Cad cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cad
Entrez Gene ID	24240
Full Name	carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
General protein information	Preferred Names carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase Names CAD protein carbamyl phosphatate synthetase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q14
Summary	important enzyme in the pathway of pyrimidine biosynthesis; may be regulated by the MAPK pathway [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105710.2	NP_001099180.2	CAD protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAD	XP_001155357.1
Xenopus tropicalis (tropical clawed frog)	cad	XP_004920257.1
Canis lupus familiaris (dog)	CAD	XP_540124.3
Bos taurus (cattle)	CAD	NP_001178322.1
Gallus gallus (chicken)	CAD	XP_003641116.2
Kluyveromyces lactis	KLLA0E15357g	XP_454643.1
Eremothecium gossypii	AGOS_ACR263C	NP_983665.2
Neurospora crassa	NCU08287	XP_961813.1
Danio rerio (zebrafish)	cad	NP_001009884.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000300	XP_310823.5
Caenorhabditis elegans (roundworm)	pyr-1	NP_495838.2
Saccharomyces cerevisiae (baker's yeast)	URA2	NP_012405.2
Homo sapiens (human)	CAD	NP_004332.2
Rattus norvegicus (Norway rat)	Cad	NP_001099180.2
Drosophila melanogaster (fruit fly)	r	NP_523377.1
Schizosaccharomyces pombe (fission yeast)	ura1	NP_593055.1
	CAD	XP_002799221.1
Mus musculus (house mouse)	Cad	NP_076014.1
Magnaporthe oryzae (rice blast fungus)	MGG_01537	XP_003714510.1

Gene Ontology
Bibliography

Related articles in PubMed

The dihydroorotase inhibitor 5-aminoorotic acid inhibits the metabolism in the rat of the cardioprotective drug dexrazoxane and its one-ring open metabolites.
Schroeder PE, Patel D, Hasinoff BB
Drug metabolism and disposition: the biological fate of chemicals36(9)1780-5(2008 Sep)

Mammalian pyrimidine biosynthesis: fresh insights into an ancient pathway.
Evans DR, Guy HI
The Journal of biological chemistry279(32)33035-8(2004 Aug)

ACTIVITY OF ASPARTATE TRANSCARBAMYLASE IN HEART AND LIVER OF THE DEVELOPING RAT.
NORDMANN Y, HURWITZ R, KRETCHMER N
Nature201616-7(1964 Feb)

Carbamyl phosphate-aspartate transcarbamylase activity in regenerating rat liver.
CALVA E, COHEN PP
Cancer research19(6, Part 1)679-83(1959 Jul)

Carbamyl phosphate-aspartate transcarbamylase activity in tumors.
CALVA E, LOWENSTEIN JM, COHEN PP
Cancer research19(1)101-3(1959 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that dihydroorotate dehydrogenase can generate superoxide and/or H2O2 directly at low rates and is also capable of indirect production at higher rates from other sites through its ability to reduce the ubiquinone pool.
Title: Production of superoxide/H2O2 by dihydroorotate dehydrogenase in rat skeletal muscle mitochondria.

The following Cad gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cad cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa34927	NM_001105710.2 Latest version!	Rattus norvegicus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cad ( NM_001105710.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099180.2 Rattus norvegicus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), mRNA.

