Wfdc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wfdc1
Entrez Gene ID	171112
Full Name	WAP four-disulfide core domain 1
Synonyms	ps20
General protein information	Preferred Names WAP four-disulfide core domain 1 Names WAP four-disulfide core domain protein 1 20-kDa prostate stromal protein prostate stromal protein ps20 ps20 growth inhibitor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	involved in the regulation of growth [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255705.2	XP_006255767.1	WAP four-disulfide core domain protein 1 isoform X1
NM_133581.1	NP_598265.1	WAP four-disulfide core domain protein 1 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wfdc1	NP_075884.1
Canis lupus familiaris (dog)	WFDC1	XP_005620699.1
Bos taurus (cattle)	WFDC1	XP_005218473.1
Pan troglodytes (chimpanzee)	WFDC1	XP_511141.2
Homo sapiens (human)	WFDC1	NP_067020.2
Rattus norvegicus (Norway rat)	Wfdc1	NP_598265.1

Gene Ontology
Bibliography

Related articles in PubMed

WFDC1 is a key modulator of inflammatory and wound repair responses.
Ressler SJ, Dang TD, Wu SM, Tse DY, Gilbert BE, Vyakarnam A, Yang F, Schauer IG, Barron DA, Rowley DR
The American journal of pathology184(11)2951-64(2014 Nov)

Suppression of ps20 expression in the rat uterus by tamoxifen and estrogens.
Hung H
Endocrinology146(5)2388-96(2005 May)

Decreased stromal expression and increased epithelial expression of WFDC1/ps20 in prostate cancer is associated with reduced recurrence-free survival.
McAlhany SJ, Ayala GE, Frolov A, Ressler SJ, Wheeler TM, Watson JE, Collins C, Rowley DR
The Prostate61(2)182-91(2004 Oct)

Molecular analysis of WFDC1/ps20 gene in prostate cancer.
Watson JE, Kamkar S, James K, Kowbel D, Andaya A, Paris PL, Simko J, Carroll P, McAlhany S, Rowley D, Collins C
The Prostate61(2)192-9(2004 Oct)

Molecular cloning and expression of ps20 growth inhibitor. A novel WAP-type "four-disulfide core" domain protein expressed in smooth muscle.
Larsen M, Ressler SJ, Lu B, Gerdes MJ, McBride L, Dang TD, Rowley DR
The Journal of biological chemistry273(8)4574-84(1998 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data indicate that ps20 protein uterine expression is negatively regulated by estrogens and tamoxifen and suggest that ps20 may function as a mediator of local growth
Title: Suppression of ps20 expression in the rat uterus by tamoxifen and estrogens.

The following Wfdc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wfdc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01000	NM_133581.1 Latest version!	Rattus norvegicus WAP four-disulfide core domain 1 (Wfdc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa01000	XM_006255705.2 Latest version!	Rattus norvegicus WAP four-disulfide core domain 1 (Wfdc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wfdc1 ( NM_133581.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598265.1 Rattus norvegicus WAP four-disulfide core domain 1 (Wfdc1), mRNA.

