agl cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	agl
Entrez Gene ID	103479203
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008433499.2	XP_008431721.1	glycogen debranching enzyme isoform X1
XM_017309975.1	XP_017165464.1	glycogen debranching enzyme isoform X3
XM_008433503.2	XP_008431725.1	glycogen debranching enzyme isoform X4
XM_017309976.1	XP_017165465.1	glycogen debranching enzyme isoform X5
XM_008433500.2	XP_008431722.1	glycogen debranching enzyme isoform X2
XM_008433502.2	XP_008431724.1	glycogen debranching enzyme isoform X3
XM_008433504.1	XP_008431726.1	glycogen debranching enzyme isoform X6

Pathways from BioSystems
Gene Ontology

The following agl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the agl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPo179982	XM_008433504.1 Latest version!	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPo179977	XM_008433499.2 Latest version!	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPo179978	XM_008433500.2 Latest version!	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPo179979	XM_017309975.1 Latest version!	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPo179979	XM_008433502.2 Latest version!	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPo179980	XM_008433503.2 Latest version!	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPo179981	XM_017309976.1 Latest version!	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

agl ( XM_008433504.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008431726.1 Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OPo179982
Clone ID Related Accession (Same CDS sequence)	XM_008433504.1
Accession Version	XM_008433504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	glycogen debranching enzyme isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTAC TTACAGGTTT CTTCTTCAGT TTGAGACTCC TCATTGTAAT CTGTCTAATG 
CCCGTCTCTT TGGAGACCAG GGTTCCCTCC AAGTTCAGGA GAACTTCCAT GATGCTGCCT 
CAGCACCTAA AGCAAATCAG AGTACTCATG CTTAACGAAA AGGAGAATTT AGAGAGAACT 
TTGTTCAGAC TTGAACAAGG TTTTGAGCTC CAGTTCCGCC TGGGTCCAAG CCTTCAAGGA 
AGGAGAGTTA TAGTTCACAC CAACTACCCA CTTGATGGAC AGAAGTTCAT TCGAAACAAC 
TTCCGTGTTT TAGCCTGGAA CTACCCAACA GGAAGGGAGG ATGACTCTGA TAAGTACTGC 
TCCCTAGAGC TCAAGATTGC TGGATCCTAC CAGTACTACT TTGGATATGC AGATGTGGAG 
AGGTTAGGAG GAGGCTACGT CGTCGTTGAT CCTATTCTTC GTGTTGGAGC AGACAACCAT 
GTCCTCCTGC TGGACTGTGT CACCATCCAG ACATACTTGT CCAAATGTCT GGGACGCCTT 
GATGACTGGC CAGACAGACT GCGAGTAGCC AAGGAGTCAG GTTACAACAT GATCCACTTC 
ACTCCTCTGC AGACTTTGGG AGAGTCTAGG TCCTGTTACT CCTTAGCTGA CCAGCTGACC 
TTTAGCCTGG AATTCAGCCC AGATGGCAAG AGTTACACCT GGGAAGATGT GGGAAGGCTA 
GTGGAAAAAC TGAGGACGGA ATGGAATATG GTATCAATCA CTGATGTAGT GTATAATCAC 
ACTGCTGCAA ACAGTGTGTG GATAAAGGAA CATCCAGAGT GTGGCTACAA CCTGATGAAC 
TCTCCTCACC TGCGTCCTGC CTGGGTCTTA GACAGAGCTA TCTGGCATTT AACCACTAGG 
GTAGCAGATG GACGGTACAA AGAGAAGGGA CTTCCTTCTG ACATTACCAA CGAGAGCCAC 
CTAAATGCTC TCCGCAGGGC GCTTTGGGAG GACATTTTTC CTCAAATCAA ACTTTGGGAG 
TTCTACCAGG TCAACGTGGA AAATGCTGTG GAGAAGTTCA GAGTACTGCT GCAAAACGGC 
ACAAAGCCAG ATCATAGCAA GGCTGAAGGA AATAAAGGCA TAGAGATCAT TCAAGACCTC 
CAGTGCTATC GCTATGGCAA CAGAGTGAAC ATGGACTCAG CTTTGGAGAC ATTTGTTCCT 
CACAGCTCCT CTCCACAGCA CATTGATGAG TGCTGTGGTT GGCTGAAACA GAAGTTGACT 
GAGATGAATG AAGAACAGTA CCACATCATA CACCAGCATC AGGAACAGGC CGTCAACTGC 
GTTGTGGGGA ACATAGTGTA TGAACGTCTA GCTGGCCACG GTCCTAAACT GGGTCCTGTT 
ACCAGGAAAG AGCCTCTTGT CACCAGGTAT TTCACCTTCC CCTTTGAGGA AATGACTTTA 
GAGCAGGAGA TGGAGTTTTT AAACCAACCA GATAAGATGT GCCACTTCTT GGCTCACAAC 
GGCTGGGTGA TGGGTGATGA TCCATTGCGC AACTTTGCTG AACCAGGATC CAATGTGTAC 
CTGCGTCGGG AGCTGATCTG CTGGGGGGAC AGTGTCAAAC TCCGCTATGG AAAAGGACCT 
GAGGATTGCC CCTACCTGTG GGAGCACATG CAGAAATACA CTGAAATTAC TGCCAAATAT 
TTCCACGGAG TCCGATTGGA CAACTGCCAC TCTACTCCCT TGCATGTTGC TGAGTTCATG 
TTAGATGCAG CCAGAGAGGT CAGACCCAAC CTGTATGTAA TAGCTGAGCT CTTTACAGGC 
AGTGAACTCA TAGACAATGT GTTTGTTAAC CGACTGGGAA TCAACAGCCT CATAAGAGAG 
GCCATGTCCG CCGGAGACAG CCATGAAGAG GGCCGGCTGG TCTATCGTTA CGGTGGAGAG 
CCAGTCGGAG CCTTCGTTCA GCCCAGCCTG CGTCCTCTCA TGCCTTCTAT CGCTCACGCC 
ATGTTCTTGG ATGTGACCCA TGACAATGAG TGTCCCATTC AGCTGCGGTC AGCTCTCGAC 
TCGTTGCCCA GTTCTGCTAT CGTTTCCATG GCCTGCTGTG CTACTGGCTC CACCAGAGGA 
TATGATGAAC TGGTCCCACA TCAGATATCT GTGGTGAAGG AGGAGCGTTT TTATCCCAAG 
TGGAATCCCT CTTCAGCACC CATCTCTACT GGTGAAGTTG GCTTTCAGAG CGGCATCATC 
GCTGGGAAAC TGGCTCTCAA CAAGCTACAC CAGGAGCTGG CTGCCCAGGG CTTTTCACAG 
GTGTATGTGG ACCAGGTTGA TACAGATATT GTTGCAGTTA CTCGTCACTG CCCCAGCACA 
CACCAGTCTG TGGTAACAGT GTGTAGGACA GCCTTCAAGA ACCCCAAAAC CCATCAGTAC 
AGCAACAATG TCCCACCTAT GTTCATACCT GGCAAGATTG AAGAAATTGT ACTGGAGGCA 
AGGACAGTGG AGAGAGAGGC TGGGAGTTAC AAGAAGGATG AGAAATACAT AAATGGCATG 
CCAGAATACA CAGTGGAAAT AAAAGAGCAT GTACCGATGG TGAAAAGTGC AGTGGTGAAA 
CATGCTGGAG TGACCTCTAA AGACCGCTCA GAGTTTGTGC AAGAAATTCA ATTCCAGAAA 
TTGACTCCTG GTAGCATCAT TGCCTTCAGA GTCTGTTTGG ATCCCAAAGC ACAGAAGCTG 
GTGGGCATGT TGAGGTTTTA TCTGTCCCAG TTCAGTCCAA AGTACAGGAG GGGCAGTGTA 
CCTGATGAGA ATCCACAAGA GGTGCTGCAG AAACCCCTTG CACAGTTAAT GTCTAAGCTG 
ACGCTAGCCG ACATGAACAT CTTGCTGTTT CGCTGCGAAG CAGAAGAACA AGAGGACGGT 
GGAGGCTGTT ACAGCATCCC AGGCTGGGAG GGCCTGAAGT ATTCAGGACT ACAAGGTCTG 
ATATCTGTGT TGGCGGACAT CCGTCCCACT AATGACCTTG GCCATCCTCT GTGTGCTAAC 
CTCAGAGAAG GAGACTGGCT CATAGACTTC GTCTCCAACA GGCTGAAGCA CAGAGATGGA 
CCACTGGCAC AGGTGGGCCA GTGGTTGGGT GCCATGTTTG ACTACCTGAG GCACTTCCCT 
CGATACCTCA TCCCTTGTTA CTTTGACGCC ATCTTGGTGT CAACCTACAC CACAGCTCTG 
GATGCCACGT TCAAACTCAT GTCGAGCTTC ATTCAGCGAG GTTCCACGTT TGTCCGTCAC 
CTGGCTCTCG GCTCAGTTCA GATGTGTGGA GTTGGACGTT TTCCACCTCT GCCTCCCATT 
TCAACAAAAG TGCAGGATGT CCCATACCGC ATCAGTCCCA TCACTGGGGA AAAGGAGCAG 
TGCTGTGTCT CACTGGCAGC AGGTCTTCCT CATTTTTCTG CTGGGATTTT CCGTTGTTGG 
GGTAGAGACA CTTTCATCGC TCTCAGAGGA CTCATGCTTA TAACAGGGAG ACACACTGAG 
GCACGCAACA TCATCCTTGC TTTTGCTGGA ACCCTGCGTC ACGGTTTGAT CCCCAACTTG 
ATGGGAGAGG GTCGCAATGC CAGATACAAC TGCAGGGATG CTGTGTGGTG GTGGCTGCAG 
TGCATTCAGG ACTATACCAA TCTCGTTCCT GAGGGACATG AAATTCTGCG TTGCCCTGTC 
ACCCGCATGT ACCCCACAGA CGACTGTGAG CCCTGCAAAC CAGGGGAGGT GGAGCAGCCA 
TTGTATGATG TTATCCAGGA GGCACTGCAG CGCCATCTGG AGGGTATTTC CTTCAGAGAG 
AGGAATGCTG GACCCAAGAT GGACAGGAAC ATGAGAGATG AAGGGTTCAA TGTGGTTGCT 
AAAGTGGACC CAGCAACAGG TTTTGTTACC GGAGGAAACC GCTTCAACTG TGGCACTTGG 
ATGGATAAAA TGGGGGAGAG TGACAGAGCG AGGAACAAGG GGATACCTGC AACTCCAAGA 
GACGGCGCGG CGGTGGAGAT AGTTGGTCTG AGTAAATCAG CAGTTCGTTG GCTTGTGGAG 
CTGCAGTCTA AAGAACTGTT CCCCTATGAT GGCGTTAAGG TGCACAGAGA TGTGACATGG 
TCTACTGTGG TGTTTACGAT AATGCACTGG ACAACGACAA CTACAACCTG GCCAAGGGCT 
TTAACTACCA CCAAGGACCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008431726.1
CDS	201..4364
Translation	MLLLTGFFFSLRLLIVICLMPVSLETRVPSKFRRTSMMLPQHLKQIRVLMLNEKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYADVERLGGGYVVVDPILRVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSYTWEDVGRLVEKLRTEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNESHLNALRRALWEDIFPQIKLWEFYQVNVENAVEKFRVLLQNGTKPDHSKAEGNKGIEIIQDLQCYRYGNRVNMDSALETFVPHSSSPQHIDECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCVVGNIVYERLAGHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWEHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDAAREVRPNLYVIAELFTGSELIDNVFVNRLGINSLIREAMSAGDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIQLRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPISTGEVGFQSGIIAGKLALNKLHQELAAQGFSQVYVDQVDTDIVAVTRHCPSTHQSVVTVCRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREAGSYKKDEKYINGMPEYTVEIKEHVPMVKSAVVKHAGVTSKDRSEFVQEIQFQKLTPGSIIAFRVCLDPKAQKLVGMLRFYLSQFSPKYRRGSVPDENPQEVLQKPLAQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWEGLKYSGLQGLISVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAMFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIQRGSTFVRHLALGSVQMCGVGRFPPLPPISTKVQDVPYRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLMGEGRNARYNCRDAVWWWLQCIQDYTNLVPEGHEILRCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKMDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGIPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVHRDVTWSTVVFTIMHWTTTTTTWPRALTTTKDL

Target ORF information:

RefSeq Version	XM_008433504.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008433504.1

ORF Insert Sequence:

