Necab2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Necab2
Entrez Gene ID	170928
Full Name	N-terminal EF-hand calcium binding protein 2
Synonyms	Efcbp2
General protein information	Preferred Names N-terminal EF-hand calcium binding protein 2 Names N-terminal EF-hand calcium-binding protein 2 EF hand calcium binding protein 2 neuronal calcium binding 2 neuronal calcium-binding protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	may mediate calcium signaling and play a role in neuronal function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255703.3	XP_006255765.1	N-terminal EF-hand calcium-binding protein 2 isoform X2
XM_017601166.1	XP_017456655.1	N-terminal EF-hand calcium-binding protein 2 isoform X1
NM_133415.1	NP_596906.1	N-terminal EF-hand calcium-binding protein 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	NECAB2	NP_061938.2
Bos taurus (cattle)	NECAB2	NP_001092447.1
Danio rerio (zebrafish)	necab2	NP_001025366.1
Gallus gallus (chicken)	NECAB2	XP_414072.1
Xenopus tropicalis (tropical clawed frog)	LOC101731915	XP_004913648.1
Pan troglodytes (chimpanzee)	NECAB2	XP_001150555.1
	LOC718169	XP_001110489.2
Mus musculus (house mouse)	Necab2	NP_473436.1
Rattus norvegicus (Norway rat)	Necab2	NP_596906.1
Canis lupus familiaris (dog)	NECAB2	XP_005620942.1

Gene Ontology
Bibliography

Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

The association of metabotropic glutamate receptor type 5 with the neuronal Ca2+-binding protein 2 modulates receptor function.
Canela L, Fern?ndez-Due?as V, Albergaria C, Watanabe M, Llu?s C, Mallol J, Canela EI, Franco R, Luj?n R, Ciruela F
Journal of neurochemistry111(2)555-67(2009 Oct)

The neuronal Ca(2+) -binding protein 2 (NECAB2) interacts with the adenosine A(2A) receptor and modulates the cell surface expression and function of the receptor.
Canela L, Luj?n R, Llu?s C, Burgue?o J, Mallol J, Canela EI, Franco R, Ciruela F
Molecular and cellular neurosciences36(1)1-12(2007 Sep)

NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern.
Sugita S, Ho A, S?dhof TC
Neuroscience112(1)51-63(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NECAB2 by its physical interaction with mGlu(5b) receptor modulates receptor function
Title: The association of metabotropic glutamate receptor type 5 with the neuronal Ca2+-binding protein 2 modulates receptor function.

