Lfng cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lfng
Entrez Gene ID	170905
Full Name	LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
General protein information	Preferred Names LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase Names beta-1,3-N-acetylglucosaminyltransferase lunatic fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase lunatic fringe gene homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q11
Summary	glycosyltransferase; involved in the epithelial morphogenesis of teeth [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133393.2	NP_596884.1	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe precursor
XM_017598250.1	XP_017453739.1	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LFNG	XP_527647.3
Bos taurus (cattle)	LFNG	NP_001039687.1
Xenopus tropicalis (tropical clawed frog)	lfng	NP_001017051.1
Homo sapiens (human)	LFNG	NP_001035257.1
Mus musculus (house mouse)	Lfng	NP_032520.1
	LOC100428736	XP_002803236.1
Rattus norvegicus (Norway rat)	Lfng	NP_596884.1
Gallus gallus (chicken)	LFNG	NP_990279.1
Canis lupus familiaris (dog)	LFNG	XP_547009.3
Danio rerio (zebrafish)	lfng	NP_571046.1

Gene Ontology
Bibliography

Related articles in PubMed

Deciphering the Fringe-Mediated Notch Code: Identification of Activating and Inhibiting Sites Allowing Discrimination between Ligands.
Kakuda S, Haltiwanger RS
Developmental cell40(2)193-201(2017 01)

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Chiasserini D, van Weering JR, Piersma SR, Pham TV, Malekzadeh A, Teunissen CE, de Wit H, Jim?nez CR
Journal of proteomics106191-204(2014 Jun)

Lfng regulates the synchronized oscillation of the mouse segmentation clock via trans-repression of Notch signalling.
Okubo Y, Sugawara T, Abe-Koduka N, Kanno J, Kimura A, Saga Y
Nature communications31141(2012)

Notch is a critical component of the mouse somitogenesis oscillator and is essential for the formation of the somites.
Ferjentsik Z, Hayashi S, Dale JK, Bessho Y, Herreman A, De Strooper B, del Monte G, de la Pompa JL, Maroto M
PLoS genetics5(9)e1000662(2009 Sep)

Lunatic and manic fringe cooperatively enhance marginal zone B cell precursor competition for delta-like 1 in splenic endothelial niches.
Tan JB, Xu K, Cretegny K, Visan I, Yuan JS, Egan SE, Guidos CJ
Immunity30(2)254-63(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lunatic fringe, FGF, and BMP regulate the Notch pathway during epithelial morphogenesis of teeth.
Title: Lunatic fringe, FGF, and BMP regulate the Notch pathway during epithelial morphogenesis of teeth.

The following Lfng gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lfng cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00668	NM_133393.2 Latest version!	Rattus norvegicus LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Lfng), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa57774	XM_017598250.1 Latest version!	Rattus norvegicus LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Lfng), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lfng ( NM_133393.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_596884.1 Rattus norvegicus LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Lfng), mRNA.

