NNT cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	NNT
Entrez Gene ID	102895403
Full Name	nicotinamide nucleotide transhydrogenase
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006912445.2	XP_006912507.1	NAD(P) transhydrogenase, mitochondrial
XM_025047215.1	XP_024902983.1	NAD(P) transhydrogenase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following NNT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NNT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt15298	XM_025047215.1 Latest version!	Pteropus alecto nicotinamide nucleotide transhydrogenase (NNT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPt15298	XM_006912445.2 Latest version!	Pteropus alecto nicotinamide nucleotide transhydrogenase (NNT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NNT ( XM_006912445.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006912507.1 Pteropus alecto nicotinamide nucleotide transhydrogenase (NNT), mRNA.

Price & Availability↑

CloneID	OPt15298
Clone ID Related Accession (Same CDS sequence)	XM_006912445.2 , XM_025047215.1
Accession Version	XM_006912445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	NAD(P) transhydrogenase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006436284.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006912445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGG CGAACCTACT GAAAACAGTG GTGACTGGCT GTTCGTGTCC TTTACTTAGC 
AATTTGGGGT CCTATAAGGT TCTACCTGGG AAGAAGGATG TTTTACGAAC ATTTCATACT 
CATCGGGCAC TGTGTAAATC CTCTGTAAAA CCAGGAATTC CATATAAACA ATTGACGGTT 
GGACTCCCCA AGGAGATTTT CCAAAATGAG AAGCGAGTGG CTTTGTCTCC TGCTGGCGTT 
CAGATCCTGG TCAAGCAGGG TTTTAATGTT GCTGTGGAAT CGGGTGCGGG AGAAGCTTCT 
AAGTTCTCAG ATGATCACTA CAGAGCGGCA GGTGCCCAAA TCCAAGGGAC GAAGGAAGTG 
CTGGCTTCCG ATTTGGTGGT CAAAGTGCGA GCCCCCATGA TTAATCCAGT ATTAAATAGT 
CATGAAGCGG ACCTTTTAAA GACATCGGGA ACACTTATTA GTTTTATTTA TCCAGCCCAA 
AATCCAGACT TGCTAAATAA ACTTTCCAAA AGAAAAACTA CAGTTTTGGC AATGGACCAG 
GTTCCAAGAG TCACAATTGC TCAGGGATAT GATGCGCTAA GCTCCATGGC CAACATTGCT 
GGGTATAAGG CTGTTGTACT AGCAGCAAAT CATTTTGGAC GATTTTTTAC TGGTCAGATC 
ACAGCTGCTG GAAAGGTTCC TCCAGCTAAG ATTCTGATAG TTGGCGGTGG AGTTGCTGGG 
CTTGCTTCTG CAGGCGCAGC CAAGTCAATG GGTGCAATTG TTCGAGGATT TGACACGAGA 
GCTGCAGCTT TGGAGCAGTT CAAATCTCTT GGTGCTGAGC CCTTAGAGGT AGACTTGAAG 
GAATCTGGTG AGGGACAAGG CGGATATGCG AAAGAGATGT CTAAAGAGTT CATTGAAGCT 
GAAATGAAGC TCTTTGCTCA GCAGTGCAAG GAGGTGGACA TCCTTATCAG CACAGCACTT 
ATTCCAGGTA AAAAAGCTCC AGTTTTATTT AGTAAAGAAA TGATCGAGTC AATGAAGGAA 