Price & Availability↑

CloneID	ORa34927
Clone ID Related Accession (Same CDS sequence)	NM_001105710.2
Accession Version	NM_001105710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-08-13
Organism	Rattus norvegicus(Norway rat)
Product	CAD protein
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AABR03048817.1 and AABR03050085.1. On Oct 23, 2007 this sequence version replaced NM_001105710.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCGGCCC TGGTGTTGGA GGACGGGTCG GTGCTGCAGG GCCGGCCCTT CGGGGCCGCT 
GTGTCGACTG CTGGGGAAGT GGTGTTTCAG ACCGGCATGG TCGGCTACCC GGAGGCCCTC 
ACTGACCCTT CCTACAAAGC TCAGATCCTA GTGCTAACGT ACCCTCTCAT CGGCAACTAC 
GGTATTCCCT CAGATGAAGA GGACGAGTTC GGCCTGAGCA AGTGGTTTGA ATCCTCGGAG 
ATCCATGTGG CAGGTCTGGT GGTTGGGGAA TGCTGTCCCA CGCCCAGCCA CTGGAGTGCC 
ACCTGCACCC TGCATGAGTG GCTGCAACAG CATGGCATCC CCGGCCTGCA AGGAGTGGAC 
ACTCGGGAGC TGACTAAGAA GTTGCGGGAA CAAGGGTCTC TTTTGGGGAA GCTGGTCCAG 
AAAGGGACAG AGCCTTCAGC CCTGCCATTT GTGGACCCCA ATGCCAGGCC CCTGGCACCA 
GAGGTCTCTA TTAAGACCCC ACGGGTATTC AATGCGGGGG GTGCCCCTCG GATCTTTGCT 
GTAGACTGTG GCCTCAAGTA CAATCAGATC CGATGTCTCT GCCAGCTTGG GGCTGAGGTT 
ACCGTGGTAC CCTGGAACCA TGAATTAGAC AGTCGGAAAT ATGACGGTCT CTTCCTAAGT 
AATGGACCTG GTGATCCTGC CTCCTATCCT GGTGTGGTGT CCACACTGAA TCGAGTCTTA 
TCTGAGCCTA ATCCCCGACC TGTGTTTGGA ATCTGCCTTG GACACCAGCT ATTGGCTTTA 
GCCATTGGGG CCAAAACTTA CAAGATGAGA TACGGAAACC GAGGCCACAA CCAGCCCTGT 
TTACTGGTGG GTACTGGGCG CTGCTTTCTG ACCTCCCAGA ATCACGGGTT TGCCGTGGAC 
GCGGATTCGC TGCCAGCAGG CTGGGCTCCT CTATTTACCA ACGCCAATGA CTGTTCCAAC 
GAAGGCATTG TACACGACAA TCTGCCCTTC TTCAGCGTCC AGTTTCACCC CGAGCACCGG 
GCTGGCCCTT CAGATATGGA GCTACTCTTC GATGTGTTTC TAGAAACCGT GAGAGATACT 
GTAGCTGGGA ACGCCGAGGG CCGGACAGTA AGAGAACGTT TGGCTCAGCG CCTCTGTCCC 
CCTGAGCTTC CCATTCCTGG TTCTGGGCTC CCACCACCAC GAAAAGTTCT GATCCTGGGC 
TCGGGGGGCC TCTCCATTGG CCAGGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAAGCCCTAA AGGAGGAGAA CATCCAAACA TTACTGATCA ACCCCAACAT TGCCACAGTG 
CAGACTTCTC AGGGGCTGGC AGACAAGGTC TATTTCCTTC CCATAACACC TCACTACGTA 
ACCCAGGTGA TTCGTAATGA ACGCCCAGAT GGTATATTAC TGACTTTCGG GGGTCAGACA 
GCCCTGAACT GCGGTGTGGA GCTGACCAAA GCTGGGGTAC TAGCTCGGTA TGGGGTCCGG 
GTCTTGGGTA CACCTGTGGA GACCATTGAA CTGACTGAGG ACCGGCGAGC CTTTGCTGCC 
AGAATGGCTG AGATTGGAGA GCACGTTGCC CCCAGTGAAG CTGCAAATTC TCTTGAACAG 
GCTCAGGCAG CTGCCGAGCG GCTAGGCTAC CCTGTGCTGG TGCGAGCAGC CTTTGCCCTG 
GGTGGTCTTG GTTCTGGCTT TGCTTCCACC AAAGAGGAAC TCTCAGCTCT TGTGGCTCCA 
