UNC13D cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	UNC13D
Entrez Gene ID	102897275
Full Name	unc-13 homolog D
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015589776.1	XP_015445262.1	protein unc-13 homolog D isoform X1
XM_015589778.1	XP_015445264.1	protein unc-13 homolog D isoform X3
XM_006912213.2	XP_006912275.1	protein unc-13 homolog D isoform X2
XM_015589776.2	XP_015445262.1	protein unc-13 homolog D isoform X1
XM_015589778.2	XP_015445264.1	protein unc-13 homolog D isoform X3
XM_015589777.1	XP_015445263.1	protein unc-13 homolog D isoform X2
XM_015589777.2	XP_015445263.1	protein unc-13 homolog D isoform X2

Pathways from BioSystems
Gene Ontology

The following UNC13D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UNC13D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt15190	XM_015589776.2 Latest version!	Pteropus alecto unc-13 homolog D (UNC13D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPt15191	XM_006912213.2 Latest version!	Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPt15191	XM_015589777.2 Latest version!	Pteropus alecto unc-13 homolog D (UNC13D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPt15192	XM_015589778.2 Latest version!	Pteropus alecto unc-13 homolog D (UNC13D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OPt15190	XM_015589776.1	Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPt15192	XM_015589778.1	Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPt15191	XM_015589777.1	Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UNC13D ( XM_015589776.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015445262.1 Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPt15190
Clone ID Related Accession (Same CDS sequence)	XM_015589776.1 , XM_015589776.2
Accession Version	XM_015589776.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	protein unc-13 homolog D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006436283.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCCG GACCTGTCCC TCTCCCCACC AGCCCTCCTC CCCCCCTCCG CTGGCACAAG 
CGGCGCCCGC CCCTCCTGCG CCAGGCCATC AAGATAAGGC GCCGCAGGGT CAGAGACCTG 
CAGGGTCCCC TGCCCCACAC TGCTCAGGAG ATCGAGGCTC CATCCCACCA CTTCTCCCCT 
GAGGAGCGGG CCCTGCTCTA CGAGGAAGCC CTCTACACTG TCCTGCACCG CCTGGGAAAG 
CCCGAGCCCG GCCATGTGAG CGAGGCGTCC GAGCTGCTGC GCTACCTGCA GGAGGCCTTC 
CACGTGGAGC CCGAGGAACA CCAGCGGATG CTGCGGCAGG TGCAGGCCCT CGAGAAGCCA 
ATATTTTGTC TGAAGGCAAC GGTGAAACAA GCCAAGGGCA TTCTGGGCAA GGATGTCAGC 
GGGTTCAGTG ACCCCTACTG CCTGCTGGGC ATTGAGCAGG GAGTGGGCAC GCTGGAGGGC 
AGCCCCGGGT CTCGGCGGCG GCAGAAGGCT GTGGTGAAGC ACACCATCCC GGAGGAGCAG 
ACACACCGCA CCCAGGTCAT CAGCCAGACA CTCAACCCCG TCTGGGACGA GACCTTCATC 
CTGGAGTTTG AGGACATAAG AAATTCGAGT TTTCATCTGG ACATGTGGGA CCTGGACACC 
GTGGAGTCTG TCAGGCAGAA GCTTGGGGAG CTCACAGATC TGCACGGGCT GCGCAGGATC 
TTTAAGGAGG CCCGGAAGGA CAAAGGCCAG GACGACTTCC TGGGAAACGT GGTGCTGCGG 
CTGCAGGACC TTCGCTGCCG GGAGGATCAG TGGTACCCTC TGGAGCCCTG CACTGAGACC 
TACCCAGACC GTGGCCAGTG CCACCTCCAG TTCCAGCTCA TCCACAAGCG GAGAGCCACT 
GGGGCCAGCC GCTCCCAGCC CAGCTACACG GTGCACCTCC ACCTGCTGCA ACAGCTCGTG 
TCCCACGAGG TCACCCAGCA CCAGGCAGGC AGCACCTCCT GGGATGGGCT GCTGAGTCCT 
CAGGCTGCCA CCATCCTGTT TCTCCATGCC ACGCAGAAGG ACCTGTCCGA CTTCCACCAG 
TCCATGGCGC AGTGGCTGGC CTACAGCCGT GTCTACCAGA GCCTGGAGTT CCCCAGCAGC 
TGCCTCCTGC ACCCTATCAC CAGCATCGAG TACCAGTGGA TCCAGGGCCG GCTCAAGGCA 
GAGCAGATGG AGGAGCTGGC AACCTCATTC AGCTCCCTGC TGGCATATGG CCTCTCCCTC 
ATCCGGAGGT TCCGGTCTGT CTTCCCCTTA TCTGTCTCGG ACTCCCCAGC TCGGCTACAG 
TCTCTCCTCA GGGTCCTGGT ACAGATGTGC AAGATGAAGG CCTTTAGAGA ACTGTGCCCT 
GACAGTGGCC CACTGCCCCA CCTGGTGACT GAGGCGCTGC GGGCTGGCAC CGTTGAATGG 
TTCCACCTGA AGCAGCAGCA CCATCAGCCA ATGGTGCAGG GCATGCTGGA GGCAGGCAAG 
GCCTTGCTGA GCCTGGTCCA AGACATCATC GGTGACCTGC ACCAGTGCCA GCGCACGTGG 
AACAAGATCT TCCACAATGC CCTTAAGATC GACCTCTTCG CCATGGCTTT CCTGGAGCTG 
CAGTGGCTGG TGGCCAAGCG CGTGCAGGAC CACACGGCCG CGGCGTTGAG AGGCCTCGTG 
TCCCCAGAGA TGGGAGAGAG TTTGTTCCAG CTCTACATCA TCCTGAAGGA GCTCTGCCAG 
CTGGGCCCCG CCCCCTCGGA GAGGGATGGA GTCCTGGCCC TGGATGGCTT CCACCGCTGG 
TTCCAGCCTG CCATCCCTTC CTGGCTGCAG AAGACATACA GCGTGGCCTT GGCGCGGGTG 
CAGCGCGCTG TGCAGATGGA CAAGCTGGTG CCCCTGGGTG AACTGACCAA GCATAGCACA 
TCGGCCGTGG ATCTGTCCAC CTGCTTTGCT CAGATCAGCC ACACTGCCCG GCAGCTGGAC 
TGGCCCGACC CAGAGGAGGC CTTCATGATC ACAGTCAAGT TTGTGGAGGA CACCTGTCGG 
CTGGCCCTGG TGTACTGCAG CCTGATAAAG GCCCGGGCCC GAGAGCTCTC TGCAGGCCAG 
AAGGACCAGG GCCAGGCAGC CAATATGCTG TGCGTGGTGG TGAACGACAT GGAGCAGCTG 
CGGCTGGTGA TCGGCAGGCT GCCCACCCAG CTGGCATGGG AGGCGCTGGA GCAGCGAGTA 
GGGGCTGTGC TGGAGCAGGG GCAGCTGCAG AACACGCTGC ATGCCCAGCT GCAGGGTGCG 
CTGGCGGGGC TGGGCCACGA GATCCGCACC GGCGTCCGCA CCCTTGCGGA GCAGCTGGAG 
GTGGGCATCG CCAAGCACAT CCAGAACCTC GTGGGCGTCA GGGAGTCCAT CTTGCCTGAG 
GATGCCATTC TGCCCCTGAT GAAATTCCTG GAGGTGGAGC TCTGCTACAT GAACACCAAC 
TTGGTGCAGG AGAACTTCAG CAGCCTTCTG ACCCTGCTCT GGACCCACAC GCTCGAGGTG 
ATGGTTGAGG GTGCCACCTC CCAGCGGAGC TGCCCCCTGG CCTCTAGCAG GCTGAAGATT 
GCGCTGCAGA ACCTGGAGAT CTGCTTCTAC GCAGAGGGCT GTGGCCTGCA GCCCGAGGCC 
CTGCACACGG CCACCTTCCA GGCTTTGCAG AGGGACTTAG AGCTGCAGGC GGCCTCCAGC 
CGGGAGCTCA TCCAGAAGTA CTTCTGCAAC CGCATCCAGC AGCAGGCGGA AACCACCTCT 
GAGGAGCTCG GGGCGATCAC CGTCAAGGCC TCCTACCGCG CCTCTGAGCA GAAGCTGCAT 
GTGGAGGTGC TCAGCGCCTC CAGCCTGCTG CCCCTGGACT CCAATGGCTC CAGCGACCCC 
TTTGTTCAGC TGACCTTGGA GCCCAGGCAC GAGTTTCCTG AGCTGGCCCC CCGGGAAACC 
CAAAAGCACA AGAAGGACCT TCACCCGCTG TTTGATGAGA CCTTTGAATT CCTGGTGCCT 
GCTGAGCCGT GCCAGAAGGA CGGGGCGTGC CTCCTGCTCA CAGTGCTGGA CCATGACACA 
TTGGGGGCCG ACGACCTGGA GGGCGAGGCC TTTCTGCCGC TGCGCTCGGT GCTGGGCCTC 
AGTGGGACGG AGGAGCCTGG GGAGGTGCCT CAGAGCCGCC TGCCCCTCAC CTACCCCGCA 
CCCAATGGGG ACCCAATCCT GCAGCTTCTG GAGAGCCGGA AGGGTGATCG TGAGGCCCAG 
GTGTTTGTGA AGCTGCGGCG GCAGCGAGCC AAGCAGGCCT CCCAGCATGC CGTGCGGCTG 
GGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015445262.1
CDS	10..3318
Translation	MKAGPVPLPTSPPPPLRWHKRRPPLLRQAIKIRRRRVRDLQGPLPHTAQEIEAPSHHFSPEERALLYEEALYTVLHRLGKPEPGHVSEASELLRYLQEAFHVEPEEHQRMLRQVQALEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGTLEGSPGSRRRQKAVVKHTIPEEQTHRTQVISQTLNPVWDETFILEFEDIRNSSFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPCTETYPDRGQCHLQFQLIHKRRATGASRSQPSYTVHLHLLQQLVSHEVTQHQAGSTSWDGLLSPQAATILFLHATQKDLSDFHQSMAQWLAYSRVYQSLEFPSSCLLHPITSIEYQWIQGRLKAEQMEELATSFSSLLAYGLSLIRRFRSVFPLSVSDSPARLQSLLRVLVQMCKMKAFRELCPDSGPLPHLVTEALRAGTVEWFHLKQQHHQPMVQGMLEAGKALLSLVQDIIGDLHQCQRTWNKIFHNALKIDLFAMAFLELQWLVAKRVQDHTAAALRGLVSPEMGESLFQLYIILKELCQLGPAPSERDGVLALDGFHRWFQPAIPSWLQKTYSVALARVQRAVQMDKLVPLGELTKHSTSAVDLSTCFAQISHTARQLDWPDPEEAFMITVKFVEDTCRLALVYCSLIKARARELSAGQKDQGQAANMLCVVVNDMEQLRLVIGRLPTQLAWEALEQRVGAVLEQGQLQNTLHAQLQGALAGLGHEIRTGVRTLAEQLEVGIAKHIQNLVGVRESILPEDAILPLMKFLEVELCYMNTNLVQENFSSLLTLLWTHTLEVMVEGATSQRSCPLASSRLKIALQNLEICFYAEGCGLQPEALHTATFQALQRDLELQAASSRELIQKYFCNRIQQQAETTSEELGAITVKASYRASEQKLHVEVLSASSLLPLDSNGSSDPFVQLTLEPRHEFPELAPRETQKHKKDLHPLFDETFEFLVPAEPCQKDGACLLLTVLDHDTLGADDLEGEAFLPLRSVLGLSGTEEPGEVPQSRLPLTYPAPNGDPILQLLESRKGDREAQVFVKLRRQRAKQASQHAVRLGQ

Target ORF information:

RefSeq Version	XM_015589776.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015589776.1

ORF Insert Sequence:

ATGAAAGCCG GACCTGTCCC TCTCCCCACC AGCCCTCCTC CCCCCCTCCG CTGGCACAAG 
CGGCGCCCGC CCCTCCTGCG CCAGGCCATC AAGATAAGGC GCCGCAGGGT CAGAGACCTG 
CAGGGTCCCC TGCCCCACAC TGCTCAGGAG ATCGAGGCTC CATCCCACCA CTTCTCCCCT 
GAGGAGCGGG CCCTGCTCTA CGAGGAAGCC CTCTACACTG TCCTGCACCG CCTGGGAAAG 
CCCGAGCCCG GCCATGTGAG CGAGGCGTCC GAGCTGCTGC GCTACCTGCA GGAGGCCTTC 
CACGTGGAGC CCGAGGAACA CCAGCGGATG CTGCGGCAGG TGCAGGCCCT CGAGAAGCCA 
ATATTTTGTC TGAAGGCAAC GGTGAAACAA GCCAAGGGCA TTCTGGGCAA GGATGTCAGC 
GGGTTCAGTG ACCCCTACTG CCTGCTGGGC ATTGAGCAGG GAGTGGGCAC GCTGGAGGGC 
AGCCCCGGGT CTCGGCGGCG GCAGAAGGCT GTGGTGAAGC ACACCATCCC GGAGGAGCAG 
ACACACCGCA CCCAGGTCAT CAGCCAGACA CTCAACCCCG TCTGGGACGA GACCTTCATC 
CTGGAGTTTG AGGACATAAG AAATTCGAGT TTTCATCTGG ACATGTGGGA CCTGGACACC 
GTGGAGTCTG TCAGGCAGAA GCTTGGGGAG CTCACAGATC TGCACGGGCT GCGCAGGATC 
TTTAAGGAGG CCCGGAAGGA CAAAGGCCAG GACGACTTCC TGGGAAACGT GGTGCTGCGG 
CTGCAGGACC TTCGCTGCCG GGAGGATCAG TGGTACCCTC TGGAGCCCTG CACTGAGACC 
TACCCAGACC GTGGCCAGTG CCACCTCCAG TTCCAGCTCA TCCACAAGCG GAGAGCCACT 
GGGGCCAGCC GCTCCCAGCC CAGCTACACG GTGCACCTCC ACCTGCTGCA ACAGCTCGTG 
TCCCACGAGG TCACCCAGCA CCAGGCAGGC AGCACCTCCT GGGATGGGCT GCTGAGTCCT 
CAGGCTGCCA CCATCCTGTT TCTCCATGCC ACGCAGAAGG ACCTGTCCGA CTTCCACCAG 
TCCATGGCGC AGTGGCTGGC CTACAGCCGT GTCTACCAGA GCCTGGAGTT CCCCAGCAGC 
TGCCTCCTGC ACCCTATCAC CAGCATCGAG TACCAGTGGA TCCAGGGCCG GCTCAAGGCA 
GAGCAGATGG AGGAGCTGGC AACCTCATTC AGCTCCCTGC TGGCATATGG CCTCTCCCTC 
ATCCGGAGGT TCCGGTCTGT CTTCCCCTTA TCTGTCTCGG ACTCCCCAGC TCGGCTACAG 
TCTCTCCTCA GGGTCCTGGT ACAGATGTGC AAGATGAAGG CCTTTAGAGA ACTGTGCCCT 
GACAGTGGCC CACTGCCCCA CCTGGTGACT GAGGCGCTGC GGGCTGGCAC CGTTGAATGG 
TTCCACCTGA AGCAGCAGCA CCATCAGCCA ATGGTGCAGG GCATGCTGGA GGCAGGCAAG 
GCCTTGCTGA GCCTGGTCCA AGACATCATC GGTGACCTGC ACCAGTGCCA GCGCACGTGG 
AACAAGATCT TCCACAATGC CCTTAAGATC GACCTCTTCG CCATGGCTTT CCTGGAGCTG 
CAGTGGCTGG TGGCCAAGCG CGTGCAGGAC CACACGGCCG CGGCGTTGAG AGGCCTCGTG 
TCCCCAGAGA TGGGAGAGAG TTTGTTCCAG CTCTACATCA TCCTGAAGGA GCTCTGCCAG 
CTGGGCCCCG CCCCCTCGGA GAGGGATGGA GTCCTGGCCC TGGATGGCTT CCACCGCTGG 
TTCCAGCCTG CCATCCCTTC CTGGCTGCAG AAGACATACA GCGTGGCCTT GGCGCGGGTG 
CAGCGCGCTG TGCAGATGGA CAAGCTGGTG CCCCTGGGTG AACTGACCAA GCATAGCACA 
TCGGCCGTGG ATCTGTCCAC CTGCTTTGCT CAGATCAGCC ACACTGCCCG GCAGCTGGAC 
TGGCCCGACC CAGAGGAGGC CTTCATGATC ACAGTCAAGT TTGTGGAGGA CACCTGTCGG 
CTGGCCCTGG TGTACTGCAG CCTGATAAAG GCCCGGGCCC GAGAGCTCTC TGCAGGCCAG 
AAGGACCAGG GCCAGGCAGC CAATATGCTG TGCGTGGTGG TGAACGACAT GGAGCAGCTG 
CGGCTGGTGA TCGGCAGGCT GCCCACCCAG CTGGCATGGG AGGCGCTGGA GCAGCGAGTA 
GGGGCTGTGC TGGAGCAGGG GCAGCTGCAG AACACGCTGC ATGCCCAGCT GCAGGGTGCG 
CTGGCGGGGC TGGGCCACGA GATCCGCACC GGCGTCCGCA CCCTTGCGGA GCAGCTGGAG 
GTGGGCATCG CCAAGCACAT CCAGAACCTC GTGGGCGTCA GGGAGTCCAT CTTGCCTGAG 
GATGCCATTC TGCCCCTGAT GAAATTCCTG GAGGTGGAGC TCTGCTACAT GAACACCAAC 
TTGGTGCAGG AGAACTTCAG CAGCCTTCTG ACCCTGCTCT GGACCCACAC GCTCGAGGTG 
ATGGTTGAGG GTGCCACCTC CCAGCGGAGC TGCCCCCTGG CCTCTAGCAG GCTGAAGATT 
GCGCTGCAGA ACCTGGAGAT CTGCTTCTAC GCAGAGGGCT GTGGCCTGCA GCCCGAGGCC 
CTGCACACGG CCACCTTCCA GGCTTTGCAG AGGGACTTAG AGCTGCAGGC GGCCTCCAGC 
CGGGAGCTCA TCCAGAAGTA CTTCTGCAAC CGCATCCAGC AGCAGGCGGA AACCACCTCT 
GAGGAGCTCG GGGCGATCAC CGTCAAGGCC TCCTACCGCG CCTCTGAGCA GAAGCTGCAT 
GTGGAGGTGC TCAGCGCCTC CAGCCTGCTG CCCCTGGACT CCAATGGCTC CAGCGACCCC 
TTTGTTCAGC TGACCTTGGA GCCCAGGCAC GAGTTTCCTG AGCTGGCCCC CCGGGAAACC 
CAAAAGCACA AGAAGGACCT TCACCCGCTG TTTGATGAGA CCTTTGAATT CCTGGTGCCT 
GCTGAGCCGT GCCAGAAGGA CGGGGCGTGC CTCCTGCTCA CAGTGCTGGA CCATGACACA 
TTGGGGGCCG ACGACCTGGA GGGCGAGGCC TTTCTGCCGC TGCGCTCGGT GCTGGGCCTC 
AGTGGGACGG AGGAGCCTGG GGAGGTGCCT CAGAGCCGCC TGCCCCTCAC CTACCCCGCA 
CCCAATGGGG ACCCAATCCT GCAGCTTCTG GAGAGCCGGA AGGGTGATCG TGAGGCCCAG 
GTGTTTGTGA AGCTGCGGCG GCAGCGAGCC AAGCAGGCCT CCCAGCATGC CGTGCGGCTG 
GGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13D ( XM_015589776.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015445262.1 Pteropus alecto unc-13 homolog D (UNC13D), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPt15190
Clone ID Related Accession (Same CDS sequence)	XM_015589776.1 , XM_015589776.2
Accession Version	XM_015589776.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	protein unc-13 homolog D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006436283.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015589776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCCG GACCTGTCCC TCTCCCCACC AGCCCTCCTC CCCCCCTCCG CTGGCACAAG 
CGGCGCCCGC CCCTCCTGCG CCAGGCCATC AAGATAAGGC GCCGCAGGGT CAGAGACCTG 
CAGGGTCCCC TGCCCCACAC TGCTCAGGAG ATCGAGGCTC CATCCCACCA CTTCTCCCCT 
GAGGAGCGGG CCCTGCTCTA CGAGGAAGCC CTCTACACTG TCCTGCACCG CCTGGGAAAG 
CCCGAGCCCG GCCATGTGAG CGAGGCGTCC GAGCTGCTGC GCTACCTGCA GGAGGCCTTC 
CACGTGGAGC CCGAGGAACA CCAGCGGATG CTGCGGCAGG TGCAGGCCCT CGAGAAGCCA 
ATATTTTGTC TGAAGGCAAC GGTGAAACAA GCCAAGGGCA TTCTGGGCAA GGATGTCAGC 
GGGTTCAGTG ACCCCTACTG CCTGCTGGGC ATTGAGCAGG GAGTGGGCAC GCTGGAGGGC 
AGCCCCGGGT CTCGGCGGCG GCAGAAGGCT GTGGTGAAGC ACACCATCCC GGAGGAGCAG 
ACACACCGCA CCCAGGTCAT CAGCCAGACA CTCAACCCCG TCTGGGACGA GACCTTCATC 
CTGGAGTTTG AGGACATAAG AAATTCGAGT TTTCATCTGG ACATGTGGGA CCTGGACACC 
GTGGAGTCTG TCAGGCAGAA GCTTGGGGAG CTCACAGATC TGCACGGGCT GCGCAGGATC 
TTTAAGGAGG CCCGGAAGGA CAAAGGCCAG GACGACTTCC TGGGAAACGT GGTGCTGCGG 
CTGCAGGACC TTCGCTGCCG GGAGGATCAG TGGTACCCTC TGGAGCCCTG CACTGAGACC 
TACCCAGACC GTGGCCAGTG CCACCTCCAG TTCCAGCTCA TCCACAAGCG GAGAGCCACT 
GGGGCCAGCC GCTCCCAGCC CAGCTACACG GTGCACCTCC ACCTGCTGCA ACAGCTCGTG 
TCCCACGAGG TCACCCAGCA CCAGGCAGGC AGCACCTCCT GGGATGGGCT GCTGAGTCCT 
CAGGCTGCCA CCATCCTGTT TCTCCATGCC ACGCAGAAGG ACCTGTCCGA CTTCCACCAG 
TCCATGGCGC AGTGGCTGGC CTACAGCCGT GTCTACCAGA GCCTGGAGTT CCCCAGCAGC 
TGCCTCCTGC ACCCTATCAC CAGCATCGAG TACCAGTGGA TCCAGGGCCG GCTCAAGGCA 
GAGCAGATGG AGGAGCTGGC AACCTCATTC AGCTCCCTGC TGGCATATGG CCTCTCCCTC 
ATCCGGAGGT TCCGGTCTGT CTTCCCCTTA TCTGTCTCGG ACTCCCCAGC TCGGCTACAG 
TCTCTCCTCA GGGTCCTGGT ACAGATGTGC AAGATGAAGG CCTTTAGAGA ACTGTGCCCT 
GACAGTGGCC CACTGCCCCA CCTGGTGACT GAGGCGCTGC GGGCTGGCAC CGTTGAATGG 
TTCCACCTGA AGCAGCAGCA CCATCAGCCA ATGGTGCAGG GCATGCTGGA GGCAGGCAAG 
GCCTTGCTGA GCCTGGTCCA AGACATCATC GGTGACCTGC ACCAGTGCCA GCGCACGTGG 
AACAAGATCT TCCACAATGC CCTTAAGATC GACCTCTTCG CCATGGCTTT CCTGGAGCTG 
CAGTGGCTGG TGGCCAAGCG CGTGCAGGAC CACACGGCCG CGGCGTTGAG AGGCCTCGTG 
TCCCCAGAGA TGGGAGAGAG TTTGTTCCAG CTCTACATCA TCCTGAAGGA GCTCTGCCAG 
CTGGGCCCCG CCCCCTCGGA GAGGGATGGA GTCCTGGCCC TGGATGGCTT CCACCGCTGG 
TTCCAGCCTG CCATCCCTTC CTGGCTGCAG AAGACATACA GCGTGGCCTT GGCGCGGGTG 
CAGCGCGCTG TGCAGATGGA CAAGCTGGTG CCCCTGGGTG AACTGACCAA GCATAGCACA 
TCGGCCGTGG ATCTGTCCAC CTGCTTTGCT CAGATCAGCC ACACTGCCCG GCAGCTGGAC 
TGGCCCGACC CAGAGGAGGC CTTCATGATC ACAGTCAAGT TTGTGGAGGA CACCTGTCGG 
CTGGCCCTGG TGTACTGCAG CCTGATAAAG GCCCGGGCCC GAGAGCTCTC TGCAGGCCAG 
AAGGACCAGG GCCAGGCAGC CAATATGCTG TGCGTGGTGG TGAACGACAT GGAGCAGCTG 
CGGCTGGTGA TCGGCAGGCT GCCCACCCAG CTGGCATGGG AGGCGCTGGA GCAGCGAGTA 
GGGGCTGTGC TGGAGCAGGG GCAGCTGCAG AACACGCTGC ATGCCCAGCT GCAGGGTGCG 
CTGGCGGGGC TGGGCCACGA GATCCGCACC GGCGTCCGCA CCCTTGCGGA GCAGCTGGAG 
GTGGGCATCG CCAAGCACAT CCAGAACCTC GTGGGCGTCA GGGAGTCCAT CTTGCCTGAG 
GATGCCATTC TGCCCCTGAT GAAATTCCTG GAGGTGGAGC TCTGCTACAT GAACACCAAC 
TTGGTGCAGG AGAACTTCAG CAGCCTTCTG ACCCTGCTCT GGACCCACAC GCTCGAGGTG 
ATGGTTGAGG GTGCCACCTC CCAGCGGAGC TGCCCCCTGG CCTCTAGCAG GCTGAAGATT 
GCGCTGCAGA ACCTGGAGAT CTGCTTCTAC GCAGAGGGCT GTGGCCTGCA GCCCGAGGCC 
CTGCACACGG CCACCTTCCA GGCTTTGCAG AGGGACTTAG AGCTGCAGGC GGCCTCCAGC 
CGGGAGCTCA TCCAGAAGTA CTTCTGCAAC CGCATCCAGC AGCAGGCGGA AACCACCTCT 
GAGGAGCTCG GGGCGATCAC CGTCAAGGCC TCCTACCGCG CCTCTGAGCA GAAGCTGCAT 
GTGGAGGTGC TCAGCGCCTC CAGCCTGCTG CCCCTGGACT CCAATGGCTC CAGCGACCCC 
TTTGTTCAGC TGACCTTGGA GCCCAGGCAC GAGTTTCCTG AGCTGGCCCC CCGGGAAACC 
CAAAAGCACA AGAAGGACCT TCACCCGCTG TTTGATGAGA CCTTTGAATT CCTGGTGCCT 
GCTGAGCCGT GCCAGAAGGA CGGGGCGTGC CTCCTGCTCA CAGTGCTGGA CCATGACACA 
TTGGGGGCCG ACGACCTGGA GGGCGAGGCC TTTCTGCCGC TGCGCTCGGT GCTGGGCCTC 
AGTGGGACGG AGGAGCCTGG GGAGGTGCCT CAGAGCCGCC TGCCCCTCAC CTACCCCGCA 
CCCAATGGGG ACCCAATCCT GCAGCTTCTG GAGAGCCGGA AGGGTGATCG TGAGGCCCAG 
GTGTTTGTGA AGCTGCGGCG GCAGCGAGCC AAGCAGGCCT CCCAGCATGC CGTGCGGCTG 
GGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015445262.1
CDS	75..3383
Translation	MKAGPVPLPTSPPPPLRWHKRRPPLLRQAIKIRRRRVRDLQGPLPHTAQEIEAPSHHFSPEERALLYEEALYTVLHRLGKPEPGHVSEASELLRYLQEAFHVEPEEHQRMLRQVQALEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGTLEGSPGSRRRQKAVVKHTIPEEQTHRTQVISQTLNPVWDETFILEFEDIRNSSFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPCTETYPDRGQCHLQFQLIHKRRATGASRSQPSYTVHLHLLQQLVSHEVTQHQAGSTSWDGLLSPQAATILFLHATQKDLSDFHQSMAQWLAYSRVYQSLEFPSSCLLHPITSIEYQWIQGRLKAEQMEELATSFSSLLAYGLSLIRRFRSVFPLSVSDSPARLQSLLRVLVQMCKMKAFRELCPDSGPLPHLVTEALRAGTVEWFHLKQQHHQPMVQGMLEAGKALLSLVQDIIGDLHQCQRTWNKIFHNALKIDLFAMAFLELQWLVAKRVQDHTAAALRGLVSPEMGESLFQLYIILKELCQLGPAPSERDGVLALDGFHRWFQPAIPSWLQKTYSVALARVQRAVQMDKLVPLGELTKHSTSAVDLSTCFAQISHTARQLDWPDPEEAFMITVKFVEDTCRLALVYCSLIKARARELSAGQKDQGQAANMLCVVVNDMEQLRLVIGRLPTQLAWEALEQRVGAVLEQGQLQNTLHAQLQGALAGLGHEIRTGVRTLAEQLEVGIAKHIQNLVGVRESILPEDAILPLMKFLEVELCYMNTNLVQENFSSLLTLLWTHTLEVMVEGATSQRSCPLASSRLKIALQNLEICFYAEGCGLQPEALHTATFQALQRDLELQAASSRELIQKYFCNRIQQQAETTSEELGAITVKASYRASEQKLHVEVLSASSLLPLDSNGSSDPFVQLTLEPRHEFPELAPRETQKHKKDLHPLFDETFEFLVPAEPCQKDGACLLLTVLDHDTLGADDLEGEAFLPLRSVLGLSGTEEPGEVPQSRLPLTYPAPNGDPILQLLESRKGDREAQVFVKLRRQRAKQASQHAVRLGQ

Target ORF information:

RefSeq Version	XM_015589776.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto unc-13 homolog D (UNC13D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015589776.2

ORF Insert Sequence:

ATGAAAGCCG GACCTGTCCC TCTCCCCACC AGCCCTCCTC CCCCCCTCCG CTGGCACAAG 
CGGCGCCCGC CCCTCCTGCG CCAGGCCATC AAGATAAGGC GCCGCAGGGT CAGAGACCTG 
CAGGGTCCCC TGCCCCACAC TGCTCAGGAG ATCGAGGCTC CATCCCACCA CTTCTCCCCT 
GAGGAGCGGG CCCTGCTCTA CGAGGAAGCC CTCTACACTG TCCTGCACCG CCTGGGAAAG 
CCCGAGCCCG GCCATGTGAG CGAGGCGTCC GAGCTGCTGC GCTACCTGCA GGAGGCCTTC 
CACGTGGAGC CCGAGGAACA CCAGCGGATG CTGCGGCAGG TGCAGGCCCT CGAGAAGCCA 
ATATTTTGTC TGAAGGCAAC GGTGAAACAA GCCAAGGGCA TTCTGGGCAA GGATGTCAGC 
GGGTTCAGTG ACCCCTACTG CCTGCTGGGC ATTGAGCAGG GAGTGGGCAC GCTGGAGGGC 
AGCCCCGGGT CTCGGCGGCG GCAGAAGGCT GTGGTGAAGC ACACCATCCC GGAGGAGCAG 
ACACACCGCA CCCAGGTCAT CAGCCAGACA CTCAACCCCG TCTGGGACGA GACCTTCATC 
CTGGAGTTTG AGGACATAAG AAATTCGAGT TTTCATCTGG ACATGTGGGA CCTGGACACC 
GTGGAGTCTG TCAGGCAGAA GCTTGGGGAG CTCACAGATC TGCACGGGCT GCGCAGGATC 
TTTAAGGAGG CCCGGAAGGA CAAAGGCCAG GACGACTTCC TGGGAAACGT GGTGCTGCGG 
CTGCAGGACC TTCGCTGCCG GGAGGATCAG TGGTACCCTC TGGAGCCCTG CACTGAGACC 
TACCCAGACC GTGGCCAGTG CCACCTCCAG TTCCAGCTCA TCCACAAGCG GAGAGCCACT 
GGGGCCAGCC GCTCCCAGCC CAGCTACACG GTGCACCTCC ACCTGCTGCA ACAGCTCGTG 
TCCCACGAGG TCACCCAGCA CCAGGCAGGC AGCACCTCCT GGGATGGGCT GCTGAGTCCT 
CAGGCTGCCA CCATCCTGTT TCTCCATGCC ACGCAGAAGG ACCTGTCCGA CTTCCACCAG 
TCCATGGCGC AGTGGCTGGC CTACAGCCGT GTCTACCAGA GCCTGGAGTT CCCCAGCAGC 
TGCCTCCTGC ACCCTATCAC CAGCATCGAG TACCAGTGGA TCCAGGGCCG GCTCAAGGCA 
GAGCAGATGG AGGAGCTGGC AACCTCATTC AGCTCCCTGC TGGCATATGG CCTCTCCCTC 
ATCCGGAGGT TCCGGTCTGT CTTCCCCTTA TCTGTCTCGG ACTCCCCAGC TCGGCTACAG 
TCTCTCCTCA GGGTCCTGGT ACAGATGTGC AAGATGAAGG CCTTTAGAGA ACTGTGCCCT 
GACAGTGGCC CACTGCCCCA CCTGGTGACT GAGGCGCTGC GGGCTGGCAC CGTTGAATGG 
TTCCACCTGA AGCAGCAGCA CCATCAGCCA ATGGTGCAGG GCATGCTGGA GGCAGGCAAG 
GCCTTGCTGA GCCTGGTCCA AGACATCATC GGTGACCTGC ACCAGTGCCA GCGCACGTGG 
AACAAGATCT TCCACAATGC CCTTAAGATC GACCTCTTCG CCATGGCTTT CCTGGAGCTG 
CAGTGGCTGG TGGCCAAGCG CGTGCAGGAC CACACGGCCG CGGCGTTGAG AGGCCTCGTG 
TCCCCAGAGA TGGGAGAGAG TTTGTTCCAG CTCTACATCA TCCTGAAGGA GCTCTGCCAG 
CTGGGCCCCG CCCCCTCGGA GAGGGATGGA GTCCTGGCCC TGGATGGCTT CCACCGCTGG 
TTCCAGCCTG CCATCCCTTC CTGGCTGCAG AAGACATACA GCGTGGCCTT GGCGCGGGTG 
CAGCGCGCTG TGCAGATGGA CAAGCTGGTG CCCCTGGGTG AACTGACCAA GCATAGCACA 
TCGGCCGTGG ATCTGTCCAC CTGCTTTGCT CAGATCAGCC ACACTGCCCG GCAGCTGGAC 
TGGCCCGACC CAGAGGAGGC CTTCATGATC ACAGTCAAGT TTGTGGAGGA CACCTGTCGG 
CTGGCCCTGG TGTACTGCAG CCTGATAAAG GCCCGGGCCC GAGAGCTCTC TGCAGGCCAG 
AAGGACCAGG GCCAGGCAGC CAATATGCTG TGCGTGGTGG TGAACGACAT GGAGCAGCTG 
CGGCTGGTGA TCGGCAGGCT GCCCACCCAG CTGGCATGGG AGGCGCTGGA GCAGCGAGTA 
GGGGCTGTGC TGGAGCAGGG GCAGCTGCAG AACACGCTGC ATGCCCAGCT GCAGGGTGCG 
CTGGCGGGGC TGGGCCACGA GATCCGCACC GGCGTCCGCA CCCTTGCGGA GCAGCTGGAG 
GTGGGCATCG CCAAGCACAT CCAGAACCTC GTGGGCGTCA GGGAGTCCAT CTTGCCTGAG 
GATGCCATTC TGCCCCTGAT GAAATTCCTG GAGGTGGAGC TCTGCTACAT GAACACCAAC 
TTGGTGCAGG AGAACTTCAG CAGCCTTCTG ACCCTGCTCT GGACCCACAC GCTCGAGGTG 
ATGGTTGAGG GTGCCACCTC CCAGCGGAGC TGCCCCCTGG CCTCTAGCAG GCTGAAGATT 
GCGCTGCAGA ACCTGGAGAT CTGCTTCTAC GCAGAGGGCT GTGGCCTGCA GCCCGAGGCC 
CTGCACACGG CCACCTTCCA GGCTTTGCAG AGGGACTTAG AGCTGCAGGC GGCCTCCAGC 
CGGGAGCTCA TCCAGAAGTA CTTCTGCAAC CGCATCCAGC AGCAGGCGGA AACCACCTCT 
GAGGAGCTCG GGGCGATCAC CGTCAAGGCC TCCTACCGCG CCTCTGAGCA GAAGCTGCAT 
GTGGAGGTGC TCAGCGCCTC CAGCCTGCTG CCCCTGGACT CCAATGGCTC CAGCGACCCC 
TTTGTTCAGC TGACCTTGGA GCCCAGGCAC GAGTTTCCTG AGCTGGCCCC CCGGGAAACC 
CAAAAGCACA AGAAGGACCT TCACCCGCTG TTTGATGAGA CCTTTGAATT CCTGGTGCCT 
GCTGAGCCGT GCCAGAAGGA CGGGGCGTGC CTCCTGCTCA CAGTGCTGGA CCATGACACA 
TTGGGGGCCG ACGACCTGGA GGGCGAGGCC TTTCTGCCGC TGCGCTCGGT GCTGGGCCTC 
AGTGGGACGG AGGAGCCTGG GGAGGTGCCT CAGAGCCGCC TGCCCCTCAC CTACCCCGCA 
CCCAATGGGG ACCCAATCCT GCAGCTTCTG GAGAGCCGGA AGGGTGATCG TGAGGCCCAG 
GTGTTTGTGA AGCTGCGGCG GCAGCGAGCC AAGCAGGCCT CCCAGCATGC CGTGCGGCTG 
GGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13D ( XM_006912213.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006912275.1 Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X2, mRNA.

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CloneID	OPt15191
Clone ID Related Accession (Same CDS sequence)	XM_006912213.2 , XM_015589777.1 , XM_015589777.2
Accession Version	XM_006912213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	protein unc-13 homolog D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006436283.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006912213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACAC CCCTCTCTCA CTCACAGCGG CGCCCGCCCC TCCTGCGCCA GGCCATCAAG 
ATAAGGCGCC GCAGGGTCAG AGACCTGCAG GGTCCCCTGC CCCACACTGC TCAGGAGATC 
GAGGCTCCAT CCCACCACTT CTCCCCTGAG GAGCGGGCCC TGCTCTACGA GGAAGCCCTC 
TACACTGTCC TGCACCGCCT GGGAAAGCCC GAGCCCGGCC ATGTGAGCGA GGCGTCCGAG 
CTGCTGCGCT ACCTGCAGGA GGCCTTCCAC GTGGAGCCCG AGGAACACCA GCGGATGCTG 
CGGCAGGTGC AGGCCCTCGA GAAGCCAATA TTTTGTCTGA AGGCAACGGT GAAACAAGCC 
AAGGGCATTC TGGGCAAGGA TGTCAGCGGG TTCAGTGACC CCTACTGCCT GCTGGGCATT 
GAGCAGGGAG TGGGCACGCT GGAGGGCAGC CCCGGGTCTC GGCGGCGGCA GAAGGCTGTG 
GTGAAGCACA CCATCCCGGA GGAGCAGACA CACCGCACCC AGGTCATCAG CCAGACACTC 
AACCCCGTCT GGGACGAGAC CTTCATCCTG GAGTTTGAGG ACATAAGAAA TTCGAGTTTT 
CATCTGGACA TGTGGGACCT GGACACCGTG GAGTCTGTCA GGCAGAAGCT TGGGGAGCTC 
ACAGATCTGC ACGGGCTGCG CAGGATCTTT AAGGAGGCCC GGAAGGACAA AGGCCAGGAC 
GACTTCCTGG GAAACGTGGT GCTGCGGCTG CAGGACCTTC GCTGCCGGGA GGATCAGTGG 
TACCCTCTGG AGCCCTGCAC TGAGACCTAC CCAGACCGTG GCCAGTGCCA CCTCCAGTTC 
CAGCTCATCC ACAAGCGGAG AGCCACTGGG GCCAGCCGCT CCCAGCCCAG CTACACGGTG 
CACCTCCACC TGCTGCAACA GCTCGTGTCC CACGAGGTCA CCCAGCACCA GGCAGGCAGC 
ACCTCCTGGG ATGGGCTGCT GAGTCCTCAG GCTGCCACCA TCCTGTTTCT CCATGCCACG 
CAGAAGGACC TGTCCGACTT CCACCAGTCC ATGGCGCAGT GGCTGGCCTA CAGCCGTGTC 
TACCAGAGCC TGGAGTTCCC CAGCAGCTGC CTCCTGCACC CTATCACCAG CATCGAGTAC 
CAGTGGATCC AGGGCCGGCT CAAGGCAGAG CAGATGGAGG AGCTGGCAAC CTCATTCAGC 
TCCCTGCTGG CATATGGCCT CTCCCTCATC CGGAGGTTCC GGTCTGTCTT CCCCTTATCT 
GTCTCGGACT CCCCAGCTCG GCTACAGTCT CTCCTCAGGG TCCTGGTACA GATGTGCAAG 
ATGAAGGCCT TTAGAGAACT GTGCCCTGAC AGTGGCCCAC TGCCCCACCT GGTGACTGAG 
GCGCTGCGGG CTGGCACCGT TGAATGGTTC CACCTGAAGC AGCAGCACCA TCAGCCAATG 
GTGCAGGGCA TGCTGGAGGC AGGCAAGGCC TTGCTGAGCC TGGTCCAAGA CATCATCGGT 
GACCTGCACC AGTGCCAGCG CACGTGGAAC AAGATCTTCC ACAATGCCCT TAAGATCGAC 
CTCTTCGCCA TGGCTTTCCT GGAGCTGCAG TGGCTGGTGG CCAAGCGCGT GCAGGACCAC 
ACGGCCGCGG CGTTGAGAGG CCTCGTGTCC CCAGAGATGG GAGAGAGTTT GTTCCAGCTC 
TACATCATCC TGAAGGAGCT CTGCCAGCTG GGCCCCGCCC CCTCGGAGAG GGATGGAGTC 
CTGGCCCTGG ATGGCTTCCA CCGCTGGTTC CAGCCTGCCA TCCCTTCCTG GCTGCAGAAG 
ACATACAGCG TGGCCTTGGC GCGGGTGCAG CGCGCTGTGC AGATGGACAA GCTGGTGCCC 
CTGGGTGAAC TGACCAAGCA TAGCACATCG GCCGTGGATC TGTCCACCTG CTTTGCTCAG 
ATCAGCCACA CTGCCCGGCA GCTGGACTGG CCCGACCCAG AGGAGGCCTT CATGATCACA 
GTCAAGTTTG TGGAGGACAC CTGTCGGCTG GCCCTGGTGT ACTGCAGCCT GATAAAGGCC 
CGGGCCCGAG AGCTCTCTGC AGGCCAGAAG GACCAGGGCC AGGCAGCCAA TATGCTGTGC 
GTGGTGGTGA ACGACATGGA GCAGCTGCGG CTGGTGATCG GCAGGCTGCC CACCCAGCTG 
GCATGGGAGG CGCTGGAGCA GCGAGTAGGG GCTGTGCTGG AGCAGGGGCA GCTGCAGAAC 
ACGCTGCATG CCCAGCTGCA GGGTGCGCTG GCGGGGCTGG GCCACGAGAT CCGCACCGGC 
GTCCGCACCC TTGCGGAGCA GCTGGAGGTG GGCATCGCCA AGCACATCCA GAACCTCGTG 
GGCGTCAGGG AGTCCATCTT GCCTGAGGAT GCCATTCTGC CCCTGATGAA ATTCCTGGAG 
GTGGAGCTCT GCTACATGAA CACCAACTTG GTGCAGGAGA ACTTCAGCAG CCTTCTGACC 
CTGCTCTGGA CCCACACGCT CGAGGTGATG GTTGAGGGTG CCACCTCCCA GCGGAGCTGC 
CCCCTGGCCT CTAGCAGGCT GAAGATTGCG CTGCAGAACC TGGAGATCTG CTTCTACGCA 
GAGGGCTGTG GCCTGCAGCC CGAGGCCCTG CACACGGCCA CCTTCCAGGC TTTGCAGAGG 
GACTTAGAGC TGCAGGCGGC CTCCAGCCGG GAGCTCATCC AGAAGTACTT CTGCAACCGC 
ATCCAGCAGC AGGCGGAAAC CACCTCTGAG GAGCTCGGGG CGATCACCGT CAAGGCCTCC 
TACCGCGCCT CTGAGCAGAA GCTGCATGTG GAGGTGCTCA GCGCCTCCAG CCTGCTGCCC 
CTGGACTCCA ATGGCTCCAG CGACCCCTTT GTTCAGCTGA CCTTGGAGCC CAGGCACGAG 
TTTCCTGAGC TGGCCCCCCG GGAAACCCAA AAGCACAAGA AGGACCTTCA CCCGCTGTTT 
GATGAGACCT TTGAATTCCT GGTGCCTGCT GAGCCGTGCC AGAAGGACGG GGCGTGCCTC 
CTGCTCACAG TGCTGGACCA TGACACATTG GGGGCCGACG ACCTGGAGGG CGAGGCCTTT 
CTGCCGCTGC GCTCGGTGCT GGGCCTCAGT GGGACGGAGG AGCCTGGGGA GGTGCCTCAG 
AGCCGCCTGC CCCTCACCTA CCCCGCACCC AATGGGGACC CAATCCTGCA GCTTCTGGAG 
AGCCGGAAGG GTGATCGTGA GGCCCAGGTG TTTGTGAAGC TGCGGCGGCA GCGAGCCAAG 
CAGGCCTCCC AGCATGCCGT GCGGCTGGGG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006912275.1
CDS	130..3405
Translation	MATPLSHSQRRPPLLRQAIKIRRRRVRDLQGPLPHTAQEIEAPSHHFSPEERALLYEEALYTVLHRLGKPEPGHVSEASELLRYLQEAFHVEPEEHQRMLRQVQALEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGTLEGSPGSRRRQKAVVKHTIPEEQTHRTQVISQTLNPVWDETFILEFEDIRNSSFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPCTETYPDRGQCHLQFQLIHKRRATGASRSQPSYTVHLHLLQQLVSHEVTQHQAGSTSWDGLLSPQAATILFLHATQKDLSDFHQSMAQWLAYSRVYQSLEFPSSCLLHPITSIEYQWIQGRLKAEQMEELATSFSSLLAYGLSLIRRFRSVFPLSVSDSPARLQSLLRVLVQMCKMKAFRELCPDSGPLPHLVTEALRAGTVEWFHLKQQHHQPMVQGMLEAGKALLSLVQDIIGDLHQCQRTWNKIFHNALKIDLFAMAFLELQWLVAKRVQDHTAAALRGLVSPEMGESLFQLYIILKELCQLGPAPSERDGVLALDGFHRWFQPAIPSWLQKTYSVALARVQRAVQMDKLVPLGELTKHSTSAVDLSTCFAQISHTARQLDWPDPEEAFMITVKFVEDTCRLALVYCSLIKARARELSAGQKDQGQAANMLCVVVNDMEQLRLVIGRLPTQLAWEALEQRVGAVLEQGQLQNTLHAQLQGALAGLGHEIRTGVRTLAEQLEVGIAKHIQNLVGVRESILPEDAILPLMKFLEVELCYMNTNLVQENFSSLLTLLWTHTLEVMVEGATSQRSCPLASSRLKIALQNLEICFYAEGCGLQPEALHTATFQALQRDLELQAASSRELIQKYFCNRIQQQAETTSEELGAITVKASYRASEQKLHVEVLSASSLLPLDSNGSSDPFVQLTLEPRHEFPELAPRETQKHKKDLHPLFDETFEFLVPAEPCQKDGACLLLTVLDHDTLGADDLEGEAFLPLRSVLGLSGTEEPGEVPQSRLPLTYPAPNGDPILQLLESRKGDREAQVFVKLRRQRAKQASQHAVRLGQ

