ANO1 cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANO1
Entrez Gene ID	110073504
Full Name	anoctamin 1
Gene Type	protein-coding
Organism	Pogona vitticeps(central bearded dragon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020782784.1	XP_020638443.1	anoctamin-1 isoform X1
XM_020782785.1	XP_020638444.1	anoctamin-1 isoform X2

Pathways from BioSystems
Gene Ontology

The following ANO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo235364	XM_020782784.1 Latest version!	Pogona vitticeps anoctamin 1 (ANO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPo235365	XM_020782785.1 Latest version!	Pogona vitticeps anoctamin 1 (ANO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANO1 ( XM_020782784.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020638443.1 Pogona vitticeps anoctamin 1 (ANO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo235364
Clone ID Related Accession (Same CDS sequence)	XM_020782784.1
Accession Version	XM_020782784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	anoctamin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018150737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGG ATGGCATTGA TCTTCACAGA ATTTCCCGCC AACCATACAC TGGCAGTTCA 
TTGAATGGGG TGGAATTTCT GCCAGAAGAT TTTGAGGATA CTCCGCATGA AACAACAATG 
AGGGTGAATG ATAAATACTC CACGCTACCT GCAGAGGAGA AGGGGATTCA CATTATCAAC 
ATCAGAGCAA TCGAAGATAT AGGATATGTT CGCACAGAAA GCACGATATT AAATTCCTTG 
TCACAAGATC CAGATGCCAA CTGCAAATAT GGCCTTTATT TTAAAGATGG GAAAAGGAAA 
GTGGATTATA TCCTGGTTTA CCACTACAAG AAGTCCTCTT TCAGCAGAAC GCTCACCCGC 
CGCATCAATT TCAGTGACTT GCCACCTCGA AGCATCAAAC AAGAGCAGCC ACTTCCTGGA 
AAAGGAGGGC AGATTAATGT GGATGAAAGT GAACCCCACA TGGACTACCA TGAAGATGAC 
AAGAGATTTC GCAGAGAGGA GTATGAAAGT AACCTTATGC AGGCAGGCCT TGAGTTGGAA 
AGAGATGAAG ATACAAAAAT ACATGGGCTT GGATTTGTAA AAATACACAC ACCATGGAAT 
GTATTATGTC GGGAAGCAGA ATTTATGAAG CTGAAGATGC CCACAAAAAA GATGTATCAA 
ATTCATCAAC CACATGGCCT TTTGAAAAAA ATTAATTCTG TAATTCAAAA AATAACAGAG 
CCTATCCAGC CTAAAGTGGC AGAACACAAG CAACAGACAG TGAAACGCCT CTCTTACCCT 
TTCTCCAGGG AGAAACAACA CTTATTTGAC CTCTCTGACA GAGATTCCTT TTTTGACAGT 
AAAACCAGGA GTACCATAGT TTATGAAATA TTGAAAAGGA CTACATGCAC CAAAGCCAAA 
TACAGCATGG GGCAAGGAGA GGGAAGAAAG AAGGAAACTA CCCTTTTAAA TAAAAGGCGA 
AAATGTGGTA AATATGGGAT CACCAGTCTG CTTGCCAATG GCGTTTACAC TGCTGCATAT 