ATGCTGCTAC TTACAGGTTT CTTCTTCAGT TTGAGACTCC TCATTGTAAT CTGTCTAATG 
CCCGTCTCTT TGGAGACCAG GGTTCCCTCC AAGTTCAGGA GAACTTCCAT GATGCTGCCT 
CAGCACCTAA AGCAAATCAG AGTACTCATG CTTAACGAAA AGGAGAATTT AGAGAGAACT 
TTGTTCAGAC TTGAACAAGG TTTTGAGCTC CAGTTCCGCC TGGGTCCAAG CCTTCAAGGA 
AGGAGAGTTA TAGTTCACAC CAACTACCCA CTTGATGGAC AGAAGTTCAT TCGAAACAAC 
TTCCGTGTTT TAGCCTGGAA CTACCCAACA GGAAGGGAGG ATGACTCTGA TAAGTACTGC 
TCCCTAGAGC TCAAGATTGC TGGATCCTAC CAGTACTACT TTGGATATGC AGATGTGGAG 
AGGTTAGGAG GAGGCTACGT CGTCGTTGAT CCTATTCTTC GTGTTGGAGC AGACAACCAT 
GTCCTCCTGC TGGACTGTGT CACCATCCAG ACATACTTGT CCAAATGTCT GGGACGCCTT 
GATGACTGGC CAGACAGACT GCGAGTAGCC AAGGAGTCAG GTTACAACAT GATCCACTTC 
ACTCCTCTGC AGACTTTGGG AGAGTCTAGG TCCTGTTACT CCTTAGCTGA CCAGCTGACC 
TTTAGCCTGG AATTCAGCCC AGATGGCAAG AGTTACACCT GGGAAGATGT GGGAAGGCTA 
GTGGAAAAAC TGAGGACGGA ATGGAATATG GTATCAATCA CTGATGTAGT GTATAATCAC 
ACTGCTGCAA ACAGTGTGTG GATAAAGGAA CATCCAGAGT GTGGCTACAA CCTGATGAAC 
TCTCCTCACC TGCGTCCTGC CTGGGTCTTA GACAGAGCTA TCTGGCATTT AACCACTAGG 
GTAGCAGATG GACGGTACAA AGAGAAGGGA CTTCCTTCTG ACATTACCAA CGAGAGCCAC 
CTAAATGCTC TCCGCAGGGC GCTTTGGGAG GACATTTTTC CTCAAATCAA ACTTTGGGAG 
TTCTACCAGG TCAACGTGGA AAATGCTGTG GAGAAGTTCA GAGTACTGCT GCAAAACGGC 
ACAAAGCCAG ATCATAGCAA GGCTGAAGGA AATAAAGGCA TAGAGATCAT TCAAGACCTC 
CAGTGCTATC GCTATGGCAA CAGAGTGAAC ATGGACTCAG CTTTGGAGAC ATTTGTTCCT 
CACAGCTCCT CTCCACAGCA CATTGATGAG TGCTGTGGTT GGCTGAAACA GAAGTTGACT 
GAGATGAATG AAGAACAGTA CCACATCATA CACCAGCATC AGGAACAGGC CGTCAACTGC 
GTTGTGGGGA ACATAGTGTA TGAACGTCTA GCTGGCCACG GTCCTAAACT GGGTCCTGTT 
ACCAGGAAAG AGCCTCTTGT CACCAGGTAT TTCACCTTCC CCTTTGAGGA AATGACTTTA 
GAGCAGGAGA TGGAGTTTTT AAACCAACCA GATAAGATGT GCCACTTCTT GGCTCACAAC 
GGCTGGGTGA TGGGTGATGA TCCATTGCGC AACTTTGCTG AACCAGGATC CAATGTGTAC 
CTGCGTCGGG AGCTGATCTG CTGGGGGGAC AGTGTCAAAC TCCGCTATGG AAAAGGACCT 
GAGGATTGCC CCTACCTGTG GGAGCACATG CAGAAATACA CTGAAATTAC TGCCAAATAT 
TTCCACGGAG TCCGATTGGA CAACTGCCAC TCTACTCCCT TGCATGTTGC TGAGTTCATG 
TTAGATGCAG CCAGAGAGGT CAGACCCAAC CTGTATGTAA TAGCTGAGCT CTTTACAGGC 
AGTGAACTCA TAGACAATGT GTTTGTTAAC CGACTGGGAA TCAACAGCCT CATAAGAGAG 
GCCATGTCCG CCGGAGACAG CCATGAAGAG GGCCGGCTGG TCTATCGTTA CGGTGGAGAG 
CCAGTCGGAG CCTTCGTTCA GCCCAGCCTG CGTCCTCTCA TGCCTTCTAT CGCTCACGCC 
ATGTTCTTGG ATGTGACCCA TGACAATGAG TGTCCCATTC AGCTGCGGTC AGCTCTCGAC 
TCGTTGCCCA GTTCTGCTAT CGTTTCCATG GCCTGCTGTG CTACTGGCTC CACCAGAGGA 
TATGATGAAC TGGTCCCACA TCAGATATCT GTGGTGAAGG AGGAGCGTTT TTATCCCAAG 
TGGAATCCCT CTTCAGCACC CATCTCTACT GGTGAAGTTG GCTTTCAGAG CGGCATCATC 
GCTGGGAAAC TGGCTCTCAA CAAGCTACAC CAGGAGCTGG CTGCCCAGGG CTTTTCACAG 
GTGTATGTGG ACCAGGTTGA TACAGATATT GTTGCAGTTA CTCGTCACTG CCCCAGCACA 
CACCAGTCTG TGGTAACAGT GTGTAGGACA GCCTTCAAGA ACCCCAAAAC CCATCAGTAC 
AGCAACAATG TCCCACCTAT GTTCATACCT GGCAAGATTG AAGAAATTGT ACTGGAGGCA 
AGGACAGTGG AGAGAGAGGC TGGGAGTTAC AAGAAGGATG AGAAATACAT AAATGGCATG 
CCAGAATACA CAGTGGAAAT AAAAGAGCAT GTACCGATGG TGAAAAGTGC AGTGGTGAAA 
CATGCTGGAG TGACCTCTAA AGACCGCTCA GAGTTTGTGC AAGAAATTCA ATTCCAGAAA 
TTGACTCCTG GTAGCATCAT TGCCTTCAGA GTCTGTTTGG ATCCCAAAGC ACAGAAGCTG 
GTGGGCATGT TGAGGTTTTA TCTGTCCCAG TTCAGTCCAA AGTACAGGAG GGGCAGTGTA 
CCTGATGAGA ATCCACAAGA GGTGCTGCAG AAACCCCTTG CACAGTTAAT GTCTAAGCTG 
ACGCTAGCCG ACATGAACAT CTTGCTGTTT CGCTGCGAAG CAGAAGAACA AGAGGACGGT 
GGAGGCTGTT ACAGCATCCC AGGCTGGGAG GGCCTGAAGT ATTCAGGACT ACAAGGTCTG 
ATATCTGTGT TGGCGGACAT CCGTCCCACT AATGACCTTG GCCATCCTCT GTGTGCTAAC 
CTCAGAGAAG GAGACTGGCT CATAGACTTC GTCTCCAACA GGCTGAAGCA CAGAGATGGA 
CCACTGGCAC AGGTGGGCCA GTGGTTGGGT GCCATGTTTG ACTACCTGAG GCACTTCCCT 
CGATACCTCA TCCCTTGTTA CTTTGACGCC ATCTTGGTGT CAACCTACAC CACAGCTCTG 
GATGCCACGT TCAAACTCAT GTCGAGCTTC ATTCAGCGAG GTTCCACGTT TGTCCGTCAC 
CTGGCTCTCG GCTCAGTTCA GATGTGTGGA GTTGGACGTT TTCCACCTCT GCCTCCCATT 
TCAACAAAAG TGCAGGATGT CCCATACCGC ATCAGTCCCA TCACTGGGGA AAAGGAGCAG 
TGCTGTGTCT CACTGGCAGC AGGTCTTCCT CATTTTTCTG CTGGGATTTT CCGTTGTTGG 
GGTAGAGACA CTTTCATCGC TCTCAGAGGA CTCATGCTTA TAACAGGGAG ACACACTGAG 
GCACGCAACA TCATCCTTGC TTTTGCTGGA ACCCTGCGTC ACGGTTTGAT CCCCAACTTG 
ATGGGAGAGG GTCGCAATGC CAGATACAAC TGCAGGGATG CTGTGTGGTG GTGGCTGCAG 
TGCATTCAGG ACTATACCAA TCTCGTTCCT GAGGGACATG AAATTCTGCG TTGCCCTGTC 
ACCCGCATGT ACCCCACAGA CGACTGTGAG CCCTGCAAAC CAGGGGAGGT GGAGCAGCCA 
TTGTATGATG TTATCCAGGA GGCACTGCAG CGCCATCTGG AGGGTATTTC CTTCAGAGAG 
AGGAATGCTG GACCCAAGAT GGACAGGAAC ATGAGAGATG AAGGGTTCAA TGTGGTTGCT 
AAAGTGGACC CAGCAACAGG TTTTGTTACC GGAGGAAACC GCTTCAACTG TGGCACTTGG 
ATGGATAAAA TGGGGGAGAG TGACAGAGCG AGGAACAAGG GGATACCTGC AACTCCAAGA 
GACGGCGCGG CGGTGGAGAT AGTTGGTCTG AGTAAATCAG CAGTTCGTTG GCTTGTGGAG 
CTGCAGTCTA AAGAACTGTT CCCCTATGAT GGCGTTAAGG TGCACAGAGA TGTGACATGG 
TCTACTGTGG TGTTTACGAT AATGCACTGG ACAACGACAA CTACAACCTG GCCAAGGGCT 
TTAACTACCA CCAAGGACCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_008433499.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008431721.1 Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo179977
Clone ID Related Accession (Same CDS sequence)	XM_008433499.2
Accession Version	XM_008433499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008433499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTAC TTACAGGTTT CTTCTTCAGT TTGAGACTCC TCATTGTAAT CTGTCTAATG 
CCCGTCTCTT TGGAGACCAG GGTTCCCTCC AAGTTCAGGA GAACTTCCAT GATGCTGCCT 
CAGCACCTAA AGCAAATCAG AGTACTCATG CTTAACGAAA AGGAGAATTT AGAGAGAACT 
TTGTTCAGAC TTGAACAAGG TTTTGAGCTC CAGTTCCGCC TGGGTCCAAG CCTTCAAGGA 
AGGAGAGTTA TAGTTCACAC CAACTACCCA CTTGATGGAC AGAAGTTCAT TCGAAACAAC 
TTCCGTGTTT TAGCCTGGAA CTACCCAACA GGAAGGGAGG ATGACTCTGA TAAGTACTGC 
TCCCTAGAGC TCAAGATTGC TGGATCCTAC CAGTACTACT TTGGATATGC AGATGTGGAG 
AGGTTAGGAG GAGGCTACGT CGTCGTTGAT CCTATTCTTC GTGTTGGAGC AGACAACCAT 
GTCCTCCTGC TGGACTGTGT CACCATCCAG ACATACTTGT CCAAATGTCT GGGACGCCTT 
GATGACTGGC CAGACAGACT GCGAGTAGCC AAGGAGTCAG GTTACAACAT GATCCACTTC 
ACTCCTCTGC AGACTTTGGG AGAGTCTAGG TCCTGTTACT CCTTAGCTGA CCAGCTGACC 
TTTAGCCTGG AATTCAGCCC AGATGGCAAG AGTTACACCT GGGAAGATGT GGGAAGGCTA 
GTGGAAAAAC TGAGGACGGA ATGGAATATG GTATCAATCA CTGATGTAGT GTATAATCAC 
ACTGCTGCAA ACAGTGTGTG GATAAAGGAA CATCCAGAGT GTGGCTACAA CCTGATGAAC 
TCTCCTCACC TGCGTCCTGC CTGGGTCTTA GACAGAGCTA TCTGGCATTT AACCACTAGG 
GTAGCAGATG GACGGTACAA AGAGAAGGGA CTTCCTTCTG ACATTACCAA CGAGAGCCAC 
CTAAATGCTC TCCGCAGGGC GCTTTGGGAG GACATTTTTC CTCAAATCAA ACTTTGGGAG 
TTCTACCAGG TCAACGTGGA AAATGCTGTG GAGAAGTTCA GAGTACTGCT GCAAAACGGC 
ACAAAGCCAG ATCATAGCAA GGCTGAAGGA AATAAAGGCA TAGAGATCAT TCAAGACCTC 
CAGTGCTATC GCTATGGCAA CAGAGTGAAC ATGGACTCAG CTTTGGAGAC ATTTGTTCCT 
CACAGCTCCT CTCCACAGCA CATTGATGAG TGCTGTGGTT GGCTGAAACA GAAGTTGACT 
GAGATGAATG AAGAACAGTA CCACATCATA CACCAGCATC AGGAACAGGC CGTCAACTGC 
GTTGTGGGGA ACATAGTGTA TGAACGTCTA GCTGGCCACG GTCCTAAACT GGGTCCTGTT 
ACCAGGAAAG AGCCTCTTGT CACCAGGTAT TTCACCTTCC CCTTTGAGGA AATGACTTTA 
GAGCAGGAGA TGGAGTTTTT AAACCAACCA GATAAGATGT GCCACTTCTT GGCTCACAAC 
GGCTGGGTGA TGGGTGATGA TCCATTGCGC AACTTTGCTG AACCAGGATC CAATGTGTAC 
CTGCGTCGGG AGCTGATCTG CTGGGGGGAC AGTGTCAAAC TCCGCTATGG AAAAGGACCT 
GAGGATTGCC CCTACCTGTG GGAGCACATG CAGAAATACA CTGAAATTAC TGCCAAATAT 
TTCCACGGAG TCCGATTGGA CAACTGCCAC TCTACTCCCT TGCATGTTGC TGAGTTCATG 
TTAGATGCAG CCAGAGAGGT CAGACCCAAC CTGTATGTAA TAGCTGAGCT CTTTACAGGC 
AGTGAACTCA TAGACAATGT GTTTGTTAAC CGACTGGGAA TCAACAGCCT CATAAGAGAG 
GCCATGTCCG CCGGAGACAG CCATGAAGAG GGCCGGCTGG TCTATCGTTA CGGTGGAGAG 
CCAGTCGGAG CCTTCGTTCA GCCCAGCCTG CGTCCTCTCA TGCCTTCTAT CGCTCACGCC 
ATGTTCTTGG ATGTGACCCA TGACAATGAG TGTCCCATTC AGCTGCGGTC AGCTCTCGAC 
TCGTTGCCCA GTTCTGCTAT CGTTTCCATG GCCTGCTGTG CTACTGGCTC CACCAGAGGA 
TATGATGAAC TGGTCCCACA TCAGATATCT GTGGTGAAGG AGGAGCGTTT TTATCCCAAG 
TGGAATCCCT CTTCAGCACC CATCTCTACT GGTGAAGTTG GCTTTCAGAG CGGCATCATC 
GCTGGGAAAC TGGCTCTCAA CAAGCTACAC CAGGAGCTGG CTGCCCAGGG CTTTTCACAG 
GTGTATGTGG ACCAGGTTGA TACAGATATT GTTGCAGTTA CTCGTCACTG CCCCAGCACA 
CACCAGTCTG TGGTAACAGT GTGTAGGACA GCCTTCAAGA ACCCCAAAAC CCATCAGTAC 
AGCAACAATG TCCCACCTAT GTTCATACCT GGCAAGATTG AAGAAATTGT ACTGGAGGCA 
AGGACAGTGG AGAGAGAGGC TGGGAGTTAC AAGAAGGATG AGAAATACAT AAATGGCATG 
CCAGAATACA CAGTGGAAAT AAAAGAGCAT GTACCGATGG TGAAAAGTGC AGTGGTGAAA 
CATGCTGGAG TGACCTCTAA AGACCGCTCA GAGTTTGTGC AAGAAATTCA ATTCCAGAAA 
TTGACTCCTG GTAGCATCAT TGCCTTCAGA GTCTGTTTGG ATCCCAAAGC ACAGAAGCTG 
GTGGGCATGT TGAGGTTTTA TCTGTCCCAG TTCAGTCCAA AGTACAGGAG GGGCAGTGTA 
CCTGATGAGA ATCCACAAGA GGTGCTGCAG AAACCCCTTG CACAGTTAAT GTCTAAGCTG 
ACGCTAGCCG ACATGAACAT CTTGCTGTTT CGCTGCGAAG CAGAAGAACA AGAGGACGGT 
GGAGGCTGTT ACAGCATCCC AGGCTGGGAG GGCCTGAAGT ATTCAGGACT ACAAGGTCTG 
ATATCTGTGT TGGCGGACAT CCGTCCCACT AATGACCTTG GCCATCCTCT GTGTGCTAAC 
CTCAGAGAAG GAGACTGGCT CATAGACTTC GTCTCCAACA GGCTGAAGCA CAGAGATGGA 
CCACTGGCAC AGGTGGGCCA GTGGTTGGGT GCCATGTTTG ACTACCTGAG GCACTTCCCT 
CGATACCTCA TCCCTTGTTA CTTTGACGCC ATCTTGGTGT CAACCTACAC CACAGCTCTG 
GATGCCACGT TCAAACTCAT GTCGAGCTTC ATTCAGCGAG GTTCCACGTT TGTCCGTCAC 
CTGGCTCTCG GCTCAGTTCA GATGTGTGGA GTTGGACGTT TTCCACCTCT GCCTCCCATT 
TCAACAAAAG TGCAGGATGT CCCATACCGC ATCAGTCCCA TCACTGGGGA AAAGGAGCAG 
TGCTGTGTCT CACTGGCAGC AGGTCTTCCT CATTTTTCTG CTGGGATTTT CCGTTGTTGG 
GGTAGAGACA CTTTCATCGC TCTCAGAGGA CTCATGCTTA TAACAGGGAG ACACACTGAG 
GCACGCAACA TCATCCTTGC TTTTGCTGGA ACCCTGCGTC ACGGTTTGAT CCCCAACTTG 
ATGGGAGAGG GTCGCAATGC CAGATACAAC TGCAGGGATG CTGTGTGGTG GTGGCTGCAG 
TGCATTCAGG ACTATACCAA TCTCGTTCCT GAGGGACATG AAATTCTGCG TTGCCCTGTC 
ACCCGCATGT ACCCCACAGA CGACTGTGAG CCCTGCAAAC CAGGGGAGGT GGAGCAGCCA 
TTGTATGATG TTATCCAGGA GGCACTGCAG CGCCATCTGG AGGGTATTTC CTTCAGAGAG 
AGGAATGCTG GACCCAAGAT GGACAGGAAC ATGAGAGATG AAGGGTTCAA TGTGGTTGCT 
AAAGTGGACC CAGCAACAGG TTTTGTTACC GGAGGAAACC GCTTCAACTG TGGCACTTGG 
ATGGATAAAA TGGGGGAGAG TGACAGAGCG AGGAACAAGG GGATACCTGC AACTCCAAGA 
GACGGCGCGG CGGTGGAGAT AGTTGGTCTG AGTAAATCAG CAGTTCGTTG GCTTGTGGAG 
CTGCAGTCTA AAGAACTGTT CCCCTATGAT GGCGTTAAGG TGCACAGAGA TGGAAAGGAT 
GTGTTCATCT TGTATACTGA GTGGAACAAG CAGCTTCAGG AGTCCTTTGA AGTTGAATTC 
TGGGTGTCTG GGGACTCACA GGACCCCAAT GAGAAGCACC CTGATTTGGT GCATAAAAAG 
GGAATTTATA AAGATAGTTA TGGAGCTTCC AATCCCTGGT GTGACTACCA GCTGAGGCCC 
AACTTTACCA TCGCAATGGT GGTGGCTCCA GAGCTGTTCA CGCTAGAAAG AGCATGGAAG 
TCTTTGGAAA TAGCTGAAAA GAAACTCCTG GGACCACTGG GAATGAAAAC TCTTGACCCA 
GATGACATGG TCTACTGTGG TGTTTACGAT AATGCACTGG ACAACGACAA CTACAACCTG 
GCCAAGGGCT TTAACTACCA CCAAGGACCT GAGTGGCTGT GGCCTGTTGG CTATTTTCTC 
CGTGCTAAGC TCTACTTTGC CAAGAGGTTA GGGGAGGAGA CCTACTCCAA GACCGTCATC 
CTAGTGAAAA ATGTTCTGTC ACGACATTGC ACCCACTTGG AGAGGTCTCT ATGGAAAGGC 
TTACCAGAGC TGACCAATGA AAACGGCCAG CATTGCCCCT TCAGCTGTGA GACCCAGGCC 
TGGTCTCTGG CTACTGTGCT GGAGGTGCTC CATGATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008431721.1
CDS	198..4919
Translation	MLLLTGFFFSLRLLIVICLMPVSLETRVPSKFRRTSMMLPQHLKQIRVLMLNEKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYADVERLGGGYVVVDPILRVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSYTWEDVGRLVEKLRTEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNESHLNALRRALWEDIFPQIKLWEFYQVNVENAVEKFRVLLQNGTKPDHSKAEGNKGIEIIQDLQCYRYGNRVNMDSALETFVPHSSSPQHIDECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCVVGNIVYERLAGHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWEHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDAAREVRPNLYVIAELFTGSELIDNVFVNRLGINSLIREAMSAGDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIQLRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPISTGEVGFQSGIIAGKLALNKLHQELAAQGFSQVYVDQVDTDIVAVTRHCPSTHQSVVTVCRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREAGSYKKDEKYINGMPEYTVEIKEHVPMVKSAVVKHAGVTSKDRSEFVQEIQFQKLTPGSIIAFRVCLDPKAQKLVGMLRFYLSQFSPKYRRGSVPDENPQEVLQKPLAQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWEGLKYSGLQGLISVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAMFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIQRGSTFVRHLALGSVQMCGVGRFPPLPPISTKVQDVPYRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLMGEGRNARYNCRDAVWWWLQCIQDYTNLVPEGHEILRCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKMDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGIPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVHRDGKDVFILYTEWNKQLQESFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKSLEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKRLGEETYSKTVILVKNVLSRHCTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_008433499.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008433499.2

