Polk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polk
Entrez Gene ID	171525
Full Name	DNA polymerase kappa
Synonyms	Dinb1
General protein information	Preferred Names DNA polymerase kappa Names DNA polymerase kappa DNA-directed DNA polymerase kappa polymerase (DNA directed) kappa polymerase (DNA) kappa
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	may mediate the increase of A:T to C:G transversions [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138516.1	NP_612525.1	DNA polymerase kappa

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Polk	NP_612525.1
Xenopus tropicalis (tropical clawed frog)	polk	NP_001005705.1
Mus musculus (house mouse)	Polk	NP_036178.1
Caenorhabditis elegans (roundworm)	polk-1	NP_001122692.1
Schizosaccharomyces pombe (fission yeast)	kpa1	NP_587767.2
Homo sapiens (human)	POLK	NP_057302.1
Canis lupus familiaris (dog)	POLK	XP_536321.2
Gallus gallus (chicken)	POLK	NP_989514.1
	POLK	XP_001105220.1
Bos taurus (cattle)	POLK	NP_001095728.1
Magnaporthe oryzae (rice blast fungus)	MGG_07238	XP_003715457.1
Pan troglodytes (chimpanzee)	POLK	XP_001149179.1
Danio rerio (zebrafish)	polk	XP_691219.4
Neurospora crassa	NCU02457	XP_959512.1

Gene Ontology
Bibliography

Related articles in PubMed

RNA m
Xiang Y, Laurent B, Hsu CH, Nachtergaele S, Lu Z, Sheng W, Xu C, Chen H, Ouyang J, Wang S, Ling D, Hsu PH, Zou L, Jambhekar A, He C, Shi Y
Nature543(7646)573-576(2017 03)

DNA polymerases and repair synthesis in NER in human cells.
Lehmann AR
DNA repair10(7)730-3(2011 Jul)

Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells.
Ogi T, Limsirichaikul S, Overmeer RM, Volker M, Takenaka K, Cloney R, Nakazawa Y, Niimi A, Miki Y, Jaspers NG, Mullenders LH, Yamashita S, Fousteri MI, Lehmann AR
Molecular cell37(5)714-27(2010 Mar)

DNA polymerase kappa deficiency does not affect somatic hypermutation in mice.
Schenten D, Gerlach VL, Guo C, Velasco-Miguel S, Hladik CL, White CL, Friedberg EC, Rajewsky K, Esposito G
European journal of immunology32(11)3152-60(2002 Nov)

The absence of Mth1 inactivation and DNA polymerase kappa overexpression in rat mammary carcinomas with frequent A:T to C:G transversions.
Okochi E, Ichimura S, Sugimura T, Ushijima T
Japanese journal of cancer research : Gann93(5)501-6(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01527	NM_138516.1 Latest version!	Rattus norvegicus DNA polymerase kappa (Polk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$503.30 $719.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polk ( NM_138516.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_612525.1 Rattus norvegicus DNA polymerase kappa (Polk), mRNA.

Price & Availability↑

CloneID	ORa01527
Clone ID Related Accession (Same CDS sequence)	NM_138516.1
Accession Version	NM_138516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	DNA polymerase kappa
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166778.1. On or before Jun 8, 2008 this sequence version replaced XM_342178.3, XM_001060864.1. ##Evidence-Data-START## Transcript exon combination :: BC166778.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01906351 [ECO:0000350] ##Evidence-Data-END##

