Caly cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Caly
Entrez Gene ID	192349
Full Name	calcyon neuron-specific vesicular protein
Synonyms	Calcyon,Drd1ip
General protein information	Preferred Names calcyon neuron-specific vesicular protein Names neuron-specific vesicular protein calcyon D1 dopamine receptor-interacting protein calcyon; D1 dopamine receptor-interacting protein dopamine receptor D1 interacting protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q41
Summary	dopamine receptor 1 (D1R) of D1-like receptors interacting protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190399.1	NP_001177328.1	neuron-specific vesicular protein calcyon
NM_138915.1	NP_620270.1	neuron-specific vesicular protein calcyon

Pathways from BioSystems

Homologies

Homo sapiens (human)	CALY	NP_056537.1
Canis lupus familiaris (dog)	CALY	XP_005637965.1
Bos taurus (cattle)	CALY	NP_001069421.1
Pan troglodytes (chimpanzee)	CALY	XP_508133.2
	CALY	XP_001093171.1
Mus musculus (house mouse)	Caly	NP_081045.2
Rattus norvegicus (Norway rat)	Caly	NP_620270.1

Gene Ontology
Bibliography

Related articles in PubMed

Dynein binds and stimulates axonal motility of the endosome adaptor and NEEP21 family member, calcyon.
Shi L, Muthusamy N, Smith D, Bergson C
The international journal of biochemistry & cell biology9093-102(2017 Sep)

Calcyon forms a novel ternary complex with dopamine D1 receptor through PSD-95 protein and plays a role in dopamine receptor internalization.
Ha CM, Park D, Han JK, Jang JI, Park JY, Hwang EM, Seok H, Chang S
The Journal of biological chemistry287(38)31813-22(2012 Sep)

Calcyon is necessary for activity-dependent AMPA receptor internalization and LTD in CA1 neurons of hippocampus.
Davidson HT, Xiao J, Dai R, Bergson C
The European journal of neuroscience29(1)42-54(2009 Jan)

Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis.
Xiao J, Dai R, Negyessy L, Bergson C
The Journal of biological chemistry281(22)15182-93(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

endocytic and cargo sorting functions of Caly in neurons
Title: Dynein binds and stimulates axonal motility of the endosome adaptor and NEEP21 family member, calcyon.

Calcyon forms a novel ternary complex with dopamine D1 receptor through PSD-95 protein and plays a role in dopamine receptor internalization
Title: Calcyon forms a novel ternary complex with dopamine D1 receptor through PSD-95 protein and plays a role in dopamine receptor internalization.

The following Caly gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Caly cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02014	NM_138915.1 Latest version!	Rattus norvegicus calcyon neuron-specific vesicular protein (Caly), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa02014	NM_001190399.1 Latest version!	Rattus norvegicus calcyon neuron-specific vesicular protein (Caly), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Caly ( NM_001190399.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001177328.1 Rattus norvegicus calcyon neuron-specific vesicular protein (Caly), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa02014
Clone ID Related Accession (Same CDS sequence)	NM_001190399.1 , NM_138915.1
Accession Version	NM_001190399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	neuron-specific vesicular protein calcyon
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060543.1 and AF303658.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: FQ213422.1, BC060543.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTGAAAC TAGGCTGCAG CTTCTCAGGG AAGCCAGGCA AGGAAACAGG AGACCAGGAT 
GGGGCTGCCA TGGACAGTGT GCCTCTGATC AGCCCCCTGG ATGTCAGCCA GCTTCAGCCA 
TCATTCCCTG ACCAGGTGGT CATCAAAACA CAGACAGAAT ATCAACTTAC CTCTGCGGAC 
CAGCCAAAGA AGTTCGCAGA TTTGGAGGGT CAGAGGCTGG CCTGCAGCCA CCCAGAGGAA 
GGACGCAGAC TGCCCACTGC AAGGATGATT GCCTTTGCTA TGGCACTTCT GGGCTGTGTG 
CTGATCATGT ACAAGGCCAT TTGGTATGAC CAGTTCACCT GCCCAGATGG CTTCCTACTT 
CGGCACAAGA TCTGCACCCC ACTGACCCTG GAGATGTACT ACACCGAGAT GGACCCCGAA 
CGCCACCGCA GCATCCTGGC AGCCATCGGG GCCTACCCAC TGAGCCGCAA GCACGGCACT 
GAGATGCCCG CCATCTGGGG AAACAGCTAC CGGGCCGGCA AGGAGGAGCA TAAGGGCACC 
ACCCCAGCTG CGATGACTGT GTCCACTGCA GCTGCAGCTG CAGCGGCAGA GGGCAACGAG 
CCGTCAGGGA AGCCTTTGGA CATGAGAGAG AAAGAAGATC CGCAGAAGGC GGAGGATGTG 
CCGTCCCAGT CCCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177328.1
CDS	103..783
Translation	MVKLGCSFSGKPGKETGDQDGAAMDSVPLISPLDVSQLQPSFPDQVVIKTQTEYQLTSADQPKKFADLEGQRLACSHPEEGRRLPTARMIAFAMALLGCVLIMYKAIWYDQFTCPDGFLLRHKICTPLTLEMYYTEMDPERHRSILAAIGAYPLSRKHGTEMPAIWGNSYRAGKEEHKGTTPAAMTVSTAAAAAAAEGNEPSGKPLDMREKEDPQKAEDVPSQSPK