ORF Insert Sequence:

ATGCTGCTAC TTACAGGTTT CTTCTTCAGT TTGAGACTCC TCATTGTAAT CTGTCTAATG 
CCCGTCTCTT TGGAGACCAG GGTTCCCTCC AAGTTCAGGA GAACTTCCAT GATGCTGCCT 
CAGCACCTAA AGCAAATCAG AGTACTCATG CTTAACGAAA AGGAGAATTT AGAGAGAACT 
TTGTTCAGAC TTGAACAAGG TTTTGAGCTC CAGTTCCGCC TGGGTCCAAG CCTTCAAGGA 
AGGAGAGTTA TAGTTCACAC CAACTACCCA CTTGATGGAC AGAAGTTCAT TCGAAACAAC 
TTCCGTGTTT TAGCCTGGAA CTACCCAACA GGAAGGGAGG ATGACTCTGA TAAGTACTGC 
TCCCTAGAGC TCAAGATTGC TGGATCCTAC CAGTACTACT TTGGATATGC AGATGTGGAG 
AGGTTAGGAG GAGGCTACGT CGTCGTTGAT CCTATTCTTC GTGTTGGAGC AGACAACCAT 
GTCCTCCTGC TGGACTGTGT CACCATCCAG ACATACTTGT CCAAATGTCT GGGACGCCTT 
GATGACTGGC CAGACAGACT GCGAGTAGCC AAGGAGTCAG GTTACAACAT GATCCACTTC 
ACTCCTCTGC AGACTTTGGG AGAGTCTAGG TCCTGTTACT CCTTAGCTGA CCAGCTGACC 
TTTAGCCTGG AATTCAGCCC AGATGGCAAG AGTTACACCT GGGAAGATGT GGGAAGGCTA 
GTGGAAAAAC TGAGGACGGA ATGGAATATG GTATCAATCA CTGATGTAGT GTATAATCAC 
ACTGCTGCAA ACAGTGTGTG GATAAAGGAA CATCCAGAGT GTGGCTACAA CCTGATGAAC 
TCTCCTCACC TGCGTCCTGC CTGGGTCTTA GACAGAGCTA TCTGGCATTT AACCACTAGG 
GTAGCAGATG GACGGTACAA AGAGAAGGGA CTTCCTTCTG ACATTACCAA CGAGAGCCAC 
CTAAATGCTC TCCGCAGGGC GCTTTGGGAG GACATTTTTC CTCAAATCAA ACTTTGGGAG 
TTCTACCAGG TCAACGTGGA AAATGCTGTG GAGAAGTTCA GAGTACTGCT GCAAAACGGC 
ACAAAGCCAG ATCATAGCAA GGCTGAAGGA AATAAAGGCA TAGAGATCAT TCAAGACCTC 
CAGTGCTATC GCTATGGCAA CAGAGTGAAC ATGGACTCAG CTTTGGAGAC ATTTGTTCCT 
CACAGCTCCT CTCCACAGCA CATTGATGAG TGCTGTGGTT GGCTGAAACA GAAGTTGACT 
GAGATGAATG AAGAACAGTA CCACATCATA CACCAGCATC AGGAACAGGC CGTCAACTGC 
GTTGTGGGGA ACATAGTGTA TGAACGTCTA GCTGGCCACG GTCCTAAACT GGGTCCTGTT 
ACCAGGAAAG AGCCTCTTGT CACCAGGTAT TTCACCTTCC CCTTTGAGGA AATGACTTTA 
GAGCAGGAGA TGGAGTTTTT AAACCAACCA GATAAGATGT GCCACTTCTT GGCTCACAAC 
GGCTGGGTGA TGGGTGATGA TCCATTGCGC AACTTTGCTG AACCAGGATC CAATGTGTAC 
CTGCGTCGGG AGCTGATCTG CTGGGGGGAC AGTGTCAAAC TCCGCTATGG AAAAGGACCT 
GAGGATTGCC CCTACCTGTG GGAGCACATG CAGAAATACA CTGAAATTAC TGCCAAATAT 
TTCCACGGAG TCCGATTGGA CAACTGCCAC TCTACTCCCT TGCATGTTGC TGAGTTCATG 
TTAGATGCAG CCAGAGAGGT CAGACCCAAC CTGTATGTAA TAGCTGAGCT CTTTACAGGC 
AGTGAACTCA TAGACAATGT GTTTGTTAAC CGACTGGGAA TCAACAGCCT CATAAGAGAG 
GCCATGTCCG CCGGAGACAG CCATGAAGAG GGCCGGCTGG TCTATCGTTA CGGTGGAGAG 
CCAGTCGGAG CCTTCGTTCA GCCCAGCCTG CGTCCTCTCA TGCCTTCTAT CGCTCACGCC 
ATGTTCTTGG ATGTGACCCA TGACAATGAG TGTCCCATTC AGCTGCGGTC AGCTCTCGAC 
TCGTTGCCCA GTTCTGCTAT CGTTTCCATG GCCTGCTGTG CTACTGGCTC CACCAGAGGA 
TATGATGAAC TGGTCCCACA TCAGATATCT GTGGTGAAGG AGGAGCGTTT TTATCCCAAG 
TGGAATCCCT CTTCAGCACC CATCTCTACT GGTGAAGTTG GCTTTCAGAG CGGCATCATC 
GCTGGGAAAC TGGCTCTCAA CAAGCTACAC CAGGAGCTGG CTGCCCAGGG CTTTTCACAG 
GTGTATGTGG ACCAGGTTGA TACAGATATT GTTGCAGTTA CTCGTCACTG CCCCAGCACA 
CACCAGTCTG TGGTAACAGT GTGTAGGACA GCCTTCAAGA ACCCCAAAAC CCATCAGTAC 
AGCAACAATG TCCCACCTAT GTTCATACCT GGCAAGATTG AAGAAATTGT ACTGGAGGCA 
AGGACAGTGG AGAGAGAGGC TGGGAGTTAC AAGAAGGATG AGAAATACAT AAATGGCATG 
CCAGAATACA CAGTGGAAAT AAAAGAGCAT GTACCGATGG TGAAAAGTGC AGTGGTGAAA 
CATGCTGGAG TGACCTCTAA AGACCGCTCA GAGTTTGTGC AAGAAATTCA ATTCCAGAAA 
TTGACTCCTG GTAGCATCAT TGCCTTCAGA GTCTGTTTGG ATCCCAAAGC ACAGAAGCTG 
GTGGGCATGT TGAGGTTTTA TCTGTCCCAG TTCAGTCCAA AGTACAGGAG GGGCAGTGTA 
CCTGATGAGA ATCCACAAGA GGTGCTGCAG AAACCCCTTG CACAGTTAAT GTCTAAGCTG 
ACGCTAGCCG ACATGAACAT CTTGCTGTTT CGCTGCGAAG CAGAAGAACA AGAGGACGGT 
GGAGGCTGTT ACAGCATCCC AGGCTGGGAG GGCCTGAAGT ATTCAGGACT ACAAGGTCTG 
ATATCTGTGT TGGCGGACAT CCGTCCCACT AATGACCTTG GCCATCCTCT GTGTGCTAAC 
CTCAGAGAAG GAGACTGGCT CATAGACTTC GTCTCCAACA GGCTGAAGCA CAGAGATGGA 
CCACTGGCAC AGGTGGGCCA GTGGTTGGGT GCCATGTTTG ACTACCTGAG GCACTTCCCT 
CGATACCTCA TCCCTTGTTA CTTTGACGCC ATCTTGGTGT CAACCTACAC CACAGCTCTG 
GATGCCACGT TCAAACTCAT GTCGAGCTTC ATTCAGCGAG GTTCCACGTT TGTCCGTCAC 
CTGGCTCTCG GCTCAGTTCA GATGTGTGGA GTTGGACGTT TTCCACCTCT GCCTCCCATT 
TCAACAAAAG TGCAGGATGT CCCATACCGC ATCAGTCCCA TCACTGGGGA AAAGGAGCAG 
TGCTGTGTCT CACTGGCAGC AGGTCTTCCT CATTTTTCTG CTGGGATTTT CCGTTGTTGG 
GGTAGAGACA CTTTCATCGC TCTCAGAGGA CTCATGCTTA TAACAGGGAG ACACACTGAG 
GCACGCAACA TCATCCTTGC TTTTGCTGGA ACCCTGCGTC ACGGTTTGAT CCCCAACTTG 
ATGGGAGAGG GTCGCAATGC CAGATACAAC TGCAGGGATG CTGTGTGGTG GTGGCTGCAG 
TGCATTCAGG ACTATACCAA TCTCGTTCCT GAGGGACATG AAATTCTGCG TTGCCCTGTC 
ACCCGCATGT ACCCCACAGA CGACTGTGAG CCCTGCAAAC CAGGGGAGGT GGAGCAGCCA 
TTGTATGATG TTATCCAGGA GGCACTGCAG CGCCATCTGG AGGGTATTTC CTTCAGAGAG 
AGGAATGCTG GACCCAAGAT GGACAGGAAC ATGAGAGATG AAGGGTTCAA TGTGGTTGCT 
AAAGTGGACC CAGCAACAGG TTTTGTTACC GGAGGAAACC GCTTCAACTG TGGCACTTGG 
ATGGATAAAA TGGGGGAGAG TGACAGAGCG AGGAACAAGG GGATACCTGC AACTCCAAGA 
GACGGCGCGG CGGTGGAGAT AGTTGGTCTG AGTAAATCAG CAGTTCGTTG GCTTGTGGAG 
CTGCAGTCTA AAGAACTGTT CCCCTATGAT GGCGTTAAGG TGCACAGAGA TGGAAAGGAT 
GTGTTCATCT TGTATACTGA GTGGAACAAG CAGCTTCAGG AGTCCTTTGA AGTTGAATTC 
TGGGTGTCTG GGGACTCACA GGACCCCAAT GAGAAGCACC CTGATTTGGT GCATAAAAAG 
GGAATTTATA AAGATAGTTA TGGAGCTTCC AATCCCTGGT GTGACTACCA GCTGAGGCCC 
AACTTTACCA TCGCAATGGT GGTGGCTCCA GAGCTGTTCA CGCTAGAAAG AGCATGGAAG 
TCTTTGGAAA TAGCTGAAAA GAAACTCCTG GGACCACTGG GAATGAAAAC TCTTGACCCA 
GATGACATGG TCTACTGTGG TGTTTACGAT AATGCACTGG ACAACGACAA CTACAACCTG 
GCCAAGGGCT TTAACTACCA CCAAGGACCT GAGTGGCTGT GGCCTGTTGG CTATTTTCTC 
CGTGCTAAGC TCTACTTTGC CAAGAGGTTA GGGGAGGAGA CCTACTCCAA GACCGTCATC 
CTAGTGAAAA ATGTTCTGTC ACGACATTGC ACCCACTTGG AGAGGTCTCT ATGGAAAGGC 
TTACCAGAGC TGACCAATGA AAACGGCCAG CATTGCCCCT TCAGCTGTGA GACCCAGGCC 
TGGTCTCTGG CTACTGTGCT GGAGGTGCTC CATGATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_008433500.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008431722.1 Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA.

