Cpb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpb1
Entrez Gene ID	24271
Full Name	carboxypeptidase B1
Synonyms	Cpb
General protein information	Preferred Names carboxypeptidase B1 Names carboxypeptidase B carboxypeptidase B1 (tissue) pancreatic carboxypeptidase B1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q24
Summary	putative carboxypeptidase B protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012533.2	NP_036665.2	carboxypeptidase B precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CPB1	NP_001098820.1
Rattus norvegicus (Norway rat)	Cpb1	NP_036665.1
Pan troglodytes (chimpanzee)	CPB1	XP_516808.2
	CPB1	XP_001110220.1
Danio rerio (zebrafish)	cpb1	NP_001103491.1
Xenopus tropicalis (tropical clawed frog)	LOC100494117	XP_002937383.2
Mus musculus (house mouse)	Cpb1	NP_083982.1
Gallus gallus (chicken)	CPB1	NP_001264374.1
Homo sapiens (human)	CPB1	NP_001862.2
Canis lupus familiaris (dog)	CPB1	NP_001003005.1
Drosophila melanogaster (fruit fly)	CG4017	NP_609309.1

Gene Ontology
Bibliography

Related articles in PubMed

Phosphorylation of carboxypeptidase B1 protein regulates ?-cell proliferation.
Yu SL, Han S, Kim HR, Park JW, Jin DI, Kang J
International journal of molecular medicine40(5)1397-1404(2017 Nov)

Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B.
Li S, Zhang L, Wu Q, Xin A, Zhao J, Fan L
Enzyme and microbial technology49(2)139-45(2011 Jul)

Carboxypeptidase B and other kininases of the rat coronary and mesenteric arterial bed perfusates.
Oliveira EB, Souza LL, Sivieri DO Jr, Bispo-da-Silva LB, Pereira HJ, Costa-Neto CM, Sousa MV, Salgado MC
American journal of physiology. Heart and circulatory physiology293(6)H3550-7(2007 Dec)

[Expression, purification and characterization of rat procarboxypeptidase B in Pichia pastoris].
Wang DJ, Miao L, Chen H, Li YY, Chen HP, Fang HQ
Sheng wu gong cheng xue bao = Chinese journal of biotechnology23(1)61-6(2007 Jan)

The renaturation of procarboxypeptidase B by urea gradient gel filtration and some properties of recombinant carboxypeptidase B.
Xiao-Yan Z, Su-Xia L, Qin-Sheng Y
Protein and peptide letters12(7)671-6(2005 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutants C383G, C383A had higher k(cat)/K(m) values which indicated increased catalytic abilities. And both had higher thermal stability.
Title: Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B.

Distinct bradykinin-processing pathways operate in the perfusates of rat mesenteric/coronary beds, with a substantial participation of a des-Arg(9)-BK-forming enzyme identical to pancreatic CPB.
Title: Carboxypeptidase B and other kininases of the rat coronary and mesenteric arterial bed perfusates.

Data show that specific activity of recombinant CPB is 110u/mg.
Title: [Expression, purification and characterization of rat procarboxypeptidase B in Pichia pastoris].

optimum of procarboxypeptidase B activity was achieved at pH 7-9
Title: The renaturation of procarboxypeptidase B by urea gradient gel filtration and some properties of recombinant carboxypeptidase B.

Nitric oxide production in rat lung microvascular endothelial cells is stimulated more efficiently by arginine released from carboxypeptidase substrates than free arginine.
Title: Carboxypeptidase-mediated enhancement of nitric oxide production in rat lungs and microvascular endothelial cells.

The following Cpb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa53309	NM_012533.2 Latest version!	Rattus norvegicus carboxypeptidase B1 (Cpb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpb1 ( NM_012533.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036665.2 Rattus norvegicus carboxypeptidase B1 (Cpb1), mRNA.