The following Necab2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Necab2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa38982	XM_006255703.3 Latest version!	Rattus norvegicus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa59356	XM_017601166.1 Latest version!	Rattus norvegicus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa00728	NM_133415.1 Latest version!	Rattus norvegicus N-terminal EF-hand calcium binding protein 2 (Necab2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Necab2 ( XM_006255703.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255765.1 Rattus norvegicus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38982
Clone ID Related Accession (Same CDS sequence)	XM_006255703.3
Accession Version	XM_006255703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N-terminal EF-hand calcium-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGAA GGCTTGGAGC TCCCGTGGCC TTCCGAGCAC GGCTGGGTGC AGCTGTGCGC 
TCAGCATTGG AGGAGTCTAG GGGGATGAGT GTGCGTACAC CTGCAGAGAT GCATGTGGGG 
GTGTGTGAGT GTGTAGCACG AGTGTGTGTG TCTGTCAGCA GCCGTGGGCA GGCTGGCGGC 
GCGGTGGGAA GAGGGATTGA TAGCTTATGG AGGATGGGGA GGGCCTCTGC CTGCTGCAGT 
CTCTCCAACC TGGGTCCCAG CCTGGAGGTC TCCTGGGGAA GTTTGGGCTT CACCCAAGCT 
GTTCCTGGGA GAGGGATCTC AAGCTGGGTT ACGAGGCTGC TCAGGGCACC TGCCCAGACT 
CATCTCAGGA CTGACTCCAG CCCTCCTCTC TCCAGATTTT CCGCCGTGCG GATAAAAACG 
ACGACGGGAA ACTGTCCCTT GAGGAATTTC AACTGTTCTT CGCGGATGGC GTCCTCAATG 
AGAAGGAGCT GGAGGGCCTC TTCCACACCA TTGATTCAGA CAACACCAAA TTACTTTGTG 
GAACACATGG GAGACTACGA AGATGTCCTG GCCTCCCTCG AGACTTTGAA CCACTCAGTC 
CTGAAGGCTA TGGGCTACAC CAAGAAGGTG TATGAAGGTG GGAACAACGT GGACCAGTTT 
GTGACCCGCT TCCTTCTGAA GGAGACGGCC AATCAGATCC AGTCGCTGCT CAGCTCTGTG 
GAAAGCGCCG TGGAAGCCAT CGAAGAACAG ACCAGCCAGA TCCGACAGGA CCACTGCAAA 
CCTAGTCCTG GCGTCAACGA GAGCCGCTAC GGAGGACCCA CTCCTCCCTA CATCCCCAAC 
CACAAGCTTG TGGTCCCGGA ACCAGTGAAG AGCCTGCCTG TGGCCATAGG GGAGCCAAAG 
GAGGAGGGGC TGGAGGTCCA GATAAGCCGG TTGGCAGAGC TGATTGGGAG ACTGGAGAGC 
AAGACCCTCT CGTTTGACCT GCAGCAGCGC CTCTCGGATG AAGAAGGCAC CAACATGTAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCTGAGC AGCTGGGCGA GTTCTTGGAT 
TCCCTGAGAC AGTACCTTCG GAGCACTGCT GAAGAAAGGA ACTGCTTCCA TGTGGCCGCG 
GTGAGGATGG CAGATGGGCT AACCTTCGTC ATCTACGAGT TCTGGGAGAC AGAGGAGGAG 
TGGAAGAGGC ACCTGCAGAG CCCTGTGTGC AAGGCCTTCC GACATGTCAA GGTTGACACA 
CTGAGCCAGC CTGAGGCACT CTCCCAGATC TCCGTGCCAG CTGCCTGGTG CACCTCCGGC 
CGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255765.1
CDS	874..2202
Translation	MVGRLGAPVAFRARLGAAVRSALEESRGMSVRTPAEMHVGVCECVARVCVSVSSRGQAGGAVGRGIDSLWRMGRASACCSLSNLGPSLEVSWGSLGFTQAVPGRGISSWVTRLLRAPAQTHLRTDSSPPLSRFSAVRIKTTTGNCPLRNFNCSSRMASSMRRSWRASSTPLIQTTPNYFVEHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGNNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQIRQDHCKPSPGVNESRYGGPTPPYIPNHKLVVPEPVKSLPVAIGEPKEEGLEVQISRLAELIGRLESKTLSFDLQQRLSDEEGTNMYLQLVRQEMAVCPEQLGEFLDSLRQYLRSTAEERNCFHVAAVRMADGLTFVIYEFWETEEEWKRHLQSPVCKAFRHVKVDTLSQPEALSQISVPAAWCTSGRD

Target ORF information:

RefSeq Version	XM_006255703.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255703.3

ORF Insert Sequence:

ATGGTGGGAA GGCTTGGAGC TCCCGTGGCC TTCCGAGCAC GGCTGGGTGC AGCTGTGCGC 
TCAGCATTGG AGGAGTCTAG GGGGATGAGT GTGCGTACAC CTGCAGAGAT GCATGTGGGG 
GTGTGTGAGT GTGTAGCACG AGTGTGTGTG TCTGTCAGCA GCCGTGGGCA GGCTGGCGGC 
GCGGTGGGAA GAGGGATTGA TAGCTTATGG AGGATGGGGA GGGCCTCTGC CTGCTGCAGT 
CTCTCCAACC TGGGTCCCAG CCTGGAGGTC TCCTGGGGAA GTTTGGGCTT CACCCAAGCT 
GTTCCTGGGA GAGGGATCTC AAGCTGGGTT ACGAGGCTGC TCAGGGCACC TGCCCAGACT 
CATCTCAGGA CTGACTCCAG CCCTCCTCTC TCCAGATTTT CCGCCGTGCG GATAAAAACG 
ACGACGGGAA ACTGTCCCTT GAGGAATTTC AACTGTTCTT CGCGGATGGC GTCCTCAATG 
AGAAGGAGCT GGAGGGCCTC TTCCACACCA TTGATTCAGA CAACACCAAA TTACTTTGTG 
GAACACATGG GAGACTACGA AGATGTCCTG GCCTCCCTCG AGACTTTGAA CCACTCAGTC 
CTGAAGGCTA TGGGCTACAC CAAGAAGGTG TATGAAGGTG GGAACAACGT GGACCAGTTT 
GTGACCCGCT TCCTTCTGAA GGAGACGGCC AATCAGATCC AGTCGCTGCT CAGCTCTGTG 
GAAAGCGCCG TGGAAGCCAT CGAAGAACAG ACCAGCCAGA TCCGACAGGA CCACTGCAAA 
CCTAGTCCTG GCGTCAACGA GAGCCGCTAC GGAGGACCCA CTCCTCCCTA CATCCCCAAC 
CACAAGCTTG TGGTCCCGGA ACCAGTGAAG AGCCTGCCTG TGGCCATAGG GGAGCCAAAG 
GAGGAGGGGC TGGAGGTCCA GATAAGCCGG TTGGCAGAGC TGATTGGGAG ACTGGAGAGC 
AAGACCCTCT CGTTTGACCT GCAGCAGCGC CTCTCGGATG AAGAAGGCAC CAACATGTAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCTGAGC AGCTGGGCGA GTTCTTGGAT 
TCCCTGAGAC AGTACCTTCG GAGCACTGCT GAAGAAAGGA ACTGCTTCCA TGTGGCCGCG 
GTGAGGATGG CAGATGGGCT AACCTTCGTC ATCTACGAGT TCTGGGAGAC AGAGGAGGAG 
TGGAAGAGGC ACCTGCAGAG CCCTGTGTGC AAGGCCTTCC GACATGTCAA GGTTGACACA 
CTGAGCCAGC CTGAGGCACT CTCCCAGATC TCCGTGCCAG CTGCCTGGTG CACCTCCGGC 
CGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Necab2 ( XM_017601166.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456655.1 Rattus norvegicus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa59356
Clone ID Related Accession (Same CDS sequence)	XM_017601166.1
Accession Version	XM_017601166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N-terminal EF-hand calcium-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT CAATGAGAAG GAGCTGGAGG GCCTCTTCCA CACCATTGAT TCAGACAACA 
CCAAATTACT TTGTGGAACA CATGGGAGAC TACGAAGATG TCCTGGCCTC CCTCGAGACT 
TTGAACCACT CAGTCCTGAA GGCTATGGGC TACACCAAGA AGGTGTATGA AGGTGGGAAC 
AACGTGGACC AGTTTGTGAC CCGCTTCCTT CTGAAGGAGA CGGCCAATCA GATCCAGTCG 
CTGCTCAGCT CTGTGGAAAG CGCCGTGGAA GCCATCGAAG AACAGACCAG CCAGATCCGA 
CAGGACCACT GCAAACCTAG TCCTGGCGTC AACGAGAGCC GCTACGGAGG ACCCACTCCT 
CCCTACATCC CCAACCACAA GCTTGTGGTC CCGGAACCAG TGAAGAGCCT GCCTGTGGCC 
ATAGGGGAGC CAAAGGAGGA GGGGCTGGAG GTCCAGATAA GCCGGTTGGC AGAGCTGATT 
GGGAGACTGG AGAGCAAGAC CCTCTCGTTT GACCTGCAGC AGCGCCTCTC GGATGAAGAA 
GGCACCAACA TGTACCTGCA GCTGGTCCGG CAGGAGATGG CCGTGTGCCC TGAGCAGCTG 
GGCGAGTTCT TGGATTCCCT GAGACAGTAC CTTCGGAGCA CTGCTGAAGA AAGGAACTGC 
TTCCATGTGG CCGCGGTGAG GATGGCAGAT GGGCTAACCT TCGTCATCTA CGAGTTCTGG 
GAGACAGAGG AGGAGTGGAA GAGGCACCTG CAGAGCCCTG TGTGCAAGGC CTTCCGACAT 
GTCAAGGTTG ACACACTGAG CCAGCCTGAG GCACTCTCCC AGATCTCCGT GCCAGCTGCC 
TGGTGCACCT CCGGCCGAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456655.1
CDS	133..996
Translation	MASSMRRSWRASSTPLIQTTPNYFVEHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGNNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQIRQDHCKPSPGVNESRYGGPTPPYIPNHKLVVPEPVKSLPVAIGEPKEEGLEVQISRLAELIGRLESKTLSFDLQQRLSDEEGTNMYLQLVRQEMAVCPEQLGEFLDSLRQYLRSTAEERNCFHVAAVRMADGLTFVIYEFWETEEEWKRHLQSPVCKAFRHVKVDTLSQPEALSQISVPAAWCTSGRD

Target ORF information:

RefSeq Version	XM_017601166.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601166.1

ORF Insert Sequence:

ATGGCGTCCT CAATGAGAAG GAGCTGGAGG GCCTCTTCCA CACCATTGAT TCAGACAACA 
CCAAATTACT TTGTGGAACA CATGGGAGAC TACGAAGATG TCCTGGCCTC CCTCGAGACT 
TTGAACCACT CAGTCCTGAA GGCTATGGGC TACACCAAGA AGGTGTATGA AGGTGGGAAC 
AACGTGGACC AGTTTGTGAC CCGCTTCCTT CTGAAGGAGA CGGCCAATCA GATCCAGTCG 
CTGCTCAGCT CTGTGGAAAG CGCCGTGGAA GCCATCGAAG AACAGACCAG CCAGATCCGA 
CAGGACCACT GCAAACCTAG TCCTGGCGTC AACGAGAGCC GCTACGGAGG ACCCACTCCT 
CCCTACATCC CCAACCACAA GCTTGTGGTC CCGGAACCAG TGAAGAGCCT GCCTGTGGCC 
ATAGGGGAGC CAAAGGAGGA GGGGCTGGAG GTCCAGATAA GCCGGTTGGC AGAGCTGATT 
GGGAGACTGG AGAGCAAGAC CCTCTCGTTT GACCTGCAGC AGCGCCTCTC GGATGAAGAA 
GGCACCAACA TGTACCTGCA GCTGGTCCGG CAGGAGATGG CCGTGTGCCC TGAGCAGCTG 
GGCGAGTTCT TGGATTCCCT GAGACAGTAC CTTCGGAGCA CTGCTGAAGA AAGGAACTGC 
TTCCATGTGG CCGCGGTGAG GATGGCAGAT GGGCTAACCT TCGTCATCTA CGAGTTCTGG 
GAGACAGAGG AGGAGTGGAA GAGGCACCTG CAGAGCCCTG TGTGCAAGGC CTTCCGACAT 
GTCAAGGTTG ACACACTGAG CCAGCCTGAG GCACTCTCCC AGATCTCCGT GCCAGCTGCC 
TGGTGCACCT CCGGCCGAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Necab2 ( NM_133415.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_596906.1 Rattus norvegicus N-terminal EF-hand calcium binding protein 2 (Necab2), mRNA.

Price & Availability↑

CloneID	ORa00728
Clone ID Related Accession (Same CDS sequence)	NM_133415.1
Accession Version	NM_133415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	N-terminal EF-hand calcium-binding protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF193757.1. ##Evidence-Data-START## Transcript exon combination :: AF193757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGAAC CCCGCGCACC GCTCGTCCCC GACATCCCCG CCGCGGACCC CGACCCCGGG 
CCCGCCGCAC CGCGAGGGGG CACAGCCGTC ATCCTGGACA TTTTCCGCCG TGCGGATAAA 
AACGACGACG GGAAACTGTC CCTTGAGGAA TTTCAACTGT TCTTCGCGGA TGGCGTCCTC 
AATGAGAAGG AGCTGGAGGG CCTCTTCCAC ACCATTGATT CAGACAACAC CAACCATGTG 
GACACAAAGG AATTGTGTGA TTACTTTGTG GAACACATGG GAGACTACGA AGATGTCCTG 
GCCTCCCTCG AGACTTTGAA CCACTCAGTC CTGAAGGCTA TGGGCTACAC CAAGAAGGTG 
TATGAAGGTG GGAACAACGT GGACCAGTTT GTGACCCGCT TCCTTCTGAA GGAGACGGCC 
AATCAGATCC AGTCGCTGCT CAGCTCTGTG GAAAGCGCCG TGGAAGCCAT CGAAGAACAG 
ACCAGCCAGA TCCGACAGGA CTACTGCAAA CCTAGTCATG GCGTCAACGA GAGCCGCTAC 
GGAGGACCCA CTCCTCCCTA CATCCCCAAC CACAAGCTTG TGGTCCCGGA ACCAGTGAAG 
AGCCTGCCTG TGGCCATAGG GGAGCCAAAG GAGGAGGGGC TGGAGGTCCA GATAAGCCGG 
TTGGCAGAGC TGATTGGGAG ACTGGAGAGC AAGACCCTCT CGTTTGACCT GCAGCAGCGC 
CTCTCGGATG AAGAAGGCAC CAACATGTAC CTGCAGCTGG TCCGGCAGGA GATGGCCGTG 
TGCCCTGAGC AGCTGGGCGA GTTCTTGGAT TCCCTGAGAC AGTACCTTCG GAGCACTGCT 
GAAGAAAAGA ACTGCTTCCA TGTGGCCGCG GTGAGGATGG CAGATGGGCT AACCTTCGTC 
ATCTACGAGT TCTGGGAGAC AGAGGAGGAG TGGAAGAAGC ACCTGCAGAG CCCTGTGTGC 
AAGGGCTTCC GACATGTCAA GGTTGACACA CTGAGCCAGC CTGAGGCACT CTCCCAGATC 
TCCGTGCCAG CTGCCTGGTG CACCTCCGGC CGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596906.1
CDS	68..1126
Translation	MGEPRAPLVPDIPAADPDPGPAAPRGGTAVILDIFRRADKNDDGKLSLEEFQLFFADGVLNEKELEGLFHTIDSDNTNHVDTKELCDYFVEHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGNNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQIRQDYCKPSHGVNESRYGGPTPPYIPNHKLVVPEPVKSLPVAIGEPKEEGLEVQISRLAELIGRLESKTLSFDLQQRLSDEEGTNMYLQLVRQEMAVCPEQLGEFLDSLRQYLRSTAEEKNCFHVAAVRMADGLTFVIYEFWETEEEWKKHLQSPVCKGFRHVKVDTLSQPEALSQISVPAAWCTSGRD

