RTN4 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	RTN4
Entrez Gene ID	102892913
Full Name	reticulon 4
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025045097.1	XP_024900865.1	reticulon-4
XM_015587493.1	XP_015442979.1	reticulon-4

Pathways from BioSystems
Gene Ontology

The following RTN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RTN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt85451	XM_025045097.1 Latest version!	Pteropus alecto reticulon 4 (RTN4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPt11705	XM_015587493.1 Latest version!	Pteropus alecto reticulon 4 (RTN4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RTN4 ( XM_025045097.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_024900865.1 Pteropus alecto reticulon 4 (RTN4), mRNA.

Price & Availability↑

CloneID	OPt85451
Clone ID Related Accession (Same CDS sequence)	XM_025045097.1
Accession Version	XM_025045097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	reticulon-4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006434709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA TGGAGCAGTC GCCTCTGGTC TCCTCGTCCT CGGAAAGCCC TCCCCGGCCT 
CAGCCCCCGT TCAAGTACCA GTTCGTGCAG GATTCCGAGG ACGAAGAGGA CGAGGAGCCC 
GAGGACGAGG AGGACGAGGA GCCCGAGGAA GAGGACGAGG ACCTGGAGGA GCTGGAGGTG 
CTGGAGAGGA AAGATTTAAC TGAGGTTTCA GAATTGGAAT ATTCAGAAAT GGGATCATCA 
TTCATTGTCT CTCCAAAAGC AGAATCTGCC GTAACAGTCG CAGACCTTAG GGAAGAAATA 
ATTGTGAAGA ATAAAGATGA AGAGGAGGAT TTAGTTAGCA ATAACATCCT TCGTAATCAA 
CAAGAGTCAC CTGTAACCCT TACTCAGTTG ATTAAAGAAG AGAACACAGT CATGTCTCCA 
GAAGAAACAA AGGACAGTTT TAAGGAAAAG GGACTCATAG CGGGAGCGCC TATGAGGGAA 
GAATATGCAG ACTTCAAACC ATTTGAACGA GTATGGGAAG TAAAAGATAT TTATAAGCAA 
GGTAGTGATG TGCTGGCTAC TGCAGGTAGT ACAGAGAGCA AGTTGGAAAG TAAAGTGGAT 
AAGAAATGTT TTGCAGATAG CACTGAGCAA ACAAATCATG AAAGAGATAG TGAAAGCAGT 
AATGATGATA TTTCTTTTCC CAGTACACCG GAAGCTATAA AAGTTGGTTC AGGGGCATAT 
ATCACATGCA CTCCCTTTAA CCCAAAAGCA ACTGAAGGCA TTTCAACAAA TGTTTTTCCT 
TTGTTGGAAG ATCATACTTC AGAAAATAAG ACAGATGAAA AAAAAATAGA AGAAAAAAAG 
GCCCAGATTG TAACAGAGAA GAATACTAGC ATTACATCTT CGAACCCTTT CCTTGTAGCA 
ACACAAGATT CTGAGACAGG TTATGTCAAA ACCGATAATT TATCAAAGAT GACTGAGAAA 
GTAGTGGCAA ACATGCCTGA AGGTCTAACC CCAGATTTAG TACAGGAAGC ATGTGGAAGT 