GCCTTTGCCC ATACCAGCCA GGTGCTGATA GACAAGTCTC TGAAGGGCTG GAAGGAGATT 
GAATATGAGG TGGTGAGAGA TGCCTATGGC AACTGCGTAA CGGTATGTAA CATGGAGAAC 
TTAGACCCAC TGGGCATCCA TACGGGGGAG TCCATAGTGG TGGCTCCAAG CCAGACACTG 
AATGACAGAG AGTACCAGCT TCTGCGGCGG ACAGCTATCA AGGTCACCCA GCACCTGGGG 
ATTGTTGGGG AGTGCAATGT GCAGTATGCC TTGAACCCCG AGTCTGAGCA GTATTACATC 
ATTGAAGTAA ATGCCAGGCT CTCCCGAAGC TCTGCCCTGG CCAGTAAGGC CACAGGCTAT 
CCACTAGCCT ATGTGGCAGC CAAGCTAGCA TTGGGCATTC CCCTGCCAGA GCTCAGAAAC 
TCTGTTACGG GGGGAACAGC AGCCTTTGAG CCCAGCCTGG ACTACTGCGT GGTAAAGATT 
CCTCGCTGGG ACCTCAGCAA GTTCCTGCGT GTCAGTACCA AGATTGGGAG CTGCATGAAG 
AGTGTTGGTG AAGTCATGGG CATCGGGCGT TCCTTTGAGG AGGCCTTCCA AAAGGCCCTG 
CGCATGGTAG ACGAGAACTG TGTAGGCTTC GACCATACAG TGAAGCCAGT CAGCGACGTG 
GAGCTGGAGA CGCCGACGGA TAAGCGGATC TTTGTGGTGG CTGCTGCTCT GTGGGCTGGC 
TACTCTGTGG AGCGCCTCTA CGAGCTCACC CGCATCGACT GCTGGTTCCT GCACCGGATG 
AAGCGCATTG TGACCCACGC CAAGTTGCTG GAACAACACC GTGGACGGCC CTTACCCCAA 
GACCTGCTGC ACCAGGCCAA GTGCCTCGGC TTCTCAGACA AGCAAATTGC TCTTGCAGTC 
CTGAGCACAG AGCTGGTTGT TCGGAAGCTG CGTCAGGAAC TGGGGATCTG CCCAGCAGTG 
AAGCAGATCG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT GTACCTGACA 
TACTGGGGCA ACACCCATGA CCTCGACTTT CGGACACCTC ACGTCCTGGT CCTTGGCTCT 
GGCGTCTACC GGATCGGCTC CAGCGTTGAG TTTGATTGGT GTGCTGTGGG CTGCATCCAG 
CAGCTCCGGA AGATGGGTTA TAAGACCATC ATGGTGAACT ACAACCCAGA GACAGTCAGC 
ACCGACTATG ATATGTGTGA CCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGTTAGAAAA CCCCGAAGGT GTGATTCTGT CCATGGGCGG GCAGCTGCCC 
AACAACATGG CTATGGCTTT GCATCGGCAG CAGTGCCGAG TGCTGGGCAC CTCCCCCGAA 
GCCATCGACT CAGCCGAGAA CCGGTTCAAG TTCTCCCGGC TCCTAGATAC CATTGGTATC 
AGCCAGCCTC AGTGGCGAGA GCTCAGTGAC CTCGAGTCCG CTCGCCAGTT CTGCCAGACC 
GTTGGGTACC CCTGCGTAGT GCGTCCCTCC TATGTGCTGA GTGGTGCTGC TATGAACGTG 
GCCTACACTG ATGGGGACCT GGAGCGCTTC CTGAGCAGTG CAGCTGCTGT CTCTAAGGAG 
CACCCTGTGG TCATCTCCAA ATTCATTCAG GAGGCAAAGG AGATTGATGT AGATGCTGTG 
GCCTGTGACG GCATCGTGTC GGCTATTGCC ATCTCTGAGC ACGTGGAGAA TGCAGGTGTG 
CATTCAGGGG ATGCCACGCT GGTGACCCCC CCACAAGACA TCACCCCCAA AACTCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTCACGGG GCCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA CGACCAGCTG AAGGTCATTG AATGTAACGT GCGGGTGTCG 
CGCTCCTTCC CGTTCGTGTC CAAGACACTT GGTGTCGACC TCGTAGCCTT GGCCACAAGG 
ATCATCATGG GGGAGAAAGT AGAGTCCGTG GGGCTCATGA CTGGCTCTGG AGTCGTGGGA 
GTAAAGGTAC CTCAGTTCTC CTTCTCACGC TTGGCCGGTG CTGACGTGGT CCTGGGTGTG 
GAGATGACCA GTACTGGAGA GGTGGCTGGC TTCGGAGAGA GCCGTTGTGA AGCCTACCTC 