CCCTTGCATG ATGGTGATTA TGAAGGTGAA CACGTTGAAC CCAATGACAG AAAACTCCTG 
TGTGAGGAAT GGGCAAGCTA TGGAGTCTTC TATAAGTACC AACCAATTGA TCTAGTAAGA 
AAGTACTTTG GTGAAAAGAT TGGACTGTAC TTTGCCTGGC TTGGTGTGTA TACACAAATG 
CTGATTCCAG CTTCCATTGT GGGGATTATC GTGTTTCTGT ATGGCTGTGC AACTGTGGAT 
GAAAATATAC CAAGCATGGA GATGTGTGAC CAAAGAAACA ATATCACCAT GTGCCCCTTA 
TGTGACTCAA CATGTAGCTT CTGGAAATTG AGTTCTGCCT GTGCCACAGC TCGGGCAAGC 
CATCTATTTG ACAATCCAGC AACTGTCTTC TTCTCCGTCT TCATGGCTCT TTGGGCTGCT 
ACATTCATGG AACACTGGAA GAGAAAACAA ATGCGTCTGA ACTATAGATG GGATCTGACT 
GGGTTTGAAG AAGAGGAAGA GGCAGTCAAG GACCATCCAA GGGCTGAGTA TGAAGCTAAA 
GTATTAAAGA AATCACTAAA GAAAGAATAC AAAAATAAAG AGAAGGGCCA GCAATTGCCA 
GAAGAGGCAG CAAACAAATG GCAACAAAGA GTTAAGAGAG TCATGGCTGG GGTGAAATTG 
ACTGAAAAAG AAAAACTGAC ATGGAAAGAT CGTTTTCCTG CCTACTTGAC AAACTTTGTG 
AGCATTATTT TCATGATTGG GCTGACATTT GCTATTGTTT TTGGAGTTAT CATATACCGA 
ATCTCCACTG CAGCAGCTTT AGCCATCAGC TCCTCTCCAG GTGGCCGGTC TAATATCAGA 
GTAACTGTCA CAGCAACTGC AGTGATTATC AATCTGGTGG TTATTATAAT CTTGGATGAA 
GTTTATGGCT GCATTGCCAG ATGGTTGACA CAGATTGAGG TTCCAAAAAC AGACAAGAAT 
TTTGAGGAAC GTCTTATCTT CAAGGCCTTC CTTCTGAAAT TTGTCAATGC CTACACTCCC 
ATTTTCTATG TTGCTTTCTT CAAAGGACGA TTTGTTGGAC GCCCAGGGAA GTATGTCTAC 
ATTTTTCAGT CTTTTCGAAT GGAAGAGTGT GCTCCTGGTG GTTGTCTAAT GGAACTGTGT 
ATACAGCTCA GTATTATTAT GCTTGGCAAA CAGTTGATTC AGAACAACCT CTTTGAAATT 
GGCATCCCGA AAATGAAAAA ATTAATAAGA TATTTGAAGC TGAAACAACA CCATCCTATG 
GACTATGAAG AGCATATGAA GAAAAAGCAA CGTTACGAAG TAGATTATAA CCTGGAGCCT 
TTTGCTGGCC TTACTCCAGA ATACATGGAA ATGATTATCC AGTTCGGGTT CGTTACCCTG 
TTTGTTGCGT CCTTCCCACT GGCTCCCTTG TTCGCATTAC TGAACAACAT CATTGAAATA 
CGTTTGGATG CAAAGAAATT TGTTACTGAG CTTAGAAGGC CAGTCGCAGT TAGAGCAAAA 
GATATAGGAA TATGGTACAA TATCCTCAGA GGTGTAGGAA AGCTTGCTGT GATAATAAAT 
GCATTTGTGA TCTCATTCAC ATCTGACTTC ATTCCACGCC TCGTGTACCT GTATATGTAC 
AGTGAAAGTG GCACCATGCA TGGCTTCGTG AACCATACGC TATCCTATTT TAATGTCAGC 
GATTTTCAAG GAGGAACAGC TCCAAAAGAT CCTATGGAGC TTGGCTACCC AATCCAAATA 
TGCAGATACA AAGACTACAG AGAGCCTCCA TGGTCTGAAA ATAAATATGA CATTGCAAAG 
GATTTCTGGG CTGTACTAGC AGCAAGATTA GCATTTGTAA TAGTGTTCCA GAACTTGGTC 
ATGTTTATGA GTGATTTTGT GGACTGGATT ATACCTGACA TTCCCAAGGA CATCAGTCAG 
CAGATTCATA AGGAAAAAGT TCTGATGGTG GAAGTCTTCA TGAAAGAAGA ACAGGGAAAA 
CTCCAACTAC TAGAAACCTG GATGGACAAG GATAAAAGGA AAGGTGAAAA CTGTAACAAC 