ATGGATAACA CAAAGGAAAA GAACAACTTC AAAGACGCCC TTCTGCTCCG CATGGGACTA 
AATGATAACA AGGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAACTCA AGAAGGAGAA GCAAGTCAAT 
CAACGGATTG AAAACATGAT GCAGCAAAAG GCGCAAATTA CCAGCCAGCA ACTAAGGAAG 
GCCCAGCTAC AGGTTGACAG ATTTGCGATG GGATTAGAAC GGAACCGGAA TTTGAACAAC 
ACCATAGTTC ACGTGGACAT GGATGCTTTC TATGCGGCCG TAGAAATGAG GGACAACCCG 
GAACTGAAGG AGAAGCCCAT CGCTGTAGGA TCCATGAGCA TGCTGGCTAC TTCAAATTAC 
CACGCAAGGA GGTTTGGGGT TCGCGCAGCC ATGCCAGGAT TTATTGCCAA GAGACTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAGTATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TCGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAGGCCTAC 
TTGAATATAA CACAGCACTT ACAGGAAAGG CAAGGTTGGC CTGAGGACAA AAGAAGATAT 
TTCATCAAAA TGGGAAACTG TAGAAAAATT GACACACCCC GGCAGGAAGC TGATGAGCTG 
ACTGAGCGGG AGCGCTCCAT CTCCCCACTG CTTTTTGAAG ATAGCCCTCC TGATTTGCAA 
CCCCAAGGAA GTCCTTTCCA ACTGAACTCT GAAGAACAAA ACAATCCTCA AATAGCCCAA 
AATCCAGTTG TTTTTGGAAC ATCAGCTGAG GAAGTGGTAA AGGAAATTCG CTTCAGAATT 
GAACAGAAAA CAACGCTAAC AGCCAGCGCA GGCATCGCCC CTAATACAAT GTTAGCAAAA 
GTGTGCAGTG ATAAGAATAA ACCAAATGGA CAGTACCAGA TCGTCCCCAG CAGGAGCGCA 
GTGATGGACT TCATCAAGGA CTTGCCTATT CGAAAGGTTT CTGGAATAGG AAAAGTTACG 
GAGAAAATGT TGCTGGCTCT CGGGATTGTT ACTTGCACAG AACTCTACCA ACAGAGAGCG 
TTGCTTTCTC TCCTTTTCTC GGAGTCATCC TGGCACTATT TTCTTCATGT TGCACTGGGC 
CTGGGTTCAA CAGACCTGGC GAGGGATGGA GAAAGGAAAA GTATGAGCGT TGAAAGGACA 
TTTAGTGAGA TCAATAAAAC AGAGGAACAG TACAGCTTGT GCCAAGAACT GTGCACTGAG 
CTCGCCCACG ATCTCCAGAA GGAAGGACTT AAGGGCAGAA CTGTCACCAT CAAGCTGAAG 
AATGTGGATT TTGAAGTAAA AACCCGCGCA TCTACAGTAC CGTCCGCCAT TTCTACTGCA 
GAGGACATAT TTGCCGTTGC CAAGGAGCTG CTAAGGACAG AAGTCACTGC AGGCTCTCCA 
CAGCCCCTGC GGCTAAGACT GATGGGTGTT CGGATGTCTA CTTTCTCCAA CGACGATGAC 
AGGAAGCACC AACAAAGGAG CATCATTGGC TTCTTACAAG CTGGAAACCA AGGTTTGTCA 
CCCACTGGGT GTACTGCAGA CAGAACTGAC AAAACTGAGC TCGTAAAGCC CTTAGAAACG 