GAATTGAATG AAACTATAGG GACAAAGATT GCTTTTGAAA CAAAAATGGA CTTGGTTCAA 
ACATCAGAAG CTGTGCAGGA GTCACTCTAC CCTGTAGCAC AGCTTTGCCC ATCATTTGAA 
GAATCTGAAG CTACTCCTTC GCCAGTTTTG CCTGACATTG TTATGGAAGC ACCATTAAAT 
TCTGTAGTTC CTAGTGCTGG TGCTTCTGCA GTGCAGCCCA GTTCATCACC ATTAGAAGCT 
CCCCTTTCAG TTAATTATGA AAGCATGAAA CTTGAGCCTG AAAATCCACC ACCATATGAA 
GAGGCAATGA ATGTATCACT AAAAAAAGTA TCAGGAACAA AGGAAGAAAT TAAAGAGCCT 
GAGAGTATTA ATGTAGCTGT TCAAGAAACA GAAGCTCCTT ATATATCTAT TGCATGTGAT 
TTAATTAAAG AAACAAAGCT CTCTACTGAA CCAACTCCAG ATTTCTCTAA TTATTCAGAA 
ATAGCAAAAG TTGCACAGCC AGCGCCTGAT CATTCTGAGC TTGTTGAAGA TTCCTCACCT 
GATTCTGAAC CAGTTGACTT ATTTAGTGAT GATTCAATAC CTGAAGTTCC ACAAAAACAA 
GATAAAGCTA TGATATTTGT GAAAGAGAAT TTTGTTGAAA CTTCATCTGA GTTAATGACA 
GAACATGAAA ATAAGGAAAA ACTCGGTGCT TCACCACCAC CTAAGGCGGG GAAGCCATAT 
TTGGAATCTC TTCAGCCCAG TTCAGACACC ACAAAAGATA CCTTATCACC TGGTGAAGTT 
TCAGCATTGA CCCCAAGGGA GAAAATGCCT TTGCAGATGG AGGAGCTCAA TACTGCAATT 
TATTCAAGTG ATGGCTTACT TATTGCTAAG GAAGCAAAGA TAAGAGATAG TGAAACATTT 
TCAGATTCAT CTCCAATTGA GATTATAGAT GAGTTCCCTA CATTTGTCAG TTCCAAAACT 
GATTCTTCTA AATATACCAG GGAATACACT GACCTAGAAG TATCCCACAA AAGGGAAATT 
GCTAATGTCC AGGATGGAGC CGGCTCATTG CCTTGCTCTG AATTACCCCA AGACCTTATT 
TTCAAGAATG TGCAACATAA AGGTGAAGAT AAAGCCCATG TCCCAGATGA AATCTCTAAA 
GATAGATCTG ATATTTCAAA GATGCCCTTA CTGCCTCCAG ATGCTTCTGC TCTGGCTGCT 
CAAACAGAGA TAGGTAGTAT AGTTAAACCC AAAGTTCTTG TTAAAGAAGC TGAGAAAAAA 
CTTCCTTCTG GTACAGAAAA AGAGAACAGA TCACCGTCTG CTATATTTTC AGCAGAGCTG 
AGTAAAACTT CAGTGGTCGA CCTCCTCTAC TGGAGAGACA TTAAGAAGAC TGGAGTGGTG 
TTTGGTGCCA GCNNNNNNCT TCTCTTTTCA TTGACAGTAT TCAGCATTGT GAGTGTAACG 
GCCTACATCG CCTTGGCCCT GCTCTCTGTG ACTATCAGCT TTAGGATATA TAAGGGTGTG 
ATCCAAGCTA TCCAGAAATC AGATGAAGGC CACCCATTCA GGGCATATTT GGAATCTGAA 
GTTGCTATAT CTGAGGAATT GGTTCAGAAA TACAGTAATT CTGCTCTTGG TCATGTGAAC 
TGCACGATAA AAGAACTCAG GCGCCTCTTC TTAGTTGACG ATTTAGTTGA TTCTCTGAAG 
TTTGCAGTGT TGATGTGGGT ATTTACCTAT GTTGGTGCCT TGTTCAATGG TCTGACACTA 
CTGATTTTGG CTCTGATTTC ACTCTTCAGC GTTCCTGTTA TTTATGAACG GCATCAGGCG 
CAGATAGATC ATTACCTAGG ACTTGCAAAT AAGAATGTTA AAGATGCTAT GGCTAAAATC 
CAAGCAAAAA TCCCTGGATT GAAACGCAAA GCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024900865.1
CDS	44..2962
