ECE1 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECE1
Entrez Gene ID	105290656
Full Name	endothelin converting enzyme 1
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011358021.1	XP_011356323.1	endothelin-converting enzyme 1
XM_023529394.1	XP_023385162.1	endothelin-converting enzyme 1

Pathways from BioSystems
Gene Ontology

The following ECE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt45235	XM_011358021.1 Latest version!	Pteropus vampyrus endothelin converting enzyme 1 (ECE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPt68668	XM_023529394.1 Latest version!	Pteropus vampyrus endothelin converting enzyme 1 (ECE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECE1 ( XM_011358021.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011356323.1 Pteropus vampyrus endothelin converting enzyme 1 (ECE1), mRNA.

Price & Availability↑

CloneID	OPt45235
Clone ID Related Accession (Same CDS sequence)	XM_011358021.1
Accession Version	XM_011358021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-22
Organism	Pteropus vampyrus(large flying fox)
Product	endothelin-converting enzyme 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888817.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 11% of CDS bases ##RefSeq-Attributes-END##

ATGGAAACAC TGAGGGAGTC CGTGCTACAC TTGGCCTTGC AGATGTCGAC CTACAAGCGT 
GCCACGCTGG ACGAGGAGGA CCTGGTGGAC TCGCTCTCCG AGGGCGACGG GTACCCAAAT 
GGTCTGCAGG TGAACTTCCG CAGCCCCCGG AGTGGCCAGA GGTGCTGGGC TGCACGGACC 
CAGGTGGAGA AGCGGCTGGT GGTGCTGGTG GCACTTCTGG CAGCAGGGCT GGTGGCCTGC 
TTCACAGCTC TGGGCATCCA GTATCAGACA AGAACCCCCT CAGTGTGCCT AAGCCAGGCC 
TGTGTCTCAG TGACCAGCTC CATCTTGAAT TCCATGGACC CCACGGTGGA CCCCTGCCAG 
GACTTCTTCA CCTATGCCTG TGGTGGATGG ATCAAAGCCA ACCCTGTGCC TGATGGTCAC 
TCACGTTGGG GGACCTTCAG CAACCTCTGG GAGCATAATC AAGCCATCAT CAAGCACCTC 
CTCGAAAACT CCACGGCCAG TGTGAGTGAA GCAGAAAAGA AGGCCCAAGT ATACTACCGT 
GCGTGTATGA ACGAAACCAG GATCGAGGAG CTCAAGGCCA AGCCCCTGAT GGAGCTGATA 
GAGAGGCTCG GGGGCTGGAA CATCACAGGG CCCTGGGACA AAGACAATTT CCAGGACATC 
CTGCAGGTGG TCACCTCCCA CTACCGCACC TCACCCTTCT TCTCCGTCTA CGTCAGTGCA 
GACTCCAAGA ACTCCAACAG CAACGTGATC CAGGTGGACC AGTCTGGCCT GGGTTTACCC 
TCACGGGACT ATTACCTGAA CAAAACTGAA AATGAGAAGG TACTGACAGG ATACCTGAAC 
TACATGATCC AGCTGGGGAA GCTGCTGGGC GGAGGGGACG AGGATGCCGT CCGGCCCCAG 
ATGCAGCAGA TCCTGGACTT TGAGACAGCT CTGGCCAACA TCACCATCCC CCAGGAGAAG 
CGCCGGGACG AGGAGCTCAT CTACCACAAA GTGACAGCGG CCGAGCTGCA GACCTTGGCC 
CCCGCCATCA ACTGGCTGCC CTTCCTCAAC ACCATCTTCT ACCCCGTGGA GATCAACGAA 
