Pex12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pex12
Entrez Gene ID	116718
Full Name	peroxisomal biogenesis factor 12
General protein information	Preferred Names peroxisomal biogenesis factor 12 Names peroxisome assembly protein 12 PAF-3 peroxin-12 peroxisome assembly factor 3 peroxisome assembly protein 12-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26
Summary	required for peroxisome biogenesis; functionally complements the mutation in fibroblasts from patients with Zellweger syndrome of complementation group III [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596963.1	XP_017452452.1	peroxisome assembly protein 12 isoform X1
XM_008767934.2	XP_008766156.1	peroxisome assembly protein 12 isoform X1
NM_053921.1	NP_446373.1	peroxisome assembly protein 12

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PEX12	NP_001076847.1
Schizosaccharomyces pombe (fission yeast)	SPAPB17E12.03	NP_001018219.2
Pan troglodytes (chimpanzee)	PEX12	XP_523610.2
Danio rerio (zebrafish)	pex12	NP_956499.1
Drosophila melanogaster (fruit fly)	Pex12	NP_608546.1
Mus musculus (house mouse)	Pex12	NP_598786.1
Rattus norvegicus (Norway rat)	Pex12	NP_446373.1
Magnaporthe oryzae (rice blast fungus)	MGG_01771	XP_003714773.1
Neurospora crassa	NCU05245	XP_962009.1
Oryza sativa (rice)	Os10g0467200	NP_001064809.1
Xenopus tropicalis (tropical clawed frog)	pex12	XP_004911685.1
Canis lupus familiaris (dog)	PEX12	XP_548259.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010497	XP_001237561.2
Arabidopsis thaliana (thale cress)	PEX12	NP_187096.2
Homo sapiens (human)	PEX12	NP_000277.1
Rattus norvegicus (Norway rat)	LOC100909787	XP_006247088.1
Gallus gallus (chicken)	PEX12	XP_415773.2
Caenorhabditis elegans (roundworm)	prx-12	NP_509908.1

Gene Ontology
Bibliography

Related articles in PubMed

PEX14 is required for microtubule-based peroxisome motility in human cells.
Bharti P, Schliebs W, Schievelbusch T, Neuhaus A, David C, Kock K, Herrmann C, Meyer HE, Wiese S, Warscheid B, Theiss C, Erdmann R
Journal of cell science124(Pt 10)1759-68(2011 May)

A novel PEX12 mutation identified as the cause of a peroxisomal biogenesis disorder with mild clinical phenotype, mild biochemical abnormalities in fibroblasts and a mosaic catalase immunofluorescence pattern, even at 40 degrees C.
Zeharia A, Ebberink MS, Wanders RJ, Waterham HR, Gutman A, Nissenkorn A, Korman SH
Journal of human genetics52(7)599-606(2007)

The Arabidopsis pex12 and pex13 mutants are defective in both PTS1- and PTS2-dependent protein transport to peroxisomes.
Mano S, Nakamori C, Nito K, Kondo M, Nishimura M
The Plant journal : for cell and molecular biology47(4)604-18(2006 Aug)

Proteomic analysis of rat liver peroxisome: presence of peroxisome-specific isozyme of Lon protease.
Kikuchi M, Hatano N, Yokota S, Shimozawa N, Imanaka T, Taniguchi H
The Journal of biological chemistry279(1)421-8(2004 Jan)

Characterization of the mammalian peroxisomal import machinery: Pex2p, Pex5p, Pex12p, and Pex14p are subunits of the same protein assembly.
Reguenga C, Oliveira ME, Gouveia AM, S?-Miranda C, Azevedo JE
The Journal of biological chemistry276(32)29935-42(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pex12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pex12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11063	NM_053921.1 Latest version!	Rattus norvegicus peroxisomal biogenesis factor 12 (Pex12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa11063	XM_008767934.2 Latest version!	Rattus norvegicus peroxisomal biogenesis factor 12 (Pex12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa11063	XM_017596963.1 Latest version!	Rattus norvegicus peroxisomal biogenesis factor 12 (Pex12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pex12 ( NM_053921.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446373.1 Rattus norvegicus peroxisomal biogenesis factor 12 (Pex12), mRNA.

