CRIM1 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRIM1
Entrez Gene ID	102888974
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006910382.3	XP_006910444.1	cysteine-rich motor neuron 1 protein
XM_006910382.2	XP_006910444.1	cysteine-rich motor neuron 1 protein

Pathways from BioSystems
Gene Ontology

The following CRIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt12660	XM_006910382.3 Latest version!	Pteropus alecto cysteine rich transmembrane BMP regulator 1 (CRIM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OPt12660	XM_006910382.2	Pteropus alecto cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRIM1 ( XM_006910382.3 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006910444.1 Pteropus alecto cysteine rich transmembrane BMP regulator 1 (CRIM1), mRNA.

Price & Availability↑

CloneID	OPt12660
Clone ID Related Accession (Same CDS sequence)	XM_006910382.3 , XM_006910382.2
Accession Version	XM_006910382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006434835.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_006910382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCTAGGAGC TGCCCCGGGA GCATCGTCCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGATCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACAGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAACTGCTT 
GGTGTTGAAC CATGTAATGA AAACCTTGTC TCTGGCTGCA ATATAATCAA TGGGAAATGT 
GAGTGTGGCA CCATTCGGAC CTGCAACAAT CCCTTTGAGT TTCCGAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGCGAA 
GTCCAGTTTT CTCCGCGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA CGCTCCTCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGT GTGTGCGACC CCGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAAG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTG TTCAGCGTGG ACTGCAGCAC TGTGGAGTGC 
CCCTCCGTAC AGCAGGCCGT GTGCCCCCTG GATAGCTACG AAACTCAAGT CAGACTCACG 
GCGGATGGTT GCTGCACTCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAT 
GTGGAGTATA ATGACGGAGA TATGTTTCGA ATGGACAACT GTCGGTTCTG CCGATGCCAA 
GGGGGCGTTT CCATCTGCTT CACTGCCCAG TGCGGGGAGC TGAACTGCGA GAGGTACTAC 
GTGCCTGAAG GGGAGTGCTG CCCGGTGTGC GAAGATCCAG TGTATCCTTT TAATAACCCT 
GCTGGCTGCT ATGCCAACGG CCAGATCCGC GCCCATGGAG ACCGTTGGCG GGAAGACGAC 
TGTACATTCT GCCAGTGCAT CAACGGAGAA CCCCACTGCG TTGCAACAGC CTGTGGACAG 
AGCTGCATGA ACCCCGTCAA AGTCCCTGGG GAGTGTTGCC CCGTGTGTGA AGAACCAACC 
TACATCACGA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACCTT GGTGGAGAAG 
GATTGCATTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACTTGTCA GTGCAAAAAC 
AGAGAGGAAC TGTGCACAGG CCTCAAGCGA GGCTGCAGCT TGGACTGCCC CTTCGGTTTC 
CTCACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGTC CACGGCCCAA GAAGTGCAGA 
CCCATAATCT GTGACAAGTA CTGTCCACTT GGATACCTGA AGAATAAGCA TGGCTGTGAC 
ATCTGTCGCT GTAAGAGATG CCCAGAGCTC CCATGCAGTA AAGTCTGTCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCCTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGTC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CAGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCGGGACA GTGCTGCCCG 
TCGTGTTCAG ATGACTTCGT GGTGCAGAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAC 
GCCCCTGGAG GAGAGTACTT TGTGGAAGGA GAAACATGGA ACATAGACTC CTGTACTCAG 
TGCACCTGCC ACAGCGGGCG GGTGCTGTGT GAAACAGAGG TGTGCCCGCC TCTACTCTGC 