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CloneID	OPo179978
Clone ID Related Accession (Same CDS sequence)	XM_008433500.2
Accession Version	XM_008433500.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008433500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTAC TTACAGGTTT CTTCTTCAGT TTGAGACTCC TCATTGTAAT CTGTCTAATG 
CCCGTCTCTT TGGAGACCAG GGTTCCCTCC AAGTTCAGGA GAACTTCCAT GATGCTGCCT 
CAGCACCTAA AGCAAATCAG AGTACTCATG CTTAACGAAA AGGAGAATTT AGAGAGAACT 
TTGTTCAGAC TTGAACAAGG TTTTGAGCTC CAGTTCCGCC TGGGTCCAAG CCTTCAAGGA 
AGGAGAGTTA TAGTTCACAC CAACTACCCA CTTGATGGAC AGAAGTTCAT TCGAAACAAC 
TTCCGTGTTT TAGCCTGGAA CTACCCAACA GGAAGGGAGG ATGACTCTGA TAAGTACTGC 
TCCCTAGAGC TCAAGATTGC TGGATCCTAC CAGTACTACT TTGGATATGC AGATGTGGAG 
AGGTTAGGAG GAGGCTACGT CGTCGTTGAT CCTATTCTTC GTGTTGGAGC AGACAACCAT 
GTCCTCCTGC TGGACTGTGT CACCATCCAG ACATACTTGT CCAAATGTCT GGGACGCCTT 
GATGACTGGC CAGACAGACT GCGAGTAGCC AAGGAGTCAG GTTACAACAT GATCCACTTC 
ACTCCTCTGC AGACTTTGGG AGAGTCTAGG TCCTGTTACT CCTTAGCTGA CCAGCTGACC 
TTTAGCCTGG AATTCAGCCC AGATGGCAAG AGTTACACCT GGGAAGATGT GGGAAGGCTA 
GTGGAAAAAC TGAGGACGGA ATGGAATATG GTATCAATCA CTGATGTAGT GTATAATCAC 
ACTGCTGCAA ACAGTGTGTG GATAAAGGAA CATCCAGAGT GTGGCTACAA CCTGATGAAC 
TCTCCTCACC TGCGTCCTGC CTGGGTCTTA GACAGAGCTA TCTGGCATTT AACCACTAGG 
GTAGCAGATG GACGGTACAA AGAGAAGGGA CTTCCTTCTG ACATTACCAA CGAGAGCCAC 
CTAAATGCTC TCCGCAGGGC GCTTTGGGAG GACATTTTTC CTCAAATCAA ACTTTGGGAG 
TTCTACCAGG TCAACGTGGA AAATGCTGTG GAGAAGTTCA GAGTACTGCT GCAAAACGGC 
ACAAAGCCAG ATCATAGCAA GGCTGAAGGA AATAAAGGCA TAGAGATCAT TCAAGACCTC 
CAGTGCTATC GCTATGGCAA CAGAGTGAAC ATGGACTCAG CTTTGGAGAC ATTTGTTCCT 
CACAGCTCCT CTCCACAGCA CATTGATGAG TGCTGTGGTT GGCTGAAACA GAAGTTGACT 
GAGATGAATG AAGAACAGTA CCACATCATA CACCAGCATC AGGAACAGGC CGTCAACTGC 
GTTGTGGGGA ACATAGTGTA TGAACGTCTA GCTGGCCACG GTCCTAAACT GGGTCCTGTT 
ACCAGGAAAG AGCCTCTTGT CACCAGGTAT TTCACCTTCC CCTTTGAGGA AATGACTTTA 
GAGCAGGAGA TGGAGTTTTT AAACCAACCA GATAAGATGT GCCACTTCTT GGCTCACAAC 
GGCTGGGTGA TGGGTGATGA TCCATTGCGC AACTTTGCTG AACCAGGATC CAATGTGTAC 
CTGCGTCGGG AGCTGATCTG CTGGGGGGAC AGTGTCAAAC TCCGCTATGG AAAAGGACCT 
GAGGATTGCC CCTACCTGTG GGAGCACATG CAGAAATACA CTGAAATTAC TGCCAAATAT 
TTCCACGGAG TCCGATTGGA CAACTGCCAC TCTACTCCCT TGCATGTTGC TGAGTTCATG 
TTGGATGTGG CCAGAAGCCA GTGTCCTAGC CTGTATGTGG TGGCTGAACT GTTCACTGGC 
AGCGAGGAGC TGGATAACAT CTTTGTTACC AGACTAGGGA TTACATCCCT CATACGAGAG 
GCCATGTCCG CCGGAGACAG CCATGAAGAG GGCCGGCTGG TCTATCGTTA CGGTGGAGAG 
CCAGTCGGAG CCTTCGTTCA GCCCAGCCTG CGTCCTCTCA TGCCTTCTAT CGCTCACGCC 
ATGTTCTTGG ATGTGACCCA TGACAATGAG TGTCCCATTC AGCTGCGGTC AGCTCTCGAC 
TCGTTGCCCA GTTCTGCTAT CGTTTCCATG GCCTGCTGTG CTACTGGCTC CACCAGAGGA 
TATGATGAAC TGGTCCCACA TCAGATATCT GTGGTGAAGG AGGAGCGTTT TTATCCCAAG 
TGGAATCCCT CTTCAGCACC CATCTCTACT GGTGAAGTTG GCTTTCAGAG CGGCATCATC 
GCTGGGAAAC TGGCTCTCAA CAAGCTACAC CAGGAGCTGG CTGCCCAGGG CTTTTCACAG 
GTGTATGTGG ACCAGGTTGA TACAGATATT GTTGCAGTTA CTCGTCACTG CCCCAGCACA 
CACCAGTCTG TGGTAACAGT GTGTAGGACA GCCTTCAAGA ACCCCAAAAC CCATCAGTAC 
AGCAACAATG TCCCACCTAT GTTCATACCT GGCAAGATTG AAGAAATTGT ACTGGAGGCA 
AGGACAGTGG AGAGAGAGGC TGGGAGTTAC AAGAAGGATG AGAAATACAT AAATGGCATG 
CCAGAATACA CAGTGGAAAT AAAAGAGCAT GTACCGATGG TGAAAAGTGC AGTGGTGAAA 
CATGCTGGAG TGACCTCTAA AGACCGCTCA GAGTTTGTGC AAGAAATTCA ATTCCAGAAA 
TTGACTCCTG GTAGCATCAT TGCCTTCAGA GTCTGTTTGG ATCCCAAAGC ACAGAAGCTG 
GTGGGCATGT TGAGGTTTTA TCTGTCCCAG TTCAGTCCAA AGTACAGGAG GGGCAGTGTA 
CCTGATGAGA ATCCACAAGA GGTGCTGCAG AAACCCCTTG CACAGTTAAT GTCTAAGCTG 
ACGCTAGCCG ACATGAACAT CTTGCTGTTT CGCTGCGAAG CAGAAGAACA AGAGGACGGT 
GGAGGCTGTT ACAGCATCCC AGGCTGGGAG GGCCTGAAGT ATTCAGGACT ACAAGGTCTG 
ATATCTGTGT TGGCGGACAT CCGTCCCACT AATGACCTTG GCCATCCTCT GTGTGCTAAC 
CTCAGAGAAG GAGACTGGCT CATAGACTTC GTCTCCAACA GGCTGAAGCA CAGAGATGGA 
CCACTGGCAC AGGTGGGCCA GTGGTTGGGT GCCATGTTTG ACTACCTGAG GCACTTCCCT 
CGATACCTCA TCCCTTGTTA CTTTGACGCC ATCTTGGTGT CAACCTACAC CACAGCTCTG 
GATGCCACGT TCAAACTCAT GTCGAGCTTC ATTCAGCGAG GTTCCACGTT TGTCCGTCAC 
CTGGCTCTCG GCTCAGTTCA GATGTGTGGA GTTGGACGTT TTCCACCTCT GCCTCCCATT 
TCAACAAAAG TGCAGGATGT CCCATACCGC ATCAGTCCCA TCACTGGGGA AAAGGAGCAG 
TGCTGTGTCT CACTGGCAGC AGGTCTTCCT CATTTTTCTG CTGGGATTTT CCGTTGTTGG 
GGTAGAGACA CTTTCATCGC TCTCAGAGGA CTCATGCTTA TAACAGGGAG ACACACTGAG 
GCACGCAACA TCATCCTTGC TTTTGCTGGA ACCCTGCGTC ACGGTTTGAT CCCCAACTTG 
ATGGGAGAGG GTCGCAATGC CAGATACAAC TGCAGGGATG CTGTGTGGTG GTGGCTGCAG 
TGCATTCAGG ACTATACCAA TCTCGTTCCT GAGGGACATG AAATTCTGCG TTGCCCTGTC 
ACCCGCATGT ACCCCACAGA CGACTGTGAG CCCTGCAAAC CAGGGGAGGT GGAGCAGCCA 
TTGTATGATG TTATCCAGGA GGCACTGCAG CGCCATCTGG AGGGTATTTC CTTCAGAGAG 
AGGAATGCTG GACCCAAGAT GGACAGGAAC ATGAGAGATG AAGGGTTCAA TGTGGTTGCT 
AAAGTGGACC CAGCAACAGG TTTTGTTACC GGAGGAAACC GCTTCAACTG TGGCACTTGG 
ATGGATAAAA TGGGGGAGAG TGACAGAGCG AGGAACAAGG GGATACCTGC AACTCCAAGA 
GACGGCGCGG CGGTGGAGAT AGTTGGTCTG AGTAAATCAG CAGTTCGTTG GCTTGTGGAG 
CTGCAGTCTA AAGAACTGTT CCCCTATGAT GGCGTTAAGG TGCACAGAGA TGGAAAGGAT 
GTGTTCATCT TGTATACTGA GTGGAACAAG CAGCTTCAGG AGTCCTTTGA AGTTGAATTC 
TGGGTGTCTG GGGACTCACA GGACCCCAAT GAGAAGCACC CTGATTTGGT GCATAAAAAG 
GGAATTTATA AAGATAGTTA TGGAGCTTCC AATCCCTGGT GTGACTACCA GCTGAGGCCC 
AACTTTACCA TCGCAATGGT GGTGGCTCCA GAGCTGTTCA CGCTAGAAAG AGCATGGAAG 
TCTTTGGAAA TAGCTGAAAA GAAACTCCTG GGACCACTGG GAATGAAAAC TCTTGACCCA 
GATGACATGG TCTACTGTGG TGTTTACGAT AATGCACTGG ACAACGACAA CTACAACCTG 
GCCAAGGGCT TTAACTACCA CCAAGGACCT GAGTGGCTGT GGCCTGTTGG CTATTTTCTC 
CGTGCTAAGC TCTACTTTGC CAAGAGGTTA GGGGAGGAGA CCTACTCCAA GACCGTCATC 
CTAGTGAAAA ATGTTCTGTC ACGACATTGC ACCCACTTGG AGAGGTCTCT ATGGAAAGGC 
TTACCAGAGC TGACCAATGA AAACGGCCAG CATTGCCCCT TCAGCTGTGA GACCCAGGCC 
TGGTCTCTGG CTACTGTGCT GGAGGTGCTC CATGATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008431722.1
CDS	198..4919
Translation	MLLLTGFFFSLRLLIVICLMPVSLETRVPSKFRRTSMMLPQHLKQIRVLMLNEKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYADVERLGGGYVVVDPILRVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSYTWEDVGRLVEKLRTEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNESHLNALRRALWEDIFPQIKLWEFYQVNVENAVEKFRVLLQNGTKPDHSKAEGNKGIEIIQDLQCYRYGNRVNMDSALETFVPHSSSPQHIDECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCVVGNIVYERLAGHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWEHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDVARSQCPSLYVVAELFTGSEELDNIFVTRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIQLRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPISTGEVGFQSGIIAGKLALNKLHQELAAQGFSQVYVDQVDTDIVAVTRHCPSTHQSVVTVCRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREAGSYKKDEKYINGMPEYTVEIKEHVPMVKSAVVKHAGVTSKDRSEFVQEIQFQKLTPGSIIAFRVCLDPKAQKLVGMLRFYLSQFSPKYRRGSVPDENPQEVLQKPLAQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWEGLKYSGLQGLISVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAMFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIQRGSTFVRHLALGSVQMCGVGRFPPLPPISTKVQDVPYRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLMGEGRNARYNCRDAVWWWLQCIQDYTNLVPEGHEILRCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKMDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGIPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVHRDGKDVFILYTEWNKQLQESFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKSLEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKRLGEETYSKTVILVKNVLSRHCTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_008433500.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008433500.2

ORF Insert Sequence:

ATGCTGCTAC TTACAGGTTT CTTCTTCAGT TTGAGACTCC TCATTGTAAT CTGTCTAATG 
CCCGTCTCTT TGGAGACCAG GGTTCCCTCC AAGTTCAGGA GAACTTCCAT GATGCTGCCT 
CAGCACCTAA AGCAAATCAG AGTACTCATG CTTAACGAAA AGGAGAATTT AGAGAGAACT 
TTGTTCAGAC TTGAACAAGG TTTTGAGCTC CAGTTCCGCC TGGGTCCAAG CCTTCAAGGA 
AGGAGAGTTA TAGTTCACAC CAACTACCCA CTTGATGGAC AGAAGTTCAT TCGAAACAAC 
TTCCGTGTTT TAGCCTGGAA CTACCCAACA GGAAGGGAGG ATGACTCTGA TAAGTACTGC 
TCCCTAGAGC TCAAGATTGC TGGATCCTAC CAGTACTACT TTGGATATGC AGATGTGGAG 
AGGTTAGGAG GAGGCTACGT CGTCGTTGAT CCTATTCTTC GTGTTGGAGC AGACAACCAT 
GTCCTCCTGC TGGACTGTGT CACCATCCAG ACATACTTGT CCAAATGTCT GGGACGCCTT 
GATGACTGGC CAGACAGACT GCGAGTAGCC AAGGAGTCAG GTTACAACAT GATCCACTTC 
ACTCCTCTGC AGACTTTGGG AGAGTCTAGG TCCTGTTACT CCTTAGCTGA CCAGCTGACC 
TTTAGCCTGG AATTCAGCCC AGATGGCAAG AGTTACACCT GGGAAGATGT GGGAAGGCTA 
GTGGAAAAAC TGAGGACGGA ATGGAATATG GTATCAATCA CTGATGTAGT GTATAATCAC 
ACTGCTGCAA ACAGTGTGTG GATAAAGGAA CATCCAGAGT GTGGCTACAA CCTGATGAAC 
TCTCCTCACC TGCGTCCTGC CTGGGTCTTA GACAGAGCTA TCTGGCATTT AACCACTAGG 
GTAGCAGATG GACGGTACAA AGAGAAGGGA CTTCCTTCTG ACATTACCAA CGAGAGCCAC 
CTAAATGCTC TCCGCAGGGC GCTTTGGGAG GACATTTTTC CTCAAATCAA ACTTTGGGAG 
TTCTACCAGG TCAACGTGGA AAATGCTGTG GAGAAGTTCA GAGTACTGCT GCAAAACGGC 
ACAAAGCCAG ATCATAGCAA GGCTGAAGGA AATAAAGGCA TAGAGATCAT TCAAGACCTC 
CAGTGCTATC GCTATGGCAA CAGAGTGAAC ATGGACTCAG CTTTGGAGAC ATTTGTTCCT 
CACAGCTCCT CTCCACAGCA CATTGATGAG TGCTGTGGTT GGCTGAAACA GAAGTTGACT 
GAGATGAATG AAGAACAGTA CCACATCATA CACCAGCATC AGGAACAGGC CGTCAACTGC 
GTTGTGGGGA ACATAGTGTA TGAACGTCTA GCTGGCCACG GTCCTAAACT GGGTCCTGTT 
ACCAGGAAAG AGCCTCTTGT CACCAGGTAT TTCACCTTCC CCTTTGAGGA AATGACTTTA 
GAGCAGGAGA TGGAGTTTTT AAACCAACCA GATAAGATGT GCCACTTCTT GGCTCACAAC 
GGCTGGGTGA TGGGTGATGA TCCATTGCGC AACTTTGCTG AACCAGGATC CAATGTGTAC 
CTGCGTCGGG AGCTGATCTG CTGGGGGGAC AGTGTCAAAC TCCGCTATGG AAAAGGACCT 
GAGGATTGCC CCTACCTGTG GGAGCACATG CAGAAATACA CTGAAATTAC TGCCAAATAT 
TTCCACGGAG TCCGATTGGA CAACTGCCAC TCTACTCCCT TGCATGTTGC TGAGTTCATG 
TTGGATGTGG CCAGAAGCCA GTGTCCTAGC CTGTATGTGG TGGCTGAACT GTTCACTGGC 
AGCGAGGAGC TGGATAACAT CTTTGTTACC AGACTAGGGA TTACATCCCT CATACGAGAG 
GCCATGTCCG CCGGAGACAG CCATGAAGAG GGCCGGCTGG TCTATCGTTA CGGTGGAGAG 
CCAGTCGGAG CCTTCGTTCA GCCCAGCCTG CGTCCTCTCA TGCCTTCTAT CGCTCACGCC 
ATGTTCTTGG ATGTGACCCA TGACAATGAG TGTCCCATTC AGCTGCGGTC AGCTCTCGAC 
TCGTTGCCCA GTTCTGCTAT CGTTTCCATG GCCTGCTGTG CTACTGGCTC CACCAGAGGA 
TATGATGAAC TGGTCCCACA TCAGATATCT GTGGTGAAGG AGGAGCGTTT TTATCCCAAG 
TGGAATCCCT CTTCAGCACC CATCTCTACT GGTGAAGTTG GCTTTCAGAG CGGCATCATC 
GCTGGGAAAC TGGCTCTCAA CAAGCTACAC CAGGAGCTGG CTGCCCAGGG CTTTTCACAG 
GTGTATGTGG ACCAGGTTGA TACAGATATT GTTGCAGTTA CTCGTCACTG CCCCAGCACA 
CACCAGTCTG TGGTAACAGT GTGTAGGACA GCCTTCAAGA ACCCCAAAAC CCATCAGTAC 
AGCAACAATG TCCCACCTAT GTTCATACCT GGCAAGATTG AAGAAATTGT ACTGGAGGCA 
AGGACAGTGG AGAGAGAGGC TGGGAGTTAC AAGAAGGATG AGAAATACAT AAATGGCATG 
CCAGAATACA CAGTGGAAAT AAAAGAGCAT GTACCGATGG TGAAAAGTGC AGTGGTGAAA 
CATGCTGGAG TGACCTCTAA AGACCGCTCA GAGTTTGTGC AAGAAATTCA ATTCCAGAAA 
TTGACTCCTG GTAGCATCAT TGCCTTCAGA GTCTGTTTGG ATCCCAAAGC ACAGAAGCTG 
GTGGGCATGT TGAGGTTTTA TCTGTCCCAG TTCAGTCCAA AGTACAGGAG GGGCAGTGTA 
CCTGATGAGA ATCCACAAGA GGTGCTGCAG AAACCCCTTG CACAGTTAAT GTCTAAGCTG 
ACGCTAGCCG ACATGAACAT CTTGCTGTTT CGCTGCGAAG CAGAAGAACA AGAGGACGGT 
GGAGGCTGTT ACAGCATCCC AGGCTGGGAG GGCCTGAAGT ATTCAGGACT ACAAGGTCTG 
ATATCTGTGT TGGCGGACAT CCGTCCCACT AATGACCTTG GCCATCCTCT GTGTGCTAAC 
CTCAGAGAAG GAGACTGGCT CATAGACTTC GTCTCCAACA GGCTGAAGCA CAGAGATGGA 
CCACTGGCAC AGGTGGGCCA GTGGTTGGGT GCCATGTTTG ACTACCTGAG GCACTTCCCT 
CGATACCTCA TCCCTTGTTA CTTTGACGCC ATCTTGGTGT CAACCTACAC CACAGCTCTG 
GATGCCACGT TCAAACTCAT GTCGAGCTTC ATTCAGCGAG GTTCCACGTT TGTCCGTCAC 
CTGGCTCTCG GCTCAGTTCA GATGTGTGGA GTTGGACGTT TTCCACCTCT GCCTCCCATT 
TCAACAAAAG TGCAGGATGT CCCATACCGC ATCAGTCCCA TCACTGGGGA AAAGGAGCAG 
TGCTGTGTCT CACTGGCAGC AGGTCTTCCT CATTTTTCTG CTGGGATTTT CCGTTGTTGG 
GGTAGAGACA CTTTCATCGC TCTCAGAGGA CTCATGCTTA TAACAGGGAG ACACACTGAG 
GCACGCAACA TCATCCTTGC TTTTGCTGGA ACCCTGCGTC ACGGTTTGAT CCCCAACTTG 
ATGGGAGAGG GTCGCAATGC CAGATACAAC TGCAGGGATG CTGTGTGGTG GTGGCTGCAG 
TGCATTCAGG ACTATACCAA TCTCGTTCCT GAGGGACATG AAATTCTGCG TTGCCCTGTC 
ACCCGCATGT ACCCCACAGA CGACTGTGAG CCCTGCAAAC CAGGGGAGGT GGAGCAGCCA 
TTGTATGATG TTATCCAGGA GGCACTGCAG CGCCATCTGG AGGGTATTTC CTTCAGAGAG 
AGGAATGCTG GACCCAAGAT GGACAGGAAC ATGAGAGATG AAGGGTTCAA TGTGGTTGCT 
AAAGTGGACC CAGCAACAGG TTTTGTTACC GGAGGAAACC GCTTCAACTG TGGCACTTGG 
ATGGATAAAA TGGGGGAGAG TGACAGAGCG AGGAACAAGG GGATACCTGC AACTCCAAGA 
GACGGCGCGG CGGTGGAGAT AGTTGGTCTG AGTAAATCAG CAGTTCGTTG GCTTGTGGAG 
CTGCAGTCTA AAGAACTGTT CCCCTATGAT GGCGTTAAGG TGCACAGAGA TGGAAAGGAT 
GTGTTCATCT TGTATACTGA GTGGAACAAG CAGCTTCAGG AGTCCTTTGA AGTTGAATTC 
TGGGTGTCTG GGGACTCACA GGACCCCAAT GAGAAGCACC CTGATTTGGT GCATAAAAAG 
GGAATTTATA AAGATAGTTA TGGAGCTTCC AATCCCTGGT GTGACTACCA GCTGAGGCCC 
AACTTTACCA TCGCAATGGT GGTGGCTCCA GAGCTGTTCA CGCTAGAAAG AGCATGGAAG 
TCTTTGGAAA TAGCTGAAAA GAAACTCCTG GGACCACTGG GAATGAAAAC TCTTGACCCA 
GATGACATGG TCTACTGTGG TGTTTACGAT AATGCACTGG ACAACGACAA CTACAACCTG 
GCCAAGGGCT TTAACTACCA CCAAGGACCT GAGTGGCTGT GGCCTGTTGG CTATTTTCTC 
CGTGCTAAGC TCTACTTTGC CAAGAGGTTA GGGGAGGAGA CCTACTCCAA GACCGTCATC 
CTAGTGAAAA ATGTTCTGTC ACGACATTGC ACCCACTTGG AGAGGTCTCT ATGGAAAGGC 
TTACCAGAGC TGACCAATGA AAACGGCCAG CATTGCCCCT TCAGCTGTGA GACCCAGGCC 
TGGTCTCTGG CTACTGTGCT GGAGGTGCTC CATGATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_017309975.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017165464.1 Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA.

