Abcg5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Abcg5
Entrez Gene ID	114628
Full Name	ATP binding cassette subfamily G member 5
General protein information	Preferred Names ATP binding cassette subfamily G member 5 Names ATP-binding cassette sub-family G member 5 ATP-binding cassette sub-family G (WHITE) member 5 (sterolin 1) ATP-binding cassette, sub-family G (WHITE), member 5 (sterolin 1) ATP-binding cassette, subfamily G (WHITE), member 5 sterolin 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q12
Summary	ATP-transporter family member that may regulate absorption of dietary cholesterol; human homolog is mutated in sitosterolemia, a disease of hyperabsorption of cholesterol and other sterols [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053754.2	NP_446206.2	ATP-binding cassette sub-family G member 5
XM_017593976.1	XP_017449465.1	ATP-binding cassette sub-family G member 5 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ABCG5	NP_071881.1
Canis lupus familiaris (dog)	ABCG5	XP_538475.1
Rattus norvegicus (Norway rat)	Abcg5	NP_446206.2
Danio rerio (zebrafish)	abcg5	NP_001122162.1
Bos taurus (cattle)	ABCG5	NP_001019718.1
Mus musculus (house mouse)	Abcg5	NP_114090.1
Drosophila melanogaster (fruit fly)	CG11069	NP_651307.2
Xenopus tropicalis (tropical clawed frog)	abcg5	NP_001120613.1
Pan troglodytes (chimpanzee)	ABCG5	XP_003309098.1
	ABCG5	XP_001111277.1
Gallus gallus (chicken)	ABCG5	XP_419457.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002051	XP_320996.5

Gene Ontology
Bibliography

Related articles in PubMed

Genetic Control of Serum Marinobufagenin in the Spontaneously Hypertensive Rat and the Relationship to Blood Pressure.
Dmitrieva RI, Cranford SM, Doris PA
Journal of the American Heart Association6(10)(2017 Oct)

Crystal structure of the human sterol transporter ABCG5/ABCG8.
Lee JY, Kinch LN, Borek DM, Wang J, Wang J, Urbatsch IL, Xie XS, Grishin NV, Cohen JC, Otwinowski Z, Hobbs HH, Rosenbaum DM
Nature533(7604)561-4(2016 05)

Novel candidate genes for impaired nephron development in a rat model with inherited nephron deficit and albuminuria.
Herlan L, Schulz A, Schulte L, Schulz H, H?bner N, Kreutz R
Clinical and experimental pharmacology & physiology42(10)1051-8(2015 Oct)

Influence of dietary sugar on cholesterol and bile acid metabolism in?the rat: Marked reduction of hepatic Abcg5/8 expression following sucrose ingestion.
Apro J, Beckman L, Angelin B, Rudling M
Biochemical and biophysical research communications461(4)592-7(2015 Jun)

SHRSP/Izm and WKY/NCrlCrlj rats having a missense mutation in Abcg5 deposited plant sterols in the body, but did not change their biliary secretion and lymphatic absorption-comparison with Jcl:Wistar and WKY/Izm rats.
Kato M, Ito Y, Tanaka Y, Sato M, Imaizumi K, Inoue N, Ikeda I
Bioscience, biotechnology, and biochemistry76(4)660-4(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In contrast, the marked reduction of hepatic Abcg5/8 observed is an effect of the high sugar content of the diets.
Title: Influence of dietary sugar on cholesterol and bile acid metabolism in?the rat: Marked reduction of hepatic Abcg5/8 expression following sucrose ingestion.

A missense mutation in Abcg5 in hypertensive rats results in deposition of plant sterols in the body, but does not change their biliary secretion and lymphatic absorption.
Title: SHRSP/Izm and WKY/NCrlCrlj rats having a missense mutation in Abcg5 deposited plant sterols in the body, but did not change their biliary secretion and lymphatic absorption-comparison with Jcl:Wistar and WKY/Izm rats.

Data reveal marked utilization of an Abcg5/Abcg8-independent mechanism for basal biliary cholesterol secretion in rats during lactation, but a dependence on Abcg5/g8 for maximal biliary cholesterol secretion.
Title: ABCG5/ABCG8-independent biliary cholesterol excretion in lactating rats.

