Wif1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wif1
Entrez Gene ID	114557
Full Name	Wnt inhibitory factor 1
General protein information	Preferred Names Wnt inhibitory factor 1 Names wnt inhibitory factor 1 WIF-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22
Summary	human homolog has decreased mRNA expression in some prostate cancers and other cancers; may act as a secreted inhibitor of the Wnt signaling pathway [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053738.1	NP_446190.1	wnt inhibitory factor 1 precursor
XM_006241395.3	XP_006241457.1	wnt inhibitory factor 1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	WIF1	NP_001186536.1
Rattus norvegicus (Norway rat)	Wif1	NP_446190.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000117	XP_311028.4
Danio rerio (zebrafish)	wif1	NP_571304.1
Homo sapiens (human)	WIF1	NP_009122.2
Pan troglodytes (chimpanzee)	WIF1	XP_001163469.1
	WIF1	XP_001116962.1
Canis lupus familiaris (dog)	WIF1	XP_538269.2
Mus musculus (house mouse)	Wif1	NP_036045.1
Drosophila melanogaster (fruit fly)	shf	NP_572349.1
Xenopus tropicalis (tropical clawed frog)	wif1	NP_001106386.1

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-17-5p-activated Wnt/?-catenin pathway contributes to the progression of liver fibrosis.
Yu F, Lu Z, Huang K, Wang X, Xu Z, Chen B, Dong P, Zheng J
Oncotarget7(1)81-93(2016 Jan)

Smad1 and WIF1 genes are downregulated during saccular stage of lung development in the nitrofen rat model.
Fujiwara N, Doi T, Gosemann JH, Kutasy B, Friedmacher F, Puri P
Pediatric surgery international28(2)189-93(2012 Feb)

Role of Wnt inhibitory factor-1 and Wnt/wingless signaling in choroid plexus tumors.
Tomm M, Koch A, Mertsch S, Wrede B, Jeibmann A, Wolff J, Paulus W, Hasselblatt M
Pediatric blood & cancer53(6)1152-5(2009 Dec)

A comparison of the performance of microsatellite and methylation urine analysis for predicting the recurrence of urothelial cell carcinoma, and definition of a set of markers by Bayesian network analysis.
Roupr?t M, Hupertan V, Yates DR, Comperat E, Catto JW, Meuth M, Lackmichi A, Ricci S, Lacave R, Gattegno B, Richard F, Hamdy FC, Cussenot O
BJU international101(11)1448-53(2008 Jun)

Wnt signalling and its impact on development and cancer.
Klaus A, Birchmeier W
Nature reviews. Cancer8(5)387-98(2008 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that microRNA miR-17-5p over-expression promoted the activation of hepatic stellate cell (HSC) and contributed to the reduction of Wnt inhibitory factor 1 (WIF1) levels.
Title: MicroRNA-17-5p-activated Wnt/?-catenin pathway contributes to the progression of liver fibrosis.

downregulation of Smad1/Wif1 gene expression may contribute to pulmonary hypoplasia in the nitrofen CDH model by retardation of lung development during saccular stage.
Title: Smad1 and WIF1 genes are downregulated during saccular stage of lung development in the nitrofen rat model.

Wif-1 inhibits proliferation of neoplastic choroid plexus epithelial cells
Title: Role of Wnt inhibitory factor-1 and Wnt/wingless signaling in choroid plexus tumors.

GLT mRNA was translated in Wnt-1-expressing C6 cells when the GLT mRNA required no splicing before translation occurred
Title: Wnt-1 has multiple effects on the expression of glutamate transporters.

The following Wif1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wif1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10476	NM_053738.1 Latest version!	Rattus norvegicus Wnt inhibitory factor 1 (Wif1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa30154	XM_006241395.3 Latest version!	Rattus norvegicus Wnt inhibitory factor 1 (Wif1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wif1 ( NM_053738.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446190.1 Rattus norvegicus Wnt inhibitory factor 1 (Wif1), mRNA.