Translation	MEDMEQSPLVSSSSESPPRPQPPFKYQFVQDSEDEEDEEPEDEEDEEPEEEDEDLEELEVLERKDLTEVSELEYSEMGSSFIVSPKAESAVTVADLREEIIVKNKDEEEDLVSNNILRNQQESPVTLTQLIKEENTVMSPEETKDSFKEKGLIAGAPMREEYADFKPFERVWEVKDIYKQGSDVLATAGSTESKLESKVDKKCFADSTEQTNHERDSESSNDDISFPSTPEAIKVGSGAYITCTPFNPKATEGISTNVFPLLEDHTSENKTDEKKIEEKKAQIVTEKNTSITSSNPFLVATQDSETGYVKTDNLSKMTEKVVANMPEGLTPDLVQEACGSELNETIGTKIAFETKMDLVQTSEAVQESLYPVAQLCPSFEESEATPSPVLPDIVMEAPLNSVVPSAGASAVQPSSSPLEAPLSVNYESMKLEPENPPPYEEAMNVSLKKVSGTKEEIKEPESINVAVQETEAPYISIACDLIKETKLSTEPTPDFSNYSEIAKVAQPAPDHSELVEDSSPDSEPVDLFSDDSIPEVPQKQDKAMIFVKENFVETSSELMTEHENKEKLGASPPPKAGKPYLESLQPSSDTTKDTLSPGEVSALTPREKMPLQMEELNTAIYSSDGLLIAKEAKIRDSETFSDSSPIEIIDEFPTFVSSKTDSSKYTREYTDLEVSHKREIANVQDGAGSLPCSELPQDLIFKNVQHKGEDKAHVPDEISKDRSDISKMPLLPPDASALAAQTEIGSIVKPKVLVKEAEKKLPSGTEKENRSPSAIFSAELSKTSVVDLLYWRDIKKTGVVFGASnullnullLLFSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNCTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFSVPVIYERHQAQIDHYLGLANKNVKDAMAKIQAKIPGLKRKAE

Target ORF information:

RefSeq Version	XM_025045097.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto reticulon 4 (RTN4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025045097.1

ORF Insert Sequence:

ATGGAAGACA TGGAGCAGTC GCCTCTGGTC TCCTCGTCCT CGGAAAGCCC TCCCCGGCCT 
CAGCCCCCGT TCAAGTACCA GTTCGTGCAG GATTCCGAGG ACGAAGAGGA CGAGGAGCCC 
GAGGACGAGG AGGACGAGGA GCCCGAGGAA GAGGACGAGG ACCTGGAGGA GCTGGAGGTG 
CTGGAGAGGA AAGATTTAAC TGAGGTTTCA GAATTGGAAT ATTCAGAAAT GGGATCATCA 
TTCATTGTCT CTCCAAAAGC AGAATCTGCC GTAACAGTCG CAGACCTTAG GGAAGAAATA 
ATTGTGAAGA ATAAAGATGA AGAGGAGGAT TTAGTTAGCA ATAACATCCT TCGTAATCAA 
CAAGAGTCAC CTGTAACCCT TACTCAGTTG ATTAAAGAAG AGAACACAGT CATGTCTCCA 
GAAGAAACAA AGGACAGTTT TAAGGAAAAG GGACTCATAG CGGGAGCGCC TATGAGGGAA 
GAATATGCAG ACTTCAAACC ATTTGAACGA GTATGGGAAG TAAAAGATAT TTATAAGCAA 
GGTAGTGATG TGCTGGCTAC TGCAGGTAGT ACAGAGAGCA AGTTGGAAAG TAAAGTGGAT 
AAGAAATGTT TTGCAGATAG CACTGAGCAA ACAAATCATG AAAGAGATAG TGAAAGCAGT 
AATGATGATA TTTCTTTTCC CAGTACACCG GAAGCTATAA AAGTTGGTTC AGGGGCATAT 
ATCACATGCA CTCCCTTTAA CCCAAAAGCA ACTGAAGGCA TTTCAACAAA TGTTTTTCCT 
TTGTTGGAAG ATCATACTTC AGAAAATAAG ACAGATGAAA AAAAAATAGA AGAAAAAAAG 
GCCCAGATTG TAACAGAGAA GAATACTAGC ATTACATCTT CGAACCCTTT CCTTGTAGCA 
ACACAAGATT CTGAGACAGG TTATGTCAAA ACCGATAATT TATCAAAGAT GACTGAGAAA 
GTAGTGGCAA ACATGCCTGA AGGTCTAACC CCAGATTTAG TACAGGAAGC ATGTGGAAGT 
