Hcn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hcn2
Entrez Gene ID	114244
Full Name	hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2
General protein information	Preferred Names hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 Names potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 hyperpolarization activated cyclic nucleotide-gated potassium channel 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	may play a role in generation of neuronal pacemaker activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053684.1	NP_446136.1	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCN2	NP_001185.3
Gallus gallus (chicken)	HCN2	XP_003642915.1
Xenopus tropicalis (tropical clawed frog)	hcn2	XP_002939815.2
Canis lupus familiaris (dog)	HCN2	XP_005642548.1
Rattus norvegicus (Norway rat)	Hcn2	NP_446136.1
Pan troglodytes (chimpanzee)	LOC467640	XP_003952669.1
Bos taurus (cattle)	HCN2	XP_002689207.2
Mus musculus (house mouse)	Hcn2	NP_032252.1
Danio rerio (zebrafish)	hcn2b	XP_685414.3

Gene Ontology
Bibliography

Related articles in PubMed

Hyperpolarization-activated cyclic nucleotide-gated channels in peripheral diaphragmatic lymphatics.
Negrini D, Marcozzi C, Solari E, Bossi E, Cinquetti R, Reguzzoni M, Moriondo A
American journal of physiology. Heart and circulatory physiology311(4)H892-H903(2016 Oct)

Expression and cellular localization of HCN channels in rat cerebellar granule neurons.
Z??iga R, Gonz?lez D, Valenzuela C, Brown N, Z??iga L
Biochemical and biophysical research communications478(3)1429-35(2016 09)

Inhibition of HCN channel activity in the thalamus attenuates chronic pain in rats.
Ding W, You Z, Shen S, Chen L, Zhu S, Mao J
Neuroscience letters63197-103(2016 Sep)

Age-associated expression of HCN channel isoforms in rat sinoatrial node.
Huang X, Yang P, Yang Z, Zhang H, Ma A
Experimental biology and medicine (Maywood, N.J.)241(3)331-9(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that HCN channels might play an important role in affecting contraction frequency of lymphatic muscle cells.
Title: Hyperpolarization-activated cyclic nucleotide-gated channels in peripheral diaphragmatic lymphatics.

the expression and relative abundances of HCN channels in cerebellum and primary cultures of cerebellar granule neurons, is described.
Title: Expression and cellular localization of HCN channels in rat cerebellar granule neurons.

Hyperpolarization-activated cyclic nucleotide-gated (HCN) 2 protein expression levels were increased in the thalamus under chronic pain condition. Results suggest, increased HCN channel activity in the thalamus of the ascending nociceptive pathway contributes to both chronic neuropathic and inflammatory pain conditions.
Title: Inhibition of HCN channel activity in the thalamus attenuates chronic pain in rats.

During the senescent process, the HCN2 and HCN4 protein levels declined.
Title: Age-associated expression of HCN channel isoforms in rat sinoatrial node.

Spironolactone increased miRNA-1 expression in myocardium after myocardial infarction. Upregulation of miRNA-1 expression contributed to the posttranscriptional repression of HCN2/4 expression.
Title: Spironolactone Regulates HCN Protein Expression Through Micro-RNA-1 in Rats With Myocardial Infarction.

The following Hcn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10305	NM_053684.1 Latest version!	Rattus norvegicus hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (Hcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hcn2 ( NM_053684.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446136.1 Rattus norvegicus hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (Hcn2), mRNA.

Price & Availability↑

CloneID	ORa10305
Clone ID Related Accession (Same CDS sequence)	NM_053684.1
Accession Version	NM_053684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Rattus norvegicus(Norway rat)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB164197.1. On Aug 30, 2004 this sequence version replaced XM_343170.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB164197.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMN00632254 [ECO:0000348] ##Evidence-Data-END##

