F2rl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	F2rl1
Entrez Gene ID	116677
Full Name	F2R like trypsin receptor 1
Synonyms	Par-2,Par2
General protein information	Preferred Names F2R like trypsin receptor 1 Names proteinase-activated receptor 2 Proteinase-activated receptor-2, G protein-coupled receptor 11 coagulation factor II (thrombin) receptor-like 1 coagulation factor II receptor-like 1 thrombin receptor-like 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	G-coupled protein receptor that is activated by coagulation factor II and other serine proteases; may play a role in regulation of exocrine secretion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053897.2	NP_446349.1	proteinase-activated receptor 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	F2RL1	NP_005233.3
	F2RL1	XP_001106263.1
Mus musculus (house mouse)	F2rl1	NP_032000.3
Gallus gallus (chicken)	F2RL1	NP_001012626.1
Danio rerio (zebrafish)	f2rl1.2	NP_001092248.1
Canis lupus familiaris (dog)	F2RL1	NP_001139463.1
Rattus norvegicus (Norway rat)	F2rl1	NP_446349.1
Pan troglodytes (chimpanzee)	F2RL1	XP_001145175.1
Xenopus tropicalis (tropical clawed frog)	f2rl1	XP_004910449.1
Bos taurus (cattle)	F2RL1	NP_001039748.1

Gene Ontology
Bibliography

Related articles in PubMed

Stress induces more serious barrier dysfunction in follicle-associated epithelium than villus epithelium involving mast cells and protease-activated receptor-2.
Zhang L, Song J, Bai T, Qian W, Hou XH
Scientific reports7(1)4950(2017 Jul)

Structural insight into allosteric modulation of protease-activated receptor 2.
Cheng RKY, Fiez-Vandal C, Schlenker O, Edman K, Aggeler B, Brown DG, Brown GA, Cooke RM, Dumelin CE, Dor? AS, Geschwindner S, Grebner C, Hermansson NO, Jazayeri A, Johansson P, Leong L, Prihandoko R, Rappas M, Soutter H, Snijder A, Sundstr?m L, Tehan B, Thornton P, Troast D, Wiggin G, Zhukov A, Marshall FH, Dekker N
Nature545(7652)112-115(2017 05)

Proteinase-Activated Receptor-2 Sensitivity of Amplified TRPA1 Activity in Skeletal Muscle Afferent Nerves and Exercise Pressor Reflex in Rats with Femoral Artery Occlusion.
Xing J, Li J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology44(1)163-171(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of this study suggest that PAR2 plays an important role in mediating renal injury induced by glomerulosclerosis.
Title: Role of Protease-Activated Receptor 2 in Regulating Focal Segmental Glomerulosclerosis.

PAR1 and PAR2 play a critical role in Ang II-induced adventitial fibroblasts activation, and this may contribute to adventitia-related pathological changes.
Title: Protease-activated receptor 1 and 2 contribute to angiotensin II-induced activation of adventitial fibroblasts from rat aorta.

tryptase may directly damage IEC6 cells via PAR-2 and the downstream activation of ERK, and demonstrate that the signaling pathway requires beta-arrestin-2.
Title: Intestinal mucosal injury induced by tryptase-activated protease-activated receptor 2 requires ?-arrestin-2 in vitro.

XZP significantly alleviates breast cancer-induced bone pain by inhibiting the PAR2 signaling pathway.
Title: Topical treatment with Xiaozheng Zhitong Paste alleviates bone cancer pain by inhibiting proteinase-activated receptor?2 signaling pathway.

PAR2 stimulation of NO production via an additional pathway that targets phosphorylation of Ser1177-eNOS suggests a regulatory mechanism for sustaining agonist-mediated vasodilation in metabolic syndrome.
Title: Enhanced Nitric Oxide Synthase Activation via Protease-Activated Receptor 2 Is Involved in the Preserved Vasodilation in Aortas from Metabolic Syndrome Rats.

The following F2rl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F2rl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13058	NM_053897.2 Latest version!	Rattus norvegicus F2R like trypsin receptor 1 (F2rl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F2rl1 ( NM_053897.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446349.1 Rattus norvegicus F2R like trypsin receptor 1 (F2rl1), mRNA.

Price & Availability↑

CloneID	ORa13058
Clone ID Related Accession (Same CDS sequence)	NM_053897.2
Accession Version	NM_053897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	proteinase-activated receptor 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC099765.1. On Jul 31, 2005 this sequence version replaced NM_053897.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC099765.1, U61373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689596 [ECO:0000348] ##Evidence-Data-END##

ATGCGAAGTC TCAGCCTGGC GTGGCTGCTG GGAGGTATCA CCCTTCTGGC GGCCTCGGCC 
TCCTGCAACC GGACCGTGAA TGCACCGGGA CCCAACAGTA AAGGGAGAAG TCTGATTGGC 
AGATTGGACA CGCCGCCTCC CATCACTGGG AAAGGGGCTC CAGTTGAACC AGGCTTTTCC 
GTTGATGAAT TCTCTGCATC CGTCCTCACC GGGAAGCTGA CCACCGTCTT TCTCCCGGTC 
ATCTACATCA TTGTCTTTGT AATTGGTTTG CCCAGTAATG GTATGGCCCT CTGGGTCTTC 
TTCTTCCGAA CGAAGAAGAA GCACCCTGCT GTGATTTACA TGGCCAACCT GGCCTTGGCA 
GACCTCCTCT CTGTCATCTG GTTCCCCCTG AAGATCTCCT ACCACCTCCA TGGCAACGAC 
TGGACCTATG GGGATGCGCT CTGCAAGGTG CTCATTGGCT TTTTCTACGG CAATATGTAC 
TGCTCCATCC TTTTCATGAC CTGCCTCAGC GTGCAGAGGT ACTGGGTGAT CGTGAACCCC 
ATGGGACACT CCAGGAAGAG GGCCAACATC GCTGTTGGCG TCTCCCTGGC CATCTGGCTC 
CTGATTTTTC TGGTCACCAT CCCTCTGTAC GTCATGAGGC AGACCATCTA CATTCCAGCC 
TTGAACATCA CCACCTGTCA CGACGTGCTG CCCGAGGAGG TCCTGGTGGG GGACATGTTC 
AGTTACTTCC TCTCCCTGGC CATTGGAGTC TTTCTGTTCC CAGCCCTCCT TACTGCGTCT 
GCCTACGTGC TCATGATCAA AACGCTCCGT TCCTCCGCCA TGGACGAGCA CTCGGAGAAG 
AAAAGGCGGA GGGCTATCCG CCTCATCATC ACGGTGCTGT CCATGTACTT CATCTGCTTC 
GCTCCCAGCA ACGTGCTGCT CGTCGTGCAT TATTTCCTCA TCAAAAGCCA GAGGCAGAGC 
CACGTCTACG CCCTCTACCT CGTCGCCCTC TGCCTGTCCA CCCTCAACAG CTGCATAGAC 
CCCTTTGTCT ACTACTTTGT TTCGAAAGAT TTCAGGGACC AGGCCAGAAA CGCGCTCCTC 
TGCCGAAGCG TCCGCACCGT GAAACGCATG CAGATATCGC TCACCTCCAA CAAGTTCTCC 
AGGAAATCCA GCTCTTACTC CTCCAGCTCA ACCAGTGTTA AAACCTCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446349.1
CDS	88..1281
Translation	MRSLSLAWLLGGITLLAASASCNRTVNAPGPNSKGRSLIGRLDTPPPITGKGAPVEPGFSVDEFSASVLTGKLTTVFLPVIYIIVFVIGLPSNGMALWVFFFRTKKKHPAVIYMANLALADLLSVIWFPLKISYHLHGNDWTYGDALCKVLIGFFYGNMYCSILFMTCLSVQRYWVIVNPMGHSRKRANIAVGVSLAIWLLIFLVTIPLYVMRQTIYIPALNITTCHDVLPEEVLVGDMFSYFLSLAIGVFLFPALLTASAYVLMIKTLRSSAMDEHSEKKRRRAIRLIITVLSMYFICFAPSNVLLVVHYFLIKSQRQSHVYALYLVALCLSTLNSCIDPFVYYFVSKDFRDQARNALLCRSVRTVKRMQISLTSNKFSRKSSSYSSSSTSVKTSY

Target ORF information:

RefSeq Version	NM_053897.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus F2R like trypsin receptor 1 (F2rl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053897.2

ORF Insert Sequence:

ATGCGAAGTC TCAGCCTGGC GTGGCTGCTG GGAGGTATCA CCCTTCTGGC GGCCTCGGCC 
TCCTGCAACC GGACCGTGAA TGCACCGGGA CCCAACAGTA AAGGGAGAAG TCTGATTGGC 
AGATTGGACA CGCCGCCTCC CATCACTGGG AAAGGGGCTC CAGTTGAACC AGGCTTTTCC 
GTTGATGAAT TCTCTGCATC CGTCCTCACC GGGAAGCTGA CCACCGTCTT TCTCCCGGTC 
ATCTACATCA TTGTCTTTGT AATTGGTTTG CCCAGTAATG GTATGGCCCT CTGGGTCTTC 
TTCTTCCGAA CGAAGAAGAA GCACCCTGCT GTGATTTACA TGGCCAACCT GGCCTTGGCA 
GACCTCCTCT CTGTCATCTG GTTCCCCCTG AAGATCTCCT ACCACCTCCA TGGCAACGAC 
TGGACCTATG GGGATGCGCT CTGCAAGGTG CTCATTGGCT TTTTCTACGG CAATATGTAC 
TGCTCCATCC TTTTCATGAC CTGCCTCAGC GTGCAGAGGT ACTGGGTGAT CGTGAACCCC 
ATGGGACACT CCAGGAAGAG GGCCAACATC GCTGTTGGCG TCTCCCTGGC CATCTGGCTC 
CTGATTTTTC TGGTCACCAT CCCTCTGTAC GTCATGAGGC AGACCATCTA CATTCCAGCC 
TTGAACATCA CCACCTGTCA CGACGTGCTG CCCGAGGAGG TCCTGGTGGG GGACATGTTC 
AGTTACTTCC TCTCCCTGGC CATTGGAGTC TTTCTGTTCC CAGCCCTCCT TACTGCGTCT 
GCCTACGTGC TCATGATCAA AACGCTCCGT TCCTCCGCCA TGGACGAGCA CTCGGAGAAG 
AAAAGGCGGA GGGCTATCCG CCTCATCATC ACGGTGCTGT CCATGTACTT CATCTGCTTC 
GCTCCCAGCA ACGTGCTGCT CGTCGTGCAT TATTTCCTCA TCAAAAGCCA GAGGCAGAGC 
CACGTCTACG CCCTCTACCT CGTCGCCCTC TGCCTGTCCA CCCTCAACAG CTGCATAGAC 
CCCTTTGTCT ACTACTTTGT TTCGAAAGAT TTCAGGGACC AGGCCAGAAA CGCGCTCCTC 
TGCCGAAGCG TCCGCACCGT GAAACGCATG CAGATATCGC TCACCTCCAA CAAGTTCTCC 
AGGAAATCCA GCTCTTACTC CTCCAGCTCA ACCAGTGTTA AAACCTCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stress induces more serious barrier dysfunction in follicle-associated epithelium than villus epithelium involving mast cells and protease-activated receptor-2.
Scientific reports7(1)4950(2017 Jul)
Zhang L,Song J,Bai T,Qian W,Hou XH

Structural insight into allosteric modulation of protease-activated receptor 2.
Nature545(7652)112-115(2017 05)
Cheng RKY,Fiez-Vandal C,Schlenker O,Edman K,Aggeler B,Brown DG,Brown GA,Cooke RM,Dumelin CE,Dor? AS,Geschwindner S,Grebner C,Hermansson NO,Jazayeri A,Johansson P,Leong L,Prihandoko R,Rappas M,Soutter H,Snijder A,Sundstr?m L,Tehan B,Thornton P,Troast D,Wiggin G,Zhukov A,Marshall FH,Dekker N

Proteinase-Activated Receptor-2 Sensitivity of Amplified TRPA1 Activity in Skeletal Muscle Afferent Nerves and Exercise Pressor Reflex in Rats with Femoral Artery Occlusion.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology44(1)163-171(2017)
Xing J,Li J