Dnase1l3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnase1l3
Entrez Gene ID	116687
Full Name	deoxyribonuclease 1-like 3
General protein information	Preferred Names deoxyribonuclease 1-like 3 Names deoxyribonuclease gamma DNase I-like 3 DNase gamma DNaseY deoxyribonuclease I-like 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p14
Summary	Ca2+/Mg2+ dependent endonuclease that catalyzes cleavage of both single and double stranded DNA; may play a role in apoptotic DNA fragmentation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053907.1	NP_446359.1	deoxyribonuclease gamma precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dnase1l3	NP_031896.3
Homo sapiens (human)	DNASE1L3	NP_004935.1
Pan troglodytes (chimpanzee)	DNASE1L3	XP_001174037.1
Canis lupus familiaris (dog)	DNASE1L3	XP_533780.1
Danio rerio (zebrafish)	dnase1l3	NP_998318.2
Rattus norvegicus (Norway rat)	Dnase1l3	NP_446359.1
Danio rerio (zebrafish)	dnase1l3l	NP_001002403.1
	DNASE1L3	XP_001097104.1
Bos taurus (cattle)	DNASE1L3	NP_001192653.1
Gallus gallus (chicken)	DNASE1L3	NP_001029987.1
Xenopus tropicalis (tropical clawed frog)	dnase1l3	NP_988919.1

Gene Ontology
Bibliography

Related articles in PubMed

Host DNases prevent vascular occlusion by neutrophil extracellular traps.
Jim?nez-Alc?zar M, Rangaswamy C, Panda R, Bitterling J, Simsek YJ, Long AT, Bilyy R, Krenn V, Renn? C, Renn? T, Kluge S, Panzer U, Mizuta R, Mannherz HG, Kitamura D, Herrmann M, Napirei M, Fuchs TA
Science (New York, N.Y.)358(6367)1202-1206(2017 12)

Apoptotic DNA fragmentation may be a cooperative activity between caspase-activated deoxyribonuclease and the poly(ADP-ribose) polymerase-regulated DNAS1L3, an endoplasmic reticulum-localized endonuclease that translocates to the nucleus during apoptosis.
Errami Y, Naura AS, Kim H, Ju J, Suzuki Y, El-Bahrawy AH, Ghonim MA, Hemeida RA, Mansy MS, Zhang J, Xu M, Smulson ME, Brim H, Boulares AH
The Journal of biological chemistry288(5)3460-8(2013 Feb)

Differential DNases are selectively used in neuronal apoptosis depending on the differentiation state.
Shiokawa D, Tanuma S
Cell death and differentiation11(10)1112-20(2004 Oct)

Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes.
Higami Y, Tsuchiya T, To K, Chiba T, Yamaza H, Shiokawa D, Tanuma S, Shimokawa I
Cell and tissue research316(3)403-7(2004 Jun)

Involvement of DNase gamma in apoptosis associated with myogenic differentiation of C2C12 cells.
Shiokawa D, Kobayashi T, Tanuma S
The Journal of biological chemistry277(34)31031-7(2002 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DNase gamma is suggested to be involved in naturally occurring apoptosis in developing nervous systems
Title: Differential DNases are selectively used in neuronal apoptosis depending on the differentiation state.

DNase gamma plays an important role in Fas-independent apoptotic DNA fragmentation in hepatocytes.
Title: Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes.

The following Dnase1l3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnase1l3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11024	NM_053907.1 Latest version!	Rattus norvegicus deoxyribonuclease 1-like 3 (Dnase1l3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnase1l3 ( NM_053907.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446359.1 Rattus norvegicus deoxyribonuclease 1-like 3 (Dnase1l3), mRNA.

Price & Availability↑

CloneID	ORa11024
Clone ID Related Accession (Same CDS sequence)	NM_053907.1
Accession Version	NM_053907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	deoxyribonuclease gamma precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U75689.1. On Jun 26, 2012 this sequence version replaced XM_003751446.1. ##Evidence-Data-START## Transcript exon combination :: FQ231976.1, FQ230990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCTGT ACCCAGCTTC CCCATACCTG GCCTCCCTGC TACTCTTCAT CCTTGCCCTT 
CATGGTGCCC TGTCCCTGAG GCTCTGCTCC TTCAATGTGA GGTCCTTTGG AGAGAGCAAG 
AAGGAAAACC ACAATGCCAT GGATATCATT GTGAAGATCA TCAAACGCTG CGACCTCATA 
CTGCTGATGG AAATCAAGGA CAGCAACAAC AACATCTGTC CCATGCTGAT GGAGAAGCTG 
AATGGAAACT CACGAAGAAG CACGACATAC AACTACGTGA TTAGCTCTCG GCTTGGAAGA 
AACACATATA AAGAACAGTA TGCCTTCCTC TACAAGGAGA AGCTGGTGTC TGTGAAGGCA 
AAATACCTCT ACCATGACTA TCAGGATGGA GACACAGACG TGTTTTCCAG GGAGCCCTTT 
GTGGTTTGGT TCCAGGCGCC CTTCACTGCT GCCAAGGACT TCGTGATTGT CCCCTTGCAC 
ACAACTCCTG AAACCTCCGT TAAAGAGATA GATGAGCTGG CTGACGTCTA CACGGATGTG 
AGAAGACGAT GGAAGGCAGA GAATTTCATC TTCATGGGTG ATTTCAATGC TGGCTGCAGC 
TACGTCCCCA AGAAGGCCTG GAAGAACATC CGTTTGAGGA CAGACCCCAA CTTTGTTTGG 
CTGATTGGGG ACCAAGAGGA CACCACGGTC AAGAAGAGCA CCAGCTGTGC CTATGACAGG 
ATTGTGCTTC GCGGACAAGA GATAGTCAAC TCTGTGGTTC CCCGCTCCAG TGGCGTCTTT 
GACTTTCAGA AAGCTTATGA GTTGTCTGAA GAGGAGGCCC TGGATGTCAG TGACCACTTT 
CCAGTTGAGT TTAAGCTACA GAGTTCAAGA GCCTTCACCA ACAGCCGAAA ATCTGTTTCT 
CTAAAGAAAA AGAAAAAAGG CAGTCGCTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446359.1
CDS	203..1135
Translation	MSLYPASPYLASLLLFILALHGALSLRLCSFNVRSFGESKKENHNAMDIIVKIIKRCDLILLMEIKDSNNNICPMLMEKLNGNSRRSTTYNYVISSRLGRNTYKEQYAFLYKEKLVSVKAKYLYHDYQDGDTDVFSREPFVVWFQAPFTAAKDFVIVPLHTTPETSVKEIDELADVYTDVRRRWKAENFIFMGDFNAGCSYVPKKAWKNIRLRTDPNFVWLIGDQEDTTVKKSTSCAYDRIVLRGQEIVNSVVPRSSGVFDFQKAYELSEEEALDVSDHFPVEFKLQSSRAFTNSRKSVSLKKKKKGSRS

