Pigl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pigl
Entrez Gene ID	192263
Full Name	phosphatidylinositol glycan anchor biosynthesis, class L
General protein information	Preferred Names phosphatidylinositol glycan anchor biosynthesis, class L Names N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase PIG-L phosphatidylinositol glycan, class L phosphatidylinositol-glycan biosynthesis class L protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q23
Summary	catalyzes the N-deacetylation of N-acetyl-D-glucosaminylphosphatidylinositol in the second step of GPI anchor biosynthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008767767.1	XP_008765989.1	N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase isoform X1
NM_138901.2	NP_620256.1	N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pigl	NP_001243584.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001955	XP_003435975.1
Magnaporthe oryzae (rice blast fungus)	MGG_05020	XP_003712526.1
Oryza sativa (rice)	Os04g0678800	NP_001054275.1
Homo sapiens (human)	PIGL	NP_004269.1
Pan troglodytes (chimpanzee)	PIGL	XP_523760.3
Bos taurus (cattle)	PIGL	NP_001093781.1
Drosophila melanogaster (fruit fly)	CG4433	NP_001247201.1
Schizosaccharomyces pombe (fission yeast)	gpi12	NP_593887.1
	PIGL	XP_001088273.2
Mus musculus (house mouse)	Pigl	NP_001034625.1
Rattus norvegicus (Norway rat)	Pigl	NP_620256.1
Gallus gallus (chicken)	PIGL	XP_415845.4
Kluyveromyces lactis	KLLA0D01430g	XP_453132.1
Canis lupus familiaris (dog)	PIGL	XP_536652.2
Caenorhabditis elegans (roundworm)	Y52B11C.1	NP_492873.2
Arabidopsis thaliana (thale cress)	AT2G27340	NP_973543.1
Saccharomyces cerevisiae (baker's yeast)	GPI12	NP_014008.1
Eremothecium gossypii	AGOS_ABL120W	NP_982827.1
Neurospora crassa	NCU09510	XP_958505.2
Arabidopsis thaliana (thale cress)	AT3G58130	NP_001030882.1
Xenopus tropicalis (tropical clawed frog)	pigl	XP_002938967.1

Gene Ontology
Bibliography

Related articles in PubMed

The N-acetyl-D-glucosaminylphosphatidylinositol De-N-acetylase of glycosylphosphatidylinositol biosynthesis is a zinc metalloenzyme.
Urbaniak MD, Crossman A, Chang T, Smith TK, van Aalten DM, Ferguson MA
The Journal of biological chemistry280(24)22831-8(2005 Jun)

Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis.
Watanabe R, Ohishi K, Maeda Y, Nakamura N, Kinoshita T
The Biochemical journal339 ( Pt 1)185-92(1999 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

N-acetyl-D-glucosaminylphosphatidylinositol De-N-acetylase of glycosylphosphatidylinositol biosynthesis is a zinc metalloenzyme
Title: The N-acetyl-D-glucosaminylphosphatidylinositol De-N-acetylase of glycosylphosphatidylinositol biosynthesis is a zinc metalloenzyme.

The following Pigl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pigl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01951	NM_138901.2 Latest version!	Rattus norvegicus phosphatidylinositol glycan anchor biosynthesis, class L (Pigl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa44378	XM_008767767.1 Latest version!	Rattus norvegicus phosphatidylinositol glycan anchor biosynthesis, class L (Pigl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pigl ( NM_138901.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620256.1 Rattus norvegicus phosphatidylinositol glycan anchor biosynthesis, class L (Pigl), mRNA.