GGCTCAGTTG TTGTGGATTT AGCTGCTGAG GCTGGCGGAA ACTTTGAAAC CACTAAGCCA 
GGAGAACTGT ATGTTCATAA GGGAATTACT CACATAGGCT ATACGGACCT TCCCAGCCGA 
ATGGCTACTC AAGCCAGCAC CCTGTATTCC AATAACATCA CCAAGCTCCT GAAGGCCATC 
AGCCCTGACA AAGATAATTT TTATTTTGAA GTGAAAGATG ACTTTGACTT TGGTACAATG 
GGTCATGTCA TTAGAGGAAC TGTAGTGATG AAGGATGGTG AAGTGATTTT CCCTGCTCCC 
ACACCTAAAA ATATTCCTCA AGGTGCCCCA GTAAAACAGA AGACAGTGGC TGAGCTGGAA 
GCTGAAAAAG CGGCTACTAT TACACCCTTC AGGAAAACGA TGACCACGGC TTCTGCATAT 
ACAGCAGGTG AGGATCCCAT TNNNGGTTTG GGCATTGCAG CTCCCAATTT AGCATTTTCC 
CAGATGGTAA CCACCTTTGG CTTGGCCGGC ATTGTGGGCT ACCACACAGT CTGGGGAGTG 
ACTCCTGCCC TCCACTCACC ACTGATGTCT GTGACTAATG CAATCTCAGG GTTGACTGCA 
GTTGGTGGGT TGGCACTGAT GGGAGGACAT TTGTATCCTT CCACAACTTC TGAGGGCCTT 
GCTGCTCTTG CTACATTCAT ATCCTCAGTC AACATTGCAG GTGGCTTCCT GGTGACTCAG 
AGAATGCTGG ACATGTTTAA GCGTCCCACC GATCCCCCAG AGTACAATTA TCTGTATCTG 
CTCCCTGCTG GTACCTTTGT TGGAGGATAT TTATCTGCCC TCTACAGTGG TTATAACATT 
GAGCAAATCA TGTACCTAGG CTCAGGACTG TGCTGTGTTG GTGCCCTGGC TGGCCTTTCC 
ACCCAGGGAA CAGCTCGTCT TGGCAATGCA CTGGGAATGA TTGGGGTGTC TGGAGGCCTG 
GCAGCCACCC TTGGAGGCTT AAACCCGAGC CCAGAATTGT TAGCTCAGAT GTCTGGAGCC 
ATGGCTTTGG GCGGTACCAT TGGATTGACA ATTGCCAAGC GCATCCAGAT TTCTGATTTA 
CCTCAGTTAG TTGCTGCTTT CCACAGTTTA GTAGGCTTGG CAGCTGTGCT TACTTGCATA 
GCTGAGTATA TTATAGAATA TCCACATTTT GCTATGGATC CAGCAGCAAA TCTCACCAAG 
ATCGTGGCCT ACCTCGGTAC TTACATCGGT GGTGTCACAT TTAGTGGGTC TCTTATTGCC 
TATGGAAAAT TGCAGGGTAT TCTGAAATCT GCCCCTCTCC TGCTCCCCGG AAGGCATGCA 
CTCAATGCAG GCCTGCTGGC TGCTAGTGTG GGTGGAATGA TCCCATTCAT GATGGACCCC 
AGCTTTACCA CGGGCATTAT CTGTCTAGGC TCTGTGTCGG CTCTCTCTGC TATCATGGGC 
GTGACTTTGA CGGCTGCTAT CGGCGGTGCT GACATGCCCG TCGTCATCAC TGTGCTGAAC 
AGCTACTCAG GCTGGGCCCT GTGTGCGGAG GGCTTCCTGC TCAACAATAA CCTGCTGACC 
ATCGTGGGCG CACTTATTGG CTCCTCTGGT GCTATCCTGT CCTACATCAT GTGTGTGGCA 
ATGAATCGCT CCCTGGCTAA TGTGATTCTT GGAGGCTATG GTACCACTTC AACAGCTGGT 
GGGAAACCCA TGGAAATTTC TGGCACACAT ACAGAAATTA ACCTTGACAA TGCAATTGAC 
ATGATTCGAG AAGCTAATAG CATTATTATT ACTCCAGGCT ATGGTCTCTG TGCTGCCAAA 
GCACAATACC CTATCGCCGA TTTGGTGAAG ATGCTGTCCG AACAGGGCAA AAAAGTCAGG 
TTTGGAATCC ACCCAGTTGC AGGCCGGATG CCTGGTCAGC TTAATGTGCT GCTGGCTGAG 
GCGGGGGTGC CATATGATAT TGTGTTGGAA ATGGATGAGA TCAACCATGA TTTCCCAGAC 
ACTGATTTGG TCCTTGTAAT TGGAGCTAAT GACACTGTTA ATTCAGCTGC TCAAGAAGAC 
CCCAACTCTA TTATTGCAGG CATGCCAGTT CTCGAGGTCT GGAAATCAAA GCAGGTCATT 
GTTATGAAGA GGTCCTTGGG TGTTGGCTAT GCTGCAGTGG ACAATCCAAT CTTCTACAAA 
CCTAACACCG CCATGCTTCT AGGAGATGCA AAGAAGACAT GTGATGCACT GCAGGCAAAA 