Target ORF information:

RefSeq Version	NM_001190399.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcyon neuron-specific vesicular protein (Caly), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190399.1

ORF Insert Sequence:

ATGGTGAAAC TAGGCTGCAG CTTCTCAGGG AAGCCAGGCA AGGAAACAGG AGACCAGGAT 
GGGGCTGCCA TGGACAGTGT GCCTCTGATC AGCCCCCTGG ATGTCAGCCA GCTTCAGCCA 
TCATTCCCTG ACCAGGTGGT CATCAAAACA CAGACAGAAT ATCAACTTAC CTCTGCGGAC 
CAGCCAAAGA AGTTCGCAGA TTTGGAGGGT CAGAGGCTGG CCTGCAGCCA CCCAGAGGAA 
GGACGCAGAC TGCCCACTGC AAGGATGATT GCCTTTGCTA TGGCACTTCT GGGCTGTGTG 
CTGATCATGT ACAAGGCCAT TTGGTATGAC CAGTTCACCT GCCCAGATGG CTTCCTACTT 
CGGCACAAGA TCTGCACCCC ACTGACCCTG GAGATGTACT ACACCGAGAT GGACCCCGAA 
CGCCACCGCA GCATCCTGGC AGCCATCGGG GCCTACCCAC TGAGCCGCAA GCACGGCACT 
GAGATGCCCG CCATCTGGGG AAACAGCTAC CGGGCCGGCA AGGAGGAGCA TAAGGGCACC 
ACCCCAGCTG CGATGACTGT GTCCACTGCA GCTGCAGCTG CAGCGGCAGA GGGCAACGAG 
CCGTCAGGGA AGCCTTTGGA CATGAGAGAG AAAGAAGATC CGCAGAAGGC GGAGGATGTG 
CCGTCCCAGT CCCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Caly ( NM_138915.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620270.1 Rattus norvegicus calcyon neuron-specific vesicular protein (Caly), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa02014
Clone ID Related Accession (Same CDS sequence)	NM_001190399.1 , NM_138915.1
Accession Version	NM_138915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	neuron-specific vesicular protein calcyon
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF303658.1. Transcript Variant: This variant (1) represents the longer variant. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AF303658.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTGAAAC TAGGCTGCAG CTTCTCAGGG AAGCCAGGCA AGGAAACAGG AGACCAGGAT 
GGGGCTGCCA TGGACAGTGT GCCTCTGATC AGCCCCCTGG ATGTCAGCCA GCTTCAGCCA 
TCATTCCCTG ACCAGGTGGT CATCAAAACA CAGACAGAAT ATCAACTTAC CTCTGCGGAC 
CAGCCAAAGA AGTTCGCAGA TTTGGAGGGT CAGAGGCTGG CCTGCAGCCA CCCAGAGGAA 
GGACGCAGAC TGCCCACTGC AAGGATGATT GCCTTTGCTA TGGCACTTCT GGGCTGTGTG 
CTGATCATGT ACAAGGCCAT TTGGTATGAC CAGTTCACCT GCCCAGATGG CTTCCTACTT 
CGGCACAAGA TCTGCACCCC ACTGACCCTG GAGATGTACT ACACCGAGAT GGACCCCGAA 
CGCCACCGCA GCATCCTGGC AGCCATCGGG GCCTACCCAC TGAGCCGCAA GCACGGCACT 
GAGATGCCCG CCATCTGGGG AAACAGCTAC CGGGCCGGCA AGGAGGAGCA TAAGGGCACC 
ACCCCAGCTG CGATGACTGT GTCCACTGCA GCTGCAGCTG CAGCGGCAGA GGGCAACGAG 
CCGTCAGGGA AGCCTTTGGA CATGAGAGAG AAAGAAGATC CGCAGAAGGC GGAGGATGTG 
CCGTCCCAGT CCCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620270.1
CDS	162..842
Translation	MVKLGCSFSGKPGKETGDQDGAAMDSVPLISPLDVSQLQPSFPDQVVIKTQTEYQLTSADQPKKFADLEGQRLACSHPEEGRRLPTARMIAFAMALLGCVLIMYKAIWYDQFTCPDGFLLRHKICTPLTLEMYYTEMDPERHRSILAAIGAYPLSRKHGTEMPAIWGNSYRAGKEEHKGTTPAAMTVSTAAAAAAAEGNEPSGKPLDMREKEDPQKAEDVPSQSPK