CAGAACCCCT CGCGCACCCA GGACTCCTGC TGCCCGCAGT GCACAGATGA ACCTCTCCAG 
ACTCCCTCAT CTCGTAACGA CAGCGTGCCT AGCTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAGCCCGAC GTTTGTACCA GCTGCGTGTG CATGGATAGT 
GTAATCAGCT GTTACTCTGA GTCTTGCCCT TCAGTGTCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA CTGCATCGAA GACACAATTG CAAAGAAGAC GGTGTGCCAC 
TTCAGTGGGA AGGCCTACGC CGACGAGGAA CGCTGGGACA TCGATAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACCGTCAGTT GCCCGCCTCT GCCCTGTGTC 
GAGCCCATCA ATGTGGAAGG AAGTTGCTGC CCGATGTGTC CAGAAATGTA TGTCCCGGAA 
CCAACCAATA TACCCATTGA GAAGACAAAT CATCGTGGAG AGATTGACCT AGAGGTTCCT 
GTGTGGCCCA CACCTAGTGA AAATGACATC CATCTCCCCA GAGATATGGG TCACCTCCAG 
GTAGATTACA GAGATAATAA CAGACGGCCC CCAGGAGAAG ACTCTTCGCT GGACTCCATA 
GCCTCAGTCG TGGTTCCCAT CATAATAGGC CTTTCTATTA TAATAGCATT CCTATTCATC 
AATCAGAAGA AACAGTGGAT ACCCCTGCTT TGCTGGTATC GAACACCAAC TAAGCCTTCT 
TCCTTAAATA ATCAGCTGGT ATCTGTGGAC TGCAAGAAAG GAACCAGAGT CCAGGGGGAC 
AGTGCCCAGA GAATGCTAAG AATTGCTGAA CCAGATGCAA GATTCAGTGG CTTCTACAGC 
ATGCAAAAAC AGAATCATCT ACAGGCAGAC AATTTCTACC AAACGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006910444.1
CDS	5..3115
Translation	MYLVAGGRGLAGCGHLLVSLLGLLLLLARSGTRALVCLPCDESKCEEPRSCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGVEPCNENLVSGCNIINGKCECGTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLVEKDCIYGFKLDHNGCRTCQCKNREELCTGLKRGCSLDCPFGFLTDAHNCEICQCRPRPKKCRPIICDKYCPLGYLKNKHGCDICRCKRCPELPCSKVCPLGFQQDSHGCLICKCREVPASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCQNPSRTQDSCCPQCTDEPLQTPSSRNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIAKKTVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGEIDLEVPVWPTPSENDIHLPRDMGHLQVDYRDNNRRPPGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQGDSAQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006910382.3
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto cysteine rich transmembrane BMP regulator 1 (CRIM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006910382.3

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCTAGGAGC TGCCCCGGGA GCATCGTCCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGATCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACAGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAACTGCTT 
GGTGTTGAAC CATGTAATGA AAACCTTGTC TCTGGCTGCA ATATAATCAA TGGGAAATGT 
GAGTGTGGCA CCATTCGGAC CTGCAACAAT CCCTTTGAGT TTCCGAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGCGAA 
GTCCAGTTTT CTCCGCGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA CGCTCCTCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGT GTGTGCGACC CCGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAAG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTG TTCAGCGTGG ACTGCAGCAC TGTGGAGTGC 
CCCTCCGTAC AGCAGGCCGT GTGCCCCCTG GATAGCTACG AAACTCAAGT CAGACTCACG 
GCGGATGGTT GCTGCACTCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAT 
GTGGAGTATA ATGACGGAGA TATGTTTCGA ATGGACAACT GTCGGTTCTG CCGATGCCAA 
GGGGGCGTTT CCATCTGCTT CACTGCCCAG TGCGGGGAGC TGAACTGCGA GAGGTACTAC 
GTGCCTGAAG GGGAGTGCTG CCCGGTGTGC GAAGATCCAG TGTATCCTTT TAATAACCCT 
GCTGGCTGCT ATGCCAACGG CCAGATCCGC GCCCATGGAG ACCGTTGGCG GGAAGACGAC 
TGTACATTCT GCCAGTGCAT CAACGGAGAA CCCCACTGCG TTGCAACAGC CTGTGGACAG 
AGCTGCATGA ACCCCGTCAA AGTCCCTGGG GAGTGTTGCC CCGTGTGTGA AGAACCAACC 