GTTAGAGAAT CCTACCAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006912507.1
CDS	151..3414
Translation	MNMANLLKTVVTGCSCPLLSNLGSYKVLPGKKDVLRTFHTHRALCKSSVKPGIPYKQLTVGLPKEIFQNEKRVALSPAGVQILVKQGFNVAVESGAGEASKFSDDHYRAAGAQIQGTKEVLASDLVVKVRAPMINPVLNSHEADLLKTSGTLISFIYPAQNPDLLNKLSKRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPVLFSKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGEVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTTASAYTAGEDPInullGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLALMGGHLYPSTTSEGLAALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLSALYSGYNIEQIMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVSGGLAATLGGLNPSPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFAMDPAANLTKIVAYLGTYIGGVTFSGSLIAYGKLQGILKSAPLLLPGRHALNAGLLAASVGGMIPFMMDPSFTTGIICLGSVSALSAIMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Target ORF information:

RefSeq Version	XM_006912445.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto nicotinamide nucleotide transhydrogenase (NNT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006912445.2

ORF Insert Sequence:

ATGAACATGG CGAACCTACT GAAAACAGTG GTGACTGGCT GTTCGTGTCC TTTACTTAGC 
AATTTGGGGT CCTATAAGGT TCTACCTGGG AAGAAGGATG TTTTACGAAC ATTTCATACT 
CATCGGGCAC TGTGTAAATC CTCTGTAAAA CCAGGAATTC CATATAAACA ATTGACGGTT 
GGACTCCCCA AGGAGATTTT CCAAAATGAG AAGCGAGTGG CTTTGTCTCC TGCTGGCGTT 
CAGATCCTGG TCAAGCAGGG TTTTAATGTT GCTGTGGAAT CGGGTGCGGG AGAAGCTTCT 
AAGTTCTCAG ATGATCACTA CAGAGCGGCA GGTGCCCAAA TCCAAGGGAC GAAGGAAGTG 
CTGGCTTCCG ATTTGGTGGT CAAAGTGCGA GCCCCCATGA TTAATCCAGT ATTAAATAGT 
CATGAAGCGG ACCTTTTAAA GACATCGGGA ACACTTATTA GTTTTATTTA TCCAGCCCAA 
AATCCAGACT TGCTAAATAA ACTTTCCAAA AGAAAAACTA CAGTTTTGGC AATGGACCAG 
GTTCCAAGAG TCACAATTGC TCAGGGATAT GATGCGCTAA GCTCCATGGC CAACATTGCT 
GGGTATAAGG CTGTTGTACT AGCAGCAAAT CATTTTGGAC GATTTTTTAC TGGTCAGATC 
ACAGCTGCTG GAAAGGTTCC TCCAGCTAAG ATTCTGATAG TTGGCGGTGG AGTTGCTGGG 
CTTGCTTCTG CAGGCGCAGC CAAGTCAATG GGTGCAATTG TTCGAGGATT TGACACGAGA 
GCTGCAGCTT TGGAGCAGTT CAAATCTCTT GGTGCTGAGC CCTTAGAGGT AGACTTGAAG 
GAATCTGGTG AGGGACAAGG CGGATATGCG AAAGAGATGT CTAAAGAGTT CATTGAAGCT 
GAAATGAAGC TCTTTGCTCA GCAGTGCAAG GAGGTGGACA TCCTTATCAG CACAGCACTT 
ATTCCAGGTA AAAAAGCTCC AGTTTTATTT AGTAAAGAAA TGATCGAGTC AATGAAGGAA 
GGCTCAGTTG TTGTGGATTT AGCTGCTGAG GCTGGCGGAA ACTTTGAAAC CACTAAGCCA 
GGAGAACTGT ATGTTCATAA GGGAATTACT CACATAGGCT ATACGGACCT TCCCAGCCGA 
ATGGCTACTC AAGCCAGCAC CCTGTATTCC AATAACATCA CCAAGCTCCT GAAGGCCATC 
