nt5e cDNA ORF clone, Pygocentrus nattereri(red-bellied piranha)

Gene
Clones
gRNAs

Summary

Gene Symbol	nt5e
Entrez Gene ID	108427502
Full Name	5'-nucleotidase ecto
Gene Type	protein-coding
Organism	Pygocentrus nattereri(red-bellied piranha)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017697709.1	XP_017553198.1	5'-nucleotidase

Pathways from BioSystems
Gene Ontology

The following nt5e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nt5e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPy30259	XM_017697709.1 Latest version!	Pygocentrus nattereri 5'-nucleotidase ecto (nt5e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nt5e ( XM_017697709.1 ) cDNA ORF clone, Pygocentrus nattereri(red-bellied piranha) -> XP_017553198.1 Pygocentrus nattereri 5'-nucleotidase ecto (nt5e), mRNA.

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CloneID	OPy30259
Clone ID Related Accession (Same CDS sequence)	XM_017697709.1
Accession Version	XM_017697709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-01
Organism	Pygocentrus nattereri(red-bellied piranha)
Product	5'-nucleotidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016243807.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pygocentrus nattereri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGGT GGCCGCGCAC GCTGCTCGCG CCGCTGCTGC TGCTGCTTTG TGTGCTAGCG 
CGCGCGGCGG ACTGGGAGCT CGCGCTGCTG CACACGAACG ATGTGCACGC GCGCGTCGAG 
GAGACCAACA AGGACTCGGG CAAGTGCACG AAGCCCCCCT GCTTCGCCGG CGTCGCCAGG 
AGGTTCACGA AGATCAAAGA GCTGCGGAGC GCGGAGAAGA ACGTGCTGCT GCTGGACGCG 
GGGGATCAGT TTCAGGGCAC CGTGTGGTTC AACTACTACA AAGGAGCCGA GGCGGCGCAC 
TTCATGAACA AGCTCGGCTA CGACGCTATG GCTCTGGGTA ACCACGAGTT TGATAATGGA 
GTGGATGGTC TGATTAAGCC CTTCCTTCAG GAGGTTAACT GCTCCGTGCT CAGCGCTAAC 
ATAAAGCCTG ATAGCACTCT CGAGGCAATG ATTAGCGGAC GTTACTTGGC CTCTAAAATC 
TTGACCGTCA ACTCTGAGAA GGTGGGCATA GTGGGATACA CCTCCAAGGA GACGCCCGCC 
CTGTCTCTCC CAGGACCACA CCTGCTTTTT GAAGATGAGA TCCCGGCCTT GCAGCGTGAG 
GTGGACAAAT TGAGCACTCT AGGTGTTAAC AAGATCATCG CTCTGGGCCA CTCTGGATTT 
GCTGTGGACA AGGAAATCGC CAAGAAGGTG CGGGGATTGG ACGTAGTCAT CGGAGGACAC 
ACCAACACAT TCTTATACAC AGGGACACCT CCATCAACAG AGGTGCCAGC TGGGCCCTAT 
CCATTAATGG TACAGTCAGA GGATGGGCGT CAGGTCCCAG TGGTGCAGGC CTATGCCTTT 
GGGAAATACC TCGGCTACCT CAAAGTGACC TTTGATTCGG CTGGAAACGT GATAAAGGCT 
GCAGGAAATC CCATTCTTCT TGATAGCTCA GTACCAGAGG ATCCAGACAT CAAGGCCGAG 
GTGGATTCCT GGAAGACAAA CTTGGCTAAT TACTCGTCTC AGTATGTGGG GAGAACACTG 
GTTTACCTCA ATGGAACCTT TGAGGAGTGC CGCTTCCGTG AATGTAACCT GGGGAACTTG 
GTCTGTGATG CCATGGTTCA TCACAACATC AAATATCCAG ATGAAGTCCA GTGGAACCAC 
GTGAGCGCCT GTATTTTGAA TGGAGGAGGG ATTCGAAGCC CCATTGATGA GCGTAACAGA 
AATGGTACCA TCACCATGGA GGACGTGATT GGTGTGCTGC CGTTCGGTGG GACATTTGAC 
CTGGTTCAGC TGAAGGGCTC CACTCTGTTT CAGGCCTTTG AGCACAGTGT GCACAGATAC 
GGAGGAAACA CTGGAGAGTT CCTGCAGGTC TCAGGGTTTC AGGTTGAGTA TGACCTGTCG 
AAATCCCCTG GCAAGCGAGT GAAGAACGTG AGGGTTTTAT GTACTGAGTG TCGGGTGCCT 
CGCTATGAGC CTTTGGACGC CAACAAGGTG TATAAAGTGG TAATGCCGTC TTATTTGGTG 
GACGGGGGCG ACGGATACAC CATGATCAAA CCGCAGATGC TCAAGCATGA CAGTGGTGAC 
CTGGACATAT CTGTGGTGTC CAGCTACATC TCAGAGAGGA AGAGAGTTCA CCCTGCGGTG 
GAGGGTCGTA TCCTCATCTT CAACTCTGCC ATCGGACAAC AAAGTCCCAC CACGGCTCTC 
TTGCTGTTTG TATTGCTATG GGCACTGTGT ATGAGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017553198.1
CDS	14..1735
Translation	MRRWPRTLLAPLLLLLCVLARAADWELALLHTNDVHARVEETNKDSGKCTKPPCFAGVARRFTKIKELRSAEKNVLLLDAGDQFQGTVWFNYYKGAEAAHFMNKLGYDAMALGNHEFDNGVDGLIKPFLQEVNCSVLSANIKPDSTLEAMISGRYLASKILTVNSEKVGIVGYTSKETPALSLPGPHLLFEDEIPALQREVDKLSTLGVNKIIALGHSGFAVDKEIAKKVRGLDVVIGGHTNTFLYTGTPPSTEVPAGPYPLMVQSEDGRQVPVVQAYAFGKYLGYLKVTFDSAGNVIKAAGNPILLDSSVPEDPDIKAEVDSWKTNLANYSSQYVGRTLVYLNGTFEECRFRECNLGNLVCDAMVHHNIKYPDEVQWNHVSACILNGGGIRSPIDERNRNGTITMEDVIGVLPFGGTFDLVQLKGSTLFQAFEHSVHRYGGNTGEFLQVSGFQVEYDLSKSPGKRVKNVRVLCTECRVPRYEPLDANKVYKVVMPSYLVDGGDGYTMIKPQMLKHDSGDLDISVVSSYISERKRVHPAVEGRILIFNSAIGQQSPTTALLLFVLLWALCMSQ

Target ORF information:

RefSeq Version	XM_017697709.1
Organism	Pygocentrus nattereri(red-bellied piranha)
Definition	Pygocentrus nattereri 5'-nucleotidase ecto (nt5e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017697709.1

ORF Insert Sequence:

ATGCGACGGT GGCCGCGCAC GCTGCTCGCG CCGCTGCTGC TGCTGCTTTG TGTGCTAGCG 
CGCGCGGCGG ACTGGGAGCT CGCGCTGCTG CACACGAACG ATGTGCACGC GCGCGTCGAG 
GAGACCAACA AGGACTCGGG CAAGTGCACG AAGCCCCCCT GCTTCGCCGG CGTCGCCAGG 
AGGTTCACGA AGATCAAAGA GCTGCGGAGC GCGGAGAAGA ACGTGCTGCT GCTGGACGCG 
GGGGATCAGT TTCAGGGCAC CGTGTGGTTC AACTACTACA AAGGAGCCGA GGCGGCGCAC 
TTCATGAACA AGCTCGGCTA CGACGCTATG GCTCTGGGTA ACCACGAGTT TGATAATGGA 
GTGGATGGTC TGATTAAGCC CTTCCTTCAG GAGGTTAACT GCTCCGTGCT CAGCGCTAAC 
ATAAAGCCTG ATAGCACTCT CGAGGCAATG ATTAGCGGAC GTTACTTGGC CTCTAAAATC 
TTGACCGTCA ACTCTGAGAA GGTGGGCATA GTGGGATACA CCTCCAAGGA GACGCCCGCC 
CTGTCTCTCC CAGGACCACA CCTGCTTTTT GAAGATGAGA TCCCGGCCTT GCAGCGTGAG 
GTGGACAAAT TGAGCACTCT AGGTGTTAAC AAGATCATCG CTCTGGGCCA CTCTGGATTT 
GCTGTGGACA AGGAAATCGC CAAGAAGGTG CGGGGATTGG ACGTAGTCAT CGGAGGACAC 
ACCAACACAT TCTTATACAC AGGGACACCT CCATCAACAG AGGTGCCAGC TGGGCCCTAT 
CCATTAATGG TACAGTCAGA GGATGGGCGT CAGGTCCCAG TGGTGCAGGC CTATGCCTTT 
GGGAAATACC TCGGCTACCT CAAAGTGACC TTTGATTCGG CTGGAAACGT GATAAAGGCT 
GCAGGAAATC CCATTCTTCT TGATAGCTCA GTACCAGAGG ATCCAGACAT CAAGGCCGAG 
GTGGATTCCT GGAAGACAAA CTTGGCTAAT TACTCGTCTC AGTATGTGGG GAGAACACTG 
GTTTACCTCA ATGGAACCTT TGAGGAGTGC CGCTTCCGTG AATGTAACCT GGGGAACTTG 
GTCTGTGATG CCATGGTTCA TCACAACATC AAATATCCAG ATGAAGTCCA GTGGAACCAC 
GTGAGCGCCT GTATTTTGAA TGGAGGAGGG ATTCGAAGCC CCATTGATGA GCGTAACAGA 
AATGGTACCA TCACCATGGA GGACGTGATT GGTGTGCTGC CGTTCGGTGG GACATTTGAC 
CTGGTTCAGC TGAAGGGCTC CACTCTGTTT CAGGCCTTTG AGCACAGTGT GCACAGATAC 
GGAGGAAACA CTGGAGAGTT CCTGCAGGTC TCAGGGTTTC AGGTTGAGTA TGACCTGTCG 
AAATCCCCTG GCAAGCGAGT GAAGAACGTG AGGGTTTTAT GTACTGAGTG TCGGGTGCCT 
CGCTATGAGC CTTTGGACGC CAACAAGGTG TATAAAGTGG TAATGCCGTC TTATTTGGTG 
GACGGGGGCG ACGGATACAC CATGATCAAA CCGCAGATGC TCAAGCATGA CAGTGGTGAC 
CTGGACATAT CTGTGGTGTC CAGCTACATC TCAGAGAGGA AGAGAGTTCA CCCTGCGGTG 
GAGGGTCGTA TCCTCATCTT CAACTCTGCC ATCGGACAAC AAAGTCCCAC CACGGCTCTC 
TTGCTGTTTG TATTGCTATG GGCACTGTGT ATGAGCCAGT GA