AAAGCCATGC TCAGCACTGG CTTTAAGATC CCAAAGAAGA ACATCCTGCT GACCATTGGC 
AGCTACAAGA ATAAAAGTGA GCTGCTCCCA ACTGTGCGGT TGCTGGAGAG CCTGGGCTAC 
AGTCTGTATG CCAGCTTGGG AACTGCCGAC TTCTACACCG AGCACGGCGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA AGAGGCTGTG GATGGTGAGT GCCCACCACA ACGGAGCATC 
TTGGATCAGC TGGCCGAGAA TCACTTTGAG CTGGTGATTA ACCTGTCGAT GCGCGGGGCT 
GGGGGTCGGC GTCTTTCCTC CTTCGTTACC AAGGGCTACC GCACCCGGCG TCTGGCCGCG 
GATTTCTCCG TGCCTCTCAT CATTGATATC AAGTGCACCA AACTCTTCGT GGAGGCCCTA 
GGCCAGATCG GGCCAGCCCC TCCTTTGAAA GTCCACGTTG ACTGCATGAC CTCCCAGAAG 
CTAGTGCGCC TGCCTGGATT GATTGATGTC CACGTGCACC TTCGGGAACC AGGTGGGACT 
CACAAAGAGG ACTTTGCCTC AGGCACAGCT GCCGCCTTGG CTGGGGGCGT CACCATGGTT 
TGTGCTATGC CTAATACCCG GCCCCCCATC ATTGATGCCC CAGCTCTGGC GCTTGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGCTGTGAC TTTACCCTCT TCCTCGGAGC CTCATCTGAA 
AATGCAGGGA CTCTGGGTGC TGTGGCTGGG TCTGCAGCAG GCCTGAAGCT CTACCTCAAT 
GAGACCTTCT CTGAGCTACG GCTGGACAGT GTGGCCCAGT GGATGGAGCA TTTTGAAACC 
TGGCCTTCCC ACCTCCCCAT TGTGGCCCAC GCAGAGCGGC AGAGTGTCGC TGCGGTCCTC 
ATGGTGGCTC AGCTGACACG GCGTTCAGTG CACATATGTC ATGTGGCTCG GAAGGAAGAG 
ATCCTGCTGA TTAAAACTGC AAAGGCACAG GGGCTGCCAG TGACGTGTGA GGTCGCACCT 
CACCACCTGT TCCTAAACCG GGAAGACTTG GAGCGCCTGG GACCTGGGAA GGGAGAGGTC 
CGCCCTGAGC TGGGTTCCCG AGAGGATATG GAAGCCCTGT GGGAGAATAT GGCGGTCATC 
GACTGTTTTG CCTCAGACCA CGCTCCCCAT ACCTTGGAAG AGAAGTGTGG GCCTAAGCCT 
CCACCAGGCT TTCCAGGACT AGAGACCATG TTGCCGCTGC TACTGACAGC TGTGAGTGAA 
GGCCGCCTCA GTCTGGATGA CCTGCTACAG CGATTGCACC ACAACCCTCG GCGGATCTTC 
CACCTGCCTC TCCAGGAGGA CACCTATGTA GAGGTGGATC TGGAGCATGA GTGGACGGTC 
CCCAGCCACA TGCCCTTCTC TAAAGCCCAC TGGACCCCAT TTGAAGGTCA GAAGGTGAAG 
GGCACCGTGC GCCGTGTGGT CTTGAGAGGG GAGGTCGCCT ATATTGATGG ACAGGTGCTG 
GTACCCCCGG GCTATGGACA AGATGTACGG AAGTGGCCTC AGGGGGCTGT TCCTCAGCCT 
CCTCCTCCAA CTCCTACTAC CACTGAAATA ACCACGACAC CTGAGAGACC ACGCCGAGTC 
ATCCCAGGCC TTCCTGATGG CCGATTCCAC CTGCCACCTC GAATCCACCG GGCCTCTGAC 
CCAGGGCTGC CAGCTGAGGA ACCCAAAGAG AAGCCACCCA GGAAGGTAGC AGAATCAGAG 
CTGATAGGAA CCCCTGATGG CCCCTGCTAC CCTGTACCAC CAGTACCTAG ACAGGCATCA 
CCACAGAATC TAGGATCTTC TGGCCTGCTG CACCCCCAGA TGTCACCTTT ACTGCACTCC 
CTAGTGGGCC AACACATCCT GTCTGTCAAG CAGTTCACTA AGGATCAGAT GTCTCATCTG 
TTCAACGTGG CGCACACACT ACGGATGATG GTGCAGAAAG AACGGAGCCT CGACATCCTA 
AAGGGGAAGG TCATGGCCTC TATGTTCTAC GAGGTGAGCA CCCGCACCAG TAGCTCCTTC 
GCAGCAGCCA TGGCCCGGCT CGGGGGTGCC GTTCTCAGCT TCTCTGAAGC CACGTCCTCC 
GTCCAGAAGG GCGAATCCCT CGCCGACTCG GTGCAGACCA TGAGTTGCTA TGCTGATGTT 