TCGGAGCCTA TTGTTGTCTA TGACAAGGAG TACCTCGGGC AGGTCTCCAC CCTCATCAAC 
AGCACCGACA AATGCCTGCT GAACAACTAC ATGATCTGGA ACCTGGTGCG AAAGACAAGC 
ACCTTCCTCG ATCAGCGCTT TCAAGACGCC GATGAGAAGT TTATGGAAGT CATGTATGGG 
ACCAAGAAGA CCTGTCTTCC TCGCTGGAAG TTTTGCGTGA GTGACACAGA AAACAACTTG 
GGCTTCGCCC TGGGCCCCAT GTTTGTCAAA GAGACCTTCG CCGAAGACAG CAAGAACATA 
GCCAGTGAGA TAATCCTGGA GATCAAGAAG GCGTTTGAGG AAAGCCTGAG CACCCTGAAG 
TGGATGGATG AAGACACTCG GAAATCAGCC AAAGAAAAGG CAGATGCAAT CTACAACATG 
ATAGGCTACC CCAACTTCAT CATGGACCCC AAGGAGCTGG ACAAAGTGTT CAATGACTAC 
ACTGCAGTTC CGGACCTCTA CTTCGAGAAT GCCATGCGGT TTTTCAACTT CTCCTGGAGG 
GTCACCGCGG ACCAGCTCAG GAAAGCTCCC AACAGAGACC AGTGGAGCAT GACCCCCCCC 
ATGGTGAACG CTTACTACTC ACCCACCAAG AACGAGATTG TGTTTCCGGC CGGAATCCTG 
CAGGCCCCAT TCTACACCCG CTCTTCACCC AAGGCCTTAA ATTTTGGTGG CATCGGTGTC 
GTCGTGGGCC ACGAACTGAC TCATGCTTTT GATGATCAAG GTGTCCAGCC CTTGGGGGTG 
GGTGGTAGTC AGCAGGAGCA TATTCCTCAC CACCACTCGA GAATTCTCTG GTCTGACCAC 
AATCAGAGGA GTCGAAGTCA GTCCCCTGAT CACCAGCTAA GGTCCAGAAT CCTCATTGCC 
TCCTGCCTTG GAGAGCCCAA GTCCGAGGAG GAGGAGGAGG GAAGTTCTAC CCTGTCGAGA 
TCTACCGAGT TCTCCCGTGC TAGCCTCTCC GTCGCCACCC TGCCTGTCCC TTGTCACGGG 
GGCCCTGAGA TCCGTCTTCT CCCCTCCCAG GCCTACCAGA ACTGGGTGAA GAAGAATGGG 
GCCGAGCAGA CATTGCCCAC CCTGGGTCTC ACCAATAACC AGCTCTTCTT CCTGGGGTTC 
GCGCAGGTCT GGTGCTCCGT CCGCACGCCT GAGAGCACCC ACGAAGGCCT CATCACTGAT 
CCCCACAGCC CCTCCCGCTT CCGGGTCATC GGCTCTGTCT CCAACTCGAA GGAGTTCCCC 
GAACACTTCC ACTGCCCGCC CGGCTCACCC ATGAACCCGC GTCACAAATG TGAAGTCTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011356323.1
CDS	1..2403
Translation	METLRESVLHLALQMSTYKRATLDEEDLVDSLSEGDGYPNGLQVNFRSPRSGQRCWAARTQVEKRLVVLVALLAAGLVACFTALGIQYQTRTPSVCLSQACVSVTSSILNSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAEKKAQVYYRACMNETRIEELKAKPLMELIERLGGWNITGPWDKDNFQDILQVVTSHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMIQLGKLLGGGDEDAVRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLGQVSTLINSTDKCLLNNYMIWNLVRKTSTFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKETFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGVQPLGVGGSQQEHIPHHHSRILWSDHNQRSRSQSPDHQLRSRILIASCLGEPKSEEEEEGSSTLSRSTEFSRASLSVATLPVPCHGGPEIRLLPSQAYQNWVKKNGAEQTLPTLGLTNNQLFFLGFAQVWCSVRTPESTHEGLITDPHSPSRFRVIGSVSNSKEFPEHFHCPPGSPMNPRHKCEVW

Target ORF information:

RefSeq Version	XM_011358021.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus endothelin converting enzyme 1 (ECE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011358021.1

