Nphs2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nphs2
Entrez Gene ID	170672
Full Name	NPHS2, podocin
Synonyms	podocin
General protein information	Preferred Names NPHS2, podocin Names podocin nephrosis 2 homolog, podocin nephrosis 2, idiopathic, steroid-resistant
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q22
Summary	may play a role in renal glomerular capillary wall function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_130828.2	NP_570841.2	podocin

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NPHS2	XP_005623019.1
Homo sapiens (human)	NPHS2	NP_055440.1
Pan troglodytes (chimpanzee)	NPHS2	XP_003308663.2
Bos taurus (cattle)	NPHS2	NP_001193036.1
Gallus gallus (chicken)	NPHS2	XP_422265.3
Mus musculus (house mouse)	Nphs2	NP_569723.1
Rattus norvegicus (Norway rat)	Nphs2	NP_570841.2
	NPHS2	XP_002802082.1
Danio rerio (zebrafish)	nphs2	NP_001018155.1
Xenopus tropicalis (tropical clawed frog)	nphs2	XP_002937013.2

Gene Ontology
Bibliography

Related articles in PubMed

The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an L-NAME model of pre-eclampsia.
Baijnath S, Murugesan S, Mackraj I, Gathiram P, Moodley J
Molecular and cellular biochemistry427(1-2)59-67(2017 Mar)

Podocin is translocated to cytoplasm in puromycin aminonucleoside nephrosis rats and in poor-prognosis patients with IgA nephropathy.
Fukuda H, Hidaka T, Takagi-Akiba M, Ichimura K, Oliva Trejo JA, Sasaki Y, Wang J, Sakai T, Asanuma K, Tomino Y
Cell and tissue research360(2)391-400(2015 May)

Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin.
Enatsu N, Miyake H, Chiba K, Fujisawa M
BioMed research international2015273784(2015)

Resveratrol increases nephrin and podocin expression and alleviates renal damage in rats fed a high-fat diet.
Pan QR, Ren YL, Zhu JJ, Hu YJ, Zheng JS, Fan H, Xu Y, Wang G, Liu WX
Nutrients6(7)2619-31(2014 Jul)

ATP acting through P2Y receptors causes activation of podocyte TRPC6 channels: role of podocin and reactive oxygen species.
Roshanravan H, Dryer SE
American journal of physiology. Renal physiology306(9)F1088-97(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an L-NAME model of pre-eclampsia.
Title: The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an L-NAME model of pre-eclampsia.

translocation of podocin by endocytosis could be a key traffic event of critical podocyte injury and that the podocin gap could indicate the prognosis of IgA nephropathy.
Title: Podocin is translocated to cytoplasm in puromycin aminonucleoside nephrosis rats and in poor-prognosis patients with IgA nephropathy.

These findings suggest that candesartan can prevent cisplatin-induced testicular damage by regulating expression patterns of the nephrin-podocin complex in the testes.
Title: Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin.

Resveratrol alleviated dyslipidemia and renal damage induced by high-fat diet also reduced TNF-alpha and MCP-1 levels, and reduced expressions of nephrin and podocin.
Title: Resveratrol increases nephrin and podocin expression and alleviates renal damage in rats fed a high-fat diet.

ATP evokes activation of TRPC6 channels in podocytes through P2Y receptors, podocin, and generation of reactive oxygen species.
Title: ATP acting through P2Y receptors causes activation of podocyte TRPC6 channels: role of podocin and reactive oxygen species.

The following Nphs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nphs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00496	NM_130828.2 Latest version!	Rattus norvegicus NPHS2 stomatin family member, podocin (Nphs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nphs2 ( NM_130828.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_570841.2 Rattus norvegicus NPHS2 stomatin family member, podocin (Nphs2), mRNA.

Price & Availability↑

CloneID	ORa00496
Clone ID Related Accession (Same CDS sequence)	NM_130828.2
Accession Version	NM_130828.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	podocin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY039651.1. On Jun 9, 2003 this sequence version replaced NM_130828.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY039651.1, BC081687.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642745 [ECO:0000348] ##Evidence-Data-END##