Price & Availability↑

CloneID	ORa10476
Clone ID Related Accession (Same CDS sequence)	NM_053738.1
Accession Version	NM_053738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	wnt inhibitory factor 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY030278.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY030278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642800 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCGGA GAAGAGCCTT CCCTGCTTTC GTGCTCCGGC TCTGGAGCAT CCTACCTTGC 
CTGCTCCTGC TACGAGCGGA TGCAGGGCAG CCGCCAGAGG AGAGCTTGTA CCTGTGGATC 
GACGCCCATC AGGCCAGAGT ACTCATAGGA TTTGAAGAAG ATATTCTGAT TGTCTCGGAG 
GGGAAAATGG CCCCCTTTAC ACATGATTTC AGGAAAGCCC AACAAAGAAT GCCAGCCATT 
CCCGTCAATA TCCACTCCAT GAATTTTACC TGGCAAGCTT CAGGGCAGGC AGAGTACTTC 
TATGAGTTCC TGTCGCTGCG CTCGCTGGAT AAAGGCATCA TGGCAGACCC AACTGTCAAT 
GTCCCTCGGC TGGGAACAGT GCCTCACAAG GCATCAGTTG TTCAAGTTGG TTTCCCGTGT 
CTCGGCAAAC AGGATGGGGT GGCAGCATTT GAAGTGAATG TGATTGTCAT GAATTCTGAA 
GGCAACCCCA TCCTTCGGAC CCCTCAAAAT GCTATCTTCT TTAAAACATG TCAACAAGCT 
GAGTGCCCAG GAGGGTGTCG AAATGGAGGC TTTTGTAACG AAAGGCGGGT CTGCGAGTGT 
CCCGATGGGT TCTATGGACC TCACTGTGAG AAAGCCCTCT GCATACCTCG ATGTATGAAC 
GGTGGTCTGT GTGTCACTCC TGGCTTCTGC ATCTGCCCGC CTGGATTCTA CGGTGTCAAC 
TGTGACAAAG CAAACTGCTC GGCCACCTGC TTTAATGGAG GGACCTGTTT TTACCCAGGA 
AAATGTATTT GCCCTCCAGG ACTTGAGGGA GAGCAGTGTG AACTCAGCAA GTGCCCCCAA 
CCCTGCCGAA ACGGAGGTAA ATGCATTGGT AAAAGCAAGT CTGTCTGCGA GCCTGGCTGC 
GGTGCCCATG GAACCTGCCA CGAACCCAAC AAATGCCAGT GTCGAGAGGG CTGGCATGGG 
AGACACTGCA ATAAAAGGTA CGGAGCCAGC CTCATGCATG CCCCGAGGCC AGCAGGCGCC 
GGGCTGGAGC GGCACACGCC TTCACTTAAA AAGGCTGAGG GGCGGAGGGA TCCACCTGAA 
TCCAATTACA TCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446190.1
CDS	315..1412
Translation	MARRRAFPAFVLRLWSILPCLLLLRADAGQPPEESLYLWIDAHQARVLIGFEEDILIVSEGKMAPFTHDFRKAQQRMPAIPVNIHSMNFTWQASGQAEYFYEFLSLRSLDKGIMADPTVNVPRLGTVPHKASVVQVGFPCLGKQDGVAAFEVNVIVMNSEGNPILRTPQNAIFFKTCQQAECPGGCRNGGFCNERRVCECPDGFYGPHCEKALCIPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSATCFNGGTCFYPGKCICPPGLEGEQCELSKCPQPCRNGGKCIGKSKSVCEPGCGAHGTCHEPNKCQCREGWHGRHCNKRYGASLMHAPRPAGAGLERHTPSLKKAEGRRDPPESNYIW

Target ORF information:

RefSeq Version	NM_053738.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Wnt inhibitory factor 1 (Wif1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053738.1

ORF Insert Sequence:

ATGGCCCGGA GAAGAGCCTT CCCTGCTTTC GTGCTCCGGC TCTGGAGCAT CCTACCTTGC 
CTGCTCCTGC TACGAGCGGA TGCAGGGCAG CCGCCAGAGG AGAGCTTGTA CCTGTGGATC 
GACGCCCATC AGGCCAGAGT ACTCATAGGA TTTGAAGAAG ATATTCTGAT TGTCTCGGAG 
GGGAAAATGG CCCCCTTTAC ACATGATTTC AGGAAAGCCC AACAAAGAAT GCCAGCCATT 
CCCGTCAATA TCCACTCCAT GAATTTTACC TGGCAAGCTT CAGGGCAGGC AGAGTACTTC 
TATGAGTTCC TGTCGCTGCG CTCGCTGGAT AAAGGCATCA TGGCAGACCC AACTGTCAAT 
GTCCCTCGGC TGGGAACAGT GCCTCACAAG GCATCAGTTG TTCAAGTTGG TTTCCCGTGT 
CTCGGCAAAC AGGATGGGGT GGCAGCATTT GAAGTGAATG TGATTGTCAT GAATTCTGAA 
GGCAACCCCA TCCTTCGGAC CCCTCAAAAT GCTATCTTCT TTAAAACATG TCAACAAGCT 
GAGTGCCCAG GAGGGTGTCG AAATGGAGGC TTTTGTAACG AAAGGCGGGT CTGCGAGTGT 
CCCGATGGGT TCTATGGACC TCACTGTGAG AAAGCCCTCT GCATACCTCG ATGTATGAAC 
GGTGGTCTGT GTGTCACTCC TGGCTTCTGC ATCTGCCCGC CTGGATTCTA CGGTGTCAAC 
TGTGACAAAG CAAACTGCTC GGCCACCTGC TTTAATGGAG GGACCTGTTT TTACCCAGGA 
AAATGTATTT GCCCTCCAGG ACTTGAGGGA GAGCAGTGTG AACTCAGCAA GTGCCCCCAA 
CCCTGCCGAA ACGGAGGTAA ATGCATTGGT AAAAGCAAGT CTGTCTGCGA GCCTGGCTGC 
GGTGCCCATG GAACCTGCCA CGAACCCAAC AAATGCCAGT GTCGAGAGGG CTGGCATGGG 
AGACACTGCA ATAAAAGGTA CGGAGCCAGC CTCATGCATG CCCCGAGGCC AGCAGGCGCC 
GGGCTGGAGC GGCACACGCC TTCACTTAAA AAGGCTGAGG GGCGGAGGGA TCCACCTGAA 
TCCAATTACA TCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wif1 ( XM_006241395.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241457.1 Rattus norvegicus Wnt inhibitory factor 1 (Wif1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30154
Clone ID Related Accession (Same CDS sequence)	XM_006241395.3
Accession Version	XM_006241395.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	wnt inhibitory factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241395.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGA GAAGAGCCTT CCCTGCTTTC GTGCTCCGGC TCTGGAGCAT CCTACCTTGC 
CTGCTCCTGC TACGAGCGGA TGCAGGGCAG CCGCCAGAGG AGAGCTTGTA CCTGTGGATC 
GACGCCCATC AGGCCAGAGT ACTCATAGGA TTTGAAGAAG ATATTCTGAT TGTCTCGGAG 
GGGAAAATGG CCCCCTTTAC ACATGATTTC AGGAAAGCCC AACAAAGAAT GCCAGCCATT 
CCCGTCAATA TCCACTCCAT GAATTTTACC TGGCAAGCTT CAGGGCAGGC AGAGTACTTC 
TATGAGTTCC TGTCGCTGCG CTCGCTGGAT AAAGGCATCA TGGCAGACCC AACTGTCAAT 
GTCCCTCGGC TGGGAACAGT GCCTCACAAG GCATCAGTTG TTCAAGTTGG TTTCCCGTGT 
CTCGGCAAAC AGGATGGGGT GGCAGCATTT GAAGTGAATG TGATTGTCAT GAATTCTGAA 
GGCAACCCCA TCCTTCGGAC CCCTCAAAAT GCTATCTTCT TTAAAACATG TCAACAAGCT 
GAGTGCCCAG GAGGGTGTCG AAATGGAGGC TTTTGTAACG AAAGGCGGGT CTGCGAGTGT 
CCCGATGGGT TCTATGGACC TCACTGTGAG AAAGCCCTCT GCATACCTCG ATGTATGAAC 
GGTGGTCTGT GTGTCACTCC TGGCTTCTGC ATCTGCCCGC CTGGATTCTA CGGTGTCAAC 
TGTGACAAAG CAAACTGCTC GGCCACCTGC TTTAATGGAG GGACCTGTTT TTACCCAGGA 
AAATGTATTT GCCCTCCAGG ACTTGAGGGA GAGCAGTGTG AACTCAGCAA GTGCCCCCAA 
CCCTGCCGAA ACGGAGGTAA ATGCATTGGT AAAAGCAAGT GTAAGTGTCC GAAAGGTTAC 
CAAGGAGACC TGTGCTCTAA GCCTGTCTGC GAGCCTGGCT GCGGTGCCCA TGGAACCTGC 
CACGAACCCA ACAAATGCCA GTGTCGAGAG GGCTGGCATG GGAGACACTG CAATAAAAGG 
TACGGAGCCA GCCTCATGCA TGCCCCGAGG CCAGCAGGCG CCGGGCTGGA GCGGCACACG 
CCTTCACTTA AAAAGGCTGA GGGGCGGAGG GATCCACCTG AATCCAATTA CATCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241457.1
CDS	307..1446
Translation	MARRRAFPAFVLRLWSILPCLLLLRADAGQPPEESLYLWIDAHQARVLIGFEEDILIVSEGKMAPFTHDFRKAQQRMPAIPVNIHSMNFTWQASGQAEYFYEFLSLRSLDKGIMADPTVNVPRLGTVPHKASVVQVGFPCLGKQDGVAAFEVNVIVMNSEGNPILRTPQNAIFFKTCQQAECPGGCRNGGFCNERRVCECPDGFYGPHCEKALCIPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSATCFNGGTCFYPGKCICPPGLEGEQCELSKCPQPCRNGGKCIGKSKCKCPKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCREGWHGRHCNKRYGASLMHAPRPAGAGLERHTPSLKKAEGRRDPPESNYIW