Mutation of Abcg5 in stroke-prone spontaneously hypertensive rats, compared with Wistar rats, did not influence the ability for intestinal sitosterol absorption regardless of the dose.
Title: Missense mutation of Abcg5 in stroke-prone spontaneously hypertensive rats does not influence lymphatic sitosterol absorption regardless of the dose: comparison with Wistar rats.

These observations suggest that a mutation in Abcg5 does not greatly influence intestinal absorption of plant sterols in SHRSP rats, at least in comparison with Wistar rats.
Title: Missense mutation in Abcg5 in SHRSP rats does not accelerate intestinal absorption of plant sterols: comparison with Wistar rats.

The following Abcg5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Abcg5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10524	NM_053754.2 Latest version!	Rattus norvegicus ATP binding cassette subfamily G member 5 (Abcg5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
ORa61510	XM_017593976.1 Latest version!	Rattus norvegicus ATP binding cassette subfamily G member 5 (Abcg5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Abcg5 ( NM_053754.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446206.2 Rattus norvegicus ATP binding cassette subfamily G member 5 (Abcg5), mRNA.

Price & Availability↑

CloneID	ORa10524
Clone ID Related Accession (Same CDS sequence)	NM_053754.2
Accession Version	NM_053754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-binding cassette sub-family G member 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF312714.3. On or before Dec 17, 2003 this sequence version replaced XM_347385.1, NM_053754.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF312714.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906357, SAMN01906358 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGAGC TGCCCTTTCT GAGTCCAGAG GGAGCCAGAG GGCCTCACAA CAACAGAGGG 
TCTCAGAGCT CCCTGGAGGA AGGCTCAGTT ACAGGCTCAG AGGCTCGGCA CAGCTTAGGT 
GTCCTGAATG TGTCCTTCAG CGTCAGCAAC CGTGTCGGGC CCTGGTGGAA CATCAAATCA 
TGCCAGCAGA AGTGGGACAG GAAAATCCTC AAAGATGTCT CCTTGTACAT CGAGAGTGGC 
CAGACCATGT GCATCTTAGG TAGCTCAGGC TCAGGGAAAA CCACGCTGCT GGACGCCATC 
TCTGGGAGGC TGCGGCGCAC AGGGACCTTG GAAGGGGAAG TGTTTGTGAA CGGCTGCGAG 
CTGCGCAGGG ACCAGTTCCA AGACTGCGTC TCCTACCTCC TGCAGAGCGA TGTCTTTCTG 
AGCAGCCTCA CGGTGCGGGA GACGCTGAGA TACACGGCGA TGCTGGCTCT CCGCAGCAGC 
TCCGCGGACT TCTACGACAA GAAGGTAGAG GCAGTCCTGA CAGAGCTGAG TCTGAGCCAC 
GTGGCAGACC AAATGATCGG CAACTATAAT TTTGGGGGGA TTTCCAGTGG CGAGCGGCGC 