Target ORF information:

RefSeq Version	NM_053907.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus deoxyribonuclease 1-like 3 (Dnase1l3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053907.1

ORF Insert Sequence:

ATGTCCCTGT ACCCAGCTTC CCCATACCTG GCCTCCCTGC TACTCTTCAT CCTTGCCCTT 
CATGGTGCCC TGTCCCTGAG GCTCTGCTCC TTCAATGTGA GGTCCTTTGG AGAGAGCAAG 
AAGGAAAACC ACAATGCCAT GGATATCATT GTGAAGATCA TCAAACGCTG CGACCTCATA 
CTGCTGATGG AAATCAAGGA CAGCAACAAC AACATCTGTC CCATGCTGAT GGAGAAGCTG 
AATGGAAACT CACGAAGAAG CACGACATAC AACTACGTGA TTAGCTCTCG GCTTGGAAGA 
AACACATATA AAGAACAGTA TGCCTTCCTC TACAAGGAGA AGCTGGTGTC TGTGAAGGCA 
AAATACCTCT ACCATGACTA TCAGGATGGA GACACAGACG TGTTTTCCAG GGAGCCCTTT 
GTGGTTTGGT TCCAGGCGCC CTTCACTGCT GCCAAGGACT TCGTGATTGT CCCCTTGCAC 
ACAACTCCTG AAACCTCCGT TAAAGAGATA GATGAGCTGG CTGACGTCTA CACGGATGTG 
AGAAGACGAT GGAAGGCAGA GAATTTCATC TTCATGGGTG ATTTCAATGC TGGCTGCAGC 
TACGTCCCCA AGAAGGCCTG GAAGAACATC CGTTTGAGGA CAGACCCCAA CTTTGTTTGG 
CTGATTGGGG ACCAAGAGGA CACCACGGTC AAGAAGAGCA CCAGCTGTGC CTATGACAGG 
ATTGTGCTTC GCGGACAAGA GATAGTCAAC TCTGTGGTTC CCCGCTCCAG TGGCGTCTTT 
GACTTTCAGA AAGCTTATGA GTTGTCTGAA GAGGAGGCCC TGGATGTCAG TGACCACTTT 
CCAGTTGAGT TTAAGCTACA GAGTTCAAGA GCCTTCACCA ACAGCCGAAA ATCTGTTTCT 
CTAAAGAAAA AGAAAAAAGG CAGTCGCTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Host DNases prevent vascular occlusion by neutrophil extracellular traps.
Science (New York, N.Y.)358(6367)1202-1206(2017 12)
Jim?nez-Alc?zar M,Rangaswamy C,Panda R,Bitterling J,Simsek YJ,Long AT,Bilyy R,Krenn V,Renn? C,Renn? T,Kluge S,Panzer U,Mizuta R,Mannherz HG,Kitamura D,Herrmann M,Napirei M,Fuchs TA

Apoptotic DNA fragmentation may be a cooperative activity between caspase-activated deoxyribonuclease and the poly(ADP-ribose) polymerase-regulated DNAS1L3, an endoplasmic reticulum-localized endonuclease that translocates to the nucleus during apoptosis.
The Journal of biological chemistry288(5)3460-8(2013 Feb)
Errami Y,Naura AS,Kim H,Ju J,Suzuki Y,El-Bahrawy AH,Ghonim MA,Hemeida RA,Mansy MS,Zhang J,Xu M,Smulson ME,Brim H,Boulares AH

Differential DNases are selectively used in neuronal apoptosis depending on the differentiation state.
Cell death and differentiation11(10)1112-20(2004 Oct)
Shiokawa D,Tanuma S

Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes.
Cell and tissue research316(3)403-7(2004 Jun)
Higami Y,Tsuchiya T,To K,Chiba T,Yamaza H,Shiokawa D,Tanuma S,Shimokawa I

Involvement of DNase gamma in apoptosis associated with myogenic differentiation of C2C12 cells.
The Journal of biological chemistry277(34)31031-7(2002 Aug)
Shiokawa D,Kobayashi T,Tanuma S