ORF Insert Sequence:

ATGGAAACAC TGAGGGAGTC CGTGCTACAC TTGGCCTTGC AGATGTCGAC CTACAAGCGT 
GCCACGCTGG ACGAGGAGGA CCTGGTGGAC TCGCTCTCCG AGGGCGACGG GTACCCAAAT 
GGTCTGCAGG TGAACTTCCG CAGCCCCCGG AGTGGCCAGA GGTGCTGGGC TGCACGGACC 
CAGGTGGAGA AGCGGCTGGT GGTGCTGGTG GCACTTCTGG CAGCAGGGCT GGTGGCCTGC 
TTCACAGCTC TGGGCATCCA GTATCAGACA AGAACCCCCT CAGTGTGCCT AAGCCAGGCC 
TGTGTCTCAG TGACCAGCTC CATCTTGAAT TCCATGGACC CCACGGTGGA CCCCTGCCAG 
GACTTCTTCA CCTATGCCTG TGGTGGATGG ATCAAAGCCA ACCCTGTGCC TGATGGTCAC 
TCACGTTGGG GGACCTTCAG CAACCTCTGG GAGCATAATC AAGCCATCAT CAAGCACCTC 
CTCGAAAACT CCACGGCCAG TGTGAGTGAA GCAGAAAAGA AGGCCCAAGT ATACTACCGT 
GCGTGTATGA ACGAAACCAG GATCGAGGAG CTCAAGGCCA AGCCCCTGAT GGAGCTGATA 
GAGAGGCTCG GGGGCTGGAA CATCACAGGG CCCTGGGACA AAGACAATTT CCAGGACATC 
CTGCAGGTGG TCACCTCCCA CTACCGCACC TCACCCTTCT TCTCCGTCTA CGTCAGTGCA 
GACTCCAAGA ACTCCAACAG CAACGTGATC CAGGTGGACC AGTCTGGCCT GGGTTTACCC 
TCACGGGACT ATTACCTGAA CAAAACTGAA AATGAGAAGG TACTGACAGG ATACCTGAAC 
TACATGATCC AGCTGGGGAA GCTGCTGGGC GGAGGGGACG AGGATGCCGT CCGGCCCCAG 
ATGCAGCAGA TCCTGGACTT TGAGACAGCT CTGGCCAACA TCACCATCCC CCAGGAGAAG 
CGCCGGGACG AGGAGCTCAT CTACCACAAA GTGACAGCGG CCGAGCTGCA GACCTTGGCC 
CCCGCCATCA ACTGGCTGCC CTTCCTCAAC ACCATCTTCT ACCCCGTGGA GATCAACGAA 
TCGGAGCCTA TTGTTGTCTA TGACAAGGAG TACCTCGGGC AGGTCTCCAC CCTCATCAAC 
AGCACCGACA AATGCCTGCT GAACAACTAC ATGATCTGGA ACCTGGTGCG AAAGACAAGC 
ACCTTCCTCG ATCAGCGCTT TCAAGACGCC GATGAGAAGT TTATGGAAGT CATGTATGGG 
ACCAAGAAGA CCTGTCTTCC TCGCTGGAAG TTTTGCGTGA GTGACACAGA AAACAACTTG 
GGCTTCGCCC TGGGCCCCAT GTTTGTCAAA GAGACCTTCG CCGAAGACAG CAAGAACATA 
GCCAGTGAGA TAATCCTGGA GATCAAGAAG GCGTTTGAGG AAAGCCTGAG CACCCTGAAG 
TGGATGGATG AAGACACTCG GAAATCAGCC AAAGAAAAGG CAGATGCAAT CTACAACATG 
ATAGGCTACC CCAACTTCAT CATGGACCCC AAGGAGCTGG ACAAAGTGTT CAATGACTAC 
ACTGCAGTTC CGGACCTCTA CTTCGAGAAT GCCATGCGGT TTTTCAACTT CTCCTGGAGG 
GTCACCGCGG ACCAGCTCAG GAAAGCTCCC AACAGAGACC AGTGGAGCAT GACCCCCCCC 
ATGGTGAACG CTTACTACTC ACCCACCAAG AACGAGATTG TGTTTCCGGC CGGAATCCTG 
CAGGCCCCAT TCTACACCCG CTCTTCACCC AAGGCCTTAA ATTTTGGTGG CATCGGTGTC 
GTCGTGGGCC ACGAACTGAC TCATGCTTTT GATGATCAAG GTGTCCAGCC CTTGGGGGTG 
GGTGGTAGTC AGCAGGAGCA TATTCCTCAC CACCACTCGA GAATTCTCTG GTCTGACCAC 
AATCAGAGGA GTCGAAGTCA GTCCCCTGAT CACCAGCTAA GGTCCAGAAT CCTCATTGCC 
TCCTGCCTTG GAGAGCCCAA GTCCGAGGAG GAGGAGGAGG GAAGTTCTAC CCTGTCGAGA 
TCTACCGAGT TCTCCCGTGC TAGCCTCTCC GTCGCCACCC TGCCTGTCCC TTGTCACGGG 
GGCCCTGAGA TCCGTCTTCT CCCCTCCCAG GCCTACCAGA ACTGGGTGAA GAAGAATGGG 
GCCGAGCAGA CATTGCCCAC CCTGGGTCTC ACCAATAACC AGCTCTTCTT CCTGGGGTTC 
GCGCAGGTCT GGTGCTCCGT CCGCACGCCT GAGAGCACCC ACGAAGGCCT CATCACTGAT 
CCCCACAGCC CCTCCCGCTT CCGGGTCATC GGCTCTGTCT CCAACTCGAA GGAGTTCCCC 
GAACACTTCC ACTGCCCGCC CGGCTCACCC ATGAACCCGC GTCACAAATG TGAAGTCTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_023529394.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023385162.1 Pteropus vampyrus endothelin converting enzyme 1 (ECE1), mRNA.