CGAGTGTCCA TCGCAGCCCA ACTCCTTCAG GACCCCAAGG TCATGATGCT TGACGAGCCA 
ACCACAGGAC TGGACTGCAT GACTGCAAAT CATATCGTCC TCCTCTTGGT CGAGCTGGCT 
CGCAGGAACC GCATTGTAAT TGTCACCATC CACCAGCCTC GCTCTGAGCT CTTCCACCAC 
TTCGACAAAA TTGCCATTCT GACTTACGGA GAGTTGGTGT TCTGTGGCAC GCCAGAGGAG 
ATGCTCGGCT TCTTCAATAA CTGTGGTTAC CCCTGTCCTG AACATTCCAA TCCCTTTGAT 
TTCTACATGG ACTTGACATC GGTGGACACC CAAAGCAGAG AGCGAGAGAT AGAGACGTAC 
AAGCGAGTCC AGATGCTGGA ATCTGCCTTC AGGCAATCGG ACATCTGTCA CAAAATCCTG 
GAGAACATTG AAAGAACAAG ACACCTGAAA ACCCTACCCA TGGTTCCTTT CAAAACGAAA 
AATCCTCCCG GAATGTTCTG CAAGCTCGGC GTTCTCCTGA GGAGAGTAAC GAGAAACCTA 
ATGAGGAATA AGCAGGTGGT GATTATGCGT CTTGTTCAGA ATCTGATCAT GGGTCTGTTC 
CTCATTTTCT ACCTTCTCCG AGTCCAGAAC AACATGCTGA AGGGCGCTGT TCAGGACCGC 
GTAGGGCTGT TGTACCAGCT TGTGGGTGCC ACCCCGTACA CCGGCATGCT CAACGCTGTG 
AACCTCTTTC CCATGCTGAG AGCTGTCAGC GACCAGGAGA GTCAGGATGG CCTGTACCAG 
AAGTGGCAGA TGCTGCTCGC CTATGTGCTG CATGCTCTCC CCTTCAGCAT CGTTGCCACG 
GTGATTTTCA GCAGCGTGTG TTACTGGACT CTGGGCTTGT ATCCCGAGGT CGCCAGATTT 
GGATACTTCT CTGCCGCTCT GTTGGCCCCT CACTTAATTG GAGAATTTCT GACACTTGTG 
CTGCTTGGTA TGGTCCAAAA CCCCAATATT GTCAACAGCA TAGTGGCTCT GCTGAGTATT 
TCTGGGTTGC TCATTGGATC TGGATTTATC AGAAACATAG AAGAAATGCC CATTCCTTTA 
AAAATCCTGG GTTACTTTAC CTTCCAAAAG TACTGTTGTG AGATTCTTGT GGTCAATGAG 
TTCTATGGCC TGAACTTCAC TTGTGGTGGC TCCAACACTT CTGTGCCAAA TAACCCAATG 
TGTTCCATGA CCCAAGGGAT CCAATTCATT GAGAAAACCT GCCCAGGGGC CACGTCCAGA 
TTCACGACAA ACTTCCTGAT CTTGTACTCG TTCATCCCGA CTCTTGTCAT CCTGGGGATG 
GTGGTCTTTA AAGTCCGGGA CTACCTGATT AGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446206.2
CDS	65..2023
Translation	MSELPFLSPEGARGPHNNRGSQSSLEEGSVTGSEARHSLGVLNVSFSVSNRVGPWWNIKSCQQKWDRKILKDVSLYIESGQTMCILGSSGSGKTTLLDAISGRLRRTGTLEGEVFVNGCELRRDQFQDCVSYLLQSDVFLSSLTVRETLRYTAMLALRSSSADFYDKKVEAVLTELSLSHVADQMIGNYNFGGISSGERRRVSIAAQLLQDPKVMMLDEPTTGLDCMTANHIVLLLVELARRNRIVIVTIHQPRSELFHHFDKIAILTYGELVFCGTPEEMLGFFNNCGYPCPEHSNPFDFYMDLTSVDTQSREREIETYKRVQMLESAFRQSDICHKILENIERTRHLKTLPMVPFKTKNPPGMFCKLGVLLRRVTRNLMRNKQVVIMRLVQNLIMGLFLIFYLLRVQNNMLKGAVQDRVGLLYQLVGATPYTGMLNAVNLFPMLRAVSDQESQDGLYQKWQMLLAYVLHALPFSIVATVIFSSVCYWTLGLYPEVARFGYFSAALLAPHLIGEFLTLVLLGMVQNPNIVNSIVALLSISGLLIGSGFIRNIEEMPIPLKILGYFTFQKYCCEILVVNEFYGLNFTCGGSNTSVPNNPMCSMTQGIQFIEKTCPGATSRFTTNFLILYSFIPTLVILGMVVFKVRDYLISR

Target ORF information:

RefSeq Version	NM_053754.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily G member 5 (Abcg5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053754.2

ORF Insert Sequence:

ATGAGTGAGC TGCCCTTTCT GAGTCCAGAG GGAGCCAGAG GGCCTCACAA CAACAGAGGG 
TCTCAGAGCT CCCTGGAGGA AGGCTCAGTT ACAGGCTCAG AGGCTCGGCA CAGCTTAGGT 
GTCCTGAATG TGTCCTTCAG CGTCAGCAAC CGTGTCGGGC CCTGGTGGAA CATCAAATCA 
TGCCAGCAGA AGTGGGACAG GAAAATCCTC AAAGATGTCT CCTTGTACAT CGAGAGTGGC 
CAGACCATGT GCATCTTAGG TAGCTCAGGC TCAGGGAAAA CCACGCTGCT GGACGCCATC 
TCTGGGAGGC TGCGGCGCAC AGGGACCTTG GAAGGGGAAG TGTTTGTGAA CGGCTGCGAG 
CTGCGCAGGG ACCAGTTCCA AGACTGCGTC TCCTACCTCC TGCAGAGCGA TGTCTTTCTG 
AGCAGCCTCA CGGTGCGGGA GACGCTGAGA TACACGGCGA TGCTGGCTCT CCGCAGCAGC 
TCCGCGGACT TCTACGACAA GAAGGTAGAG GCAGTCCTGA CAGAGCTGAG TCTGAGCCAC 
GTGGCAGACC AAATGATCGG CAACTATAAT TTTGGGGGGA TTTCCAGTGG CGAGCGGCGC 
CGAGTGTCCA TCGCAGCCCA ACTCCTTCAG GACCCCAAGG TCATGATGCT TGACGAGCCA 
ACCACAGGAC TGGACTGCAT GACTGCAAAT CATATCGTCC TCCTCTTGGT CGAGCTGGCT 
CGCAGGAACC GCATTGTAAT TGTCACCATC CACCAGCCTC GCTCTGAGCT CTTCCACCAC 
TTCGACAAAA TTGCCATTCT GACTTACGGA GAGTTGGTGT TCTGTGGCAC GCCAGAGGAG 
ATGCTCGGCT TCTTCAATAA CTGTGGTTAC CCCTGTCCTG AACATTCCAA TCCCTTTGAT 
TTCTACATGG ACTTGACATC GGTGGACACC CAAAGCAGAG AGCGAGAGAT AGAGACGTAC 
AAGCGAGTCC AGATGCTGGA ATCTGCCTTC AGGCAATCGG ACATCTGTCA CAAAATCCTG 
GAGAACATTG AAAGAACAAG ACACCTGAAA ACCCTACCCA TGGTTCCTTT CAAAACGAAA 
AATCCTCCCG GAATGTTCTG CAAGCTCGGC GTTCTCCTGA GGAGAGTAAC GAGAAACCTA 
ATGAGGAATA AGCAGGTGGT GATTATGCGT CTTGTTCAGA ATCTGATCAT GGGTCTGTTC 
CTCATTTTCT ACCTTCTCCG AGTCCAGAAC AACATGCTGA AGGGCGCTGT TCAGGACCGC 
GTAGGGCTGT TGTACCAGCT TGTGGGTGCC ACCCCGTACA CCGGCATGCT CAACGCTGTG 
AACCTCTTTC CCATGCTGAG AGCTGTCAGC GACCAGGAGA GTCAGGATGG CCTGTACCAG 
AAGTGGCAGA TGCTGCTCGC CTATGTGCTG CATGCTCTCC CCTTCAGCAT CGTTGCCACG 
GTGATTTTCA GCAGCGTGTG TTACTGGACT CTGGGCTTGT ATCCCGAGGT CGCCAGATTT 
GGATACTTCT CTGCCGCTCT GTTGGCCCCT CACTTAATTG GAGAATTTCT GACACTTGTG 
CTGCTTGGTA TGGTCCAAAA CCCCAATATT GTCAACAGCA TAGTGGCTCT GCTGAGTATT 