Price & Availability↑

CloneID	ORa00668
Clone ID Related Accession (Same CDS sequence)	NM_133393.2
Accession Version	NM_133393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC070933.1. On May 24, 2004 this sequence version replaced NM_133393.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070933.1, AB054539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGCTCCAGC GCTGCGGCCG GCGCCTACTG CTGGCGCTGG TGGGTGCGCT GCTGGCTTGT 
CTCCTGGTGC TCACCGCCGA CCCGCCGCCA ACTCCGGTGC CCGCTGAGCG CGGAAGGCGC 
GCGCTGCGTA GCCTGGCGGG CTCCTCTGGG GCGGCTCCGG GTCCAGGGTC CAGGGCGGCC 
GTGGATCCCG GAGTCCTCGT CCGCGAGGTG CACAGCCTTT CCGAGTACTT CAGTCTACTC 
ACCCGCGCGC GCAGAGACGC CGATCCACCG CCCGGGGTCG CTTCTCGCCA GGGCGACGGC 
CATCCGCGTC CCCCCGCCGA GGTTCTGTCC CCTCGCGATG TCTTCATCGC CGTCAAGACC 
ACCAGAAAGT TTCACCGCGC GCGGCTTGAC CTGCTGTTCG AGACCTGGAT CTCGCGCCAC 
AAGGAGATGA CATTCATCTT CACGGACGGG GAGGATGAAG CTTTGGCCAA GCACACAGGC 
AATGTGGTAC TCACCAACTG CTCTGCGGCC CACAGCCGCC AGGCGCTGTC TTGCAAGATG 
GCTGTGGAGT ACGACCGTTT CATTGAGTCG GGGAAGAAGT GGTTCTGCCA CGTGGACGAT 
GACAACTACG TCAACCTCCG GGCACTGCTG CGGCTGCTGG CCAGCTATCC CCACACCCAA 
GACGTGTACA TCGGCAAGCC CAGCCTGGAC AGACCCATCC AGGCCACGGA GCGGATCAGC 
GAGCACAGAG TGAGACCTGT CCACTTTTGG TTTGCCACCG GAGGAGCTGG CTTCTGCATT 
AGCCGAGGAC TGGCCCTAAA GATGGGCCCA TGGGCCAGTG GGGGACACTT CATGAGCACA 
GCAGAGCGCA TCCGCCTCCC GGATGATTGC ACCATTGGCT ACATTGTGGA GGCTCTGCTG 
GGCGTGCCCC TCATCCGGAG TGGCCTCTTC CACTCGCACC TGGAGAACCT GCAGCAGGTG 
CCCACCACCG AGCTCCATGA ACAGGTGACC CTGAGCTATG GCATGTTTGA AAACAAGCGG 
AACGCCGTGC ACATCAAGGG ACCCTTCTCA GTGGAGGCTG ACCCTTCCAG GTTCCGCTCT 
ATCCACTGCC ATCTGTACCC GGACACACCC TGGTGTCCCC GCACTGCCAT CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596884.1
CDS	60..1196
Translation	MLQRCGRRLLLALVGALLACLLVLTADPPPTPVPAERGRRALRSLAGSSGAAPGPGSRAAVDPGVLVREVHSLSEYFSLLTRARRDADPPPGVASRQGDGHPRPPAEVLSPRDVFIAVKTTRKFHRARLDLLFETWISRHKEMTFIFTDGEDEALAKHTGNVVLTNCSAAHSRQALSCKMAVEYDRFIESGKKWFCHVDDDNYVNLRALLRLLASYPHTQDVYIGKPSLDRPIQATERISEHRVRPVHFWFATGGAGFCISRGLALKMGPWASGGHFMSTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTTELHEQVTLSYGMFENKRNAVHIKGPFSVEADPSRFRSIHCHLYPDTPWCPRTAIL

Target ORF information:

RefSeq Version	NM_133393.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Lfng), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133393.2

ORF Insert Sequence:

ATGCTCCAGC GCTGCGGCCG GCGCCTACTG CTGGCGCTGG TGGGTGCGCT GCTGGCTTGT 
CTCCTGGTGC TCACCGCCGA CCCGCCGCCA ACTCCGGTGC CCGCTGAGCG CGGAAGGCGC 
GCGCTGCGTA GCCTGGCGGG CTCCTCTGGG GCGGCTCCGG GTCCAGGGTC CAGGGCGGCC 
GTGGATCCCG GAGTCCTCGT CCGCGAGGTG CACAGCCTTT CCGAGTACTT CAGTCTACTC 
ACCCGCGCGC GCAGAGACGC CGATCCACCG CCCGGGGTCG CTTCTCGCCA GGGCGACGGC 
CATCCGCGTC CCCCCGCCGA GGTTCTGTCC CCTCGCGATG TCTTCATCGC CGTCAAGACC 
ACCAGAAAGT TTCACCGCGC GCGGCTTGAC CTGCTGTTCG AGACCTGGAT CTCGCGCCAC 
AAGGAGATGA CATTCATCTT CACGGACGGG GAGGATGAAG CTTTGGCCAA GCACACAGGC 
AATGTGGTAC TCACCAACTG CTCTGCGGCC CACAGCCGCC AGGCGCTGTC TTGCAAGATG 
GCTGTGGAGT ACGACCGTTT CATTGAGTCG GGGAAGAAGT GGTTCTGCCA CGTGGACGAT 
GACAACTACG TCAACCTCCG GGCACTGCTG CGGCTGCTGG CCAGCTATCC CCACACCCAA 
GACGTGTACA TCGGCAAGCC CAGCCTGGAC AGACCCATCC AGGCCACGGA GCGGATCAGC 
GAGCACAGAG TGAGACCTGT CCACTTTTGG TTTGCCACCG GAGGAGCTGG CTTCTGCATT 
AGCCGAGGAC TGGCCCTAAA GATGGGCCCA TGGGCCAGTG GGGGACACTT CATGAGCACA 
GCAGAGCGCA TCCGCCTCCC GGATGATTGC ACCATTGGCT ACATTGTGGA GGCTCTGCTG 
GGCGTGCCCC TCATCCGGAG TGGCCTCTTC CACTCGCACC TGGAGAACCT GCAGCAGGTG 
CCCACCACCG AGCTCCATGA ACAGGTGACC CTGAGCTATG GCATGTTTGA AAACAAGCGG 
AACGCCGTGC ACATCAAGGG ACCCTTCTCA GTGGAGGCTG ACCCTTCCAG GTTCCGCTCT 
ATCCACTGCC ATCTGTACCC GGACACACCC TGGTGTCCCC GCACTGCCAT CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lfng ( XM_017598250.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453739.1 Rattus norvegicus LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Lfng), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57774
Clone ID Related Accession (Same CDS sequence)	XM_017598250.1
Accession Version	XM_017598250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGT GCAGAGGGGG GGGGAGGTGG ATGCGAGTCT GTGCGTGTGG GCTGCCCCGG 
GTGTCTGCAG AGACCTGGAA GGAGCGGTGC ATACTTGAAG ATGATATAGA TCCCACTTTT 
TGCTCTCCTG ACGCGGGCCT CACTGGGACA TTCATCTTCA CGGACGGGGA GGATGAAGCT 
TTGGCCAAGC ACACAGGCAA TGTGGTACTC ACCAACTGCT CTGCGGCCCA CAGCCGCCAG 
GCGCTGTCTT GCAAGATGGC TGTGGAGTAC GACCGTTTCA TTGAGTCGGG GAAGAAGTGG 
TTCTGCCACG TGGACGATGA CAACTACGTC AACCTCCGGG CACTGCTGCG GCTGCTGGCC 
AGCTATCCCC ACACCCAAGA CGTGTACATC GGCAAGCCCA GCCTGGACAG ACCCATCCAG 
GCCACGGAGC GGATCAGCGA GCACAGAGTG AGACCTGTCC ACTTTTGGTT TGCCACCGGA 
GGAGCTGGCT TCTGCATTAG CCGAGGACTG GCCCTAAAGA TGGGCCCATG GGCCAGTGGG 
GGACACTTCA TGAGCACAGC AGAGCGCATC CGCCTCCCGG ATGATTGCAC CATTGGCTAC 
ATTGTGGAGG CTCTGCTGGG CGTGCCCCTC ATCCGGAGTG GCCTCTTCCA CTCGCACCTG 
GAGAACCTGC AGCAGGTGCC CACCACCGAG CTCCATGAAC AGGTGACCCT GAGCTATGGC 
ATGTTTGAAA ACAAGCGGAA CGCCGTGCAC ATCAAGGGAC CCTTCTCAGT GGAGGCTGAC 
CCTTCCAGGT TCCGCTCTAT CCACTGCCAT CTGTACCCGG ACACACCCTG GTGTCCCCGC 
ACTGCCATCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453739.1
CDS	2504..3358
Translation	MLGCRGGGRWMRVCACGLPRVSAETWKERCILEDDIDPTFCSPDAGLTGTFIFTDGEDEALAKHTGNVVLTNCSAAHSRQALSCKMAVEYDRFIESGKKWFCHVDDDNYVNLRALLRLLASYPHTQDVYIGKPSLDRPIQATERISEHRVRPVHFWFATGGAGFCISRGLALKMGPWASGGHFMSTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTTELHEQVTLSYGMFENKRNAVHIKGPFSVEADPSRFRSIHCHLYPDTPWCPRTAIL

