Aimp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aimp1
Entrez Gene ID	114632
Full Name	aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
Synonyms	Emap2,Scye1
General protein information	Preferred Names aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 Names aminoacyl tRNA synthase complex-interacting multifunctional protein 1 endothelial monocyte activating polypeptide 2 small inducible cytokine subfamily E, member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q43
Summary	cytokine with proinflammatory and antiangiogenic properties [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053757.1	NP_446209.1	aminoacyl tRNA synthase complex-interacting multifunctional protein 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Aimp1	NP_031952.2
Rattus norvegicus (Norway rat)	Aimp1	NP_446209.1
Drosophila melanogaster (fruit fly)	CG8235	NP_610426.2
Pan troglodytes (chimpanzee)	AIMP1	XP_001170763.2
Homo sapiens (human)	AIMP1	NP_001135888.1
Bos taurus (cattle)	AIMP1	NP_001030190.2
Canis lupus familiaris (dog)	AIMP1	XP_545016.2
Gallus gallus (chicken)	AIMP1	XP_420496.3
Danio rerio (zebrafish)	aimp1	NP_001039316.1
Xenopus tropicalis (tropical clawed frog)	aimp1	NP_001017050.2

Gene Ontology
Bibliography

Related articles in PubMed

Endothelial-monocyte-activating polypeptide II induces rat C6 glioma cell apoptosis via the mitochondrial pathway.
Liu LB, Xie H, Xue YX, Liu YH, Li Z, Wang P
Biochemical and biophysical research communications457(4)595-601(2015 Feb)

Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton.
Forster F, Paster W, Supper V, Schatzlmaier P, Sunzenauer S, Ostler N, Saliba A, Eckerstorfer P, Britzen-Laurent N, Sch?tz G, Schmid JA, Zlabinger GJ, Naschberger E, St?rzl M, Stockinger H
Journal of immunology (Baltimore, Md. : 1950)192(2)771-81(2014 Jan)

Signal mechanisms underlying low-dose endothelial monocyte-activating polypeptide-II-induced opening of the blood-tumor barrier.
Li Z, Liu YH, Xue YX, Liu LB, Wang P
Journal of molecular neuroscience : MN48(1)291-301(2012 Sep)

Mechanisms for endothelial monocyte-activating polypeptide-II-induced opening of the blood-tumor barrier.
Li Z, Liu YH, Xue YX, Liu LB, Xie H
Journal of molecular neuroscience : MN47(2)408-17(2012 Jun)

Endothelial-monocyte activating polypeptide II disrupts alveolar epithelial type II to type I cell transdifferentiation.
Chen Y, Legan SK, Mahan A, Thornton J, Xu H, Schwarz MA
Respiratory research131(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EMAP2 decreases cell viability of rat C6 glioma cells. EMAP2 induces rat C6 glioma cells apoptosis via the mitochondrial pathway.
Title: Endothelial-monocyte-activating polypeptide II induces rat C6 glioma cell apoptosis via the mitochondrial pathway.

EMAP-II induces blood tumor barrier opening via the RhoA/Rho kinase/PKC-alpha/beta signaling pathways, however, PKC-zeta is involved in this process by other mechanisms.
Title: Signal mechanisms underlying low-dose endothelial monocyte-activating polypeptide-II-induced opening of the blood-tumor barrier.

EMAP-II increases permeability through alpha-ATP synthase on the surface of brain microvascular endothelial cells.
Title: Mechanisms for endothelial monocyte-activating polypeptide-II-induced opening of the blood-tumor barrier.

The findings demonstrate that small inducible cytokine subfamily E member 1 interferes with alveolar cell transdifferentiation resulting in a proliferating non-myofibroblast cell.
Title: Endothelial-monocyte activating polypeptide II disrupts alveolar epithelial type II to type I cell transdifferentiation.

This study demonstrates for the first time that EMAP II increases the permeability of blood-tumor barrier selectively, and the possible mechanism is associated with the down-regulation of ZO-1, occludin and claudin-5.
Title: Endothelial-monocyte-activating polypeptide II increases blood-tumor barrier permeability by down-regulating the expression levels of tight junction associated proteins.

The following Aimp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aimp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10533	NM_053757.1 Latest version!	Rattus norvegicus aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 (Aimp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aimp1 ( NM_053757.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446209.1 Rattus norvegicus aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 (Aimp1), mRNA.

