col8a1 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	col8a1
Entrez Gene ID	103479469
Full Name	collagen type VIII alpha 1 chain
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008433903.1	XP_008432125.1	collagen alpha-1(VIII) chain isoform X1
XM_008433912.1	XP_008432134.1	collagen alpha-1(VIII) chain isoform X2

Pathways from BioSystems
Gene Ontology

The following col8a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col8a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo185421	XM_008433903.1 Latest version!	Poecilia reticulata collagen type VIII alpha 1 (col8a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OPo185422	XM_008433912.1 Latest version!	Poecilia reticulata collagen type VIII alpha 1 (col8a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col8a1 ( XM_008433903.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008432125.1 Poecilia reticulata collagen type VIII alpha 1 (col8a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo185421
Clone ID Related Accession (Same CDS sequence)	XM_008433903.1
Accession Version	XM_008433903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	collagen alpha-1(VIII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024332.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGCAT GTGTGCAGAA AGTTGAAGCC AAACTGACAG ATGAATGCGA CGGGGGCGAG 
AGCAGCAGTT TGGCTTTGGT CATGCCTCCC CTTCTTAGGC CCCTCGCCAC TGTTCTGCTC 
CAGCTTGTCG TCTTCATATC TGTGACTGGT GGTGCATACT ACGGACACAC TCAACACCCC 
CAGCCGTACC AGCCAATGCA CCACCTTCAG CACATTAACA TGGGCAAGGG AGGTTTTCCT 
CAGCAACAGT ACCTTGGCAA AGAAGTGCCC TACATGCAGT TTCCACACTA CAGGAAGGAG 
CTGCCCCAGA CCCCCCTGCT CAAAGGCAAA GAAAATCCTC GGAAAGGGGG AAACTACAAT 
GCTGGCAAAG ACAACGGTCT AACAATTCCT AGTGCAGTGG AAGGAGCTGC CCTGAGAGAA 
CAAGGCCCAG CTGGACCACC TGGACCAGAG GGACCTCCAG GGCCTCCAGG AGATGGTTTG 
CCTGGTCCTG AAGGCCAACT GGGACCTCCA GGTCCACCAG GACTGCCTGG AATAGGGAAA 
CTAGGCTTGC CTGGAGTGCC AGGAAAACCA GGTGTAATTG GTGAGCCTGG GCCTCAGGGA 
AAAATGGGAC CCAGAGGTGA AGAAGGCCCC ACTGGTCAAC CAGGTCCTCA GGGACCCTCG 