GAATTGAATG AAACTATAGG GACAAAGATT GCTTTTGAAA CAAAAATGGA CTTGGTTCAA 
ACATCAGAAG CTGTGCAGGA GTCACTCTAC CCTGTAGCAC AGCTTTGCCC ATCATTTGAA 
GAATCTGAAG CTACTCCTTC GCCAGTTTTG CCTGACATTG TTATGGAAGC ACCATTAAAT 
TCTGTAGTTC CTAGTGCTGG TGCTTCTGCA GTGCAGCCCA GTTCATCACC ATTAGAAGCT 
CCCCTTTCAG TTAATTATGA AAGCATGAAA CTTGAGCCTG AAAATCCACC ACCATATGAA 
GAGGCAATGA ATGTATCACT AAAAAAAGTA TCAGGAACAA AGGAAGAAAT TAAAGAGCCT 
GAGAGTATTA ATGTAGCTGT TCAAGAAACA GAAGCTCCTT ATATATCTAT TGCATGTGAT 
TTAATTAAAG AAACAAAGCT CTCTACTGAA CCAACTCCAG ATTTCTCTAA TTATTCAGAA 
ATAGCAAAAG TTGCACAGCC AGCGCCTGAT CATTCTGAGC TTGTTGAAGA TTCCTCACCT 
GATTCTGAAC CAGTTGACTT ATTTAGTGAT GATTCAATAC CTGAAGTTCC ACAAAAACAA 
GATAAAGCTA TGATATTTGT GAAAGAGAAT TTTGTTGAAA CTTCATCTGA GTTAATGACA 
GAACATGAAA ATAAGGAAAA ACTCGGTGCT TCACCACCAC CTAAGGCGGG GAAGCCATAT 
TTGGAATCTC TTCAGCCCAG TTCAGACACC ACAAAAGATA CCTTATCACC TGGTGAAGTT 
TCAGCATTGA CCCCAAGGGA GAAAATGCCT TTGCAGATGG AGGAGCTCAA TACTGCAATT 
TATTCAAGTG ATGGCTTACT TATTGCTAAG GAAGCAAAGA TAAGAGATAG TGAAACATTT 
TCAGATTCAT CTCCAATTGA GATTATAGAT GAGTTCCCTA CATTTGTCAG TTCCAAAACT 
GATTCTTCTA AATATACCAG GGAATACACT GACCTAGAAG TATCCCACAA AAGGGAAATT 
GCTAATGTCC AGGATGGAGC CGGCTCATTG CCTTGCTCTG AATTACCCCA AGACCTTATT 
TTCAAGAATG TGCAACATAA AGGTGAAGAT AAAGCCCATG TCCCAGATGA AATCTCTAAA 
GATAGATCTG ATATTTCAAA GATGCCCTTA CTGCCTCCAG ATGCTTCTGC TCTGGCTGCT 
CAAACAGAGA TAGGTAGTAT AGTTAAACCC AAAGTTCTTG TTAAAGAAGC TGAGAAAAAA 
CTTCCTTCTG GTACAGAAAA AGAGAACAGA TCACCGTCTG CTATATTTTC AGCAGAGCTG 
AGTAAAACTT CAGTGGTCGA CCTCCTCTAC TGGAGAGACA TTAAGAAGAC TGGAGTGGTG 
TTTGGTGCCA GCNNNNNNCT TCTCTTTTCA TTGACAGTAT TCAGCATTGT GAGTGTAACG 
GCCTACATCG CCTTGGCCCT GCTCTCTGTG ACTATCAGCT TTAGGATATA TAAGGGTGTG 
ATCCAAGCTA TCCAGAAATC AGATGAAGGC CACCCATTCA GGGCATATTT GGAATCTGAA 
GTTGCTATAT CTGAGGAATT GGTTCAGAAA TACAGTAATT CTGCTCTTGG TCATGTGAAC 
TGCACGATAA AAGAACTCAG GCGCCTCTTC TTAGTTGACG ATTTAGTTGA TTCTCTGAAG 
TTTGCAGTGT TGATGTGGGT ATTTACCTAT GTTGGTGCCT TGTTCAATGG TCTGACACTA 
CTGATTTTGG CTCTGATTTC ACTCTTCAGC GTTCCTGTTA TTTATGAACG GCATCAGGCG 
CAGATAGATC ATTACCTAGG ACTTGCAAAT AAGAATGTTA AAGATGCTAT GGCTAAAATC 
CAAGCAAAAA TCCCTGGATT GAAACGCAAA GCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTN4 ( XM_015587493.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015442979.1 Pteropus alecto reticulon 4 (RTN4), mRNA.

