DBT cDNA ORF clone, Protobothrops mucrosquamatus

Gene
Clones
gRNAs

Summary

Gene Symbol	DBT
Entrez Gene ID	107302494
Full Name	dihydrolipoamide branched chain transacylase E2
Gene Type	protein-coding
Organism	Protobothrops mucrosquamatus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015831602.1	XP_015687088.1	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial

Pathways from BioSystems
Gene Ontology

The following DBT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DBT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr24340	XM_015831602.1 Latest version!	Protobothrops mucrosquamatus dihydrolipoamide branched chain transacylase E2 (DBT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DBT ( XM_015831602.1 ) cDNA ORF clone, Protobothrops mucrosquamatus -> XP_015687088.1 Protobothrops mucrosquamatus dihydrolipoamide branched chain transacylase E2 (DBT), mRNA.

Price & Availability↑

CloneID	OPr24340
Clone ID Related Accession (Same CDS sequence)	XM_015831602.1
Accession Version	XM_015831602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-22
Organism	Protobothrops mucrosquamatus
Product	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015386343.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Protobothrops mucrosquamatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCAA CGATTGCATT GAGGAGCAGC TGCAGAGCAG TCCGGCGTTT GCTCTGTGTT 
CACCAGATAA GACTACATAG TAACTTTTGT TTGCTAAAGC AAAAATACAT TTCTAAGTTT 
GAGAAGACTT TATGGAGGTA TCAACATCAG CATCAATTAT TTAGAACAAC TGCTGTTTCA 
CACGGCCCAA TTGTTCAGTT CAAGCTCTCT GATATTGGAG AAGGGATCAC AGAAGTGACA 
GTGAAAGAGT GGTATGTAAA AGAAGGTGAT GTTGTGTCAC AGTTTGATAG CATCTGTGAA 
GTACAAAGTG ATAAAGCCTC GGTCACAATC ACCAGCCGTT ACGATGGCAT CATTAGAAAA 
CTCCATTATG AGTTGGATGA AATTGCCCGT GTGGGGAAAC CACTGGTGGA TATAGAAACA 
GCTACTATAA AAGCTGTAAC TCCTGAGGAA GATGTGGTGG AAACACTTGC TGTGTCACGT 
GAAGAACAGA TCCACCAGCA AATCAAAGGC CATAAGACTC TAGTAACCCC TGCAGTTCGC 
CGCCTTGCTA TGGAAAACAA TATAAAGCTG AGTGAAGTTG TTGGAACAGG GAAAGATAAC 
CGTATTCTCA AAGAAGACAT TCTTAACTAC TTAGCCAAGC AGACTGGAGC AATTTTACCT 
CTGTCACCAA AGCCTGAGAT CATTCCACCT TCATCAAAAC CAGAACTTGC TTCAGACATA 
TCAAAAGAGA AAGCTGAAAG AACTGTAACA CCACTTTCTA AACCTGTAAT CTCAGCGAAA 
GATCGAACAA TGGCCTTATC TGGATTTCAG AAGGCAATGG TCAAAACTAT GAATGCTGCT 
TTGAAAATTC CTCACTTTGG TTATTGTGAT GAGATTGACC TCACTCGGCT CATCCATCTG 
AGAGAAGAGT TGAAGTCTTT AGCAAAAGAA CGGGGAGTTA AACTCACTTT TATGCCCTTC 
TTTTTAAAGG CCGCTTCTCT AAGCTTATTA CATTATCCCA TTCTTAATGC TTCTGTGGAT 
GAAGACTGCC AAAACATCAC ATACAAGGCT TCTCACAATA TTGGAATTGC CATGGACACA 
GGACTAGGCT TGCTCGTCCC TAATGTGAAA AATGTCCAGG CTTGTAGTGT GTTTGAAGTT 
GCTTCAGAAT TAAATCGCCT TCAAAAATTG GGCTCAGCTA ACCAGTTGGG AACAAATGAC 
CTTACAGGGG GAACATTCAC ACTTTCCAAC ATTGGCACAA TTGGTGGCAC TTATGCAAAA 
CCCGTGATCC TTCCTCCTGA AGTAGCTATT GGTGCTCTGG GAAAAATACA GGCCCTTCCT 
CGATTTAACA ATAAAGGTGA AGTATTTAAA GTACAAATAA TGAATGTGAG CTGGTCAGCT 
GATCACCGAA TCATTGATGG TGCAACCATG TCTCGTTTTT CTAACTTGTG GAAATCCTAC 
TTGGAGAATC CTGCTTCCAT GCTGATGGAT CTTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015687088.1
CDS	9..1487
Translation	MAATIALRSSCRAVRRLLCVHQIRLHSNFCLLKQKYISKFEKTLWRYQHQHQLFRTTAVSHGPIVQFKLSDIGEGITEVTVKEWYVKEGDVVSQFDSICEVQSDKASVTITSRYDGIIRKLHYELDEIARVGKPLVDIETATIKAVTPEEDVVETLAVSREEQIHQQIKGHKTLVTPAVRRLAMENNIKLSEVVGTGKDNRILKEDILNYLAKQTGAILPLSPKPEIIPPSSKPELASDISKEKAERTVTPLSKPVISAKDRTMALSGFQKAMVKTMNAALKIPHFGYCDEIDLTRLIHLREELKSLAKERGVKLTFMPFFLKAASLSLLHYPILNASVDEDCQNITYKASHNIGIAMDTGLGLLVPNVKNVQACSVFEVASELNRLQKLGSANQLGTNDLTGGTFTLSNIGTIGGTYAKPVILPPEVAIGALGKIQALPRFNNKGEVFKVQIMNVSWSADHRIIDGATMSRFSNLWKSYLENPASMLMDLK