Price & Availability↑

CloneID	ORa01951
Clone ID Related Accession (Same CDS sequence)	NM_138901.2
Accession Version	NM_138901.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC074020.1. On Jun 10, 2007 this sequence version replaced NM_138901.1. ##Evidence-Data-START## Transcript exon combination :: BC074020.1, CK356582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGTGG TGGGTCTCTT GTGTGTGGCC GTGGCTGTCC TGACTTGGGG CTTCCTCCGG 
GTTTGGAACT CAGCGGAACG AATGAGGTCT CCGGAGCAGG CGGGCCTACC GGGAGCCGGA 
AGCCGGGCCC TTGTAGTGAT CGCGCACCCG GACGATGAAG CCATGTTCTT TGCTCCCACG 
ATACTAGGCT TGGCACGCCT GAAGCAGCAG GTGTCCCTGC TCTGCTTCTC CTCAGGAAAT 
TACTACAATC AAGGTGAGAT TCGAAAGAAA GAACTTCTGC AGAGCTGTGC TGTCTTGGGG 
ATTCCACCTT CCCGTGTAAT GATTATTGAC AAGAGGGAGT TTCCAGATGA TCCAGAAGTG 
CAGTGGGACA CAGAGCATGT GGCCAGCACC ATCCTCCAGC ACATACATGC CAATGCCACC 
GACTTGGTGG TGACTTTTGA TGCAGAAGGA GTCAGTGGTC ACAGCAACCA TATTGCCTTG 
TACAAAGCTG TGAGGGCCCT GCACTCAGGA GGGAAGCTGC CTGAAGGGTG CTCTGTACTT 
ACTCTCCAGT CTGTGAACGT GCTTCGGAAG TATGTCTTCC TCCTGGATCT CCCCTGGACT 
CTACTGTCGC CCCAGGGTGT TCTCTTTGTG CTCACCAGCA AAGAAGTGGC ACAGGCCAAG 
AAAGCCATGT CCTGCCACCG CAGTCAGCTG CTCTGGTTCC GCCACCTCTA CACCGTCTTC 
TCGAGATACA TGAGCGTCAA CTCGCTGCAG TTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620256.1
CDS	10..768
Translation	MEVVGLLCVAVAVLTWGFLRVWNSAERMRSPEQAGLPGAGSRALVVIAHPDDEAMFFAPTILGLARLKQQVSLLCFSSGNYYNQGEIRKKELLQSCAVLGIPPSRVMIIDKREFPDDPEVQWDTEHVASTILQHIHANATDLVVTFDAEGVSGHSNHIALYKAVRALHSGGKLPEGCSVLTLQSVNVLRKYVFLLDLPWTLLSPQGVLFVLTSKEVAQAKKAMSCHRSQLLWFRHLYTVFSRYMSVNSLQLL

Target ORF information:

RefSeq Version	NM_138901.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphatidylinositol glycan anchor biosynthesis, class L (Pigl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138901.2

ORF Insert Sequence:

ATGGAAGTGG TGGGTCTCTT GTGTGTGGCC GTGGCTGTCC TGACTTGGGG CTTCCTCCGG 
GTTTGGAACT CAGCGGAACG AATGAGGTCT CCGGAGCAGG CGGGCCTACC GGGAGCCGGA 
AGCCGGGCCC TTGTAGTGAT CGCGCACCCG GACGATGAAG CCATGTTCTT TGCTCCCACG 
ATACTAGGCT TGGCACGCCT GAAGCAGCAG GTGTCCCTGC TCTGCTTCTC CTCAGGAAAT 
TACTACAATC AAGGTGAGAT TCGAAAGAAA GAACTTCTGC AGAGCTGTGC TGTCTTGGGG 
ATTCCACCTT CCCGTGTAAT GATTATTGAC AAGAGGGAGT TTCCAGATGA TCCAGAAGTG 
CAGTGGGACA CAGAGCATGT GGCCAGCACC ATCCTCCAGC ACATACATGC CAATGCCACC 
GACTTGGTGG TGACTTTTGA TGCAGAAGGA GTCAGTGGTC ACAGCAACCA TATTGCCTTG 
TACAAAGCTG TGAGGGCCCT GCACTCAGGA GGGAAGCTGC CTGAAGGGTG CTCTGTACTT 
ACTCTCCAGT CTGTGAACGT GCTTCGGAAG TATGTCTTCC TCCTGGATCT CCCCTGGACT 
CTACTGTCGC CCCAGGGTGT TCTCTTTGTG CTCACCAGCA AAGAAGTGGC ACAGGCCAAG 
AAAGCCATGT CCTGCCACCG CAGTCAGCTG CTCTGGTTCC GCCACCTCTA CACCGTCTTC 
TCGAGATACA TGAGCGTCAA CTCGCTGCAG TTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pigl ( XM_008767767.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765989.1 Rattus norvegicus phosphatidylinositol glycan anchor biosynthesis, class L (Pigl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44378
Clone ID Related Accession (Same CDS sequence)	XM_008767767.1
Accession Version	XM_008767767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTGG TGGGTCTCTT GTGTGTGGCC GTGGCTGTCC TGACTTGGGG CTTCCTCCGG 
GTTTGGAACT CAGCGGAACG AATGAGGTCT CCGGAGCAGG CGGGCCTACC GGGAGCCGGA 
AGCCGGGCCC TTGTAGTGAT CGCGCACCCG GACGATGAAG CCATGTTCTT TGCTCCCACG 
ATACTAGGCT TGGCACGCCT GAAGCAGCAG GTGTCCCTGC TCTGCTTCTC CTCAGGAAAT 
TACTACAATC AAGGTGAGAT TCGAAAGAAA GAACTTCTGC AGAGCTGTGC TGTCTTGGGG 
ATTCCACCTT CCCGTGTAAT GATTATTGAC AAGAGGGAGT TTCCAGATGA TCCAGAAGTG 
CAGTGGGACA CAGAGCATGT GGCCAGCACC ATCCTCCAGC ACATACATGC CAATGCCACC 
GACTTGGTGG TGACTTTTGA TGCAGAAGGA GTCAGTGGTC ACAGCAACCA TATTGCCTTG 
TACAAAGCTG TGAGGGCCCT GCACTCAGGA GGGAAGCTGC CTGAAGGGTG CTCTGTACTT 
ACTCTCCAGT CTGTGAACGT GCTTCGGAAG TATGTCTTCC TCCTGGATCT CCCCTGGACT 
CTACTGTCGC CCCAGGGTGT TCTCTTTGTG CTCACCAGCA AAGAAGTGGC ACAGGCCAAG 
GTAAGACATG CATTTCCTGG ACACTGCACT GGCACTGTGA CCCCAGCCTC AGGTCCCTTG 
GCTCCTCAAA GCCCTGCTAC CTGCAGAGCA CTGACCACAG GCACCGACAC TGCCCCACAT 
GCTAGGTTAT TGCAGCTGTA CCCGACTGAA CACATTTTCA CTGATTTGGG CTTTTTTCCT 
GATGATAAAA TTTATAATCA TGCTCATTTT AAGAAACTTA GAAATACATA CCATCATCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765989.1
CDS	6..908
Translation	MEVVGLLCVAVAVLTWGFLRVWNSAERMRSPEQAGLPGAGSRALVVIAHPDDEAMFFAPTILGLARLKQQVSLLCFSSGNYYNQGEIRKKELLQSCAVLGIPPSRVMIIDKREFPDDPEVQWDTEHVASTILQHIHANATDLVVTFDAEGVSGHSNHIALYKAVRALHSGGKLPEGCSVLTLQSVNVLRKYVFLLDLPWTLLSPQGVLFVLTSKEVAQAKVRHAFPGHCTGTVTPASGPLAPQSPATCRALTTGTDTAPHARLLQLYPTEHIFTDLGFFPDDKIYNHAHFKKLRNTYHHQ