GTTGTACTCC GTCACCCTCA GCCTGGAGCT GTGGAGCTGG CGGCCAAGCA CTGTCGAAGA 
CCAGTGATCA ATGCTGGAGA TGGAGTTGGG GAGCACCCCA CTCAGGCCCT GCTGGACATC 
TTCACCATTC GAGAAGAGCT TGGGACTGTC AATGGCATGA CGATCACTAT GGTAGGCGAC 
CTGAAGCATG GACGCACAGT GCACTCCCTG GCCTGCCTGC TCACCCAGTA CCGTGTGAGC 
CTACGCTACG TGGCACCTCC CAGCCTGCGC ATGCCATCCA GCGTGTGGGA CTTTGTGGCC 
TCCCGAGGCA CCAAGCAGGA GGAGTTCGAG AGCATCGAGG AGGCGCTGCC AGACACTGAC 
GTGCTCTACA TGACTCGGAT CCAGAAAGAA CGATTTGGCT CTTCCCAGGA ATATGAAGCT 
TGCTTTGGTC AGTTCATCCT CACTCCTCAC ATCATGACCC GGGCCAAGAA AAAGATGGTG 
GTGATGCACC CAATGCCTCG TGTCAATGAG ATAAGCGTGG AGGTGGACTC GGACCCCAGA 
GCAGCCTATT TCCGCCAAGC TGAGAACGGC ATGTACATCC GCATGGCTCT GCTTGCCACC 
GTGCTAGGCC GTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099180.2
CDS	176..6853
Translation	MAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEFGLSKWFESSEIHVAGLVVGECCPTPSHWSATCTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQKGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRIFAVDCGLKYNQIRCLCQLGAEVTVVPWNHELDSRKYDGLFLSNGPGDPASYPGVVSTLNRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDNLPFFSVQFHPEHRAGPSDMELLFDVFLETVRDTVAGNAEGRTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGILLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDVELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRMKRIVTHAKLLEQHRGRPLPQDLLHQAKCLGFSDKQIALAVLSTELVVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGNTHDLDFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVESVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFTLFLGASSENAGTLGAVAGSAAGLKLYLNETFSELRLDSVAQWMEHFETWPSHLPIVAHAERQSVAAVLMVAQLTRRSVHICHVARKEEILLIKTAKAQGLPVTCEVAPHHLFLNREDLERLGPGKGEVRPELGSREDMEALWENMAVIDCFASDHAPHTLEEKCGPKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPLQEDTYVEVDLEHEWTVPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQPPPPTPTTTEITTTPERPRRVIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKPPRKVAESELIGTPDGPCYPVPPVPRQASPQNLGSSGLLHPQMSPLLHSLVGQHILSVKQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPSSVWDFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSSQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	NM_001105710.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105710.2

ORF Insert Sequence:

ATGGCGGCCC TGGTGTTGGA GGACGGGTCG GTGCTGCAGG GCCGGCCCTT CGGGGCCGCT 
GTGTCGACTG CTGGGGAAGT GGTGTTTCAG ACCGGCATGG TCGGCTACCC GGAGGCCCTC 
ACTGACCCTT CCTACAAAGC TCAGATCCTA GTGCTAACGT ACCCTCTCAT CGGCAACTAC 
