Asip cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Asip
Entrez Gene ID	24152
Full Name	agouti signaling protein
Synonyms	ASP
General protein information	Preferred Names agouti signaling protein Names agouti-signaling protein Agouti (coat color) agouti switch protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41
Summary	This gene is involved in hair pigmentation. In several strains (ACI, IS, LEA, LEC, NER, and WKAH) this gene encodes a functional protein while in others (BN, BUF, DON, F344, SD, SHR, TM, WKY, and WTC) it lacks a 19 nt indel and is non-protein coding. [provided by RefSeq, Apr 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_052979.2	NP_443211.1	agouti-signaling protein precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ASIP	NP_996674.1
Mus musculus (house mouse)	a	NP_056585.2
Pan troglodytes (chimpanzee)	ASIP	NP_001129099.1
	LOC709156	XP_001097667.1
Homo sapiens (human)	ASIP	NP_001663.2
Canis lupus familiaris (dog)	ASIP	NP_001007264.1
Rattus norvegicus (Norway rat)	Asip	NP_443211.1

Gene Ontology
Bibliography

Related articles in PubMed

Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan.
Sasamori S, Wiewel AS, Thomson VA, Kobayashi M, Nakata K, Suzuki H
Zoological science34(6)513-522(2017 Dec)

Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation.
Hellstr?m AR, Watt B, Fard SS, Tenza D, Mannstr?m P, Narfstr?m K, Ekesten B, Ito S, Wakamatsu K, Larsson J, Ulfendahl M, Kullander K, Raposo G, Kerje S, Hallb??k F, Marks MS, Andersson L
PLoS genetics7(9)e1002285(2011 Sep)

[Methyl-containing diet of mothers affects the AGOUTI gene expression in the offspring of rats with various behavioral types].
Prasolova LA, Os'kina IN, Pliusnina IZ, Trut LN
Genetika45(5)670-6(2009 May)

Dmbx1 is essential in agouti-related protein action.
Fujimoto W, Shiuchi T, Miki T, Minokoshi Y, Takahashi Y, Takeuchi A, Kimura K, Saito M, Iwanaga T, Seino S
Proceedings of the National Academy of Sciences of the United States of America104(39)15514-9(2007 Sep)

The Effects of Genotype and Cell Environment on Melanoblast Differentiation in the House Mouse.
Markert CL, Silvers WK
Genetics41(3)429-50(1956 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Aggressive offspring is more sensitive to the mother's methyl-containing diet: the percentage of animals that are darker than control rats is higher among aggressive animals than among tame ones
Title: [Methyl-containing diet of mothers affects the AGOUTI gene expression in the offspring of rats with various behavioral types].

The following Asip gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Asip cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09173	NM_052979.2 Latest version!	Rattus norvegicus agouti signaling protein (Asip), transcript variant 1, coding, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Asip ( NM_052979.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_443211.1 Rattus norvegicus agouti signaling protein (Asip), transcript variant 1, coding, mRNA.

Price & Availability↑

CloneID	ORa09173
Clone ID Related Accession (Same CDS sequence)	NM_052979.2
Accession Version	NM_052979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Rattus norvegicus(Norway rat)
Product	agouti-signaling protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB045587.1. On Apr 22, 2015 this sequence version replaced NM_052979.1. Transcript Variant: This variant (1, coding) represents the protein-coding allele. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB045587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## polymorphic pseudogene :: PMID: 11353396 ##RefSeq-Attributes-END##

ATGGATGTCA CCCGCCTACT CCTGGCCACC CTCGTGGGCT TCCTGTGCTT CCTCACCGTC 
CACAGCCACC TGGTATTTGA GGAGACGCTT GGAGATGACA GGAGTCTAAA GAGCAACTCT 
TCCATCAACT CACTGGATTT CTCCTCTGTT TCCATTGTGG CACTGAACAA GAAATCCAAG 
AAGATCAGCA GAAAAGAAGC GGAGAAGCGG AAGAGGTCTT CCAAGAAAAA GGCTTCGATA 
AAGAAGGTGG CACGGCCCCC GCCACCTTCG CCCTGCGTGG CCACCCGCGA CAGCTGCAAG 
CCGCCTGCGC CCGCCTGCTG CAACCCGTGC GCCTCCTGCC AGTGCCGTTT CTTCGGCAGC 
GCCTGCACTT GCCGCGTACT CAACCCCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443211.1
CDS	93..488
Translation	MDVTRLLLATLVGFLCFLTVHSHLVFEETLGDDRSLKSNSSINSLDFSSVSIVALNKKSKKISRKEAEKRKRSSKKKASIKKVARPPPPSPCVATRDSCKPPAPACCNPCASCQCRFFGSACTCRVLNPNC

Target ORF information:

RefSeq Version	NM_052979.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agouti signaling protein (Asip), transcript variant 1, coding, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052979.2

ORF Insert Sequence:

ATGGATGTCA CCCGCCTACT CCTGGCCACC CTCGTGGGCT TCCTGTGCTT CCTCACCGTC 
CACAGCCACC TGGTATTTGA GGAGACGCTT GGAGATGACA GGAGTCTAAA GAGCAACTCT 
TCCATCAACT CACTGGATTT CTCCTCTGTT TCCATTGTGG CACTGAACAA GAAATCCAAG 
AAGATCAGCA GAAAAGAAGC GGAGAAGCGG AAGAGGTCTT CCAAGAAAAA GGCTTCGATA 
AAGAAGGTGG CACGGCCCCC GCCACCTTCG CCCTGCGTGG CCACCCGCGA CAGCTGCAAG 
CCGCCTGCGC CCGCCTGCTG CAACCCGTGC GCCTCCTGCC AGTGCCGTTT CTTCGGCAGC 
GCCTGCACTT GCCGCGTACT CAACCCCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan.
Zoological science34(6)513-522(2017 Dec)
Sasamori S,Wiewel AS,Thomson VA,Kobayashi M,Nakata K,Suzuki H

Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation.
PLoS genetics7(9)e1002285(2011 Sep)
Hellstr?m AR,Watt B,Fard SS,Tenza D,Mannstr?m P,Narfstr?m K,Ekesten B,Ito S,Wakamatsu K,Larsson J,Ulfendahl M,Kullander K,Raposo G,Kerje S,Hallb??k F,Marks MS,Andersson L

[Methyl-containing diet of mothers affects the AGOUTI gene expression in the offspring of rats with various behavioral types].
Genetika45(5)670-6(2009 May)
Prasolova LA,Os'kina IN,Pliusnina IZ,Trut LN

Dmbx1 is essential in agouti-related protein action.
Proceedings of the National Academy of Sciences of the United States of America104(39)15514-9(2007 Sep)
Fujimoto W,Shiuchi T,Miki T,Minokoshi Y,Takahashi Y,Takeuchi A,Kimura K,Saito M,Iwanaga T,Seino S

The Effects of Genotype and Cell Environment on Melanoblast Differentiation in the House Mouse.
Genetics41(3)429-50(1956 May)
Markert CL,Silvers WK