DARS2 cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene
Clones
gRNAs

Summary

Gene Symbol	DARS2
Entrez Gene ID	110081647
Full Name	aspartyl-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Pogona vitticeps(central bearded dragon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020798537.1	XP_020654196.1	aspartate--tRNA ligase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following DARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo246431	XM_020798537.1 Latest version!	Pogona vitticeps aspartyl-tRNA synthetase 2, mitochondrial (DARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DARS2 ( XM_020798537.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020654196.1 Pogona vitticeps aspartyl-tRNA synthetase 2, mitochondrial (DARS2), mRNA.

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CloneID	OPo246431
Clone ID Related Accession (Same CDS sequence)	XM_020798537.1
Accession Version	XM_020798537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	aspartate--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018150936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGGC TGCGCTTCCT TCCCGCCGGC GTCTCGTTAC TGCTCCCCAG AGGAGAAGCC 
AAGCGGTGGG GCAGATGCGC TTCTAGCACC TTGGCATCAG ATGTCAATAG TTTTGTCACT 
CGGACGAAAA CATGTGGGGA ATTGCGTTCT ACTCATGTAG GTCAAAACGT CACCCTTTGT 
GGATGGATTC AGTATCAAAG ACAAGAGCAG TTTGTGATTT TGAGAGACTT CCAGGGACTC 
ACCCAGCTTC TTATACCTCA GGACCAGGCT TCTTCCCATT TGAAGAAGAT TTTATATGAC 
GCTCCCGTTG AATCTGTTGT GAGAGTGACA GGGACTGTAG TTCCCAGGCC TCCAGAACAG 
GCAAACCCCA AAATGCCAAC AGGTGACATA GAAGTGAAAG TGGACACAGC AGAGGTCTTA 
AACTTGTGTA AAAAACTACC CTTTGAAGTG AAAGAAGTCT CTAAGAAAAA TGAGGCTTTG 
CGAATGCAGT ATCGCTATTT AGATTTGCGC AGTGTCCAGA TGCAGTACAA CCTACGTCTG 
AGATCCCAGA TGGTGATGAG AATGAGAGAA TTCCTCTGCA ATCATCATGG GTTTGTGGAC 
GTAGAAACTC CAACTCTATT CAAGAAGACT CCAGGAGGTG CAAAGGAATT TGTGGTGCCT 
TCACGAGAAG CTGGAAAGTT CTATTCATTG CCCCAGAGTC CTCAACAATT TAAGCAGCTA 
CTAATGGTTG GAGGTCTGGA CAGGTACTTC CAAGTTGCCC GTTGCTATCG AGATGAAGGT 
TCAAGGCCAG ACAGACAGCC TGAATTCACT CAGATTGATA TTGAGATGTC GTTTGTAGAC 
CAAGCTGGAA TTCAGGCTTT AGTAGAGGGC TTGCTTCAGT ATTCTTGGCC TGAGGAAAGA 
GGGCCCATTG CAATACCATT CCCTTCCATG AACTATGATG AAGCGCTGGC GATGTATGGG 
ACTGATAAGC CAGACACTCG CTTTGGAATG AAGCTTGTTG AAGTCACAGA TATCTTCAGG 
AACGTAGAAG TTGAATTTTT ACAGAAATCA CTCAGTGAGC CACAGAGCAC AGTCAGAGCC 
ATTTGTATAC CCCAGGGCAT GAAATATCTT CAGAATAAAG ATTTGGAGTC ATTAAATGAA 
TTGACAAAAT CCCAGTTTAA CCAGGGACTG TTGCACTTTA TTTTGAGGTC TGATGGTAGC 
ATGAAGTCCC GACTTGATAA ATTCTTGAAT GAAGAGCAAA AACTGGCATT GATCAGAGCA 
GTTCGGGCCA ATGCGGGTGA TGTTGTGTTG CTCTCAGCTG GAGAACATGA GAAAGTGTGC 
TCTGCATTAG GGAAAGTACG CTTGGAGAGT GCAGATCTTC TAGAAGCAGG AGGCCTGCCA 
CTCCGTGACC CATCTGCCTT TCACTTTTTG TGGGTGGTGG ATTTCCCTCT TTTCCTCCAA 
AAAGAAGAAA ATCCTGCAGA ATTAGAGTCA GCTCACCATC CTTTCACTGC CCCTCATCCC 
TCTGATGAGC ATCTTCTCTA CACAGAACCT GCAAAGGTCC GTGGCCAGCA CTATGATCTA 
GTTCTGAATG GTAATGAGAT TGGAGGAGGC TCCATTCGCA TCCACAATGC AAAACAGCAA 
CGCTTCATTC TTGAGGACAT ACTTAAGGAG GATACTGAGC TGCTCTCGCA TCTTCTTGAA 
GCTCTGGACT GTGGAGCACC ACCTCATGGA GGAATTGCAT TAGGTCTTGA CAGACTGATT 
TGTCTCGTAG TTGGAGCTAA AAGTATTAGA GATGTCATTG CTTTCCCCAA ATCCTTCAAA 
GGAAGAGACC TGATGAGCAA TGCTCCAGAT TATGTTACAC CCCAAGAGCT TGAGCCTTAT 
CATGTCCAGG TTGCATGGCC TTTGACACAA GTCAAAGTCA GCTCTAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020654196.1
CDS	157..2067
Translation	MGRLRFLPAGVSLLLPRGEAKRWGRCASSTLASDVNSFVTRTKTCGELRSTHVGQNVTLCGWIQYQRQEQFVILRDFQGLTQLLIPQDQASSHLKKILYDAPVESVVRVTGTVVPRPPEQANPKMPTGDIEVKVDTAEVLNLCKKLPFEVKEVSKKNEALRMQYRYLDLRSVQMQYNLRLRSQMVMRMREFLCNHHGFVDVETPTLFKKTPGGAKEFVVPSREAGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVDQAGIQALVEGLLQYSWPEERGPIAIPFPSMNYDEALAMYGTDKPDTRFGMKLVEVTDIFRNVEVEFLQKSLSEPQSTVRAICIPQGMKYLQNKDLESLNELTKSQFNQGLLHFILRSDGSMKSRLDKFLNEEQKLALIRAVRANAGDVVLLSAGEHEKVCSALGKVRLESADLLEAGGLPLRDPSAFHFLWVVDFPLFLQKEENPAELESAHHPFTAPHPSDEHLLYTEPAKVRGQHYDLVLNGNEIGGGSIRIHNAKQQRFILEDILKEDTELLSHLLEALDCGAPPHGGIALGLDRLICLVVGAKSIRDVIAFPKSFKGRDLMSNAPDYVTPQELEPYHVQVAWPLTQVKVSSS