GGCCCACCTG GGCTTCCTGG AATAGGTAAA CCAGGGATGC AGGGATTACC AGGACAACCA 
GGTGGCAAAG GAGAACCAGG TCACAAGGGC CTGCCAGGAC TGCCGGGATT ACCGGGACCA 
AAAGGAGACA AAGGAATGGG CCTTCCAGGA CAGCCTGGTC ACAAAGGACT TCCTGGGCCT 
CCTGGACCGG CTGGCCCAAG AGGGCTGCCA GGTTTTGGAA AACCAGGGTT GAATGGTTTG 
CCAGGTCCAC AAGGACCACC AGGACATAAA GGACATTCTG GGGAATCAGG GCTTCCTGGG 
CCAGCTGGGA AAGGAGGACC ACCTGGCCCT CCAGGTCTAC CTGGTCAAGG AAAGCCAGGT 
CAAAATGGTC TGCCTGGAGA ACCAGGAAGG ACAGGTGAAA AGGGCCCTAC AGGACCACCA 
GGATTGCCAG GAAAACCAGG ACTTCCAGGA TTTGGTAAAC AAGGACTTTC AGGACCAAAA 
GGTGATAAAG GAATGAATGG ACCACCTGGG CCTCCAGGAC TCAAAGGAGA TAAGGGCCAT 
GGCGGATTAC CTGGAATGCT TGGACCTCCT GGACCAAGTG GTCCATCAGG TCCTCCAGGA 
CCTATTGGAC GACCTGGAAG CGTAGGTGCA CCTGGTTCCA AAGGAGATTG TGGAGACGGA 
GGACCTAAAG GGCTTGATGG GGCCAAAGGT GACCTTGGTC CTCCAGGGCT TCCTGGTAAG 
ACTGGTCTAA CTGGGGACCT TGGGGAACCA GGTCCTAGAG GTCCACCTGG ACCAAGTGGT 
CCTAAAGGTG CTGATGGGTA TAAAGGTCTA CCTGGTATAC CTGGTCCCCC GGGAACTCCT 
GGTTTAAGAG GAGTGGCTGG ATTACCTGGT GAAAAGGGTA TTCAAGGATC TCAAGGAATC 
CCTGGAATAG ACGGTACAGC TGGACCAATG GGATCTCCAG GTCCCACAGG ACCTAAAGGA 
GAAAATGGAC TTCCTGGTCC ACCAGGCACT GCAGTCATTG GGAGTCCAGG CCTGCCTGGT 
CCTGCAGGAC CCCAAGGAAG AGAGGGATTG TCTGGACTCC CAGGACAGCC AGGTCAACCT 
GGTCCACCTG GACCACCAGG ACCACCTGGT GAACCTGTTT TCTCTCCAGA AATGGCTGGG 
GTTCTCTCTG AGATGGGACC AGGACTAGAT GGTATTAAAG CCGGAAGTTA TGGCAAAAAG 
AGCAAATACG GTGGAGGTGG TGCTGAAGTA ATGGGTTCCA GTGGACTTGA AATGCCAGCA 
TTTACGGCTG TAGCGTCCAC TCCCTTCCCA CCTGTGGGGA GTCCGGTTGT TTTTGATAAG 
CTGTTGTACA ACGGCCGCCA AAACTACAAT CCTCAGACAG GAATCTTCAC CTGTGACATC 
CCCGGCATTT ACTACTTTGC CTACCACATT CATTGCAAGG GAGAGAACGT GTGGGTGGCT 
TTGATGAGAA ACAATGAGCC TGTGATGTAT ACATATGATG AATACAAAAA AGGTTCCTTA 
GACCAAGCCT CTGGAAGTGC AGTCCTGCCT CTCCAACCCG GGGACACTGT GTATATTCAA 
ATGCCATCAG ATCAAGCCTC AGGACTTTAT GCTGGAGAGT ATGTGCAGTC CTCCTTTACT 
GGGTATTTGC TCTATCCAAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008432125.1
CDS	11..2314