Target ORF information:

RefSeq Version	XM_006241395.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Wnt inhibitory factor 1 (Wif1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241395.3

ORF Insert Sequence:

ATGGCCCGGA GAAGAGCCTT CCCTGCTTTC GTGCTCCGGC TCTGGAGCAT CCTACCTTGC 
CTGCTCCTGC TACGAGCGGA TGCAGGGCAG CCGCCAGAGG AGAGCTTGTA CCTGTGGATC 
GACGCCCATC AGGCCAGAGT ACTCATAGGA TTTGAAGAAG ATATTCTGAT TGTCTCGGAG 
GGGAAAATGG CCCCCTTTAC ACATGATTTC AGGAAAGCCC AACAAAGAAT GCCAGCCATT 
CCCGTCAATA TCCACTCCAT GAATTTTACC TGGCAAGCTT CAGGGCAGGC AGAGTACTTC 
TATGAGTTCC TGTCGCTGCG CTCGCTGGAT AAAGGCATCA TGGCAGACCC AACTGTCAAT 
GTCCCTCGGC TGGGAACAGT GCCTCACAAG GCATCAGTTG TTCAAGTTGG TTTCCCGTGT 
CTCGGCAAAC AGGATGGGGT GGCAGCATTT GAAGTGAATG TGATTGTCAT GAATTCTGAA 
GGCAACCCCA TCCTTCGGAC CCCTCAAAAT GCTATCTTCT TTAAAACATG TCAACAAGCT 
GAGTGCCCAG GAGGGTGTCG AAATGGAGGC TTTTGTAACG AAAGGCGGGT CTGCGAGTGT 
CCCGATGGGT TCTATGGACC TCACTGTGAG AAAGCCCTCT GCATACCTCG ATGTATGAAC 
GGTGGTCTGT GTGTCACTCC TGGCTTCTGC ATCTGCCCGC CTGGATTCTA CGGTGTCAAC 
TGTGACAAAG CAAACTGCTC GGCCACCTGC TTTAATGGAG GGACCTGTTT TTACCCAGGA 
AAATGTATTT GCCCTCCAGG ACTTGAGGGA GAGCAGTGTG AACTCAGCAA GTGCCCCCAA 
CCCTGCCGAA ACGGAGGTAA ATGCATTGGT AAAAGCAAGT GTAAGTGTCC GAAAGGTTAC 
CAAGGAGACC TGTGCTCTAA GCCTGTCTGC GAGCCTGGCT GCGGTGCCCA TGGAACCTGC 
CACGAACCCA ACAAATGCCA GTGTCGAGAG GGCTGGCATG GGAGACACTG CAATAAAAGG 
TACGGAGCCA GCCTCATGCA TGCCCCGAGG CCAGCAGGCG CCGGGCTGGA GCGGCACACG 
CCTTCACTTA AAAAGGCTGA GGGGCGGAGG GATCCACCTG AATCCAATTA CATCTGGTGA