Target ORF information:

RefSeq Version	XM_020798537.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps aspartyl-tRNA synthetase 2, mitochondrial (DARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020798537.1

ORF Insert Sequence:

ATGGGCCGGC TGCGCTTCCT TCCCGCCGGC GTCTCGTTAC TGCTCCCCAG AGGAGAAGCC 
AAGCGGTGGG GCAGATGCGC TTCTAGCACC TTGGCATCAG ATGTCAATAG TTTTGTCACT 
CGGACGAAAA CATGTGGGGA ATTGCGTTCT ACTCATGTAG GTCAAAACGT CACCCTTTGT 
GGATGGATTC AGTATCAAAG ACAAGAGCAG TTTGTGATTT TGAGAGACTT CCAGGGACTC 
ACCCAGCTTC TTATACCTCA GGACCAGGCT TCTTCCCATT TGAAGAAGAT TTTATATGAC 
GCTCCCGTTG AATCTGTTGT GAGAGTGACA GGGACTGTAG TTCCCAGGCC TCCAGAACAG 
GCAAACCCCA AAATGCCAAC AGGTGACATA GAAGTGAAAG TGGACACAGC AGAGGTCTTA 
AACTTGTGTA AAAAACTACC CTTTGAAGTG AAAGAAGTCT CTAAGAAAAA TGAGGCTTTG 
CGAATGCAGT ATCGCTATTT AGATTTGCGC AGTGTCCAGA TGCAGTACAA CCTACGTCTG 
AGATCCCAGA TGGTGATGAG AATGAGAGAA TTCCTCTGCA ATCATCATGG GTTTGTGGAC 
GTAGAAACTC CAACTCTATT CAAGAAGACT CCAGGAGGTG CAAAGGAATT TGTGGTGCCT 
TCACGAGAAG CTGGAAAGTT CTATTCATTG CCCCAGAGTC CTCAACAATT TAAGCAGCTA 
CTAATGGTTG GAGGTCTGGA CAGGTACTTC CAAGTTGCCC GTTGCTATCG AGATGAAGGT 
TCAAGGCCAG ACAGACAGCC TGAATTCACT CAGATTGATA TTGAGATGTC GTTTGTAGAC 
CAAGCTGGAA TTCAGGCTTT AGTAGAGGGC TTGCTTCAGT ATTCTTGGCC TGAGGAAAGA 
GGGCCCATTG CAATACCATT CCCTTCCATG AACTATGATG AAGCGCTGGC GATGTATGGG 
ACTGATAAGC CAGACACTCG CTTTGGAATG AAGCTTGTTG AAGTCACAGA TATCTTCAGG 
AACGTAGAAG TTGAATTTTT ACAGAAATCA CTCAGTGAGC CACAGAGCAC AGTCAGAGCC 
ATTTGTATAC CCCAGGGCAT GAAATATCTT CAGAATAAAG ATTTGGAGTC ATTAAATGAA 
TTGACAAAAT CCCAGTTTAA CCAGGGACTG TTGCACTTTA TTTTGAGGTC TGATGGTAGC 
ATGAAGTCCC GACTTGATAA ATTCTTGAAT GAAGAGCAAA AACTGGCATT GATCAGAGCA 
GTTCGGGCCA ATGCGGGTGA TGTTGTGTTG CTCTCAGCTG GAGAACATGA GAAAGTGTGC 
TCTGCATTAG GGAAAGTACG CTTGGAGAGT GCAGATCTTC TAGAAGCAGG AGGCCTGCCA 
CTCCGTGACC CATCTGCCTT TCACTTTTTG TGGGTGGTGG ATTTCCCTCT TTTCCTCCAA 
AAAGAAGAAA ATCCTGCAGA ATTAGAGTCA GCTCACCATC CTTTCACTGC CCCTCATCCC 
TCTGATGAGC ATCTTCTCTA CACAGAACCT GCAAAGGTCC GTGGCCAGCA CTATGATCTA 
GTTCTGAATG GTAATGAGAT TGGAGGAGGC TCCATTCGCA TCCACAATGC AAAACAGCAA 
CGCTTCATTC TTGAGGACAT ACTTAAGGAG GATACTGAGC TGCTCTCGCA TCTTCTTGAA 
GCTCTGGACT GTGGAGCACC ACCTCATGGA GGAATTGCAT TAGGTCTTGA CAGACTGATT 
TGTCTCGTAG TTGGAGCTAA AAGTATTAGA GATGTCATTG CTTTCCCCAA ATCCTTCAAA 
GGAAGAGACC TGATGAGCAA TGCTCCAGAT TATGTTACAC CCCAAGAGCT TGAGCCTTAT 
CATGTCCAGG TTGCATGGCC TTTGACACAA GTCAAAGTCA GCTCTAGTTA A