AGCCCTGACA AAGATAATTT TTATTTTGAA GTGAAAGATG ACTTTGACTT TGGTACAATG 
GGTCATGTCA TTAGAGGAAC TGTAGTGATG AAGGATGGTG AAGTGATTTT CCCTGCTCCC 
ACACCTAAAA ATATTCCTCA AGGTGCCCCA GTAAAACAGA AGACAGTGGC TGAGCTGGAA 
GCTGAAAAAG CGGCTACTAT TACACCCTTC AGGAAAACGA TGACCACGGC TTCTGCATAT 
ACAGCAGGTG AGGATCCCAT TNNNGGTTTG GGCATTGCAG CTCCCAATTT AGCATTTTCC 
CAGATGGTAA CCACCTTTGG CTTGGCCGGC ATTGTGGGCT ACCACACAGT CTGGGGAGTG 
ACTCCTGCCC TCCACTCACC ACTGATGTCT GTGACTAATG CAATCTCAGG GTTGACTGCA 
GTTGGTGGGT TGGCACTGAT GGGAGGACAT TTGTATCCTT CCACAACTTC TGAGGGCCTT 
GCTGCTCTTG CTACATTCAT ATCCTCAGTC AACATTGCAG GTGGCTTCCT GGTGACTCAG 
AGAATGCTGG ACATGTTTAA GCGTCCCACC GATCCCCCAG AGTACAATTA TCTGTATCTG 
CTCCCTGCTG GTACCTTTGT TGGAGGATAT TTATCTGCCC TCTACAGTGG TTATAACATT 
GAGCAAATCA TGTACCTAGG CTCAGGACTG TGCTGTGTTG GTGCCCTGGC TGGCCTTTCC 
ACCCAGGGAA CAGCTCGTCT TGGCAATGCA CTGGGAATGA TTGGGGTGTC TGGAGGCCTG 
GCAGCCACCC TTGGAGGCTT AAACCCGAGC CCAGAATTGT TAGCTCAGAT GTCTGGAGCC 
ATGGCTTTGG GCGGTACCAT TGGATTGACA ATTGCCAAGC GCATCCAGAT TTCTGATTTA 
CCTCAGTTAG TTGCTGCTTT CCACAGTTTA GTAGGCTTGG CAGCTGTGCT TACTTGCATA 
GCTGAGTATA TTATAGAATA TCCACATTTT GCTATGGATC CAGCAGCAAA TCTCACCAAG 
ATCGTGGCCT ACCTCGGTAC TTACATCGGT GGTGTCACAT TTAGTGGGTC TCTTATTGCC 
TATGGAAAAT TGCAGGGTAT TCTGAAATCT GCCCCTCTCC TGCTCCCCGG AAGGCATGCA 
CTCAATGCAG GCCTGCTGGC TGCTAGTGTG GGTGGAATGA TCCCATTCAT GATGGACCCC 
AGCTTTACCA CGGGCATTAT CTGTCTAGGC TCTGTGTCGG CTCTCTCTGC TATCATGGGC 
GTGACTTTGA CGGCTGCTAT CGGCGGTGCT GACATGCCCG TCGTCATCAC TGTGCTGAAC 
AGCTACTCAG GCTGGGCCCT GTGTGCGGAG GGCTTCCTGC TCAACAATAA CCTGCTGACC 
ATCGTGGGCG CACTTATTGG CTCCTCTGGT GCTATCCTGT CCTACATCAT GTGTGTGGCA 
ATGAATCGCT CCCTGGCTAA TGTGATTCTT GGAGGCTATG GTACCACTTC AACAGCTGGT 
GGGAAACCCA TGGAAATTTC TGGCACACAT ACAGAAATTA ACCTTGACAA TGCAATTGAC 
ATGATTCGAG AAGCTAATAG CATTATTATT ACTCCAGGCT ATGGTCTCTG TGCTGCCAAA 
GCACAATACC CTATCGCCGA TTTGGTGAAG ATGCTGTCCG AACAGGGCAA AAAAGTCAGG 
TTTGGAATCC ACCCAGTTGC AGGCCGGATG CCTGGTCAGC TTAATGTGCT GCTGGCTGAG 
GCGGGGGTGC CATATGATAT TGTGTTGGAA ATGGATGAGA TCAACCATGA TTTCCCAGAC 
ACTGATTTGG TCCTTGTAAT TGGAGCTAAT GACACTGTTA ATTCAGCTGC TCAAGAAGAC 
CCCAACTCTA TTATTGCAGG CATGCCAGTT CTCGAGGTCT GGAAATCAAA GCAGGTCATT 
GTTATGAAGA GGTCCTTGGG TGTTGGCTAT GCTGCAGTGG ACAATCCAAT CTTCTACAAA 
CCTAACACCG CCATGCTTCT AGGAGATGCA AAGAAGACAT GTGATGCACT GCAGGCAAAA 
GTTAGAGAAT CCTACCAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NNT ( XM_025047215.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_024902983.1 Pteropus alecto nicotinamide nucleotide transhydrogenase (NNT), mRNA.

