Agpat4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Agpat4
Entrez Gene ID	170919
Full Name	1-acylglycerol-3-phosphate O-acyltransferase 4
General protein information	Preferred Names 1-acylglycerol-3-phosphate O-acyltransferase 4 Names 1-acyl-sn-glycerol-3-phosphate acyltransferase delta 1-AGP acyltransferase 4 1-AGPAT 4 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, delta) 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta) LPAAT-delta lysophosphatidic acid acyltransferase delta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q11
Summary	lysophosphatidic acid acyltransferase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006227857.3	XP_006227919.1	1-acyl-sn-glycerol-3-phosphate acyltransferase delta isoform X2
XM_006227856.3	XP_006227918.1	1-acyl-sn-glycerol-3-phosphate acyltransferase delta isoform X1
NM_133406.1	NP_596897.1	1-acyl-sn-glycerol-3-phosphate acyltransferase delta

Pathways from BioSystems

Homologies

Homo sapiens (human)	AGPAT4	NP_064518.1
Pan troglodytes (chimpanzee)	AGPAT4	XP_001153359.2
Gallus gallus (chicken)	AGPAT4	XP_004935617.1
Danio rerio (zebrafish)	agpat4	NP_998157.1
	AGPAT4	XP_001100222.2
Bos taurus (cattle)	AGPAT4	NP_001015537.1
Canis lupus familiaris (dog)	AGPAT4	XP_855143.1
Mus musculus (house mouse)	Agpat4	NP_080920.2
Xenopus tropicalis (tropical clawed frog)	agpat4	NP_001011265.1
Rattus norvegicus (Norway rat)	Agpat4	NP_596897.1

Gene Ontology
Bibliography

Related articles in PubMed

Acylglycerophosphate acyltransferase 4 (AGPAT4) is a mitochondrial lysophosphatidic acid acyltransferase that regulates brain phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol levels.
Bradley RM, Marvyn PM, Aristizabal Henao JJ, Mardian EB, George S, Aucoin MG, Stark KD, Duncan RE
Biochimica et biophysica acta1851(12)1566-76(2015 Dec)