Price & Availability↑

CloneID	ORa53309
Clone ID Related Accession (Same CDS sequence)	NM_012533.2
Accession Version	NM_012533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-02
Organism	Rattus norvegicus(Norway rat)
Product	carboxypeptidase B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC094053.9, CF249065.1, CF249171.1 and AW916086.1. On Apr 12, 2016 this sequence version replaced NM_012533.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01906351, SAMN01906354 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGTTGCTGC TACTGGCCCT GGTGAGTGTG GCCTTGGCTC ATGCTTCCGA GGAGCACTTT 
GATGGCAACC GGGTGTACCG TGTCAGTGTA CATGGTGAAG ATCACGTCAA CTTAATTCAG 
GAGCTAGCCA ACACCAAAGA GATTGATTTC TGGAAACCAG ATTCTGCTAC ACAAGTGAAG 
CCTCTCACTA CAGTTGACTT TCATGTTAAA GCAGAAGATG TTGCTGATGT GGAGAACTTT 
CTGGAGGAGA ATGAAGTTCA CTATGAGGTA CTGATAAGCA ACGTGAGAAA TGCTCTGGAA 
TCCCAGTTTG ATAGCCACAC CCGTGCAAGT GGACACAGCT ACACCAAGTA CAACAAGTGG 
GAAACGATTG AGGCGTGGAT TCAACAAGTT GCCACTGATA ATCCAGACCT TGTCACTCAG 
AGCGTCATTG GAACCACATT TGAAGGACGT AACATGTATG TCCTCAAGAT TGGCAAAACT 
AGACCGAATA AGCCTGCCAT CTTCATCGAT TGTGGTTTCC ATGCAAGAGA GTGGATTTCT 
CCTGCATTCT GTCAGTGGTT TGTGAGAGAG GCTGTCCGTA CCTATAATCA AGAGATCCAC 
ATGAAACAGC TTCTAGATGA ACTGGATTTC TATGTTCTGC CTGTGGTCAA CATTGATGGC 
TATGTCTACA CCTGGACTAA GGACAGAATG TGGAGAAAAA CCCGCTCTAC TATGGCTGGA 
AGTTCCTGCT TGGGTGTAGA CCCCAACAGG AATTTTAATG CTGGCTGGTG TGAAGTGGGA 
GCTTCTCGGA GTCCCTGCTC TGAAACTTAC TGTGGACCAG CCCCAGAGTC TGAAAAAGAG 
ACAAAGGCCC TGGCAGATTT CATCCGCAAC AACCTCTCCA CCATCAAGGC CTACCTGACC 
ATCCACTCAT ACTCACAGAT GATGCTCTAC CCTTACTCCT ATGACTACAA ACTGCCTGAG 
AACTATGAGG AATTGAATGC CCTGGTGAAA GGTGCGGCAA AGGAGCTTGC CACTCTGCAT 
GGCACCAAGT ACACATATGG CCCAGGAGCT ACAACAATCT ATCCTGCTGC TGGGGGATCT 
GACGACTGGT CTTATGATCA GGGAATCAAA TATTCATTTA CCTTTGAACT CCGGGATACA 
GGCTTCTTTG GCTTTCTCCT TCCTGAGTCT CAGATCCGCC AGACCTGTGA GGAGACAATG 
CTTGCAGTCA AGTACATTGC CAATTATGTC CGAGAACATC TATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036665.2
CDS	24..1271
Translation	MLLLLALVSVALAHASEEHFDGNRVYRVSVHGEDHVNLIQELANTKEIDFWKPDSATQVKPLTTVDFHVKAEDVADVENFLEENEVHYEVLISNVRNALESQFDSHTRASGHSYTKYNKWETIEAWIQQVATDNPDLVTQSVIGTTFEGRNMYVLKIGKTRPNKPAIFIDCGFHAREWISPAFCQWFVREAVRTYNQEIHMKQLLDELDFYVLPVVNIDGYVYTWTKDRMWRKTRSTMAGSSCLGVDPNRNFNAGWCEVGASRSPCSETYCGPAPESEKETKALADFIRNNLSTIKAYLTIHSYSQMMLYPYSYDYKLPENYEELNALVKGAAKELATLHGTKYTYGPGATTIYPAAGGSDDWSYDQGIKYSFTFELRDTGFFGFLLPESQIRQTCEETMLAVKYIANYVREHLY

Target ORF information:

RefSeq Version	NM_012533.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carboxypeptidase B1 (Cpb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012533.2

ORF Insert Sequence:

ATGTTGCTGC TACTGGCCCT GGTGAGTGTG GCCTTGGCTC ATGCTTCCGA GGAGCACTTT 
GATGGCAACC GGGTGTACCG TGTCAGTGTA CATGGTGAAG ATCACGTCAA CTTAATTCAG 
GAGCTAGCCA ACACCAAAGA GATTGATTTC TGGAAACCAG ATTCTGCTAC ACAAGTGAAG 
CCTCTCACTA CAGTTGACTT TCATGTTAAA GCAGAAGATG TTGCTGATGT GGAGAACTTT 
CTGGAGGAGA ATGAAGTTCA CTATGAGGTA CTGATAAGCA ACGTGAGAAA TGCTCTGGAA 
TCCCAGTTTG ATAGCCACAC CCGTGCAAGT GGACACAGCT ACACCAAGTA CAACAAGTGG 
GAAACGATTG AGGCGTGGAT TCAACAAGTT GCCACTGATA ATCCAGACCT TGTCACTCAG 
AGCGTCATTG GAACCACATT TGAAGGACGT AACATGTATG TCCTCAAGAT TGGCAAAACT 
AGACCGAATA AGCCTGCCAT CTTCATCGAT TGTGGTTTCC ATGCAAGAGA GTGGATTTCT 
CCTGCATTCT GTCAGTGGTT TGTGAGAGAG GCTGTCCGTA CCTATAATCA AGAGATCCAC 
ATGAAACAGC TTCTAGATGA ACTGGATTTC TATGTTCTGC CTGTGGTCAA CATTGATGGC 
TATGTCTACA CCTGGACTAA GGACAGAATG TGGAGAAAAA CCCGCTCTAC TATGGCTGGA 
AGTTCCTGCT TGGGTGTAGA CCCCAACAGG AATTTTAATG CTGGCTGGTG TGAAGTGGGA 
GCTTCTCGGA GTCCCTGCTC TGAAACTTAC TGTGGACCAG CCCCAGAGTC TGAAAAAGAG 
ACAAAGGCCC TGGCAGATTT CATCCGCAAC AACCTCTCCA CCATCAAGGC CTACCTGACC 
ATCCACTCAT ACTCACAGAT GATGCTCTAC CCTTACTCCT ATGACTACAA ACTGCCTGAG 
AACTATGAGG AATTGAATGC CCTGGTGAAA GGTGCGGCAA AGGAGCTTGC CACTCTGCAT 
GGCACCAAGT ACACATATGG CCCAGGAGCT ACAACAATCT ATCCTGCTGC TGGGGGATCT 
GACGACTGGT CTTATGATCA GGGAATCAAA TATTCATTTA CCTTTGAACT CCGGGATACA 
GGCTTCTTTG GCTTTCTCCT TCCTGAGTCT CAGATCCGCC AGACCTGTGA GGAGACAATG 
CTTGCAGTCA AGTACATTGC CAATTATGTC CGAGAACATC TATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphorylation of carboxypeptidase B1 protein regulates ?-cell proliferation.
International journal of molecular medicine40(5)1397-1404(2017 Nov)
Yu SL,Han S,Kim HR,Park JW,Jin DI,Kang J

Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B.
Enzyme and microbial technology49(2)139-45(2011 Jul)
Li S,Zhang L,Wu Q,Xin A,Zhao J,Fan L

Carboxypeptidase B and other kininases of the rat coronary and mesenteric arterial bed perfusates.
American journal of physiology. Heart and circulatory physiology293(6)H3550-7(2007 Dec)
Oliveira EB,Souza LL,Sivieri DO Jr,Bispo-da-Silva LB,Pereira HJ,Costa-Neto CM,Sousa MV,Salgado MC

[Expression, purification and characterization of rat procarboxypeptidase B in Pichia pastoris].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology23(1)61-6(2007 Jan)
Wang DJ,Miao L,Chen H,Li YY,Chen HP,Fang HQ

The renaturation of procarboxypeptidase B by urea gradient gel filtration and some properties of recombinant carboxypeptidase B.
Protein and peptide letters12(7)671-6(2005 Oct)
Xiao-Yan Z,Su-Xia L,Qin-Sheng Y