TCTGGGTTGC TCATTGGATC TGGATTTATC AGAAACATAG AAGAAATGCC CATTCCTTTA 
AAAATCCTGG GTTACTTTAC CTTCCAAAAG TACTGTTGTG AGATTCTTGT GGTCAATGAG 
TTCTATGGCC TGAACTTCAC TTGTGGTGGC TCCAACACTT CTGTGCCAAA TAACCCAATG 
TGTTCCATGA CCCAAGGGAT CCAATTCATT GAGAAAACCT GCCCAGGGGC CACGTCCAGA 
TTCACGACAA ACTTCCTGAT CTTGTACTCG TTCATCCCGA CTCTTGTCAT CCTGGGGATG 
GTGGTCTTTA AAGTCCGGGA CTACCTGATT AGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcg5 ( XM_017593976.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449465.1 Rattus norvegicus ATP binding cassette subfamily G member 5 (Abcg5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61510
Clone ID Related Accession (Same CDS sequence)	XM_017593976.1
Accession Version	XM_017593976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-binding cassette sub-family G member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGTA TGTGGAAAGT CAGAGGACAA CATCTGGTAA TTCAGTCTCT CCCTCCACCT 
CTAGGACTGA GCTCGGGTCC TCAGGCTTGG CTGCAAGCAC GCTTACTCTC TGAAGAGCAC 
ATACAGGGGT TCTTCAGACT TGGATCCCAG GGCCTTCTCT TGTCGCCCCT AGGCTCAGGG 
AAAACCACGC TGCTGGACGC CATCTCTGGG AGGCTGCGGC GCACAGGGAC CTTGGAAGGG 
GAAGTGTTTG TGAACGGCTG CGAGCTGCGC AGGGACCAGT TCCAAGACTG CGTCTCCTAC 
CTCCTGCAGA GCGATGTCTT TCTGAGCAGC CTCACGGTGC GGGAGACGCT GAGATACACG 
GCGATGCTGG CTCTCCGCAG CAGCTCCGCG GACTTCTACG ACAAGAAGGT AGAGGCAGTC 
CTGACAGAGC TGAGTCTGAG CCACGTGGCA GACCAAATGA TCGGCAACTA TAATTTTGGG 
GGGATTTCCA GTGGCGAGCG GCGCCGAGTG TCCATCGCAG CCCAACTCCT TCAGGACCCC 
AAGGTCATGA TGCTTGACGA GCCAACCACA GGACTGGACT GCATGACTGC AAATCATATC 
GTCCTCCTCT TGGTCGAGCT GGCTCGCAGG AACCGCATTG TAATCGTCAC CATCCACCAG 
CCTCGCTCTG AGCTCTTCCA CCACTTCGAC AAAATTGCCA TTCTGACTTA CGGAGAGTTG 
GTGTTCTGTG GCACGCCAGA GGAGATGCTC GGCTTCTTCA ATAACTGTGG TTACCCCTGT 
CCTGAACATT CCAATCCCTT TGATTTCTAC ATGGACTTGA CATCGGTGGA CACCCAAAGC 