Target ORF information:

RefSeq Version	XM_006912213.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006912213.2

ORF Insert Sequence:

ATGGCGACAC CCCTCTCTCA CTCACAGCGG CGCCCGCCCC TCCTGCGCCA GGCCATCAAG 
ATAAGGCGCC GCAGGGTCAG AGACCTGCAG GGTCCCCTGC CCCACACTGC TCAGGAGATC 
GAGGCTCCAT CCCACCACTT CTCCCCTGAG GAGCGGGCCC TGCTCTACGA GGAAGCCCTC 
TACACTGTCC TGCACCGCCT GGGAAAGCCC GAGCCCGGCC ATGTGAGCGA GGCGTCCGAG 
CTGCTGCGCT ACCTGCAGGA GGCCTTCCAC GTGGAGCCCG AGGAACACCA GCGGATGCTG 
CGGCAGGTGC AGGCCCTCGA GAAGCCAATA TTTTGTCTGA AGGCAACGGT GAAACAAGCC 
AAGGGCATTC TGGGCAAGGA TGTCAGCGGG TTCAGTGACC CCTACTGCCT GCTGGGCATT 
GAGCAGGGAG TGGGCACGCT GGAGGGCAGC CCCGGGTCTC GGCGGCGGCA GAAGGCTGTG 
GTGAAGCACA CCATCCCGGA GGAGCAGACA CACCGCACCC AGGTCATCAG CCAGACACTC 
AACCCCGTCT GGGACGAGAC CTTCATCCTG GAGTTTGAGG ACATAAGAAA TTCGAGTTTT 
CATCTGGACA TGTGGGACCT GGACACCGTG GAGTCTGTCA GGCAGAAGCT TGGGGAGCTC 
ACAGATCTGC ACGGGCTGCG CAGGATCTTT AAGGAGGCCC GGAAGGACAA AGGCCAGGAC 
GACTTCCTGG GAAACGTGGT GCTGCGGCTG CAGGACCTTC GCTGCCGGGA GGATCAGTGG 
TACCCTCTGG AGCCCTGCAC TGAGACCTAC CCAGACCGTG GCCAGTGCCA CCTCCAGTTC 
CAGCTCATCC ACAAGCGGAG AGCCACTGGG GCCAGCCGCT CCCAGCCCAG CTACACGGTG 
CACCTCCACC TGCTGCAACA GCTCGTGTCC CACGAGGTCA CCCAGCACCA GGCAGGCAGC 
ACCTCCTGGG ATGGGCTGCT GAGTCCTCAG GCTGCCACCA TCCTGTTTCT CCATGCCACG 
CAGAAGGACC TGTCCGACTT CCACCAGTCC ATGGCGCAGT GGCTGGCCTA CAGCCGTGTC 
TACCAGAGCC TGGAGTTCCC CAGCAGCTGC CTCCTGCACC CTATCACCAG CATCGAGTAC 
CAGTGGATCC AGGGCCGGCT CAAGGCAGAG CAGATGGAGG AGCTGGCAAC CTCATTCAGC 
TCCCTGCTGG CATATGGCCT CTCCCTCATC CGGAGGTTCC GGTCTGTCTT CCCCTTATCT 
GTCTCGGACT CCCCAGCTCG GCTACAGTCT CTCCTCAGGG TCCTGGTACA GATGTGCAAG 
ATGAAGGCCT TTAGAGAACT GTGCCCTGAC AGTGGCCCAC TGCCCCACCT GGTGACTGAG 
GCGCTGCGGG CTGGCACCGT TGAATGGTTC CACCTGAAGC AGCAGCACCA TCAGCCAATG 
GTGCAGGGCA TGCTGGAGGC AGGCAAGGCC TTGCTGAGCC TGGTCCAAGA CATCATCGGT 
GACCTGCACC AGTGCCAGCG CACGTGGAAC AAGATCTTCC ACAATGCCCT TAAGATCGAC 
CTCTTCGCCA TGGCTTTCCT GGAGCTGCAG TGGCTGGTGG CCAAGCGCGT GCAGGACCAC 
ACGGCCGCGG CGTTGAGAGG CCTCGTGTCC CCAGAGATGG GAGAGAGTTT GTTCCAGCTC 
TACATCATCC TGAAGGAGCT CTGCCAGCTG GGCCCCGCCC CCTCGGAGAG GGATGGAGTC 
CTGGCCCTGG ATGGCTTCCA CCGCTGGTTC CAGCCTGCCA TCCCTTCCTG GCTGCAGAAG 
ACATACAGCG TGGCCTTGGC GCGGGTGCAG CGCGCTGTGC AGATGGACAA GCTGGTGCCC 
CTGGGTGAAC TGACCAAGCA TAGCACATCG GCCGTGGATC TGTCCACCTG CTTTGCTCAG 
ATCAGCCACA CTGCCCGGCA GCTGGACTGG CCCGACCCAG AGGAGGCCTT CATGATCACA 
GTCAAGTTTG TGGAGGACAC CTGTCGGCTG GCCCTGGTGT ACTGCAGCCT GATAAAGGCC 
CGGGCCCGAG AGCTCTCTGC AGGCCAGAAG GACCAGGGCC AGGCAGCCAA TATGCTGTGC 
GTGGTGGTGA ACGACATGGA GCAGCTGCGG CTGGTGATCG GCAGGCTGCC CACCCAGCTG 
GCATGGGAGG CGCTGGAGCA GCGAGTAGGG GCTGTGCTGG AGCAGGGGCA GCTGCAGAAC 
ACGCTGCATG CCCAGCTGCA GGGTGCGCTG GCGGGGCTGG GCCACGAGAT CCGCACCGGC 
GTCCGCACCC TTGCGGAGCA GCTGGAGGTG GGCATCGCCA AGCACATCCA GAACCTCGTG 
GGCGTCAGGG AGTCCATCTT GCCTGAGGAT GCCATTCTGC CCCTGATGAA ATTCCTGGAG 
GTGGAGCTCT GCTACATGAA CACCAACTTG GTGCAGGAGA ACTTCAGCAG CCTTCTGACC 
CTGCTCTGGA CCCACACGCT CGAGGTGATG GTTGAGGGTG CCACCTCCCA GCGGAGCTGC 
CCCCTGGCCT CTAGCAGGCT GAAGATTGCG CTGCAGAACC TGGAGATCTG CTTCTACGCA 
GAGGGCTGTG GCCTGCAGCC CGAGGCCCTG CACACGGCCA CCTTCCAGGC TTTGCAGAGG 
GACTTAGAGC TGCAGGCGGC CTCCAGCCGG GAGCTCATCC AGAAGTACTT CTGCAACCGC 
ATCCAGCAGC AGGCGGAAAC CACCTCTGAG GAGCTCGGGG CGATCACCGT CAAGGCCTCC 
TACCGCGCCT CTGAGCAGAA GCTGCATGTG GAGGTGCTCA GCGCCTCCAG CCTGCTGCCC 
CTGGACTCCA ATGGCTCCAG CGACCCCTTT GTTCAGCTGA CCTTGGAGCC CAGGCACGAG 
TTTCCTGAGC TGGCCCCCCG GGAAACCCAA AAGCACAAGA AGGACCTTCA CCCGCTGTTT 
GATGAGACCT TTGAATTCCT GGTGCCTGCT GAGCCGTGCC AGAAGGACGG GGCGTGCCTC 
CTGCTCACAG TGCTGGACCA TGACACATTG GGGGCCGACG ACCTGGAGGG CGAGGCCTTT 
CTGCCGCTGC GCTCGGTGCT GGGCCTCAGT GGGACGGAGG AGCCTGGGGA GGTGCCTCAG 
AGCCGCCTGC CCCTCACCTA CCCCGCACCC AATGGGGACC CAATCCTGCA GCTTCTGGAG 
AGCCGGAAGG GTGATCGTGA GGCCCAGGTG TTTGTGAAGC TGCGGCGGCA GCGAGCCAAG 
CAGGCCTCCC AGCATGCCGT GCGGCTGGGG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13D ( XM_015589777.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015445263.1 Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPt15191
Clone ID Related Accession (Same CDS sequence)	XM_006912213.2 , XM_015589777.1 , XM_015589777.2
Accession Version	XM_015589777.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	protein unc-13 homolog D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006436283.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACAC CCCTCTCTCA CTCACAGCGG CGCCCGCCCC TCCTGCGCCA GGCCATCAAG 
ATAAGGCGCC GCAGGGTCAG AGACCTGCAG GGTCCCCTGC CCCACACTGC TCAGGAGATC 
GAGGCTCCAT CCCACCACTT CTCCCCTGAG GAGCGGGCCC TGCTCTACGA GGAAGCCCTC 
TACACTGTCC TGCACCGCCT GGGAAAGCCC GAGCCCGGCC ATGTGAGCGA GGCGTCCGAG 
CTGCTGCGCT ACCTGCAGGA GGCCTTCCAC GTGGAGCCCG AGGAACACCA GCGGATGCTG 
CGGCAGGTGC AGGCCCTCGA GAAGCCAATA TTTTGTCTGA AGGCAACGGT GAAACAAGCC 
AAGGGCATTC TGGGCAAGGA TGTCAGCGGG TTCAGTGACC CCTACTGCCT GCTGGGCATT 
GAGCAGGGAG TGGGCACGCT GGAGGGCAGC CCCGGGTCTC GGCGGCGGCA GAAGGCTGTG 
GTGAAGCACA CCATCCCGGA GGAGCAGACA CACCGCACCC AGGTCATCAG CCAGACACTC 
AACCCCGTCT GGGACGAGAC CTTCATCCTG GAGTTTGAGG ACATAAGAAA TTCGAGTTTT 
CATCTGGACA TGTGGGACCT GGACACCGTG GAGTCTGTCA GGCAGAAGCT TGGGGAGCTC 
ACAGATCTGC ACGGGCTGCG CAGGATCTTT AAGGAGGCCC GGAAGGACAA AGGCCAGGAC 
GACTTCCTGG GAAACGTGGT GCTGCGGCTG CAGGACCTTC GCTGCCGGGA GGATCAGTGG 
TACCCTCTGG AGCCCTGCAC TGAGACCTAC CCAGACCGTG GCCAGTGCCA CCTCCAGTTC 
CAGCTCATCC ACAAGCGGAG AGCCACTGGG GCCAGCCGCT CCCAGCCCAG CTACACGGTG 
CACCTCCACC TGCTGCAACA GCTCGTGTCC CACGAGGTCA CCCAGCACCA GGCAGGCAGC 
ACCTCCTGGG ATGGGCTGCT GAGTCCTCAG GCTGCCACCA TCCTGTTTCT CCATGCCACG 
CAGAAGGACC TGTCCGACTT CCACCAGTCC ATGGCGCAGT GGCTGGCCTA CAGCCGTGTC 
TACCAGAGCC TGGAGTTCCC CAGCAGCTGC CTCCTGCACC CTATCACCAG CATCGAGTAC 
CAGTGGATCC AGGGCCGGCT CAAGGCAGAG CAGATGGAGG AGCTGGCAAC CTCATTCAGC 
TCCCTGCTGG CATATGGCCT CTCCCTCATC CGGAGGTTCC GGTCTGTCTT CCCCTTATCT 
GTCTCGGACT CCCCAGCTCG GCTACAGTCT CTCCTCAGGG TCCTGGTACA GATGTGCAAG 
ATGAAGGCCT TTAGAGAACT GTGCCCTGAC AGTGGCCCAC TGCCCCACCT GGTGACTGAG 
GCGCTGCGGG CTGGCACCGT TGAATGGTTC CACCTGAAGC AGCAGCACCA TCAGCCAATG 
GTGCAGGGCA TGCTGGAGGC AGGCAAGGCC TTGCTGAGCC TGGTCCAAGA CATCATCGGT 
GACCTGCACC AGTGCCAGCG CACGTGGAAC AAGATCTTCC ACAATGCCCT TAAGATCGAC 
CTCTTCGCCA TGGCTTTCCT GGAGCTGCAG TGGCTGGTGG CCAAGCGCGT GCAGGACCAC 
ACGGCCGCGG CGTTGAGAGG CCTCGTGTCC CCAGAGATGG GAGAGAGTTT GTTCCAGCTC 
TACATCATCC TGAAGGAGCT CTGCCAGCTG GGCCCCGCCC CCTCGGAGAG GGATGGAGTC 
CTGGCCCTGG ATGGCTTCCA CCGCTGGTTC CAGCCTGCCA TCCCTTCCTG GCTGCAGAAG 
ACATACAGCG TGGCCTTGGC GCGGGTGCAG CGCGCTGTGC AGATGGACAA GCTGGTGCCC 
CTGGGTGAAC TGACCAAGCA TAGCACATCG GCCGTGGATC TGTCCACCTG CTTTGCTCAG 
ATCAGCCACA CTGCCCGGCA GCTGGACTGG CCCGACCCAG AGGAGGCCTT CATGATCACA 
GTCAAGTTTG TGGAGGACAC CTGTCGGCTG GCCCTGGTGT ACTGCAGCCT GATAAAGGCC 
CGGGCCCGAG AGCTCTCTGC AGGCCAGAAG GACCAGGGCC AGGCAGCCAA TATGCTGTGC 
GTGGTGGTGA ACGACATGGA GCAGCTGCGG CTGGTGATCG GCAGGCTGCC CACCCAGCTG 
GCATGGGAGG CGCTGGAGCA GCGAGTAGGG GCTGTGCTGG AGCAGGGGCA GCTGCAGAAC 
ACGCTGCATG CCCAGCTGCA GGGTGCGCTG GCGGGGCTGG GCCACGAGAT CCGCACCGGC 
GTCCGCACCC TTGCGGAGCA GCTGGAGGTG GGCATCGCCA AGCACATCCA GAACCTCGTG 
GGCGTCAGGG AGTCCATCTT GCCTGAGGAT GCCATTCTGC CCCTGATGAA ATTCCTGGAG 
GTGGAGCTCT GCTACATGAA CACCAACTTG GTGCAGGAGA ACTTCAGCAG CCTTCTGACC 
CTGCTCTGGA CCCACACGCT CGAGGTGATG GTTGAGGGTG CCACCTCCCA GCGGAGCTGC 
CCCCTGGCCT CTAGCAGGCT GAAGATTGCG CTGCAGAACC TGGAGATCTG CTTCTACGCA 
GAGGGCTGTG GCCTGCAGCC CGAGGCCCTG CACACGGCCA CCTTCCAGGC TTTGCAGAGG 
GACTTAGAGC TGCAGGCGGC CTCCAGCCGG GAGCTCATCC AGAAGTACTT CTGCAACCGC 
ATCCAGCAGC AGGCGGAAAC CACCTCTGAG GAGCTCGGGG CGATCACCGT CAAGGCCTCC 
TACCGCGCCT CTGAGCAGAA GCTGCATGTG GAGGTGCTCA GCGCCTCCAG CCTGCTGCCC 
CTGGACTCCA ATGGCTCCAG CGACCCCTTT GTTCAGCTGA CCTTGGAGCC CAGGCACGAG 
TTTCCTGAGC TGGCCCCCCG GGAAACCCAA AAGCACAAGA AGGACCTTCA CCCGCTGTTT 
GATGAGACCT TTGAATTCCT GGTGCCTGCT GAGCCGTGCC AGAAGGACGG GGCGTGCCTC 
CTGCTCACAG TGCTGGACCA TGACACATTG GGGGCCGACG ACCTGGAGGG CGAGGCCTTT 
CTGCCGCTGC GCTCGGTGCT GGGCCTCAGT GGGACGGAGG AGCCTGGGGA GGTGCCTCAG 
AGCCGCCTGC CCCTCACCTA CCCCGCACCC AATGGGGACC CAATCCTGCA GCTTCTGGAG 
AGCCGGAAGG GTGATCGTGA GGCCCAGGTG TTTGTGAAGC TGCGGCGGCA GCGAGCCAAG 
CAGGCCTCCC AGCATGCCGT GCGGCTGGGG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015445263.1
CDS	83..3358
Translation	MATPLSHSQRRPPLLRQAIKIRRRRVRDLQGPLPHTAQEIEAPSHHFSPEERALLYEEALYTVLHRLGKPEPGHVSEASELLRYLQEAFHVEPEEHQRMLRQVQALEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGTLEGSPGSRRRQKAVVKHTIPEEQTHRTQVISQTLNPVWDETFILEFEDIRNSSFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPCTETYPDRGQCHLQFQLIHKRRATGASRSQPSYTVHLHLLQQLVSHEVTQHQAGSTSWDGLLSPQAATILFLHATQKDLSDFHQSMAQWLAYSRVYQSLEFPSSCLLHPITSIEYQWIQGRLKAEQMEELATSFSSLLAYGLSLIRRFRSVFPLSVSDSPARLQSLLRVLVQMCKMKAFRELCPDSGPLPHLVTEALRAGTVEWFHLKQQHHQPMVQGMLEAGKALLSLVQDIIGDLHQCQRTWNKIFHNALKIDLFAMAFLELQWLVAKRVQDHTAAALRGLVSPEMGESLFQLYIILKELCQLGPAPSERDGVLALDGFHRWFQPAIPSWLQKTYSVALARVQRAVQMDKLVPLGELTKHSTSAVDLSTCFAQISHTARQLDWPDPEEAFMITVKFVEDTCRLALVYCSLIKARARELSAGQKDQGQAANMLCVVVNDMEQLRLVIGRLPTQLAWEALEQRVGAVLEQGQLQNTLHAQLQGALAGLGHEIRTGVRTLAEQLEVGIAKHIQNLVGVRESILPEDAILPLMKFLEVELCYMNTNLVQENFSSLLTLLWTHTLEVMVEGATSQRSCPLASSRLKIALQNLEICFYAEGCGLQPEALHTATFQALQRDLELQAASSRELIQKYFCNRIQQQAETTSEELGAITVKASYRASEQKLHVEVLSASSLLPLDSNGSSDPFVQLTLEPRHEFPELAPRETQKHKKDLHPLFDETFEFLVPAEPCQKDGACLLLTVLDHDTLGADDLEGEAFLPLRSVLGLSGTEEPGEVPQSRLPLTYPAPNGDPILQLLESRKGDREAQVFVKLRRQRAKQASQHAVRLGQ