Target ORF information:

RefSeq Version	NM_138915.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcyon neuron-specific vesicular protein (Caly), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138915.1

ORF Insert Sequence:

ATGGTGAAAC TAGGCTGCAG CTTCTCAGGG AAGCCAGGCA AGGAAACAGG AGACCAGGAT 
GGGGCTGCCA TGGACAGTGT GCCTCTGATC AGCCCCCTGG ATGTCAGCCA GCTTCAGCCA 
TCATTCCCTG ACCAGGTGGT CATCAAAACA CAGACAGAAT ATCAACTTAC CTCTGCGGAC 
CAGCCAAAGA AGTTCGCAGA TTTGGAGGGT CAGAGGCTGG CCTGCAGCCA CCCAGAGGAA 
GGACGCAGAC TGCCCACTGC AAGGATGATT GCCTTTGCTA TGGCACTTCT GGGCTGTGTG 
CTGATCATGT ACAAGGCCAT TTGGTATGAC CAGTTCACCT GCCCAGATGG CTTCCTACTT 
CGGCACAAGA TCTGCACCCC ACTGACCCTG GAGATGTACT ACACCGAGAT GGACCCCGAA 
CGCCACCGCA GCATCCTGGC AGCCATCGGG GCCTACCCAC TGAGCCGCAA GCACGGCACT 
GAGATGCCCG CCATCTGGGG AAACAGCTAC CGGGCCGGCA AGGAGGAGCA TAAGGGCACC 
ACCCCAGCTG CGATGACTGT GTCCACTGCA GCTGCAGCTG CAGCGGCAGA GGGCAACGAG 
CCGTCAGGGA AGCCTTTGGA CATGAGAGAG AAAGAAGATC CGCAGAAGGC GGAGGATGTG 
CCGTCCCAGT CCCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynein binds and stimulates axonal motility of the endosome adaptor and NEEP21 family member, calcyon.
The international journal of biochemistry & cell biology9093-102(2017 Sep)
Shi L,Muthusamy N,Smith D,Bergson C

Calcyon forms a novel ternary complex with dopamine D1 receptor through PSD-95 protein and plays a role in dopamine receptor internalization.
The Journal of biological chemistry287(38)31813-22(2012 Sep)
Ha CM,Park D,Han JK,Jang JI,Park JY,Hwang EM,Seok H,Chang S

Calcyon is necessary for activity-dependent AMPA receptor internalization and LTD in CA1 neurons of hippocampus.
The European journal of neuroscience29(1)42-54(2009 Jan)
Davidson HT,Xiao J,Dai R,Bergson C

Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis.
The Journal of biological chemistry281(22)15182-93(2006 Jun)
Xiao J,Dai R,Negyessy L,Bergson C