Target ORF information:

RefSeq Version	NM_133415.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N-terminal EF-hand calcium binding protein 2 (Necab2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133415.1

ORF Insert Sequence:

ATGGGGGAAC CCCGCGCACC GCTCGTCCCC GACATCCCCG CCGCGGACCC CGACCCCGGG 
CCCGCCGCAC CGCGAGGGGG CACAGCCGTC ATCCTGGACA TTTTCCGCCG TGCGGATAAA 
AACGACGACG GGAAACTGTC CCTTGAGGAA TTTCAACTGT TCTTCGCGGA TGGCGTCCTC 
AATGAGAAGG AGCTGGAGGG CCTCTTCCAC ACCATTGATT CAGACAACAC CAACCATGTG 
GACACAAAGG AATTGTGTGA TTACTTTGTG GAACACATGG GAGACTACGA AGATGTCCTG 
GCCTCCCTCG AGACTTTGAA CCACTCAGTC CTGAAGGCTA TGGGCTACAC CAAGAAGGTG 
TATGAAGGTG GGAACAACGT GGACCAGTTT GTGACCCGCT TCCTTCTGAA GGAGACGGCC 
AATCAGATCC AGTCGCTGCT CAGCTCTGTG GAAAGCGCCG TGGAAGCCAT CGAAGAACAG 
ACCAGCCAGA TCCGACAGGA CTACTGCAAA CCTAGTCATG GCGTCAACGA GAGCCGCTAC 
GGAGGACCCA CTCCTCCCTA CATCCCCAAC CACAAGCTTG TGGTCCCGGA ACCAGTGAAG 
AGCCTGCCTG TGGCCATAGG GGAGCCAAAG GAGGAGGGGC TGGAGGTCCA GATAAGCCGG 
TTGGCAGAGC TGATTGGGAG ACTGGAGAGC AAGACCCTCT CGTTTGACCT GCAGCAGCGC 
CTCTCGGATG AAGAAGGCAC CAACATGTAC CTGCAGCTGG TCCGGCAGGA GATGGCCGTG 
TGCCCTGAGC AGCTGGGCGA GTTCTTGGAT TCCCTGAGAC AGTACCTTCG GAGCACTGCT 
GAAGAAAAGA ACTGCTTCCA TGTGGCCGCG GTGAGGATGG CAGATGGGCT AACCTTCGTC 
ATCTACGAGT TCTGGGAGAC AGAGGAGGAG TGGAAGAAGC ACCTGCAGAG CCCTGTGTGC 
AAGGGCTTCC GACATGTCAA GGTTGACACA CTGAGCCAGC CTGAGGCACT CTCCCAGATC 
TCCGTGCCAG CTGCCTGGTG CACCTCCGGC CGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

The association of metabotropic glutamate receptor type 5 with the neuronal Ca2+-binding protein 2 modulates receptor function.
Journal of neurochemistry111(2)555-67(2009 Oct)
Canela L,Fern?ndez-Due?as V,Albergaria C,Watanabe M,Llu?s C,Mallol J,Canela EI,Franco R,Luj?n R,Ciruela F

The neuronal Ca(2+) -binding protein 2 (NECAB2) interacts with the adenosine A(2A) receptor and modulates the cell surface expression and function of the receptor.
Molecular and cellular neurosciences36(1)1-12(2007 Sep)
Canela L,Luj?n R,Llu?s C,Burgue?o J,Mallol J,Canela EI,Franco R,Ciruela F

NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern.
Neuroscience112(1)51-63(2002)
Sugita S,Ho A,S?dhof TC