Nemf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nemf
Entrez Gene ID	100322884
Full Name	nuclear export mediator factor
Synonyms	Sdccag1
General protein information	Preferred Names nuclear export mediator factor Names nuclear export mediator factor NEMF serologically defined colon cancer antigen 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170586.1	NP_001164057.1	nuclear export mediator factor NEMF

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	Y82E9BR.18	NP_497411.2
Bos taurus (cattle)	NEMF	NP_001192434.1
Rattus norvegicus (Norway rat)	Nemf	NP_001164057.1
Drosophila melanogaster (fruit fly)	Clbn	NP_001163721.1
Arabidopsis thaliana (thale cress)	emb1441	NP_199804.1
Xenopus tropicalis (tropical clawed frog)	nemf	XP_002938173.2
Homo sapiens (human)	NEMF	NP_004704.2
Canis lupus familiaris (dog)	NEMF	XP_005623434.1
Mus musculus (house mouse)	Nemf	NP_079717.2
Pan troglodytes (chimpanzee)	NEMF	XP_509934.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002680	XP_312244.5
Schizosaccharomyces pombe (fission yeast)	SPCC132.01c	NP_588145.2
Oryza sativa (rice)	Os12g0564600	NP_001067042.1
Gallus gallus (chicken)	NEMF	NP_001239258.1

Gene Ontology
Bibliography

Related articles in PubMed

A brain-derived MeCP2 complex supports a role for MeCP2 in RNA processing.
Long SW, Ooi JY, Yau PM, Jones PL
Bioscience reports31(5)333-43(2011 Oct)

Drosophila caliban, a nuclear export mediator, can function as a tumor suppressor in human lung cancer cells.
Bi X, Jones T, Abbasi F, Lee H, Stultz B, Hursh DA, Mortin MA
Oncogene24(56)8229-39(2005 Dec)

Gene and alternative splicing annotation with AIR.
Florea L, Di Francesco V, Miller J, Turner R, Yao A, Harris M, Walenz B, Mobarry C, Merkulov GV, Charlab R, Dew I, Deng Z, Istrail S, Li P, Sutton G
Genome research15(1)54-66(2005 Jan)

Expression pattern of mammalian cochlea outer hair cell (OHC) mRNA: screening of a rat OHC cDNA library.
Harter C, Ripoll C, Lenoir M, Hamel CP, Rebillard G
DNA and cell biology18(1)1-10(1999 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nemf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nemf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa30210	NM_001170586.1 Latest version!	Rattus norvegicus nuclear export mediator factor (Nemf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nemf ( NM_001170586.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001164057.1 Rattus norvegicus nuclear export mediator factor (Nemf), mRNA.