Target ORF information:

RefSeq Version	XM_015589777.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015589777.1

ORF Insert Sequence:

ATGGCGACAC CCCTCTCTCA CTCACAGCGG CGCCCGCCCC TCCTGCGCCA GGCCATCAAG 
ATAAGGCGCC GCAGGGTCAG AGACCTGCAG GGTCCCCTGC CCCACACTGC TCAGGAGATC 
GAGGCTCCAT CCCACCACTT CTCCCCTGAG GAGCGGGCCC TGCTCTACGA GGAAGCCCTC 
TACACTGTCC TGCACCGCCT GGGAAAGCCC GAGCCCGGCC ATGTGAGCGA GGCGTCCGAG 
CTGCTGCGCT ACCTGCAGGA GGCCTTCCAC GTGGAGCCCG AGGAACACCA GCGGATGCTG 
CGGCAGGTGC AGGCCCTCGA GAAGCCAATA TTTTGTCTGA AGGCAACGGT GAAACAAGCC 
AAGGGCATTC TGGGCAAGGA TGTCAGCGGG TTCAGTGACC CCTACTGCCT GCTGGGCATT 
GAGCAGGGAG TGGGCACGCT GGAGGGCAGC CCCGGGTCTC GGCGGCGGCA GAAGGCTGTG 
GTGAAGCACA CCATCCCGGA GGAGCAGACA CACCGCACCC AGGTCATCAG CCAGACACTC 
AACCCCGTCT GGGACGAGAC CTTCATCCTG GAGTTTGAGG ACATAAGAAA TTCGAGTTTT 
CATCTGGACA TGTGGGACCT GGACACCGTG GAGTCTGTCA GGCAGAAGCT TGGGGAGCTC 
ACAGATCTGC ACGGGCTGCG CAGGATCTTT AAGGAGGCCC GGAAGGACAA AGGCCAGGAC 
GACTTCCTGG GAAACGTGGT GCTGCGGCTG CAGGACCTTC GCTGCCGGGA GGATCAGTGG 
TACCCTCTGG AGCCCTGCAC TGAGACCTAC CCAGACCGTG GCCAGTGCCA CCTCCAGTTC 
CAGCTCATCC ACAAGCGGAG AGCCACTGGG GCCAGCCGCT CCCAGCCCAG CTACACGGTG 
CACCTCCACC TGCTGCAACA GCTCGTGTCC CACGAGGTCA CCCAGCACCA GGCAGGCAGC 
ACCTCCTGGG ATGGGCTGCT GAGTCCTCAG GCTGCCACCA TCCTGTTTCT CCATGCCACG 
CAGAAGGACC TGTCCGACTT CCACCAGTCC ATGGCGCAGT GGCTGGCCTA CAGCCGTGTC 
TACCAGAGCC TGGAGTTCCC CAGCAGCTGC CTCCTGCACC CTATCACCAG CATCGAGTAC 
CAGTGGATCC AGGGCCGGCT CAAGGCAGAG CAGATGGAGG AGCTGGCAAC CTCATTCAGC 
TCCCTGCTGG CATATGGCCT CTCCCTCATC CGGAGGTTCC GGTCTGTCTT CCCCTTATCT 
GTCTCGGACT CCCCAGCTCG GCTACAGTCT CTCCTCAGGG TCCTGGTACA GATGTGCAAG 
ATGAAGGCCT TTAGAGAACT GTGCCCTGAC AGTGGCCCAC TGCCCCACCT GGTGACTGAG 
GCGCTGCGGG CTGGCACCGT TGAATGGTTC CACCTGAAGC AGCAGCACCA TCAGCCAATG 
GTGCAGGGCA TGCTGGAGGC AGGCAAGGCC TTGCTGAGCC TGGTCCAAGA CATCATCGGT 
GACCTGCACC AGTGCCAGCG CACGTGGAAC AAGATCTTCC ACAATGCCCT TAAGATCGAC 
CTCTTCGCCA TGGCTTTCCT GGAGCTGCAG TGGCTGGTGG CCAAGCGCGT GCAGGACCAC 
ACGGCCGCGG CGTTGAGAGG CCTCGTGTCC CCAGAGATGG GAGAGAGTTT GTTCCAGCTC 
TACATCATCC TGAAGGAGCT CTGCCAGCTG GGCCCCGCCC CCTCGGAGAG GGATGGAGTC 
CTGGCCCTGG ATGGCTTCCA CCGCTGGTTC CAGCCTGCCA TCCCTTCCTG GCTGCAGAAG 
ACATACAGCG TGGCCTTGGC GCGGGTGCAG CGCGCTGTGC AGATGGACAA GCTGGTGCCC 
CTGGGTGAAC TGACCAAGCA TAGCACATCG GCCGTGGATC TGTCCACCTG CTTTGCTCAG 
ATCAGCCACA CTGCCCGGCA GCTGGACTGG CCCGACCCAG AGGAGGCCTT CATGATCACA 
GTCAAGTTTG TGGAGGACAC CTGTCGGCTG GCCCTGGTGT ACTGCAGCCT GATAAAGGCC 
CGGGCCCGAG AGCTCTCTGC AGGCCAGAAG GACCAGGGCC AGGCAGCCAA TATGCTGTGC 
GTGGTGGTGA ACGACATGGA GCAGCTGCGG CTGGTGATCG GCAGGCTGCC CACCCAGCTG 
GCATGGGAGG CGCTGGAGCA GCGAGTAGGG GCTGTGCTGG AGCAGGGGCA GCTGCAGAAC 
ACGCTGCATG CCCAGCTGCA GGGTGCGCTG GCGGGGCTGG GCCACGAGAT CCGCACCGGC 
GTCCGCACCC TTGCGGAGCA GCTGGAGGTG GGCATCGCCA AGCACATCCA GAACCTCGTG 
GGCGTCAGGG AGTCCATCTT GCCTGAGGAT GCCATTCTGC CCCTGATGAA ATTCCTGGAG 
GTGGAGCTCT GCTACATGAA CACCAACTTG GTGCAGGAGA ACTTCAGCAG CCTTCTGACC 
CTGCTCTGGA CCCACACGCT CGAGGTGATG GTTGAGGGTG CCACCTCCCA GCGGAGCTGC 
CCCCTGGCCT CTAGCAGGCT GAAGATTGCG CTGCAGAACC TGGAGATCTG CTTCTACGCA 
GAGGGCTGTG GCCTGCAGCC CGAGGCCCTG CACACGGCCA CCTTCCAGGC TTTGCAGAGG 
GACTTAGAGC TGCAGGCGGC CTCCAGCCGG GAGCTCATCC AGAAGTACTT CTGCAACCGC 
ATCCAGCAGC AGGCGGAAAC CACCTCTGAG GAGCTCGGGG CGATCACCGT CAAGGCCTCC 
TACCGCGCCT CTGAGCAGAA GCTGCATGTG GAGGTGCTCA GCGCCTCCAG CCTGCTGCCC 
CTGGACTCCA ATGGCTCCAG CGACCCCTTT GTTCAGCTGA CCTTGGAGCC CAGGCACGAG 
TTTCCTGAGC TGGCCCCCCG GGAAACCCAA AAGCACAAGA AGGACCTTCA CCCGCTGTTT 
GATGAGACCT TTGAATTCCT GGTGCCTGCT GAGCCGTGCC AGAAGGACGG GGCGTGCCTC 
CTGCTCACAG TGCTGGACCA TGACACATTG GGGGCCGACG ACCTGGAGGG CGAGGCCTTT 
CTGCCGCTGC GCTCGGTGCT GGGCCTCAGT GGGACGGAGG AGCCTGGGGA GGTGCCTCAG 
AGCCGCCTGC CCCTCACCTA CCCCGCACCC AATGGGGACC CAATCCTGCA GCTTCTGGAG 
AGCCGGAAGG GTGATCGTGA GGCCCAGGTG TTTGTGAAGC TGCGGCGGCA GCGAGCCAAG 
CAGGCCTCCC AGCATGCCGT GCGGCTGGGG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13D ( XM_015589777.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015445263.1 Pteropus alecto unc-13 homolog D (UNC13D), transcript variant X2, mRNA.

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CloneID	OPt15191
Clone ID Related Accession (Same CDS sequence)	XM_006912213.2 , XM_015589777.1 , XM_015589777.2
Accession Version	XM_015589777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	protein unc-13 homolog D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006436283.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015589777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACAC CCCTCTCTCA CTCACAGCGG CGCCCGCCCC TCCTGCGCCA GGCCATCAAG 
ATAAGGCGCC GCAGGGTCAG AGACCTGCAG GGTCCCCTGC CCCACACTGC TCAGGAGATC 
GAGGCTCCAT CCCACCACTT CTCCCCTGAG GAGCGGGCCC TGCTCTACGA GGAAGCCCTC 
TACACTGTCC TGCACCGCCT GGGAAAGCCC GAGCCCGGCC ATGTGAGCGA GGCGTCCGAG 
CTGCTGCGCT ACCTGCAGGA GGCCTTCCAC GTGGAGCCCG AGGAACACCA GCGGATGCTG 
CGGCAGGTGC AGGCCCTCGA GAAGCCAATA TTTTGTCTGA AGGCAACGGT GAAACAAGCC 
AAGGGCATTC TGGGCAAGGA TGTCAGCGGG TTCAGTGACC CCTACTGCCT GCTGGGCATT 
GAGCAGGGAG TGGGCACGCT GGAGGGCAGC CCCGGGTCTC GGCGGCGGCA GAAGGCTGTG 
GTGAAGCACA CCATCCCGGA GGAGCAGACA CACCGCACCC AGGTCATCAG CCAGACACTC 
AACCCCGTCT GGGACGAGAC CTTCATCCTG GAGTTTGAGG ACATAAGAAA TTCGAGTTTT 
CATCTGGACA TGTGGGACCT GGACACCGTG GAGTCTGTCA GGCAGAAGCT TGGGGAGCTC 
ACAGATCTGC ACGGGCTGCG CAGGATCTTT AAGGAGGCCC GGAAGGACAA AGGCCAGGAC 
GACTTCCTGG GAAACGTGGT GCTGCGGCTG CAGGACCTTC GCTGCCGGGA GGATCAGTGG 
TACCCTCTGG AGCCCTGCAC TGAGACCTAC CCAGACCGTG GCCAGTGCCA CCTCCAGTTC 
CAGCTCATCC ACAAGCGGAG AGCCACTGGG GCCAGCCGCT CCCAGCCCAG CTACACGGTG 
CACCTCCACC TGCTGCAACA GCTCGTGTCC CACGAGGTCA CCCAGCACCA GGCAGGCAGC 
ACCTCCTGGG ATGGGCTGCT GAGTCCTCAG GCTGCCACCA TCCTGTTTCT CCATGCCACG 
CAGAAGGACC TGTCCGACTT CCACCAGTCC ATGGCGCAGT GGCTGGCCTA CAGCCGTGTC 
TACCAGAGCC TGGAGTTCCC CAGCAGCTGC CTCCTGCACC CTATCACCAG CATCGAGTAC 
CAGTGGATCC AGGGCCGGCT CAAGGCAGAG CAGATGGAGG AGCTGGCAAC CTCATTCAGC 
TCCCTGCTGG CATATGGCCT CTCCCTCATC CGGAGGTTCC GGTCTGTCTT CCCCTTATCT 
GTCTCGGACT CCCCAGCTCG GCTACAGTCT CTCCTCAGGG TCCTGGTACA GATGTGCAAG 
ATGAAGGCCT TTAGAGAACT GTGCCCTGAC AGTGGCCCAC TGCCCCACCT GGTGACTGAG 
GCGCTGCGGG CTGGCACCGT TGAATGGTTC CACCTGAAGC AGCAGCACCA TCAGCCAATG 
GTGCAGGGCA TGCTGGAGGC AGGCAAGGCC TTGCTGAGCC TGGTCCAAGA CATCATCGGT 
GACCTGCACC AGTGCCAGCG CACGTGGAAC AAGATCTTCC ACAATGCCCT TAAGATCGAC 
CTCTTCGCCA TGGCTTTCCT GGAGCTGCAG TGGCTGGTGG CCAAGCGCGT GCAGGACCAC 
ACGGCCGCGG CGTTGAGAGG CCTCGTGTCC CCAGAGATGG GAGAGAGTTT GTTCCAGCTC 
TACATCATCC TGAAGGAGCT CTGCCAGCTG GGCCCCGCCC CCTCGGAGAG GGATGGAGTC 
CTGGCCCTGG ATGGCTTCCA CCGCTGGTTC CAGCCTGCCA TCCCTTCCTG GCTGCAGAAG 
ACATACAGCG TGGCCTTGGC GCGGGTGCAG CGCGCTGTGC AGATGGACAA GCTGGTGCCC 
CTGGGTGAAC TGACCAAGCA TAGCACATCG GCCGTGGATC TGTCCACCTG CTTTGCTCAG 
ATCAGCCACA CTGCCCGGCA GCTGGACTGG CCCGACCCAG AGGAGGCCTT CATGATCACA 
GTCAAGTTTG TGGAGGACAC CTGTCGGCTG GCCCTGGTGT ACTGCAGCCT GATAAAGGCC 
CGGGCCCGAG AGCTCTCTGC AGGCCAGAAG GACCAGGGCC AGGCAGCCAA TATGCTGTGC 
GTGGTGGTGA ACGACATGGA GCAGCTGCGG CTGGTGATCG GCAGGCTGCC CACCCAGCTG 
GCATGGGAGG CGCTGGAGCA GCGAGTAGGG GCTGTGCTGG AGCAGGGGCA GCTGCAGAAC 
ACGCTGCATG CCCAGCTGCA GGGTGCGCTG GCGGGGCTGG GCCACGAGAT CCGCACCGGC 
GTCCGCACCC TTGCGGAGCA GCTGGAGGTG GGCATCGCCA AGCACATCCA GAACCTCGTG 
GGCGTCAGGG AGTCCATCTT GCCTGAGGAT GCCATTCTGC CCCTGATGAA ATTCCTGGAG 
GTGGAGCTCT GCTACATGAA CACCAACTTG GTGCAGGAGA ACTTCAGCAG CCTTCTGACC 
CTGCTCTGGA CCCACACGCT CGAGGTGATG GTTGAGGGTG CCACCTCCCA GCGGAGCTGC 
CCCCTGGCCT CTAGCAGGCT GAAGATTGCG CTGCAGAACC TGGAGATCTG CTTCTACGCA 
GAGGGCTGTG GCCTGCAGCC CGAGGCCCTG CACACGGCCA CCTTCCAGGC TTTGCAGAGG 
GACTTAGAGC TGCAGGCGGC CTCCAGCCGG GAGCTCATCC AGAAGTACTT CTGCAACCGC 
ATCCAGCAGC AGGCGGAAAC CACCTCTGAG GAGCTCGGGG CGATCACCGT CAAGGCCTCC 
TACCGCGCCT CTGAGCAGAA GCTGCATGTG GAGGTGCTCA GCGCCTCCAG CCTGCTGCCC 
CTGGACTCCA ATGGCTCCAG CGACCCCTTT GTTCAGCTGA CCTTGGAGCC CAGGCACGAG 
TTTCCTGAGC TGGCCCCCCG GGAAACCCAA AAGCACAAGA AGGACCTTCA CCCGCTGTTT 
GATGAGACCT TTGAATTCCT GGTGCCTGCT GAGCCGTGCC AGAAGGACGG GGCGTGCCTC 
CTGCTCACAG TGCTGGACCA TGACACATTG GGGGCCGACG ACCTGGAGGG CGAGGCCTTT 
CTGCCGCTGC GCTCGGTGCT GGGCCTCAGT GGGACGGAGG AGCCTGGGGA GGTGCCTCAG 
AGCCGCCTGC CCCTCACCTA CCCCGCACCC AATGGGGACC CAATCCTGCA GCTTCTGGAG 
AGCCGGAAGG GTGATCGTGA GGCCCAGGTG TTTGTGAAGC TGCGGCGGCA GCGAGCCAAG 
CAGGCCTCCC AGCATGCCGT GCGGCTGGGG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015445263.1
CDS	168..3443
Translation	MATPLSHSQRRPPLLRQAIKIRRRRVRDLQGPLPHTAQEIEAPSHHFSPEERALLYEEALYTVLHRLGKPEPGHVSEASELLRYLQEAFHVEPEEHQRMLRQVQALEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGTLEGSPGSRRRQKAVVKHTIPEEQTHRTQVISQTLNPVWDETFILEFEDIRNSSFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPCTETYPDRGQCHLQFQLIHKRRATGASRSQPSYTVHLHLLQQLVSHEVTQHQAGSTSWDGLLSPQAATILFLHATQKDLSDFHQSMAQWLAYSRVYQSLEFPSSCLLHPITSIEYQWIQGRLKAEQMEELATSFSSLLAYGLSLIRRFRSVFPLSVSDSPARLQSLLRVLVQMCKMKAFRELCPDSGPLPHLVTEALRAGTVEWFHLKQQHHQPMVQGMLEAGKALLSLVQDIIGDLHQCQRTWNKIFHNALKIDLFAMAFLELQWLVAKRVQDHTAAALRGLVSPEMGESLFQLYIILKELCQLGPAPSERDGVLALDGFHRWFQPAIPSWLQKTYSVALARVQRAVQMDKLVPLGELTKHSTSAVDLSTCFAQISHTARQLDWPDPEEAFMITVKFVEDTCRLALVYCSLIKARARELSAGQKDQGQAANMLCVVVNDMEQLRLVIGRLPTQLAWEALEQRVGAVLEQGQLQNTLHAQLQGALAGLGHEIRTGVRTLAEQLEVGIAKHIQNLVGVRESILPEDAILPLMKFLEVELCYMNTNLVQENFSSLLTLLWTHTLEVMVEGATSQRSCPLASSRLKIALQNLEICFYAEGCGLQPEALHTATFQALQRDLELQAASSRELIQKYFCNRIQQQAETTSEELGAITVKASYRASEQKLHVEVLSASSLLPLDSNGSSDPFVQLTLEPRHEFPELAPRETQKHKKDLHPLFDETFEFLVPAEPCQKDGACLLLTVLDHDTLGADDLEGEAFLPLRSVLGLSGTEEPGEVPQSRLPLTYPAPNGDPILQLLESRKGDREAQVFVKLRRQRAKQASQHAVRLGQ