Price & Availability↑

CloneID	OPt15298
Clone ID Related Accession (Same CDS sequence)	XM_006912445.2 , XM_025047215.1
Accession Version	XM_025047215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	NAD(P) transhydrogenase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006436284.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGG CGAACCTACT GAAAACAGTG GTGACTGGCT GTTCGTGTCC TTTACTTAGC 
AATTTGGGGT CCTATAAGGT TCTACCTGGG AAGAAGGATG TTTTACGAAC ATTTCATACT 
CATCGGGCAC TGTGTAAATC CTCTGTAAAA CCAGGAATTC CATATAAACA ATTGACGGTT 
GGACTCCCCA AGGAGATTTT CCAAAATGAG AAGCGAGTGG CTTTGTCTCC TGCTGGCGTT 
CAGATCCTGG TCAAGCAGGG TTTTAATGTT GCTGTGGAAT CGGGTGCGGG AGAAGCTTCT 
AAGTTCTCAG ATGATCACTA CAGAGCGGCA GGTGCCCAAA TCCAAGGGAC GAAGGAAGTG 
CTGGCTTCCG ATTTGGTGGT CAAAGTGCGA GCCCCCATGA TTAATCCAGT ATTAAATAGT 
CATGAAGCGG ACCTTTTAAA GACATCGGGA ACACTTATTA GTTTTATTTA TCCAGCCCAA 
AATCCAGACT TGCTAAATAA ACTTTCCAAA AGAAAAACTA CAGTTTTGGC AATGGACCAG 
GTTCCAAGAG TCACAATTGC TCAGGGATAT GATGCGCTAA GCTCCATGGC CAACATTGCT 
GGGTATAAGG CTGTTGTACT AGCAGCAAAT CATTTTGGAC GATTTTTTAC TGGTCAGATC 
ACAGCTGCTG GAAAGGTTCC TCCAGCTAAG ATTCTGATAG TTGGCGGTGG AGTTGCTGGG 
CTTGCTTCTG CAGGCGCAGC CAAGTCAATG GGTGCAATTG TTCGAGGATT TGACACGAGA 
GCTGCAGCTT TGGAGCAGTT CAAATCTCTT GGTGCTGAGC CCTTAGAGGT AGACTTGAAG 
GAATCTGGTG AGGGACAAGG CGGATATGCG AAAGAGATGT CTAAAGAGTT CATTGAAGCT 
GAAATGAAGC TCTTTGCTCA GCAGTGCAAG GAGGTGGACA TCCTTATCAG CACAGCACTT 
ATTCCAGGTA AAAAAGCTCC AGTTTTATTT AGTAAAGAAA TGATCGAGTC AATGAAGGAA 
GGCTCAGTTG TTGTGGATTT AGCTGCTGAG GCTGGCGGAA ACTTTGAAAC CACTAAGCCA 
GGAGAACTGT ATGTTCATAA GGGAATTACT CACATAGGCT ATACGGACCT TCCCAGCCGA 
ATGGCTACTC AAGCCAGCAC CCTGTATTCC AATAACATCA CCAAGCTCCT GAAGGCCATC 
AGCCCTGACA AAGATAATTT TTATTTTGAA GTGAAAGATG ACTTTGACTT TGGTACAATG 
GGTCATGTCA TTAGAGGAAC TGTAGTGATG AAGGATGGTG AAGTGATTTT CCCTGCTCCC 
ACACCTAAAA ATATTCCTCA AGGTGCCCCA GTAAAACAGA AGACAGTGGC TGAGCTGGAA 
GCTGAAAAAG CGGCTACTAT TACACCCTTC AGGAAAACGA TGACCACGGC TTCTGCATAT 
ACAGCAGGTG AGGATCCCAT TNNNGGTTTG GGCATTGCAG CTCCCAATTT AGCATTTTCC 
CAGATGGTAA CCACCTTTGG CTTGGCCGGC ATTGTGGGCT ACCACACAGT CTGGGGAGTG 
ACTCCTGCCC TCCACTCACC ACTGATGTCT GTGACTAATG CAATCTCAGG GTTGACTGCA 