ATGGATGCGC GCGGGGGCGG CGGGCGGCCG GGCGATAGTC CGGGCGCGAC CCCTGCGCCG 
GGGCCGCCGC CACCGCCGCC GCCGCCCGCG CCCCCTCAGC CTCAGCCACC ACCCGCGCCA 
CCCCCGAACC CCACGACCCC CTCGCACCCG GAGTCGGCGG ACGAGCCCGG CCCGCGCTCC 
CGGCTCTGCA GCCGCGACAG CTCCTGCACT CCTGGCGCGG CCAAGGGCGG CGCGAATGGC 
GAGTGCGGGC GCGGGGAGCC GCAGTGCAGC CCCGAGGGCC CCGCGCGCGG CCCCAAGGTT 
TCGTTCTCAT GTCGCGGGGC GGCCTCGGGG CCCGCGGCGG CCGAGGAGGC GGGCAGCGAG 
GAGGCGGGCC CGGCGGGTGA GCCGCGCGGC AGCCAGGCCA GCTTCCTGCA GCGCCAATTC 
GGGGCGCTCC TGCAGCCGGG CGTCAACAAG TTCTCCCTGC GGATGTTTGG CAGCCAGAAG 
GCCGTGGAGC GCGAGCAGGA ACGCGTGAAG TCGGCGGGGG CCTGGATCAT CCACCCCTAC 
AGCGACTTCA GGTTCTACTG GGACTTCACC ATGCTGTTGT TCATGGTGGG AAATCTCATC 
ATCATCCCTG TGGGCATCAC TTTCTTCAAG GACGAGACCA CGGCGCCCTG GATCGTCTTC 
AACGTGGTCT CGGACACTTT CTTCCTCATG GACTTGGTGC TGAACTTTCG CACCGGCATT 
GTTATTGAGG ACAACACGGA GATCATCCTG GACCCCGAAA AGATAAAGAA AAAGTACCTG 
CGTACGTGGT TCGTGGTAGA CTTCGTGTCA TCCATCCCGG TGGACTACAT CTTCCTCATC 
GTGGAGAAGG GAATCGACTC CGAGGTCTAC AAGACGGCCC GTGCACTACG CATCGTGCGT 
TTCACCAAGA TCCTCAGTCT GCTGCGGTTG CTGCGGCTAT CCCGGCTCAT CCGATATATC 
CACCAATGGG AGGAGATTTT CCACATGACC TACGACCTGG CAAGCGCGGT GATGCGCATC 
TGTAACCTGA TCAGCATGAT GCTGCTGCTC TGCCACTGGG ACGGCTGCCT GCAGTTCCTG 
GTGCCCATGC TGCAAGACTT CCCCAGCGAC TGCTGGGTGT CCATCAACAA CATGGTGAAC 
CACTCGTGGA GCGAACTCTA TTCGTTCGCG CTCTTCAAGG CCATGAGCCA CATGCTCTGT 
ATTGGCTACG GGCGGCAGGC TCCCGAGAGC ATGACGGACA TCTGGCTCAC CATGCTCAGC 
ATGATCGTGG GCGCCACCTG CTACGCTATG TTCATTGGGC ACGCCACGGC GCTTATCCAG 
TCCCTGGACT CGTCACGGCG CCAGTACCAG GAGAAGTACA AGCAAGTGGA GCAGTACATG 
TCCTTCCACA AACTGCCGGC TGACTTCCGC CAGAAGATCC ACGATTACTA TGAACACCGG 
TACCAGGGGA AGATGTTTGA CGAGGACAGC ATCCTGGGGG AACTCAACGG CCCACTGCGT 
GAGGAGATTG TGAACTTCAA CTGCCGGAAG CTGGTGGCTT CCATGCCGTT GTTTGCCAAC 
GCAGACCCCA ACTTCGTCAC CGCCATGCTG ACAAAGCTCA AATTTGAGGT CTTCCAGCCT 
GGAGACTACA TCATCCGAGA GGGGACCATC GGGAAGAAGA TGTACTTCAT CCAGCACGGG 
GTGGTGAGCG TGCTCACCAA GGGCAACAAG GAGATGAAGC TGTCAGATGG CTCCTATTTT 
GGGGAGATCT GCCTGCTCAC GAGGGGCCGG CGCACAGCCA GTGTGCGGGC TGACACCTAC 
TGTCGCCTCT ACTCACTGAG CGTGGACAAC TTCAACGAGG TGCTGGAGGA GTACCCCATG 
ATGCGGCGTG CCTTTGAGAC CGTGGCCATT GACCGCCTGG ACCGCATAGG CAAGAAGAAC 
TCCATCTTGC TACACAAGGT TCAGCATGAT CTCAGCTCGG GTGTGTTCAA CAACCAGGAG 
AACGCCATCA TCCAGGAGAT TGTCAAATAT GACCGTGAGA TGGTGCAGCA GGCAGAGCTG 
GGCCAGCGTG TGGGGCTCTT CCCACCACCG CCACCACCGC AGGTCACGTC GGCCATCGCC 
ACACTGCAGC AGGCCGTGGC CATGAGCTTC TGCCCGCAGG TGGCCCGCCC GCTCGTGGGG 
CCCCTGGCGC TAGGGTCCCC ACGCCTCGTG CGCCGCGCGC CCCCAGGGCC TCTGCCTCCT 
GCAGCCTCAC CAGGGCCACC CGCAGCGAGC CCCCCGGCTG CACCCTCGAG CCCTCGGGCA 
CCGCGGACCT CACCCTACGG TGTGCCTGGC TCTCCGGCAA CGCGTGTGGG GCCCGCATTG 
CCCGCACGCC GCCTGAGCCG CGCCTCGCGC CCACTGTCCG CCTCGCAGCC CTCGCTGCCC 
CACGGCGCGC CCGCACCCAG CCCCGCGGCC TCTGCGCGCC CGGCCAGCAG CTCCACCCCG 
CGTCTGGGAC CCGCACCCAC CACCCGGACC GCGGCACCCA GTCCGGACCG CAGGGACTCA 
GCCTCGCCGG GCGCTGCCAG TGGCCTCGAC CCACTGGACT CTGCGCGCTC GCGCCTCTCT 
TCCAACTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446136.1
CDS	36..2627
Translation	MDARGGGGRPGDSPGATPAPGPPPPPPPPAPPQPQPPPAPPPNPTTPSHPESADEPGPRSRLCSRDSSCTPGAAKGGANGECGRGEPQCSPEGPARGPKVSFSCRGAASGPAAAEEAGSEEAGPAGEPRGSQASFLQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPSDCWVSINNMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLSSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPQVTSAIATLQQAVAMSFCPQVARPLVGPLALGSPRLVRRAPPGPLPPAASPGPPAASPPAAPSSPRAPRTSPYGVPGSPATRVGPALPARRLSRASRPLSASQPSLPHGAPAPSPAASARPASSSTPRLGPAPTTRTAAPSPDRRDSASPGAASGLDPLDSARSRLSSNL