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CloneID	OPo179979
Clone ID Related Accession (Same CDS sequence)	XM_017309975.1 , XM_008433502.2
Accession Version	XM_017309975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	glycogen debranching enzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGC CTCAGCACCT AAAGCAAATC AGAGTACTCA TGCTTAACGA AAAGGAGAAT 
TTAGAGAGAA CTTTGTTCAG ACTTGAACAA GGTTTTGAGC TCCAGTTCCG CCTGGGTCCA 
AGCCTTCAAG GAAGGAGAGT TATAGTTCAC ACCAACTACC CACTTGATGG ACAGAAGTTC 
ATTCGAAACA ACTTCCGTGT TTTAGCCTGG AACTACCCAA CAGGAAGGGA GGATGACTCT 
GATAAGTACT GCTCCCTAGA GCTCAAGATT GCTGGATCCT ACCAGTACTA CTTTGGATAT 
GCAGATGTGG AGAGGTTAGG AGGAGGCTAC GTCGTCGTTG ATCCTATTCT TCGTGTTGGA 
GCAGACAACC ATGTCCTCCT GCTGGACTGT GTCACCATCC AGACATACTT GTCCAAATGT 
CTGGGACGCC TTGATGACTG GCCAGACAGA CTGCGAGTAG CCAAGGAGTC AGGTTACAAC 
ATGATCCACT TCACTCCTCT GCAGACTTTG GGAGAGTCTA GGTCCTGTTA CTCCTTAGCT 
GACCAGCTGA CCTTTAGCCT GGAATTCAGC CCAGATGGCA AGAGTTACAC CTGGGAAGAT 
GTGGGAAGGC TAGTGGAAAA ACTGAGGACG GAATGGAATA TGGTATCAAT CACTGATGTA 
GTGTATAATC ACACTGCTGC AAACAGTGTG TGGATAAAGG AACATCCAGA GTGTGGCTAC 
AACCTGATGA ACTCTCCTCA CCTGCGTCCT GCCTGGGTCT TAGACAGAGC TATCTGGCAT 
TTAACCACTA GGGTAGCAGA TGGACGGTAC AAAGAGAAGG GACTTCCTTC TGACATTACC 
AACGAGAGCC ACCTAAATGC TCTCCGCAGG GCGCTTTGGG AGGACATTTT TCCTCAAATC 
AAACTTTGGG AGTTCTACCA GGTCAACGTG GAAAATGCTG TGGAGAAGTT CAGAGTACTG 
CTGCAAAACG GCACAAAGCC AGATCATAGC AAGGCTGAAG GAAATAAAGG CATAGAGATC 
ATTCAAGACC TCCAGTGCTA TCGCTATGGC AACAGAGTGA ACATGGACTC AGCTTTGGAG 
ACATTTGTTC CTCACAGCTC CTCTCCACAG CACATTGATG AGTGCTGTGG TTGGCTGAAA 
CAGAAGTTGA CTGAGATGAA TGAAGAACAG TACCACATCA TACACCAGCA TCAGGAACAG 
GCCGTCAACT GCGTTGTGGG GAACATAGTG TATGAACGTC TAGCTGGCCA CGGTCCTAAA 
CTGGGTCCTG TTACCAGGAA AGAGCCTCTT GTCACCAGGT ATTTCACCTT CCCCTTTGAG 
GAAATGACTT TAGAGCAGGA GATGGAGTTT TTAAACCAAC CAGATAAGAT GTGCCACTTC 
TTGGCTCACA ACGGCTGGGT GATGGGTGAT GATCCATTGC GCAACTTTGC TGAACCAGGA 
TCCAATGTGT ACCTGCGTCG GGAGCTGATC TGCTGGGGGG ACAGTGTCAA ACTCCGCTAT 
GGAAAAGGAC CTGAGGATTG CCCCTACCTG TGGGAGCACA TGCAGAAATA CACTGAAATT 
ACTGCCAAAT ATTTCCACGG AGTCCGATTG GACAACTGCC ACTCTACTCC CTTGCATGTT 
GCTGAGTTCA TGTTAGATGC AGCCAGAGAG GTCAGACCCA ACCTGTATGT AATAGCTGAG 
CTCTTTACAG GCAGTGAACT CATAGACAAT GTGTTTGTTA ACCGACTGGG AATCAACAGC 
CTCATAAGAG AGGCCATGTC CGCCGGAGAC AGCCATGAAG AGGGCCGGCT GGTCTATCGT 
TACGGTGGAG AGCCAGTCGG AGCCTTCGTT CAGCCCAGCC TGCGTCCTCT CATGCCTTCT 
ATCGCTCACG CCATGTTCTT GGATGTGACC CATGACAATG AGTGTCCCAT TCAGCTGCGG 
TCAGCTCTCG ACTCGTTGCC CAGTTCTGCT ATCGTTTCCA TGGCCTGCTG TGCTACTGGC 
TCCACCAGAG GATATGATGA ACTGGTCCCA CATCAGATAT CTGTGGTGAA GGAGGAGCGT 
TTTTATCCCA AGTGGAATCC CTCTTCAGCA CCCATCTCTA CTGGTGAAGT TGGCTTTCAG 
AGCGGCATCA TCGCTGGGAA ACTGGCTCTC AACAAGCTAC ACCAGGAGCT GGCTGCCCAG 
GGCTTTTCAC AGGTGTATGT GGACCAGGTT GATACAGATA TTGTTGCAGT TACTCGTCAC 
TGCCCCAGCA CACACCAGTC TGTGGTAACA GTGTGTAGGA CAGCCTTCAA GAACCCCAAA 
ACCCATCAGT ACAGCAACAA TGTCCCACCT ATGTTCATAC CTGGCAAGAT TGAAGAAATT 
GTACTGGAGG CAAGGACAGT GGAGAGAGAG GCTGGGAGTT ACAAGAAGGA TGAGAAATAC 
ATAAATGGCA TGCCAGAATA CACAGTGGAA ATAAAAGAGC ATGTACCGAT GGTGAAAAGT 
GCAGTGGTGA AACATGCTGG AGTGACCTCT AAAGACCGCT CAGAGTTTGT GCAAGAAATT 
CAATTCCAGA AATTGACTCC TGGTAGCATC ATTGCCTTCA GAGTCTGTTT GGATCCCAAA 
GCACAGAAGC TGGTGGGCAT GTTGAGGTTT TATCTGTCCC AGTTCAGTCC AAAGTACAGG 
AGGGGCAGTG TACCTGATGA GAATCCACAA GAGGTGCTGC AGAAACCCCT TGCACAGTTA 
ATGTCTAAGC TGACGCTAGC CGACATGAAC ATCTTGCTGT TTCGCTGCGA AGCAGAAGAA 
CAAGAGGACG GTGGAGGCTG TTACAGCATC CCAGGCTGGG AGGGCCTGAA GTATTCAGGA 
CTACAAGGTC TGATATCTGT GTTGGCGGAC ATCCGTCCCA CTAATGACCT TGGCCATCCT 
CTGTGTGCTA ACCTCAGAGA AGGAGACTGG CTCATAGACT TCGTCTCCAA CAGGCTGAAG 
CACAGAGATG GACCACTGGC ACAGGTGGGC CAGTGGTTGG GTGCCATGTT TGACTACCTG 
AGGCACTTCC CTCGATACCT CATCCCTTGT TACTTTGACG CCATCTTGGT GTCAACCTAC 
ACCACAGCTC TGGATGCCAC GTTCAAACTC ATGTCGAGCT TCATTCAGCG AGGTTCCACG 
TTTGTCCGTC ACCTGGCTCT CGGCTCAGTT CAGATGTGTG GAGTTGGACG TTTTCCACCT 
CTGCCTCCCA TTTCAACAAA AGTGCAGGAT GTCCCATACC GCATCAGTCC CATCACTGGG 
GAAAAGGAGC AGTGCTGTGT CTCACTGGCA GCAGGTCTTC CTCATTTTTC TGCTGGGATT 
TTCCGTTGTT GGGGTAGAGA CACTTTCATC GCTCTCAGAG GACTCATGCT TATAACAGGG 
AGACACACTG AGGCACGCAA CATCATCCTT GCTTTTGCTG GAACCCTGCG TCACGGTTTG 
ATCCCCAACT TGATGGGAGA GGGTCGCAAT GCCAGATACA ACTGCAGGGA TGCTGTGTGG 
TGGTGGCTGC AGTGCATTCA GGACTATACC AATCTCGTTC CTGAGGGACA TGAAATTCTG 
CGTTGCCCTG TCACCCGCAT GTACCCCACA GACGACTGTG AGCCCTGCAA ACCAGGGGAG 
GTGGAGCAGC CATTGTATGA TGTTATCCAG GAGGCACTGC AGCGCCATCT GGAGGGTATT 
TCCTTCAGAG AGAGGAATGC TGGACCCAAG ATGGACAGGA ACATGAGAGA TGAAGGGTTC 
AATGTGGTTG CTAAAGTGGA CCCAGCAACA GGTTTTGTTA CCGGAGGAAA CCGCTTCAAC 
TGTGGCACTT GGATGGATAA AATGGGGGAG AGTGACAGAG CGAGGAACAA GGGGATACCT 
GCAACTCCAA GAGACGGCGC GGCGGTGGAG ATAGTTGGTC TGAGTAAATC AGCAGTTCGT 
TGGCTTGTGG AGCTGCAGTC TAAAGAACTG TTCCCCTATG ATGGCGTTAA GGTGCACAGA 
GATGGAAAGG ATGTGTTCAT CTTGTATACT GAGTGGAACA AGCAGCTTCA GGAGTCCTTT 
GAAGTTGAAT TCTGGGTGTC TGGGGACTCA CAGGACCCCA ATGAGAAGCA CCCTGATTTG 
GTGCATAAAA AGGGAATTTA TAAAGATAGT TATGGAGCTT CCAATCCCTG GTGTGACTAC 
CAGCTGAGGC CCAACTTTAC CATCGCAATG GTGGTGGCTC CAGAGCTGTT CACGCTAGAA 
AGAGCATGGA AGTCTTTGGA AATAGCTGAA AAGAAACTCC TGGGACCACT GGGAATGAAA 
ACTCTTGACC CAGATGACAT GGTCTACTGT GGTGTTTACG ATAATGCACT GGACAACGAC 
AACTACAACC TGGCCAAGGG CTTTAACTAC CACCAAGGAC CTGAGTGGCT GTGGCCTGTT 
GGCTATTTTC TCCGTGCTAA GCTCTACTTT GCCAAGAGGT TAGGGGAGGA GACCTACTCC 
AAGACCGTCA TCCTAGTGAA AAATGTTCTG TCACGACATT GCACCCACTT GGAGAGGTCT 
CTATGGAAAG GCTTACCAGA GCTGACCAAT GAAAACGGCC AGCATTGCCC CTTCAGCTGT 
GAGACCCAGG CCTGGTCTCT GGCTACTGTG CTGGAGGTGC TCCATGATCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017165464.1
CDS	142..4755
Translation	MMLPQHLKQIRVLMLNEKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYADVERLGGGYVVVDPILRVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSYTWEDVGRLVEKLRTEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNESHLNALRRALWEDIFPQIKLWEFYQVNVENAVEKFRVLLQNGTKPDHSKAEGNKGIEIIQDLQCYRYGNRVNMDSALETFVPHSSSPQHIDECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCVVGNIVYERLAGHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWEHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDAAREVRPNLYVIAELFTGSELIDNVFVNRLGINSLIREAMSAGDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIQLRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPISTGEVGFQSGIIAGKLALNKLHQELAAQGFSQVYVDQVDTDIVAVTRHCPSTHQSVVTVCRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREAGSYKKDEKYINGMPEYTVEIKEHVPMVKSAVVKHAGVTSKDRSEFVQEIQFQKLTPGSIIAFRVCLDPKAQKLVGMLRFYLSQFSPKYRRGSVPDENPQEVLQKPLAQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWEGLKYSGLQGLISVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAMFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIQRGSTFVRHLALGSVQMCGVGRFPPLPPISTKVQDVPYRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLMGEGRNARYNCRDAVWWWLQCIQDYTNLVPEGHEILRCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKMDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGIPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVHRDGKDVFILYTEWNKQLQESFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKSLEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKRLGEETYSKTVILVKNVLSRHCTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_017309975.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017309975.1

