Map2k6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map2k6
Entrez Gene ID	114495
Full Name	mitogen-activated protein kinase kinase 6
Synonyms	Mkk6
General protein information	Preferred Names mitogen-activated protein kinase kinase 6 Names dual specificity mitogen-activated protein kinase kinase 6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	activator of p38 MAPK; may play a role in cardiac hypertrophic and failure conditions [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053703.3	NP_446155.1	dual specificity mitogen-activated protein kinase kinase 6

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAP2K6	NP_002749.2
	MAP2K6	XP_001083492.1
Caenorhabditis elegans (roundworm)	sek-1	NP_509322.2
Canis lupus familiaris (dog)	MAP2K6	XP_005624348.1
Rattus norvegicus (Norway rat)	Map2k6	NP_446155.1
Xenopus tropicalis (tropical clawed frog)	map2k6	NP_001005653.1
Bos taurus (cattle)	MAP2K6	NP_001029217.1
Gallus gallus (chicken)	MAP2K6	XP_003642396.1
Danio rerio (zebrafish)	map2k6	NP_571799.1
Pan troglodytes (chimpanzee)	MAP2K6	XP_523699.2
Mus musculus (house mouse)	Map2k6	NP_036073.1

Gene Ontology
Bibliography

Related articles in PubMed

MKK6 controls T3-mediated browning of white adipose tissue.
Matesanz N, Bernardo E, Ac?n-P?rez R, Manieri E, P?rez-Sieira S, Hern?ndez-Cosido L, Montalvo-Romeral V, Mora A, Rodr?guez E, Leiva-Vega L, Lechuga-Vieco AV, Ruiz-Cabello J, Torres JL, Crespo-Ruiz M, Centeno F, ?lvarez CV, Marcos M, Enr?quez JA, Nogueiras R, Sabio G
Nature communications8(1)856(2017 10)

?-Amyloid-evoked apoptotic cell death is mediated through MKK6-p66shc pathway.
Bashir M, Parray AA, Baba RA, Bhat HF, Bhat SS, Mushtaq U, Andrabi KI, Khanday FA
Neuromolecular medicine16(1)137-49(2014 Mar)

Precisely ordered phosphorylation reactions in the p38 mitogen-activated protein (MAP) kinase cascade.
Humphreys JM, Piala AT, Akella R, He H, Goldsmith EJ
The Journal of biological chemistry288(32)23322-30(2013 Aug)

Pro-life role for c-Jun N-terminal kinase and p38 mitogen-activated protein kinase at rostral ventrolateral medulla in experimental brain stem death.
Chang AY
Journal of biomedical science1996(2012 Nov)

p38 kinase is crucial for osteopontin-induced furin expression that supports cervical cancer progression.
Kumar V, Behera R, Lohite K, Karnik S, Kundu GC
Cancer research70(24)10381-91(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Serine phosphorylation of p66shc is carried out by active MKK6. beta-Amyloid-induced ROS production and apoptosis increased in the presence of MKK6 and p66shc, which directly associate.
Title: ?-Amyloid-evoked apoptotic cell death is mediated through MKK6-p66shc pathway.

The models confirmed the reaction order, revealed processivity in the phosphorylation of MEK6 by ASK1, and suggested that the order of phosphorylation is dictated by both binding and catalysis rates.
Title: Precisely ordered phosphorylation reactions in the p38 mitogen-activated protein (MAP) kinase cascade.

Rap1, MKK3/6, and p38 MAPK have a role in the induction of mGluR-dependent LTD by directly coupling to receptor trafficking machineries to facilitate the loss of synaptic AMPA receptors
Title: Rap1-induced p38 mitogen-activated protein kinase activation facilitates AMPA receptor trafficking via the GDI.Rab5 complex. Potential role in (S)-3,5-dihydroxyphenylglycene-induced long term depression.

The following Map2k6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map2k6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10358	NM_053703.3 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase 6 (Map2k6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map2k6 ( NM_053703.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446155.1 Rattus norvegicus mitogen-activated protein kinase kinase 6 (Map2k6), mRNA.