Price & Availability↑

CloneID	ORa01000
Clone ID Related Accession (Same CDS sequence)	XM_006255705.2 , NM_133581.1
Accession Version	NM_133581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	WAP four-disulfide core domain protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF037272.1. ##Evidence-Data-START## Transcript exon combination :: AF037272.1, FQ219846.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGTAGCT GCGACAGGAA AGCCCTCTGG GCTCTGAGCT TCCTACTGCT GCTACTGGGC 
TCCAGCTCTG TTCAGGGCAC TTGGGAGGCA ATGTTGCCGG TCAGGCTGGC TGAGAAGTCC 
CAAGCTGAAG AGGTTGCAGC AACAGGCTCC CGGCAGCCCC ACGCAGACCG CTGCCCACCA 
CCGCCACGGA CGCTACCCCC GGGTGCCTGT CAGGCCACAC GCTGCCAGTC TGACTCTGAG 
TGCCCACGAC ACAGACGCTG CTGTTACAAC GGCTGTGCCT ATGCCTGCCT GGAGGCGGTG 
CCACCGCCAC CAGTTCTAGA CTGGCTGGTG CAGCCCAAAC CACGGTGGCT TGGTGGCAAT 
GGCTGGCTGC TGGATGGTCC GGAGGAAGTG TTACAAGCAG AGGCCTGCAG CACCACTGAG 
GATGGGGCAG AGCCACTCCT CTGTCCCTCA GGCTATGAGT GCCACATCCT GCAGCCAGGG 
GATGCGGCCC AGGGCATACC CAACCATGGA CGGTGTGTTA AGCAACGTCG ACAAGCAGAG 
GGGCGGGTCC TGCGACAGAA GCTTCACAAG GAGTACCCAG AAGGTGACTC CAAGTATGTG 
GCAGAGCCTG GGAAGGGACA ACAGAGGCAC TTTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598265.1
CDS	52..690
Translation	MGSCDRKALWALSFLLLLLGSSSVQGTWEAMLPVRLAEKSQAEEVAATGSRQPHADRCPPPPRTLPPGACQATRCQSDSECPRHRRCCYNGCAYACLEAVPPPPVLDWLVQPKPRWLGGNGWLLDGPEEVLQAEACSTTEDGAEPLLCPSGYECHILQPGDAAQGIPNHGRCVKQRRQAEGRVLRQKLHKEYPEGDSKYVAEPGKGQQRHFP

Target ORF information:

RefSeq Version	NM_133581.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WAP four-disulfide core domain 1 (Wfdc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133581.1

ORF Insert Sequence:

ATGGGTAGCT GCGACAGGAA AGCCCTCTGG GCTCTGAGCT TCCTACTGCT GCTACTGGGC 
TCCAGCTCTG TTCAGGGCAC TTGGGAGGCA ATGTTGCCGG TCAGGCTGGC TGAGAAGTCC 
CAAGCTGAAG AGGTTGCAGC AACAGGCTCC CGGCAGCCCC ACGCAGACCG CTGCCCACCA 
CCGCCACGGA CGCTACCCCC GGGTGCCTGT CAGGCCACAC GCTGCCAGTC TGACTCTGAG 
TGCCCACGAC ACAGACGCTG CTGTTACAAC GGCTGTGCCT ATGCCTGCCT GGAGGCGGTG 
CCACCGCCAC CAGTTCTAGA CTGGCTGGTG CAGCCCAAAC CACGGTGGCT TGGTGGCAAT 
GGCTGGCTGC TGGATGGTCC GGAGGAAGTG TTACAAGCAG AGGCCTGCAG CACCACTGAG 
GATGGGGCAG AGCCACTCCT CTGTCCCTCA GGCTATGAGT GCCACATCCT GCAGCCAGGG 
GATGCGGCCC AGGGCATACC CAACCATGGA CGGTGTGTTA AGCAACGTCG ACAAGCAGAG 
GGGCGGGTCC TGCGACAGAA GCTTCACAAG GAGTACCCAG AAGGTGACTC CAAGTATGTG 
GCAGAGCCTG GGAAGGGACA ACAGAGGCAC TTTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wfdc1 ( XM_006255705.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255767.1 Rattus norvegicus WAP four-disulfide core domain 1 (Wfdc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01000
Clone ID Related Accession (Same CDS sequence)	XM_006255705.2 , NM_133581.1
Accession Version	XM_006255705.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	WAP four-disulfide core domain protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006255705.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGCT GCGACAGGAA AGCCCTCTGG GCTCTGAGCT TCCTACTGCT GCTACTGGGC 
TCCAGCTCTG TTCAGGGCAC TTGGGAGGCA ATGTTGCCGG TCAGGCTGGC TGAGAAGTCC 
CAAGCTGAAG AGGTTGCAGC AACAGGCTCC CGGCAGCCCC ACGCAGACCG CTGCCCACCA 
CCGCCACGGA CGCTACCCCC GGGTGCCTGT CAGGCCACAC GCTGCCAGTC TGACTCTGAG 
TGCCCACGAC ACAGACGCTG CTGTTACAAC GGCTGTGCCT ATGCCTGCCT GGAGGCGGTG 
CCACCGCCAC CAGTTCTAGA CTGGCTGGTG CAGCCCAAAC CACGGTGGCT TGGTGGCAAT 
GGCTGGCTGC TGGATGGTCC GGAGGAAGTG TTACAAGCAG AGGCCTGCAG CACCACTGAG 
GATGGGGCAG AGCCACTCCT CTGTCCCTCA GGCTATGAGT GCCACATCCT GCAGCCAGGG 
GATGCGGCCC AGGGCATACC CAACCATGGA CGGTGTGTTA AGCAACGTCG ACAAGCAGAG 
GGGCGGGTCC TGCGACAGAA GCTTCACAAG GAGTACCCAG AAGGTGACTC CAAGTATGTG 
GCAGAGCCTG GGAAGGGACA ACAGAGGCAC TTTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255767.1
CDS	305..943
Translation	MGSCDRKALWALSFLLLLLGSSSVQGTWEAMLPVRLAEKSQAEEVAATGSRQPHADRCPPPPRTLPPGACQATRCQSDSECPRHRRCCYNGCAYACLEAVPPPPVLDWLVQPKPRWLGGNGWLLDGPEEVLQAEACSTTEDGAEPLLCPSGYECHILQPGDAAQGIPNHGRCVKQRRQAEGRVLRQKLHKEYPEGDSKYVAEPGKGQQRHFP