Price & Availability↑

CloneID	OPt68668
Clone ID Related Accession (Same CDS sequence)	XM_023529394.1
Accession Version	XM_023529394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-18
Organism	Pteropus vampyrus(large flying fox)
Product	endothelin-converting enzyme 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888817.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCCCCGC CCCTGGGATG GGCTTCCTAC CTGATGAGAC CCAGCCCCTG CCTTCAAGGT 
TCTCCTCCTG GAGTGCTGTG TGCTTCCATA GAAGGAAGCG TGAGTGGGGG ACGCAGCAGG 
GAACCAGACA CCACCTGGAG TCAGGAAGGT GGTCAGTGGA TCTTCCAAGA AGGGCTGACC 
TCTGAAAAGA TACGAAAGAG GAAGAGCCAC TTCTTCACTG TACCTGGGCA AGTTACTTCA 
CTGACCCTCA GTTTCAGGCT CTGTGAAAGG GGCACCCGCT TCAGAGAGAA GATGTCGACC 
TACAAGCGTG CCACGCTGGA CGAGGAGGAC CTGGTGGACT CGCTCTCCGA GGGCGACGGG 
TACCCAAATG GTCTGCAGGT GAACTTCCGC AGCCCCCGGA GTGGCCAGAG GTGCTGGGCT 
GCACGGACCC AGGTGGAGAA GCGGCTGGTG GTGCTGGTGG CACTTCTGGC AGCAGGGCTG 
GTGGCCTGCT TCACAGCTCT GGGCATCCAG TATCAGACAA GAACCCCCTC AGTGTGCCTA 
AGCCAGGCCT GTGTCTCAGT GACCAGCTCC ATCTTGAATT CCATGGACCC CACGGTGGAC 
CCCTGCCAGG ACTTCTTCAC CTATGCCTGT GGTGGATGGA TCAAAGCCAA CCCTGTGCCT 
GATGGTCACT CACGTTGGGG GACCTTCAGC AACCTCTGGG AGCATAATCA AGCCATCATC 
AAGCACCTCC TCGAAAACTC CACGGCCAGT GTGAGTGAAG CAGAAAAGAA GGCCCAAGTA 
TACTACCGTG CGTGTATGAA CGAAACCAGG ATCGAGGAGC TCAAGGCCAA GCCCCTGATG 
GAGCTGATAG AGAGGCTCGG GGGCTGGAAC ATCACAGGGC CCTGGGACAA AGACAATTTC 
CAGGACATCC TGCAGGTGGT CACCTCCCAC TACCGCACCT CACCCTTCTT CTCCGTCTAC 
GTCAGTGCAG ACTCCAAGAA CTCCAACAGC AACGTGATCC AGGTGGACCA GTCTGGCCTG 
GGTTTACCCT CACGGGACTA TTACCTGAAC AAAACTGAAA ATGAGAAGGT ACTGACAGGA 
TACCTGAACT ACATGATCCA GCTGGGGAAG CTGCTGGGCG GAGGGGACGA GGATGCCGTC 
CGGCCCCAGA TGCAGCAGAT CCTGGACTTT GAGACAGCTC TGGCCAACAT CACCATCCCC 
CAGGAGAAGC GCCGGGACGA GGAGCTCATC TACCACAAAG TGACAGCGGC CGAGCTGCAG 
ACCTTGGCCC CCGCCATCAA CTGGCTGCCC TTCCTCAACA CCATCTTCTA CCCCGTGGAG 
ATCAACGAAT CGGAGCCTAT TGTTGTCTAT GACAAGGAGT ACCTCGGGCA GGTCTCCACC 
CTCATCAACA GCACCGACAA ATGCCTGCTG AACAACTACA TGATCTGGAA CCTGGTGCGA 
AAGACAAGCA CCTTCCTCGA TCAGCGCTTT CAAGACGCCG ATGAGAAGTT TATGGAAGTC 
