Emb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Emb
Entrez Gene ID	114511
Full Name	embigin
General protein information	Preferred Names embigin Names embigin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q15
Summary	a cell adhesion molecule; may function as a regulator of cell/extracellular matrix interactions during development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053719.1	NP_446171.1	embigin precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	EMB	NP_989888.1
Homo sapiens (human)	EMB	NP_940851.1
Pan troglodytes (chimpanzee)	EMB	XP_001151688.1
Xenopus tropicalis (tropical clawed frog)	emb	XP_002933091.2
Danio rerio (zebrafish)	si:ch211-261c8.5	NP_001108583.1
	EMB	XP_001093157.1
Rattus norvegicus (Norway rat)	Emb	NP_446171.1
Canis lupus familiaris (dog)	EMB	XP_005619515.1
Bos taurus (cattle)	EMB	XP_005221593.1
Mus musculus (house mouse)	Emb	NP_034460.3

Gene Ontology
Bibliography

Related articles in PubMed

The inhibition of monocarboxylate transporter 2 (MCT2) by AR-C155858 is modulated by the associated ancillary protein.
Ovens MJ, Manoharan C, Wilson MC, Murray CM, Halestrap AP
The Biochemical journal431(2)217-25(2010 Oct)

Studies on the DIDS-binding site of monocarboxylate transporter 1 suggest a homology model of the open conformation and a plausible translocation cycle.
Wilson MC, Meredith D, Bunnun C, Sessions RB, Halestrap AP
The Journal of biological chemistry284(30)20011-21(2009 Jul)

A novel role for embigin to promote sprouting of motor nerve terminals at the neuromuscular junction.
Lain E, Carnejac S, Escher P, Wilson MC, L?mo T, Gajendran N, Brenner HR
The Journal of biological chemistry284(13)8930-9(2009 Mar)

Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70).
Wilson MC, Meredith D, Fox JE, Manoharan C, Davies AJ, Halestrap AP
The Journal of biological chemistry280(29)27213-21(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression of active MCT2 at the plasma membrane of oocytes was significantly enhanced by co-expression of exogenous embigin.
Title: The inhibition of monocarboxylate transporter 2 (MCT2) by AR-C155858 is modulated by the associated ancillary protein.

embigin enhances neural cell adhesion molecule-dependent neuromuscular adhesion and thereby modulates neuromuscular junction formation and plasticity.
Title: A novel role for embigin to promote sprouting of motor nerve terminals at the neuromuscular junction.

MCT2 associates with gp70 and is insensitive to pCMBS; embigin (gp70) is an ancillary protein required to maintain the catalytic activity of MCT2
Title: Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70).

The following Emb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Emb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10406	NM_053719.1 Latest version!	Rattus norvegicus embigin (Emb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Emb ( NM_053719.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446171.1 Rattus norvegicus embigin (Emb), mRNA.