Price & Availability↑

CloneID	OPt11705
Clone ID Related Accession (Same CDS sequence)	XM_015587493.1
Accession Version	XM_015587493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	reticulon-4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006434709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA TGGAGCAGTC GCCTCTGGTC TCCTCGTCCT CGGAAAGCCC TCCCCGGCCT 
CAGCCCCCGT TCAAGTACCA GTTCGTGCAG GATTCCGAGG ACGAAGAGGA CGAGGAGCCC 
GAGGACGAGG AGGACGAGGA GCCCGAGGAA GAGGACGAGG ACCTGGAGGA GCTGGAGCCT 
CCTGCCCGGC CTCCCCCGCC TCCTCCGGCC GGCGTGAGCT CCCTGGCCGA GCCCGCGTGG 
ACCCCGGCCG CCGCCGGCCC CGCCGCGCCC CCCTCCACCC CGGCCGCGCC CAAGCGCAGG 
AGCTCCTCAG GCTCTGTGGA TGAGACACTT TTTGCTCTTC CTGCTGCATC TGAGCCTGTG 
ATACACTCCT CTGCAGAAAA AATTACGGAC TTGAAGGAGC AGCCAGGTAG CACTGTTTCC 
ACTGGTCAAG AGGATTTCCC ATCTGTCCTG CTTGAAACTG CTGCTTCTCT TCCTTCTCTA 
TCTCCCCTCT CAGCTGCTTC TTTTAAAGAA CATGAATACC TTGGTAATTT ACCAGCAGTA 
TTACCCACCA AAGGAACACT TCAAGAAACT TCAGATGAAG CTTCTAAAGA GTTTTCAGAG 
CAGGCAAAAA ATCCATTTGT AGACAGAGAT TTAACTGAGG TTTCAGAATT GGAATATTCA 
GAAATGGGAT CATCATTCAT TGTCTCTCCA AAAGCAGAAT CTGCCGTAAC AGTCGCAGAC 
CTTAGGGAAG AAATAATTGT GAAGAATAAA GATGAAGAGG AGGATTTAGT TAGCAATAAC 
ATCCTTCGTA ATCAACAAGA GTCACCTGTA ACCCTTACTC AGTTGATTAA AGAAGAGAAC 
ACAGTCATGT CTCCAGAAGA AACAAAGGAC AGTTTTAAGG AAAAGGGACT CATAGCGGGA 
GCGCCTATGA GGGAAGAATA TGCAGACTTC AAACCATTTG AACGAGTATG GGAAGTAAAA 
GATATTTATA AGCAAGGTAG TGATGTGCTG GCTACTGCAG GTAGTACAGA GAGCAAGTTG 
GAAAGTAAAG TGGATAAGAA ATGTTTTGCA GATAGCACTG AGCAAACAAA TCATGAAAGA 
GATAGTGAAA GCAGTAATGA TGATATTTCT TTTCCCAGTA CACCGGAAGC TATAAAAGTT 
GGTTCAGGGG CATATATCAC ATGCACTCCC TTTAACCCAA AAGCAACTGA AGGCATTTCA 
ACAAATGTTT TTCCTTTGTT GGAAGATCAT ACTTCAGAAA ATAAGACAGA TGAAAAAAAA 
ATAGAAGAAA AAAAGGCCCA GATTGTAACA GAGAAGAATA CTAGCATTAC ATCTTCGAAC 
CCTTTCCTTG TAGCAACACA AGATTCTGAG ACAGGTTATG TCAAAACCGA TAATTTATCA 
AAGATGACTG AGAAAGTAGT GGCAAACATG CCTGAAGGTC TAACCCCAGA TTTAGTACAG 
GAAGCATGTG GAAGTGAATT GAATGAAACT ATAGGGACAA AGATTGCTTT TGAAACAAAA 
ATGGACTTGG TTCAAACATC AGAAGCTGTG CAGGAGTCAC TCTACCCTGT AGCACAGCTT 
TGCCCATCAT TTGAAGAATC TGAAGCTACT CCTTCGCCAG TTTTGCCTGA CATTGTTATG 
GAAGCACCAT TAAATTCTGT AGTTCCTAGT GCTGGTGCTT CTGCAGTGCA GCCCAGTTCA 
TCACCATTAG AAGCTCCCCT TTCAGTTAAT TATGAAAGCA TGAAACTTGA GCCTGAAAAT 
CCACCACCAT ATGAAGAGGC AATGAATGTA TCACTAAAAA AAGTATCAGG AACAAAGGAA 