ATGTATGGGA CCAAGAAGAC CTGTCTTCCT CGCTGGAAGT TTTGCGTGAG TGACACAGAA 
AACAACTTGG GCTTCGCCCT GGGCCCCATG TTTGTCAAAG AGACCTTCGC CGAAGACAGC 
AAGAACATAG CCAGTGAGAT AATCCTGGAG ATCAAGAAGG CGTTTGAGGA AAGCCTGAGC 
ACCCTGAAGT GGATGGATGA AGACACTCGG AAATCAGCCA AAGAAAAGGC AGATGCAATC 
TACAACATGA TAGGCTACCC CAACTTCATC ATGGACCCCA AGGAGCTGGA CAAAGTGTTC 
AATGACTACA CTGCAGTTCC GGACCTCTAC TTCGAGAATG CCATGCGGTT TTTCAACTTC 
TCCTGGAGGG TCACCGCGGA CCAGCTCAGG AAAGCTCCCA ACAGAGACCA GTGGAGCATG 
ACCCCCCCCA TGGTGAACGC TTACTACTCA CCCACCAAGA ACGAGATTGT GTTTCCGGCC 
GGAATCCTGC AGGCCCCATT CTACACCCGC TCTTCACCCA AGGCCTTAAA TTTTGGTGGC 
ATCGGTGTCG TCGTGGGCCA CGAACTGACT CATGCTTTTG ATGATCAAGG TATGGGTTCC 
TTGGGGGTCC CCGTTAGATG GGCCCACTCG CTTCCTGGAG CCGCTGATCA TCTGCTTTTG 
CTCCCATCAC TTCTGAGAGG AAGTTCTACC CTGTCGAGAT CTACCGAGTT CTCCCGTGCT 
AGCCTCTCCG TCGCCACCCT GCCTGTCCCT TGTCACGGGG GCCCTGAGAT CCGTCTTCTC 
CCCTCCCAGG CCTACCAGAA CTGGGTGAAG AAGAATGGGG CCGAGCAGAC ATTGCCCACC 
CTGGGTCTCA CCAATAACCA GCTCTTCTTC CTGGGGTTCG CGCAGGTCTG GTGCTCCGTC 
CGCACGCCTG AGAGCACCCA CGAAGGCCTC ATCACTGATC CCCACAGCCC CTCCCGCTTC 
CGGGTCATCG GCTCTGTCTC CAACTCGAAG GAGTTCCCCG AACACTTCCA CTGCCCGCCC 
GGCTCACCCA TGAACCCGCG TCACAAATGT GAAGTCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023385162.1
CDS	16..2577
Translation	MAPPLGWASYLMRPSPCLQGSPPGVLCASIEGSVSGGRSREPDTTWSQEGGQWIFQEGLTSEKIRKRKSHFFTVPGQVTSLTLSFRLCERGTRFREKMSTYKRATLDEEDLVDSLSEGDGYPNGLQVNFRSPRSGQRCWAARTQVEKRLVVLVALLAAGLVACFTALGIQYQTRTPSVCLSQACVSVTSSILNSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAEKKAQVYYRACMNETRIEELKAKPLMELIERLGGWNITGPWDKDNFQDILQVVTSHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMIQLGKLLGGGDEDAVRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLGQVSTLINSTDKCLLNNYMIWNLVRKTSTFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKETFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGMGSLGVPVRWAHSLPGAADHLLLLPSLLRGSSTLSRSTEFSRASLSVATLPVPCHGGPEIRLLPSQAYQNWVKKNGAEQTLPTLGLTNNQLFFLGFAQVWCSVRTPESTHEGLITDPHSPSRFRVIGSVSNSKEFPEHFHCPPGSPMNPRHKCEVW