Price & Availability↑

CloneID	ORa11063
Clone ID Related Accession (Same CDS sequence)	XM_017596963.1 , XM_008767934.2 , NM_053921.1
Accession Version	NM_053921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	peroxisome assembly protein 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB002111.1. ##Evidence-Data-START## Transcript exon combination :: AB002111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGAGC ATGGGGCTCA TATCACAACT GCTTCGGTGG CGGACGACCA GCCATCCATC 
TTTGAGGTGG TAGCACAGGA CAGTTTAATG ACAGCAGTGA GACCCGCTCT TCAGCATGTG 
GTCAAGGTTC TTGCAGAATC AAATCCTGCG CACTATGGCT TCTTTTGGAG GTGGTTTGAT 
GAAATCTTCA CTCTGCTAGA CTTTCTGCTC CAGCAGCATT ATCTTTCTAG AACTAGTGCC 
TCATTTTCTG AACACTTTTA TGGGTTAAAG CGGATTGTAG CGGGGAGCTC GCCGCAGCTT 
CAGAGACCAG CCAGTGCTGG CCTCCCCAAG GAACACCTCT GGAAATCCGC TATGTTCCTT 
GTTCTTCTTC CCTATCTGAA AGTGAAGCTG GAGAAGCTGG CTTCTACCTT GAGAGAAGAA 
GACGAATACT CTATTCACCC CCCTTCTTCT CACTGGAAAC GATTCTACAG AGTCTTCCTG 
GCTGCCTACC CGTTTGTTAC CATGACCTGG GAAGGCTGGT TCCTTACACA ACAACTTCGG 
TACATATTGG GAAAAGCTGA GCATCACTCT CCCCTGCTGA AACTGGCTGG AGTTCGGCTA 
GGTCGACTGA CAGCTCAGGA CATACAAGCT ATGGAACACA GACTGGTTGA AGCCAGTGCA 
ATGCAGGAGC CCGTCAGAAG CATTGGTAAG AAGATAAAGT CAGCACTGAA GAAAGCTGTG 
GGAGGTGTTG CCTTATCCCT CTCTACTGGC CTCTCTGTGG GTGTATTCTT CCTGCAGTTT 
CTTGACTGGT GGTACTCTTC TGAGAACCAA GAGACCATCA AGTCACTGAC TGCCCTGCCC 
ACTCCACCAC CACCTGTGCA CCTCGACTAC AACTCTGATT CTCCCCTCTT ACCCAAAATG 
AAAACAGTGT GCCCACTGTG TCGTAAAGCT CGGGTCAATG ATACGGTTCT TGCCACCTCT 
GGCTATGTAT TCTGTTACCG CTGTGTGTTT AATTATGTGA GAAGTCACCA AGCCTGTCCC 
ATTACAGGGT ATCCAACGGA AGTCCAGCAT CTAATTAAAC TCTACTCTCC AGAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446373.1
CDS	322..1401
Translation	MAEHGAHITTASVADDQPSIFEVVAQDSLMTAVRPALQHVVKVLAESNPAHYGFFWRWFDEIFTLLDFLLQQHYLSRTSASFSEHFYGLKRIVAGSSPQLQRPASAGLPKEHLWKSAMFLVLLPYLKVKLEKLASTLREEDEYSIHPPSSHWKRFYRVFLAAYPFVTMTWEGWFLTQQLRYILGKAEHHSPLLKLAGVRLGRLTAQDIQAMEHRLVEASAMQEPVRSIGKKIKSALKKAVGGVALSLSTGLSVGVFFLQFLDWWYSSENQETIKSLTALPTPPPPVHLDYNSDSPLLPKMKTVCPLCRKARVNDTVLATSGYVFCYRCVFNYVRSHQACPITGYPTEVQHLIKLYSPEN

Target ORF information:

RefSeq Version	NM_053921.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus peroxisomal biogenesis factor 12 (Pex12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053921.1

ORF Insert Sequence:

ATGGCTGAGC ATGGGGCTCA TATCACAACT GCTTCGGTGG CGGACGACCA GCCATCCATC 
TTTGAGGTGG TAGCACAGGA CAGTTTAATG ACAGCAGTGA GACCCGCTCT TCAGCATGTG 
GTCAAGGTTC TTGCAGAATC AAATCCTGCG CACTATGGCT TCTTTTGGAG GTGGTTTGAT 
GAAATCTTCA CTCTGCTAGA CTTTCTGCTC CAGCAGCATT ATCTTTCTAG AACTAGTGCC 
TCATTTTCTG AACACTTTTA TGGGTTAAAG CGGATTGTAG CGGGGAGCTC GCCGCAGCTT 
CAGAGACCAG CCAGTGCTGG CCTCCCCAAG GAACACCTCT GGAAATCCGC TATGTTCCTT 
GTTCTTCTTC CCTATCTGAA AGTGAAGCTG GAGAAGCTGG CTTCTACCTT GAGAGAAGAA 
GACGAATACT CTATTCACCC CCCTTCTTCT CACTGGAAAC GATTCTACAG AGTCTTCCTG 
GCTGCCTACC CGTTTGTTAC CATGACCTGG GAAGGCTGGT TCCTTACACA ACAACTTCGG 
TACATATTGG GAAAAGCTGA GCATCACTCT CCCCTGCTGA AACTGGCTGG AGTTCGGCTA 
GGTCGACTGA CAGCTCAGGA CATACAAGCT ATGGAACACA GACTGGTTGA AGCCAGTGCA 
ATGCAGGAGC CCGTCAGAAG CATTGGTAAG AAGATAAAGT CAGCACTGAA GAAAGCTGTG 
GGAGGTGTTG CCTTATCCCT CTCTACTGGC CTCTCTGTGG GTGTATTCTT CCTGCAGTTT 
CTTGACTGGT GGTACTCTTC TGAGAACCAA GAGACCATCA AGTCACTGAC TGCCCTGCCC 
ACTCCACCAC CACCTGTGCA CCTCGACTAC AACTCTGATT CTCCCCTCTT ACCCAAAATG 
AAAACAGTGT GCCCACTGTG TCGTAAAGCT CGGGTCAATG ATACGGTTCT TGCCACCTCT 
GGCTATGTAT TCTGTTACCG CTGTGTGTTT AATTATGTGA GAAGTCACCA AGCCTGTCCC 
ATTACAGGGT ATCCAACGGA AGTCCAGCAT CTAATTAAAC TCTACTCTCC AGAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pex12 ( XM_017596963.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452452.1 Rattus norvegicus peroxisomal biogenesis factor 12 (Pex12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa11063
Clone ID Related Accession (Same CDS sequence)	XM_017596963.1 , XM_008767934.2 , NM_053921.1
Accession Version	XM_017596963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	peroxisome assembly protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGC ATGGGGCTCA TATCACAACT GCTTCGGTGG CGGACGACCA GCCATCCATC 
TTTGAGGTGG TAGCACAGGA CAGTTTAATG ACAGCAGTGA GACCCGCTCT TCAGCATGTG 
GTCAAGGTTC TTGCAGAATC AAATCCTGCG CACTATGGCT TCTTTTGGAG GTGGTTTGAT 
GAAATCTTCA CTCTGCTAGA CTTTCTGCTC CAGCAGCATT ATCTTTCTAG AACTAGTGCC 
TCATTTTCTG AACACTTTTA TGGGTTAAAG CGGATTGTAG CGGGGAGCTC GCCGCAGCTT 
CAGAGACCAG CCAGTGCTGG CCTCCCCAAG GAACACCTCT GGAAATCCGC TATGTTCCTT 
GTTCTTCTTC CCTATCTGAA AGTGAAGCTG GAGAAGCTGG CTTCTACCTT GAGAGAAGAA 
GACGAATACT CTATTCACCC CCCTTCTTCT CACTGGAAAC GATTCTACAG AGTCTTCCTG 
GCTGCCTACC CGTTTGTTAC CATGACCTGG GAAGGCTGGT TCCTTACACA ACAACTTCGG 
TACATATTGG GAAAAGCTGA GCATCACTCT CCCCTGCTGA AACTGGCTGG AGTTCGGCTA 
GGTCGACTGA CAGCTCAGGA CATACAAGCT ATGGAACACA GACTGGTTGA AGCCAGTGCA 
ATGCAGGAGC CCGTCAGAAG CATTGGTAAG AAGATAAAGT CAGCACTGAA GAAAGCTGTG 
GGAGGTGTTG CCTTATCCCT CTCTACTGGC CTCTCTGTGG GTGTATTCTT CCTGCAGTTT 
CTTGACTGGT GGTACTCTTC TGAGAACCAA GAGACCATCA AGTCACTGAC TGCCCTGCCC 
ACTCCACCAC CACCTGTGCA CCTCGACTAC AACTCTGATT CTCCCCTCTT ACCCAAAATG 
AAAACAGTGT GCCCACTGTG TCGTAAAGCT CGGGTCAATG ATACGGTTCT TGCCACCTCT 
GGCTATGTAT TCTGTTACCG CTGTGTGTTT AATTATGTGA GAAGTCACCA AGCCTGTCCC 
ATTACAGGGT ATCCAACGGA AGTCCAGCAT CTAATTAAAC TCTACTCTCC AGAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452452.1
CDS	198..1277
Translation	MAEHGAHITTASVADDQPSIFEVVAQDSLMTAVRPALQHVVKVLAESNPAHYGFFWRWFDEIFTLLDFLLQQHYLSRTSASFSEHFYGLKRIVAGSSPQLQRPASAGLPKEHLWKSAMFLVLLPYLKVKLEKLASTLREEDEYSIHPPSSHWKRFYRVFLAAYPFVTMTWEGWFLTQQLRYILGKAEHHSPLLKLAGVRLGRLTAQDIQAMEHRLVEASAMQEPVRSIGKKIKSALKKAVGGVALSLSTGLSVGVFFLQFLDWWYSSENQETIKSLTALPTPPPPVHLDYNSDSPLLPKMKTVCPLCRKARVNDTVLATSGYVFCYRCVFNYVRSHQACPITGYPTEVQHLIKLYSPEN