ATGGACAGCA GGGCGCGGAG CTCTTCCAGA AAGACCCACG GGAGAGGTAG CAGGTCCTCT 
TCTAGGGATG ACAAGAAGTC AAAGGCCGGG AGGGGCAACA GAGGCCGCGC GCGCCCGGAT 
GCTGGGGTAG AGCGGCAGAG CGCCGGGCGG ATGGGGACCC GGGAGGAGCA CCGAGCTCCA 
GCAGCCACGG TAGTGAATGT GGACGAGGTT CGAAGCCCGG GTGAGGAGGG TCGGAAAGTG 
GTGGCCCTGC TGGAGAGCGA GCGACCAGAG GAAGGGATCA AGCCCTCTGG ATTAGGGGCC 
TGCGAGTGGC TTCTTGTCCT CTCCTCCCTG ATCTTCATCA TCGTAACGTT TCCCTTTTCC 
ATCTGGTTCT GCATAAAGGT TGTTCAAGAA TACGAAAGAG TAATTATATT CCGACTGGGA 
CATCTGCTTC CTGGAAGAGC CAAAGGACCT GGCCTGTTCT TTTTTCTACC CTGCCTGGAC 
ACCTATCACA AGGTTGACCT CCGTCTCCAG ACCTTGGAAA TACCTTTCCA TGAGGTGGTA 
ACCAAAGATA TGTTCATAAT GGAGATAGAC GCTGTCTGCT ACTACCGCAT GGAAAATGCC 
TCCCTTCTTC TAAGCAGTCT AGCTCATGTG TCCAAAGCCA TCCAGTTCCT GGTGCAAACC 
ACCATGAAGC GCCTCTTGGC ACATCGATCC CTCACTGAAA TTCTCCTGGA AAGGAAGAGC 
ATTGCCCAAG ATGTAAAGGT TGCCTTGGAC TCAGTGACCT GTGTTTGGGG CATCAAAGTG 
GAGAGAACTG AAATTAAGGA TGTACGGCTG CCAGCTGGGC TTCAGCACTC TCTGGCTGTG 
GAAGCTGAGG CACAAAGACA GGCCAAAGTG CGGGTGATTG CTGCCGAAGG GGAAAAAGCT 
GCCTCTGAGT CCCTGAGGAT GGCGGCTGAG ATTCTGTCAG GCACCCCAGC TGCTGTCCAG 
CTCCGGTACC TGCACACTCT TCAGTCCTTG TCCACAGACA AGCCGTCCAC CGTGGTTTTG 
CCTTTACCCT TTGACATGCT GAACCTTTTC TCCTCTCCCA GCAACAGAGC ACAAGGAAGC 
ATCAACTACC CAAGTTCTCC CAAACCTGTT GAACCACTAA ATCCCAAAAG GAAGGACTCT 
CCTATGCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570841.2
CDS	62..1213
Translation	MDSRARSSSRKTHGRGSRSSSRDDKKSKAGRGNRGRARPDAGVERQSAGRMGTREEHRAPAATVVNVDEVRSPGEEGRKVVALLESERPEEGIKPSGLGACEWLLVLSSLIFIIVTFPFSIWFCIKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLDTYHKVDLRLQTLEIPFHEVVTKDMFIMEIDAVCYYRMENASLLLSSLAHVSKAIQFLVQTTMKRLLAHRSLTEILLERKSIAQDVKVALDSVTCVWGIKVERTEIKDVRLPAGLQHSLAVEAEAQRQAKVRVIAAEGEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTDKPSTVVLPLPFDMLNLFSSPSNRAQGSINYPSSPKPVEPLNPKRKDSPML

Target ORF information:

RefSeq Version	NM_130828.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NPHS2 stomatin family member, podocin (Nphs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130828.2

ORF Insert Sequence:

ATGGACAGCA GGGCGCGGAG CTCTTCCAGA AAGACCCACG GGAGAGGTAG CAGGTCCTCT 
TCTAGGGATG ACAAGAAGTC AAAGGCCGGG AGGGGCAACA GAGGCCGCGC GCGCCCGGAT 
GCTGGGGTAG AGCGGCAGAG CGCCGGGCGG ATGGGGACCC GGGAGGAGCA CCGAGCTCCA 
GCAGCCACGG TAGTGAATGT GGACGAGGTT CGAAGCCCGG GTGAGGAGGG TCGGAAAGTG 
GTGGCCCTGC TGGAGAGCGA GCGACCAGAG GAAGGGATCA AGCCCTCTGG ATTAGGGGCC 
TGCGAGTGGC TTCTTGTCCT CTCCTCCCTG ATCTTCATCA TCGTAACGTT TCCCTTTTCC 
ATCTGGTTCT GCATAAAGGT TGTTCAAGAA TACGAAAGAG TAATTATATT CCGACTGGGA 
CATCTGCTTC CTGGAAGAGC CAAAGGACCT GGCCTGTTCT TTTTTCTACC CTGCCTGGAC 
ACCTATCACA AGGTTGACCT CCGTCTCCAG ACCTTGGAAA TACCTTTCCA TGAGGTGGTA 
ACCAAAGATA TGTTCATAAT GGAGATAGAC GCTGTCTGCT ACTACCGCAT GGAAAATGCC 
TCCCTTCTTC TAAGCAGTCT AGCTCATGTG TCCAAAGCCA TCCAGTTCCT GGTGCAAACC 
ACCATGAAGC GCCTCTTGGC ACATCGATCC CTCACTGAAA TTCTCCTGGA AAGGAAGAGC 
ATTGCCCAAG ATGTAAAGGT TGCCTTGGAC TCAGTGACCT GTGTTTGGGG CATCAAAGTG 
GAGAGAACTG AAATTAAGGA TGTACGGCTG CCAGCTGGGC TTCAGCACTC TCTGGCTGTG 
GAAGCTGAGG CACAAAGACA GGCCAAAGTG CGGGTGATTG CTGCCGAAGG GGAAAAAGCT 
GCCTCTGAGT CCCTGAGGAT GGCGGCTGAG ATTCTGTCAG GCACCCCAGC TGCTGTCCAG 
CTCCGGTACC TGCACACTCT TCAGTCCTTG TCCACAGACA AGCCGTCCAC CGTGGTTTTG 
CCTTTACCCT TTGACATGCT GAACCTTTTC TCCTCTCCCA GCAACAGAGC ACAAGGAAGC 
ATCAACTACC CAAGTTCTCC CAAACCTGTT GAACCACTAA ATCCCAAAAG GAAGGACTCT 
CCTATGCTAT AG