Gchfr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gchfr
Entrez Gene ID	171128
Full Name	GTP cyclohydrolase I feedback regulator
General protein information	Preferred Names GTP cyclohydrolase I feedback regulator Names GTP cyclohydrolase 1 feedback regulatory protein GFRP GTP cyclohydrolase I feedback regulatory protein p35
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35
Summary	regulates activity of GTP cyclohydrolase I (Gch); may be involved with phenylalanine metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133595.2	NP_598279.1	GTP cyclohydrolase 1 feedback regulatory protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GCHFR	NP_005249.1
Canis lupus familiaris (dog)	GCHFR	XP_544622.2
Mus musculus (house mouse)	Gchfr	NP_796131.1
Gallus gallus (chicken)	GCHFR	NP_001186451.1
Pan troglodytes (chimpanzee)	GCHFR	XP_001146591.1
Xenopus tropicalis (tropical clawed frog)	gchfr	NP_998880.1
	GCHFR	XP_001097382.2
Bos taurus (cattle)	GCHFR	NP_001107998.1
Rattus norvegicus (Norway rat)	Gchfr	NP_598279.1
Danio rerio (zebrafish)	gchfr	NP_957056.1
Caenorhabditis elegans (roundworm)	gfrp-1	NP_001041021.2

Gene Ontology
Bibliography

Related articles in PubMed

The N-terminal peptide of mammalian GTP cyclohydrolase I is an autoinhibitory control element and contributes to binding the allosteric regulatory protein GFRP.
Higgins CE, Gross SS
The Journal of biological chemistry286(14)11919-28(2011 Apr)

GTP cyclohydrolase feedback regulatory protein controls cofactor 6-tetrahydrobiopterin synthesis in the cytosol and in the nucleus of epidermal keratinocytes and melanocytes.
Chavan B, Gillbro JM, Rokos H, Schallreuter KU
The Journal of investigative dermatology126(11)2481-9(2006 Nov)

Changes in tetrahydrobiopterin levels in endothelial cells and adult cardiomyocytes induced by LPS and hydrogen peroxide--a role for GFRP?
Kalivendi S, Hatakeyama K, Whitsett J, Konorev E, Kalyanaraman B, V?squez-Vivar J
Free radical biology & medicine38(4)481-91(2005 Feb)

Structural basis of biopterin-induced inhibition of GTP cyclohydrolase I by GFRP, its feedback regulatory protein.
Maita N, Hatakeyama K, Okada K, Hakoshima T
The Journal of biological chemistry279(49)51534-40(2004 Dec)

The mechanism of potent GTP cyclohydrolase I inhibition by 2,4-diamino-6-hydroxypyrimidine: requirement of the GTP cyclohydrolase I feedback regulatory protein.
Kolinsky MA, Gross SS
The Journal of biological chemistry279(39)40677-82(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The N-terminal peptide of mammalian GTP cyclohydrolase I is an autoinhibitory control element and contributes to binding the allosteric regulatory protein GFRP.
Title: The N-terminal peptide of mammalian GTP cyclohydrolase I is an autoinhibitory control element and contributes to binding the allosteric regulatory protein GFRP.

allosteric regulation of GTPCH-I activity in the cardiovascular system maybe an important mechanism regulating BH4 levels through GTPCH-I feedback regulatory protein signaling
Title: Changes in tetrahydrobiopterin levels in endothelial cells and adult cardiomyocytes induced by LPS and hydrogen peroxide--a role for GFRP?

conformational analysis of GTP cyclohydrolase I binding GFRP, its feedback regulatory protein
Title: Structural basis of biopterin-induced inhibition of GTP cyclohydrolase I by GFRP, its feedback regulatory protein.

GTPCH feedback regulatory protein has a role in GTCPCH inhibition by DAHP (2,4-diamino-6-hydroxypyrimidine)
Title: The mechanism of potent GTP cyclohydrolase I inhibition by 2,4-diamino-6-hydroxypyrimidine: requirement of the GTP cyclohydrolase I feedback regulatory protein.