TACATCACGA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACCTT GGTGGAGAAG 
GATTGCATTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACTTGTCA GTGCAAAAAC 
AGAGAGGAAC TGTGCACAGG CCTCAAGCGA GGCTGCAGCT TGGACTGCCC CTTCGGTTTC 
CTCACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGTC CACGGCCCAA GAAGTGCAGA 
CCCATAATCT GTGACAAGTA CTGTCCACTT GGATACCTGA AGAATAAGCA TGGCTGTGAC 
ATCTGTCGCT GTAAGAGATG CCCAGAGCTC CCATGCAGTA AAGTCTGTCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCCTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGTC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CAGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCGGGACA GTGCTGCCCG 
TCGTGTTCAG ATGACTTCGT GGTGCAGAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAC 
GCCCCTGGAG GAGAGTACTT TGTGGAAGGA GAAACATGGA ACATAGACTC CTGTACTCAG 
TGCACCTGCC ACAGCGGGCG GGTGCTGTGT GAAACAGAGG TGTGCCCGCC TCTACTCTGC 
CAGAACCCCT CGCGCACCCA GGACTCCTGC TGCCCGCAGT GCACAGATGA ACCTCTCCAG 
ACTCCCTCAT CTCGTAACGA CAGCGTGCCT AGCTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAGCCCGAC GTTTGTACCA GCTGCGTGTG CATGGATAGT 
GTAATCAGCT GTTACTCTGA GTCTTGCCCT TCAGTGTCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA CTGCATCGAA GACACAATTG CAAAGAAGAC GGTGTGCCAC 
TTCAGTGGGA AGGCCTACGC CGACGAGGAA CGCTGGGACA TCGATAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACCGTCAGTT GCCCGCCTCT GCCCTGTGTC 
GAGCCCATCA ATGTGGAAGG AAGTTGCTGC CCGATGTGTC CAGAAATGTA TGTCCCGGAA 
CCAACCAATA TACCCATTGA GAAGACAAAT CATCGTGGAG AGATTGACCT AGAGGTTCCT 
GTGTGGCCCA CACCTAGTGA AAATGACATC CATCTCCCCA GAGATATGGG TCACCTCCAG 
GTAGATTACA GAGATAATAA CAGACGGCCC CCAGGAGAAG ACTCTTCGCT GGACTCCATA 
GCCTCAGTCG TGGTTCCCAT CATAATAGGC CTTTCTATTA TAATAGCATT CCTATTCATC 
AATCAGAAGA AACAGTGGAT ACCCCTGCTT TGCTGGTATC GAACACCAAC TAAGCCTTCT 
TCCTTAAATA ATCAGCTGGT ATCTGTGGAC TGCAAGAAAG GAACCAGAGT CCAGGGGGAC 
AGTGCCCAGA GAATGCTAAG AATTGCTGAA CCAGATGCAA GATTCAGTGG CTTCTACAGC 
ATGCAAAAAC AGAATCATCT ACAGGCAGAC AATTTCTACC AAACGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_006910382.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006910444.1 Pteropus alecto cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), mRNA.

Price & Availability↑

CloneID	OPt12660
Clone ID Related Accession (Same CDS sequence)	XM_006910382.3 , XM_006910382.2
Accession Version	XM_006910382.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006434835.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006910382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCTAGGAGC TGCCCCGGGA GCATCGTCCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGATCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACAGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAACTGCTT 
GGTGTTGAAC CATGTAATGA AAACCTTGTC TCTGGCTGCA ATATAATCAA TGGGAAATGT 
GAGTGTGGCA CCATTCGGAC CTGCAACAAT CCCTTTGAGT TTCCGAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGCGAA 
GTCCAGTTTT CTCCGCGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA CGCTCCTCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGT GTGTGCGACC CCGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAAG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTG TTCAGCGTGG ACTGCAGCAC TGTGGAGTGC 
CCCTCCGTAC AGCAGGCCGT GTGCCCCCTG GATAGCTACG AAACTCAAGT CAGACTCACG 