Target ORF information:

RefSeq Version	XM_023529394.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus endothelin converting enzyme 1 (ECE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023529394.1

ORF Insert Sequence:

ATGGCCCCGC CCCTGGGATG GGCTTCCTAC CTGATGAGAC CCAGCCCCTG CCTTCAAGGT 
TCTCCTCCTG GAGTGCTGTG TGCTTCCATA GAAGGAAGCG TGAGTGGGGG ACGCAGCAGG 
GAACCAGACA CCACCTGGAG TCAGGAAGGT GGTCAGTGGA TCTTCCAAGA AGGGCTGACC 
TCTGAAAAGA TACGAAAGAG GAAGAGCCAC TTCTTCACTG TACCTGGGCA AGTTACTTCA 
CTGACCCTCA GTTTCAGGCT CTGTGAAAGG GGCACCCGCT TCAGAGAGAA GATGTCGACC 
TACAAGCGTG CCACGCTGGA CGAGGAGGAC CTGGTGGACT CGCTCTCCGA GGGCGACGGG 
TACCCAAATG GTCTGCAGGT GAACTTCCGC AGCCCCCGGA GTGGCCAGAG GTGCTGGGCT 
GCACGGACCC AGGTGGAGAA GCGGCTGGTG GTGCTGGTGG CACTTCTGGC AGCAGGGCTG 
GTGGCCTGCT TCACAGCTCT GGGCATCCAG TATCAGACAA GAACCCCCTC AGTGTGCCTA 
AGCCAGGCCT GTGTCTCAGT GACCAGCTCC ATCTTGAATT CCATGGACCC CACGGTGGAC 
CCCTGCCAGG ACTTCTTCAC CTATGCCTGT GGTGGATGGA TCAAAGCCAA CCCTGTGCCT 
GATGGTCACT CACGTTGGGG GACCTTCAGC AACCTCTGGG AGCATAATCA AGCCATCATC 
AAGCACCTCC TCGAAAACTC CACGGCCAGT GTGAGTGAAG CAGAAAAGAA GGCCCAAGTA 
TACTACCGTG CGTGTATGAA CGAAACCAGG ATCGAGGAGC TCAAGGCCAA GCCCCTGATG 
GAGCTGATAG AGAGGCTCGG GGGCTGGAAC ATCACAGGGC CCTGGGACAA AGACAATTTC 
CAGGACATCC TGCAGGTGGT CACCTCCCAC TACCGCACCT CACCCTTCTT CTCCGTCTAC 
GTCAGTGCAG ACTCCAAGAA CTCCAACAGC AACGTGATCC AGGTGGACCA GTCTGGCCTG 
GGTTTACCCT CACGGGACTA TTACCTGAAC AAAACTGAAA ATGAGAAGGT ACTGACAGGA 
TACCTGAACT ACATGATCCA GCTGGGGAAG CTGCTGGGCG GAGGGGACGA GGATGCCGTC 
CGGCCCCAGA TGCAGCAGAT CCTGGACTTT GAGACAGCTC TGGCCAACAT CACCATCCCC 