A novel lysophosphatidic acid acyltransferase enzyme (LPAAT4) with a possible role for incorporating docosahexaenoic acid into brain glycerophospholipids.
Eto M, Shindou H, Shimizu T
Biochemical and biophysical research communications443(2)718-24(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Agpat4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Agpat4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa22127	XM_006227857.3 Latest version!	Rattus norvegicus 1-acylglycerol-3-phosphate O-acyltransferase 4 (Agpat4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa00702	NM_133406.1 Latest version!	Rattus norvegicus 1-acylglycerol-3-phosphate O-acyltransferase 4 (Agpat4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa22126	XM_006227856.3 Latest version!	Rattus norvegicus 1-acylglycerol-3-phosphate O-acyltransferase 4 (Agpat4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Agpat4 ( XM_006227857.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006227919.1 Rattus norvegicus 1-acylglycerol-3-phosphate O-acyltransferase 4 (Agpat4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa22127
Clone ID Related Accession (Same CDS sequence)	XM_006227857.3
Accession Version	XM_006227857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-acyl-sn-glycerol-3-phosphate acyltransferase delta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006227857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTCA TCGGGCTGCT GAAGTCCCAG TTTCTGTGTC ACCTGGTCTT CTGCTACGTG 
TTCATCGCCT CCGGGCTCAT TGTCAACGCC ATCCAGCTGT GCACGCTGGT CATCTGGCCC 
ATCAACAAGC AGCTGTTCCG AAAGATAAAC GCCAGACTCT GCTACTGCGT CTCCAGCCAG 
TTGGTGATGC TTCTGGAGTG GTGGTCAGGT ACGGAGTGTA CCATCTACAC CGACCCGAAG 
GCCTCCCCCC ACTACGGGAA GGAAAACGCC ATCGTGGTTC TCAATCACAA GTTTGAGATT 
GACTTTCTCT GTGGCTGGAG CCTGGCTGAG CGCCTAGGGA TCCTGGGGAA CTCCAAAGTC 
CTGGCCAAGA AAGAACTGGC TTATGTCCCC ATCATTGGCT GGATGTGGTA CTTTGTGGAA 
ATGATCTTCT GCACACGCAA GTGGGAGCAA GATCGGCAGA CGGTTGCCAA GAGCCTGCTG 
CACCTCCGGG ACTACCCAGA GAAGTATCTG TTTCTGATCC ACTGTGAGGG CACACGGTTC 
ACAGAGAAGA AACACCAAAT CAGCATGCAG GTAGCCCAAG CCAAGGGGCT GCCCAGCCTC 
AAGCATCACC TGCTGCCACG CACCAAGGGC TTTGCTATCA CTGTGAAGTG CTTGCGAGAT 
GTTGTCCCAG CCGTATATGA CTGTACACTC AATTTCAGAA ACAATGAAAA CCCAACACTG 
CTGGGAGTCT TAAATGGAAA GAAATATCAC GCTGACTGCT ACGTTAGGAG GATCCCCATG 
GAAGACATCC CTGAGGATGA GGACAAGTGC TCAGCCTGGC TACACAAGCT CTACCAGGAG 
AAGGATGCCT TTCAGGAGGA ATACTACAGG ACAGGGGTCT TCCCAGAGAC TCCCTGGGTT 
CCCCCACGGC GGCCCTGGTC TCTGGTCAAC TGGTTGTTCT GGGCGTCGCT GCTGCTCTAT 
CCTTTCTTCC AGTTCCTTGT TAGCATGGTC AGCAGCGGTT CCTCGGTGAC GCTGGCCAGC 
TTGGTCCTCA TCTTCTGCAT GGCCTCCATG GGAGTTCGAT GGATGATTGG TGTGACAGAA 
ATCGACAAGG GCTCTGCCTA TGGCAACATC GACAACAAAC GGAAACAAAC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006227919.1
CDS	567..1703
Translation	MDLIGLLKSQFLCHLVFCYVFIASGLIVNAIQLCTLVIWPINKQLFRKINARLCYCVSSQLVMLLEWWSGTECTIYTDPKASPHYGKENAIVVLNHKFEIDFLCGWSLAERLGILGNSKVLAKKELAYVPIIGWMWYFVEMIFCTRKWEQDRQTVAKSLLHLRDYPEKYLFLIHCEGTRFTEKKHQISMQVAQAKGLPSLKHHLLPRTKGFAITVKCLRDVVPAVYDCTLNFRNNENPTLLGVLNGKKYHADCYVRRIPMEDIPEDEDKCSAWLHKLYQEKDAFQEEYYRTGVFPETPWVPPRRPWSLVNWLFWASLLLYPFFQFLVSMVSSGSSVTLASLVLIFCMASMGVRWMIGVTEIDKGSAYGNIDNKRKQTD

Target ORF information:

RefSeq Version	XM_006227857.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 1-acylglycerol-3-phosphate O-acyltransferase 4 (Agpat4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006227857.3

ORF Insert Sequence:

ATGGACCTCA TCGGGCTGCT GAAGTCCCAG TTTCTGTGTC ACCTGGTCTT CTGCTACGTG 
TTCATCGCCT CCGGGCTCAT TGTCAACGCC ATCCAGCTGT GCACGCTGGT CATCTGGCCC 
ATCAACAAGC AGCTGTTCCG AAAGATAAAC GCCAGACTCT GCTACTGCGT CTCCAGCCAG 
TTGGTGATGC TTCTGGAGTG GTGGTCAGGT ACGGAGTGTA CCATCTACAC CGACCCGAAG 
GCCTCCCCCC ACTACGGGAA GGAAAACGCC ATCGTGGTTC TCAATCACAA GTTTGAGATT 
GACTTTCTCT GTGGCTGGAG CCTGGCTGAG CGCCTAGGGA TCCTGGGGAA CTCCAAAGTC 
CTGGCCAAGA AAGAACTGGC TTATGTCCCC ATCATTGGCT GGATGTGGTA CTTTGTGGAA 
ATGATCTTCT GCACACGCAA GTGGGAGCAA GATCGGCAGA CGGTTGCCAA GAGCCTGCTG 
CACCTCCGGG ACTACCCAGA GAAGTATCTG TTTCTGATCC ACTGTGAGGG CACACGGTTC 
ACAGAGAAGA AACACCAAAT CAGCATGCAG GTAGCCCAAG CCAAGGGGCT GCCCAGCCTC 
AAGCATCACC TGCTGCCACG CACCAAGGGC TTTGCTATCA CTGTGAAGTG CTTGCGAGAT 
GTTGTCCCAG CCGTATATGA CTGTACACTC AATTTCAGAA ACAATGAAAA CCCAACACTG 
CTGGGAGTCT TAAATGGAAA GAAATATCAC GCTGACTGCT ACGTTAGGAG GATCCCCATG 
GAAGACATCC CTGAGGATGA GGACAAGTGC TCAGCCTGGC TACACAAGCT CTACCAGGAG 
AAGGATGCCT TTCAGGAGGA ATACTACAGG ACAGGGGTCT TCCCAGAGAC TCCCTGGGTT 
CCCCCACGGC GGCCCTGGTC TCTGGTCAAC TGGTTGTTCT GGGCGTCGCT GCTGCTCTAT 
CCTTTCTTCC AGTTCCTTGT TAGCATGGTC AGCAGCGGTT CCTCGGTGAC GCTGGCCAGC 
TTGGTCCTCA TCTTCTGCAT GGCCTCCATG GGAGTTCGAT GGATGATTGG TGTGACAGAA 
ATCGACAAGG GCTCTGCCTA TGGCAACATC GACAACAAAC GGAAACAAAC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agpat4 ( NM_133406.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_596897.1 Rattus norvegicus 1-acylglycerol-3-phosphate O-acyltransferase 4 (Agpat4), mRNA.

Price & Availability↑

CloneID	ORa00702
Clone ID Related Accession (Same CDS sequence)	NM_133406.1
Accession Version	NM_133406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	1-acyl-sn-glycerol-3-phosphate acyltransferase delta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB067572.1. ##Evidence-Data-START## Transcript exon combination :: AB067572.1, BC086992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906355 [ECO:0000348] ##Evidence-Data-END##

ATGGACCTCA TCGGGCTGCT GAAGTCCCAG TTTCTGTGTC ACCTGGTCTT CTGCTACGTG 
TTCATCGCCT CCGGGCTCAT TGTCAACGCC ATCCAGCTGT GCACGCTGGT CATCTGGCCC 
ATCAACAAGC AGCTGTTCCG AAAGATAAAC GCCAGACTCT GCTACTGCGT CTCCAGCCAG 
TTGGTGATGC TTCTGGAGTG GTGGTCAGGT ACGGAGTGTA CCATCTACAC CGACCCGAAG 
GCCTCCCCCC ACTACGGGAA GGAAAACGCC ATCGTGGTTC TCAATCACAA GTTTGAGATT 
GACTTTCTCT GTGGCTGGAG CCTGGCTGAG CGCCTAGGGA TCCTGGGGAA CTCCAAAGTC 
CTGGCCAAGA AAGAACTGGC TTATGTCCCC ATCATTGGCT GGATGTGGTA CTTTGTGGAA 
ATGATCTTCT GCACACGCAA GTGGGAGCAA GATCGGCAGA CGGTTGCCAA GAGCCTGCTG 
CACCTCCGGG ACTACCCAGA GAAGTATCTG TTTCTGATCC ACTGTGAGGG CACACGGTTC 
ACAGAGAAGA AACACCAAAT CAGCATGCAG GTGGCCCAAG CCAAGGGGCT GCCCAGCCTC 
AAGCATCACC TGCTGCCACG CACCAAGGGC TTTGCTATCA CTGTGAAGTG CTTGCGAGAT 
GTTGTCCCAG CCGTATATGA CTGTACACTC AATTTCAGAA ACAATGAAAA CCCAACACTG 
CTGGGAGTCT TAAATGGAAA GAAATATCAC GCTGACTGCT ACGTTAGGAG GATCCCCATG 
GAAGACATCC CTGAGGATGA GGACAAGTGC TCAGCCTGGC TACACAAGCT CTACCAGGAG 
AAGGATGCCT TTCAGGAGGA ATACTACAGG ACAGGGGTCT TCCCAGAGAC TCCCTGGGTT 
CCCCCACGGC GGCCCTGGTC TCTGGTCAAC TGGTTGTTCT GGGCGTCGTT GCTGCTCTAT 
CCTTTCTTCC AGTTCCTTGT TAGCATGGTC AGCAGCGGTT CCTCGGTGAC GCTGGCCAGC 
TTGGTCCTCA TCTTCTGCAT GGCCTCCATG GGAGTTCGAT GGATGATTGG TGTGACAGAA 
ATCGACAAGG GCTCTGCCTA TGGCAACATC GACAACAAAC GGAAACAAAC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596897.1
CDS	208..1344
Translation	MDLIGLLKSQFLCHLVFCYVFIASGLIVNAIQLCTLVIWPINKQLFRKINARLCYCVSSQLVMLLEWWSGTECTIYTDPKASPHYGKENAIVVLNHKFEIDFLCGWSLAERLGILGNSKVLAKKELAYVPIIGWMWYFVEMIFCTRKWEQDRQTVAKSLLHLRDYPEKYLFLIHCEGTRFTEKKHQISMQVAQAKGLPSLKHHLLPRTKGFAITVKCLRDVVPAVYDCTLNFRNNENPTLLGVLNGKKYHADCYVRRIPMEDIPEDEDKCSAWLHKLYQEKDAFQEEYYRTGVFPETPWVPPRRPWSLVNWLFWASLLLYPFFQFLVSMVSSGSSVTLASLVLIFCMASMGVRWMIGVTEIDKGSAYGNIDNKRKQTD