GCGGATGGTT GCTGCACTCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAT 
GTGGAGTATA ATGACGGAGA TATGTTTCGA ATGGACAACT GTCGGTTCTG CCGATGCCAA 
GGGGGCGTTT CCATCTGCTT CACTGCCCAG TGCGGGGAGC TGAACTGCGA GAGGTACTAC 
GTGCCTGAAG GGGAGTGCTG CCCGGTGTGC GAAGATCCAG TGTATCCTTT TAATAACCCT 
GCTGGCTGCT ATGCCAACGG CCAGATCCGC GCCCATGGAG ACCGTTGGCG GGAAGACGAC 
TGTACATTCT GCCAGTGCAT CAACGGAGAA CCCCACTGCG TTGCAACAGC CTGTGGACAG 
AGCTGCATGA ACCCCGTCAA AGTCCCTGGG GAGTGTTGCC CCGTGTGTGA AGAACCAACC 
TACATCACGA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACCTT GGTGGAGAAG 
GATTGCATTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACTTGTCA GTGCAAAAAC 
AGAGAGGAAC TGTGCACAGG CCTCAAGCGA GGCTGCAGCT TGGACTGCCC CTTCGGTTTC 
CTCACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGTC CACGGCCCAA GAAGTGCAGA 
CCCATAATCT GTGACAAGTA CTGTCCACTT GGATACCTGA AGAATAAGCA TGGCTGTGAC 
ATCTGTCGCT GTAAGAGATG CCCAGAGCTC CCATGCAGTA AAGTCTGTCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCCTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGTC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CAGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCGGGACA GTGCTGCCCG 
TCGTGTTCAG ATGACTTCGT GGTGCAGAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAC 
GCCCCTGGAG GAGAGTACTT TGTGGAAGGA GAAACATGGA ACATAGACTC CTGTACTCAG 
TGCACCTGCC ACAGCGGGCG GGTGCTGTGT GAAACAGAGG TGTGCCCGCC TCTACTCTGC 
CAGAACCCCT CGCGCACCCA GGACTCCTGC TGCCCGCAGT GCACAGATGA ACCTCTCCAG 
ACTCCCTCAT CTCGTAACGA CAGCGTGCCT AGCTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAGCCCGAC GTTTGTACCA GCTGCGTGTG CATGGATAGT 
GTAATCAGCT GTTACTCTGA GTCTTGCCCT TCAGTGTCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA CTGCATCGAA GACACAATTG CAAAGAAGAC GGTGTGCCAC 
TTCAGTGGGA AGGCCTACGC CGACGAGGAA CGCTGGGACA TCGATAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACCGTCAGTT GCCCGCCTCT GCCCTGTGTC 
GAGCCCATCA ATGTGGAAGG AAGTTGCTGC CCGATGTGTC CAGAAATGTA TGTCCCGGAA 
CCAACCAATA TACCCATTGA GAAGACAAAT CATCGTGGAG AGATTGACCT AGAGGTTCCT 
GTGTGGCCCA CACCTAGTGA AAATGACATC CATCTCCCCA GAGATATGGG TCACCTCCAG 
GTAGATTACA GAGATAATAA CAGACGGCCC CCAGGAGAAG ACTCTTCGCT GGACTCCATA 
GCCTCAGTCG TGGTTCCCAT CATAATAGGC CTTTCTATTA TAATAGCATT CCTATTCATC 
AATCAGAAGA AACAGTGGAT ACCCCTGCTT TGCTGGTATC GAACACCAAC TAAGCCTTCT 
TCCTTAAATA ATCAGCTGGT ATCTGTGGAC TGCAAGAAAG GAACCAGAGT CCAGGGGGAC 
AGTGCCCAGA GAATGCTAAG AATTGCTGAA CCAGATGCAA GATTCAGTGG CTTCTACAGC 
ATGCAAAAAC AGAATCATCT ACAGGCAGAC AATTTCTACC AAACGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006910444.1
CDS	5..3115
Translation	MYLVAGGRGLAGCGHLLVSLLGLLLLLARSGTRALVCLPCDESKCEEPRSCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGVEPCNENLVSGCNIINGKCECGTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLVEKDCIYGFKLDHNGCRTCQCKNREELCTGLKRGCSLDCPFGFLTDAHNCEICQCRPRPKKCRPIICDKYCPLGYLKNKHGCDICRCKRCPELPCSKVCPLGFQQDSHGCLICKCREVPASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCQNPSRTQDSCCPQCTDEPLQTPSSRNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIAKKTVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGEIDLEVPVWPTPSENDIHLPRDMGHLQVDYRDNNRRPPGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQGDSAQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006910382.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006910382.2

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCTAGGAGC TGCCCCGGGA GCATCGTCCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGATCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACAGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAACTGCTT 
GGTGTTGAAC CATGTAATGA AAACCTTGTC TCTGGCTGCA ATATAATCAA TGGGAAATGT 
GAGTGTGGCA CCATTCGGAC CTGCAACAAT CCCTTTGAGT TTCCGAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGCGAA 
GTCCAGTTTT CTCCGCGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA CGCTCCTCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGT GTGTGCGACC CCGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAAG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTG TTCAGCGTGG ACTGCAGCAC TGTGGAGTGC 
CCCTCCGTAC AGCAGGCCGT GTGCCCCCTG GATAGCTACG AAACTCAAGT CAGACTCACG 
GCGGATGGTT GCTGCACTCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAT 
GTGGAGTATA ATGACGGAGA TATGTTTCGA ATGGACAACT GTCGGTTCTG CCGATGCCAA 
GGGGGCGTTT CCATCTGCTT CACTGCCCAG TGCGGGGAGC TGAACTGCGA GAGGTACTAC 
GTGCCTGAAG GGGAGTGCTG CCCGGTGTGC GAAGATCCAG TGTATCCTTT TAATAACCCT 
GCTGGCTGCT ATGCCAACGG CCAGATCCGC GCCCATGGAG ACCGTTGGCG GGAAGACGAC 
TGTACATTCT GCCAGTGCAT CAACGGAGAA CCCCACTGCG TTGCAACAGC CTGTGGACAG 
AGCTGCATGA ACCCCGTCAA AGTCCCTGGG GAGTGTTGCC CCGTGTGTGA AGAACCAACC 
TACATCACGA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACCTT GGTGGAGAAG 
GATTGCATTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACTTGTCA GTGCAAAAAC 
AGAGAGGAAC TGTGCACAGG CCTCAAGCGA GGCTGCAGCT TGGACTGCCC CTTCGGTTTC 
CTCACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGTC CACGGCCCAA GAAGTGCAGA 
CCCATAATCT GTGACAAGTA CTGTCCACTT GGATACCTGA AGAATAAGCA TGGCTGTGAC 
ATCTGTCGCT GTAAGAGATG CCCAGAGCTC CCATGCAGTA AAGTCTGTCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCCTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGTC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CAGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCGGGACA GTGCTGCCCG 
TCGTGTTCAG ATGACTTCGT GGTGCAGAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAC 
GCCCCTGGAG GAGAGTACTT TGTGGAAGGA GAAACATGGA ACATAGACTC CTGTACTCAG 
TGCACCTGCC ACAGCGGGCG GGTGCTGTGT GAAACAGAGG TGTGCCCGCC TCTACTCTGC 
CAGAACCCCT CGCGCACCCA GGACTCCTGC TGCCCGCAGT GCACAGATGA ACCTCTCCAG 
ACTCCCTCAT CTCGTAACGA CAGCGTGCCT AGCTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAGCCCGAC GTTTGTACCA GCTGCGTGTG CATGGATAGT 
GTAATCAGCT GTTACTCTGA GTCTTGCCCT TCAGTGTCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA CTGCATCGAA GACACAATTG CAAAGAAGAC GGTGTGCCAC 
TTCAGTGGGA AGGCCTACGC CGACGAGGAA CGCTGGGACA TCGATAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACCGTCAGTT GCCCGCCTCT GCCCTGTGTC 
GAGCCCATCA ATGTGGAAGG AAGTTGCTGC CCGATGTGTC CAGAAATGTA TGTCCCGGAA 
CCAACCAATA TACCCATTGA GAAGACAAAT CATCGTGGAG AGATTGACCT AGAGGTTCCT 
GTGTGGCCCA CACCTAGTGA AAATGACATC CATCTCCCCA GAGATATGGG TCACCTCCAG 
GTAGATTACA GAGATAATAA CAGACGGCCC CCAGGAGAAG ACTCTTCGCT GGACTCCATA 
GCCTCAGTCG TGGTTCCCAT CATAATAGGC CTTTCTATTA TAATAGCATT CCTATTCATC 
AATCAGAAGA AACAGTGGAT ACCCCTGCTT TGCTGGTATC GAACACCAAC TAAGCCTTCT 
TCCTTAAATA ATCAGCTGGT ATCTGTGGAC TGCAAGAAAG GAACCAGAGT CCAGGGGGAC 
AGTGCCCAGA GAATGCTAAG AATTGCTGAA CCAGATGCAA GATTCAGTGG CTTCTACAGC 
ATGCAAAAAC AGAATCATCT ACAGGCAGAC AATTTCTACC AAACGGTGTG A