ORF Insert Sequence:

ATGATGCTGC CTCAGCACCT AAAGCAAATC AGAGTACTCA TGCTTAACGA AAAGGAGAAT 
TTAGAGAGAA CTTTGTTCAG ACTTGAACAA GGTTTTGAGC TCCAGTTCCG CCTGGGTCCA 
AGCCTTCAAG GAAGGAGAGT TATAGTTCAC ACCAACTACC CACTTGATGG ACAGAAGTTC 
ATTCGAAACA ACTTCCGTGT TTTAGCCTGG AACTACCCAA CAGGAAGGGA GGATGACTCT 
GATAAGTACT GCTCCCTAGA GCTCAAGATT GCTGGATCCT ACCAGTACTA CTTTGGATAT 
GCAGATGTGG AGAGGTTAGG AGGAGGCTAC GTCGTCGTTG ATCCTATTCT TCGTGTTGGA 
GCAGACAACC ATGTCCTCCT GCTGGACTGT GTCACCATCC AGACATACTT GTCCAAATGT 
CTGGGACGCC TTGATGACTG GCCAGACAGA CTGCGAGTAG CCAAGGAGTC AGGTTACAAC 
ATGATCCACT TCACTCCTCT GCAGACTTTG GGAGAGTCTA GGTCCTGTTA CTCCTTAGCT 
GACCAGCTGA CCTTTAGCCT GGAATTCAGC CCAGATGGCA AGAGTTACAC CTGGGAAGAT 
GTGGGAAGGC TAGTGGAAAA ACTGAGGACG GAATGGAATA TGGTATCAAT CACTGATGTA 
GTGTATAATC ACACTGCTGC AAACAGTGTG TGGATAAAGG AACATCCAGA GTGTGGCTAC 
AACCTGATGA ACTCTCCTCA CCTGCGTCCT GCCTGGGTCT TAGACAGAGC TATCTGGCAT 
TTAACCACTA GGGTAGCAGA TGGACGGTAC AAAGAGAAGG GACTTCCTTC TGACATTACC 
AACGAGAGCC ACCTAAATGC TCTCCGCAGG GCGCTTTGGG AGGACATTTT TCCTCAAATC 
AAACTTTGGG AGTTCTACCA GGTCAACGTG GAAAATGCTG TGGAGAAGTT CAGAGTACTG 
CTGCAAAACG GCACAAAGCC AGATCATAGC AAGGCTGAAG GAAATAAAGG CATAGAGATC 
ATTCAAGACC TCCAGTGCTA TCGCTATGGC AACAGAGTGA ACATGGACTC AGCTTTGGAG 
ACATTTGTTC CTCACAGCTC CTCTCCACAG CACATTGATG AGTGCTGTGG TTGGCTGAAA 
CAGAAGTTGA CTGAGATGAA TGAAGAACAG TACCACATCA TACACCAGCA TCAGGAACAG 
GCCGTCAACT GCGTTGTGGG GAACATAGTG TATGAACGTC TAGCTGGCCA CGGTCCTAAA 
CTGGGTCCTG TTACCAGGAA AGAGCCTCTT GTCACCAGGT ATTTCACCTT CCCCTTTGAG 
GAAATGACTT TAGAGCAGGA GATGGAGTTT TTAAACCAAC CAGATAAGAT GTGCCACTTC 
TTGGCTCACA ACGGCTGGGT GATGGGTGAT GATCCATTGC GCAACTTTGC TGAACCAGGA 
TCCAATGTGT ACCTGCGTCG GGAGCTGATC TGCTGGGGGG ACAGTGTCAA ACTCCGCTAT 
GGAAAAGGAC CTGAGGATTG CCCCTACCTG TGGGAGCACA TGCAGAAATA CACTGAAATT 
ACTGCCAAAT ATTTCCACGG AGTCCGATTG GACAACTGCC ACTCTACTCC CTTGCATGTT 
GCTGAGTTCA TGTTAGATGC AGCCAGAGAG GTCAGACCCA ACCTGTATGT AATAGCTGAG 
CTCTTTACAG GCAGTGAACT CATAGACAAT GTGTTTGTTA ACCGACTGGG AATCAACAGC 
CTCATAAGAG AGGCCATGTC CGCCGGAGAC AGCCATGAAG AGGGCCGGCT GGTCTATCGT 
TACGGTGGAG AGCCAGTCGG AGCCTTCGTT CAGCCCAGCC TGCGTCCTCT CATGCCTTCT 
ATCGCTCACG CCATGTTCTT GGATGTGACC CATGACAATG AGTGTCCCAT TCAGCTGCGG 
TCAGCTCTCG ACTCGTTGCC CAGTTCTGCT ATCGTTTCCA TGGCCTGCTG TGCTACTGGC 
TCCACCAGAG GATATGATGA ACTGGTCCCA CATCAGATAT CTGTGGTGAA GGAGGAGCGT 
TTTTATCCCA AGTGGAATCC CTCTTCAGCA CCCATCTCTA CTGGTGAAGT TGGCTTTCAG 
AGCGGCATCA TCGCTGGGAA ACTGGCTCTC AACAAGCTAC ACCAGGAGCT GGCTGCCCAG 
GGCTTTTCAC AGGTGTATGT GGACCAGGTT GATACAGATA TTGTTGCAGT TACTCGTCAC 
TGCCCCAGCA CACACCAGTC TGTGGTAACA GTGTGTAGGA CAGCCTTCAA GAACCCCAAA 
ACCCATCAGT ACAGCAACAA TGTCCCACCT ATGTTCATAC CTGGCAAGAT TGAAGAAATT 
GTACTGGAGG CAAGGACAGT GGAGAGAGAG GCTGGGAGTT ACAAGAAGGA TGAGAAATAC 
ATAAATGGCA TGCCAGAATA CACAGTGGAA ATAAAAGAGC ATGTACCGAT GGTGAAAAGT 
GCAGTGGTGA AACATGCTGG AGTGACCTCT AAAGACCGCT CAGAGTTTGT GCAAGAAATT 
CAATTCCAGA AATTGACTCC TGGTAGCATC ATTGCCTTCA GAGTCTGTTT GGATCCCAAA 
GCACAGAAGC TGGTGGGCAT GTTGAGGTTT TATCTGTCCC AGTTCAGTCC AAAGTACAGG 
AGGGGCAGTG TACCTGATGA GAATCCACAA GAGGTGCTGC AGAAACCCCT TGCACAGTTA 
ATGTCTAAGC TGACGCTAGC CGACATGAAC ATCTTGCTGT TTCGCTGCGA AGCAGAAGAA 
CAAGAGGACG GTGGAGGCTG TTACAGCATC CCAGGCTGGG AGGGCCTGAA GTATTCAGGA 
CTACAAGGTC TGATATCTGT GTTGGCGGAC ATCCGTCCCA CTAATGACCT TGGCCATCCT 
CTGTGTGCTA ACCTCAGAGA AGGAGACTGG CTCATAGACT TCGTCTCCAA CAGGCTGAAG 
CACAGAGATG GACCACTGGC ACAGGTGGGC CAGTGGTTGG GTGCCATGTT TGACTACCTG 
AGGCACTTCC CTCGATACCT CATCCCTTGT TACTTTGACG CCATCTTGGT GTCAACCTAC 
ACCACAGCTC TGGATGCCAC GTTCAAACTC ATGTCGAGCT TCATTCAGCG AGGTTCCACG 
TTTGTCCGTC ACCTGGCTCT CGGCTCAGTT CAGATGTGTG GAGTTGGACG TTTTCCACCT 
CTGCCTCCCA TTTCAACAAA AGTGCAGGAT GTCCCATACC GCATCAGTCC CATCACTGGG 
GAAAAGGAGC AGTGCTGTGT CTCACTGGCA GCAGGTCTTC CTCATTTTTC TGCTGGGATT 
TTCCGTTGTT GGGGTAGAGA CACTTTCATC GCTCTCAGAG GACTCATGCT TATAACAGGG 
AGACACACTG AGGCACGCAA CATCATCCTT GCTTTTGCTG GAACCCTGCG TCACGGTTTG 
ATCCCCAACT TGATGGGAGA GGGTCGCAAT GCCAGATACA ACTGCAGGGA TGCTGTGTGG 
TGGTGGCTGC AGTGCATTCA GGACTATACC AATCTCGTTC CTGAGGGACA TGAAATTCTG 
CGTTGCCCTG TCACCCGCAT GTACCCCACA GACGACTGTG AGCCCTGCAA ACCAGGGGAG 
GTGGAGCAGC CATTGTATGA TGTTATCCAG GAGGCACTGC AGCGCCATCT GGAGGGTATT 
TCCTTCAGAG AGAGGAATGC TGGACCCAAG ATGGACAGGA ACATGAGAGA TGAAGGGTTC 
AATGTGGTTG CTAAAGTGGA CCCAGCAACA GGTTTTGTTA CCGGAGGAAA CCGCTTCAAC 
TGTGGCACTT GGATGGATAA AATGGGGGAG AGTGACAGAG CGAGGAACAA GGGGATACCT 
GCAACTCCAA GAGACGGCGC GGCGGTGGAG ATAGTTGGTC TGAGTAAATC AGCAGTTCGT 
TGGCTTGTGG AGCTGCAGTC TAAAGAACTG TTCCCCTATG ATGGCGTTAA GGTGCACAGA 
GATGGAAAGG ATGTGTTCAT CTTGTATACT GAGTGGAACA AGCAGCTTCA GGAGTCCTTT 
GAAGTTGAAT TCTGGGTGTC TGGGGACTCA CAGGACCCCA ATGAGAAGCA CCCTGATTTG 
GTGCATAAAA AGGGAATTTA TAAAGATAGT TATGGAGCTT CCAATCCCTG GTGTGACTAC 
CAGCTGAGGC CCAACTTTAC CATCGCAATG GTGGTGGCTC CAGAGCTGTT CACGCTAGAA 
AGAGCATGGA AGTCTTTGGA AATAGCTGAA AAGAAACTCC TGGGACCACT GGGAATGAAA 
ACTCTTGACC CAGATGACAT GGTCTACTGT GGTGTTTACG ATAATGCACT GGACAACGAC 
AACTACAACC TGGCCAAGGG CTTTAACTAC CACCAAGGAC CTGAGTGGCT GTGGCCTGTT 
GGCTATTTTC TCCGTGCTAA GCTCTACTTT GCCAAGAGGT TAGGGGAGGA GACCTACTCC 
AAGACCGTCA TCCTAGTGAA AAATGTTCTG TCACGACATT GCACCCACTT GGAGAGGTCT 
CTATGGAAAG GCTTACCAGA GCTGACCAAT GAAAACGGCC AGCATTGCCC CTTCAGCTGT 
GAGACCCAGG CCTGGTCTCT GGCTACTGTG CTGGAGGTGC TCCATGATCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_008433502.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008431724.1 Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA.