Target ORF information:

RefSeq Version	NM_053684.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (Hcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053684.1

ORF Insert Sequence:

ATGGATGCGC GCGGGGGCGG CGGGCGGCCG GGCGATAGTC CGGGCGCGAC CCCTGCGCCG 
GGGCCGCCGC CACCGCCGCC GCCGCCCGCG CCCCCTCAGC CTCAGCCACC ACCCGCGCCA 
CCCCCGAACC CCACGACCCC CTCGCACCCG GAGTCGGCGG ACGAGCCCGG CCCGCGCTCC 
CGGCTCTGCA GCCGCGACAG CTCCTGCACT CCTGGCGCGG CCAAGGGCGG CGCGAATGGC 
GAGTGCGGGC GCGGGGAGCC GCAGTGCAGC CCCGAGGGCC CCGCGCGCGG CCCCAAGGTT 
TCGTTCTCAT GTCGCGGGGC GGCCTCGGGG CCCGCGGCGG CCGAGGAGGC GGGCAGCGAG 
GAGGCGGGCC CGGCGGGTGA GCCGCGCGGC AGCCAGGCCA GCTTCCTGCA GCGCCAATTC 
GGGGCGCTCC TGCAGCCGGG CGTCAACAAG TTCTCCCTGC GGATGTTTGG CAGCCAGAAG 
GCCGTGGAGC GCGAGCAGGA ACGCGTGAAG TCGGCGGGGG CCTGGATCAT CCACCCCTAC 
AGCGACTTCA GGTTCTACTG GGACTTCACC ATGCTGTTGT TCATGGTGGG AAATCTCATC 
ATCATCCCTG TGGGCATCAC TTTCTTCAAG GACGAGACCA CGGCGCCCTG GATCGTCTTC 
AACGTGGTCT CGGACACTTT CTTCCTCATG GACTTGGTGC TGAACTTTCG CACCGGCATT 
GTTATTGAGG ACAACACGGA GATCATCCTG GACCCCGAAA AGATAAAGAA AAAGTACCTG 
CGTACGTGGT TCGTGGTAGA CTTCGTGTCA TCCATCCCGG TGGACTACAT CTTCCTCATC 
GTGGAGAAGG GAATCGACTC CGAGGTCTAC AAGACGGCCC GTGCACTACG CATCGTGCGT 
TTCACCAAGA TCCTCAGTCT GCTGCGGTTG CTGCGGCTAT CCCGGCTCAT CCGATATATC 
CACCAATGGG AGGAGATTTT CCACATGACC TACGACCTGG CAAGCGCGGT GATGCGCATC 
TGTAACCTGA TCAGCATGAT GCTGCTGCTC TGCCACTGGG ACGGCTGCCT GCAGTTCCTG 
GTGCCCATGC TGCAAGACTT CCCCAGCGAC TGCTGGGTGT CCATCAACAA CATGGTGAAC 
CACTCGTGGA GCGAACTCTA TTCGTTCGCG CTCTTCAAGG CCATGAGCCA CATGCTCTGT 
ATTGGCTACG GGCGGCAGGC TCCCGAGAGC ATGACGGACA TCTGGCTCAC CATGCTCAGC 
ATGATCGTGG GCGCCACCTG CTACGCTATG TTCATTGGGC ACGCCACGGC GCTTATCCAG 
TCCCTGGACT CGTCACGGCG CCAGTACCAG GAGAAGTACA AGCAAGTGGA GCAGTACATG 
TCCTTCCACA AACTGCCGGC TGACTTCCGC CAGAAGATCC ACGATTACTA TGAACACCGG 
TACCAGGGGA AGATGTTTGA CGAGGACAGC ATCCTGGGGG AACTCAACGG CCCACTGCGT 
GAGGAGATTG TGAACTTCAA CTGCCGGAAG CTGGTGGCTT CCATGCCGTT GTTTGCCAAC 
GCAGACCCCA ACTTCGTCAC CGCCATGCTG ACAAAGCTCA AATTTGAGGT CTTCCAGCCT 