GAAATTAAAG AGCCTGAGAG TATTAATGTA GCTGTTCAAG AAACAGAAGC TCCTTATATA 
TCTATTGCAT GTGATTTAAT TAAAGAAACA AAGCTCTCTA CTGAACCAAC TCCAGATTTC 
TCTAATTATT CAGAAATAGC AAAAGTTGCA CAGCCAGCGC CTGATCATTC TGAGCTTGTT 
GAAGATTCCT CACCTGATTC TGAACCAGTT GACTTATTTA GTGATGATTC AATACCTGAA 
GTTCCACAAA AACAAGATAA AGCTATGATA TTTGTGAAAG AGAATTTTGT TGAAACTTCA 
TCTGAGTTAA TGACAGAACA TGAAAATAAG GAAAAACTCG GTGCTTCACC ACCACCTAAG 
GCGGGGAAGC CATATTTGGA ATCTCTTCAG CCCAGTTCAG ACACCACAAA AGATACCTTA 
TCACCTGGTG AAGTTTCAGC ATTGACCCCA AGGGAGAAAA TGCCTTTGCA GATGGAGGAG 
CTCAATACTG CAATTTATTC AAGTGATGGC TTACTTATTG CTAAGGAAGC AAAGATAAGA 
GATAGTGAAA CATTTTCAGA TTCATCTCCA ATTGAGATTA TAGATGAGTT CCCTACATTT 
GTCAGTTCCA AAACTGATTC TTCTAAATAT ACCAGGGAAT ACACTGACCT AGAAGTATCC 
CACAAAAGGG AAATTGCTAA TGTCCAGGAT GGAGCCGGCT CATTGCCTTG CTCTGAATTA 
CCCCAAGACC TTATTTTCAA GAATGTGCAA CATAAAGGTG AAGATAAAGC CCATGTCCCA 
GATGAAATCT CTAAAGATAG ATCTGATATT TCAAAGATGC CCTTACTGCC TCCAGATGCT 
TCTGCTCTGG CTGCTCAAAC AGAGATAGGT AGTATAGTTA AACCCAAAGT TCTTGTTAAA 
GAAGCTGAGA AAAAACTTCC TTCTGGTACA GAAAAAGAGA ACAGATCACC GTCTGCTATA 
TTTTCAGCAG AGCTGAGTAA AACTTCAGTG GTCGACCTCC TCTACTGGAG AGACATTAAG 
AAGACTGGAG TGGTGTTTGG TGCCAGCNNN NNNCTTCTCT TTTCATTGAC AGTATTCAGC 
ATTGTGAGTG TAACGGCCTA CATCGCCTTG GCCCTGCTCT CTGTGACTAT CAGCTTTAGG 
ATATATAAGG GTGTGATCCA AGCTATCCAG AAATCAGATG AAGGCCACCC ATTCAGGGCA 
TATTTGGAAT CTGAAGTTGC TATATCTGAG GAATTGGTTC AGAAATACAG TAATTCTGCT 
CTTGGTCATG TGAACTGCAC GATAAAAGAA CTCAGGCGCC TCTTCTTAGT TGACGATTTA 
GTTGATTCTC TGAAGTTTGC AGTGTTGATG TGGGTATTTA CCTATGTTGG TGCCTTGTTC 
AATGGTCTGA CACTACTGAT TTTGGCTCTG ATTTCACTCT TCAGCGTTCC TGTTATTTAT 
GAACGGCATC AGGCGCAGAT AGATCATTAC CTAGGACTTG CAAATAAGAA TGTTAAAGAT 
GCTATGGCTA AAATCCAAGC AAAAATCCCT GGATTGAAAC GCAAAGCTGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015442979.1
CDS	44..3397
Translation	MEDMEQSPLVSSSSESPPRPQPPFKYQFVQDSEDEEDEEPEDEEDEEPEEEDEDLEELEPPARPPPPPPAGVSSLAEPAWTPAAAGPAAPPSTPAAPKRRSSSGSVDETLFALPAASEPVIHSSAEKITDLKEQPGSTVSTGQEDFPSVLLETAASLPSLSPLSAASFKEHEYLGNLPAVLPTKGTLQETSDEASKEFSEQAKNPFVDRDLTEVSELEYSEMGSSFIVSPKAESAVTVADLREEIIVKNKDEEEDLVSNNILRNQQESPVTLTQLIKEENTVMSPEETKDSFKEKGLIAGAPMREEYADFKPFERVWEVKDIYKQGSDVLATAGSTESKLESKVDKKCFADSTEQTNHERDSESSNDDISFPSTPEAIKVGSGAYITCTPFNPKATEGISTNVFPLLEDHTSENKTDEKKIEEKKAQIVTEKNTSITSSNPFLVATQDSETGYVKTDNLSKMTEKVVANMPEGLTPDLVQEACGSELNETIGTKIAFETKMDLVQTSEAVQESLYPVAQLCPSFEESEATPSPVLPDIVMEAPLNSVVPSAGASAVQPSSSPLEAPLSVNYESMKLEPENPPPYEEAMNVSLKKVSGTKEEIKEPESINVAVQETEAPYISIACDLIKETKLSTEPTPDFSNYSEIAKVAQPAPDHSELVEDSSPDSEPVDLFSDDSIPEVPQKQDKAMIFVKENFVETSSELMTEHENKEKLGASPPPKAGKPYLESLQPSSDTTKDTLSPGEVSALTPREKMPLQMEELNTAIYSSDGLLIAKEAKIRDSETFSDSSPIEIIDEFPTFVSSKTDSSKYTREYTDLEVSHKREIANVQDGAGSLPCSELPQDLIFKNVQHKGEDKAHVPDEISKDRSDISKMPLLPPDASALAAQTEIGSIVKPKVLVKEAEKKLPSGTEKENRSPSAIFSAELSKTSVVDLLYWRDIKKTGVVFGASnullnullLLFSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNCTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFSVPVIYERHQAQIDHYLGLANKNVKDAMAKIQAKIPGLKRKAE

Target ORF information:

RefSeq Version	XM_015587493.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto reticulon 4 (RTN4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015587493.1

ORF Insert Sequence:

ATGGAAGACA TGGAGCAGTC GCCTCTGGTC TCCTCGTCCT CGGAAAGCCC TCCCCGGCCT 
CAGCCCCCGT TCAAGTACCA GTTCGTGCAG GATTCCGAGG ACGAAGAGGA CGAGGAGCCC 
GAGGACGAGG AGGACGAGGA GCCCGAGGAA GAGGACGAGG ACCTGGAGGA GCTGGAGCCT 
CCTGCCCGGC CTCCCCCGCC TCCTCCGGCC GGCGTGAGCT CCCTGGCCGA GCCCGCGTGG 
ACCCCGGCCG CCGCCGGCCC CGCCGCGCCC CCCTCCACCC CGGCCGCGCC CAAGCGCAGG 
AGCTCCTCAG GCTCTGTGGA TGAGACACTT TTTGCTCTTC CTGCTGCATC TGAGCCTGTG 
ATACACTCCT CTGCAGAAAA AATTACGGAC TTGAAGGAGC AGCCAGGTAG CACTGTTTCC 
ACTGGTCAAG AGGATTTCCC ATCTGTCCTG CTTGAAACTG CTGCTTCTCT TCCTTCTCTA 
TCTCCCCTCT CAGCTGCTTC TTTTAAAGAA CATGAATACC TTGGTAATTT ACCAGCAGTA 
TTACCCACCA AAGGAACACT TCAAGAAACT TCAGATGAAG CTTCTAAAGA GTTTTCAGAG 
CAGGCAAAAA ATCCATTTGT AGACAGAGAT TTAACTGAGG TTTCAGAATT GGAATATTCA 
GAAATGGGAT CATCATTCAT TGTCTCTCCA AAAGCAGAAT CTGCCGTAAC AGTCGCAGAC 
CTTAGGGAAG AAATAATTGT GAAGAATAAA GATGAAGAGG AGGATTTAGT TAGCAATAAC 
ATCCTTCGTA ATCAACAAGA GTCACCTGTA ACCCTTACTC AGTTGATTAA AGAAGAGAAC 
ACAGTCATGT CTCCAGAAGA AACAAAGGAC AGTTTTAAGG AAAAGGGACT CATAGCGGGA 
GCGCCTATGA GGGAAGAATA TGCAGACTTC AAACCATTTG AACGAGTATG GGAAGTAAAA 
GATATTTATA AGCAAGGTAG TGATGTGCTG GCTACTGCAG GTAGTACAGA GAGCAAGTTG 
GAAAGTAAAG TGGATAAGAA ATGTTTTGCA GATAGCACTG AGCAAACAAA TCATGAAAGA 
GATAGTGAAA GCAGTAATGA TGATATTTCT TTTCCCAGTA CACCGGAAGC TATAAAAGTT 
GGTTCAGGGG CATATATCAC ATGCACTCCC TTTAACCCAA AAGCAACTGA AGGCATTTCA 
ACAAATGTTT TTCCTTTGTT GGAAGATCAT ACTTCAGAAA ATAAGACAGA TGAAAAAAAA 
ATAGAAGAAA AAAAGGCCCA GATTGTAACA GAGAAGAATA CTAGCATTAC ATCTTCGAAC 
