CCAAGGTCCA GTCTGGCTTG GATGCTGGCC ACAGCGAGAG GGCCATTCCC 
GTGTCACGGG AGGAGAAGCC CAGCTCGGCA CCCTCGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036666.2
CDS	60..581
Translation	MDVTIQHPWFKRALGPFYPSRLFDQFFGEGLFEYDLLPFLSSTISPYYRQSLFRTVLDSGISEVRSDRDKFVIFLDVKHFSPEDLTVKVLEDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFSGPKVQSGLDAGHSERAIPVSREEKPSSAPSS

Target ORF information:

RefSeq Version	NM_012534.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus crystallin, alpha A (Cryaa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012534.4

ORF Insert Sequence:

ATGGACGTCA CCATCCAGCA CCCTTGGTTC AAGCGCGCCC TGGGGCCCTT CTACCCCAGC 
CGACTGTTCG ACCAGTTCTT CGGCGAGGGC CTTTTTGAAT ACGACCTGCT GCCCTTCCTG 
TCTTCCACCA TCAGCCCCTA CTACCGCCAG TCTCTCTTCC GCACAGTGTT GGACTCCGGC 
ATCTCTGAGG TCCGATCTGA CCGGGACAAG TTTGTCATCT TCTTGGATGT GAAGCACTTC 
TCTCCTGAGG ACCTCACCGT GAAGGTACTG GAAGATTTCG TGGAGATCCA TGGCAAACAC 
AACGAGAGGC AGGATGACCA TGGCTACATT TCCCGTGAAT TTCACCGTCG CTACCGTCTG 
CCTTCCAATG TGGACCAGTC CGCCCTCTCC TGCTCCTTGT CTGCGGATGG CATGCTGACC 
TTCTCTGGCC CCAAGGTCCA GTCTGGCTTG GATGCTGGCC ACAGCGAGAG GGCCATTCCC 
GTGTCACGGG AGGAGAAGCC CAGCTCGGCA CCCTCGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cryaa ( NM_001289737.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001276666.1 Rattus norvegicus crystallin, alpha A (Cryaa), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa42652
Clone ID Related Accession (Same CDS sequence)	NM_001289737.1
Accession Version	NM_001289737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Rattus norvegicus(Norway rat)
Product	alpha-crystallin A chain isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U47921.1. Transcript Variant: This variant (2) includes an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2, also known as alpha Ains-crystallin) is longer, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 7556464 ##RefSeq-Attributes-END##

ATGGACGTCA CCATCCAGCA CCCTTGGTTC AAGCGCGCCC TGGGGCCCTT CTACCCCAGC 
CGACTGTTCG ACCAGTTCTT CGGCGAGGGC CTTTTTGAAT ACGACCTGCT GCCCTTCCTG 
TCTTCCACCA TCAGCCCCTA CTACCGCCAG TCTCTCTTCC GCACAGTGTT GGACTCCGGC 
ATCTCTGAGC TCATGACCCA TATGTGGTTT GTAATGCACC AACCACATGC TGGAAACCCC 
AAGAACAACC CCGGCAAGGT CCGATCTGAC CGGGACAAGT TTGTCATCTT CTTGGATGTG 
AAGCACTTCT CTCCTGAGGA CCTCACCGTG AAGGTACTGG AAGATTTCGT GGAGATCCAT 
GGCAAACACA ACGAGAGGCA GGATGACCAT GGCTACATTT CCCGTGAATT TCACCGTCGC 
TACCGTCTGC CTTCCAATGT GGACCAGTCC GCCCTCTCCT GCTCCTTGTC TGCGGATGGC 
ATGCTGACCT TCTCTGGCCC CAAGGTCCAG TCTGGCTTGG ATGCTGGCCA CAGCGAGAGG 
GCCATTCCCG TGTCACGGGA GGAGAAGCCC AGCTCGGCAC CCTCGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276666.1
CDS	15..605
Translation	MDVTIQHPWFKRALGPFYPSRLFDQFFGEGLFEYDLLPFLSSTISPYYRQSLFRTVLDSGISELMTHMWFVMHQPHAGNPKNNPGKVRSDRDKFVIFLDVKHFSPEDLTVKVLEDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFSGPKVQSGLDAGHSERAIPVSREEKPSSAPSS

Target ORF information:

RefSeq Version	NM_001289737.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus crystallin, alpha A (Cryaa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289737.1

ORF Insert Sequence:

ATGGACGTCA CCATCCAGCA CCCTTGGTTC AAGCGCGCCC TGGGGCCCTT CTACCCCAGC 
CGACTGTTCG ACCAGTTCTT CGGCGAGGGC CTTTTTGAAT ACGACCTGCT GCCCTTCCTG 
TCTTCCACCA TCAGCCCCTA CTACCGCCAG TCTCTCTTCC GCACAGTGTT GGACTCCGGC 
ATCTCTGAGC TCATGACCCA TATGTGGTTT GTAATGCACC AACCACATGC TGGAAACCCC 
AAGAACAACC CCGGCAAGGT CCGATCTGAC CGGGACAAGT TTGTCATCTT CTTGGATGTG 
AAGCACTTCT CTCCTGAGGA CCTCACCGTG AAGGTACTGG AAGATTTCGT GGAGATCCAT 
GGCAAACACA ACGAGAGGCA GGATGACCAT GGCTACATTT CCCGTGAATT TCACCGTCGC 
TACCGTCTGC CTTCCAATGT GGACCAGTCC GCCCTCTCCT GCTCCTTGTC TGCGGATGGC 
ATGCTGACCT TCTCTGGCCC CAAGGTCCAG TCTGGCTTGG ATGCTGGCCA CAGCGAGAGG 
GCCATTCCCG TGTCACGGGA GGAGAAGCCC AGCTCGGCAC CCTCGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Species-Specific Structural and Functional Divergence of ?-Crystallins: Zebrafish ?Ba- and Rodent ?A(ins)-Crystallin Encode Activated Chaperones.
Biochemistry54(38)5949-58(2015 Sep)
Koteiche HA,Claxton DP,Mishra S,Stein RA,McDonald ET,Mchaourab HS

Chaperone peptides of ?-crystallin inhibit epithelial cell apoptosis, protein insolubilization, and opacification in experimental cataracts.
The Journal of biological chemistry288(18)13022-35(2013 May)
Nahomi RB,Wang B,Raghavan CT,Voss O,Doseff AI,Santhoshkumar P,Nagaraj RH

Alpha-crystallin promotes rat olfactory ensheathing cells survival and proliferation through regulation of PI3K/Akt/mTOR signaling pathways.
Neuroscience letters531(2)170-5(2012 Dec)
Wang YH,Li YC,Huo SJ,Yin ZQ

?A-crystallin and ?B-crystallin reside in separate subcellular compartments in the developing ocular lens.
The Journal of biological chemistry287(50)42407-16(2012 Dec)
Gangalum RK,Horwitz J,Kohan SA,Bhat SP

Expression of recombinant alpha Ains-crystallin and not alpha A-crystallin inhibits bacterial growth.
Protein engineering9(8)713-8(1996 Aug)
Bhat SP,Nandy P,Srinivasan A,Cheng D,Sitay A