Price & Availability↑

CloneID	ORa30210
Clone ID Related Accession (Same CDS sequence)	NM_001170586.1
Accession Version	NM_001170586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	nuclear export mediator factor NEMF
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB756993.1, CA512507.1, FQ205447.1, AAHX01044895.1, AAHX01044894.1, AAHX01044893.1 and CA507359.1. On or before May 13, 2010 this sequence version replaced XM_002726704.1, XM_002729650.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data from several strains because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGACCC GCTTCAGCAC TGTTGACCTC CGCGCGGTGC TCGCGGAACT GAATGCGAAC 
TTGCTAGGAA TGAGAGTAAA CAACGTTTAT GATGTGGATA ACAAGACGTA TCTTATTCGG 
CTTCAAAATC CTGATTTTAA AGCTACACTG TTACTTGAAT CTGGTATACG AATTCACACA 
ACAGAATTTG AGTGGCCTAA GAACATGATG CCGTCTAGTT TTGCTATGAA GTGTCGAAAG 
CATTTGAAAA GTCGGAGATT AGTCAGTGCA AAACAGCTTG GTGTGGATAG AATTGTGGAT 
TTCCAATTTG GAAGTGATGA AGCTGCTTAT CACTTAATCA TTGAGCTCTA TGACAGGGGC 
AACATTGTCC TTACAGATTA TGAGTACCTA ATTTTAAATA TCCTAAGGTT TCGAACTGAT 
GAAGCTGATG ATGTTAAGTT TGCTGTTCGT GAACGTTATC CCATAGATCA TGCCAGAGCT 
GCTGAACCTT TGCTCACTTT GGAAAGATTG ACTGAAGTAA TAGCTAGAGC TCCTAGAGGT 
GAACTGCTGA AGAGAGTTCT GAATCCGTTA CTTCCCTATG GGCCAGCTCT TATTGAGCAT 
TGTCTTATAG AAAATGGATT CTCTGGCAAT GTCAAAGTGG ACGAAAAACT TGAAAGCAAA 
GATATTGAGA AGATACTGGT TTGTGTGCAG AGGGCAGAAG ACTATTTGGA AAAAACAGCC 
AACTTCAATG GAAAGGGATA TATAATTCAG AAAAGAGAGG TAAAACCAAG CCTGGATGCC 
AATAAACCAG CTGAAGACAT ACTCACGTAT GAGGAATTTC ATCCTTTCTT GTTTTCTCAA 
CATTTACAAT GTCCATATAT AGAATTCGAA TCATTTGACA AGGCTGTGGA TGAATTTTAT 
TCCAAGATAG AAGGTCAGAA AATTGACTTA AAAGCATTGC AACAGGAAAA ACAAGCACTG 
AAGAAATTAG ATAATGTCCG AAAGGATCAT GAAAACAGAT TGGAAGCTCT TCAGCAGGCT 
CAGGAAATTG ACAAACTGAA AGGGGAGCTC ATAGAGATGA ACCTGCAGAT AGTTGACAGA 
GCCATTCAGG TAGTTCGAAG TGCATTAGCC AACCAGATAG ATTGGACAGA AATTGGAGTG 
ATTGTGAAAG AAGCACAAGC CCAGGGAGAC CCTGTTGCAA GTGCAATCAA AGAACTAAAA 
CTGCAAACAA ACCACATTAC CATGCTTCTA CGAAATCCAT ACTTGTTATC AGAGGAGGAA 
GATGGTGATG GTGATGGCAG CATTGAGAAC AGTGATGCTG AAGCACCAAA AGGGAAAAAA 
AAAAAGCAAA AGAACAAGCA GCTGCAGAAG CCTCAGAAGA ACAAGCCGCT GCTTGTCGAT 
GTGGACCTCA GCCTGTCAGC CTATGCCAAT GCCAAAAAGT ATTATGATCA TAAGAGGTAT 
GCTGCTAAAA AAACACAGAG AACTGTCGAA GCTGCTGAGA AGGCATTCAA ATCAGCAGAG 
AAGAAAACAA AGCAAACCTT AAAAGAAGTA CAAACAGTTA CTTCTATCCA AAAAGCAAGG 
AAAGTGTATT GGTTTGAGAA ATTTCTGTGG TTTATTAGTT CAGAGAACTA TCTCATTATA 
GGTGGTCGAG ATCAGCAACA GAATGAGATT ATTGTGAAAA GATACTTAAC ACCAGGAGAC 
ATTTATGTGC ATGCTGATCT TCATGGAGCT ACCAGCTGTG TAATTAAGAA TCCAACAGGA 
GATCCCATCC CTCCTCGGAC TTTGACTGAA GCAGGCACAA TGGCACTTTG CTACAGCGCA 
GCTTGGGATG CCCGTGTTAT CACGAGTGCT TGGTGGGTGC ACCATCATCA GGTCTCTAAA 
