Gpc2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpc2
Entrez Gene ID	171517
Full Name	glypican 2
General protein information	Preferred Names glypican 2 Names glypican-2 HSPG M13 cerebroglycan
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q11
Summary	heparan sulfate proteoglycan; may play a role with neural development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138511.1	NP_612520.1	glypican-2 precursor

Pathways from BioSystems

Homologies

	GPC2	XP_001103286.1
Pan troglodytes (chimpanzee)	GPC2	XP_527835.3
Mus musculus (house mouse)	Gpc2	NP_766000.1
Homo sapiens (human)	GPC2	NP_689955.1
Canis lupus familiaris (dog)	GPC2	XP_005621114.1
Bos taurus (cattle)	GPC2	NP_001180109.1
Rattus norvegicus (Norway rat)	Gpc2	NP_612520.1

Gene Ontology
Bibliography

Related articles in PubMed

HB-GAM (pleiotrophin) reverses inhibition of neural regeneration by the CNS extracellular matrix.
Paveliev M, Fenrich KK, Kislin M, Kuja-Panula J, Kulesskiy E, Varjosalo M, Kajander T, Mugantseva E, Ahonen-Bishopp A, Khiroug L, Kulesskaya N, Rougon G, Rauvala H
Scientific reports633916(2016 Sep)

Dally-like core protein and its mammalian homologues mediate stimulatory and inhibitory effects on Hedgehog signal response.
Williams EH, Pappano WN, Saunders AM, Kim MS, Leahy DJ, Beachy PA
Proceedings of the National Academy of Sciences of the United States of America107(13)5869-74(2010 Mar)

Glypican-2 binds to midkine: the role of glypican-2 in neuronal cell adhesion and neurite outgrowth.
Kurosawa N, Chen GY, Kadomatsu K, Ikematsu S, Sakuma S, Muramatsu T
Glycoconjugate journal18(6)499-507(2001 Jun)

Cerebroglycan: an integral membrane heparan sulfate proteoglycan that is unique to the developing nervous system and expressed specifically during neuronal differentiation.
Stipp CS, Litwack ED, Lander AD
The Journal of cell biology124(1-2)149-60(1994 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gpc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01496	NM_138511.1 Latest version!	Rattus norvegicus glypican 2 (Gpc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpc2 ( NM_138511.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_612520.1 Rattus norvegicus glypican 2 (Gpc2), mRNA.