Target ORF information:

RefSeq Version	XM_008767767.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphatidylinositol glycan anchor biosynthesis, class L (Pigl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767767.1

ORF Insert Sequence:

ATGGAAGTGG TGGGTCTCTT GTGTGTGGCC GTGGCTGTCC TGACTTGGGG CTTCCTCCGG 
GTTTGGAACT CAGCGGAACG AATGAGGTCT CCGGAGCAGG CGGGCCTACC GGGAGCCGGA 
AGCCGGGCCC TTGTAGTGAT CGCGCACCCG GACGATGAAG CCATGTTCTT TGCTCCCACG 
ATACTAGGCT TGGCACGCCT GAAGCAGCAG GTGTCCCTGC TCTGCTTCTC CTCAGGAAAT 
TACTACAATC AAGGTGAGAT TCGAAAGAAA GAACTTCTGC AGAGCTGTGC TGTCTTGGGG 
ATTCCACCTT CCCGTGTAAT GATTATTGAC AAGAGGGAGT TTCCAGATGA TCCAGAAGTG 
CAGTGGGACA CAGAGCATGT GGCCAGCACC ATCCTCCAGC ACATACATGC CAATGCCACC 
GACTTGGTGG TGACTTTTGA TGCAGAAGGA GTCAGTGGTC ACAGCAACCA TATTGCCTTG 
TACAAAGCTG TGAGGGCCCT GCACTCAGGA GGGAAGCTGC CTGAAGGGTG CTCTGTACTT 
ACTCTCCAGT CTGTGAACGT GCTTCGGAAG TATGTCTTCC TCCTGGATCT CCCCTGGACT 
CTACTGTCGC CCCAGGGTGT TCTCTTTGTG CTCACCAGCA AAGAAGTGGC ACAGGCCAAG 
GTAAGACATG CATTTCCTGG ACACTGCACT GGCACTGTGA CCCCAGCCTC AGGTCCCTTG 
GCTCCTCAAA GCCCTGCTAC CTGCAGAGCA CTGACCACAG GCACCGACAC TGCCCCACAT 
GCTAGGTTAT TGCAGCTGTA CCCGACTGAA CACATTTTCA CTGATTTGGG CTTTTTTCCT 
GATGATAAAA TTTATAATCA TGCTCATTTT AAGAAACTTA GAAATACATA CCATCATCAG 
TAA