Translation	MRACVQKVEAKLTDECDGGESSSLALVMPPLLRPLATVLLQLVVFISVTGGAYYGHTQHPQPYQPMHHLQHINMGKGGFPQQQYLGKEVPYMQFPHYRKELPQTPLLKGKENPRKGGNYNAGKDNGLTIPSAVEGAALREQGPAGPPGPEGPPGPPGDGLPGPEGQLGPPGPPGLPGIGKLGLPGVPGKPGVIGEPGPQGKMGPRGEEGPTGQPGPQGPSGPPGLPGIGKPGMQGLPGQPGGKGEPGHKGLPGLPGLPGPKGDKGMGLPGQPGHKGLPGPPGPAGPRGLPGFGKPGLNGLPGPQGPPGHKGHSGESGLPGPAGKGGPPGPPGLPGQGKPGQNGLPGEPGRTGEKGPTGPPGLPGKPGLPGFGKQGLSGPKGDKGMNGPPGPPGLKGDKGHGGLPGMLGPPGPSGPSGPPGPIGRPGSVGAPGSKGDCGDGGPKGLDGAKGDLGPPGLPGKTGLTGDLGEPGPRGPPGPSGPKGADGYKGLPGIPGPPGTPGLRGVAGLPGEKGIQGSQGIPGIDGTAGPMGSPGPTGPKGENGLPGPPGTAVIGSPGLPGPAGPQGREGLSGLPGQPGQPGPPGPPGPPGEPVFSPEMAGVLSEMGPGLDGIKAGSYGKKSKYGGGGAEVMGSSGLEMPAFTAVASTPFPPVGSPVVFDKLLYNGRQNYNPQTGIFTCDIPGIYYFAYHIHCKGENVWVALMRNNEPVMYTYDEYKKGSLDQASGSAVLPLQPGDTVYIQMPSDQASGLYAGEYVQSSFTGYLLYPM

Target ORF information:

RefSeq Version	XM_008433903.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata collagen type VIII alpha 1 (col8a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008433903.1

ORF Insert Sequence:

ATGCGTGCAT GTGTGCAGAA AGTTGAAGCC AAACTGACAG ATGAATGCGA CGGGGGCGAG 
AGCAGCAGTT TGGCTTTGGT CATGCCTCCC CTTCTTAGGC CCCTCGCCAC TGTTCTGCTC 
CAGCTTGTCG TCTTCATATC TGTGACTGGT GGTGCATACT ACGGACACAC TCAACACCCC 
CAGCCGTACC AGCCAATGCA CCACCTTCAG CACATTAACA TGGGCAAGGG AGGTTTTCCT 
CAGCAACAGT ACCTTGGCAA AGAAGTGCCC TACATGCAGT TTCCACACTA CAGGAAGGAG 
CTGCCCCAGA CCCCCCTGCT CAAAGGCAAA GAAAATCCTC GGAAAGGGGG AAACTACAAT 
GCTGGCAAAG ACAACGGTCT AACAATTCCT AGTGCAGTGG AAGGAGCTGC CCTGAGAGAA 
CAAGGCCCAG CTGGACCACC TGGACCAGAG GGACCTCCAG GGCCTCCAGG AGATGGTTTG 
CCTGGTCCTG AAGGCCAACT GGGACCTCCA GGTCCACCAG GACTGCCTGG AATAGGGAAA 
CTAGGCTTGC CTGGAGTGCC AGGAAAACCA GGTGTAATTG GTGAGCCTGG GCCTCAGGGA 
AAAATGGGAC CCAGAGGTGA AGAAGGCCCC ACTGGTCAAC CAGGTCCTCA GGGACCCTCG 
GGCCCACCTG GGCTTCCTGG AATAGGTAAA CCAGGGATGC AGGGATTACC AGGACAACCA 
GGTGGCAAAG GAGAACCAGG TCACAAGGGC CTGCCAGGAC TGCCGGGATT ACCGGGACCA 
AAAGGAGACA AAGGAATGGG CCTTCCAGGA CAGCCTGGTC ACAAAGGACT TCCTGGGCCT 
CCTGGACCGG CTGGCCCAAG AGGGCTGCCA GGTTTTGGAA AACCAGGGTT GAATGGTTTG 
CCAGGTCCAC AAGGACCACC AGGACATAAA GGACATTCTG GGGAATCAGG GCTTCCTGGG 