Target ORF information:

RefSeq Version	XM_017596963.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus peroxisomal biogenesis factor 12 (Pex12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596963.1

ORF Insert Sequence:

ATGGCTGAGC ATGGGGCTCA TATCACAACT GCTTCGGTGG CGGACGACCA GCCATCCATC 
TTTGAGGTGG TAGCACAGGA CAGTTTAATG ACAGCAGTGA GACCCGCTCT TCAGCATGTG 
GTCAAGGTTC TTGCAGAATC AAATCCTGCG CACTATGGCT TCTTTTGGAG GTGGTTTGAT 
GAAATCTTCA CTCTGCTAGA CTTTCTGCTC CAGCAGCATT ATCTTTCTAG AACTAGTGCC 
TCATTTTCTG AACACTTTTA TGGGTTAAAG CGGATTGTAG CGGGGAGCTC GCCGCAGCTT 
CAGAGACCAG CCAGTGCTGG CCTCCCCAAG GAACACCTCT GGAAATCCGC TATGTTCCTT 
GTTCTTCTTC CCTATCTGAA AGTGAAGCTG GAGAAGCTGG CTTCTACCTT GAGAGAAGAA 
GACGAATACT CTATTCACCC CCCTTCTTCT CACTGGAAAC GATTCTACAG AGTCTTCCTG 
GCTGCCTACC CGTTTGTTAC CATGACCTGG GAAGGCTGGT TCCTTACACA ACAACTTCGG 
TACATATTGG GAAAAGCTGA GCATCACTCT CCCCTGCTGA AACTGGCTGG AGTTCGGCTA 
GGTCGACTGA CAGCTCAGGA CATACAAGCT ATGGAACACA GACTGGTTGA AGCCAGTGCA 
ATGCAGGAGC CCGTCAGAAG CATTGGTAAG AAGATAAAGT CAGCACTGAA GAAAGCTGTG 
GGAGGTGTTG CCTTATCCCT CTCTACTGGC CTCTCTGTGG GTGTATTCTT CCTGCAGTTT 
CTTGACTGGT GGTACTCTTC TGAGAACCAA GAGACCATCA AGTCACTGAC TGCCCTGCCC 
ACTCCACCAC CACCTGTGCA CCTCGACTAC AACTCTGATT CTCCCCTCTT ACCCAAAATG 
AAAACAGTGT GCCCACTGTG TCGTAAAGCT CGGGTCAATG ATACGGTTCT TGCCACCTCT 
GGCTATGTAT TCTGTTACCG CTGTGTGTTT AATTATGTGA GAAGTCACCA AGCCTGTCCC 
ATTACAGGGT ATCCAACGGA AGTCCAGCAT CTAATTAAAC TCTACTCTCC AGAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pex12 ( XM_008767934.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766156.1 Rattus norvegicus peroxisomal biogenesis factor 12 (Pex12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa11063
Clone ID Related Accession (Same CDS sequence)	XM_017596963.1 , XM_008767934.2 , NM_053921.1
Accession Version	XM_008767934.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	peroxisome assembly protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGC ATGGGGCTCA TATCACAACT GCTTCGGTGG CGGACGACCA GCCATCCATC 