CATAGCCCAA GGTCGTCAAT GAGCCATAGA GGGAGTGTGT CTTCCTGCCA TTCATGCCAT 
GTCGATGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020638443.1
CDS	297..3428
Translation	MDQDGIDLHRISRQPYTGSSLNGVEFLPEDFEDTPHETTMRVNDKYSTLPAEEKGIHIINIRAIEDIGYVRTESTILNSLSQDPDANCKYGLYFKDGKRKVDYILVYHYKKSSFSRTLTRRINFSDLPPRSIKQEQPLPGKGGQINVDESEPHMDYHEDDKRFRREEYESNLMQAGLELERDEDTKIHGLGFVKIHTPWNVLCREAEFMKLKMPTKKMYQIHQPHGLLKKINSVIQKITEPIQPKVAEHKQQTVKRLSYPFSREKQHLFDLSDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRKKETTLLNKRRKCGKYGITSLLANGVYTAAYPLHDGDYEGEHVEPNDRKLLCEEWASYGVFYKYQPIDLVRKYFGEKIGLYFAWLGVYTQMLIPASIVGIIVFLYGCATVDENIPSMEMCDQRNNITMCPLCDSTCSFWKLSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEAKVLKKSLKKEYKNKEKGQQLPEEAANKWQQRVKRVMAGVKLTEKEKLTWKDRFPAYLTNFVSIIFMIGLTFAIVFGVIIYRISTAAALAISSSPGGRSNIRVTVTATAVIINLVVIIILDEVYGCIARWLTQIEVPKTDKNFEERLIFKAFLLKFVNAYTPIFYVAFFKGRFVGRPGKYVYIFQSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKLIRYLKLKQHHPMDYEEHMKKKQRYEVDYNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAVRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSESGTMHGFVNHTLSYFNVSDFQGGTAPKDPMELGYPIQICRYKDYREPPWSENKYDIAKDFWAVLAARLAFVIVFQNLVMFMSDFVDWIIPDIPKDISQQIHKEKVLMVEVFMKEEQGKLQLLETWMDKDKRKGENCNNHSPRSSMSHRGSVSSCHSCHVDV

Target ORF information:

RefSeq Version	XM_020782784.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps anoctamin 1 (ANO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020782784.1

ORF Insert Sequence:

ATGGACCAGG ATGGCATTGA TCTTCACAGA ATTTCCCGCC AACCATACAC TGGCAGTTCA 
TTGAATGGGG TGGAATTTCT GCCAGAAGAT TTTGAGGATA CTCCGCATGA AACAACAATG 
AGGGTGAATG ATAAATACTC CACGCTACCT GCAGAGGAGA AGGGGATTCA CATTATCAAC 
ATCAGAGCAA TCGAAGATAT AGGATATGTT CGCACAGAAA GCACGATATT AAATTCCTTG 
TCACAAGATC CAGATGCCAA CTGCAAATAT GGCCTTTATT TTAAAGATGG GAAAAGGAAA 
GTGGATTATA TCCTGGTTTA CCACTACAAG AAGTCCTCTT TCAGCAGAAC GCTCACCCGC 
CGCATCAATT TCAGTGACTT GCCACCTCGA AGCATCAAAC AAGAGCAGCC ACTTCCTGGA 
AAAGGAGGGC AGATTAATGT GGATGAAAGT GAACCCCACA TGGACTACCA TGAAGATGAC 
AAGAGATTTC GCAGAGAGGA GTATGAAAGT AACCTTATGC AGGCAGGCCT TGAGTTGGAA 
AGAGATGAAG ATACAAAAAT ACATGGGCTT GGATTTGTAA AAATACACAC ACCATGGAAT 
GTATTATGTC GGGAAGCAGA ATTTATGAAG CTGAAGATGC CCACAAAAAA GATGTATCAA 
ATTCATCAAC CACATGGCCT TTTGAAAAAA ATTAATTCTG TAATTCAAAA AATAACAGAG 