CCTTTCCTTG TAGCAACACA AGATTCTGAG ACAGGTTATG TCAAAACCGA TAATTTATCA 
AAGATGACTG AGAAAGTAGT GGCAAACATG CCTGAAGGTC TAACCCCAGA TTTAGTACAG 
GAAGCATGTG GAAGTGAATT GAATGAAACT ATAGGGACAA AGATTGCTTT TGAAACAAAA 
ATGGACTTGG TTCAAACATC AGAAGCTGTG CAGGAGTCAC TCTACCCTGT AGCACAGCTT 
TGCCCATCAT TTGAAGAATC TGAAGCTACT CCTTCGCCAG TTTTGCCTGA CATTGTTATG 
GAAGCACCAT TAAATTCTGT AGTTCCTAGT GCTGGTGCTT CTGCAGTGCA GCCCAGTTCA 
TCACCATTAG AAGCTCCCCT TTCAGTTAAT TATGAAAGCA TGAAACTTGA GCCTGAAAAT 
CCACCACCAT ATGAAGAGGC AATGAATGTA TCACTAAAAA AAGTATCAGG AACAAAGGAA 
GAAATTAAAG AGCCTGAGAG TATTAATGTA GCTGTTCAAG AAACAGAAGC TCCTTATATA 
TCTATTGCAT GTGATTTAAT TAAAGAAACA AAGCTCTCTA CTGAACCAAC TCCAGATTTC 
TCTAATTATT CAGAAATAGC AAAAGTTGCA CAGCCAGCGC CTGATCATTC TGAGCTTGTT 
GAAGATTCCT CACCTGATTC TGAACCAGTT GACTTATTTA GTGATGATTC AATACCTGAA 
GTTCCACAAA AACAAGATAA AGCTATGATA TTTGTGAAAG AGAATTTTGT TGAAACTTCA 
TCTGAGTTAA TGACAGAACA TGAAAATAAG GAAAAACTCG GTGCTTCACC ACCACCTAAG 
GCGGGGAAGC CATATTTGGA ATCTCTTCAG CCCAGTTCAG ACACCACAAA AGATACCTTA 
TCACCTGGTG AAGTTTCAGC ATTGACCCCA AGGGAGAAAA TGCCTTTGCA GATGGAGGAG 
CTCAATACTG CAATTTATTC AAGTGATGGC TTACTTATTG CTAAGGAAGC AAAGATAAGA 
GATAGTGAAA CATTTTCAGA TTCATCTCCA ATTGAGATTA TAGATGAGTT CCCTACATTT 
GTCAGTTCCA AAACTGATTC TTCTAAATAT ACCAGGGAAT ACACTGACCT AGAAGTATCC 
CACAAAAGGG AAATTGCTAA TGTCCAGGAT GGAGCCGGCT CATTGCCTTG CTCTGAATTA 
CCCCAAGACC TTATTTTCAA GAATGTGCAA CATAAAGGTG AAGATAAAGC CCATGTCCCA 
GATGAAATCT CTAAAGATAG ATCTGATATT TCAAAGATGC CCTTACTGCC TCCAGATGCT 
TCTGCTCTGG CTGCTCAAAC AGAGATAGGT AGTATAGTTA AACCCAAAGT TCTTGTTAAA 
GAAGCTGAGA AAAAACTTCC TTCTGGTACA GAAAAAGAGA ACAGATCACC GTCTGCTATA 
TTTTCAGCAG AGCTGAGTAA AACTTCAGTG GTCGACCTCC TCTACTGGAG AGACATTAAG 
AAGACTGGAG TGGTGTTTGG TGCCAGCNNN NNNCTTCTCT TTTCATTGAC AGTATTCAGC 
ATTGTGAGTG TAACGGCCTA CATCGCCTTG GCCCTGCTCT CTGTGACTAT CAGCTTTAGG 
ATATATAAGG GTGTGATCCA AGCTATCCAG AAATCAGATG AAGGCCACCC ATTCAGGGCA 
TATTTGGAAT CTGAAGTTGC TATATCTGAG GAATTGGTTC AGAAATACAG TAATTCTGCT 
CTTGGTCATG TGAACTGCAC GATAAAAGAA CTCAGGCGCC TCTTCTTAGT TGACGATTTA 
GTTGATTCTC TGAAGTTTGC AGTGTTGATG TGGGTATTTA CCTATGTTGG TGCCTTGTTC 
AATGGTCTGA CACTACTGAT TTTGGCTCTG ATTTCACTCT TCAGCGTTCC TGTTATTTAT 
GAACGGCATC AGGCGCAGAT AGATCATTAC CTAGGACTTG CAAATAAGAA TGTTAAAGAT 
GCTATGGCTA AAATCCAAGC AAAAATCCCT GGATTGAAAC GCAAAGCTGA ATGA