TTTGAGGTGG TAGCACAGGA CAGTTTAATG ACAGCAGTGA GACCCGCTCT TCAGCATGTG 
GTCAAGGTTC TTGCAGAATC AAATCCTGCG CACTATGGCT TCTTTTGGAG GTGGTTTGAT 
GAAATCTTCA CTCTGCTAGA CTTTCTGCTC CAGCAGCATT ATCTTTCTAG AACTAGTGCC 
TCATTTTCTG AACACTTTTA TGGGTTAAAG CGGATTGTAG CGGGGAGCTC GCCGCAGCTT 
CAGAGACCAG CCAGTGCTGG CCTCCCCAAG GAACACCTCT GGAAATCCGC TATGTTCCTT 
GTTCTTCTTC CCTATCTGAA AGTGAAGCTG GAGAAGCTGG CTTCTACCTT GAGAGAAGAA 
GACGAATACT CTATTCACCC CCCTTCTTCT CACTGGAAAC GATTCTACAG AGTCTTCCTG 
GCTGCCTACC CGTTTGTTAC CATGACCTGG GAAGGCTGGT TCCTTACACA ACAACTTCGG 
TACATATTGG GAAAAGCTGA GCATCACTCT CCCCTGCTGA AACTGGCTGG AGTTCGGCTA 
GGTCGACTGA CAGCTCAGGA CATACAAGCT ATGGAACACA GACTGGTTGA AGCCAGTGCA 
ATGCAGGAGC CCGTCAGAAG CATTGGTAAG AAGATAAAGT CAGCACTGAA GAAAGCTGTG 
GGAGGTGTTG CCTTATCCCT CTCTACTGGC CTCTCTGTGG GTGTATTCTT CCTGCAGTTT 
CTTGACTGGT GGTACTCTTC TGAGAACCAA GAGACCATCA AGTCACTGAC TGCCCTGCCC 
ACTCCACCAC CACCTGTGCA CCTCGACTAC AACTCTGATT CTCCCCTCTT ACCCAAAATG 
AAAACAGTGT GCCCACTGTG TCGTAAAGCT CGGGTCAATG ATACGGTTCT TGCCACCTCT 
GGCTATGTAT TCTGTTACCG CTGTGTGTTT AATTATGTGA GAAGTCACCA AGCCTGTCCC 
ATTACAGGGT ATCCAACGGA AGTCCAGCAT CTAATTAAAC TCTACTCTCC AGAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766156.1
CDS	196..1275
Translation	MAEHGAHITTASVADDQPSIFEVVAQDSLMTAVRPALQHVVKVLAESNPAHYGFFWRWFDEIFTLLDFLLQQHYLSRTSASFSEHFYGLKRIVAGSSPQLQRPASAGLPKEHLWKSAMFLVLLPYLKVKLEKLASTLREEDEYSIHPPSSHWKRFYRVFLAAYPFVTMTWEGWFLTQQLRYILGKAEHHSPLLKLAGVRLGRLTAQDIQAMEHRLVEASAMQEPVRSIGKKIKSALKKAVGGVALSLSTGLSVGVFFLQFLDWWYSSENQETIKSLTALPTPPPPVHLDYNSDSPLLPKMKTVCPLCRKARVNDTVLATSGYVFCYRCVFNYVRSHQACPITGYPTEVQHLIKLYSPEN

Target ORF information:

RefSeq Version	XM_008767934.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus peroxisomal biogenesis factor 12 (Pex12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767934.2