Price & Availability↑

CloneID	ORa10533
Clone ID Related Accession (Same CDS sequence)	NM_053757.1
Accession Version	NM_053757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	aminoacyl tRNA synthase complex-interacting multifunctional protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098675.1. On Sep 19, 2005 this sequence version replaced XM_342344.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098675.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATTTTCT GCCGATTCTG GGGAAAGATG GCCACCAATG ATGCTGTTCT GAAGCGGCTG 
GAGCAGAAAG GCGCAGAGGC GGATCAGATC ATTGAGTATC TCAAGCAGCA GGTTGCTCTT 
CTCAAGGAGA AAGCAATTTT GCAGGCAACT TTGAGAGAAG AAAAGAAACT TCAAGTTGAA 
AATGCTAAAC TGAAAAAAGA AATAGAAGAA CTAAAGCAAG AGTTGATTCA GGCAGAAATT 
CATAATGGAG TGAAGCAGGT GCCTGTCCCA TCGAGTTCTT CACTGGAGAC TCATTGTTCC 
GTTTCTGAAA GTGTGACTCG GTCTGCATCA GTAACAACCA CCACCATGAC CGGAGGGGGA 
GGGGAAGAAA AGAAGGTAAA AGAGAAGACG GAAAAGAAAG GAGAGAAAAA GGAGAAGAAG 
CAGCAGTCAG CGGCAGCAAG TGCTGATTCG AAGCCTGTCG ACGTATCACG CCTGGATCTT 
CGAATTGGAT GTATTGTCAC TGCCAAAAAA CACCCGGATG CAGATTCGTT GTATGTGGAG 
GAAGTCGATG TGGGAGAAGC AGCTCCACGG ACGGTCGTCA GCGGGCTGGT GAATCATGTC 
CCTCTAGAAC AGATGCAAAA CCGGATGGTG GTTTTGCTTT GTAATCTGAA GCCTGCAAAA 
ATGCGGGGAG TGCTGTCCCA AGCAATGGTG ATGTGCGCCA GTTCACCAGA GAAAGTGGAG 
ATTTTGGCCC CTCCCAACGG GTCTGTTCCT GGAGACAGAA TTACTTTTGA TGCTTTCCCT 
GGAGAGCCTG ACAAGGAGCT GAACCCTAAG AAGAAGATCT GGGAGCAGAT CCAGCCTGAC 
CTGCACACCA ATGATGAGTG TGTAGCCACA TACAAAGGAG CTCCCTTTGA GGTGAAGGGG 
AAGGGAGTTT GCAGGGCCCA AACCATGGCC AACAGCGGAA TTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446209.1
CDS	46..993
Translation	MIFCRFWGKMATNDAVLKRLEQKGAEADQIIEYLKQQVALLKEKAILQATLREEKKLQVENAKLKKEIEELKQELIQAEIHNGVKQVPVPSSSSLETHCSVSESVTRSASVTTTTMTGGGGEEKKVKEKTEKKGEKKEKKQQSAAASADSKPVDVSRLDLRIGCIVTAKKHPDADSLYVEEVDVGEAAPRTVVSGLVNHVPLEQMQNRMVVLLCNLKPAKMRGVLSQAMVMCASSPEKVEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGAPFEVKGKGVCRAQTMANSGIK

Target ORF information:

RefSeq Version	NM_053757.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 (Aimp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053757.1

ORF Insert Sequence:

ATGATTTTCT GCCGATTCTG GGGAAAGATG GCCACCAATG ATGCTGTTCT GAAGCGGCTG 
GAGCAGAAAG GCGCAGAGGC GGATCAGATC ATTGAGTATC TCAAGCAGCA GGTTGCTCTT 
CTCAAGGAGA AAGCAATTTT GCAGGCAACT TTGAGAGAAG AAAAGAAACT TCAAGTTGAA 
AATGCTAAAC TGAAAAAAGA AATAGAAGAA CTAAAGCAAG AGTTGATTCA GGCAGAAATT 
CATAATGGAG TGAAGCAGGT GCCTGTCCCA TCGAGTTCTT CACTGGAGAC TCATTGTTCC 
GTTTCTGAAA GTGTGACTCG GTCTGCATCA GTAACAACCA CCACCATGAC CGGAGGGGGA 
GGGGAAGAAA AGAAGGTAAA AGAGAAGACG GAAAAGAAAG GAGAGAAAAA GGAGAAGAAG 
CAGCAGTCAG CGGCAGCAAG TGCTGATTCG AAGCCTGTCG ACGTATCACG CCTGGATCTT 
CGAATTGGAT GTATTGTCAC TGCCAAAAAA CACCCGGATG CAGATTCGTT GTATGTGGAG 
GAAGTCGATG TGGGAGAAGC AGCTCCACGG ACGGTCGTCA GCGGGCTGGT GAATCATGTC 
CCTCTAGAAC AGATGCAAAA CCGGATGGTG GTTTTGCTTT GTAATCTGAA GCCTGCAAAA 
ATGCGGGGAG TGCTGTCCCA AGCAATGGTG ATGTGCGCCA GTTCACCAGA GAAAGTGGAG 
ATTTTGGCCC CTCCCAACGG GTCTGTTCCT GGAGACAGAA TTACTTTTGA TGCTTTCCCT 
GGAGAGCCTG ACAAGGAGCT GAACCCTAAG AAGAAGATCT GGGAGCAGAT CCAGCCTGAC 
CTGCACACCA ATGATGAGTG TGTAGCCACA TACAAAGGAG CTCCCTTTGA GGTGAAGGGG 
AAGGGAGTTT GCAGGGCCCA AACCATGGCC AACAGCGGAA TTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Endothelial-monocyte-activating polypeptide II induces rat C6 glioma cell apoptosis via the mitochondrial pathway.
Biochemical and biophysical research communications457(4)595-601(2015 Feb)
Liu LB,Xie H,Xue YX,Liu YH,Li Z,Wang P

Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton.
Journal of immunology (Baltimore, Md. : 1950)192(2)771-81(2014 Jan)
Forster F,Paster W,Supper V,Schatzlmaier P,Sunzenauer S,Ostler N,Saliba A,Eckerstorfer P,Britzen-Laurent N,Sch?tz G,Schmid JA,Zlabinger GJ,Naschberger E,St?rzl M,Stockinger H

Signal mechanisms underlying low-dose endothelial monocyte-activating polypeptide-II-induced opening of the blood-tumor barrier.
Journal of molecular neuroscience : MN48(1)291-301(2012 Sep)
Li Z,Liu YH,Xue YX,Liu LB,Wang P

Mechanisms for endothelial monocyte-activating polypeptide-II-induced opening of the blood-tumor barrier.
Journal of molecular neuroscience : MN47(2)408-17(2012 Jun)
Li Z,Liu YH,Xue YX,Liu LB,Xie H

Endothelial-monocyte activating polypeptide II disrupts alveolar epithelial type II to type I cell transdifferentiation.
Respiratory research131(2012 Jan)
Chen Y,Legan SK,Mahan A,Thornton J,Xu H,Schwarz MA