CAGGAGAAGC GCCGGGACGA GGAGCTCATC TACCACAAAG TGACAGCGGC CGAGCTGCAG 
ACCTTGGCCC CCGCCATCAA CTGGCTGCCC TTCCTCAACA CCATCTTCTA CCCCGTGGAG 
ATCAACGAAT CGGAGCCTAT TGTTGTCTAT GACAAGGAGT ACCTCGGGCA GGTCTCCACC 
CTCATCAACA GCACCGACAA ATGCCTGCTG AACAACTACA TGATCTGGAA CCTGGTGCGA 
AAGACAAGCA CCTTCCTCGA TCAGCGCTTT CAAGACGCCG ATGAGAAGTT TATGGAAGTC 
ATGTATGGGA CCAAGAAGAC CTGTCTTCCT CGCTGGAAGT TTTGCGTGAG TGACACAGAA 
AACAACTTGG GCTTCGCCCT GGGCCCCATG TTTGTCAAAG AGACCTTCGC CGAAGACAGC 
AAGAACATAG CCAGTGAGAT AATCCTGGAG ATCAAGAAGG CGTTTGAGGA AAGCCTGAGC 
ACCCTGAAGT GGATGGATGA AGACACTCGG AAATCAGCCA AAGAAAAGGC AGATGCAATC 
TACAACATGA TAGGCTACCC CAACTTCATC ATGGACCCCA AGGAGCTGGA CAAAGTGTTC 
AATGACTACA CTGCAGTTCC GGACCTCTAC TTCGAGAATG CCATGCGGTT TTTCAACTTC 
TCCTGGAGGG TCACCGCGGA CCAGCTCAGG AAAGCTCCCA ACAGAGACCA GTGGAGCATG 
ACCCCCCCCA TGGTGAACGC TTACTACTCA CCCACCAAGA ACGAGATTGT GTTTCCGGCC 
GGAATCCTGC AGGCCCCATT CTACACCCGC TCTTCACCCA AGGCCTTAAA TTTTGGTGGC 
ATCGGTGTCG TCGTGGGCCA CGAACTGACT CATGCTTTTG ATGATCAAGG TATGGGTTCC 
TTGGGGGTCC CCGTTAGATG GGCCCACTCG CTTCCTGGAG CCGCTGATCA TCTGCTTTTG 
CTCCCATCAC TTCTGAGAGG AAGTTCTACC CTGTCGAGAT CTACCGAGTT CTCCCGTGCT 
AGCCTCTCCG TCGCCACCCT GCCTGTCCCT TGTCACGGGG GCCCTGAGAT CCGTCTTCTC 
CCCTCCCAGG CCTACCAGAA CTGGGTGAAG AAGAATGGGG CCGAGCAGAC ATTGCCCACC 
CTGGGTCTCA CCAATAACCA GCTCTTCTTC CTGGGGTTCG CGCAGGTCTG GTGCTCCGTC 
CGCACGCCTG AGAGCACCCA CGAAGGCCTC ATCACTGATC CCCACAGCCC CTCCCGCTTC 
CGGGTCATCG GCTCTGTCTC CAACTCGAAG GAGTTCCCCG AACACTTCCA CTGCCCGCCC 
GGCTCACCCA TGAACCCGCG TCACAAATGT GAAGTCTGGT GA