GGTATTCCCT CAGATGAAGA GGACGAGTTC GGCCTGAGCA AGTGGTTTGA ATCCTCGGAG 
ATCCATGTGG CAGGTCTGGT GGTTGGGGAA TGCTGTCCCA CGCCCAGCCA CTGGAGTGCC 
ACCTGCACCC TGCATGAGTG GCTGCAACAG CATGGCATCC CCGGCCTGCA AGGAGTGGAC 
ACTCGGGAGC TGACTAAGAA GTTGCGGGAA CAAGGGTCTC TTTTGGGGAA GCTGGTCCAG 
AAAGGGACAG AGCCTTCAGC CCTGCCATTT GTGGACCCCA ATGCCAGGCC CCTGGCACCA 
GAGGTCTCTA TTAAGACCCC ACGGGTATTC AATGCGGGGG GTGCCCCTCG GATCTTTGCT 
GTAGACTGTG GCCTCAAGTA CAATCAGATC CGATGTCTCT GCCAGCTTGG GGCTGAGGTT 
ACCGTGGTAC CCTGGAACCA TGAATTAGAC AGTCGGAAAT ATGACGGTCT CTTCCTAAGT 
AATGGACCTG GTGATCCTGC CTCCTATCCT GGTGTGGTGT CCACACTGAA TCGAGTCTTA 
TCTGAGCCTA ATCCCCGACC TGTGTTTGGA ATCTGCCTTG GACACCAGCT ATTGGCTTTA 
GCCATTGGGG CCAAAACTTA CAAGATGAGA TACGGAAACC GAGGCCACAA CCAGCCCTGT 
TTACTGGTGG GTACTGGGCG CTGCTTTCTG ACCTCCCAGA ATCACGGGTT TGCCGTGGAC 
GCGGATTCGC TGCCAGCAGG CTGGGCTCCT CTATTTACCA ACGCCAATGA CTGTTCCAAC 
GAAGGCATTG TACACGACAA TCTGCCCTTC TTCAGCGTCC AGTTTCACCC CGAGCACCGG 
GCTGGCCCTT CAGATATGGA GCTACTCTTC GATGTGTTTC TAGAAACCGT GAGAGATACT 
GTAGCTGGGA ACGCCGAGGG CCGGACAGTA AGAGAACGTT TGGCTCAGCG CCTCTGTCCC 
CCTGAGCTTC CCATTCCTGG TTCTGGGCTC CCACCACCAC GAAAAGTTCT GATCCTGGGC 
TCGGGGGGCC TCTCCATTGG CCAGGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAAGCCCTAA AGGAGGAGAA CATCCAAACA TTACTGATCA ACCCCAACAT TGCCACAGTG 
CAGACTTCTC AGGGGCTGGC AGACAAGGTC TATTTCCTTC CCATAACACC TCACTACGTA 
ACCCAGGTGA TTCGTAATGA ACGCCCAGAT GGTATATTAC TGACTTTCGG GGGTCAGACA 
GCCCTGAACT GCGGTGTGGA GCTGACCAAA GCTGGGGTAC TAGCTCGGTA TGGGGTCCGG 
GTCTTGGGTA CACCTGTGGA GACCATTGAA CTGACTGAGG ACCGGCGAGC CTTTGCTGCC 
AGAATGGCTG AGATTGGAGA GCACGTTGCC CCCAGTGAAG CTGCAAATTC TCTTGAACAG 
GCTCAGGCAG CTGCCGAGCG GCTAGGCTAC CCTGTGCTGG TGCGAGCAGC CTTTGCCCTG 
GGTGGTCTTG GTTCTGGCTT TGCTTCCACC AAAGAGGAAC TCTCAGCTCT TGTGGCTCCA 
GCCTTTGCCC ATACCAGCCA GGTGCTGATA GACAAGTCTC TGAAGGGCTG GAAGGAGATT 
GAATATGAGG TGGTGAGAGA TGCCTATGGC AACTGCGTAA CGGTATGTAA CATGGAGAAC 
TTAGACCCAC TGGGCATCCA TACGGGGGAG TCCATAGTGG TGGCTCCAAG CCAGACACTG 
AATGACAGAG AGTACCAGCT TCTGCGGCGG ACAGCTATCA AGGTCACCCA GCACCTGGGG 
ATTGTTGGGG AGTGCAATGT GCAGTATGCC TTGAACCCCG AGTCTGAGCA GTATTACATC 
ATTGAAGTAA ATGCCAGGCT CTCCCGAAGC TCTGCCCTGG CCAGTAAGGC CACAGGCTAT 
CCACTAGCCT ATGTGGCAGC CAAGCTAGCA TTGGGCATTC CCCTGCCAGA GCTCAGAAAC 
TCTGTTACGG GGGGAACAGC AGCCTTTGAG CCCAGCCTGG ACTACTGCGT GGTAAAGATT 
CCTCGCTGGG ACCTCAGCAA GTTCCTGCGT GTCAGTACCA AGATTGGGAG CTGCATGAAG 
AGTGTTGGTG AAGTCATGGG CATCGGGCGT TCCTTTGAGG AGGCCTTCCA AAAGGCCCTG 
CGCATGGTAG ACGAGAACTG TGTAGGCTTC GACCATACAG TGAAGCCAGT CAGCGACGTG 