Target ORF information:

RefSeq Version	NM_133406.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 1-acylglycerol-3-phosphate O-acyltransferase 4 (Agpat4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133406.1

ORF Insert Sequence:

ATGGACCTCA TCGGGCTGCT GAAGTCCCAG TTTCTGTGTC ACCTGGTCTT CTGCTACGTG 
TTCATCGCCT CCGGGCTCAT TGTCAACGCC ATCCAGCTGT GCACGCTGGT CATCTGGCCC 
ATCAACAAGC AGCTGTTCCG AAAGATAAAC GCCAGACTCT GCTACTGCGT CTCCAGCCAG 
TTGGTGATGC TTCTGGAGTG GTGGTCAGGT ACGGAGTGTA CCATCTACAC CGACCCGAAG 
GCCTCCCCCC ACTACGGGAA GGAAAACGCC ATCGTGGTTC TCAATCACAA GTTTGAGATT 
GACTTTCTCT GTGGCTGGAG CCTGGCTGAG CGCCTAGGGA TCCTGGGGAA CTCCAAAGTC 
CTGGCCAAGA AAGAACTGGC TTATGTCCCC ATCATTGGCT GGATGTGGTA CTTTGTGGAA 
ATGATCTTCT GCACACGCAA GTGGGAGCAA GATCGGCAGA CGGTTGCCAA GAGCCTGCTG 
CACCTCCGGG ACTACCCAGA GAAGTATCTG TTTCTGATCC ACTGTGAGGG CACACGGTTC 
ACAGAGAAGA AACACCAAAT CAGCATGCAG GTGGCCCAAG CCAAGGGGCT GCCCAGCCTC 
AAGCATCACC TGCTGCCACG CACCAAGGGC TTTGCTATCA CTGTGAAGTG CTTGCGAGAT 
GTTGTCCCAG CCGTATATGA CTGTACACTC AATTTCAGAA ACAATGAAAA CCCAACACTG 
CTGGGAGTCT TAAATGGAAA GAAATATCAC GCTGACTGCT ACGTTAGGAG GATCCCCATG 
GAAGACATCC CTGAGGATGA GGACAAGTGC TCAGCCTGGC TACACAAGCT CTACCAGGAG 
AAGGATGCCT TTCAGGAGGA ATACTACAGG ACAGGGGTCT TCCCAGAGAC TCCCTGGGTT 
CCCCCACGGC GGCCCTGGTC TCTGGTCAAC TGGTTGTTCT GGGCGTCGTT GCTGCTCTAT 
CCTTTCTTCC AGTTCCTTGT TAGCATGGTC AGCAGCGGTT CCTCGGTGAC GCTGGCCAGC 
TTGGTCCTCA TCTTCTGCAT GGCCTCCATG GGAGTTCGAT GGATGATTGG TGTGACAGAA 
ATCGACAAGG GCTCTGCCTA TGGCAACATC GACAACAAAC GGAAACAAAC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agpat4 ( XM_006227856.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006227918.1 Rattus norvegicus 1-acylglycerol-3-phosphate O-acyltransferase 4 (Agpat4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22126
Clone ID Related Accession (Same CDS sequence)	XM_006227856.3