ORF Insert Sequence:

ATGGCTGAGC ATGGGGCTCA TATCACAACT GCTTCGGTGG CGGACGACCA GCCATCCATC 
TTTGAGGTGG TAGCACAGGA CAGTTTAATG ACAGCAGTGA GACCCGCTCT TCAGCATGTG 
GTCAAGGTTC TTGCAGAATC AAATCCTGCG CACTATGGCT TCTTTTGGAG GTGGTTTGAT 
GAAATCTTCA CTCTGCTAGA CTTTCTGCTC CAGCAGCATT ATCTTTCTAG AACTAGTGCC 
TCATTTTCTG AACACTTTTA TGGGTTAAAG CGGATTGTAG CGGGGAGCTC GCCGCAGCTT 
CAGAGACCAG CCAGTGCTGG CCTCCCCAAG GAACACCTCT GGAAATCCGC TATGTTCCTT 
GTTCTTCTTC CCTATCTGAA AGTGAAGCTG GAGAAGCTGG CTTCTACCTT GAGAGAAGAA 
GACGAATACT CTATTCACCC CCCTTCTTCT CACTGGAAAC GATTCTACAG AGTCTTCCTG 
GCTGCCTACC CGTTTGTTAC CATGACCTGG GAAGGCTGGT TCCTTACACA ACAACTTCGG 
TACATATTGG GAAAAGCTGA GCATCACTCT CCCCTGCTGA AACTGGCTGG AGTTCGGCTA 
GGTCGACTGA CAGCTCAGGA CATACAAGCT ATGGAACACA GACTGGTTGA AGCCAGTGCA 
ATGCAGGAGC CCGTCAGAAG CATTGGTAAG AAGATAAAGT CAGCACTGAA GAAAGCTGTG 
GGAGGTGTTG CCTTATCCCT CTCTACTGGC CTCTCTGTGG GTGTATTCTT CCTGCAGTTT 
CTTGACTGGT GGTACTCTTC TGAGAACCAA GAGACCATCA AGTCACTGAC TGCCCTGCCC 
ACTCCACCAC CACCTGTGCA CCTCGACTAC AACTCTGATT CTCCCCTCTT ACCCAAAATG 
AAAACAGTGT GCCCACTGTG TCGTAAAGCT CGGGTCAATG ATACGGTTCT TGCCACCTCT 
GGCTATGTAT TCTGTTACCG CTGTGTGTTT AATTATGTGA GAAGTCACCA AGCCTGTCCC 
ATTACAGGGT ATCCAACGGA AGTCCAGCAT CTAATTAAAC TCTACTCTCC AGAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PEX14 is required for microtubule-based peroxisome motility in human cells.
Journal of cell science124(Pt 10)1759-68(2011 May)
Bharti P,Schliebs W,Schievelbusch T,Neuhaus A,David C,Kock K,Herrmann C,Meyer HE,Wiese S,Warscheid B,Theiss C,Erdmann R

A novel PEX12 mutation identified as the cause of a peroxisomal biogenesis disorder with mild clinical phenotype, mild biochemical abnormalities in fibroblasts and a mosaic catalase immunofluorescence pattern, even at 40 degrees C.
Journal of human genetics52(7)599-606(2007)
Zeharia A,Ebberink MS,Wanders RJ,Waterham HR,Gutman A,Nissenkorn A,Korman SH

The Arabidopsis pex12 and pex13 mutants are defective in both PTS1- and PTS2-dependent protein transport to peroxisomes.
The Plant journal : for cell and molecular biology47(4)604-18(2006 Aug)
Mano S,Nakamori C,Nito K,Kondo M,Nishimura M

Proteomic analysis of rat liver peroxisome: presence of peroxisome-specific isozyme of Lon protease.
The Journal of biological chemistry279(1)421-8(2004 Jan)
Kikuchi M,Hatano N,Yokota S,Shimozawa N,Imanaka T,Taniguchi H

Characterization of the mammalian peroxisomal import machinery: Pex2p, Pex5p, Pex12p, and Pex14p are subunits of the same protein assembly.
The Journal of biological chemistry276(32)29935-42(2001 Aug)
Reguenga C,Oliveira ME,Gouveia AM,S?-Miranda C,Azevedo JE