GAGCTGGAGA CGCCGACGGA TAAGCGGATC TTTGTGGTGG CTGCTGCTCT GTGGGCTGGC 
TACTCTGTGG AGCGCCTCTA CGAGCTCACC CGCATCGACT GCTGGTTCCT GCACCGGATG 
AAGCGCATTG TGACCCACGC CAAGTTGCTG GAACAACACC GTGGACGGCC CTTACCCCAA 
GACCTGCTGC ACCAGGCCAA GTGCCTCGGC TTCTCAGACA AGCAAATTGC TCTTGCAGTC 
CTGAGCACAG AGCTGGTTGT TCGGAAGCTG CGTCAGGAAC TGGGGATCTG CCCAGCAGTG 
AAGCAGATCG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT GTACCTGACA 
TACTGGGGCA ACACCCATGA CCTCGACTTT CGGACACCTC ACGTCCTGGT CCTTGGCTCT 
GGCGTCTACC GGATCGGCTC CAGCGTTGAG TTTGATTGGT GTGCTGTGGG CTGCATCCAG 
CAGCTCCGGA AGATGGGTTA TAAGACCATC ATGGTGAACT ACAACCCAGA GACAGTCAGC 
ACCGACTATG ATATGTGTGA CCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGTTAGAAAA CCCCGAAGGT GTGATTCTGT CCATGGGCGG GCAGCTGCCC 
AACAACATGG CTATGGCTTT GCATCGGCAG CAGTGCCGAG TGCTGGGCAC CTCCCCCGAA 
GCCATCGACT CAGCCGAGAA CCGGTTCAAG TTCTCCCGGC TCCTAGATAC CATTGGTATC 
AGCCAGCCTC AGTGGCGAGA GCTCAGTGAC CTCGAGTCCG CTCGCCAGTT CTGCCAGACC 
GTTGGGTACC CCTGCGTAGT GCGTCCCTCC TATGTGCTGA GTGGTGCTGC TATGAACGTG 
GCCTACACTG ATGGGGACCT GGAGCGCTTC CTGAGCAGTG CAGCTGCTGT CTCTAAGGAG 
CACCCTGTGG TCATCTCCAA ATTCATTCAG GAGGCAAAGG AGATTGATGT AGATGCTGTG 
GCCTGTGACG GCATCGTGTC GGCTATTGCC ATCTCTGAGC ACGTGGAGAA TGCAGGTGTG 
CATTCAGGGG ATGCCACGCT GGTGACCCCC CCACAAGACA TCACCCCCAA AACTCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTCACGGG GCCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA CGACCAGCTG AAGGTCATTG AATGTAACGT GCGGGTGTCG 
CGCTCCTTCC CGTTCGTGTC CAAGACACTT GGTGTCGACC TCGTAGCCTT GGCCACAAGG 
ATCATCATGG GGGAGAAAGT AGAGTCCGTG GGGCTCATGA CTGGCTCTGG AGTCGTGGGA 
GTAAAGGTAC CTCAGTTCTC CTTCTCACGC TTGGCCGGTG CTGACGTGGT CCTGGGTGTG 
GAGATGACCA GTACTGGAGA GGTGGCTGGC TTCGGAGAGA GCCGTTGTGA AGCCTACCTC 
AAAGCCATGC TCAGCACTGG CTTTAAGATC CCAAAGAAGA ACATCCTGCT GACCATTGGC 
AGCTACAAGA ATAAAAGTGA GCTGCTCCCA ACTGTGCGGT TGCTGGAGAG CCTGGGCTAC 
AGTCTGTATG CCAGCTTGGG AACTGCCGAC TTCTACACCG AGCACGGCGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA AGAGGCTGTG GATGGTGAGT GCCCACCACA ACGGAGCATC 
TTGGATCAGC TGGCCGAGAA TCACTTTGAG CTGGTGATTA ACCTGTCGAT GCGCGGGGCT 
GGGGGTCGGC GTCTTTCCTC CTTCGTTACC AAGGGCTACC GCACCCGGCG TCTGGCCGCG 
GATTTCTCCG TGCCTCTCAT CATTGATATC AAGTGCACCA AACTCTTCGT GGAGGCCCTA 
GGCCAGATCG GGCCAGCCCC TCCTTTGAAA GTCCACGTTG ACTGCATGAC CTCCCAGAAG 
CTAGTGCGCC TGCCTGGATT GATTGATGTC CACGTGCACC TTCGGGAACC AGGTGGGACT 
CACAAAGAGG ACTTTGCCTC AGGCACAGCT GCCGCCTTGG CTGGGGGCGT CACCATGGTT 