Price & Availability↑

CloneID	ORa10406
Clone ID Related Accession (Same CDS sequence)	NM_053719.1
Accession Version	NM_053719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-06
Organism	Rattus norvegicus(Norway rat)
Product	embigin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ009698.1. ##Evidence-Data-START## Transcript exon combination :: AJ009698.1, BC061846.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCGCTCGC ACACTGGCTT GAGGGCGCTT GTGGCGCCTG GTTGCTCGCT CCTCCTTCTA 
TATCTTCTGG CGGCGACGCG CCCGGATCGG GCTGTGGGCG ATCCCGCAGA CTCAGCGTTT 
ACAAGTCTAC CTGTTCGAGA GGAAATGATG GCTAAGTACG CAAATCTGTC CTTGGAGACC 
TATAATATCT CACTGACAGA ACAGACCAGA GTATCAGAAC AAAACATAAC TTTGGAAAGA 
CCTTCTCACC TGGAACTCGA ATGCACGTTC ACGGCAACTG AGGATGTGAT GTCAATGAAT 
GTGACTTGGA AGAAAGATGA CGCGCTCCTT GAGACTACTG ATGGTTTCAA TACAACTAAA 
ATGGGGGACA CCTTATACAG TCAATACAGG TTCACCGTTT TTAATAGCAA ACAAATGGGA 
AAATACTCTT GTTTCCTTGG AGAAGAGCTC AGAGGAACCT TTAATATCAG AGTACCCAAA 
GTTCATGGGA AAAACAAGCC TTTGATCACT TACGTGGGGG ATTCTACTGT GCTGAAGTGT 
GAATGTCAAA ATTGTCTTCC TTTAAATTGG ACTTGGTACA TGAGTAATGG AACTGCACAG 
GTTCCCATCG ATGTTCACGT GAATGATAAG TTTGATATAA ATGGTTCCTA TGCTAACGAA 
ACAAAGCTCA AGGTAAAGCA CCTTTTGGAG GAAGATGGAG GATCCTACTG GTGCCGGGCA 
GCCTTCCCGT TAGGGGAGAG TGAGGAACAC ATTAAGCTGG TTGTGCTGAG TTTCATGGTG 
CCTCTCAAGC CATTCCTTGC CATAATTGCT GAAGTCATCC TCTTAGTGGC CATCATCCTG 
CTTTGTGAAG TGTACACGCA GAAGAAAAAG AATGACCCAG ATGATGGGAA AGAATTTGAA 
CAAATCGAAC AGTTGAAATC AGATGATAGC AATGGAATAG AAAACAATGT CCCCCGGTAC 
AGAAAAACGG ACTCTGGGGA TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446171.1
CDS	91..1077
Translation	MRSHTGLRALVAPGCSLLLLYLLAATRPDRAVGDPADSAFTSLPVREEMMAKYANLSLETYNISLTEQTRVSEQNITLERPSHLELECTFTATEDVMSMNVTWKKDDALLETTDGFNTTKMGDTLYSQYRFTVFNSKQMGKYSCFLGEELRGTFNIRVPKVHGKNKPLITYVGDSTVLKCECQNCLPLNWTWYMSNGTAQVPIDVHVNDKFDINGSYANETKLKVKHLLEEDGGSYWCRAAFPLGESEEHIKLVVLSFMVPLKPFLAIIAEVILLVAIILLCEVYTQKKKNDPDDGKEFEQIEQLKSDDSNGIENNVPRYRKTDSGDQ

Target ORF information:

RefSeq Version	NM_053719.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus embigin (Emb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053719.1

ORF Insert Sequence:

ATGCGCTCGC ACACTGGCTT GAGGGCGCTT GTGGCGCCTG GTTGCTCGCT CCTCCTTCTA 
TATCTTCTGG CGGCGACGCG CCCGGATCGG GCTGTGGGCG ATCCCGCAGA CTCAGCGTTT 
ACAAGTCTAC CTGTTCGAGA GGAAATGATG GCTAAGTACG CAAATCTGTC CTTGGAGACC 
TATAATATCT CACTGACAGA ACAGACCAGA GTATCAGAAC AAAACATAAC TTTGGAAAGA 
CCTTCTCACC TGGAACTCGA ATGCACGTTC ACGGCAACTG AGGATGTGAT GTCAATGAAT 
GTGACTTGGA AGAAAGATGA CGCGCTCCTT GAGACTACTG ATGGTTTCAA TACAACTAAA 
ATGGGGGACA CCTTATACAG TCAATACAGG TTCACCGTTT TTAATAGCAA ACAAATGGGA 
AAATACTCTT GTTTCCTTGG AGAAGAGCTC AGAGGAACCT TTAATATCAG AGTACCCAAA 
GTTCATGGGA AAAACAAGCC TTTGATCACT TACGTGGGGG ATTCTACTGT GCTGAAGTGT 
GAATGTCAAA ATTGTCTTCC TTTAAATTGG ACTTGGTACA TGAGTAATGG AACTGCACAG 
GTTCCCATCG ATGTTCACGT GAATGATAAG TTTGATATAA ATGGTTCCTA TGCTAACGAA 
ACAAAGCTCA AGGTAAAGCA CCTTTTGGAG GAAGATGGAG GATCCTACTG GTGCCGGGCA 
GCCTTCCCGT TAGGGGAGAG TGAGGAACAC ATTAAGCTGG TTGTGCTGAG TTTCATGGTG 
CCTCTCAAGC CATTCCTTGC CATAATTGCT GAAGTCATCC TCTTAGTGGC CATCATCCTG 
CTTTGTGAAG TGTACACGCA GAAGAAAAAG AATGACCCAG ATGATGGGAA AGAATTTGAA 
CAAATCGAAC AGTTGAAATC AGATGATAGC AATGGAATAG AAAACAATGT CCCCCGGTAC 
AGAAAAACGG ACTCTGGGGA TCAGTGA