The following Gchfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gchfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01030	NM_133595.2 Latest version!	Rattus norvegicus GTP cyclohydrolase I feedback regulator (Gchfr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gchfr ( NM_133595.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598279.1 Rattus norvegicus GTP cyclohydrolase I feedback regulator (Gchfr), mRNA.

Price & Availability↑

CloneID	ORa01030
Clone ID Related Accession (Same CDS sequence)	NM_133595.2
Accession Version	NM_133595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	GTP cyclohydrolase 1 feedback regulatory protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U53710.1. On Dec 23, 2009 this sequence version replaced NM_133595.1. ##Evidence-Data-START## Transcript exon combination :: U53710.1, U85512.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTACC TGCTCATCAG CACTCAGATC CGTATGGAAG TGGGTCCCAC CATGGTGGGT 
GATGAGCACT CGGATCCAGA GCTGATGCAG CAGTTGGGGG CCTCCAAGAG ACGAGTCCTG 
GGAAACAACT TTTATGAATA CTACGTCAAT GACCCTCCTC GAATAGTCCT GGACAAGCTG 
GAATGCAGGG GCTTCCGAGT GTTAAGCATG ACAGGGGTGG GCCAGACGCT GGTGTGGTGT 
CTGCACAAGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598279.1
CDS	41..295
Translation	MPYLLISTQIRMEVGPTMVGDEHSDPELMQQLGASKRRVLGNNFYEYYVNDPPRIVLDKLECRGFRVLSMTGVGQTLVWCLHKE

Target ORF information:

RefSeq Version	NM_133595.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GTP cyclohydrolase I feedback regulator (Gchfr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133595.2

ORF Insert Sequence:

ATGCCCTACC TGCTCATCAG CACTCAGATC CGTATGGAAG TGGGTCCCAC CATGGTGGGT 
GATGAGCACT CGGATCCAGA GCTGATGCAG CAGTTGGGGG CCTCCAAGAG ACGAGTCCTG 
GGAAACAACT TTTATGAATA CTACGTCAAT GACCCTCCTC GAATAGTCCT GGACAAGCTG 
GAATGCAGGG GCTTCCGAGT GTTAAGCATG ACAGGGGTGG GCCAGACGCT GGTGTGGTGT 
CTGCACAAGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The N-terminal peptide of mammalian GTP cyclohydrolase I is an autoinhibitory control element and contributes to binding the allosteric regulatory protein GFRP.
The Journal of biological chemistry286(14)11919-28(2011 Apr)
Higgins CE,Gross SS

GTP cyclohydrolase feedback regulatory protein controls cofactor 6-tetrahydrobiopterin synthesis in the cytosol and in the nucleus of epidermal keratinocytes and melanocytes.
The Journal of investigative dermatology126(11)2481-9(2006 Nov)
Chavan B,Gillbro JM,Rokos H,Schallreuter KU

Changes in tetrahydrobiopterin levels in endothelial cells and adult cardiomyocytes induced by LPS and hydrogen peroxide--a role for GFRP?
Free radical biology & medicine38(4)481-91(2005 Feb)
Kalivendi S,Hatakeyama K,Whitsett J,Konorev E,Kalyanaraman B,V?squez-Vivar J

Structural basis of biopterin-induced inhibition of GTP cyclohydrolase I by GFRP, its feedback regulatory protein.
The Journal of biological chemistry279(49)51534-40(2004 Dec)
Maita N,Hatakeyama K,Okada K,Hakoshima T

The mechanism of potent GTP cyclohydrolase I inhibition by 2,4-diamino-6-hydroxypyrimidine: requirement of the GTP cyclohydrolase I feedback regulatory protein.
The Journal of biological chemistry279(39)40677-82(2004 Sep)
Kolinsky MA,Gross SS