CCTATCCAGC CTAAAGTGGC AGAACACAAG CAACAGACAG TGAAACGCCT CTCTTACCCT 
TTCTCCAGGG AGAAACAACA CTTATTTGAC CTCTCTGACA GAGATTCCTT TTTTGACAGT 
AAAACCAGGA GTACCATAGT TTATGAAATA TTGAAAAGGA CTACATGCAC CAAAGCCAAA 
TACAGCATGG GGCAAGGAGA GGGAAGAAAG AAGGAAACTA CCCTTTTAAA TAAAAGGCGA 
AAATGTGGTA AATATGGGAT CACCAGTCTG CTTGCCAATG GCGTTTACAC TGCTGCATAT 
CCCTTGCATG ATGGTGATTA TGAAGGTGAA CACGTTGAAC CCAATGACAG AAAACTCCTG 
TGTGAGGAAT GGGCAAGCTA TGGAGTCTTC TATAAGTACC AACCAATTGA TCTAGTAAGA 
AAGTACTTTG GTGAAAAGAT TGGACTGTAC TTTGCCTGGC TTGGTGTGTA TACACAAATG 
CTGATTCCAG CTTCCATTGT GGGGATTATC GTGTTTCTGT ATGGCTGTGC AACTGTGGAT 
GAAAATATAC CAAGCATGGA GATGTGTGAC CAAAGAAACA ATATCACCAT GTGCCCCTTA 
TGTGACTCAA CATGTAGCTT CTGGAAATTG AGTTCTGCCT GTGCCACAGC TCGGGCAAGC 
CATCTATTTG ACAATCCAGC AACTGTCTTC TTCTCCGTCT TCATGGCTCT TTGGGCTGCT 
ACATTCATGG AACACTGGAA GAGAAAACAA ATGCGTCTGA ACTATAGATG GGATCTGACT 
GGGTTTGAAG AAGAGGAAGA GGCAGTCAAG GACCATCCAA GGGCTGAGTA TGAAGCTAAA 
GTATTAAAGA AATCACTAAA GAAAGAATAC AAAAATAAAG AGAAGGGCCA GCAATTGCCA 
GAAGAGGCAG CAAACAAATG GCAACAAAGA GTTAAGAGAG TCATGGCTGG GGTGAAATTG 
ACTGAAAAAG AAAAACTGAC ATGGAAAGAT CGTTTTCCTG CCTACTTGAC AAACTTTGTG 
AGCATTATTT TCATGATTGG GCTGACATTT GCTATTGTTT TTGGAGTTAT CATATACCGA 
ATCTCCACTG CAGCAGCTTT AGCCATCAGC TCCTCTCCAG GTGGCCGGTC TAATATCAGA 
GTAACTGTCA CAGCAACTGC AGTGATTATC AATCTGGTGG TTATTATAAT CTTGGATGAA 
GTTTATGGCT GCATTGCCAG ATGGTTGACA CAGATTGAGG TTCCAAAAAC AGACAAGAAT 
TTTGAGGAAC GTCTTATCTT CAAGGCCTTC CTTCTGAAAT TTGTCAATGC CTACACTCCC 
ATTTTCTATG TTGCTTTCTT CAAAGGACGA TTTGTTGGAC GCCCAGGGAA GTATGTCTAC 
ATTTTTCAGT CTTTTCGAAT GGAAGAGTGT GCTCCTGGTG GTTGTCTAAT GGAACTGTGT 
ATACAGCTCA GTATTATTAT GCTTGGCAAA CAGTTGATTC AGAACAACCT CTTTGAAATT 
GGCATCCCGA AAATGAAAAA ATTAATAAGA TATTTGAAGC TGAAACAACA CCATCCTATG 
GACTATGAAG AGCATATGAA GAAAAAGCAA CGTTACGAAG TAGATTATAA CCTGGAGCCT 
TTTGCTGGCC TTACTCCAGA ATACATGGAA ATGATTATCC AGTTCGGGTT CGTTACCCTG 
TTTGTTGCGT CCTTCCCACT GGCTCCCTTG TTCGCATTAC TGAACAACAT CATTGAAATA 
CGTTTGGATG CAAAGAAATT TGTTACTGAG CTTAGAAGGC CAGTCGCAGT TAGAGCAAAA 
GATATAGGAA TATGGTACAA TATCCTCAGA GGTGTAGGAA AGCTTGCTGT GATAATAAAT 
GCATTTGTGA TCTCATTCAC ATCTGACTTC ATTCCACGCC TCGTGTACCT GTATATGTAC 
AGTGAAAGTG GCACCATGCA TGGCTTCGTG AACCATACGC TATCCTATTT TAATGTCAGC 
GATTTTCAAG GAGGAACAGC TCCAAAAGAT CCTATGGAGC TTGGCTACCC AATCCAAATA 