GGAGACTACA TCATCCGAGA GGGGACCATC GGGAAGAAGA TGTACTTCAT CCAGCACGGG 
GTGGTGAGCG TGCTCACCAA GGGCAACAAG GAGATGAAGC TGTCAGATGG CTCCTATTTT 
GGGGAGATCT GCCTGCTCAC GAGGGGCCGG CGCACAGCCA GTGTGCGGGC TGACACCTAC 
TGTCGCCTCT ACTCACTGAG CGTGGACAAC TTCAACGAGG TGCTGGAGGA GTACCCCATG 
ATGCGGCGTG CCTTTGAGAC CGTGGCCATT GACCGCCTGG ACCGCATAGG CAAGAAGAAC 
TCCATCTTGC TACACAAGGT TCAGCATGAT CTCAGCTCGG GTGTGTTCAA CAACCAGGAG 
AACGCCATCA TCCAGGAGAT TGTCAAATAT GACCGTGAGA TGGTGCAGCA GGCAGAGCTG 
GGCCAGCGTG TGGGGCTCTT CCCACCACCG CCACCACCGC AGGTCACGTC GGCCATCGCC 
ACACTGCAGC AGGCCGTGGC CATGAGCTTC TGCCCGCAGG TGGCCCGCCC GCTCGTGGGG 
CCCCTGGCGC TAGGGTCCCC ACGCCTCGTG CGCCGCGCGC CCCCAGGGCC TCTGCCTCCT 
GCAGCCTCAC CAGGGCCACC CGCAGCGAGC CCCCCGGCTG CACCCTCGAG CCCTCGGGCA 
CCGCGGACCT CACCCTACGG TGTGCCTGGC TCTCCGGCAA CGCGTGTGGG GCCCGCATTG 
CCCGCACGCC GCCTGAGCCG CGCCTCGCGC CCACTGTCCG CCTCGCAGCC CTCGCTGCCC 
CACGGCGCGC CCGCACCCAG CCCCGCGGCC TCTGCGCGCC CGGCCAGCAG CTCCACCCCG 
CGTCTGGGAC CCGCACCCAC CACCCGGACC GCGGCACCCA GTCCGGACCG CAGGGACTCA 
GCCTCGCCGG GCGCTGCCAG TGGCCTCGAC CCACTGGACT CTGCGCGCTC GCGCCTCTCT 
TCCAACTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hyperpolarization-activated cyclic nucleotide-gated channels in peripheral diaphragmatic lymphatics.
American journal of physiology. Heart and circulatory physiology311(4)H892-H903(2016 Oct)
Negrini D,Marcozzi C,Solari E,Bossi E,Cinquetti R,Reguzzoni M,Moriondo A

Expression and cellular localization of HCN channels in rat cerebellar granule neurons.
Biochemical and biophysical research communications478(3)1429-35(2016 09)
Z??iga R,Gonz?lez D,Valenzuela C,Brown N,Z??iga L

Inhibition of HCN channel activity in the thalamus attenuates chronic pain in rats.
Neuroscience letters63197-103(2016 Sep)
Ding W,You Z,Shen S,Chen L,Zhu S,Mao J

Age-associated expression of HCN channel isoforms in rat sinoatrial node.
Experimental biology and medicine (Maywood, N.J.)241(3)331-9(2016 Feb)
Huang X,Yang P,Yang Z,Zhang H,Ma A