Accession Version	XM_006227856.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-acyl-sn-glycerol-3-phosphate acyltransferase delta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006227856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGA TGAACCATAG AGGCAGAGAC GGTTTCCTGA GCCCCGGATC TTTCTCCCTT 
CTGGAACTAT TCCGCCATAC GTACTTGTCT CTTGAGAATC CCCGCATCAT GGACCTCATC 
GGGCTGCTGA AGTCCCAGTT TCTGTGTCAC CTGGTCTTCT GCTACGTGTT CATCGCCTCC 
GGGCTCATTG TCAACGCCAT CCAGCTGTGC ACGCTGGTCA TCTGGCCCAT CAACAAGCAG 
CTGTTCCGAA AGATAAACGC CAGACTCTGC TACTGCGTCT CCAGCCAGTT GGTGATGCTT 
CTGGAGTGGT GGTCAGGTAC GGAGTGTACC ATCTACACCG ACCCGAAGGC CTCCCCCCAC 
TACGGGAAGG AAAACGCCAT CGTGGTTCTC AATCACAAGT TTGAGATTGA CTTTCTCTGT 
GGCTGGAGCC TGGCTGAGCG CCTAGGGATC CTGGGGAACT CCAAAGTCCT GGCCAAGAAA 
GAACTGGCTT ATGTCCCCAT CATTGGCTGG ATGTGGTACT TTGTGGAAAT GATCTTCTGC 
ACACGCAAGT GGGAGCAAGA TCGGCAGACG GTTGCCAAGA GCCTGCTGCA CCTCCGGGAC 
TACCCAGAGA AGTATCTGTT TCTGATCCAC TGTGAGGGCA CACGGTTCAC AGAGAAGAAA 
CACCAAATCA GCATGCAGGT AGCCCAAGCC AAGGGGCTGC CCAGCCTCAA GCATCACCTG 
CTGCCACGCA CCAAGGGCTT TGCTATCACT GTGAAGTGCT TGCGAGATGT TGTCCCAGCC 
GTATATGACT GTACACTCAA TTTCAGAAAC AATGAAAACC CAACACTGCT GGGAGTCTTA 
AATGGAAAGA AATATCACGC TGACTGCTAC GTTAGGAGGA TCCCCATGGA AGACATCCCT 
GAGGATGAGG ACAAGTGCTC AGCCTGGCTA CACAAGCTCT ACCAGGAGAA GGATGCCTTT 
CAGGAGGAAT ACTACAGGAC AGGGGTCTTC CCAGAGACTC CCTGGGTTCC CCCACGGCGG 
CCCTGGTCTC TGGTCAACTG GTTGTTCTGG GCGTCGCTGC TGCTCTATCC TTTCTTCCAG 
TTCCTTGTTA GCATGGTCAG CAGCGGTTCC TCGGTGACGC TGGCCAGCTT GGTCCTCATC 
TTCTGCATGG CCTCCATGGG AGTTCGATGG ATGATTGGTG TGACAGAAAT CGACAAGGGC 
TCTGCCTATG GCAACATCGA CAACAAACGG AAACAAACTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006227918.1
CDS	901..2145
Translation	MAVMNHRGRDGFLSPGSFSLLELFRHTYLSLENPRIMDLIGLLKSQFLCHLVFCYVFIASGLIVNAIQLCTLVIWPINKQLFRKINARLCYCVSSQLVMLLEWWSGTECTIYTDPKASPHYGKENAIVVLNHKFEIDFLCGWSLAERLGILGNSKVLAKKELAYVPIIGWMWYFVEMIFCTRKWEQDRQTVAKSLLHLRDYPEKYLFLIHCEGTRFTEKKHQISMQVAQAKGLPSLKHHLLPRTKGFAITVKCLRDVVPAVYDCTLNFRNNENPTLLGVLNGKKYHADCYVRRIPMEDIPEDEDKCSAWLHKLYQEKDAFQEEYYRTGVFPETPWVPPRRPWSLVNWLFWASLLLYPFFQFLVSMVSSGSSVTLASLVLIFCMASMGVRWMIGVTEIDKGSAYGNIDNKRKQTD