Price & Availability↑

CloneID	ORa10358
Clone ID Related Accession (Same CDS sequence)	NM_053703.3
Accession Version	NM_053703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	dual specificity mitogen-activated protein kinase kinase 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF369384.1. On Jun 10, 2007 this sequence version replaced NM_053703.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369384.1, BC087004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCAGT CGAAAGGCAA GAAGCGAAAC CCCGGCCTTA AGATTCCAAG GGAAGCGTTT 
GAACAACCTC AGACCAGTTC CACGCCGCCT CGGGATTTAG ACTCCAAGGC TTGCATTTCT 
ATTGGAAACC AGAACTTTGA GGTGAAGGCC GATGACTTGG AGCCTATAGT GGAGCTGGGA 
CGAGGTGCGT ACGGGGTGGT GGAGAAGATG CGTCACGTGC CCAGCGGGCA GATCATGGCG 
GTGAAGCGGA TACGGGCCAC AGTTAATAGC CAGGAGCAGA AACGGCTACT GATGGATCTG 
GATGTTTCTA TGAGGACAGT GGACTGTCCG TTTACCGTGA CCTTCTATGG TGCACTCTTC 
CGGGAGGGCG ATGTGTGGAT CTGCATGGAG CTCATGGATA CGTCACTAGA TAAATTCTAC 
AAACAAGTTA TTGATAAAGG CCAAACAATT CCAGAAGATA TCTTAGGGAA GATAGCAGTT 
TCTATTGTAA AAGCATTAGA ACATTTACAC AGTAAGCTCT CTGTTATCCA TCGAGATGTC 
AAGCCTTCAA ATGTGCTCAT TAACACGCTG GGCCAAGTGA AGATGTGTGA CTTTGGAATC 
AGCGGCTACC TGGTAGACTC TGTTGCGAAA ACGATCGATG CCGGTTGCAA ACCATACATG 
GCTCCTGAAC GAATAAATCC AGAGCTCAAC CAGAAGGGGT ACAGTGTGAA GTCTGACATT 
TGGAGTCTGG GCATCACCAT GATTGAGCTG GCCATCCTCC GGTTTCCCTA TGATTCTTGG 
GGAACGCCCT TCCAGCAGCT AAAACAGGTG GTAGAAGAGC CGTCTCCACA ACTCCCAGCG 
GACAAGTTCT CTGCTGACTT CGTTGACTTT ACCTCACAGT GCTTGAAGAA AAATTCCAAA 
GAACGGCCCA CGTATCCAGA GCTTATGCAA CATCCATTTT TCACCGTCCA TGAAGCCAAA 
GCAGCAGATG TGGCGTCTTT CGTAAAACTG ATACTCGGGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446155.1
CDS	108..1112
Translation	MSQSKGKKRNPGLKIPREAFEQPQTSSTPPRDLDSKACISIGNQNFEVKADDLEPIVELGRGAYGVVEKMRHVPSGQIMAVKRIRATVNSQEQKRLLMDLDVSMRTVDCPFTVTFYGALFREGDVWICMELMDTSLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINTLGQVKMCDFGISGYLVDSVAKTIDAGCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSADFVDFTSQCLKKNSKERPTYPELMQHPFFTVHEAKAADVASFVKLILGD

Target ORF information:

RefSeq Version	NM_053703.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase 6 (Map2k6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053703.3

ORF Insert Sequence:

ATGTCTCAGT CGAAAGGCAA GAAGCGAAAC CCCGGCCTTA AGATTCCAAG GGAAGCGTTT 
GAACAACCTC AGACCAGTTC CACGCCGCCT CGGGATTTAG ACTCCAAGGC TTGCATTTCT 
ATTGGAAACC AGAACTTTGA GGTGAAGGCC GATGACTTGG AGCCTATAGT GGAGCTGGGA 
CGAGGTGCGT ACGGGGTGGT GGAGAAGATG CGTCACGTGC CCAGCGGGCA GATCATGGCG 
GTGAAGCGGA TACGGGCCAC AGTTAATAGC CAGGAGCAGA AACGGCTACT GATGGATCTG 
GATGTTTCTA TGAGGACAGT GGACTGTCCG TTTACCGTGA CCTTCTATGG TGCACTCTTC 
CGGGAGGGCG ATGTGTGGAT CTGCATGGAG CTCATGGATA CGTCACTAGA TAAATTCTAC 
AAACAAGTTA TTGATAAAGG CCAAACAATT CCAGAAGATA TCTTAGGGAA GATAGCAGTT 
TCTATTGTAA AAGCATTAGA ACATTTACAC AGTAAGCTCT CTGTTATCCA TCGAGATGTC 
AAGCCTTCAA ATGTGCTCAT TAACACGCTG GGCCAAGTGA AGATGTGTGA CTTTGGAATC 
AGCGGCTACC TGGTAGACTC TGTTGCGAAA ACGATCGATG CCGGTTGCAA ACCATACATG 
GCTCCTGAAC GAATAAATCC AGAGCTCAAC CAGAAGGGGT ACAGTGTGAA GTCTGACATT 
TGGAGTCTGG GCATCACCAT GATTGAGCTG GCCATCCTCC GGTTTCCCTA TGATTCTTGG 
GGAACGCCCT TCCAGCAGCT AAAACAGGTG GTAGAAGAGC CGTCTCCACA ACTCCCAGCG 
GACAAGTTCT CTGCTGACTT CGTTGACTTT ACCTCACAGT GCTTGAAGAA AAATTCCAAA 
GAACGGCCCA CGTATCCAGA GCTTATGCAA CATCCATTTT TCACCGTCCA TGAAGCCAAA 
GCAGCAGATG TGGCGTCTTT CGTAAAACTG ATACTCGGGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MKK6 controls T3-mediated browning of white adipose tissue.
Nature communications8(1)856(2017 10)
Matesanz N,Bernardo E,Ac?n-P?rez R,Manieri E,P?rez-Sieira S,Hern?ndez-Cosido L,Montalvo-Romeral V,Mora A,Rodr?guez E,Leiva-Vega L,Lechuga-Vieco AV,Ruiz-Cabello J,Torres JL,Crespo-Ruiz M,Centeno F,?lvarez CV,Marcos M,Enr?quez JA,Nogueiras R,Sabio G

?-Amyloid-evoked apoptotic cell death is mediated through MKK6-p66shc pathway.
Neuromolecular medicine16(1)137-49(2014 Mar)
Bashir M,Parray AA,Baba RA,Bhat HF,Bhat SS,Mushtaq U,Andrabi KI,Khanday FA

Precisely ordered phosphorylation reactions in the p38 mitogen-activated protein (MAP) kinase cascade.
The Journal of biological chemistry288(32)23322-30(2013 Aug)
Humphreys JM,Piala AT,Akella R,He H,Goldsmith EJ

Pro-life role for c-Jun N-terminal kinase and p38 mitogen-activated protein kinase at rostral ventrolateral medulla in experimental brain stem death.
Journal of biomedical science1996(2012 Nov)
Chang AY

p38 kinase is crucial for osteopontin-induced furin expression that supports cervical cancer progression.
Cancer research70(24)10381-91(2010 Dec)
Kumar V,Behera R,Lohite K,Karnik S,Kundu GC