ACAGCACCGA CAGGAGAGTA CTTAACAACT GGAAGCTTCA TGATAAGAGG AAAAAAGAAT 
TTCCTTCCTC CTTCATACCT AATGATGGGG TTTAGCTTCC TTTTTAAGGT AGATGAGTCC 
TGTGTCTGGA GACATCGAGG TGAACGAAAA GTCAGAGTGC AGGATGAAGA CATGGAAACA 
TTGACAAGTT GCACAAGTGA ACTCATGTCA GAAGAAATGG AACAGCTAGA AGGGGGCGAC 
AGCAGTGAAG AAGAGACCGA GGAGTTACGT GGAACCACGG AAGAAGTGGA ACTCAGGACT 
CAGGCTGATC CAGAGGACAT TGCTGCTCAC AGTGGAAGAG AGGAACTGAG CTCTGAAGAT 
GGAGAAGCCA TCAAAACAGC CACGAAAGAT GAAGAGCCCA TTGGTGAGGT GAGGGAAGAG 
GAGGAAGAGC CCTTCGAATA TCCTGACACC ACCATTGACT TGTCCCATCT TCAGTCCCAA 
AGGCCCCTAC AGAAACTGAC TCCAAGAGAA GAATCTTTTA ATTCAAGTGA CAGTAAATCA 
CAGAGCCGAA GACATTTGTC AGCCAAGGAG AGAAGAGAAA TGAAAAAGAA AAAGCTCCCA 
TGTGACTCAG GGGATTTAGA AGTGATAGAA GAAAAGGACA AAGAAAAAGA AAGTGCTGTG 
CACAGTGAAG CTGACCAGAA CACAAGCAAA AATGTGGCAG CTGGACAGCC AATGAAAAGA 
GGCCAGAAGA GTAAAATGAA AAAAATGAAG GAAAAATACA AAGACCAGGA TGATGAAGAT 
CGTGAACTTA TTATGAAATT GTTGGCATCT GCAGGTTCAA ACAAAGAAGA AAAGGGGAAG 
AAAGGAAAGA AAGGAAAAAC AAAAGATGAA CCCGTGAAAA AAAACCCACA GAAACCCAGA 
GGTGGACAGC GGGTTTTAGA TGTTGTTAAA GAAACCCCGT CCCTTCAGGC GTCGACTCCT 
GACTTACAAG ACTTTGCTGT GGATGAGCCA CATGATGACA AGGAAGAACA TGATCTGGAT 
CAGCAGGGAA ATGAGGAAAA TCTATTTGAC TCTTTGACAG GGCAACCACA TCCTGAAGAT 
GTATTAATGT TTGCTATTCC AATATGTGCT CCTTACACCA TCATGACAAA CTATAAATAC 
AAAGTGAAAC TTACTCCTGG AGTTCAGAAA AAGGGAAAAG CTGCAAAGAC AGCCTTGAAC 
AGTTTCATGC ACTCCAAAGA AGCAACAGCA AGAGAAAAAG ACTTATTCCG AAGTGTGAAG 
GACACAGATT TATCAAGAAA CATTCCCGGG AAAGTGAAAG TGTCTGCCCC CAATCTTCTG 
CACGTAAAAA GAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164057.1
CDS	51..3248
Translation	MKTRFSTVDLRAVLAELNANLLGMRVNNVYDVDNKTYLIRLQNPDFKATLLLESGIRIHTTEFEWPKNMMPSSFAMKCRKHLKSRRLVSAKQLGVDRIVDFQFGSDEAAYHLIIELYDRGNIVLTDYEYLILNILRFRTDEADDVKFAVRERYPIDHARAAEPLLTLERLTEVIARAPRGELLKRVLNPLLPYGPALIEHCLIENGFSGNVKVDEKLESKDIEKILVCVQRAEDYLEKTANFNGKGYIIQKREVKPSLDANKPAEDILTYEEFHPFLFSQHLQCPYIEFESFDKAVDEFYSKIEGQKIDLKALQQEKQALKKLDNVRKDHENRLEALQQAQEIDKLKGELIEMNLQIVDRAIQVVRSALANQIDWTEIGVIVKEAQAQGDPVASAIKELKLQTNHITMLLRNPYLLSEEEDGDGDGSIENSDAEAPKGKKKKQKNKQLQKPQKNKPLLVDVDLSLSAYANAKKYYDHKRYAAKKTQRTVEAAEKAFKSAEKKTKQTLKEVQTVTSIQKARKVYWFEKFLWFISSENYLIIGGRDQQQNEIIVKRYLTPGDIYVHADLHGATSCVIKNPTGDPIPPRTLTEAGTMALCYSAAWDARVITSAWWVHHHQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHRGERKVRVQDEDMETLTSCTSELMSEEMEQLEGGDSSEEETEELRGTTEEVELRTQADPEDIAAHSGREELSSEDGEAIKTATKDEEPIGEVREEEEEPFEYPDTTIDLSHLQSQRPLQKLTPREESFNSSDSKSQSRRHLSAKERREMKKKKLPCDSGDLEVIEEKDKEKESAVHSEADQNTSKNVAAGQPMKRGQKSKMKKMKEKYKDQDDEDRELIMKLLASAGSNKEEKGKKGKKGKTKDEPVKKNPQKPRGGQRVLDVVKETPSLQASTPDLQDFAVDEPHDDKEEHDLDQQGNEENLFDSLTGQPHPEDVLMFAIPICAPYTIMTNYKYKVKLTPGVQKKGKAAKTALNSFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLHVKRK

Target ORF information:

RefSeq Version	NM_001170586.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear export mediator factor (Nemf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170586.1

ORF Insert Sequence:

ATGAAGACCC GCTTCAGCAC TGTTGACCTC CGCGCGGTGC TCGCGGAACT GAATGCGAAC 
TTGCTAGGAA TGAGAGTAAA CAACGTTTAT GATGTGGATA ACAAGACGTA TCTTATTCGG 
CTTCAAAATC CTGATTTTAA AGCTACACTG TTACTTGAAT CTGGTATACG AATTCACACA 
ACAGAATTTG AGTGGCCTAA GAACATGATG CCGTCTAGTT TTGCTATGAA GTGTCGAAAG 
CATTTGAAAA GTCGGAGATT AGTCAGTGCA AAACAGCTTG GTGTGGATAG AATTGTGGAT 
TTCCAATTTG GAAGTGATGA AGCTGCTTAT CACTTAATCA TTGAGCTCTA TGACAGGGGC 
AACATTGTCC TTACAGATTA TGAGTACCTA ATTTTAAATA TCCTAAGGTT TCGAACTGAT 
GAAGCTGATG ATGTTAAGTT TGCTGTTCGT GAACGTTATC CCATAGATCA TGCCAGAGCT 
GCTGAACCTT TGCTCACTTT GGAAAGATTG ACTGAAGTAA TAGCTAGAGC TCCTAGAGGT 
GAACTGCTGA AGAGAGTTCT GAATCCGTTA CTTCCCTATG GGCCAGCTCT TATTGAGCAT 
TGTCTTATAG AAAATGGATT CTCTGGCAAT GTCAAAGTGG ACGAAAAACT TGAAAGCAAA 
GATATTGAGA AGATACTGGT TTGTGTGCAG AGGGCAGAAG ACTATTTGGA AAAAACAGCC 
AACTTCAATG GAAAGGGATA TATAATTCAG AAAAGAGAGG TAAAACCAAG CCTGGATGCC 
AATAAACCAG CTGAAGACAT ACTCACGTAT GAGGAATTTC ATCCTTTCTT GTTTTCTCAA 
CATTTACAAT GTCCATATAT AGAATTCGAA TCATTTGACA AGGCTGTGGA TGAATTTTAT 
TCCAAGATAG AAGGTCAGAA AATTGACTTA AAAGCATTGC AACAGGAAAA ACAAGCACTG 
AAGAAATTAG ATAATGTCCG AAAGGATCAT GAAAACAGAT TGGAAGCTCT TCAGCAGGCT 
CAGGAAATTG ACAAACTGAA AGGGGAGCTC ATAGAGATGA ACCTGCAGAT AGTTGACAGA 
GCCATTCAGG TAGTTCGAAG TGCATTAGCC AACCAGATAG ATTGGACAGA AATTGGAGTG 
ATTGTGAAAG AAGCACAAGC CCAGGGAGAC CCTGTTGCAA GTGCAATCAA AGAACTAAAA 
CTGCAAACAA ACCACATTAC CATGCTTCTA CGAAATCCAT ACTTGTTATC AGAGGAGGAA 
GATGGTGATG GTGATGGCAG CATTGAGAAC AGTGATGCTG AAGCACCAAA AGGGAAAAAA 
AAAAAGCAAA AGAACAAGCA GCTGCAGAAG CCTCAGAAGA ACAAGCCGCT GCTTGTCGAT 
GTGGACCTCA GCCTGTCAGC CTATGCCAAT GCCAAAAAGT ATTATGATCA TAAGAGGTAT 
GCTGCTAAAA AAACACAGAG AACTGTCGAA GCTGCTGAGA AGGCATTCAA ATCAGCAGAG 
AAGAAAACAA AGCAAACCTT AAAAGAAGTA CAAACAGTTA CTTCTATCCA AAAAGCAAGG 
AAAGTGTATT GGTTTGAGAA ATTTCTGTGG TTTATTAGTT CAGAGAACTA TCTCATTATA 
GGTGGTCGAG ATCAGCAACA GAATGAGATT ATTGTGAAAA GATACTTAAC ACCAGGAGAC 
ATTTATGTGC ATGCTGATCT TCATGGAGCT ACCAGCTGTG TAATTAAGAA TCCAACAGGA 
GATCCCATCC CTCCTCGGAC TTTGACTGAA GCAGGCACAA TGGCACTTTG CTACAGCGCA 
GCTTGGGATG CCCGTGTTAT CACGAGTGCT TGGTGGGTGC ACCATCATCA GGTCTCTAAA 