TGCAGATACA AAGACTACAG AGAGCCTCCA TGGTCTGAAA ATAAATATGA CATTGCAAAG 
GATTTCTGGG CTGTACTAGC AGCAAGATTA GCATTTGTAA TAGTGTTCCA GAACTTGGTC 
ATGTTTATGA GTGATTTTGT GGACTGGATT ATACCTGACA TTCCCAAGGA CATCAGTCAG 
CAGATTCATA AGGAAAAAGT TCTGATGGTG GAAGTCTTCA TGAAAGAAGA ACAGGGAAAA 
CTCCAACTAC TAGAAACCTG GATGGACAAG GATAAAAGGA AAGGTGAAAA CTGTAACAAC 
CATAGCCCAA GGTCGTCAAT GAGCCATAGA GGGAGTGTGT CTTCCTGCCA TTCATGCCAT 
GTCGATGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANO1 ( XM_020782785.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020638444.1 Pogona vitticeps anoctamin 1 (ANO1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo235365
Clone ID Related Accession (Same CDS sequence)	XM_020782785.1
Accession Version	XM_020782785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	anoctamin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018150737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGG ATGGCATTGA TCTTCACAGA ATTTCCCGCC AACCATACAC TGGCAGTTCA 
TTGAATGGGG TGGAATTTCT GCCAGAAGAT TTTGAGGATA CTCCGCATGA AACAACAATG 
AGGGTGAATG ATAAATACTC CACGCTACCT GCAGAGGAGA AGGGGATTCA CATTATCAAC 
ATCAGAGCAA TCGAAGATAT AGGATATGTT CGCACAGAAA GCACGATATT AAATTCCTTG 
TCACAAGATC CAGATGCCAA CTGCAAATAT GGCCTTTATT TTAAAGATGG GAAAAGGAAA 
GTGGATTATA TCCTGGTTTA CCACTACAAG AAGTCCTCTT TCAGCAGAAC GCTCACCCGC 
CGCATCAATT TCAGTGACTT GCCACCTCGA AGCATCAAAC AAGAGCAGCC ACTTCCTGGA 
AAAGGAGGGC AGATTAATGT GGATGAAAGT GAACCCCACA TGGACTACCA TGAAGATGAC 
AAGAGATTTC GCAGAGAGGA GTATGAAAGT AACCTTATGC AGGCAGGCCT TGAGTTGGAA 
AGAGATGAAG ATACAAAAAT ACATGGGCTT GGATTTGTAA AAATACACAC ACCATGGAAT 
GTATTATGTC GGGAAGCAGA ATTTATGAAG CTGAAGATGC CCACAAAAAA GATGTATCAA 
ATTCATCAAC CACATGGCCT TTTGAAAAAA ATTAATTCTG TAATTCAAAA AATAACAGAG 
CCTATCCAGC CTAAAGTGGC AGAACACAAG CAACAGACAG TGAAACGCCT CTCTTACCCT 
TTCTCCAGGG AGAAACAACA CTTATTTGAC CTCTCTGACA GAGATTCCTT TTTTGACAGT 
AAAACCAGGA GTACCATAGT TTATGAAATA TTGAAAAGGA CTACATGCAC CAAAGCCAAA 
TACAGCATGG GGCAAGGAGA GGGAAGAAAG AAGGAAACTA CCCTTTTAAA TAAAAGGCGA 
AAATGTGGTA AATATGGGAT CACCAGTCTG CTTGCCAATG GCGTTTACAC TGCTGCATAT 
CCCTTGCATG ATGGTGATTA TGAAGGTGAA CACGTTGAAC CCAATGACAG AAAACTCCTG 
TGTGAGGAAT GGGCAAGCTA TGGAGTCTTC TATAAGTACC AACCAATTGA TCTAGTAAGA 
AAGTACTTTG GTGAAAAGAT TGGACTGTAC TTTGCCTGGC TTGGTGTGTA TACACAAATG 