GTTGGTGGGT TGGCACTGAT GGGAGGACAT TTGTATCCTT CCACAACTTC TGAGGGCCTT 
GCTGCTCTTG CTACATTCAT ATCCTCAGTC AACATTGCAG GTGGCTTCCT GGTGACTCAG 
AGAATGCTGG ACATGTTTAA GCGTCCCACC GATCCCCCAG AGTACAATTA TCTGTATCTG 
CTCCCTGCTG GTACCTTTGT TGGAGGATAT TTATCTGCCC TCTACAGTGG TTATAACATT 
GAGCAAATCA TGTACCTAGG CTCAGGACTG TGCTGTGTTG GTGCCCTGGC TGGCCTTTCC 
ACCCAGGGAA CAGCTCGTCT TGGCAATGCA CTGGGAATGA TTGGGGTGTC TGGAGGCCTG 
GCAGCCACCC TTGGAGGCTT AAACCCGAGC CCAGAATTGT TAGCTCAGAT GTCTGGAGCC 
ATGGCTTTGG GCGGTACCAT TGGATTGACA ATTGCCAAGC GCATCCAGAT TTCTGATTTA 
CCTCAGTTAG TTGCTGCTTT CCACAGTTTA GTAGGCTTGG CAGCTGTGCT TACTTGCATA 
GCTGAGTATA TTATAGAATA TCCACATTTT GCTATGGATC CAGCAGCAAA TCTCACCAAG 
ATCGTGGCCT ACCTCGGTAC TTACATCGGT GGTGTCACAT TTAGTGGGTC TCTTATTGCC 
TATGGAAAAT TGCAGGGTAT TCTGAAATCT GCCCCTCTCC TGCTCCCCGG AAGGCATGCA 
CTCAATGCAG GCCTGCTGGC TGCTAGTGTG GGTGGAATGA TCCCATTCAT GATGGACCCC 
AGCTTTACCA CGGGCATTAT CTGTCTAGGC TCTGTGTCGG CTCTCTCTGC TATCATGGGC 
GTGACTTTGA CGGCTGCTAT CGGCGGTGCT GACATGCCCG TCGTCATCAC TGTGCTGAAC 
AGCTACTCAG GCTGGGCCCT GTGTGCGGAG GGCTTCCTGC TCAACAATAA CCTGCTGACC 
ATCGTGGGCG CACTTATTGG CTCCTCTGGT GCTATCCTGT CCTACATCAT GTGTGTGGCA 
ATGAATCGCT CCCTGGCTAA TGTGATTCTT GGAGGCTATG GTACCACTTC AACAGCTGGT 
GGGAAACCCA TGGAAATTTC TGGCACACAT ACAGAAATTA ACCTTGACAA TGCAATTGAC 
ATGATTCGAG AAGCTAATAG CATTATTATT ACTCCAGGCT ATGGTCTCTG TGCTGCCAAA 
GCACAATACC CTATCGCCGA TTTGGTGAAG ATGCTGTCCG AACAGGGCAA AAAAGTCAGG 
TTTGGAATCC ACCCAGTTGC AGGCCGGATG CCTGGTCAGC TTAATGTGCT GCTGGCTGAG 
GCGGGGGTGC CATATGATAT TGTGTTGGAA ATGGATGAGA TCAACCATGA TTTCCCAGAC 
ACTGATTTGG TCCTTGTAAT TGGAGCTAAT GACACTGTTA ATTCAGCTGC TCAAGAAGAC 
CCCAACTCTA TTATTGCAGG CATGCCAGTT CTCGAGGTCT GGAAATCAAA GCAGGTCATT 
GTTATGAAGA GGTCCTTGGG TGTTGGCTAT GCTGCAGTGG ACAATCCAAT CTTCTACAAA 
CCTAACACCG CCATGCTTCT AGGAGATGCA AAGAAGACAT GTGATGCACT GCAGGCAAAA 
GTTAGAGAAT CCTACCAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024902983.1
CDS	153..3416