Target ORF information:

RefSeq Version	XM_015589777.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto unc-13 homolog D (UNC13D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015589777.2

ORF Insert Sequence:

ATGGCGACAC CCCTCTCTCA CTCACAGCGG CGCCCGCCCC TCCTGCGCCA GGCCATCAAG 
ATAAGGCGCC GCAGGGTCAG AGACCTGCAG GGTCCCCTGC CCCACACTGC TCAGGAGATC 
GAGGCTCCAT CCCACCACTT CTCCCCTGAG GAGCGGGCCC TGCTCTACGA GGAAGCCCTC 
TACACTGTCC TGCACCGCCT GGGAAAGCCC GAGCCCGGCC ATGTGAGCGA GGCGTCCGAG 
CTGCTGCGCT ACCTGCAGGA GGCCTTCCAC GTGGAGCCCG AGGAACACCA GCGGATGCTG 
CGGCAGGTGC AGGCCCTCGA GAAGCCAATA TTTTGTCTGA AGGCAACGGT GAAACAAGCC 
AAGGGCATTC TGGGCAAGGA TGTCAGCGGG TTCAGTGACC CCTACTGCCT GCTGGGCATT 
GAGCAGGGAG TGGGCACGCT GGAGGGCAGC CCCGGGTCTC GGCGGCGGCA GAAGGCTGTG 
GTGAAGCACA CCATCCCGGA GGAGCAGACA CACCGCACCC AGGTCATCAG CCAGACACTC 
AACCCCGTCT GGGACGAGAC CTTCATCCTG GAGTTTGAGG ACATAAGAAA TTCGAGTTTT 
CATCTGGACA TGTGGGACCT GGACACCGTG GAGTCTGTCA GGCAGAAGCT TGGGGAGCTC 
ACAGATCTGC ACGGGCTGCG CAGGATCTTT AAGGAGGCCC GGAAGGACAA AGGCCAGGAC 
GACTTCCTGG GAAACGTGGT GCTGCGGCTG CAGGACCTTC GCTGCCGGGA GGATCAGTGG 
TACCCTCTGG AGCCCTGCAC TGAGACCTAC CCAGACCGTG GCCAGTGCCA CCTCCAGTTC 
CAGCTCATCC ACAAGCGGAG AGCCACTGGG GCCAGCCGCT CCCAGCCCAG CTACACGGTG 
CACCTCCACC TGCTGCAACA GCTCGTGTCC CACGAGGTCA CCCAGCACCA GGCAGGCAGC 
ACCTCCTGGG ATGGGCTGCT GAGTCCTCAG GCTGCCACCA TCCTGTTTCT CCATGCCACG 
CAGAAGGACC TGTCCGACTT CCACCAGTCC ATGGCGCAGT GGCTGGCCTA CAGCCGTGTC 
TACCAGAGCC TGGAGTTCCC CAGCAGCTGC CTCCTGCACC CTATCACCAG CATCGAGTAC 
CAGTGGATCC AGGGCCGGCT CAAGGCAGAG CAGATGGAGG AGCTGGCAAC CTCATTCAGC 
TCCCTGCTGG CATATGGCCT CTCCCTCATC CGGAGGTTCC GGTCTGTCTT CCCCTTATCT 
GTCTCGGACT CCCCAGCTCG GCTACAGTCT CTCCTCAGGG TCCTGGTACA GATGTGCAAG 
ATGAAGGCCT TTAGAGAACT GTGCCCTGAC AGTGGCCCAC TGCCCCACCT GGTGACTGAG 
GCGCTGCGGG CTGGCACCGT TGAATGGTTC CACCTGAAGC AGCAGCACCA TCAGCCAATG 
GTGCAGGGCA TGCTGGAGGC AGGCAAGGCC TTGCTGAGCC TGGTCCAAGA CATCATCGGT 
GACCTGCACC AGTGCCAGCG CACGTGGAAC AAGATCTTCC ACAATGCCCT TAAGATCGAC 
CTCTTCGCCA TGGCTTTCCT GGAGCTGCAG TGGCTGGTGG CCAAGCGCGT GCAGGACCAC 
ACGGCCGCGG CGTTGAGAGG CCTCGTGTCC CCAGAGATGG GAGAGAGTTT GTTCCAGCTC 
TACATCATCC TGAAGGAGCT CTGCCAGCTG GGCCCCGCCC CCTCGGAGAG GGATGGAGTC 
CTGGCCCTGG ATGGCTTCCA CCGCTGGTTC CAGCCTGCCA TCCCTTCCTG GCTGCAGAAG 
ACATACAGCG TGGCCTTGGC GCGGGTGCAG CGCGCTGTGC AGATGGACAA GCTGGTGCCC 
CTGGGTGAAC TGACCAAGCA TAGCACATCG GCCGTGGATC TGTCCACCTG CTTTGCTCAG 
ATCAGCCACA CTGCCCGGCA GCTGGACTGG CCCGACCCAG AGGAGGCCTT CATGATCACA 
GTCAAGTTTG TGGAGGACAC CTGTCGGCTG GCCCTGGTGT ACTGCAGCCT GATAAAGGCC 
CGGGCCCGAG AGCTCTCTGC AGGCCAGAAG GACCAGGGCC AGGCAGCCAA TATGCTGTGC 
GTGGTGGTGA ACGACATGGA GCAGCTGCGG CTGGTGATCG GCAGGCTGCC CACCCAGCTG 
GCATGGGAGG CGCTGGAGCA GCGAGTAGGG GCTGTGCTGG AGCAGGGGCA GCTGCAGAAC 
ACGCTGCATG CCCAGCTGCA GGGTGCGCTG GCGGGGCTGG GCCACGAGAT CCGCACCGGC 
GTCCGCACCC TTGCGGAGCA GCTGGAGGTG GGCATCGCCA AGCACATCCA GAACCTCGTG 
GGCGTCAGGG AGTCCATCTT GCCTGAGGAT GCCATTCTGC CCCTGATGAA ATTCCTGGAG 
GTGGAGCTCT GCTACATGAA CACCAACTTG GTGCAGGAGA ACTTCAGCAG CCTTCTGACC 
CTGCTCTGGA CCCACACGCT CGAGGTGATG GTTGAGGGTG CCACCTCCCA GCGGAGCTGC 
CCCCTGGCCT CTAGCAGGCT GAAGATTGCG CTGCAGAACC TGGAGATCTG CTTCTACGCA 
GAGGGCTGTG GCCTGCAGCC CGAGGCCCTG CACACGGCCA CCTTCCAGGC TTTGCAGAGG 
GACTTAGAGC TGCAGGCGGC CTCCAGCCGG GAGCTCATCC AGAAGTACTT CTGCAACCGC 
ATCCAGCAGC AGGCGGAAAC CACCTCTGAG GAGCTCGGGG CGATCACCGT CAAGGCCTCC 
TACCGCGCCT CTGAGCAGAA GCTGCATGTG GAGGTGCTCA GCGCCTCCAG CCTGCTGCCC 
CTGGACTCCA ATGGCTCCAG CGACCCCTTT GTTCAGCTGA CCTTGGAGCC CAGGCACGAG 
TTTCCTGAGC TGGCCCCCCG GGAAACCCAA AAGCACAAGA AGGACCTTCA CCCGCTGTTT 
GATGAGACCT TTGAATTCCT GGTGCCTGCT GAGCCGTGCC AGAAGGACGG GGCGTGCCTC 
CTGCTCACAG TGCTGGACCA TGACACATTG GGGGCCGACG ACCTGGAGGG CGAGGCCTTT 
CTGCCGCTGC GCTCGGTGCT GGGCCTCAGT GGGACGGAGG AGCCTGGGGA GGTGCCTCAG 
AGCCGCCTGC CCCTCACCTA CCCCGCACCC AATGGGGACC CAATCCTGCA GCTTCTGGAG 
AGCCGGAAGG GTGATCGTGA GGCCCAGGTG TTTGTGAAGC TGCGGCGGCA GCGAGCCAAG 
CAGGCCTCCC AGCATGCCGT GCGGCTGGGG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13D ( XM_015589778.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015445264.1 Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPt15192
Clone ID Related Accession (Same CDS sequence)	XM_015589778.1 , XM_015589778.2
Accession Version	XM_015589778.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	protein unc-13 homolog D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006436283.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTCA GCCACAGCAG GCCAGAGGAG GACGAGGGCG ACCTGGGGGC CAGACCCTCA 
GGAGGTCTGC TGGGGGAACA GATCGAGGCT CCATCCCACC ACTTCTCCCC TGAGGAGCGG 
GCCCTGCTCT ACGAGGAAGC CCTCTACACT GTCCTGCACC GCCTGGGAAA GCCCGAGCCC 
GGCCATGTGA GCGAGGCGTC CGAGCTGCTG CGCTACCTGC AGGAGGCCTT CCACGTGGAG 
CCCGAGGAAC ACCAGCGGAT GCTGCGGCAG GTGCAGGCCC TCGAGAAGCC AATATTTTGT 
CTGAAGGCAA CGGTGAAACA AGCCAAGGGC ATTCTGGGCA AGGATGTCAG CGGGTTCAGT 
GACCCCTACT GCCTGCTGGG CATTGAGCAG GGAGTGGGCA CGCTGGAGGG CAGCCCCGGG 
TCTCGGCGGC GGCAGAAGGC TGTGGTGAAG CACACCATCC CGGAGGAGCA GACACACCGC 
ACCCAGGTCA TCAGCCAGAC ACTCAACCCC GTCTGGGACG AGACCTTCAT CCTGGAGTTT 
GAGGACATAA GAAATTCGAG TTTTCATCTG GACATGTGGG ACCTGGACAC CGTGGAGTCT 
GTCAGGCAGA AGCTTGGGGA GCTCACAGAT CTGCACGGGC TGCGCAGGAT CTTTAAGGAG 
GCCCGGAAGG ACAAAGGCCA GGACGACTTC CTGGGAAACG TGGTGCTGCG GCTGCAGGAC 
CTTCGCTGCC GGGAGGATCA GTGGTACCCT CTGGAGCCCT GCACTGAGAC CTACCCAGAC 
CGTGGCCAGT GCCACCTCCA GTTCCAGCTC ATCCACAAGC GGAGAGCCAC TGGGGCCAGC 
CGCTCCCAGC CCAGCTACAC GGTGCACCTC CACCTGCTGC AACAGCTCGT GTCCCACGAG 
GTCACCCAGC ACCAGGCAGG CAGCACCTCC TGGGATGGGC TGCTGAGTCC TCAGGCTGCC 
ACCATCCTGT TTCTCCATGC CACGCAGAAG GACCTGTCCG ACTTCCACCA GTCCATGGCG 
CAGTGGCTGG CCTACAGCCG TGTCTACCAG AGCCTGGAGT TCCCCAGCAG CTGCCTCCTG 
CACCCTATCA CCAGCATCGA GTACCAGTGG ATCCAGGGCC GGCTCAAGGC AGAGCAGATG 
GAGGAGCTGG CAACCTCATT CAGCTCCCTG CTGGCATATG GCCTCTCCCT CATCCGGAGG 
TTCCGGTCTG TCTTCCCCTT ATCTGTCTCG GACTCCCCAG CTCGGCTACA GTCTCTCCTC 
AGGGTCCTGG TACAGATGTG CAAGATGAAG GCCTTTAGAG AACTGTGCCC TGACAGTGGC 
CCACTGCCCC ACCTGGTGAC TGAGGCGCTG CGGGCTGGCA CCGTTGAATG GTTCCACCTG 
AAGCAGCAGC ACCATCAGCC AATGGTGCAG GGCATGCTGG AGGCAGGCAA GGCCTTGCTG 
AGCCTGGTCC AAGACATCAT CGGTGACCTG CACCAGTGCC AGCGCACGTG GAACAAGATC 
TTCCACAATG CCCTTAAGAT CGACCTCTTC GCCATGGCTT TCCTGGAGCT GCAGTGGCTG 
GTGGCCAAGC GCGTGCAGGA CCACACGGCC GCGGCGTTGA GAGGCCTCGT GTCCCCAGAG 
ATGGGAGAGA GTTTGTTCCA GCTCTACATC ATCCTGAAGG AGCTCTGCCA GCTGGGCCCC 
GCCCCCTCGG AGAGGGATGG AGTCCTGGCC CTGGATGGCT TCCACCGCTG GTTCCAGCCT 
GCCATCCCTT CCTGGCTGCA GAAGACATAC AGCGTGGCCT TGGCGCGGGT GCAGCGCGCT 
GTGCAGATGG ACAAGCTGGT GCCCCTGGGT GAACTGACCA AGCATAGCAC ATCGGCCGTG 
GATCTGTCCA CCTGCTTTGC TCAGATCAGC CACACTGCCC GGCAGCTGGA CTGGCCCGAC 
CCAGAGGAGG CCTTCATGAT CACAGTCAAG TTTGTGGAGG ACACCTGTCG GCTGGCCCTG 
GTGTACTGCA GCCTGATAAA GGCCCGGGCC CGAGAGCTCT CTGCAGGCCA GAAGGACCAG 
GGCCAGGCAG CCAATATGCT GTGCGTGGTG GTGAACGACA TGGAGCAGCT GCGGCTGGTG 
ATCGGCAGGC TGCCCACCCA GCTGGCATGG GAGGCGCTGG AGCAGCGAGT AGGGGCTGTG 
CTGGAGCAGG GGCAGCTGCA GAACACGCTG CATGCCCAGC TGCAGGGTGC GCTGGCGGGG 
CTGGGCCACG AGATCCGCAC CGGCGTCCGC ACCCTTGCGG AGCAGCTGGA GGTGGGCATC 
GCCAAGCACA TCCAGAACCT CGTGGGCGTC AGGGAGTCCA TCTTGCCTGA GGATGCCATT 
CTGCCCCTGA TGAAATTCCT GGAGGTGGAG CTCTGCTACA TGAACACCAA CTTGGTGCAG 
GAGAACTTCA GCAGCCTTCT GACCCTGCTC TGGACCCACA CGCTCGAGGT GATGGTTGAG 
GGTGCCACCT CCCAGCGGAG CTGCCCCCTG GCCTCTAGCA GGCTGAAGAT TGCGCTGCAG 
AACCTGGAGA TCTGCTTCTA CGCAGAGGGC TGTGGCCTGC AGCCCGAGGC CCTGCACACG 
GCCACCTTCC AGGCTTTGCA GAGGGACTTA GAGCTGCAGG CGGCCTCCAG CCGGGAGCTC 
ATCCAGAAGT ACTTCTGCAA CCGCATCCAG CAGCAGGCGG AAACCACCTC TGAGGAGCTC 
GGGGCGATCA CCGTCAAGGC CTCCTACCGC GCCTCTGAGC AGAAGCTGCA TGTGGAGGTG 
CTCAGCGCCT CCAGCCTGCT GCCCCTGGAC TCCAATGGCT CCAGCGACCC CTTTGTTCAG 
CTGACCTTGG AGCCCAGGCA CGAGTTTCCT GAGCTGGCCC CCCGGGAAAC CCAAAAGCAC 
AAGAAGGACC TTCACCCGCT GTTTGATGAG ACCTTTGAAT TCCTGGTGCC TGCTGAGCCG 
TGCCAGAAGG ACGGGGCGTG CCTCCTGCTC ACAGTGCTGG ACCATGACAC ATTGGGGGCC 
GACGACCTGG AGGGCGAGGC CTTTCTGCCG CTGCGCTCGG TGCTGGGCCT CAGTGGGACG 
GAGGAGCCTG GGGAGGTGCC TCAGAGCCGC CTGCCCCTCA CCTACCCCGC ACCCAATGGG 
GACCCAATCC TGCAGCTTCT GGAGAGCCGG AAGGGTGATC GTGAGGCCCA GGTGTTTGTG 
AAGCTGCGGC GGCAGCGAGC CAAGCAGGCC TCCCAGCATG CCGTGCGGCT GGGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015445264.1
CDS	333..3572
Translation	MDLSHSRPEEDEGDLGARPSGGLLGEQIEAPSHHFSPEERALLYEEALYTVLHRLGKPEPGHVSEASELLRYLQEAFHVEPEEHQRMLRQVQALEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGTLEGSPGSRRRQKAVVKHTIPEEQTHRTQVISQTLNPVWDETFILEFEDIRNSSFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPCTETYPDRGQCHLQFQLIHKRRATGASRSQPSYTVHLHLLQQLVSHEVTQHQAGSTSWDGLLSPQAATILFLHATQKDLSDFHQSMAQWLAYSRVYQSLEFPSSCLLHPITSIEYQWIQGRLKAEQMEELATSFSSLLAYGLSLIRRFRSVFPLSVSDSPARLQSLLRVLVQMCKMKAFRELCPDSGPLPHLVTEALRAGTVEWFHLKQQHHQPMVQGMLEAGKALLSLVQDIIGDLHQCQRTWNKIFHNALKIDLFAMAFLELQWLVAKRVQDHTAAALRGLVSPEMGESLFQLYIILKELCQLGPAPSERDGVLALDGFHRWFQPAIPSWLQKTYSVALARVQRAVQMDKLVPLGELTKHSTSAVDLSTCFAQISHTARQLDWPDPEEAFMITVKFVEDTCRLALVYCSLIKARARELSAGQKDQGQAANMLCVVVNDMEQLRLVIGRLPTQLAWEALEQRVGAVLEQGQLQNTLHAQLQGALAGLGHEIRTGVRTLAEQLEVGIAKHIQNLVGVRESILPEDAILPLMKFLEVELCYMNTNLVQENFSSLLTLLWTHTLEVMVEGATSQRSCPLASSRLKIALQNLEICFYAEGCGLQPEALHTATFQALQRDLELQAASSRELIQKYFCNRIQQQAETTSEELGAITVKASYRASEQKLHVEVLSASSLLPLDSNGSSDPFVQLTLEPRHEFPELAPRETQKHKKDLHPLFDETFEFLVPAEPCQKDGACLLLTVLDHDTLGADDLEGEAFLPLRSVLGLSGTEEPGEVPQSRLPLTYPAPNGDPILQLLESRKGDREAQVFVKLRRQRAKQASQHAVRLGQ