ACAGCACCGA CAGGAGAGTA CTTAACAACT GGAAGCTTCA TGATAAGAGG AAAAAAGAAT 
TTCCTTCCTC CTTCATACCT AATGATGGGG TTTAGCTTCC TTTTTAAGGT AGATGAGTCC 
TGTGTCTGGA GACATCGAGG TGAACGAAAA GTCAGAGTGC AGGATGAAGA CATGGAAACA 
TTGACAAGTT GCACAAGTGA ACTCATGTCA GAAGAAATGG AACAGCTAGA AGGGGGCGAC 
AGCAGTGAAG AAGAGACCGA GGAGTTACGT GGAACCACGG AAGAAGTGGA ACTCAGGACT 
CAGGCTGATC CAGAGGACAT TGCTGCTCAC AGTGGAAGAG AGGAACTGAG CTCTGAAGAT 
GGAGAAGCCA TCAAAACAGC CACGAAAGAT GAAGAGCCCA TTGGTGAGGT GAGGGAAGAG 
GAGGAAGAGC CCTTCGAATA TCCTGACACC ACCATTGACT TGTCCCATCT TCAGTCCCAA 
AGGCCCCTAC AGAAACTGAC TCCAAGAGAA GAATCTTTTA ATTCAAGTGA CAGTAAATCA 
CAGAGCCGAA GACATTTGTC AGCCAAGGAG AGAAGAGAAA TGAAAAAGAA AAAGCTCCCA 
TGTGACTCAG GGGATTTAGA AGTGATAGAA GAAAAGGACA AAGAAAAAGA AAGTGCTGTG 
CACAGTGAAG CTGACCAGAA CACAAGCAAA AATGTGGCAG CTGGACAGCC AATGAAAAGA 
GGCCAGAAGA GTAAAATGAA AAAAATGAAG GAAAAATACA AAGACCAGGA TGATGAAGAT 
CGTGAACTTA TTATGAAATT GTTGGCATCT GCAGGTTCAA ACAAAGAAGA AAAGGGGAAG 
AAAGGAAAGA AAGGAAAAAC AAAAGATGAA CCCGTGAAAA AAAACCCACA GAAACCCAGA 
GGTGGACAGC GGGTTTTAGA TGTTGTTAAA GAAACCCCGT CCCTTCAGGC GTCGACTCCT 
GACTTACAAG ACTTTGCTGT GGATGAGCCA CATGATGACA AGGAAGAACA TGATCTGGAT 
CAGCAGGGAA ATGAGGAAAA TCTATTTGAC TCTTTGACAG GGCAACCACA TCCTGAAGAT 
GTATTAATGT TTGCTATTCC AATATGTGCT CCTTACACCA TCATGACAAA CTATAAATAC 
AAAGTGAAAC TTACTCCTGG AGTTCAGAAA AAGGGAAAAG CTGCAAAGAC AGCCTTGAAC 
AGTTTCATGC ACTCCAAAGA AGCAACAGCA AGAGAAAAAG ACTTATTCCG AAGTGTGAAG 
GACACAGATT TATCAAGAAA CATTCCCGGG AAAGTGAAAG TGTCTGCCCC CAATCTTCTG 
CACGTAAAAA GAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A brain-derived MeCP2 complex supports a role for MeCP2 in RNA processing.
Bioscience reports31(5)333-43(2011 Oct)
Long SW,Ooi JY,Yau PM,Jones PL

Drosophila caliban, a nuclear export mediator, can function as a tumor suppressor in human lung cancer cells.
Oncogene24(56)8229-39(2005 Dec)
Bi X,Jones T,Abbasi F,Lee H,Stultz B,Hursh DA,Mortin MA

Gene and alternative splicing annotation with AIR.
Genome research15(1)54-66(2005 Jan)
Florea L,Di Francesco V,Miller J,Turner R,Yao A,Harris M,Walenz B,Mobarry C,Merkulov GV,Charlab R,Dew I,Deng Z,Istrail S,Li P,Sutton G

Expression pattern of mammalian cochlea outer hair cell (OHC) mRNA: screening of a rat OHC cDNA library.
DNA and cell biology18(1)1-10(1999 Jan)
Harter C,Ripoll C,Lenoir M,Hamel CP,Rebillard G