CCAGCTGGGA AAGGAGGACC ACCTGGCCCT CCAGGTCTAC CTGGTCAAGG AAAGCCAGGT 
CAAAATGGTC TGCCTGGAGA ACCAGGAAGG ACAGGTGAAA AGGGCCCTAC AGGACCACCA 
GGATTGCCAG GAAAACCAGG ACTTCCAGGA TTTGGTAAAC AAGGACTTTC AGGACCAAAA 
GGTGATAAAG GAATGAATGG ACCACCTGGG CCTCCAGGAC TCAAAGGAGA TAAGGGCCAT 
GGCGGATTAC CTGGAATGCT TGGACCTCCT GGACCAAGTG GTCCATCAGG TCCTCCAGGA 
CCTATTGGAC GACCTGGAAG CGTAGGTGCA CCTGGTTCCA AAGGAGATTG TGGAGACGGA 
GGACCTAAAG GGCTTGATGG GGCCAAAGGT GACCTTGGTC CTCCAGGGCT TCCTGGTAAG 
ACTGGTCTAA CTGGGGACCT TGGGGAACCA GGTCCTAGAG GTCCACCTGG ACCAAGTGGT 
CCTAAAGGTG CTGATGGGTA TAAAGGTCTA CCTGGTATAC CTGGTCCCCC GGGAACTCCT 
GGTTTAAGAG GAGTGGCTGG ATTACCTGGT GAAAAGGGTA TTCAAGGATC TCAAGGAATC 
CCTGGAATAG ACGGTACAGC TGGACCAATG GGATCTCCAG GTCCCACAGG ACCTAAAGGA 
GAAAATGGAC TTCCTGGTCC ACCAGGCACT GCAGTCATTG GGAGTCCAGG CCTGCCTGGT 
CCTGCAGGAC CCCAAGGAAG AGAGGGATTG TCTGGACTCC CAGGACAGCC AGGTCAACCT 
GGTCCACCTG GACCACCAGG ACCACCTGGT GAACCTGTTT TCTCTCCAGA AATGGCTGGG 
GTTCTCTCTG AGATGGGACC AGGACTAGAT GGTATTAAAG CCGGAAGTTA TGGCAAAAAG 
AGCAAATACG GTGGAGGTGG TGCTGAAGTA ATGGGTTCCA GTGGACTTGA AATGCCAGCA 
TTTACGGCTG TAGCGTCCAC TCCCTTCCCA CCTGTGGGGA GTCCGGTTGT TTTTGATAAG 
CTGTTGTACA ACGGCCGCCA AAACTACAAT CCTCAGACAG GAATCTTCAC CTGTGACATC 
CCCGGCATTT ACTACTTTGC CTACCACATT CATTGCAAGG GAGAGAACGT GTGGGTGGCT 
TTGATGAGAA ACAATGAGCC TGTGATGTAT ACATATGATG AATACAAAAA AGGTTCCTTA 
GACCAAGCCT CTGGAAGTGC AGTCCTGCCT CTCCAACCCG GGGACACTGT GTATATTCAA 
ATGCCATCAG ATCAAGCCTC AGGACTTTAT GCTGGAGAGT ATGTGCAGTC CTCCTTTACT 
GGGTATTTGC TCTATCCAAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col8a1 ( XM_008433912.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008432134.1 Poecilia reticulata collagen type VIII alpha 1 (col8a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo185422
Clone ID Related Accession (Same CDS sequence)	XM_008433912.1
Accession Version	XM_008433912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	collagen alpha-1(VIII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024332.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCC TTCTTAGGCC CCTCGCCACT GTTCTGCTCC AGCTTGTCGT CTTCATATCT 