Target ORF information:

RefSeq Version	XM_015831602.1
Organism	Protobothrops mucrosquamatus
Definition	Protobothrops mucrosquamatus dihydrolipoamide branched chain transacylase E2 (DBT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015831602.1

ORF Insert Sequence:

ATGGCGGCAA CGATTGCATT GAGGAGCAGC TGCAGAGCAG TCCGGCGTTT GCTCTGTGTT 
CACCAGATAA GACTACATAG TAACTTTTGT TTGCTAAAGC AAAAATACAT TTCTAAGTTT 
GAGAAGACTT TATGGAGGTA TCAACATCAG CATCAATTAT TTAGAACAAC TGCTGTTTCA 
CACGGCCCAA TTGTTCAGTT CAAGCTCTCT GATATTGGAG AAGGGATCAC AGAAGTGACA 
GTGAAAGAGT GGTATGTAAA AGAAGGTGAT GTTGTGTCAC AGTTTGATAG CATCTGTGAA 
GTACAAAGTG ATAAAGCCTC GGTCACAATC ACCAGCCGTT ACGATGGCAT CATTAGAAAA 
CTCCATTATG AGTTGGATGA AATTGCCCGT GTGGGGAAAC CACTGGTGGA TATAGAAACA 
GCTACTATAA AAGCTGTAAC TCCTGAGGAA GATGTGGTGG AAACACTTGC TGTGTCACGT 
GAAGAACAGA TCCACCAGCA AATCAAAGGC CATAAGACTC TAGTAACCCC TGCAGTTCGC 
CGCCTTGCTA TGGAAAACAA TATAAAGCTG AGTGAAGTTG TTGGAACAGG GAAAGATAAC 
CGTATTCTCA AAGAAGACAT TCTTAACTAC TTAGCCAAGC AGACTGGAGC AATTTTACCT 
CTGTCACCAA AGCCTGAGAT CATTCCACCT TCATCAAAAC CAGAACTTGC TTCAGACATA 
TCAAAAGAGA AAGCTGAAAG AACTGTAACA CCACTTTCTA AACCTGTAAT CTCAGCGAAA 
GATCGAACAA TGGCCTTATC TGGATTTCAG AAGGCAATGG TCAAAACTAT GAATGCTGCT 
TTGAAAATTC CTCACTTTGG TTATTGTGAT GAGATTGACC TCACTCGGCT CATCCATCTG 
AGAGAAGAGT TGAAGTCTTT AGCAAAAGAA CGGGGAGTTA AACTCACTTT TATGCCCTTC 
TTTTTAAAGG CCGCTTCTCT AAGCTTATTA CATTATCCCA TTCTTAATGC TTCTGTGGAT 
GAAGACTGCC AAAACATCAC ATACAAGGCT TCTCACAATA TTGGAATTGC CATGGACACA 
GGACTAGGCT TGCTCGTCCC TAATGTGAAA AATGTCCAGG CTTGTAGTGT GTTTGAAGTT 
GCTTCAGAAT TAAATCGCCT TCAAAAATTG GGCTCAGCTA ACCAGTTGGG AACAAATGAC 
CTTACAGGGG GAACATTCAC ACTTTCCAAC ATTGGCACAA TTGGTGGCAC TTATGCAAAA 
CCCGTGATCC TTCCTCCTGA AGTAGCTATT GGTGCTCTGG GAAAAATACA GGCCCTTCCT 
CGATTTAACA ATAAAGGTGA AGTATTTAAA GTACAAATAA TGAATGTGAG CTGGTCAGCT 
GATCACCGAA TCATTGATGG TGCAACCATG TCTCGTTTTT CTAACTTGTG GAAATCCTAC 
TTGGAGAATC CTGCTTCCAT GCTGATGGAT CTTAAGTGA