Target ORF information:

RefSeq Version	XM_006255705.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WAP four-disulfide core domain 1 (Wfdc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255705.2

ORF Insert Sequence:

ATGGGTAGCT GCGACAGGAA AGCCCTCTGG GCTCTGAGCT TCCTACTGCT GCTACTGGGC 
TCCAGCTCTG TTCAGGGCAC TTGGGAGGCA ATGTTGCCGG TCAGGCTGGC TGAGAAGTCC 
CAAGCTGAAG AGGTTGCAGC AACAGGCTCC CGGCAGCCCC ACGCAGACCG CTGCCCACCA 
CCGCCACGGA CGCTACCCCC GGGTGCCTGT CAGGCCACAC GCTGCCAGTC TGACTCTGAG 
TGCCCACGAC ACAGACGCTG CTGTTACAAC GGCTGTGCCT ATGCCTGCCT GGAGGCGGTG 
CCACCGCCAC CAGTTCTAGA CTGGCTGGTG CAGCCCAAAC CACGGTGGCT TGGTGGCAAT 
GGCTGGCTGC TGGATGGTCC GGAGGAAGTG TTACAAGCAG AGGCCTGCAG CACCACTGAG 
GATGGGGCAG AGCCACTCCT CTGTCCCTCA GGCTATGAGT GCCACATCCT GCAGCCAGGG 
GATGCGGCCC AGGGCATACC CAACCATGGA CGGTGTGTTA AGCAACGTCG ACAAGCAGAG 
GGGCGGGTCC TGCGACAGAA GCTTCACAAG GAGTACCCAG AAGGTGACTC CAAGTATGTG 
GCAGAGCCTG GGAAGGGACA ACAGAGGCAC TTTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WFDC1 is a key modulator of inflammatory and wound repair responses.
The American journal of pathology184(11)2951-64(2014 Nov)
Ressler SJ,Dang TD,Wu SM,Tse DY,Gilbert BE,Vyakarnam A,Yang F,Schauer IG,Barron DA,Rowley DR

Suppression of ps20 expression in the rat uterus by tamoxifen and estrogens.
Endocrinology146(5)2388-96(2005 May)
Hung H

Decreased stromal expression and increased epithelial expression of WFDC1/ps20 in prostate cancer is associated with reduced recurrence-free survival.
The Prostate61(2)182-91(2004 Oct)
McAlhany SJ,Ayala GE,Frolov A,Ressler SJ,Wheeler TM,Watson JE,Collins C,Rowley DR

Molecular analysis of WFDC1/ps20 gene in prostate cancer.
The Prostate61(2)192-9(2004 Oct)
Watson JE,Kamkar S,James K,Kowbel D,Andaya A,Paris PL,Simko J,Carroll P,McAlhany S,Rowley D,Collins C

Molecular cloning and expression of ps20 growth inhibitor. A novel WAP-type "four-disulfide core" domain protein expressed in smooth muscle.
The Journal of biological chemistry273(8)4574-84(1998 Feb)
Larsen M,Ressler SJ,Lu B,Gerdes MJ,McBride L,Dang TD,Rowley DR