GTGACTGGTG GTGCATACTA CGGACACACT CAACACCCCC AGCCGTACCA GCCAATGCAC 
CACCTTCAGC ACATTAACAT GGGCAAGGGA GGTTTTCCTC AGCAACAGTA CCTTGGCAAA 
GAAGTGCCCT ACATGCAGTT TCCACACTAC AGGAAGGAGC TGCCCCAGAC CCCCCTGCTC 
AAAGGCAAAG AAAATCCTCG GAAAGGGGGA AACTACAATG CTGGCAAAGA CAACGGTCTA 
ACAATTCCTA GTGCAGTGGA AGGAGCTGCC CTGAGAGAAC AAGGCCCAGC TGGACCACCT 
GGACCAGAGG GACCTCCAGG GCCTCCAGGA GATGGTTTGC CTGGTCCTGA AGGCCAACTG 
GGACCTCCAG GTCCACCAGG ACTGCCTGGA ATAGGGAAAC TAGGCTTGCC TGGAGTGCCA 
GGAAAACCAG GTGTAATTGG TGAGCCTGGG CCTCAGGGAA AAATGGGACC CAGAGGTGAA 
GAAGGCCCCA CTGGTCAACC AGGTCCTCAG GGACCCTCGG GCCCACCTGG GCTTCCTGGA 
ATAGGTAAAC CAGGGATGCA GGGATTACCA GGACAACCAG GTGGCAAAGG AGAACCAGGT 
CACAAGGGCC TGCCAGGACT GCCGGGATTA CCGGGACCAA AAGGAGACAA AGGAATGGGC 
CTTCCAGGAC AGCCTGGTCA CAAAGGACTT CCTGGGCCTC CTGGACCGGC TGGCCCAAGA 
GGGCTGCCAG GTTTTGGAAA ACCAGGGTTG AATGGTTTGC CAGGTCCACA AGGACCACCA 
GGACATAAAG GACATTCTGG GGAATCAGGG CTTCCTGGGC CAGCTGGGAA AGGAGGACCA 
CCTGGCCCTC CAGGTCTACC TGGTCAAGGA AAGCCAGGTC AAAATGGTCT GCCTGGAGAA 
CCAGGAAGGA CAGGTGAAAA GGGCCCTACA GGACCACCAG GATTGCCAGG AAAACCAGGA 
CTTCCAGGAT TTGGTAAACA AGGACTTTCA GGACCAAAAG GTGATAAAGG AATGAATGGA 
CCACCTGGGC CTCCAGGACT CAAAGGAGAT AAGGGCCATG GCGGATTACC TGGAATGCTT 
GGACCTCCTG GACCAAGTGG TCCATCAGGT CCTCCAGGAC CTATTGGACG ACCTGGAAGC 
GTAGGTGCAC CTGGTTCCAA AGGAGATTGT GGAGACGGAG GACCTAAAGG GCTTGATGGG 
GCCAAAGGTG ACCTTGGTCC TCCAGGGCTT CCTGGTAAGA CTGGTCTAAC TGGGGACCTT 
GGGGAACCAG GTCCTAGAGG TCCACCTGGA CCAAGTGGTC CTAAAGGTGC TGATGGGTAT 
AAAGGTCTAC CTGGTATACC TGGTCCCCCG GGAACTCCTG GTTTAAGAGG AGTGGCTGGA 
TTACCTGGTG AAAAGGGTAT TCAAGGATCT CAAGGAATCC CTGGAATAGA CGGTACAGCT 
GGACCAATGG GATCTCCAGG TCCCACAGGA CCTAAAGGAG AAAATGGACT TCCTGGTCCA 
CCAGGCACTG CAGTCATTGG GAGTCCAGGC CTGCCTGGTC CTGCAGGACC CCAAGGAAGA 
GAGGGATTGT CTGGACTCCC AGGACAGCCA GGTCAACCTG GTCCACCTGG ACCACCAGGA 
CCACCTGGTG AACCTGTTTT CTCTCCAGAA ATGGCTGGGG TTCTCTCTGA GATGGGACCA 
GGACTAGATG GTATTAAAGC CGGAAGTTAT GGCAAAAAGA GCAAATACGG TGGAGGTGGT 