AGAGAGCGAG AGATAGAGAC GTACAAGCGA GTCCAGATGC TGGAATCTGC CTTCAGGCAA 
TCGGACATCT GTCACAAAAT CCTGGAGAAC ATTGAAAGAA CAAGACACCT GAAAACCTTA 
CCCATGGTTC CTTTCAAAAC GAAAAATCCT CCCGGAATGT TCTGCAAGCT CGGCGTTCTC 
CTGAGGAGAG TAACGAGAAA CCTAATGAGG AATAAGCAGG TGGTGATTAT GCGTCTTGTT 
CAGAATCTGA TCATGGGTCT GTTCCTCATT TTCTACCTTC TCCGAGTCCA GAACAACATG 
CTGAAGGGCG CTGTTCAGGA CCGTGTAGGG CTGTTGTACC AGCTCGTGGG TGCCACCCCG 
TACACCGGCA TGCTCAACGC TGTGAACCTC TTTCCCATGC TGAGAGCTGT CAGCGACCAG 
GAGAGTCAGG ATGGCCTGTA CCAGAAGTGG CAGATGCTGC TCGCCTATGT GCTGCATGCT 
CTCCCCTTCA GCATCGTTGC CACGGTGATT TTCAGCAGCG TGTGTTACTG GACTCTGGGC 
TTGTATCCCG AGGTCGCCAG ATTTGGATAC TTCTCTGCCG CTCTGTTGGC CCCTCACTTA 
ATTGGAGAAT TTCTGACACT TGTGCTGCTT GGTATGGTCC AAAACCCCAA TATTGTCAAC 
AGCATAGTGG CTCTGCTGAG TATTTCTGGG TTGCTCATTG GATCTGGATT TATCAGAAAC 
ATAGAAGAAA TGCCCATTCC TTTAAAAATC CTGGGTTACT TTACCTTCCA AAAGTACTGT 
TGTGAGATTC TTGTGGTCAA TGAGTTCTAT GGCCTGAACT TCACTTGTGG TGGCTCCAAC 
ACTTCTGTGC CAAATAACCC AATGTGTTCC ATGACCCAAG GGATCCAATT CATTGAGAAA 
ACCTGCCCAG GGGCCACGTC CAGATTCACG ACAAACTTCC TGATCTTGTA CTCGTTCATC 
CCGACTCTTG TCATCCTGGG GATGGTGGTC TTTAAAGTCC GGGACTACCT GATTAGCAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449465.1
CDS	120..1982
Translation	MACMWKVRGQHLVIQSLPPPLGLSSGPQAWLQARLLSEEHIQGFFRLGSQGLLLSPLGSGKTTLLDAISGRLRRTGTLEGEVFVNGCELRRDQFQDCVSYLLQSDVFLSSLTVRETLRYTAMLALRSSSADFYDKKVEAVLTELSLSHVADQMIGNYNFGGISSGERRRVSIAAQLLQDPKVMMLDEPTTGLDCMTANHIVLLLVELARRNRIVIVTIHQPRSELFHHFDKIAILTYGELVFCGTPEEMLGFFNNCGYPCPEHSNPFDFYMDLTSVDTQSREREIETYKRVQMLESAFRQSDICHKILENIERTRHLKTLPMVPFKTKNPPGMFCKLGVLLRRVTRNLMRNKQVVIMRLVQNLIMGLFLIFYLLRVQNNMLKGAVQDRVGLLYQLVGATPYTGMLNAVNLFPMLRAVSDQESQDGLYQKWQMLLAYVLHALPFSIVATVIFSSVCYWTLGLYPEVARFGYFSAALLAPHLIGEFLTLVLLGMVQNPNIVNSIVALLSISGLLIGSGFIRNIEEMPIPLKILGYFTFQKYCCEILVVNEFYGLNFTCGGSNTSVPNNPMCSMTQGIQFIEKTCPGATSRFTTNFLILYSFIPTLVILGMVVFKVRDYLISR