Target ORF information:

RefSeq Version	XM_017598250.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Lfng), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598250.1

ORF Insert Sequence:

ATGCTGGGGT GCAGAGGGGG GGGGAGGTGG ATGCGAGTCT GTGCGTGTGG GCTGCCCCGG 
GTGTCTGCAG AGACCTGGAA GGAGCGGTGC ATACTTGAAG ATGATATAGA TCCCACTTTT 
TGCTCTCCTG ACGCGGGCCT CACTGGGACA TTCATCTTCA CGGACGGGGA GGATGAAGCT 
TTGGCCAAGC ACACAGGCAA TGTGGTACTC ACCAACTGCT CTGCGGCCCA CAGCCGCCAG 
GCGCTGTCTT GCAAGATGGC TGTGGAGTAC GACCGTTTCA TTGAGTCGGG GAAGAAGTGG 
TTCTGCCACG TGGACGATGA CAACTACGTC AACCTCCGGG CACTGCTGCG GCTGCTGGCC 
AGCTATCCCC ACACCCAAGA CGTGTACATC GGCAAGCCCA GCCTGGACAG ACCCATCCAG 
GCCACGGAGC GGATCAGCGA GCACAGAGTG AGACCTGTCC ACTTTTGGTT TGCCACCGGA 
GGAGCTGGCT TCTGCATTAG CCGAGGACTG GCCCTAAAGA TGGGCCCATG GGCCAGTGGG 
GGACACTTCA TGAGCACAGC AGAGCGCATC CGCCTCCCGG ATGATTGCAC CATTGGCTAC 
ATTGTGGAGG CTCTGCTGGG CGTGCCCCTC ATCCGGAGTG GCCTCTTCCA CTCGCACCTG 
GAGAACCTGC AGCAGGTGCC CACCACCGAG CTCCATGAAC AGGTGACCCT GAGCTATGGC 
ATGTTTGAAA ACAAGCGGAA CGCCGTGCAC ATCAAGGGAC CCTTCTCAGT GGAGGCTGAC 
CCTTCCAGGT TCCGCTCTAT CCACTGCCAT CTGTACCCGG ACACACCCTG GTGTCCCCGC 
ACTGCCATCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deciphering the Fringe-Mediated Notch Code: Identification of Activating and Inhibiting Sites Allowing Discrimination between Ligands.
Developmental cell40(2)193-201(2017 01)
Kakuda S,Haltiwanger RS

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Journal of proteomics106191-204(2014 Jun)
Chiasserini D,van Weering JR,Piersma SR,Pham TV,Malekzadeh A,Teunissen CE,de Wit H,Jim?nez CR

Lfng regulates the synchronized oscillation of the mouse segmentation clock via trans-repression of Notch signalling.
Nature communications31141(2012)
Okubo Y,Sugawara T,Abe-Koduka N,Kanno J,Kimura A,Saga Y

Notch is a critical component of the mouse somitogenesis oscillator and is essential for the formation of the somites.
PLoS genetics5(9)e1000662(2009 Sep)
Ferjentsik Z,Hayashi S,Dale JK,Bessho Y,Herreman A,De Strooper B,del Monte G,de la Pompa JL,Maroto M

Lunatic and manic fringe cooperatively enhance marginal zone B cell precursor competition for delta-like 1 in splenic endothelial niches.
Immunity30(2)254-63(2009 Feb)
Tan JB,Xu K,Cretegny K,Visan I,Yuan JS,Egan SE,Guidos CJ