TOP1 cDNA ORF clone, Pterocles gutturalis(yellow-throated sandgrouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP1
Entrez Gene ID	104470632
Full Name	DNA topoisomerase I
Gene Type	protein-coding
Organism	Pterocles gutturalis(yellow-throated sandgrouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010085622.1	XP_010083924.1	DNA topoisomerase 1

Pathways from BioSystems
Gene Ontology

The following TOP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt39710	XM_010085622.1 Latest version!	Pterocles gutturalis topoisomerase (DNA) I (TOP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP1 ( XM_010085622.1 ) cDNA ORF clone, Pterocles gutturalis(yellow-throated sandgrouse) -> XP_010083924.1 Pterocles gutturalis topoisomerase (DNA) I (TOP1), mRNA.

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CloneID	OPt39710
Clone ID Related Accession (Same CDS sequence)	XM_010085622.1
Accession Version	XM_010085622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Pterocles gutturalis(yellow-throated sandgrouse)
Product	DNA topoisomerase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010146111.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pterocles gutturalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAG CTTCTGGTGA TATAAAAATA AAGAAGGAAA AGGAAAATGG TTTCTCCAGT 
CCGCCACGTA TTAAAGATGA ACCAGATGAT GATGGGGTTT ATGCATCTCC TAAAGAGGAC 
TCCAAACCTC TGAAGAGACC TCGAGAAGAT GATGATGCTG AATACAAACC CAAGAAAATC 
AAAACAGAAG ACATAAAGAA AGCAAAGAAA AGGAAACAAG AGGAAGAAGA GGACAGCAAA 
GCAAAGAAAG CTAAAAGCAA AGATAAGAAA GTCCCTGAAG CAGACAAGAA AAAGAAACCG 
AAAAAAGAAG AGGAGCAAAA ATGGAAATGG TGGGAAGAAG AGCGCTACCC TGAAGGCATT 
AAATGGAAGT TCCTAGAGCA CAAAGGTCCT GTGTTTGCTC CACCATATGA ACCCCTGCCT 
GAAAATGTCA AGTTTTATTA TGATGGTAAA GTCATGAAGC TGAGTACCAA AGCAGAAGAA 
GTTGCCACAT TCTTTGCAAA AATGCTTGAT CATGAGTACA CTACAAAGGA GATCTTCAGG 
AAAAACTTTT TCAAAGACTG GAGAAAGGAA ATGACTCCCG AAGAGAAGAG CACTATCACC 
AGCCTCAGCA AGTGTGACTT CACCCACATG AGCCAGTATT TCAAAGCTCA GACGGAAGCT 
AGGAAACAGA TGTCCAAGGA GGAGAAACAG AAAATCAAAG AGGAGAATGA GCGGCTATTG 
AAGGAATATG GTTACTGTGT GATGGACAAC CACAAGGAGA GGATTGCCAA CTTTAAAATT 
GAGCCCCCAG GTCTCTTCCG AGGTCGTGGG AACCATCCTA AAATGGGCAT GTTGAAGAGA 
CGCATCATGC CAGAAGACAT CATCATAAAC TGCAGCAAGG ATTCCAAGAT CCCTGCCCCT 
CCACCAGGAC ACAAATGGAA GGAGGTCCGG CATGATAACA AGGTTACCTG GCTGGTGTCG 
TGGACTGAGA ACATCCAAGG CTCTATCAAA TACATCATGT TGAACCCTAG CTCAAGGATT 
AAGGGTGAGA AGGACTGGCA GAAGTATGAG ACAGCTCGGA GGTTGAAGAA GTGCGTAGAT 
AAAATCCGAA ATCAATATCG AGAAGACTGG AAATCTAAAG AGATGAAAGT GCGGCAGAGG 
GCTGTGGCAC TGTACTTCAT TGATAAGCTT GCCCTGAGAG CTGGTAATGA AAAAGAAGAA 
GGAGAGACAG CTGACACTGT GGGCTGCTGC TCTCTGAGAG TAGAACATAT CAAGCTGCAT 
CCTGAACTGG ATGGCCAGGA ATACGTGGTT GAGTTCGACT TCCTCGGAAA AGACTCCATC 
CGATACTACA ACAAAGTCCC TGTTGAGAAG AGGGTGTTTA AGAATCTTCA GCTGTTCATG 
GAGAACAAGC AGCCTGAGGA TGACCTCTTT GACCGCCTCA ATACCAGTAT CTTAAATAAA 
CATCTTCAAG ACCTTATGGA GGGACTGACA GCCAAGGTAT TCCGTACTTA CAACGCCTCC 
ATCACGCTAC AGCAGCAGCT CAAGGAGCTC ACTAATCCGG ACGACAACAT CCCAGCGAAG 
ATTCTTTCCT ATAACCGTGC CAACAGAGCG GTGGCCATCT TGTGTAACCA CCAGAGGGCT 
CCACCGAAGA CCTTCGAGAA GTCCATGATG AACCTGCAGA GCAAGATCGA TGCCAAGAAG 
GAGCAGTTAG CTGATGCCAG GAGAGAACTG AAAAGCGCCA AAGCTGATGC CAAGGTCCGG 
AGGGATGAGA AGTCTAAAAA GACAGTGGAG AGCAAGAAGA AAGCGGTGCA GAGGATTGAG 
GAGCAACTGA TGAAGCTGGA GGTTCAGGCT ACAGATAGGG AGGAGAACAA GCAGATCGCT 
TTGGGCACCT CCAAACTCAA CTATCTGGAT CCCAGGATCT CTGTTGCTTG GTGTAAGAAG 
TGGGGAATTC CTATAGAGAA GATTTATAAC AAAACCCAAC GAGAAAAATT TGCCTGGGCT 
ATCGACATGG CAGAGGAAGA CTATGAGTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010083924.1
CDS	226..2238
Translation	MKLASGDIKIKKEKENGFSSPPRIKDEPDDDGVYASPKEDSKPLKRPREDDDAEYKPKKIKTEDIKKAKKRKQEEEEDSKAKKAKSKDKKVPEADKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSTKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTPEEKSTITSLSKCDFTHMSQYFKAQTEARKQMSKEEKQKIKEENERLLKEYGYCVMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDSKIPAPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTSILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTNPDDNIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKEQLADARRELKSAKADAKVRRDEKSKKTVESKKKAVQRIEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKWGIPIEKIYNKTQREKFAWAIDMAEEDYEF