Target ORF information:

RefSeq Version	XM_015589778.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto unc-13 homolog D (C. elegans) (UNC13D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015589778.1

ORF Insert Sequence:

ATGGACCTCA GCCACAGCAG GCCAGAGGAG GACGAGGGCG ACCTGGGGGC CAGACCCTCA 
GGAGGTCTGC TGGGGGAACA GATCGAGGCT CCATCCCACC ACTTCTCCCC TGAGGAGCGG 
GCCCTGCTCT ACGAGGAAGC CCTCTACACT GTCCTGCACC GCCTGGGAAA GCCCGAGCCC 
GGCCATGTGA GCGAGGCGTC CGAGCTGCTG CGCTACCTGC AGGAGGCCTT CCACGTGGAG 
CCCGAGGAAC ACCAGCGGAT GCTGCGGCAG GTGCAGGCCC TCGAGAAGCC AATATTTTGT 
CTGAAGGCAA CGGTGAAACA AGCCAAGGGC ATTCTGGGCA AGGATGTCAG CGGGTTCAGT 
GACCCCTACT GCCTGCTGGG CATTGAGCAG GGAGTGGGCA CGCTGGAGGG CAGCCCCGGG 
TCTCGGCGGC GGCAGAAGGC TGTGGTGAAG CACACCATCC CGGAGGAGCA GACACACCGC 
ACCCAGGTCA TCAGCCAGAC ACTCAACCCC GTCTGGGACG AGACCTTCAT CCTGGAGTTT 
GAGGACATAA GAAATTCGAG TTTTCATCTG GACATGTGGG ACCTGGACAC CGTGGAGTCT 
GTCAGGCAGA AGCTTGGGGA GCTCACAGAT CTGCACGGGC TGCGCAGGAT CTTTAAGGAG 
GCCCGGAAGG ACAAAGGCCA GGACGACTTC CTGGGAAACG TGGTGCTGCG GCTGCAGGAC 
CTTCGCTGCC GGGAGGATCA GTGGTACCCT CTGGAGCCCT GCACTGAGAC CTACCCAGAC 
CGTGGCCAGT GCCACCTCCA GTTCCAGCTC ATCCACAAGC GGAGAGCCAC TGGGGCCAGC 
CGCTCCCAGC CCAGCTACAC GGTGCACCTC CACCTGCTGC AACAGCTCGT GTCCCACGAG 
GTCACCCAGC ACCAGGCAGG CAGCACCTCC TGGGATGGGC TGCTGAGTCC TCAGGCTGCC 
ACCATCCTGT TTCTCCATGC CACGCAGAAG GACCTGTCCG ACTTCCACCA GTCCATGGCG 
CAGTGGCTGG CCTACAGCCG TGTCTACCAG AGCCTGGAGT TCCCCAGCAG CTGCCTCCTG 
CACCCTATCA CCAGCATCGA GTACCAGTGG ATCCAGGGCC GGCTCAAGGC AGAGCAGATG 
GAGGAGCTGG CAACCTCATT CAGCTCCCTG CTGGCATATG GCCTCTCCCT CATCCGGAGG 
TTCCGGTCTG TCTTCCCCTT ATCTGTCTCG GACTCCCCAG CTCGGCTACA GTCTCTCCTC 
AGGGTCCTGG TACAGATGTG CAAGATGAAG GCCTTTAGAG AACTGTGCCC TGACAGTGGC 
CCACTGCCCC ACCTGGTGAC TGAGGCGCTG CGGGCTGGCA CCGTTGAATG GTTCCACCTG 
AAGCAGCAGC ACCATCAGCC AATGGTGCAG GGCATGCTGG AGGCAGGCAA GGCCTTGCTG 
AGCCTGGTCC AAGACATCAT CGGTGACCTG CACCAGTGCC AGCGCACGTG GAACAAGATC 
TTCCACAATG CCCTTAAGAT CGACCTCTTC GCCATGGCTT TCCTGGAGCT GCAGTGGCTG 
GTGGCCAAGC GCGTGCAGGA CCACACGGCC GCGGCGTTGA GAGGCCTCGT GTCCCCAGAG 
ATGGGAGAGA GTTTGTTCCA GCTCTACATC ATCCTGAAGG AGCTCTGCCA GCTGGGCCCC 
GCCCCCTCGG AGAGGGATGG AGTCCTGGCC CTGGATGGCT TCCACCGCTG GTTCCAGCCT 
GCCATCCCTT CCTGGCTGCA GAAGACATAC AGCGTGGCCT TGGCGCGGGT GCAGCGCGCT 
GTGCAGATGG ACAAGCTGGT GCCCCTGGGT GAACTGACCA AGCATAGCAC ATCGGCCGTG 
GATCTGTCCA CCTGCTTTGC TCAGATCAGC CACACTGCCC GGCAGCTGGA CTGGCCCGAC 
CCAGAGGAGG CCTTCATGAT CACAGTCAAG TTTGTGGAGG ACACCTGTCG GCTGGCCCTG 
GTGTACTGCA GCCTGATAAA GGCCCGGGCC CGAGAGCTCT CTGCAGGCCA GAAGGACCAG 
GGCCAGGCAG CCAATATGCT GTGCGTGGTG GTGAACGACA TGGAGCAGCT GCGGCTGGTG 
ATCGGCAGGC TGCCCACCCA GCTGGCATGG GAGGCGCTGG AGCAGCGAGT AGGGGCTGTG 
CTGGAGCAGG GGCAGCTGCA GAACACGCTG CATGCCCAGC TGCAGGGTGC GCTGGCGGGG 
CTGGGCCACG AGATCCGCAC CGGCGTCCGC ACCCTTGCGG AGCAGCTGGA GGTGGGCATC 
GCCAAGCACA TCCAGAACCT CGTGGGCGTC AGGGAGTCCA TCTTGCCTGA GGATGCCATT 
CTGCCCCTGA TGAAATTCCT GGAGGTGGAG CTCTGCTACA TGAACACCAA CTTGGTGCAG 
GAGAACTTCA GCAGCCTTCT GACCCTGCTC TGGACCCACA CGCTCGAGGT GATGGTTGAG 
GGTGCCACCT CCCAGCGGAG CTGCCCCCTG GCCTCTAGCA GGCTGAAGAT TGCGCTGCAG 
AACCTGGAGA TCTGCTTCTA CGCAGAGGGC TGTGGCCTGC AGCCCGAGGC CCTGCACACG 
GCCACCTTCC AGGCTTTGCA GAGGGACTTA GAGCTGCAGG CGGCCTCCAG CCGGGAGCTC 
ATCCAGAAGT ACTTCTGCAA CCGCATCCAG CAGCAGGCGG AAACCACCTC TGAGGAGCTC 
GGGGCGATCA CCGTCAAGGC CTCCTACCGC GCCTCTGAGC AGAAGCTGCA TGTGGAGGTG 
CTCAGCGCCT CCAGCCTGCT GCCCCTGGAC TCCAATGGCT CCAGCGACCC CTTTGTTCAG 
CTGACCTTGG AGCCCAGGCA CGAGTTTCCT GAGCTGGCCC CCCGGGAAAC CCAAAAGCAC 
AAGAAGGACC TTCACCCGCT GTTTGATGAG ACCTTTGAAT TCCTGGTGCC TGCTGAGCCG 
TGCCAGAAGG ACGGGGCGTG CCTCCTGCTC ACAGTGCTGG ACCATGACAC ATTGGGGGCC 
GACGACCTGG AGGGCGAGGC CTTTCTGCCG CTGCGCTCGG TGCTGGGCCT CAGTGGGACG 
GAGGAGCCTG GGGAGGTGCC TCAGAGCCGC CTGCCCCTCA CCTACCCCGC ACCCAATGGG 
GACCCAATCC TGCAGCTTCT GGAGAGCCGG AAGGGTGATC GTGAGGCCCA GGTGTTTGTG 
AAGCTGCGGC GGCAGCGAGC CAAGCAGGCC TCCCAGCATG CCGTGCGGCT GGGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC13D ( XM_015589778.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015445264.1 Pteropus alecto unc-13 homolog D (UNC13D), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPt15192
Clone ID Related Accession (Same CDS sequence)	XM_015589778.1 , XM_015589778.2
Accession Version	XM_015589778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	protein unc-13 homolog D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006436283.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015589778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTCA GCCACAGCAG GCCAGAGGAG GACGAGGGCG ACCTGGGGGC CAGACCCTCA 
GGAGGTCTGC TGGGGGAACA GATCGAGGCT CCATCCCACC ACTTCTCCCC TGAGGAGCGG 
GCCCTGCTCT ACGAGGAAGC CCTCTACACT GTCCTGCACC GCCTGGGAAA GCCCGAGCCC 
GGCCATGTGA GCGAGGCGTC CGAGCTGCTG CGCTACCTGC AGGAGGCCTT CCACGTGGAG 
CCCGAGGAAC ACCAGCGGAT GCTGCGGCAG GTGCAGGCCC TCGAGAAGCC AATATTTTGT 
CTGAAGGCAA CGGTGAAACA AGCCAAGGGC ATTCTGGGCA AGGATGTCAG CGGGTTCAGT 
GACCCCTACT GCCTGCTGGG CATTGAGCAG GGAGTGGGCA CGCTGGAGGG CAGCCCCGGG 
TCTCGGCGGC GGCAGAAGGC TGTGGTGAAG CACACCATCC CGGAGGAGCA GACACACCGC 
ACCCAGGTCA TCAGCCAGAC ACTCAACCCC GTCTGGGACG AGACCTTCAT CCTGGAGTTT 
GAGGACATAA GAAATTCGAG TTTTCATCTG GACATGTGGG ACCTGGACAC CGTGGAGTCT 
GTCAGGCAGA AGCTTGGGGA GCTCACAGAT CTGCACGGGC TGCGCAGGAT CTTTAAGGAG 
GCCCGGAAGG ACAAAGGCCA GGACGACTTC CTGGGAAACG TGGTGCTGCG GCTGCAGGAC 
CTTCGCTGCC GGGAGGATCA GTGGTACCCT CTGGAGCCCT GCACTGAGAC CTACCCAGAC 
CGTGGCCAGT GCCACCTCCA GTTCCAGCTC ATCCACAAGC GGAGAGCCAC TGGGGCCAGC 
CGCTCCCAGC CCAGCTACAC GGTGCACCTC CACCTGCTGC AACAGCTCGT GTCCCACGAG 
GTCACCCAGC ACCAGGCAGG CAGCACCTCC TGGGATGGGC TGCTGAGTCC TCAGGCTGCC 
ACCATCCTGT TTCTCCATGC CACGCAGAAG GACCTGTCCG ACTTCCACCA GTCCATGGCG 
CAGTGGCTGG CCTACAGCCG TGTCTACCAG AGCCTGGAGT TCCCCAGCAG CTGCCTCCTG 
CACCCTATCA CCAGCATCGA GTACCAGTGG ATCCAGGGCC GGCTCAAGGC AGAGCAGATG 
GAGGAGCTGG CAACCTCATT CAGCTCCCTG CTGGCATATG GCCTCTCCCT CATCCGGAGG 
TTCCGGTCTG TCTTCCCCTT ATCTGTCTCG GACTCCCCAG CTCGGCTACA GTCTCTCCTC 
AGGGTCCTGG TACAGATGTG CAAGATGAAG GCCTTTAGAG AACTGTGCCC TGACAGTGGC 
CCACTGCCCC ACCTGGTGAC TGAGGCGCTG CGGGCTGGCA CCGTTGAATG GTTCCACCTG 
AAGCAGCAGC ACCATCAGCC AATGGTGCAG GGCATGCTGG AGGCAGGCAA GGCCTTGCTG 
AGCCTGGTCC AAGACATCAT CGGTGACCTG CACCAGTGCC AGCGCACGTG GAACAAGATC 
TTCCACAATG CCCTTAAGAT CGACCTCTTC GCCATGGCTT TCCTGGAGCT GCAGTGGCTG 
GTGGCCAAGC GCGTGCAGGA CCACACGGCC GCGGCGTTGA GAGGCCTCGT GTCCCCAGAG 
ATGGGAGAGA GTTTGTTCCA GCTCTACATC ATCCTGAAGG AGCTCTGCCA GCTGGGCCCC 
GCCCCCTCGG AGAGGGATGG AGTCCTGGCC CTGGATGGCT TCCACCGCTG GTTCCAGCCT 
GCCATCCCTT CCTGGCTGCA GAAGACATAC AGCGTGGCCT TGGCGCGGGT GCAGCGCGCT 
GTGCAGATGG ACAAGCTGGT GCCCCTGGGT GAACTGACCA AGCATAGCAC ATCGGCCGTG 
GATCTGTCCA CCTGCTTTGC TCAGATCAGC CACACTGCCC GGCAGCTGGA CTGGCCCGAC 
CCAGAGGAGG CCTTCATGAT CACAGTCAAG TTTGTGGAGG ACACCTGTCG GCTGGCCCTG 
GTGTACTGCA GCCTGATAAA GGCCCGGGCC CGAGAGCTCT CTGCAGGCCA GAAGGACCAG 
GGCCAGGCAG CCAATATGCT GTGCGTGGTG GTGAACGACA TGGAGCAGCT GCGGCTGGTG 
ATCGGCAGGC TGCCCACCCA GCTGGCATGG GAGGCGCTGG AGCAGCGAGT AGGGGCTGTG 
CTGGAGCAGG GGCAGCTGCA GAACACGCTG CATGCCCAGC TGCAGGGTGC GCTGGCGGGG 
CTGGGCCACG AGATCCGCAC CGGCGTCCGC ACCCTTGCGG AGCAGCTGGA GGTGGGCATC 
GCCAAGCACA TCCAGAACCT CGTGGGCGTC AGGGAGTCCA TCTTGCCTGA GGATGCCATT 
CTGCCCCTGA TGAAATTCCT GGAGGTGGAG CTCTGCTACA TGAACACCAA CTTGGTGCAG 
GAGAACTTCA GCAGCCTTCT GACCCTGCTC TGGACCCACA CGCTCGAGGT GATGGTTGAG 
GGTGCCACCT CCCAGCGGAG CTGCCCCCTG GCCTCTAGCA GGCTGAAGAT TGCGCTGCAG 
AACCTGGAGA TCTGCTTCTA CGCAGAGGGC TGTGGCCTGC AGCCCGAGGC CCTGCACACG 
GCCACCTTCC AGGCTTTGCA GAGGGACTTA GAGCTGCAGG CGGCCTCCAG CCGGGAGCTC 
ATCCAGAAGT ACTTCTGCAA CCGCATCCAG CAGCAGGCGG AAACCACCTC TGAGGAGCTC 
GGGGCGATCA CCGTCAAGGC CTCCTACCGC GCCTCTGAGC AGAAGCTGCA TGTGGAGGTG 
CTCAGCGCCT CCAGCCTGCT GCCCCTGGAC TCCAATGGCT CCAGCGACCC CTTTGTTCAG 
CTGACCTTGG AGCCCAGGCA CGAGTTTCCT GAGCTGGCCC CCCGGGAAAC CCAAAAGCAC 
AAGAAGGACC TTCACCCGCT GTTTGATGAG ACCTTTGAAT TCCTGGTGCC TGCTGAGCCG 
TGCCAGAAGG ACGGGGCGTG CCTCCTGCTC ACAGTGCTGG ACCATGACAC ATTGGGGGCC 
GACGACCTGG AGGGCGAGGC CTTTCTGCCG CTGCGCTCGG TGCTGGGCCT CAGTGGGACG 
GAGGAGCCTG GGGAGGTGCC TCAGAGCCGC CTGCCCCTCA CCTACCCCGC ACCCAATGGG 
GACCCAATCC TGCAGCTTCT GGAGAGCCGG AAGGGTGATC GTGAGGCCCA GGTGTTTGTG 
AAGCTGCGGC GGCAGCGAGC CAAGCAGGCC TCCCAGCATG CCGTGCGGCT GGGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015445264.1
CDS	398..3637
Translation	MDLSHSRPEEDEGDLGARPSGGLLGEQIEAPSHHFSPEERALLYEEALYTVLHRLGKPEPGHVSEASELLRYLQEAFHVEPEEHQRMLRQVQALEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGTLEGSPGSRRRQKAVVKHTIPEEQTHRTQVISQTLNPVWDETFILEFEDIRNSSFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPCTETYPDRGQCHLQFQLIHKRRATGASRSQPSYTVHLHLLQQLVSHEVTQHQAGSTSWDGLLSPQAATILFLHATQKDLSDFHQSMAQWLAYSRVYQSLEFPSSCLLHPITSIEYQWIQGRLKAEQMEELATSFSSLLAYGLSLIRRFRSVFPLSVSDSPARLQSLLRVLVQMCKMKAFRELCPDSGPLPHLVTEALRAGTVEWFHLKQQHHQPMVQGMLEAGKALLSLVQDIIGDLHQCQRTWNKIFHNALKIDLFAMAFLELQWLVAKRVQDHTAAALRGLVSPEMGESLFQLYIILKELCQLGPAPSERDGVLALDGFHRWFQPAIPSWLQKTYSVALARVQRAVQMDKLVPLGELTKHSTSAVDLSTCFAQISHTARQLDWPDPEEAFMITVKFVEDTCRLALVYCSLIKARARELSAGQKDQGQAANMLCVVVNDMEQLRLVIGRLPTQLAWEALEQRVGAVLEQGQLQNTLHAQLQGALAGLGHEIRTGVRTLAEQLEVGIAKHIQNLVGVRESILPEDAILPLMKFLEVELCYMNTNLVQENFSSLLTLLWTHTLEVMVEGATSQRSCPLASSRLKIALQNLEICFYAEGCGLQPEALHTATFQALQRDLELQAASSRELIQKYFCNRIQQQAETTSEELGAITVKASYRASEQKLHVEVLSASSLLPLDSNGSSDPFVQLTLEPRHEFPELAPRETQKHKKDLHPLFDETFEFLVPAEPCQKDGACLLLTVLDHDTLGADDLEGEAFLPLRSVLGLSGTEEPGEVPQSRLPLTYPAPNGDPILQLLESRKGDREAQVFVKLRRQRAKQASQHAVRLGQ