TCTCGTAAGA AGAGTTTCTT TGATAAAAAG CGATCAGAAA GGATCCCGAA CTGTCAAGAC 
CCACCCAGAT GTAAAATCGC GGGTGAGCAA GCTTTACAGG CCTCGGAACC ATCCCAAGCG 
TTGAAGAAGA TGAGCGAGAG TTTCAAAACC TCAGAGAATT CAAATGACCC TCAGACATTT 
ATATGTCCAG TTTGCTTTAG GGAGCAAGAA GGCATCAGTC TGGAAGCCTT TAATGAACAT 
GTAGATGCAT GTCTCGATGG ACCGTCCACC AGTGAGAACT CAGAGATGCC CAGTTACTCA 
CATGCTTCGT CTGCGGACAT TGGTCAGCAG GCACACCGTG CTGTTCCACT GTGGGAGAAA 
CGGGGGCATG GAGATGGAGA GATCACCTCA GGAGATGGTG CAGATCTAAC AGAGACTGAA 
GACAGATCGC CGAAAGCAGC AAGCATGGAC GCTGTAGGGA ATAATCACAG CGAAGGGGAG 
GGATATCCTG ATAGTCAGGG CAAGTCCTGC CCTCTGTCAC CCGCAAACGA AACTGTCAGC 
ACGTTAAGTA GGGAAGACTC TGCCCAGCCC TGTACAGATG AGGTAGTGAC AGGACGAGCT 
CTAGTTTGTC CCGTTTGTAA CCTAGAACAA GAGACGTCTG ATCTCACCCT CTTCAACATC 
CACGTGGACA TTTGCTTAAA TAAAGGCATC ATCCAAGAAC TGAGAAACAG CGAAGTTAAT 
TCAGTTAAGC AACCCAAAGG GAGCACGAGA AGCACTGACA GACTTCAGAA GGCTTCAGGA 
AAGACAAAAA GGGCAGGCCG GAAGACAAAG GGCTCAACAT CGAAGAGAAC AAAACCCCGA 
GATCCCAGAC ACACCCTTGA TGGATTTTTT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612525.1
CDS	201..2756
Translation	MDNTKEKNNFKDALLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMGLERNRNLNNTIVHVDMDAFYAAVEMRDNPELKEKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQERQGWPEDKRRYFIKMGNCRKIDTPRQEADELTERERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNPQIAQNPVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQIVPSRSAVMDFIKDLPIRKVSGIGKVTEKMLLALGIVTCTELYQQRALLSLLFSESSWHYFLHVALGLGSTDLARDGERKSMSVERTFSEINKTEEQYSLCQELCTELAHDLQKEGLKGRTVTIKLKNVDFEVKTRASTVPSAISTAEDIFAVAKELLRTEVTAGSPQPLRLRLMGVRMSTFSNDDDRKHQQRSIIGFLQAGNQGLSPTGCTADRTDKTELVKPLETSRKKSFFDKKRSERIPNCQDPPRCKIAGEQALQASEPSQALKKMSESFKTSENSNDPQTFICPVCFREQEGISLEAFNEHVDACLDGPSTSENSEMPSYSHASSADIGQQAHRAVPLWEKRGHGDGEITSGDGADLTETEDRSPKAASMDAVGNNHSEGEGYPDSQGKSCPLSPANETVSTLSREDSAQPCTDEVVTGRALVCPVCNLEQETSDLTLFNIHVDICLNKGIIQELRNSEVNSVKQPKGSTRSTDRLQKASGKTKRAGRKTKGSTSKRTKPRDPRHTLDGFFK