GCTGAAGTAA TGGGTTCCAG TGGACTTGAA ATGCCAGCAT TTACGGCTGT AGCGTCCACT 
CCCTTCCCAC CTGTGGGGAG TCCGGTTGTT TTTGATAAGC TGTTGTACAA CGGCCGCCAA 
AACTACAATC CTCAGACAGG AATCTTCACC TGTGACATCC CCGGCATTTA CTACTTTGCC 
TACCACATTC ATTGCAAGGG AGAGAACGTG TGGGTGGCTT TGATGAGAAA CAATGAGCCT 
GTGATGTATA CATATGATGA ATACAAAAAA GGTTCCTTAG ACCAAGCCTC TGGAAGTGCA 
GTCCTGCCTC TCCAACCCGG GGACACTGTG TATATTCAAA TGCCATCAGA TCAAGCCTCA 
GGACTTTATG CTGGAGAGTA TGTGCAGTCC TCCTTTACTG GGTATTTGCT CTATCCAATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008432134.1
CDS	90..2312
Translation	MPPLLRPLATVLLQLVVFISVTGGAYYGHTQHPQPYQPMHHLQHINMGKGGFPQQQYLGKEVPYMQFPHYRKELPQTPLLKGKENPRKGGNYNAGKDNGLTIPSAVEGAALREQGPAGPPGPEGPPGPPGDGLPGPEGQLGPPGPPGLPGIGKLGLPGVPGKPGVIGEPGPQGKMGPRGEEGPTGQPGPQGPSGPPGLPGIGKPGMQGLPGQPGGKGEPGHKGLPGLPGLPGPKGDKGMGLPGQPGHKGLPGPPGPAGPRGLPGFGKPGLNGLPGPQGPPGHKGHSGESGLPGPAGKGGPPGPPGLPGQGKPGQNGLPGEPGRTGEKGPTGPPGLPGKPGLPGFGKQGLSGPKGDKGMNGPPGPPGLKGDKGHGGLPGMLGPPGPSGPSGPPGPIGRPGSVGAPGSKGDCGDGGPKGLDGAKGDLGPPGLPGKTGLTGDLGEPGPRGPPGPSGPKGADGYKGLPGIPGPPGTPGLRGVAGLPGEKGIQGSQGIPGIDGTAGPMGSPGPTGPKGENGLPGPPGTAVIGSPGLPGPAGPQGREGLSGLPGQPGQPGPPGPPGPPGEPVFSPEMAGVLSEMGPGLDGIKAGSYGKKSKYGGGGAEVMGSSGLEMPAFTAVASTPFPPVGSPVVFDKLLYNGRQNYNPQTGIFTCDIPGIYYFAYHIHCKGENVWVALMRNNEPVMYTYDEYKKGSLDQASGSAVLPLQPGDTVYIQMPSDQASGLYAGEYVQSSFTGYLLYPM

Target ORF information:

RefSeq Version	XM_008433912.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata collagen type VIII alpha 1 (col8a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008433912.1

ORF Insert Sequence:

ATGCCTCCCC TTCTTAGGCC CCTCGCCACT GTTCTGCTCC AGCTTGTCGT CTTCATATCT 
GTGACTGGTG GTGCATACTA CGGACACACT CAACACCCCC AGCCGTACCA GCCAATGCAC 
CACCTTCAGC ACATTAACAT GGGCAAGGGA GGTTTTCCTC AGCAACAGTA CCTTGGCAAA 
GAAGTGCCCT ACATGCAGTT TCCACACTAC AGGAAGGAGC TGCCCCAGAC CCCCCTGCTC 
AAAGGCAAAG AAAATCCTCG GAAAGGGGGA AACTACAATG CTGGCAAAGA CAACGGTCTA 
ACAATTCCTA GTGCAGTGGA AGGAGCTGCC CTGAGAGAAC AAGGCCCAGC TGGACCACCT 
GGACCAGAGG GACCTCCAGG GCCTCCAGGA GATGGTTTGC CTGGTCCTGA AGGCCAACTG 