Target ORF information:

RefSeq Version	XM_010085622.1
Organism	Pterocles gutturalis(yellow-throated sandgrouse)
Definition	Pterocles gutturalis topoisomerase (DNA) I (TOP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010085622.1

ORF Insert Sequence:

ATGAAGTTAG CTTCTGGTGA TATAAAAATA AAGAAGGAAA AGGAAAATGG TTTCTCCAGT 
CCGCCACGTA TTAAAGATGA ACCAGATGAT GATGGGGTTT ATGCATCTCC TAAAGAGGAC 
TCCAAACCTC TGAAGAGACC TCGAGAAGAT GATGATGCTG AATACAAACC CAAGAAAATC 
AAAACAGAAG ACATAAAGAA AGCAAAGAAA AGGAAACAAG AGGAAGAAGA GGACAGCAAA 
GCAAAGAAAG CTAAAAGCAA AGATAAGAAA GTCCCTGAAG CAGACAAGAA AAAGAAACCG 
AAAAAAGAAG AGGAGCAAAA ATGGAAATGG TGGGAAGAAG AGCGCTACCC TGAAGGCATT 
AAATGGAAGT TCCTAGAGCA CAAAGGTCCT GTGTTTGCTC CACCATATGA ACCCCTGCCT 
GAAAATGTCA AGTTTTATTA TGATGGTAAA GTCATGAAGC TGAGTACCAA AGCAGAAGAA 
GTTGCCACAT TCTTTGCAAA AATGCTTGAT CATGAGTACA CTACAAAGGA GATCTTCAGG 
AAAAACTTTT TCAAAGACTG GAGAAAGGAA ATGACTCCCG AAGAGAAGAG CACTATCACC 
AGCCTCAGCA AGTGTGACTT CACCCACATG AGCCAGTATT TCAAAGCTCA GACGGAAGCT 
AGGAAACAGA TGTCCAAGGA GGAGAAACAG AAAATCAAAG AGGAGAATGA GCGGCTATTG 
AAGGAATATG GTTACTGTGT GATGGACAAC CACAAGGAGA GGATTGCCAA CTTTAAAATT 
GAGCCCCCAG GTCTCTTCCG AGGTCGTGGG AACCATCCTA AAATGGGCAT GTTGAAGAGA 
CGCATCATGC CAGAAGACAT CATCATAAAC TGCAGCAAGG ATTCCAAGAT CCCTGCCCCT 
CCACCAGGAC ACAAATGGAA GGAGGTCCGG CATGATAACA AGGTTACCTG GCTGGTGTCG 
TGGACTGAGA ACATCCAAGG CTCTATCAAA TACATCATGT TGAACCCTAG CTCAAGGATT 
AAGGGTGAGA AGGACTGGCA GAAGTATGAG ACAGCTCGGA GGTTGAAGAA GTGCGTAGAT 
AAAATCCGAA ATCAATATCG AGAAGACTGG AAATCTAAAG AGATGAAAGT GCGGCAGAGG 
GCTGTGGCAC TGTACTTCAT TGATAAGCTT GCCCTGAGAG CTGGTAATGA AAAAGAAGAA 
GGAGAGACAG CTGACACTGT GGGCTGCTGC TCTCTGAGAG TAGAACATAT CAAGCTGCAT 
CCTGAACTGG ATGGCCAGGA ATACGTGGTT GAGTTCGACT TCCTCGGAAA AGACTCCATC 
CGATACTACA ACAAAGTCCC TGTTGAGAAG AGGGTGTTTA AGAATCTTCA GCTGTTCATG 
GAGAACAAGC AGCCTGAGGA TGACCTCTTT GACCGCCTCA ATACCAGTAT CTTAAATAAA 
CATCTTCAAG ACCTTATGGA GGGACTGACA GCCAAGGTAT TCCGTACTTA CAACGCCTCC 
ATCACGCTAC AGCAGCAGCT CAAGGAGCTC ACTAATCCGG ACGACAACAT CCCAGCGAAG 
ATTCTTTCCT ATAACCGTGC CAACAGAGCG GTGGCCATCT TGTGTAACCA CCAGAGGGCT 
CCACCGAAGA CCTTCGAGAA GTCCATGATG AACCTGCAGA GCAAGATCGA TGCCAAGAAG 
GAGCAGTTAG CTGATGCCAG GAGAGAACTG AAAAGCGCCA AAGCTGATGC CAAGGTCCGG 
AGGGATGAGA AGTCTAAAAA GACAGTGGAG AGCAAGAAGA AAGCGGTGCA GAGGATTGAG 
GAGCAACTGA TGAAGCTGGA GGTTCAGGCT ACAGATAGGG AGGAGAACAA GCAGATCGCT 
TTGGGCACCT CCAAACTCAA CTATCTGGAT CCCAGGATCT CTGTTGCTTG GTGTAAGAAG 
TGGGGAATTC CTATAGAGAA GATTTATAAC AAAACCCAAC GAGAAAAATT TGCCTGGGCT 
ATCGACATGG CAGAGGAAGA CTATGAGTTT TAA