Target ORF information:

RefSeq Version	NM_138516.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA polymerase kappa (Polk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138516.1

ORF Insert Sequence:

ATGGATAACA CAAAGGAAAA GAACAACTTC AAAGACGCCC TTCTGCTCCG CATGGGACTA 
AATGATAACA AGGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAACTCA AGAAGGAGAA GCAAGTCAAT 
CAACGGATTG AAAACATGAT GCAGCAAAAG GCGCAAATTA CCAGCCAGCA ACTAAGGAAG 
GCCCAGCTAC AGGTTGACAG ATTTGCGATG GGATTAGAAC GGAACCGGAA TTTGAACAAC 
ACCATAGTTC ACGTGGACAT GGATGCTTTC TATGCGGCCG TAGAAATGAG GGACAACCCG 
GAACTGAAGG AGAAGCCCAT CGCTGTAGGA TCCATGAGCA TGCTGGCTAC TTCAAATTAC 
CACGCAAGGA GGTTTGGGGT TCGCGCAGCC ATGCCAGGAT TTATTGCCAA GAGACTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAGTATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TCGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAGGCCTAC 
TTGAATATAA CACAGCACTT ACAGGAAAGG CAAGGTTGGC CTGAGGACAA AAGAAGATAT 
TTCATCAAAA TGGGAAACTG TAGAAAAATT GACACACCCC GGCAGGAAGC TGATGAGCTG 
ACTGAGCGGG AGCGCTCCAT CTCCCCACTG CTTTTTGAAG ATAGCCCTCC TGATTTGCAA 
CCCCAAGGAA GTCCTTTCCA ACTGAACTCT GAAGAACAAA ACAATCCTCA AATAGCCCAA 
AATCCAGTTG TTTTTGGAAC ATCAGCTGAG GAAGTGGTAA AGGAAATTCG CTTCAGAATT 
GAACAGAAAA CAACGCTAAC AGCCAGCGCA GGCATCGCCC CTAATACAAT GTTAGCAAAA 
GTGTGCAGTG ATAAGAATAA ACCAAATGGA CAGTACCAGA TCGTCCCCAG CAGGAGCGCA 
GTGATGGACT TCATCAAGGA CTTGCCTATT CGAAAGGTTT CTGGAATAGG AAAAGTTACG 
GAGAAAATGT TGCTGGCTCT CGGGATTGTT ACTTGCACAG AACTCTACCA ACAGAGAGCG 
TTGCTTTCTC TCCTTTTCTC GGAGTCATCC TGGCACTATT TTCTTCATGT TGCACTGGGC 
CTGGGTTCAA CAGACCTGGC GAGGGATGGA GAAAGGAAAA GTATGAGCGT TGAAAGGACA 
TTTAGTGAGA TCAATAAAAC AGAGGAACAG TACAGCTTGT GCCAAGAACT GTGCACTGAG 
CTCGCCCACG ATCTCCAGAA GGAAGGACTT AAGGGCAGAA CTGTCACCAT CAAGCTGAAG 
AATGTGGATT TTGAAGTAAA AACCCGCGCA TCTACAGTAC CGTCCGCCAT TTCTACTGCA 
GAGGACATAT TTGCCGTTGC CAAGGAGCTG CTAAGGACAG AAGTCACTGC AGGCTCTCCA 
CAGCCCCTGC GGCTAAGACT GATGGGTGTT CGGATGTCTA CTTTCTCCAA CGACGATGAC 
AGGAAGCACC AACAAAGGAG CATCATTGGC TTCTTACAAG CTGGAAACCA AGGTTTGTCA 
CCCACTGGGT GTACTGCAGA CAGAACTGAC AAAACTGAGC TCGTAAAGCC CTTAGAAACG 
TCTCGTAAGA AGAGTTTCTT TGATAAAAAG CGATCAGAAA GGATCCCGAA CTGTCAAGAC 
CCACCCAGAT GTAAAATCGC GGGTGAGCAA GCTTTACAGG CCTCGGAACC ATCCCAAGCG 
TTGAAGAAGA TGAGCGAGAG TTTCAAAACC TCAGAGAATT CAAATGACCC TCAGACATTT 
ATATGTCCAG TTTGCTTTAG GGAGCAAGAA GGCATCAGTC TGGAAGCCTT TAATGAACAT 
GTAGATGCAT GTCTCGATGG ACCGTCCACC AGTGAGAACT CAGAGATGCC CAGTTACTCA 
CATGCTTCGT CTGCGGACAT TGGTCAGCAG GCACACCGTG CTGTTCCACT GTGGGAGAAA 
CGGGGGCATG GAGATGGAGA GATCACCTCA GGAGATGGTG CAGATCTAAC AGAGACTGAA 
GACAGATCGC CGAAAGCAGC AAGCATGGAC GCTGTAGGGA ATAATCACAG CGAAGGGGAG 
GGATATCCTG ATAGTCAGGG CAAGTCCTGC CCTCTGTCAC CCGCAAACGA AACTGTCAGC 
ACGTTAAGTA GGGAAGACTC TGCCCAGCCC TGTACAGATG AGGTAGTGAC AGGACGAGCT 
CTAGTTTGTC CCGTTTGTAA CCTAGAACAA GAGACGTCTG ATCTCACCCT CTTCAACATC 
CACGTGGACA TTTGCTTAAA TAAAGGCATC ATCCAAGAAC TGAGAAACAG CGAAGTTAAT 
TCAGTTAAGC AACCCAAAGG GAGCACGAGA AGCACTGACA GACTTCAGAA GGCTTCAGGA 
AAGACAAAAA GGGCAGGCCG GAAGACAAAG GGCTCAACAT CGAAGAGAAC AAAACCCCGA 
GATCCCAGAC ACACCCTTGA TGGATTTTTT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA m
Nature543(7646)573-576(2017 03)
Xiang Y,Laurent B,Hsu CH,Nachtergaele S,Lu Z,Sheng W,Xu C,Chen H,Ouyang J,Wang S,Ling D,Hsu PH,Zou L,Jambhekar A,He C,Shi Y

DNA polymerases and repair synthesis in NER in human cells.
DNA repair10(7)730-3(2011 Jul)
Lehmann AR

Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells.
Molecular cell37(5)714-27(2010 Mar)
Ogi T,Limsirichaikul S,Overmeer RM,Volker M,Takenaka K,Cloney R,Nakazawa Y,Niimi A,Miki Y,Jaspers NG,Mullenders LH,Yamashita S,Fousteri MI,Lehmann AR

DNA polymerase kappa deficiency does not affect somatic hypermutation in mice.
European journal of immunology32(11)3152-60(2002 Nov)
Schenten D,Gerlach VL,Guo C,Velasco-Miguel S,Hladik CL,White CL,Friedberg EC,Rajewsky K,Esposito G

The absence of Mth1 inactivation and DNA polymerase kappa overexpression in rat mammary carcinomas with frequent A:T to C:G transversions.
Japanese journal of cancer research : Gann93(5)501-6(2002 May)
Okochi E,Ichimura S,Sugimura T,Ushijima T