LOC105140472 cDNA ORF clone, Populus euphratica(Euphrates poplar)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105140472
Entrez Gene ID	105140472
Full Name	probable methionine--tRNA ligase
Gene Type	protein-coding
Organism	Populus euphratica(Euphrates poplar)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011047319.1	XP_011045621.1	probable methionine--tRNA ligase

Pathways from BioSystems
Gene Ontology

The following LOC105140472 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105140472 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo285099	XM_011047319.1 Latest version!	Populus euphratica probable methionine--tRNA ligase (LOC105140472), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105140472 ( XM_011047319.1 ) cDNA ORF clone, Populus euphratica(Euphrates poplar) -> XP_011045621.1 Populus euphratica probable methionine--tRNA ligase (LOC105140472), mRNA.

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CloneID	OPo285099
Clone ID Related Accession (Same CDS sequence)	XM_011047319.1
Accession Version	XM_011047319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-05
Organism	Populus euphratica(Euphrates poplar)
Product	probable methionine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011499993.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Populus euphratica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATC TCAATCGGAC GGCCCCGAAG CTACCTATCC CGGGAAAGCG AAACATACTG 
ATAACCAGTG CTTTGCCTTA TGTCAATAAC GTCCCTCATC TCGGGAACAT CATCGGCTGT 
GTGTTGAGTG CTGATGTTTT TGCTCGATAC TGTCGTCTTC GAGGATATAA CGCGATTTAT 
ATTTGTGGAA CTGACGAGTA TGGGACTGCG ACTGAGACTA AAGCCATGGA GGAGAATTGC 
ACTCCTAAAC AAATTTGCGA CAAGTATCAT GCAATTCATA GGGAGGTTTA TAAATGGTTT 
AATATAAGTT TTGATGAATT TGGACGTACG TCGAGTCCGC AGCAAACGGA AATTTGTCAA 
GCAATTTTTA AGAAATTGTT AGAGAATAAC TGGCTTTCGG AGAACTCAAT GCAACAGCTG 
TACTGTGATA CATGCGAAAG ATTCTTAGCT GATCGGCTTG TGGAAGGTAA GTGTCCAACA 
GAAGGATGTC ATTATGATTC TGCACGAGGC GATCAGTGTG AAAATTGTGG AAAGCTTTTG 
AATCCTACAG AGTTAATAAA TCCCAGGTGC AAGCAATGTA AAAACATACC ACGAATCCGT 
GATACAAATC ACTTGTTTCT TGAGCTTCCT CTACTGAAGG ATAAATTAGT ACACTATATC 
GAGAGCACAT CTGTAGCTGG GGGCTGGAGC CAAAATGCTA TTCAAACAAC AAATGCATGG 
CTTAAAGAAG GATTAAAACC ACGATGCATT ACCAGGGATC TGAAGTGGGG TGTTCCCGTC 
CCACTTGAAG AATTTAAGGA CAAGGTGTTC TACGTTTGGT TTGATGCTCC TATTGGATAT 
GTCTCCATTA CTTCTTGCTA CACTCCTGAT TGGGAGAAGT GGTGGAAGAA TCCTGAGAAT 
GTTGAGCTGT ATCAGTTTAT GGGAAAAGAC AATGTGCCAT TCCACACTGT GATGTTTCCT 
TCTACGCTTC TTGGAACAGG TGAAAATTGG ACTTTGATGA AGACTATAAG TGTAACTGAA 
TACTTAAATT ATGAAGCAGG AAAGTTTTCC AAGAGTAAAG GTGTAGGAGT GTTTGGTAAT 
GATGCAAAGG ATACAAATAT TCCTGTAGAA GTTTGGAGAT ATTATTTGTT AACCAATAGG 
CCTGAGGTGT CTGACACACT ATTTACATGG TCTGATTTGC AAGCAAAAAG TAACAATGAG 
TTGCTGAATA ATTTGGGCAA CTTTATTAAC CGAGTTCTGA GCTTTCTTGC TAAACCTCCA 
GGTTCAGGAT ATGGTTCTGT TATCCCAGAT GCTCCAGGTG CAGGCTCTCA TCCCCTGACA 
AATAAATTGG CTGGAGAAGT TGGTAAATAT GTGGAGCAAT ACCTGGAAGC CATGGAAAAG 
GTTAAATTAA AGCAGGGATT GAAAACTGCA ATGAGCATTT CTAGCGAGGG AAATGCATAT 
CTGCAAGAAA GCCAATTCTG GAGGCTTTAC AAGGAAGACC AACCTTCTTG TTCTATAGTT 
ATAAAGACTT CTTTGGGTCT AGTATATCTT CTTGCATGTT TGTTAGAACC TTTCATGCCA 
TCTTTCTCTG TTGAGGTCTT CAAGCAGCTT AATATGCTCC CTGAACAAGC TTCACTATGT 
GATGAGAAAG GTGATATGGA TAAGGCAAAA AGGCCTTGGG AGATCCTACC TGCTGGGCAC 
AAGATTGGGA CACCAGAGCC CTTGTTCAAG GAACTGAAAA TAGAAGAAGT GGAGGACTAC 
AGGAAGAAAT TTGCTGGCAG TCAGGCTGAC AGACTTGAGC GGGAAGAAGT TGAAAAGGCA 
TCTAAGCTTG CTGAGGAACT GAAGAAAAAA GCTACAGTTG GTGGGAAGAA GCAGCTGGCC 
AAAAAACCTG CTGGTGAAGC CAAATCAAGG GGCGCTGTTG AACCAGAAAT CTCCATCACA 
AGACTTGATA TTCGTGTTGG CCTAATTAAG AAAGCTCAGA AGCATCCTGA TGCTGATTCC 
CTGTATGTGG AAGAAATCGA CTTGGGTGAA GGAGATTGTA GAACGGTTGT AAGTGGACTT 
GTCAAGTATA TACCTCTTGA GGAGATGCAA AATCGGAAGG TCTGCATCCT TTGCAACCTA 
AAGCCTGCAA ATATGAGGGG CATCAAGTCA GAGGCTATGG TTCTTGCTGC TTCCAGTAGT 
GACCACACCA AGGTCGAATT GGTTGATCCA CCCCAATCTG CCGTTGTTGG AGAGAGAGTG 
ACATTCCCAG GGTTTGAAGG TGAACCTGAC GATGTCCTAA ACCCTAAGAA GAAAGTCTGG 
GAGGCACTAC AGGTGGATCT GCAAACTAAC AGTGACCTGG TAGCATGCTA CAAAGATATT 
CCACTAACCA CATCTGCTGG TGTGTGCAAG GTTGCAACCA TTGGCAATGG TTCCATTAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011045621.1
CDS	317..2719
Translation	MEDLNRTAPKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDEYGTATETKAMEENCTPKQICDKYHAIHREVYKWFNISFDEFGRTSSPQQTEICQAIFKKLLENNWLSENSMQQLYCDTCERFLADRLVEGKCPTEGCHYDSARGDQCENCGKLLNPTELINPRCKQCKNIPRIRDTNHLFLELPLLKDKLVHYIESTSVAGGWSQNAIQTTNAWLKEGLKPRCITRDLKWGVPVPLEEFKDKVFYVWFDAPIGYVSITSCYTPDWEKWWKNPENVELYQFMGKDNVPFHTVMFPSTLLGTGENWTLMKTISVTEYLNYEAGKFSKSKGVGVFGNDAKDTNIPVEVWRYYLLTNRPEVSDTLFTWSDLQAKSNNELLNNLGNFINRVLSFLAKPPGSGYGSVIPDAPGAGSHPLTNKLAGEVGKYVEQYLEAMEKVKLKQGLKTAMSISSEGNAYLQESQFWRLYKEDQPSCSIVIKTSLGLVYLLACLLEPFMPSFSVEVFKQLNMLPEQASLCDEKGDMDKAKRPWEILPAGHKIGTPEPLFKELKIEEVEDYRKKFAGSQADRLEREEVEKASKLAEELKKKATVGGKKQLAKKPAGEAKSRGAVEPEISITRLDIRVGLIKKAQKHPDADSLYVEEIDLGEGDCRTVVSGLVKYIPLEEMQNRKVCILCNLKPANMRGIKSEAMVLAASSSDHTKVELVDPPQSAVVGERVTFPGFEGEPDDVLNPKKKVWEALQVDLQTNSDLVACYKDIPLTTSAGVCKVATIGNGSIR

Target ORF information:

RefSeq Version	XM_011047319.1
Organism	Populus euphratica(Euphrates poplar)
Definition	Populus euphratica probable methionine--tRNA ligase (LOC105140472), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011047319.1

ORF Insert Sequence:

ATGGAAGATC TCAATCGGAC GGCCCCGAAG CTACCTATCC CGGGAAAGCG AAACATACTG 
ATAACCAGTG CTTTGCCTTA TGTCAATAAC GTCCCTCATC TCGGGAACAT CATCGGCTGT 
GTGTTGAGTG CTGATGTTTT TGCTCGATAC TGTCGTCTTC GAGGATATAA CGCGATTTAT 
ATTTGTGGAA CTGACGAGTA TGGGACTGCG ACTGAGACTA AAGCCATGGA GGAGAATTGC 
ACTCCTAAAC AAATTTGCGA CAAGTATCAT GCAATTCATA GGGAGGTTTA TAAATGGTTT 
AATATAAGTT TTGATGAATT TGGACGTACG TCGAGTCCGC AGCAAACGGA AATTTGTCAA 
GCAATTTTTA AGAAATTGTT AGAGAATAAC TGGCTTTCGG AGAACTCAAT GCAACAGCTG 
TACTGTGATA CATGCGAAAG ATTCTTAGCT GATCGGCTTG TGGAAGGTAA GTGTCCAACA 
GAAGGATGTC ATTATGATTC TGCACGAGGC GATCAGTGTG AAAATTGTGG AAAGCTTTTG 
AATCCTACAG AGTTAATAAA TCCCAGGTGC AAGCAATGTA AAAACATACC ACGAATCCGT 
GATACAAATC ACTTGTTTCT TGAGCTTCCT CTACTGAAGG ATAAATTAGT ACACTATATC 
GAGAGCACAT CTGTAGCTGG GGGCTGGAGC CAAAATGCTA TTCAAACAAC AAATGCATGG 
CTTAAAGAAG GATTAAAACC ACGATGCATT ACCAGGGATC TGAAGTGGGG TGTTCCCGTC 
CCACTTGAAG AATTTAAGGA CAAGGTGTTC TACGTTTGGT TTGATGCTCC TATTGGATAT 
GTCTCCATTA CTTCTTGCTA CACTCCTGAT TGGGAGAAGT GGTGGAAGAA TCCTGAGAAT 
GTTGAGCTGT ATCAGTTTAT GGGAAAAGAC AATGTGCCAT TCCACACTGT GATGTTTCCT 
TCTACGCTTC TTGGAACAGG TGAAAATTGG ACTTTGATGA AGACTATAAG TGTAACTGAA 
TACTTAAATT ATGAAGCAGG AAAGTTTTCC AAGAGTAAAG GTGTAGGAGT GTTTGGTAAT 
GATGCAAAGG ATACAAATAT TCCTGTAGAA GTTTGGAGAT ATTATTTGTT AACCAATAGG 
CCTGAGGTGT CTGACACACT ATTTACATGG TCTGATTTGC AAGCAAAAAG TAACAATGAG 
TTGCTGAATA ATTTGGGCAA CTTTATTAAC CGAGTTCTGA GCTTTCTTGC TAAACCTCCA 
GGTTCAGGAT ATGGTTCTGT TATCCCAGAT GCTCCAGGTG CAGGCTCTCA TCCCCTGACA 
AATAAATTGG CTGGAGAAGT TGGTAAATAT GTGGAGCAAT ACCTGGAAGC CATGGAAAAG 
GTTAAATTAA AGCAGGGATT GAAAACTGCA ATGAGCATTT CTAGCGAGGG AAATGCATAT 
CTGCAAGAAA GCCAATTCTG GAGGCTTTAC AAGGAAGACC AACCTTCTTG TTCTATAGTT 
ATAAAGACTT CTTTGGGTCT AGTATATCTT CTTGCATGTT TGTTAGAACC TTTCATGCCA 
TCTTTCTCTG TTGAGGTCTT CAAGCAGCTT AATATGCTCC CTGAACAAGC TTCACTATGT 
GATGAGAAAG GTGATATGGA TAAGGCAAAA AGGCCTTGGG AGATCCTACC TGCTGGGCAC 
AAGATTGGGA CACCAGAGCC CTTGTTCAAG GAACTGAAAA TAGAAGAAGT GGAGGACTAC 
AGGAAGAAAT TTGCTGGCAG TCAGGCTGAC AGACTTGAGC GGGAAGAAGT TGAAAAGGCA 
TCTAAGCTTG CTGAGGAACT GAAGAAAAAA GCTACAGTTG GTGGGAAGAA GCAGCTGGCC 
AAAAAACCTG CTGGTGAAGC CAAATCAAGG GGCGCTGTTG AACCAGAAAT CTCCATCACA 
AGACTTGATA TTCGTGTTGG CCTAATTAAG AAAGCTCAGA AGCATCCTGA TGCTGATTCC 
CTGTATGTGG AAGAAATCGA CTTGGGTGAA GGAGATTGTA GAACGGTTGT AAGTGGACTT 
GTCAAGTATA TACCTCTTGA GGAGATGCAA AATCGGAAGG TCTGCATCCT TTGCAACCTA 
AAGCCTGCAA ATATGAGGGG CATCAAGTCA GAGGCTATGG TTCTTGCTGC TTCCAGTAGT 
GACCACACCA AGGTCGAATT GGTTGATCCA CCCCAATCTG CCGTTGTTGG AGAGAGAGTG 
ACATTCCCAG GGTTTGAAGG TGAACCTGAC GATGTCCTAA ACCCTAAGAA GAAAGTCTGG 
GAGGCACTAC AGGTGGATCT GCAAACTAAC AGTGACCTGG TAGCATGCTA CAAAGATATT 
CCACTAACCA CATCTGCTGG TGTGTGCAAG GTTGCAACCA TTGGCAATGG TTCCATTAGG 
TAG