Target ORF information:

RefSeq Version	XM_017593976.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily G member 5 (Abcg5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593976.1

ORF Insert Sequence:

ATGGCATGTA TGTGGAAAGT CAGAGGACAA CATCTGGTAA TTCAGTCTCT CCCTCCACCT 
CTAGGACTGA GCTCGGGTCC TCAGGCTTGG CTGCAAGCAC GCTTACTCTC TGAAGAGCAC 
ATACAGGGGT TCTTCAGACT TGGATCCCAG GGCCTTCTCT TGTCGCCCCT AGGCTCAGGG 
AAAACCACGC TGCTGGACGC CATCTCTGGG AGGCTGCGGC GCACAGGGAC CTTGGAAGGG 
GAAGTGTTTG TGAACGGCTG CGAGCTGCGC AGGGACCAGT TCCAAGACTG CGTCTCCTAC 
CTCCTGCAGA GCGATGTCTT TCTGAGCAGC CTCACGGTGC GGGAGACGCT GAGATACACG 
GCGATGCTGG CTCTCCGCAG CAGCTCCGCG GACTTCTACG ACAAGAAGGT AGAGGCAGTC 
CTGACAGAGC TGAGTCTGAG CCACGTGGCA GACCAAATGA TCGGCAACTA TAATTTTGGG 
GGGATTTCCA GTGGCGAGCG GCGCCGAGTG TCCATCGCAG CCCAACTCCT TCAGGACCCC 
AAGGTCATGA TGCTTGACGA GCCAACCACA GGACTGGACT GCATGACTGC AAATCATATC 
GTCCTCCTCT TGGTCGAGCT GGCTCGCAGG AACCGCATTG TAATCGTCAC CATCCACCAG 
CCTCGCTCTG AGCTCTTCCA CCACTTCGAC AAAATTGCCA TTCTGACTTA CGGAGAGTTG 
GTGTTCTGTG GCACGCCAGA GGAGATGCTC GGCTTCTTCA ATAACTGTGG TTACCCCTGT 
CCTGAACATT CCAATCCCTT TGATTTCTAC ATGGACTTGA CATCGGTGGA CACCCAAAGC 
AGAGAGCGAG AGATAGAGAC GTACAAGCGA GTCCAGATGC TGGAATCTGC CTTCAGGCAA 
TCGGACATCT GTCACAAAAT CCTGGAGAAC ATTGAAAGAA CAAGACACCT GAAAACCTTA 
CCCATGGTTC CTTTCAAAAC GAAAAATCCT CCCGGAATGT TCTGCAAGCT CGGCGTTCTC 
CTGAGGAGAG TAACGAGAAA CCTAATGAGG AATAAGCAGG TGGTGATTAT GCGTCTTGTT 
CAGAATCTGA TCATGGGTCT GTTCCTCATT TTCTACCTTC TCCGAGTCCA GAACAACATG 
CTGAAGGGCG CTGTTCAGGA CCGTGTAGGG CTGTTGTACC AGCTCGTGGG TGCCACCCCG 
TACACCGGCA TGCTCAACGC TGTGAACCTC TTTCCCATGC TGAGAGCTGT CAGCGACCAG 
GAGAGTCAGG ATGGCCTGTA CCAGAAGTGG CAGATGCTGC TCGCCTATGT GCTGCATGCT 
CTCCCCTTCA GCATCGTTGC CACGGTGATT TTCAGCAGCG TGTGTTACTG GACTCTGGGC 
TTGTATCCCG AGGTCGCCAG ATTTGGATAC TTCTCTGCCG CTCTGTTGGC CCCTCACTTA 
ATTGGAGAAT TTCTGACACT TGTGCTGCTT GGTATGGTCC AAAACCCCAA TATTGTCAAC 
AGCATAGTGG CTCTGCTGAG TATTTCTGGG TTGCTCATTG GATCTGGATT TATCAGAAAC 
ATAGAAGAAA TGCCCATTCC TTTAAAAATC CTGGGTTACT TTACCTTCCA AAAGTACTGT 
TGTGAGATTC TTGTGGTCAA TGAGTTCTAT GGCCTGAACT TCACTTGTGG TGGCTCCAAC 
ACTTCTGTGC CAAATAACCC AATGTGTTCC ATGACCCAAG GGATCCAATT CATTGAGAAA 
ACCTGCCCAG GGGCCACGTC CAGATTCACG ACAAACTTCC TGATCTTGTA CTCGTTCATC 
CCGACTCTTG TCATCCTGGG GATGGTGGTC TTTAAAGTCC GGGACTACCT GATTAGCAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic Control of Serum Marinobufagenin in the Spontaneously Hypertensive Rat and the Relationship to Blood Pressure.
Journal of the American Heart Association6(10)(2017 Oct)
Dmitrieva RI,Cranford SM,Doris PA

Crystal structure of the human sterol transporter ABCG5/ABCG8.
Nature533(7604)561-4(2016 05)
Lee JY,Kinch LN,Borek DM,Wang J,Wang J,Urbatsch IL,Xie XS,Grishin NV,Cohen JC,Otwinowski Z,Hobbs HH,Rosenbaum DM

Novel candidate genes for impaired nephron development in a rat model with inherited nephron deficit and albuminuria.
Clinical and experimental pharmacology & physiology42(10)1051-8(2015 Oct)
Herlan L,Schulz A,Schulte L,Schulz H,H?bner N,Kreutz R

Influence of dietary sugar on cholesterol and bile acid metabolism in?the rat: Marked reduction of hepatic Abcg5/8 expression following sucrose ingestion.
Biochemical and biophysical research communications461(4)592-7(2015 Jun)
Apro J,Beckman L,Angelin B,Rudling M

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