CTGATTCCAG CTTCCATTGT GGGGATTATC GTGTTTCTGT ATGGCTGTGC AACTGTGGAT 
GAAAATATAC CAAGCATGGA GATGTGTGAC CAAAGAAACA ATATCACCAT GTGCCCCTTA 
TGTGACTCAA CATGTAGCTT CTGGAAATTG AGTTCTGCCT GTGCCACAGC TCGGGCAAGC 
CATCTATTTG ACAATCCAGC AACTGTCTTC TTCTCCGTCT TCATGGCTCT TTGGGCTGCT 
ACATTCATGG AACACTGGAA GAGAAAACAA ATGCGTCTGA ACTATAGATG GGATCTGACT 
GGGTTTGAAG AAGAGGAAGA GGCAGTCAAG GACCATCCAA GGGCTGAGTA TGAAGCTAAA 
GTATTAAAGA AATCACTAAA GAAAGAATAC AAAAATAAAG AGACTGAAAA AGAAAAACTG 
ACATGGAAAG ATCGTTTTCC TGCCTACTTG ACAAACTTTG TGAGCATTAT TTTCATGATT 
GGGCTGACAT TTGCTATTGT TTTTGGAGTT ATCATATACC GAATCTCCAC TGCAGCAGCT 
TTAGCCATCA GCTCCTCTCC AGGTGGCCGG TCTAATATCA GAGTAACTGT CACAGCAACT 
GCAGTGATTA TCAATCTGGT GGTTATTATA ATCTTGGATG AAGTTTATGG CTGCATTGCC 
AGATGGTTGA CACAGATTGA GGTTCCAAAA ACAGACAAGA ATTTTGAGGA ACGTCTTATC 
TTCAAGGCCT TCCTTCTGAA ATTTGTCAAT GCCTACACTC CCATTTTCTA TGTTGCTTTC 
TTCAAAGGAC GATTTGTTGG ACGCCCAGGG AAGTATGTCT ACATTTTTCA GTCTTTTCGA 
ATGGAAGAGT GTGCTCCTGG TGGTTGTCTA ATGGAACTGT GTATACAGCT CAGTATTATT 
ATGCTTGGCA AACAGTTGAT TCAGAACAAC CTCTTTGAAA TTGGCATCCC GAAAATGAAA 
AAATTAATAA GATATTTGAA GCTGAAACAA CACCATCCTA TGGACTATGA AGAGCATATG 
AAGAAAAAGC AACGTTACGA AGTAGATTAT AACCTGGAGC CTTTTGCTGG CCTTACTCCA 
GAATACATGG AAATGATTAT CCAGTTCGGG TTCGTTACCC TGTTTGTTGC GTCCTTCCCA 
CTGGCTCCCT TGTTCGCATT ACTGAACAAC ATCATTGAAA TACGTTTGGA TGCAAAGAAA 
TTTGTTACTG AGCTTAGAAG GCCAGTCGCA GTTAGAGCAA AAGATATAGG AATATGGTAC 
AATATCCTCA GAGGTGTAGG AAAGCTTGCT GTGATAATAA ATGCATTTGT GATCTCATTC 
ACATCTGACT TCATTCCACG CCTCGTGTAC CTGTATATGT ACAGTGAAAG TGGCACCATG 
CATGGCTTCG TGAACCATAC GCTATCCTAT TTTAATGTCA GCGATTTTCA AGGAGGAACA 
GCTCCAAAAG ATCCTATGGA GCTTGGCTAC CCAATCCAAA TATGCAGATA CAAAGACTAC 
AGAGAGCCTC CATGGTCTGA AAATAAATAT GACATTGCAA AGGATTTCTG GGCTGTACTA 
GCAGCAAGAT TAGCATTTGT AATAGTGTTC CAGAACTTGG TCATGTTTAT GAGTGATTTT 
GTGGACTGGA TTATACCTGA CATTCCCAAG GACATCAGTC AGCAGATTCA TAAGGAAAAA 
GTTCTGATGG TGGAAGTCTT CATGAAAGAA GAACAGGGAA AACTCCAACT ACTAGAAACC 
TGGATGGACA AGGATAAAAG GAAAGGTGAA AACTGTAACA ACCATAGCCC AAGGTCGTCA 
ATGAGCCATA GAGGGAGTGT GTCTTCCTGC CATTCATGCC ATGTCGATGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020638444.1
CDS	297..3350