Target ORF information:

RefSeq Version	XM_006227856.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 1-acylglycerol-3-phosphate O-acyltransferase 4 (Agpat4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006227856.3

ORF Insert Sequence:

ATGGCTGTGA TGAACCATAG AGGCAGAGAC GGTTTCCTGA GCCCCGGATC TTTCTCCCTT 
CTGGAACTAT TCCGCCATAC GTACTTGTCT CTTGAGAATC CCCGCATCAT GGACCTCATC 
GGGCTGCTGA AGTCCCAGTT TCTGTGTCAC CTGGTCTTCT GCTACGTGTT CATCGCCTCC 
GGGCTCATTG TCAACGCCAT CCAGCTGTGC ACGCTGGTCA TCTGGCCCAT CAACAAGCAG 
CTGTTCCGAA AGATAAACGC CAGACTCTGC TACTGCGTCT CCAGCCAGTT GGTGATGCTT 
CTGGAGTGGT GGTCAGGTAC GGAGTGTACC ATCTACACCG ACCCGAAGGC CTCCCCCCAC 
TACGGGAAGG AAAACGCCAT CGTGGTTCTC AATCACAAGT TTGAGATTGA CTTTCTCTGT 
GGCTGGAGCC TGGCTGAGCG CCTAGGGATC CTGGGGAACT CCAAAGTCCT GGCCAAGAAA 
GAACTGGCTT ATGTCCCCAT CATTGGCTGG ATGTGGTACT TTGTGGAAAT GATCTTCTGC 
ACACGCAAGT GGGAGCAAGA TCGGCAGACG GTTGCCAAGA GCCTGCTGCA CCTCCGGGAC 
TACCCAGAGA AGTATCTGTT TCTGATCCAC TGTGAGGGCA CACGGTTCAC AGAGAAGAAA 
CACCAAATCA GCATGCAGGT AGCCCAAGCC AAGGGGCTGC CCAGCCTCAA GCATCACCTG 
CTGCCACGCA CCAAGGGCTT TGCTATCACT GTGAAGTGCT TGCGAGATGT TGTCCCAGCC 
GTATATGACT GTACACTCAA TTTCAGAAAC AATGAAAACC CAACACTGCT GGGAGTCTTA 
AATGGAAAGA AATATCACGC TGACTGCTAC GTTAGGAGGA TCCCCATGGA AGACATCCCT 
GAGGATGAGG ACAAGTGCTC AGCCTGGCTA CACAAGCTCT ACCAGGAGAA GGATGCCTTT 
CAGGAGGAAT ACTACAGGAC AGGGGTCTTC CCAGAGACTC CCTGGGTTCC CCCACGGCGG 
CCCTGGTCTC TGGTCAACTG GTTGTTCTGG GCGTCGCTGC TGCTCTATCC TTTCTTCCAG 
TTCCTTGTTA GCATGGTCAG CAGCGGTTCC TCGGTGACGC TGGCCAGCTT GGTCCTCATC 
TTCTGCATGG CCTCCATGGG AGTTCGATGG ATGATTGGTG TGACAGAAAT CGACAAGGGC 
TCTGCCTATG GCAACATCGA CAACAAACGG AAACAAACTG ACTGA