GGACCTCCAG GTCCACCAGG ACTGCCTGGA ATAGGGAAAC TAGGCTTGCC TGGAGTGCCA 
GGAAAACCAG GTGTAATTGG TGAGCCTGGG CCTCAGGGAA AAATGGGACC CAGAGGTGAA 
GAAGGCCCCA CTGGTCAACC AGGTCCTCAG GGACCCTCGG GCCCACCTGG GCTTCCTGGA 
ATAGGTAAAC CAGGGATGCA GGGATTACCA GGACAACCAG GTGGCAAAGG AGAACCAGGT 
CACAAGGGCC TGCCAGGACT GCCGGGATTA CCGGGACCAA AAGGAGACAA AGGAATGGGC 
CTTCCAGGAC AGCCTGGTCA CAAAGGACTT CCTGGGCCTC CTGGACCGGC TGGCCCAAGA 
GGGCTGCCAG GTTTTGGAAA ACCAGGGTTG AATGGTTTGC CAGGTCCACA AGGACCACCA 
GGACATAAAG GACATTCTGG GGAATCAGGG CTTCCTGGGC CAGCTGGGAA AGGAGGACCA 
CCTGGCCCTC CAGGTCTACC TGGTCAAGGA AAGCCAGGTC AAAATGGTCT GCCTGGAGAA 
CCAGGAAGGA CAGGTGAAAA GGGCCCTACA GGACCACCAG GATTGCCAGG AAAACCAGGA 
CTTCCAGGAT TTGGTAAACA AGGACTTTCA GGACCAAAAG GTGATAAAGG AATGAATGGA 
CCACCTGGGC CTCCAGGACT CAAAGGAGAT AAGGGCCATG GCGGATTACC TGGAATGCTT 
GGACCTCCTG GACCAAGTGG TCCATCAGGT CCTCCAGGAC CTATTGGACG ACCTGGAAGC 
GTAGGTGCAC CTGGTTCCAA AGGAGATTGT GGAGACGGAG GACCTAAAGG GCTTGATGGG 
GCCAAAGGTG ACCTTGGTCC TCCAGGGCTT CCTGGTAAGA CTGGTCTAAC TGGGGACCTT 
GGGGAACCAG GTCCTAGAGG TCCACCTGGA CCAAGTGGTC CTAAAGGTGC TGATGGGTAT 
AAAGGTCTAC CTGGTATACC TGGTCCCCCG GGAACTCCTG GTTTAAGAGG AGTGGCTGGA 
TTACCTGGTG AAAAGGGTAT TCAAGGATCT CAAGGAATCC CTGGAATAGA CGGTACAGCT 
GGACCAATGG GATCTCCAGG TCCCACAGGA CCTAAAGGAG AAAATGGACT TCCTGGTCCA 
CCAGGCACTG CAGTCATTGG GAGTCCAGGC CTGCCTGGTC CTGCAGGACC CCAAGGAAGA 
GAGGGATTGT CTGGACTCCC AGGACAGCCA GGTCAACCTG GTCCACCTGG ACCACCAGGA 
CCACCTGGTG AACCTGTTTT CTCTCCAGAA ATGGCTGGGG TTCTCTCTGA GATGGGACCA 
GGACTAGATG GTATTAAAGC CGGAAGTTAT GGCAAAAAGA GCAAATACGG TGGAGGTGGT 
GCTGAAGTAA TGGGTTCCAG TGGACTTGAA ATGCCAGCAT TTACGGCTGT AGCGTCCACT 
CCCTTCCCAC CTGTGGGGAG TCCGGTTGTT TTTGATAAGC TGTTGTACAA CGGCCGCCAA 
AACTACAATC CTCAGACAGG AATCTTCACC TGTGACATCC CCGGCATTTA CTACTTTGCC 
TACCACATTC ATTGCAAGGG AGAGAACGTG TGGGTGGCTT TGATGAGAAA CAATGAGCCT 
GTGATGTATA CATATGATGA ATACAAAAAA GGTTCCTTAG ACCAAGCCTC TGGAAGTGCA 
GTCCTGCCTC TCCAACCCGG GGACACTGTG TATATTCAAA TGCCATCAGA TCAAGCCTCA 
GGACTTTATG CTGGAGAGTA TGTGCAGTCC TCCTTTACTG GGTATTTGCT CTATCCAATG 
TAA