Translation	MNMANLLKTVVTGCSCPLLSNLGSYKVLPGKKDVLRTFHTHRALCKSSVKPGIPYKQLTVGLPKEIFQNEKRVALSPAGVQILVKQGFNVAVESGAGEASKFSDDHYRAAGAQIQGTKEVLASDLVVKVRAPMINPVLNSHEADLLKTSGTLISFIYPAQNPDLLNKLSKRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPVLFSKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGEVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTTASAYTAGEDPInullGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLALMGGHLYPSTTSEGLAALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLSALYSGYNIEQIMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVSGGLAATLGGLNPSPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFAMDPAANLTKIVAYLGTYIGGVTFSGSLIAYGKLQGILKSAPLLLPGRHALNAGLLAASVGGMIPFMMDPSFTTGIICLGSVSALSAIMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Target ORF information:

RefSeq Version	XM_025047215.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto nicotinamide nucleotide transhydrogenase (NNT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025047215.1

ORF Insert Sequence:

ATGAACATGG CGAACCTACT GAAAACAGTG GTGACTGGCT GTTCGTGTCC TTTACTTAGC 
AATTTGGGGT CCTATAAGGT TCTACCTGGG AAGAAGGATG TTTTACGAAC ATTTCATACT 
CATCGGGCAC TGTGTAAATC CTCTGTAAAA CCAGGAATTC CATATAAACA ATTGACGGTT 
GGACTCCCCA AGGAGATTTT CCAAAATGAG AAGCGAGTGG CTTTGTCTCC TGCTGGCGTT 
CAGATCCTGG TCAAGCAGGG TTTTAATGTT GCTGTGGAAT CGGGTGCGGG AGAAGCTTCT 
AAGTTCTCAG ATGATCACTA CAGAGCGGCA GGTGCCCAAA TCCAAGGGAC GAAGGAAGTG 
CTGGCTTCCG ATTTGGTGGT CAAAGTGCGA GCCCCCATGA TTAATCCAGT ATTAAATAGT 
CATGAAGCGG ACCTTTTAAA GACATCGGGA ACACTTATTA GTTTTATTTA TCCAGCCCAA 
AATCCAGACT TGCTAAATAA ACTTTCCAAA AGAAAAACTA CAGTTTTGGC AATGGACCAG 
GTTCCAAGAG TCACAATTGC TCAGGGATAT GATGCGCTAA GCTCCATGGC CAACATTGCT 
GGGTATAAGG CTGTTGTACT AGCAGCAAAT CATTTTGGAC GATTTTTTAC TGGTCAGATC 
ACAGCTGCTG GAAAGGTTCC TCCAGCTAAG ATTCTGATAG TTGGCGGTGG AGTTGCTGGG 
CTTGCTTCTG CAGGCGCAGC CAAGTCAATG GGTGCAATTG TTCGAGGATT TGACACGAGA 
GCTGCAGCTT TGGAGCAGTT CAAATCTCTT GGTGCTGAGC CCTTAGAGGT AGACTTGAAG 
GAATCTGGTG AGGGACAAGG CGGATATGCG AAAGAGATGT CTAAAGAGTT CATTGAAGCT 
GAAATGAAGC TCTTTGCTCA GCAGTGCAAG GAGGTGGACA TCCTTATCAG CACAGCACTT 
ATTCCAGGTA AAAAAGCTCC AGTTTTATTT AGTAAAGAAA TGATCGAGTC AATGAAGGAA 
GGCTCAGTTG TTGTGGATTT AGCTGCTGAG GCTGGCGGAA ACTTTGAAAC CACTAAGCCA 
GGAGAACTGT ATGTTCATAA GGGAATTACT CACATAGGCT ATACGGACCT TCCCAGCCGA 
ATGGCTACTC AAGCCAGCAC CCTGTATTCC AATAACATCA CCAAGCTCCT GAAGGCCATC 
AGCCCTGACA AAGATAATTT TTATTTTGAA GTGAAAGATG ACTTTGACTT TGGTACAATG 
GGTCATGTCA TTAGAGGAAC TGTAGTGATG AAGGATGGTG AAGTGATTTT CCCTGCTCCC 