TGTGCTATGC CTAATACCCG GCCCCCCATC ATTGATGCCC CAGCTCTGGC GCTTGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGCTGTGAC TTTACCCTCT TCCTCGGAGC CTCATCTGAA 
AATGCAGGGA CTCTGGGTGC TGTGGCTGGG TCTGCAGCAG GCCTGAAGCT CTACCTCAAT 
GAGACCTTCT CTGAGCTACG GCTGGACAGT GTGGCCCAGT GGATGGAGCA TTTTGAAACC 
TGGCCTTCCC ACCTCCCCAT TGTGGCCCAC GCAGAGCGGC AGAGTGTCGC TGCGGTCCTC 
ATGGTGGCTC AGCTGACACG GCGTTCAGTG CACATATGTC ATGTGGCTCG GAAGGAAGAG 
ATCCTGCTGA TTAAAACTGC AAAGGCACAG GGGCTGCCAG TGACGTGTGA GGTCGCACCT 
CACCACCTGT TCCTAAACCG GGAAGACTTG GAGCGCCTGG GACCTGGGAA GGGAGAGGTC 
CGCCCTGAGC TGGGTTCCCG AGAGGATATG GAAGCCCTGT GGGAGAATAT GGCGGTCATC 
GACTGTTTTG CCTCAGACCA CGCTCCCCAT ACCTTGGAAG AGAAGTGTGG GCCTAAGCCT 
CCACCAGGCT TTCCAGGACT AGAGACCATG TTGCCGCTGC TACTGACAGC TGTGAGTGAA 
GGCCGCCTCA GTCTGGATGA CCTGCTACAG CGATTGCACC ACAACCCTCG GCGGATCTTC 
CACCTGCCTC TCCAGGAGGA CACCTATGTA GAGGTGGATC TGGAGCATGA GTGGACGGTC 
CCCAGCCACA TGCCCTTCTC TAAAGCCCAC TGGACCCCAT TTGAAGGTCA GAAGGTGAAG 
GGCACCGTGC GCCGTGTGGT CTTGAGAGGG GAGGTCGCCT ATATTGATGG ACAGGTGCTG 
GTACCCCCGG GCTATGGACA AGATGTACGG AAGTGGCCTC AGGGGGCTGT TCCTCAGCCT 
CCTCCTCCAA CTCCTACTAC CACTGAAATA ACCACGACAC CTGAGAGACC ACGCCGAGTC 
ATCCCAGGCC TTCCTGATGG CCGATTCCAC CTGCCACCTC GAATCCACCG GGCCTCTGAC 
CCAGGGCTGC CAGCTGAGGA ACCCAAAGAG AAGCCACCCA GGAAGGTAGC AGAATCAGAG 
CTGATAGGAA CCCCTGATGG CCCCTGCTAC CCTGTACCAC CAGTACCTAG ACAGGCATCA 
CCACAGAATC TAGGATCTTC TGGCCTGCTG CACCCCCAGA TGTCACCTTT ACTGCACTCC 
CTAGTGGGCC AACACATCCT GTCTGTCAAG CAGTTCACTA AGGATCAGAT GTCTCATCTG 
TTCAACGTGG CGCACACACT ACGGATGATG GTGCAGAAAG AACGGAGCCT CGACATCCTA 
AAGGGGAAGG TCATGGCCTC TATGTTCTAC GAGGTGAGCA CCCGCACCAG TAGCTCCTTC 
GCAGCAGCCA TGGCCCGGCT CGGGGGTGCC GTTCTCAGCT TCTCTGAAGC CACGTCCTCC 
GTCCAGAAGG GCGAATCCCT CGCCGACTCG GTGCAGACCA TGAGTTGCTA TGCTGATGTT 
GTTGTACTCC GTCACCCTCA GCCTGGAGCT GTGGAGCTGG CGGCCAAGCA CTGTCGAAGA 
CCAGTGATCA ATGCTGGAGA TGGAGTTGGG GAGCACCCCA CTCAGGCCCT GCTGGACATC 
TTCACCATTC GAGAAGAGCT TGGGACTGTC AATGGCATGA CGATCACTAT GGTAGGCGAC 
CTGAAGCATG GACGCACAGT GCACTCCCTG GCCTGCCTGC TCACCCAGTA CCGTGTGAGC 
CTACGCTACG TGGCACCTCC CAGCCTGCGC ATGCCATCCA GCGTGTGGGA CTTTGTGGCC 
TCCCGAGGCA CCAAGCAGGA GGAGTTCGAG AGCATCGAGG AGGCGCTGCC AGACACTGAC 
GTGCTCTACA TGACTCGGAT CCAGAAAGAA CGATTTGGCT CTTCCCAGGA ATATGAAGCT 
TGCTTTGGTC AGTTCATCCT CACTCCTCAC ATCATGACCC GGGCCAAGAA AAAGATGGTG 
GTGATGCACC CAATGCCTCG TGTCAATGAG ATAAGCGTGG AGGTGGACTC GGACCCCAGA 
GCAGCCTATT TCCGCCAAGC TGAGAACGGC ATGTACATCC GCATGGCTCT GCTTGCCACC 
GTGCTAGGCC GTTTTTAG