Translation	MDQDGIDLHRISRQPYTGSSLNGVEFLPEDFEDTPHETTMRVNDKYSTLPAEEKGIHIINIRAIEDIGYVRTESTILNSLSQDPDANCKYGLYFKDGKRKVDYILVYHYKKSSFSRTLTRRINFSDLPPRSIKQEQPLPGKGGQINVDESEPHMDYHEDDKRFRREEYESNLMQAGLELERDEDTKIHGLGFVKIHTPWNVLCREAEFMKLKMPTKKMYQIHQPHGLLKKINSVIQKITEPIQPKVAEHKQQTVKRLSYPFSREKQHLFDLSDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRKKETTLLNKRRKCGKYGITSLLANGVYTAAYPLHDGDYEGEHVEPNDRKLLCEEWASYGVFYKYQPIDLVRKYFGEKIGLYFAWLGVYTQMLIPASIVGIIVFLYGCATVDENIPSMEMCDQRNNITMCPLCDSTCSFWKLSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEAKVLKKSLKKEYKNKETEKEKLTWKDRFPAYLTNFVSIIFMIGLTFAIVFGVIIYRISTAAALAISSSPGGRSNIRVTVTATAVIINLVVIIILDEVYGCIARWLTQIEVPKTDKNFEERLIFKAFLLKFVNAYTPIFYVAFFKGRFVGRPGKYVYIFQSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKLIRYLKLKQHHPMDYEEHMKKKQRYEVDYNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAVRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSESGTMHGFVNHTLSYFNVSDFQGGTAPKDPMELGYPIQICRYKDYREPPWSENKYDIAKDFWAVLAARLAFVIVFQNLVMFMSDFVDWIIPDIPKDISQQIHKEKVLMVEVFMKEEQGKLQLLETWMDKDKRKGENCNNHSPRSSMSHRGSVSSCHSCHVDV

Target ORF information:

RefSeq Version	XM_020782785.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps anoctamin 1 (ANO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020782785.1

ORF Insert Sequence:

ATGGACCAGG ATGGCATTGA TCTTCACAGA ATTTCCCGCC AACCATACAC TGGCAGTTCA 
TTGAATGGGG TGGAATTTCT GCCAGAAGAT TTTGAGGATA CTCCGCATGA AACAACAATG 
AGGGTGAATG ATAAATACTC CACGCTACCT GCAGAGGAGA AGGGGATTCA CATTATCAAC 
ATCAGAGCAA TCGAAGATAT AGGATATGTT CGCACAGAAA GCACGATATT AAATTCCTTG 
TCACAAGATC CAGATGCCAA CTGCAAATAT GGCCTTTATT TTAAAGATGG GAAAAGGAAA 
GTGGATTATA TCCTGGTTTA CCACTACAAG AAGTCCTCTT TCAGCAGAAC GCTCACCCGC 
CGCATCAATT TCAGTGACTT GCCACCTCGA AGCATCAAAC AAGAGCAGCC ACTTCCTGGA 
AAAGGAGGGC AGATTAATGT GGATGAAAGT GAACCCCACA TGGACTACCA TGAAGATGAC 
AAGAGATTTC GCAGAGAGGA GTATGAAAGT AACCTTATGC AGGCAGGCCT TGAGTTGGAA 
AGAGATGAAG ATACAAAAAT ACATGGGCTT GGATTTGTAA AAATACACAC ACCATGGAAT 
GTATTATGTC GGGAAGCAGA ATTTATGAAG CTGAAGATGC CCACAAAAAA GATGTATCAA 
ATTCATCAAC CACATGGCCT TTTGAAAAAA ATTAATTCTG TAATTCAAAA AATAACAGAG 
CCTATCCAGC CTAAAGTGGC AGAACACAAG CAACAGACAG TGAAACGCCT CTCTTACCCT 
TTCTCCAGGG AGAAACAACA CTTATTTGAC CTCTCTGACA GAGATTCCTT TTTTGACAGT 
AAAACCAGGA GTACCATAGT TTATGAAATA TTGAAAAGGA CTACATGCAC CAAAGCCAAA 
TACAGCATGG GGCAAGGAGA GGGAAGAAAG AAGGAAACTA CCCTTTTAAA TAAAAGGCGA 
AAATGTGGTA AATATGGGAT CACCAGTCTG CTTGCCAATG GCGTTTACAC TGCTGCATAT 
CCCTTGCATG ATGGTGATTA TGAAGGTGAA CACGTTGAAC CCAATGACAG AAAACTCCTG 
TGTGAGGAAT GGGCAAGCTA TGGAGTCTTC TATAAGTACC AACCAATTGA TCTAGTAAGA 
AAGTACTTTG GTGAAAAGAT TGGACTGTAC TTTGCCTGGC TTGGTGTGTA TACACAAATG 
CTGATTCCAG CTTCCATTGT GGGGATTATC GTGTTTCTGT ATGGCTGTGC AACTGTGGAT 
GAAAATATAC CAAGCATGGA GATGTGTGAC CAAAGAAACA ATATCACCAT GTGCCCCTTA 
TGTGACTCAA CATGTAGCTT CTGGAAATTG AGTTCTGCCT GTGCCACAGC TCGGGCAAGC 
CATCTATTTG ACAATCCAGC AACTGTCTTC TTCTCCGTCT TCATGGCTCT TTGGGCTGCT 
ACATTCATGG AACACTGGAA GAGAAAACAA ATGCGTCTGA ACTATAGATG GGATCTGACT 
GGGTTTGAAG AAGAGGAAGA GGCAGTCAAG GACCATCCAA GGGCTGAGTA TGAAGCTAAA 
GTATTAAAGA AATCACTAAA GAAAGAATAC AAAAATAAAG AGACTGAAAA AGAAAAACTG 
ACATGGAAAG ATCGTTTTCC TGCCTACTTG ACAAACTTTG TGAGCATTAT TTTCATGATT 
GGGCTGACAT TTGCTATTGT TTTTGGAGTT ATCATATACC GAATCTCCAC TGCAGCAGCT 
TTAGCCATCA GCTCCTCTCC AGGTGGCCGG TCTAATATCA GAGTAACTGT CACAGCAACT 
GCAGTGATTA TCAATCTGGT GGTTATTATA ATCTTGGATG AAGTTTATGG CTGCATTGCC 
AGATGGTTGA CACAGATTGA GGTTCCAAAA ACAGACAAGA ATTTTGAGGA ACGTCTTATC 
TTCAAGGCCT TCCTTCTGAA ATTTGTCAAT GCCTACACTC CCATTTTCTA TGTTGCTTTC 
TTCAAAGGAC GATTTGTTGG ACGCCCAGGG AAGTATGTCT ACATTTTTCA GTCTTTTCGA 
ATGGAAGAGT GTGCTCCTGG TGGTTGTCTA ATGGAACTGT GTATACAGCT CAGTATTATT 
ATGCTTGGCA AACAGTTGAT TCAGAACAAC CTCTTTGAAA TTGGCATCCC GAAAATGAAA 
AAATTAATAA GATATTTGAA GCTGAAACAA CACCATCCTA TGGACTATGA AGAGCATATG 
AAGAAAAAGC AACGTTACGA AGTAGATTAT AACCTGGAGC CTTTTGCTGG CCTTACTCCA 
GAATACATGG AAATGATTAT CCAGTTCGGG TTCGTTACCC TGTTTGTTGC GTCCTTCCCA 
CTGGCTCCCT TGTTCGCATT ACTGAACAAC ATCATTGAAA TACGTTTGGA TGCAAAGAAA 
TTTGTTACTG AGCTTAGAAG GCCAGTCGCA GTTAGAGCAA AAGATATAGG AATATGGTAC 
AATATCCTCA GAGGTGTAGG AAAGCTTGCT GTGATAATAA ATGCATTTGT GATCTCATTC 
ACATCTGACT TCATTCCACG CCTCGTGTAC CTGTATATGT ACAGTGAAAG TGGCACCATG 
CATGGCTTCG TGAACCATAC GCTATCCTAT TTTAATGTCA GCGATTTTCA AGGAGGAACA 
GCTCCAAAAG ATCCTATGGA GCTTGGCTAC CCAATCCAAA TATGCAGATA CAAAGACTAC 
AGAGAGCCTC CATGGTCTGA AAATAAATAT GACATTGCAA AGGATTTCTG GGCTGTACTA 
GCAGCAAGAT TAGCATTTGT AATAGTGTTC CAGAACTTGG TCATGTTTAT GAGTGATTTT 
GTGGACTGGA TTATACCTGA CATTCCCAAG GACATCAGTC AGCAGATTCA TAAGGAAAAA 
GTTCTGATGG TGGAAGTCTT CATGAAAGAA GAACAGGGAA AACTCCAACT ACTAGAAACC 
TGGATGGACA AGGATAAAAG GAAAGGTGAA AACTGTAACA ACCATAGCCC AAGGTCGTCA 
ATGAGCCATA GAGGGAGTGT GTCTTCCTGC CATTCATGCC ATGTCGATGT GTAG