ACACCTAAAA ATATTCCTCA AGGTGCCCCA GTAAAACAGA AGACAGTGGC TGAGCTGGAA 
GCTGAAAAAG CGGCTACTAT TACACCCTTC AGGAAAACGA TGACCACGGC TTCTGCATAT 
ACAGCAGGTG AGGATCCCAT TNNNGGTTTG GGCATTGCAG CTCCCAATTT AGCATTTTCC 
CAGATGGTAA CCACCTTTGG CTTGGCCGGC ATTGTGGGCT ACCACACAGT CTGGGGAGTG 
ACTCCTGCCC TCCACTCACC ACTGATGTCT GTGACTAATG CAATCTCAGG GTTGACTGCA 
GTTGGTGGGT TGGCACTGAT GGGAGGACAT TTGTATCCTT CCACAACTTC TGAGGGCCTT 
GCTGCTCTTG CTACATTCAT ATCCTCAGTC AACATTGCAG GTGGCTTCCT GGTGACTCAG 
AGAATGCTGG ACATGTTTAA GCGTCCCACC GATCCCCCAG AGTACAATTA TCTGTATCTG 
CTCCCTGCTG GTACCTTTGT TGGAGGATAT TTATCTGCCC TCTACAGTGG TTATAACATT 
GAGCAAATCA TGTACCTAGG CTCAGGACTG TGCTGTGTTG GTGCCCTGGC TGGCCTTTCC 
ACCCAGGGAA CAGCTCGTCT TGGCAATGCA CTGGGAATGA TTGGGGTGTC TGGAGGCCTG 
GCAGCCACCC TTGGAGGCTT AAACCCGAGC CCAGAATTGT TAGCTCAGAT GTCTGGAGCC 
ATGGCTTTGG GCGGTACCAT TGGATTGACA ATTGCCAAGC GCATCCAGAT TTCTGATTTA 
CCTCAGTTAG TTGCTGCTTT CCACAGTTTA GTAGGCTTGG CAGCTGTGCT TACTTGCATA 
GCTGAGTATA TTATAGAATA TCCACATTTT GCTATGGATC CAGCAGCAAA TCTCACCAAG 
ATCGTGGCCT ACCTCGGTAC TTACATCGGT GGTGTCACAT TTAGTGGGTC TCTTATTGCC 
TATGGAAAAT TGCAGGGTAT TCTGAAATCT GCCCCTCTCC TGCTCCCCGG AAGGCATGCA 
CTCAATGCAG GCCTGCTGGC TGCTAGTGTG GGTGGAATGA TCCCATTCAT GATGGACCCC 
AGCTTTACCA CGGGCATTAT CTGTCTAGGC TCTGTGTCGG CTCTCTCTGC TATCATGGGC 
GTGACTTTGA CGGCTGCTAT CGGCGGTGCT GACATGCCCG TCGTCATCAC TGTGCTGAAC 
AGCTACTCAG GCTGGGCCCT GTGTGCGGAG GGCTTCCTGC TCAACAATAA CCTGCTGACC 
ATCGTGGGCG CACTTATTGG CTCCTCTGGT GCTATCCTGT CCTACATCAT GTGTGTGGCA 
ATGAATCGCT CCCTGGCTAA TGTGATTCTT GGAGGCTATG GTACCACTTC AACAGCTGGT 
GGGAAACCCA TGGAAATTTC TGGCACACAT ACAGAAATTA ACCTTGACAA TGCAATTGAC 
ATGATTCGAG AAGCTAATAG CATTATTATT ACTCCAGGCT ATGGTCTCTG TGCTGCCAAA 
GCACAATACC CTATCGCCGA TTTGGTGAAG ATGCTGTCCG AACAGGGCAA AAAAGTCAGG 
TTTGGAATCC ACCCAGTTGC AGGCCGGATG CCTGGTCAGC TTAATGTGCT GCTGGCTGAG 
GCGGGGGTGC CATATGATAT TGTGTTGGAA ATGGATGAGA TCAACCATGA TTTCCCAGAC 
ACTGATTTGG TCCTTGTAAT TGGAGCTAAT GACACTGTTA ATTCAGCTGC TCAAGAAGAC 
CCCAACTCTA TTATTGCAGG CATGCCAGTT CTCGAGGTCT GGAAATCAAA GCAGGTCATT 
GTTATGAAGA GGTCCTTGGG TGTTGGCTAT GCTGCAGTGG ACAATCCAAT CTTCTACAAA 
CCTAACACCG CCATGCTTCT AGGAGATGCA AAGAAGACAT GTGATGCACT GCAGGCAAAA 
GTTAGAGAAT CCTACCAGAA GTAA