Target ORF information:

RefSeq Version	XM_015589778.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto unc-13 homolog D (UNC13D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015589778.2

ORF Insert Sequence:

ATGGACCTCA GCCACAGCAG GCCAGAGGAG GACGAGGGCG ACCTGGGGGC CAGACCCTCA 
GGAGGTCTGC TGGGGGAACA GATCGAGGCT CCATCCCACC ACTTCTCCCC TGAGGAGCGG 
GCCCTGCTCT ACGAGGAAGC CCTCTACACT GTCCTGCACC GCCTGGGAAA GCCCGAGCCC 
GGCCATGTGA GCGAGGCGTC CGAGCTGCTG CGCTACCTGC AGGAGGCCTT CCACGTGGAG 
CCCGAGGAAC ACCAGCGGAT GCTGCGGCAG GTGCAGGCCC TCGAGAAGCC AATATTTTGT 
CTGAAGGCAA CGGTGAAACA AGCCAAGGGC ATTCTGGGCA AGGATGTCAG CGGGTTCAGT 
GACCCCTACT GCCTGCTGGG CATTGAGCAG GGAGTGGGCA CGCTGGAGGG CAGCCCCGGG 
TCTCGGCGGC GGCAGAAGGC TGTGGTGAAG CACACCATCC CGGAGGAGCA GACACACCGC 
ACCCAGGTCA TCAGCCAGAC ACTCAACCCC GTCTGGGACG AGACCTTCAT CCTGGAGTTT 
GAGGACATAA GAAATTCGAG TTTTCATCTG GACATGTGGG ACCTGGACAC CGTGGAGTCT 
GTCAGGCAGA AGCTTGGGGA GCTCACAGAT CTGCACGGGC TGCGCAGGAT CTTTAAGGAG 
GCCCGGAAGG ACAAAGGCCA GGACGACTTC CTGGGAAACG TGGTGCTGCG GCTGCAGGAC 
CTTCGCTGCC GGGAGGATCA GTGGTACCCT CTGGAGCCCT GCACTGAGAC CTACCCAGAC 
CGTGGCCAGT GCCACCTCCA GTTCCAGCTC ATCCACAAGC GGAGAGCCAC TGGGGCCAGC 
CGCTCCCAGC CCAGCTACAC GGTGCACCTC CACCTGCTGC AACAGCTCGT GTCCCACGAG 
GTCACCCAGC ACCAGGCAGG CAGCACCTCC TGGGATGGGC TGCTGAGTCC TCAGGCTGCC 
ACCATCCTGT TTCTCCATGC CACGCAGAAG GACCTGTCCG ACTTCCACCA GTCCATGGCG 
CAGTGGCTGG CCTACAGCCG TGTCTACCAG AGCCTGGAGT TCCCCAGCAG CTGCCTCCTG 
CACCCTATCA CCAGCATCGA GTACCAGTGG ATCCAGGGCC GGCTCAAGGC AGAGCAGATG 
GAGGAGCTGG CAACCTCATT CAGCTCCCTG CTGGCATATG GCCTCTCCCT CATCCGGAGG 
TTCCGGTCTG TCTTCCCCTT ATCTGTCTCG GACTCCCCAG CTCGGCTACA GTCTCTCCTC 
AGGGTCCTGG TACAGATGTG CAAGATGAAG GCCTTTAGAG AACTGTGCCC TGACAGTGGC 
CCACTGCCCC ACCTGGTGAC TGAGGCGCTG CGGGCTGGCA CCGTTGAATG GTTCCACCTG 
AAGCAGCAGC ACCATCAGCC AATGGTGCAG GGCATGCTGG AGGCAGGCAA GGCCTTGCTG 
AGCCTGGTCC AAGACATCAT CGGTGACCTG CACCAGTGCC AGCGCACGTG GAACAAGATC 
TTCCACAATG CCCTTAAGAT CGACCTCTTC GCCATGGCTT TCCTGGAGCT GCAGTGGCTG 
GTGGCCAAGC GCGTGCAGGA CCACACGGCC GCGGCGTTGA GAGGCCTCGT GTCCCCAGAG 
ATGGGAGAGA GTTTGTTCCA GCTCTACATC ATCCTGAAGG AGCTCTGCCA GCTGGGCCCC 
GCCCCCTCGG AGAGGGATGG AGTCCTGGCC CTGGATGGCT TCCACCGCTG GTTCCAGCCT 
GCCATCCCTT CCTGGCTGCA GAAGACATAC AGCGTGGCCT TGGCGCGGGT GCAGCGCGCT 
GTGCAGATGG ACAAGCTGGT GCCCCTGGGT GAACTGACCA AGCATAGCAC ATCGGCCGTG 
GATCTGTCCA CCTGCTTTGC TCAGATCAGC CACACTGCCC GGCAGCTGGA CTGGCCCGAC 
CCAGAGGAGG CCTTCATGAT CACAGTCAAG TTTGTGGAGG ACACCTGTCG GCTGGCCCTG 
GTGTACTGCA GCCTGATAAA GGCCCGGGCC CGAGAGCTCT CTGCAGGCCA GAAGGACCAG 
GGCCAGGCAG CCAATATGCT GTGCGTGGTG GTGAACGACA TGGAGCAGCT GCGGCTGGTG 
ATCGGCAGGC TGCCCACCCA GCTGGCATGG GAGGCGCTGG AGCAGCGAGT AGGGGCTGTG 
CTGGAGCAGG GGCAGCTGCA GAACACGCTG CATGCCCAGC TGCAGGGTGC GCTGGCGGGG 
CTGGGCCACG AGATCCGCAC CGGCGTCCGC ACCCTTGCGG AGCAGCTGGA GGTGGGCATC 
GCCAAGCACA TCCAGAACCT CGTGGGCGTC AGGGAGTCCA TCTTGCCTGA GGATGCCATT 
CTGCCCCTGA TGAAATTCCT GGAGGTGGAG CTCTGCTACA TGAACACCAA CTTGGTGCAG 
GAGAACTTCA GCAGCCTTCT GACCCTGCTC TGGACCCACA CGCTCGAGGT GATGGTTGAG 
GGTGCCACCT CCCAGCGGAG CTGCCCCCTG GCCTCTAGCA GGCTGAAGAT TGCGCTGCAG 
AACCTGGAGA TCTGCTTCTA CGCAGAGGGC TGTGGCCTGC AGCCCGAGGC CCTGCACACG 
GCCACCTTCC AGGCTTTGCA GAGGGACTTA GAGCTGCAGG CGGCCTCCAG CCGGGAGCTC 
ATCCAGAAGT ACTTCTGCAA CCGCATCCAG CAGCAGGCGG AAACCACCTC TGAGGAGCTC 
GGGGCGATCA CCGTCAAGGC CTCCTACCGC GCCTCTGAGC AGAAGCTGCA TGTGGAGGTG 
CTCAGCGCCT CCAGCCTGCT GCCCCTGGAC TCCAATGGCT CCAGCGACCC CTTTGTTCAG 
CTGACCTTGG AGCCCAGGCA CGAGTTTCCT GAGCTGGCCC CCCGGGAAAC CCAAAAGCAC 
AAGAAGGACC TTCACCCGCT GTTTGATGAG ACCTTTGAAT TCCTGGTGCC TGCTGAGCCG 
TGCCAGAAGG ACGGGGCGTG CCTCCTGCTC ACAGTGCTGG ACCATGACAC ATTGGGGGCC 
GACGACCTGG AGGGCGAGGC CTTTCTGCCG CTGCGCTCGG TGCTGGGCCT CAGTGGGACG 
GAGGAGCCTG GGGAGGTGCC TCAGAGCCGC CTGCCCCTCA CCTACCCCGC ACCCAATGGG 
GACCCAATCC TGCAGCTTCT GGAGAGCCGG AAGGGTGATC GTGAGGCCCA GGTGTTTGTG 
AAGCTGCGGC GGCAGCGAGC CAAGCAGGCC TCCCAGCATG CCGTGCGGCT GGGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

UNC13D cDNA ORF clone, Pteropus alecto(black flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム