LOC105427805 cDNA ORF clone, Pogonomyrmex barbatus(red harvester ant)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105427805
Entrez Gene ID	105427805
Full Name	putative tRNA pseudouridine synthase Pus10
Gene Type	protein-coding
Organism	Pogonomyrmex barbatus(red harvester ant)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011639735.1	XP_011638037.1	putative tRNA pseudouridine synthase Pus10

Pathways from BioSystems
Gene Ontology

The following LOC105427805 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105427805 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo309110	XM_011639735.1 Latest version!	Pogonomyrmex barbatus putative tRNA pseudouridine synthase Pus10 (LOC105427805), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105427805 ( XM_011639735.1 ) cDNA ORF clone, Pogonomyrmex barbatus(red harvester ant) -> XP_011638037.1 Pogonomyrmex barbatus putative tRNA pseudouridine synthase Pus10 (LOC105427805), mRNA.

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CloneID	OPo309110
Clone ID Related Accession (Same CDS sequence)	XM_011639735.1
Accession Version	XM_011639735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-06
Organism	Pogonomyrmex barbatus(red harvester ant)
Product	putative tRNA pseudouridine synthase Pus10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011933671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pogonomyrmex barbatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTCTG GTAGTAAAAT GAATACTATT ACGACGGAGG AAGAGAAAAA AATATTCCAA 
TTTTTAAAAT CCCACGGGTG TTGTTTAAGA TGTTGTTTTC GTTTCATCGG CTACCGCGCG 
CCGAAATATT ATTTCAATCC TTTTGAATTT GCGGTACAGT TGAGTTATGC CAATACAAAA 
GATGAAACCT CTTTGGAAGC ACCTTGCATC ATATGTCTAG GAGTTCTTCA AGATAAGGCA 
CAAGAAAATA TAGTCACAAA AATAGCAAAA AAAGTAAACG AAGAAAATTA TGATTGTACA 
ACATTCACTT GTGCATTAAC TGTTCCTGTA TCAGTGAAGC TGAGAGAATA CATATTGTAT 
GCTTACATGT CTAAGGAAGA AACTCTTGAC AAATCTGCCT TGAGTACTCT TAAAACAAAG 
TTACAAAATG TCAAAGATAT CTGGAAATCA TTTATGATTC CTAAACTCGA AGAAATTATG 
TGCAAACGTG CAGACTTATC GACACCATCG CCTTTTCTAA TCGAGGTCTT TTTAACATAT 
GCTGACAATG AAACGATATT TAAAGAATTA ATGAACAAAT ATAAAGGAGG CAATAATAAA 
CAAAAAAAGA GGAAGTGCAG TGATAACACA TTTAGCAGAA AAGATATTGA AACTATGCTG 
AACGAGATGA CGGATGAGCA ACTTATGCAA CAATTTACAA TTTCACAGAT TGTACCGGAA 
ACATTCGTTC ATATTGATAA TATTTCATGT TCTCACAATA GCATTTTCAT AGGAGGACGC 
TACAGCAAAC TCTCTAGGAA ACTCAGTCAG ACACCATGGT TCATAAACGG TGAGAAGAAA 
GTGGAAACTT CGGTGCAAGA TCTGCTCTGC AATCCCATCG CGGAGATTGT AAAAGCAGAA 
TCGATAAAAT TTTTATCGTC AGGAAGAGAA GACGTCGACG TTAGAAACAT ACATGATGGC 
CGACCGTTTG CTGTCGAGCT ACTGAATCCC CGCATGACGA ATATTACGAG CGAGCTTTTG 
ACGTATCTAA TGGACAATAT CAATCAGTCG ACTAAACAAG TTCAAATAAC ATCGAATTTA 
AAAATTCTTT CAAGGCTCGA TTTGAAGAAA CTCAAGGAAG GTGAGAATGT TAAAACAAAG 
TTCTATCGAG CACTATGTAT CTGCCGGAAT GTCAATGGCG ATTTACCGCG ATTAGAGCAT 
TTGAACAATC TGAAAAACAT TAAAATCATT CAGAAGACAC CGTTGAGAGT GTTGCACAGA 
CGGCCTTTGA GTCCACGGAC GCGCGTTATT TACGAGATGC GGGCGCGTTG GGCGAAACCG 
CATGAGCTGA AGGCACTGCA CACTGCCGCC GAAAGTACTG ACGTGTTTTT CATTCTCGAC 
GTTAAGACGC AAGCGGGTAC GTACGTGAAG GAATTCGTGC ACGGAGATTT TGGCAGAACT 
AATCCGAGTC TATGTAATTT CATCAATGCC GAAGTGGACA TTGTCGCGCT AGATGTCACT 
GGTATTAATT TAATATGGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011638037.1
CDS	325..1848
Translation	MYSGSKMNTITTEEEKKIFQFLKSHGCCLRCCFRFIGYRAPKYYFNPFEFAVQLSYANTKDETSLEAPCIICLGVLQDKAQENIVTKIAKKVNEENYDCTTFTCALTVPVSVKLREYILYAYMSKEETLDKSALSTLKTKLQNVKDIWKSFMIPKLEEIMCKRADLSTPSPFLIEVFLTYADNETIFKELMNKYKGGNNKQKKRKCSDNTFSRKDIETMLNEMTDEQLMQQFTISQIVPETFVHIDNISCSHNSIFIGGRYSKLSRKLSQTPWFINGEKKVETSVQDLLCNPIAEIVKAESIKFLSSGREDVDVRNIHDGRPFAVELLNPRMTNITSELLTYLMDNINQSTKQVQITSNLKILSRLDLKKLKEGENVKTKFYRALCICRNVNGDLPRLEHLNNLKNIKIIQKTPLRVLHRRPLSPRTRVIYEMRARWAKPHELKALHTAAESTDVFFILDVKTQAGTYVKEFVHGDFGRTNPSLCNFINAEVDIVALDVTGINLIWP

Target ORF information:

RefSeq Version	XM_011639735.1
Organism	Pogonomyrmex barbatus(red harvester ant)
Definition	Pogonomyrmex barbatus putative tRNA pseudouridine synthase Pus10 (LOC105427805), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011639735.1

ORF Insert Sequence:

ATGTATTCTG GTAGTAAAAT GAATACTATT ACGACGGAGG AAGAGAAAAA AATATTCCAA 
TTTTTAAAAT CCCACGGGTG TTGTTTAAGA TGTTGTTTTC GTTTCATCGG CTACCGCGCG 
CCGAAATATT ATTTCAATCC TTTTGAATTT GCGGTACAGT TGAGTTATGC CAATACAAAA 
GATGAAACCT CTTTGGAAGC ACCTTGCATC ATATGTCTAG GAGTTCTTCA AGATAAGGCA 
CAAGAAAATA TAGTCACAAA AATAGCAAAA AAAGTAAACG AAGAAAATTA TGATTGTACA 
ACATTCACTT GTGCATTAAC TGTTCCTGTA TCAGTGAAGC TGAGAGAATA CATATTGTAT 
GCTTACATGT CTAAGGAAGA AACTCTTGAC AAATCTGCCT TGAGTACTCT TAAAACAAAG 
TTACAAAATG TCAAAGATAT CTGGAAATCA TTTATGATTC CTAAACTCGA AGAAATTATG 
TGCAAACGTG CAGACTTATC GACACCATCG CCTTTTCTAA TCGAGGTCTT TTTAACATAT 
GCTGACAATG AAACGATATT TAAAGAATTA ATGAACAAAT ATAAAGGAGG CAATAATAAA 
CAAAAAAAGA GGAAGTGCAG TGATAACACA TTTAGCAGAA AAGATATTGA AACTATGCTG 
AACGAGATGA CGGATGAGCA ACTTATGCAA CAATTTACAA TTTCACAGAT TGTACCGGAA 
ACATTCGTTC ATATTGATAA TATTTCATGT TCTCACAATA GCATTTTCAT AGGAGGACGC 
TACAGCAAAC TCTCTAGGAA ACTCAGTCAG ACACCATGGT TCATAAACGG TGAGAAGAAA 
GTGGAAACTT CGGTGCAAGA TCTGCTCTGC AATCCCATCG CGGAGATTGT AAAAGCAGAA 
TCGATAAAAT TTTTATCGTC AGGAAGAGAA GACGTCGACG TTAGAAACAT ACATGATGGC 
CGACCGTTTG CTGTCGAGCT ACTGAATCCC CGCATGACGA ATATTACGAG CGAGCTTTTG 
ACGTATCTAA TGGACAATAT CAATCAGTCG ACTAAACAAG TTCAAATAAC ATCGAATTTA 
AAAATTCTTT CAAGGCTCGA TTTGAAGAAA CTCAAGGAAG GTGAGAATGT TAAAACAAAG 
TTCTATCGAG CACTATGTAT CTGCCGGAAT GTCAATGGCG ATTTACCGCG ATTAGAGCAT 
TTGAACAATC TGAAAAACAT TAAAATCATT CAGAAGACAC CGTTGAGAGT GTTGCACAGA 
CGGCCTTTGA GTCCACGGAC GCGCGTTATT TACGAGATGC GGGCGCGTTG GGCGAAACCG 
CATGAGCTGA AGGCACTGCA CACTGCCGCC GAAAGTACTG ACGTGTTTTT CATTCTCGAC 
GTTAAGACGC AAGCGGGTAC GTACGTGAAG GAATTCGTGC ACGGAGATTT TGGCAGAACT 
AATCCGAGTC TATGTAATTT CATCAATGCC GAAGTGGACA TTGTCGCGCT AGATGTCACT 
GGTATTAATT TAATATGGCC ATGA