Price & Availability↑

CloneID	ORa01496
Clone ID Related Accession (Same CDS sequence)	NM_138511.1
Accession Version	NM_138511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	glypican-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L20468.1. On Dec 17, 2003 this sequence version replaced XM_346426.1. ##Evidence-Data-START## Transcript exon combination :: L20468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGCGG TGCGACCTCT CCTGCTTTTG CTGCTCCCTC TGTGTCCCGG TCCAGGACCG 
GGACATGGGA GTGAAGCAAA GGTCGTCCGG AGTTGTGCAG AGACTCGGCA GGTGCTGGGG 
GCCCGGGGAT ATAGCTTAAA CCTTATCCCT CCCTCCCTCA TCTCAGGTGA GCACCTTCAG 
ATCTGTCCTC AGGAGTACAC CTGCTGTTCC AGTGAGACAG AGCAGAAGTT GATCCGGGAT 
GCTGAGGTCA CCTTCCGTGG CCTGGTGGAA GACAGTGGCT CCTTCCTGAT TCACACGCTG 
GCTGCCCGCC ACAGAAAGTT TAACGAGTTT TTTCGGGAGA TGCTGTCTAT ATCCCAGCAT 
TCTTTGGCCC AGCTCTTCTC GCATTCCTAT GGTCGCCTGT ATTCCCAGCA TGCCGTCATC 
TTCAATAGCC TGTTCTCCGG CCTGCGGGAC TACTATGAGA AGTCTGGTGA GGGGTTAGAT 
GACACCTTGG CAGACTTCTG GGCCCAGCTC CTGGAGAGAG CCTTCCCTTT GCTGCACCCA 
CAATACAGTT TCCCTCCTGA TTTCCTGCTC TGCCTTACTC GGCTCACTTC AACTGCTGAT 
GGCTCTCTCC AGCCCTTCGG GGACTCGCCC CGCCGCCTCC GCCTACAGAT AACCCGGGCA 
CTGGTGGCAG CCCGGGCCTT GGTCCAGGGT CTGGAGACCG GAAGAAATGT GGTCAGCGAA 
GCACTTAAGG TACCCATGTT GGAAGGCTGC AGACAGGCCC TCATGCGTCT GATCGGCTGC 
CCACTTTGTC GGGGAGTACC CTCGCTTATG CCCTGCCGGG GCTTCTGCCT CAATGTGGCC 
CACGGCTGTC TCAGCAGCAG GGGATTGGAG CCTGAATGGG GCGGATATCT GGATGGTCTC 
CTGCTGCTGG CTGAGAAACT CCAGGGACCC TTTTCCTTCG AGCTGGCTGC TGAGTCCATA 
GGGGTGAAGA TCTCAGAAGG TTTGATGCAC TTGCAGGAAA ACAGTGTAAA GGTGTCAGCC 
AAGGTGTTTC AGGAATGTGG GACGCCTCAC CCGGTGCAAT CCCGTAACCG GCGAGCACCA 
GCACCCCGGG AAGAGACTAG CCGGTCTTGG AGGTCGTCAG CTGAGGAAGA ACGCCCCACA 
ACAGCGGCAG GCACTAATCT GCACCGTCTG GTTTGGGAGC TCCGAGAGAG GCTTAGCCGA 
GTGCGGGGCT TCTGGGCTGG ACTTCCCGTT ACGGTGTGTG GGGACTCCCG CATGGCGGCA 
GATCTCTCCC AGGAGGCGGC ACCCTGCTGG ACTGGGGTTG GGAGAGGCCG GTATATGTCG 
CCTGTGGTCG TGGGCTCCTT GAACGAGCAG CTCCATAACC CGGAGTTGGA TACTTCGAGT 
CCCGATGTCC CGACACGTCG GCGGAGGCTC CATCTCCGAG CAGCTACTGC TCGAATGAAG 
GCGGCTGCGC TGGGCCAGGA CCTTGACATG CATGATGCGG ATGAAGACGC CAGTGGCTCC 
GGAGGGGGAC AGCAGTACGC AGACGACTGG AAAGCTGGGG CAGCGCCTGT GGTTCCCCCA 
GCCAGGCCTC CAAGGCCACC TCGTCCTCCT CGAAGGGATG GTCTTGGAGT GAGAGGAGGA 
AGTGGCAGTG CCAGATACAA CCAGGGCCGA AGCAGGAATC TGGGGTCCTC TGTTGGCCTC 
CATGCCCCAC GTGTCTTCAT TCTCCTCCCC TCAGCCCTGA CCCTGCTTGG ACTTCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612520.1
CDS	236..1975
Translation	MSAVRPLLLLLLPLCPGPGPGHGSEAKVVRSCAETRQVLGARGYSLNLIPPSLISGEHLQICPQEYTCCSSETEQKLIRDAEVTFRGLVEDSGSFLIHTLAARHRKFNEFFREMLSISQHSLAQLFSHSYGRLYSQHAVIFNSLFSGLRDYYEKSGEGLDDTLADFWAQLLERAFPLLHPQYSFPPDFLLCLTRLTSTADGSLQPFGDSPRRLRLQITRALVAARALVQGLETGRNVVSEALKVPMLEGCRQALMRLIGCPLCRGVPSLMPCRGFCLNVAHGCLSSRGLEPEWGGYLDGLLLLAEKLQGPFSFELAAESIGVKISEGLMHLQENSVKVSAKVFQECGTPHPVQSRNRRAPAPREETSRSWRSSAEEERPTTAAGTNLHRLVWELRERLSRVRGFWAGLPVTVCGDSRMAADLSQEAAPCWTGVGRGRYMSPVVVGSLNEQLHNPELDTSSPDVPTRRRRLHLRAATARMKAAALGQDLDMHDADEDASGSGGGQQYADDWKAGAAPVVPPARPPRPPRPPRRDGLGVRGGSGSARYNQGRSRNLGSSVGLHAPRVFILLPSALTLLGLR

Target ORF information:

RefSeq Version	NM_138511.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glypican 2 (Gpc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138511.1

ORF Insert Sequence:

ATGTCCGCGG TGCGACCTCT CCTGCTTTTG CTGCTCCCTC TGTGTCCCGG TCCAGGACCG 
GGACATGGGA GTGAAGCAAA GGTCGTCCGG AGTTGTGCAG AGACTCGGCA GGTGCTGGGG 
GCCCGGGGAT ATAGCTTAAA CCTTATCCCT CCCTCCCTCA TCTCAGGTGA GCACCTTCAG 
ATCTGTCCTC AGGAGTACAC CTGCTGTTCC AGTGAGACAG AGCAGAAGTT GATCCGGGAT 
GCTGAGGTCA CCTTCCGTGG CCTGGTGGAA GACAGTGGCT CCTTCCTGAT TCACACGCTG 
GCTGCCCGCC ACAGAAAGTT TAACGAGTTT TTTCGGGAGA TGCTGTCTAT ATCCCAGCAT 
TCTTTGGCCC AGCTCTTCTC GCATTCCTAT GGTCGCCTGT ATTCCCAGCA TGCCGTCATC 
TTCAATAGCC TGTTCTCCGG CCTGCGGGAC TACTATGAGA AGTCTGGTGA GGGGTTAGAT 
GACACCTTGG CAGACTTCTG GGCCCAGCTC CTGGAGAGAG CCTTCCCTTT GCTGCACCCA 
CAATACAGTT TCCCTCCTGA TTTCCTGCTC TGCCTTACTC GGCTCACTTC AACTGCTGAT 
GGCTCTCTCC AGCCCTTCGG GGACTCGCCC CGCCGCCTCC GCCTACAGAT AACCCGGGCA 
CTGGTGGCAG CCCGGGCCTT GGTCCAGGGT CTGGAGACCG GAAGAAATGT GGTCAGCGAA 
GCACTTAAGG TACCCATGTT GGAAGGCTGC AGACAGGCCC TCATGCGTCT GATCGGCTGC 
CCACTTTGTC GGGGAGTACC CTCGCTTATG CCCTGCCGGG GCTTCTGCCT CAATGTGGCC 
CACGGCTGTC TCAGCAGCAG GGGATTGGAG CCTGAATGGG GCGGATATCT GGATGGTCTC 
CTGCTGCTGG CTGAGAAACT CCAGGGACCC TTTTCCTTCG AGCTGGCTGC TGAGTCCATA 
GGGGTGAAGA TCTCAGAAGG TTTGATGCAC TTGCAGGAAA ACAGTGTAAA GGTGTCAGCC 
AAGGTGTTTC AGGAATGTGG GACGCCTCAC CCGGTGCAAT CCCGTAACCG GCGAGCACCA 
GCACCCCGGG AAGAGACTAG CCGGTCTTGG AGGTCGTCAG CTGAGGAAGA ACGCCCCACA 
ACAGCGGCAG GCACTAATCT GCACCGTCTG GTTTGGGAGC TCCGAGAGAG GCTTAGCCGA 
GTGCGGGGCT TCTGGGCTGG ACTTCCCGTT ACGGTGTGTG GGGACTCCCG CATGGCGGCA 
GATCTCTCCC AGGAGGCGGC ACCCTGCTGG ACTGGGGTTG GGAGAGGCCG GTATATGTCG 
CCTGTGGTCG TGGGCTCCTT GAACGAGCAG CTCCATAACC CGGAGTTGGA TACTTCGAGT 
CCCGATGTCC CGACACGTCG GCGGAGGCTC CATCTCCGAG CAGCTACTGC TCGAATGAAG 
GCGGCTGCGC TGGGCCAGGA CCTTGACATG CATGATGCGG ATGAAGACGC CAGTGGCTCC 
GGAGGGGGAC AGCAGTACGC AGACGACTGG AAAGCTGGGG CAGCGCCTGT GGTTCCCCCA 
GCCAGGCCTC CAAGGCCACC TCGTCCTCCT CGAAGGGATG GTCTTGGAGT GAGAGGAGGA 
AGTGGCAGTG CCAGATACAA CCAGGGCCGA AGCAGGAATC TGGGGTCCTC TGTTGGCCTC 
CATGCCCCAC GTGTCTTCAT TCTCCTCCCC TCAGCCCTGA CCCTGCTTGG ACTTCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HB-GAM (pleiotrophin) reverses inhibition of neural regeneration by the CNS extracellular matrix.
Scientific reports633916(2016 Sep)
Paveliev M,Fenrich KK,Kislin M,Kuja-Panula J,Kulesskiy E,Varjosalo M,Kajander T,Mugantseva E,Ahonen-Bishopp A,Khiroug L,Kulesskaya N,Rougon G,Rauvala H

Dally-like core protein and its mammalian homologues mediate stimulatory and inhibitory effects on Hedgehog signal response.
Proceedings of the National Academy of Sciences of the United States of America107(13)5869-74(2010 Mar)
Williams EH,Pappano WN,Saunders AM,Kim MS,Leahy DJ,Beachy PA

Glypican-2 binds to midkine: the role of glypican-2 in neuronal cell adhesion and neurite outgrowth.
Glycoconjugate journal18(6)499-507(2001 Jun)
Kurosawa N,Chen GY,Kadomatsu K,Ikematsu S,Sakuma S,Muramatsu T

Cerebroglycan: an integral membrane heparan sulfate proteoglycan that is unique to the developing nervous system and expressed specifically during neuronal differentiation.
The Journal of cell biology124(1-2)149-60(1994 Jan)
Stipp CS,Litwack ED,Lander AD