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CloneID	OPo179979
Clone ID Related Accession (Same CDS sequence)	XM_017309975.1 , XM_008433502.2
Accession Version	XM_008433502.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	glycogen debranching enzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008433502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGC CTCAGCACCT AAAGCAAATC AGAGTACTCA TGCTTAACGA AAAGGAGAAT 
TTAGAGAGAA CTTTGTTCAG ACTTGAACAA GGTTTTGAGC TCCAGTTCCG CCTGGGTCCA 
AGCCTTCAAG GAAGGAGAGT TATAGTTCAC ACCAACTACC CACTTGATGG ACAGAAGTTC 
ATTCGAAACA ACTTCCGTGT TTTAGCCTGG AACTACCCAA CAGGAAGGGA GGATGACTCT 
GATAAGTACT GCTCCCTAGA GCTCAAGATT GCTGGATCCT ACCAGTACTA CTTTGGATAT 
GCAGATGTGG AGAGGTTAGG AGGAGGCTAC GTCGTCGTTG ATCCTATTCT TCGTGTTGGA 
GCAGACAACC ATGTCCTCCT GCTGGACTGT GTCACCATCC AGACATACTT GTCCAAATGT 
CTGGGACGCC TTGATGACTG GCCAGACAGA CTGCGAGTAG CCAAGGAGTC AGGTTACAAC 
ATGATCCACT TCACTCCTCT GCAGACTTTG GGAGAGTCTA GGTCCTGTTA CTCCTTAGCT 
GACCAGCTGA CCTTTAGCCT GGAATTCAGC CCAGATGGCA AGAGTTACAC CTGGGAAGAT 
GTGGGAAGGC TAGTGGAAAA ACTGAGGACG GAATGGAATA TGGTATCAAT CACTGATGTA 
GTGTATAATC ACACTGCTGC AAACAGTGTG TGGATAAAGG AACATCCAGA GTGTGGCTAC 
AACCTGATGA ACTCTCCTCA CCTGCGTCCT GCCTGGGTCT TAGACAGAGC TATCTGGCAT 
TTAACCACTA GGGTAGCAGA TGGACGGTAC AAAGAGAAGG GACTTCCTTC TGACATTACC 
AACGAGAGCC ACCTAAATGC TCTCCGCAGG GCGCTTTGGG AGGACATTTT TCCTCAAATC 
AAACTTTGGG AGTTCTACCA GGTCAACGTG GAAAATGCTG TGGAGAAGTT CAGAGTACTG 
CTGCAAAACG GCACAAAGCC AGATCATAGC AAGGCTGAAG GAAATAAAGG CATAGAGATC 
ATTCAAGACC TCCAGTGCTA TCGCTATGGC AACAGAGTGA ACATGGACTC AGCTTTGGAG 
ACATTTGTTC CTCACAGCTC CTCTCCACAG CACATTGATG AGTGCTGTGG TTGGCTGAAA 
CAGAAGTTGA CTGAGATGAA TGAAGAACAG TACCACATCA TACACCAGCA TCAGGAACAG 
GCCGTCAACT GCGTTGTGGG GAACATAGTG TATGAACGTC TAGCTGGCCA CGGTCCTAAA 
CTGGGTCCTG TTACCAGGAA AGAGCCTCTT GTCACCAGGT ATTTCACCTT CCCCTTTGAG 
GAAATGACTT TAGAGCAGGA GATGGAGTTT TTAAACCAAC CAGATAAGAT GTGCCACTTC 
TTGGCTCACA ACGGCTGGGT GATGGGTGAT GATCCATTGC GCAACTTTGC TGAACCAGGA 
TCCAATGTGT ACCTGCGTCG GGAGCTGATC TGCTGGGGGG ACAGTGTCAA ACTCCGCTAT 
GGAAAAGGAC CTGAGGATTG CCCCTACCTG TGGGAGCACA TGCAGAAATA CACTGAAATT 
ACTGCCAAAT ATTTCCACGG AGTCCGATTG GACAACTGCC ACTCTACTCC CTTGCATGTT 
GCTGAGTTCA TGTTAGATGC AGCCAGAGAG GTCAGACCCA ACCTGTATGT AATAGCTGAG 
CTCTTTACAG GCAGTGAACT CATAGACAAT GTGTTTGTTA ACCGACTGGG AATCAACAGC 
CTCATAAGAG AGGCCATGTC CGCCGGAGAC AGCCATGAAG AGGGCCGGCT GGTCTATCGT 
TACGGTGGAG AGCCAGTCGG AGCCTTCGTT CAGCCCAGCC TGCGTCCTCT CATGCCTTCT 
ATCGCTCACG CCATGTTCTT GGATGTGACC CATGACAATG AGTGTCCCAT TCAGCTGCGG 
TCAGCTCTCG ACTCGTTGCC CAGTTCTGCT ATCGTTTCCA TGGCCTGCTG TGCTACTGGC 
TCCACCAGAG GATATGATGA ACTGGTCCCA CATCAGATAT CTGTGGTGAA GGAGGAGCGT 
TTTTATCCCA AGTGGAATCC CTCTTCAGCA CCCATCTCTA CTGGTGAAGT TGGCTTTCAG 
AGCGGCATCA TCGCTGGGAA ACTGGCTCTC AACAAGCTAC ACCAGGAGCT GGCTGCCCAG 
GGCTTTTCAC AGGTGTATGT GGACCAGGTT GATACAGATA TTGTTGCAGT TACTCGTCAC 
TGCCCCAGCA CACACCAGTC TGTGGTAACA GTGTGTAGGA CAGCCTTCAA GAACCCCAAA 
ACCCATCAGT ACAGCAACAA TGTCCCACCT ATGTTCATAC CTGGCAAGAT TGAAGAAATT 
GTACTGGAGG CAAGGACAGT GGAGAGAGAG GCTGGGAGTT ACAAGAAGGA TGAGAAATAC 
ATAAATGGCA TGCCAGAATA CACAGTGGAA ATAAAAGAGC ATGTACCGAT GGTGAAAAGT 
GCAGTGGTGA AACATGCTGG AGTGACCTCT AAAGACCGCT CAGAGTTTGT GCAAGAAATT 
CAATTCCAGA AATTGACTCC TGGTAGCATC ATTGCCTTCA GAGTCTGTTT GGATCCCAAA 
GCACAGAAGC TGGTGGGCAT GTTGAGGTTT TATCTGTCCC AGTTCAGTCC AAAGTACAGG 
AGGGGCAGTG TACCTGATGA GAATCCACAA GAGGTGCTGC AGAAACCCCT TGCACAGTTA 
ATGTCTAAGC TGACGCTAGC CGACATGAAC ATCTTGCTGT TTCGCTGCGA AGCAGAAGAA 
CAAGAGGACG GTGGAGGCTG TTACAGCATC CCAGGCTGGG AGGGCCTGAA GTATTCAGGA 
CTACAAGGTC TGATATCTGT GTTGGCGGAC ATCCGTCCCA CTAATGACCT TGGCCATCCT 
CTGTGTGCTA ACCTCAGAGA AGGAGACTGG CTCATAGACT TCGTCTCCAA CAGGCTGAAG 
CACAGAGATG GACCACTGGC ACAGGTGGGC CAGTGGTTGG GTGCCATGTT TGACTACCTG 
AGGCACTTCC CTCGATACCT CATCCCTTGT TACTTTGACG CCATCTTGGT GTCAACCTAC 
ACCACAGCTC TGGATGCCAC GTTCAAACTC ATGTCGAGCT TCATTCAGCG AGGTTCCACG 
TTTGTCCGTC ACCTGGCTCT CGGCTCAGTT CAGATGTGTG GAGTTGGACG TTTTCCACCT 
CTGCCTCCCA TTTCAACAAA AGTGCAGGAT GTCCCATACC GCATCAGTCC CATCACTGGG 
GAAAAGGAGC AGTGCTGTGT CTCACTGGCA GCAGGTCTTC CTCATTTTTC TGCTGGGATT 
TTCCGTTGTT GGGGTAGAGA CACTTTCATC GCTCTCAGAG GACTCATGCT TATAACAGGG 
AGACACACTG AGGCACGCAA CATCATCCTT GCTTTTGCTG GAACCCTGCG TCACGGTTTG 
ATCCCCAACT TGATGGGAGA GGGTCGCAAT GCCAGATACA ACTGCAGGGA TGCTGTGTGG 
TGGTGGCTGC AGTGCATTCA GGACTATACC AATCTCGTTC CTGAGGGACA TGAAATTCTG 
CGTTGCCCTG TCACCCGCAT GTACCCCACA GACGACTGTG AGCCCTGCAA ACCAGGGGAG 
GTGGAGCAGC CATTGTATGA TGTTATCCAG GAGGCACTGC AGCGCCATCT GGAGGGTATT 
TCCTTCAGAG AGAGGAATGC TGGACCCAAG ATGGACAGGA ACATGAGAGA TGAAGGGTTC 
AATGTGGTTG CTAAAGTGGA CCCAGCAACA GGTTTTGTTA CCGGAGGAAA CCGCTTCAAC 
TGTGGCACTT GGATGGATAA AATGGGGGAG AGTGACAGAG CGAGGAACAA GGGGATACCT 
GCAACTCCAA GAGACGGCGC GGCGGTGGAG ATAGTTGGTC TGAGTAAATC AGCAGTTCGT 
TGGCTTGTGG AGCTGCAGTC TAAAGAACTG TTCCCCTATG ATGGCGTTAA GGTGCACAGA 
GATGGAAAGG ATGTGTTCAT CTTGTATACT GAGTGGAACA AGCAGCTTCA GGAGTCCTTT 
GAAGTTGAAT TCTGGGTGTC TGGGGACTCA CAGGACCCCA ATGAGAAGCA CCCTGATTTG 
GTGCATAAAA AGGGAATTTA TAAAGATAGT TATGGAGCTT CCAATCCCTG GTGTGACTAC 
CAGCTGAGGC CCAACTTTAC CATCGCAATG GTGGTGGCTC CAGAGCTGTT CACGCTAGAA 
AGAGCATGGA AGTCTTTGGA AATAGCTGAA AAGAAACTCC TGGGACCACT GGGAATGAAA 
ACTCTTGACC CAGATGACAT GGTCTACTGT GGTGTTTACG ATAATGCACT GGACAACGAC 
AACTACAACC TGGCCAAGGG CTTTAACTAC CACCAAGGAC CTGAGTGGCT GTGGCCTGTT 
GGCTATTTTC TCCGTGCTAA GCTCTACTTT GCCAAGAGGT TAGGGGAGGA GACCTACTCC 
AAGACCGTCA TCCTAGTGAA AAATGTTCTG TCACGACATT GCACCCACTT GGAGAGGTCT 
CTATGGAAAG GCTTACCAGA GCTGACCAAT GAAAACGGCC AGCATTGCCC CTTCAGCTGT 
GAGACCCAGG CCTGGTCTCT GGCTACTGTG CTGGAGGTGC TCCATGATCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008431724.1
CDS	249..4862
Translation	MMLPQHLKQIRVLMLNEKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYADVERLGGGYVVVDPILRVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSYTWEDVGRLVEKLRTEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNESHLNALRRALWEDIFPQIKLWEFYQVNVENAVEKFRVLLQNGTKPDHSKAEGNKGIEIIQDLQCYRYGNRVNMDSALETFVPHSSSPQHIDECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCVVGNIVYERLAGHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWEHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDAAREVRPNLYVIAELFTGSELIDNVFVNRLGINSLIREAMSAGDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIQLRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPISTGEVGFQSGIIAGKLALNKLHQELAAQGFSQVYVDQVDTDIVAVTRHCPSTHQSVVTVCRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREAGSYKKDEKYINGMPEYTVEIKEHVPMVKSAVVKHAGVTSKDRSEFVQEIQFQKLTPGSIIAFRVCLDPKAQKLVGMLRFYLSQFSPKYRRGSVPDENPQEVLQKPLAQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWEGLKYSGLQGLISVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAMFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIQRGSTFVRHLALGSVQMCGVGRFPPLPPISTKVQDVPYRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLMGEGRNARYNCRDAVWWWLQCIQDYTNLVPEGHEILRCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKMDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGIPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVHRDGKDVFILYTEWNKQLQESFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKSLEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKRLGEETYSKTVILVKNVLSRHCTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_008433502.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008433502.2

ORF Insert Sequence:

ATGATGCTGC CTCAGCACCT AAAGCAAATC AGAGTACTCA TGCTTAACGA AAAGGAGAAT 
TTAGAGAGAA CTTTGTTCAG ACTTGAACAA GGTTTTGAGC TCCAGTTCCG CCTGGGTCCA 
AGCCTTCAAG GAAGGAGAGT TATAGTTCAC ACCAACTACC CACTTGATGG ACAGAAGTTC 
ATTCGAAACA ACTTCCGTGT TTTAGCCTGG AACTACCCAA CAGGAAGGGA GGATGACTCT 
GATAAGTACT GCTCCCTAGA GCTCAAGATT GCTGGATCCT ACCAGTACTA CTTTGGATAT 
GCAGATGTGG AGAGGTTAGG AGGAGGCTAC GTCGTCGTTG ATCCTATTCT TCGTGTTGGA 
GCAGACAACC ATGTCCTCCT GCTGGACTGT GTCACCATCC AGACATACTT GTCCAAATGT 
CTGGGACGCC TTGATGACTG GCCAGACAGA CTGCGAGTAG CCAAGGAGTC AGGTTACAAC 
ATGATCCACT TCACTCCTCT GCAGACTTTG GGAGAGTCTA GGTCCTGTTA CTCCTTAGCT 
GACCAGCTGA CCTTTAGCCT GGAATTCAGC CCAGATGGCA AGAGTTACAC CTGGGAAGAT 
GTGGGAAGGC TAGTGGAAAA ACTGAGGACG GAATGGAATA TGGTATCAAT CACTGATGTA 
GTGTATAATC ACACTGCTGC AAACAGTGTG TGGATAAAGG AACATCCAGA GTGTGGCTAC 
AACCTGATGA ACTCTCCTCA CCTGCGTCCT GCCTGGGTCT TAGACAGAGC TATCTGGCAT 
TTAACCACTA GGGTAGCAGA TGGACGGTAC AAAGAGAAGG GACTTCCTTC TGACATTACC 
AACGAGAGCC ACCTAAATGC TCTCCGCAGG GCGCTTTGGG AGGACATTTT TCCTCAAATC 
AAACTTTGGG AGTTCTACCA GGTCAACGTG GAAAATGCTG TGGAGAAGTT CAGAGTACTG 
CTGCAAAACG GCACAAAGCC AGATCATAGC AAGGCTGAAG GAAATAAAGG CATAGAGATC 
ATTCAAGACC TCCAGTGCTA TCGCTATGGC AACAGAGTGA ACATGGACTC AGCTTTGGAG 
ACATTTGTTC CTCACAGCTC CTCTCCACAG CACATTGATG AGTGCTGTGG TTGGCTGAAA 
CAGAAGTTGA CTGAGATGAA TGAAGAACAG TACCACATCA TACACCAGCA TCAGGAACAG 
GCCGTCAACT GCGTTGTGGG GAACATAGTG TATGAACGTC TAGCTGGCCA CGGTCCTAAA 
CTGGGTCCTG TTACCAGGAA AGAGCCTCTT GTCACCAGGT ATTTCACCTT CCCCTTTGAG 
GAAATGACTT TAGAGCAGGA GATGGAGTTT TTAAACCAAC CAGATAAGAT GTGCCACTTC 
TTGGCTCACA ACGGCTGGGT GATGGGTGAT GATCCATTGC GCAACTTTGC TGAACCAGGA 
TCCAATGTGT ACCTGCGTCG GGAGCTGATC TGCTGGGGGG ACAGTGTCAA ACTCCGCTAT 
GGAAAAGGAC CTGAGGATTG CCCCTACCTG TGGGAGCACA TGCAGAAATA CACTGAAATT 
ACTGCCAAAT ATTTCCACGG AGTCCGATTG GACAACTGCC ACTCTACTCC CTTGCATGTT 
GCTGAGTTCA TGTTAGATGC AGCCAGAGAG GTCAGACCCA ACCTGTATGT AATAGCTGAG 
CTCTTTACAG GCAGTGAACT CATAGACAAT GTGTTTGTTA ACCGACTGGG AATCAACAGC 
CTCATAAGAG AGGCCATGTC CGCCGGAGAC AGCCATGAAG AGGGCCGGCT GGTCTATCGT 
TACGGTGGAG AGCCAGTCGG AGCCTTCGTT CAGCCCAGCC TGCGTCCTCT CATGCCTTCT 
ATCGCTCACG CCATGTTCTT GGATGTGACC CATGACAATG AGTGTCCCAT TCAGCTGCGG 
TCAGCTCTCG ACTCGTTGCC CAGTTCTGCT ATCGTTTCCA TGGCCTGCTG TGCTACTGGC 
TCCACCAGAG GATATGATGA ACTGGTCCCA CATCAGATAT CTGTGGTGAA GGAGGAGCGT 
TTTTATCCCA AGTGGAATCC CTCTTCAGCA CCCATCTCTA CTGGTGAAGT TGGCTTTCAG 
AGCGGCATCA TCGCTGGGAA ACTGGCTCTC AACAAGCTAC ACCAGGAGCT GGCTGCCCAG 
GGCTTTTCAC AGGTGTATGT GGACCAGGTT GATACAGATA TTGTTGCAGT TACTCGTCAC 
TGCCCCAGCA CACACCAGTC TGTGGTAACA GTGTGTAGGA CAGCCTTCAA GAACCCCAAA 
ACCCATCAGT ACAGCAACAA TGTCCCACCT ATGTTCATAC CTGGCAAGAT TGAAGAAATT 
GTACTGGAGG CAAGGACAGT GGAGAGAGAG GCTGGGAGTT ACAAGAAGGA TGAGAAATAC 
ATAAATGGCA TGCCAGAATA CACAGTGGAA ATAAAAGAGC ATGTACCGAT GGTGAAAAGT 
GCAGTGGTGA AACATGCTGG AGTGACCTCT AAAGACCGCT CAGAGTTTGT GCAAGAAATT 
CAATTCCAGA AATTGACTCC TGGTAGCATC ATTGCCTTCA GAGTCTGTTT GGATCCCAAA 
GCACAGAAGC TGGTGGGCAT GTTGAGGTTT TATCTGTCCC AGTTCAGTCC AAAGTACAGG 
AGGGGCAGTG TACCTGATGA GAATCCACAA GAGGTGCTGC AGAAACCCCT TGCACAGTTA 
ATGTCTAAGC TGACGCTAGC CGACATGAAC ATCTTGCTGT TTCGCTGCGA AGCAGAAGAA 
CAAGAGGACG GTGGAGGCTG TTACAGCATC CCAGGCTGGG AGGGCCTGAA GTATTCAGGA 
CTACAAGGTC TGATATCTGT GTTGGCGGAC ATCCGTCCCA CTAATGACCT TGGCCATCCT 
CTGTGTGCTA ACCTCAGAGA AGGAGACTGG CTCATAGACT TCGTCTCCAA CAGGCTGAAG 
CACAGAGATG GACCACTGGC ACAGGTGGGC CAGTGGTTGG GTGCCATGTT TGACTACCTG 
AGGCACTTCC CTCGATACCT CATCCCTTGT TACTTTGACG CCATCTTGGT GTCAACCTAC 
ACCACAGCTC TGGATGCCAC GTTCAAACTC ATGTCGAGCT TCATTCAGCG AGGTTCCACG 
TTTGTCCGTC ACCTGGCTCT CGGCTCAGTT CAGATGTGTG GAGTTGGACG TTTTCCACCT 
CTGCCTCCCA TTTCAACAAA AGTGCAGGAT GTCCCATACC GCATCAGTCC CATCACTGGG 
GAAAAGGAGC AGTGCTGTGT CTCACTGGCA GCAGGTCTTC CTCATTTTTC TGCTGGGATT 
TTCCGTTGTT GGGGTAGAGA CACTTTCATC GCTCTCAGAG GACTCATGCT TATAACAGGG 
AGACACACTG AGGCACGCAA CATCATCCTT GCTTTTGCTG GAACCCTGCG TCACGGTTTG 
ATCCCCAACT TGATGGGAGA GGGTCGCAAT GCCAGATACA ACTGCAGGGA TGCTGTGTGG 
TGGTGGCTGC AGTGCATTCA GGACTATACC AATCTCGTTC CTGAGGGACA TGAAATTCTG 
CGTTGCCCTG TCACCCGCAT GTACCCCACA GACGACTGTG AGCCCTGCAA ACCAGGGGAG 
GTGGAGCAGC CATTGTATGA TGTTATCCAG GAGGCACTGC AGCGCCATCT GGAGGGTATT 
TCCTTCAGAG AGAGGAATGC TGGACCCAAG ATGGACAGGA ACATGAGAGA TGAAGGGTTC 
AATGTGGTTG CTAAAGTGGA CCCAGCAACA GGTTTTGTTA CCGGAGGAAA CCGCTTCAAC 
TGTGGCACTT GGATGGATAA AATGGGGGAG AGTGACAGAG CGAGGAACAA GGGGATACCT 
GCAACTCCAA GAGACGGCGC GGCGGTGGAG ATAGTTGGTC TGAGTAAATC AGCAGTTCGT 
TGGCTTGTGG AGCTGCAGTC TAAAGAACTG TTCCCCTATG ATGGCGTTAA GGTGCACAGA 
GATGGAAAGG ATGTGTTCAT CTTGTATACT GAGTGGAACA AGCAGCTTCA GGAGTCCTTT 
GAAGTTGAAT TCTGGGTGTC TGGGGACTCA CAGGACCCCA ATGAGAAGCA CCCTGATTTG 
GTGCATAAAA AGGGAATTTA TAAAGATAGT TATGGAGCTT CCAATCCCTG GTGTGACTAC 
CAGCTGAGGC CCAACTTTAC CATCGCAATG GTGGTGGCTC CAGAGCTGTT CACGCTAGAA 
AGAGCATGGA AGTCTTTGGA AATAGCTGAA AAGAAACTCC TGGGACCACT GGGAATGAAA 
ACTCTTGACC CAGATGACAT GGTCTACTGT GGTGTTTACG ATAATGCACT GGACAACGAC 
AACTACAACC TGGCCAAGGG CTTTAACTAC CACCAAGGAC CTGAGTGGCT GTGGCCTGTT 
GGCTATTTTC TCCGTGCTAA GCTCTACTTT GCCAAGAGGT TAGGGGAGGA GACCTACTCC 
AAGACCGTCA TCCTAGTGAA AAATGTTCTG TCACGACATT GCACCCACTT GGAGAGGTCT 
CTATGGAAAG GCTTACCAGA GCTGACCAAT GAAAACGGCC AGCATTGCCC CTTCAGCTGT 
GAGACCCAGG CCTGGTCTCT GGCTACTGTG CTGGAGGTGC TCCATGATCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_008433503.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008431725.1 Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPo179980
Clone ID Related Accession (Same CDS sequence)	XM_008433503.2
Accession Version	XM_008433503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	glycogen debranching enzyme isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008433503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTAC TTACAGGTTT CTTCTTCAGT TTGAGACTCC TCATTGTAAT CTGTCTAATG 
CCCGTCTCTT TGGAGACCAG GGTTCCCTCC AAGTTCAGGA GAACTTCCAT GATGCTGCCT 
CAGCACCTAA AGCAAATCAG AGTACTCATG CTTAACGAAA AGGAGAATTT AGAGAGAACT 
TTGTTCAGAC TTGAACAAGG TTTTGAGCTC CAGTTCCGCC TGGGTCCAAG CCTTCAAGGA 
AGGAGAGTTA TAGTTCACAC CAACTACCCA CTTGATGGAC AGAAGTTCAT TCGAAACAAC 
TTCCGTGTTT TAGCCTGGAA CTACCCAACA GGAAGGGAGG ATGACTCTGA TAAGTACTGC 
TCCCTAGAGC TCAAGATTGC TGGATCCTAC CAGTACTACT TTGGATATGC AGATGTGGAG 
AGGTTAGGAG GAGGCTACGT CGTCGTTGAT CCTATTCTTC GTGTTGGAGC AGACAACCAT 
GTCCTCCTGC TGGACTGTGT CACCATCCAG ACATACTTGT CCAAATGTCT GGGACGCCTT 
GATGACTGGC CAGACAGACT GCGAGTAGCC AAGGAGTCAG GTTACAACAT GATCCACTTC 
ACTCCTCTGC AGACTTTGGG AGAGTCTAGG TCCTGTTACT CCTTAGCTGA CCAGCTGACC 
TTTAGCCTGG AATTCAGCCC AGATGGCAAG AGTTACACCT GGGAAGATGT GGGAAGGCTA 
GTGGAAAAAC TGAGGACGGA ATGGAATATG GTATCAATCA CTGATGTAGT GTATAATCAC 
ACTGCTGCAA ACAGTGTGTG GATAAAGGAA CATCCAGAGT GTGGCTACAA CCTGATGAAC 
TCTCCTCACC TGCGTCCTGC CTGGGTCTTA GACAGAGCTA TCTGGCATTT AACCACTAGG 
GTAGCAGATG GACGGTACAA AGAGAAGGGA CTTCCTTCTG ACATTACCAA CGAGAGCCAC 
CTAAATGCTC TCCGCAGGGC GCTTTGGGAG GACATTTTTC CTCAAATCAA ACTTTGGGAG 
TTCTACCAGG TCAACGTGGA AAATGCTGTG GAGAAGTTCA GAGTACTGCT GCAAAACGGC 
ACAAAGCCAG ATCATAGCAA GGCTGAAGGA AATAAAGGCA TAGAGATCAT TCAAGACCTC 
CAGTGCTATC GCTATGGCAA CAGAGTGAAC ATGGACTCAG CTTTGGAGAC ATTTGTTCCT 
CACAGCTCCT CTCCACAGCA CATTGATGAG TGCTGTGGTT GGCTGAAACA GAAGTTGACT 
GAGATGAATG AAGAACAGTA CCACATCATA CACCAGCATC AGGAACAGGC CGTCAACTGC 
GTTGTGGGGA ACATAGTGTA TGAACGTCTA GCTGGCCACG GTCCTAAACT GGGTCCTGTT 
ACCAGGAAAG AGCCTCTTGT CACCAGGTAT TTCACCTTCC CCTTTGAGGA AATGACTTTA 
GAGCAGGAGA TGGAGTTTTT AAACCAACCA GATAAGATGT GCCACTTCTT GGCTCACAAC 
GGCTGGGTGA TGGGTGATGA TCCATTGCGC AACTTTGCTG AACCAGGATC CAATGTGTAC 
CTGCGTCGGG AGCTGATCTG CTGGGGGGAC AGTGTCAAAC TCCGCTATGG AAAAGGACCT 
GAGGATTGCC CCTACCTGTG GGAGCACATG CAGAAATACA CTGAAATTAC TGCCAAATAT 
TTCCACGGAG TCCGATTGGA CAACTGCCAC TCTACTCCCT TGCATGTTGC TGAGTTCATG 
TTAGATGCAG CCAGAGAGGT CAGACCCAAC CTGTATGTAA TAGCTGAGCT CTTTACAGGC 
AGTGAACTCA TAGACAATGT GTTTGTTAAC CGACTGGGAA TCAACAGCCT CATAAGAGAG 
GCCATGTCCG CCGGAGACAG CCATGAAGAG GGCCGGCTGG TCTATCGTTA CGGTGGAGAG 
CCAGTCGGAG CCTTCGTTCA GCCCAGCCTG CGTCCTCTCA TGCCTTCTAT CGCTCACGCC 
ATGTTCTTGG ATGTGACCCA TGACAATGAG TGTCCCATTC AGCTGCGGTC AGCTCTCGAC 
TCGTTGCCCA GTTCTGCTAT CGTTTCCATG GCCTGCTGTG CTACTGGCTC CACCAGAGGA 
TATGATGAAC TGGTCCCACA TCAGATATCT GTGGTGAAGG AGGAGCGTTT TTATCCCAAG 
TGGAATCCCT CTTCAGCACC CATCTCTACT GGTGAAGTTG GCTTTCAGAG CGGCATCATC 
GCTGGGAAAC TGGCTCTCAA CAAGCTACAC CAGGAGCTGG CTGCCCAGGG CTTTTCACAG 
GTGTATGTGG ACCAGGTTGA TACAGATATT GTTGCAGTTA CTCGTCACTG CCCCAGCACA 
CACCAGTCTG TGGTAACAGT GTGTAGGACA GCCTTCAAGA ACCCCAAAAC CCATCAGTAC 
AGCAACAATG TCCCACCTAT GTTCATACCT GGCAAGATTG AAGAAATTGT ACTGGAGGCA 
AGGACAGTGG AGAGAGAGGC TGGGAGTTAC AAGAAGGATG AGAAATACAT AAATGGCATG 
CCAGAATACA CAGTGGAAAT AAAAGAGCAT GTACCGATGG TGAAAAGTGC AGTGGTGAAA 
CATGCTGGAG TGACCTCTAA AGACCGCTCA GAGTTTGTGC AAGAAATTCA ATTCCAGAAA 
TTGACTCCTG GTAGCATCAT TGCCTTCAGA GTCTGTTTGG ATCCCAAAGC ACAGAAGCTG 
GTGGGCATGT TGAGGTTTTA TCTGTCCCAG TTCAGTCCAA AGTACAGGAG GGGCAGTGTA 
CCTGATGAGA ATCCACAAGA GGTGCTGCAG AAACCCCTTG CACAGTTAAT GTCTAAGCTG 
ACGCTAGCCG ACATGAACAT CTTGCTGTTT CGCTGCGAAG CAGAAGAACA AGAGGACGGT 
GGAGGCTGTT ACAGCATCCC AGGCTGGGAG GGCCTGAAGT ATTCAGGACT ACAAGGTCTG 
ATATCTGTGT TGGCGGACAT CCGTCCCACT AATGACCTTG GCCATCCTCT GTGTGCTAAC 
CTCAGAGAAG GAGACTGGCT CATAGACTTC GTCTCCAACA GGCTGAAGCA CAGAGATGGA 
CCACTGGCAC AGGTGGGCCA GTGGTTGGGT GCCATGTTTG ACTACCTGAG GCACTTCCCT 
CGATACCTCA TCCCTTGTTA CTTTGACGCC ATCTTGGTGT CAACCTACAC CACAGCTCTG 
GATGCCACGT TCAAACTCAT GTCGAGCTTC ATTCAGCGAG GTTCCACGTT TGTCCGTCAC 
CTGGCTCTCG GCTCAGTTCA GATGTGTGGA GTTGGACGTT TTCCACCTCT GCCTCCCATT 
TCAACAAAAG TGCAGGATGT CCCATACCGC ATCAGTCCCA TCACTGGGGA AAAGGAGCAG 
TGCTGTGTCT CACTGGCAGC AGGTCTTCCT CATTTTTCTG CTGGGATTTT CCGTTGTTGG 
GGTAGAGACA CTTTCATCGC TCTCAGAGGA CTCATGCTTA TAACAGGGAG ACACACTGAG 
GCACGCAACA TCATCCTTGC TTTTGCTGGA ACCCTGCGTC ACGGTTTGAT CCCCAACTTG 
ATGGGAGAGG GTCGCAATGC CAGATACAAC TGCAGGGATG CTGTGTGGTG GTGGCTGCAG 
TGCATTCAGG ACTATACCAA TCTCGTTCCT GAGGGACATG AAATTCTGCG TTGCCCTGTC 
ACCCGCATGT ACCCCACAGA CGACTGTGAG CCCTGCAAAC CAGGGGAGGT GGAGCAGCCA 
TTGTATGATG TTATCCAGGA GGCACTGCAG CGCCATCTGG AGGGTATTTC CTTCAGAGAG 
AGGAATGCTG GACCCAAGAT GGACAGGAAC ATGAGAGATG AAGGGTTCAA TGTGGTTGCT 
AAAGTGGACC CAGCAACAGG TTTTGTTACC GGAGGAAACC GCTTCAACTG TGGCACTTGG 
ATGGATAAAA TGGGGGAGAG TGACAGAGCG AGGAACAAGG GGATACCTGC AACTCCAAGA 
GACGGCGCGG CGGTGGAGAT AGTTGGTCTG AGTAAATCAG CAGTTCGTTG GCTTGTGGAG 
CTGCAGTCTA AAGAACTGTT CCCCTATGAT GGCGTTAAGG TGCACAGAGA TGGAAAGGAT 
GTGTTCATCT TGTATACTGA GTGGAACAAG CAGCTTCAGG AGTCCTTTGA AGTTGAATTC 
TGGGTGTCTG GGGACTCACA GGACCCCAAT GAGAAGCACC CTGATTTGGT GCATAAAAAG 
GGAATTTATA AAGATAGTTA TGGAGCTTCC AATCCCTGGT GTGACTACCA GCTGAGGCCC 
AACTTTACCA TCGCAATGGT GGTGGCTCCA GAGCTGTTCA CGCTAGAAAG AGCATGGAAG 
TCTTTGGAAA TAGCTGAAAA GAAACTCCTG GGACCACTGG GAATGAAAAC TCTTGACCCA 
GATGACATGG TCTACTGTGG TGTTTACGAT AATGCACTGG ACAACGACAA CTACAACCTG 
GCCAAGGGCT TTAACTACCA CCAAGGACCT GTAAGAGTGG CTGTGGCCTG TTGGCTATTT 
TCTCCGTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008431725.1
CDS	199..4710
Translation	MLLLTGFFFSLRLLIVICLMPVSLETRVPSKFRRTSMMLPQHLKQIRVLMLNEKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYADVERLGGGYVVVDPILRVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSYTWEDVGRLVEKLRTEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNESHLNALRRALWEDIFPQIKLWEFYQVNVENAVEKFRVLLQNGTKPDHSKAEGNKGIEIIQDLQCYRYGNRVNMDSALETFVPHSSSPQHIDECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCVVGNIVYERLAGHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWEHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDAAREVRPNLYVIAELFTGSELIDNVFVNRLGINSLIREAMSAGDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIQLRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPISTGEVGFQSGIIAGKLALNKLHQELAAQGFSQVYVDQVDTDIVAVTRHCPSTHQSVVTVCRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREAGSYKKDEKYINGMPEYTVEIKEHVPMVKSAVVKHAGVTSKDRSEFVQEIQFQKLTPGSIIAFRVCLDPKAQKLVGMLRFYLSQFSPKYRRGSVPDENPQEVLQKPLAQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWEGLKYSGLQGLISVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAMFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIQRGSTFVRHLALGSVQMCGVGRFPPLPPISTKVQDVPYRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLMGEGRNARYNCRDAVWWWLQCIQDYTNLVPEGHEILRCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKMDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGIPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVHRDGKDVFILYTEWNKQLQESFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKSLEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPVRVAVACWLFSPC

Target ORF information:

RefSeq Version	XM_008433503.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008433503.2

ORF Insert Sequence:

ATGCTGCTAC TTACAGGTTT CTTCTTCAGT TTGAGACTCC TCATTGTAAT CTGTCTAATG 
CCCGTCTCTT TGGAGACCAG GGTTCCCTCC AAGTTCAGGA GAACTTCCAT GATGCTGCCT 
CAGCACCTAA AGCAAATCAG AGTACTCATG CTTAACGAAA AGGAGAATTT AGAGAGAACT 
TTGTTCAGAC TTGAACAAGG TTTTGAGCTC CAGTTCCGCC TGGGTCCAAG CCTTCAAGGA 
AGGAGAGTTA TAGTTCACAC CAACTACCCA CTTGATGGAC AGAAGTTCAT TCGAAACAAC 
TTCCGTGTTT TAGCCTGGAA CTACCCAACA GGAAGGGAGG ATGACTCTGA TAAGTACTGC 
TCCCTAGAGC TCAAGATTGC TGGATCCTAC CAGTACTACT TTGGATATGC AGATGTGGAG 
AGGTTAGGAG GAGGCTACGT CGTCGTTGAT CCTATTCTTC GTGTTGGAGC AGACAACCAT 
GTCCTCCTGC TGGACTGTGT CACCATCCAG ACATACTTGT CCAAATGTCT GGGACGCCTT 
GATGACTGGC CAGACAGACT GCGAGTAGCC AAGGAGTCAG GTTACAACAT GATCCACTTC 
ACTCCTCTGC AGACTTTGGG AGAGTCTAGG TCCTGTTACT CCTTAGCTGA CCAGCTGACC 
TTTAGCCTGG AATTCAGCCC AGATGGCAAG AGTTACACCT GGGAAGATGT GGGAAGGCTA 
GTGGAAAAAC TGAGGACGGA ATGGAATATG GTATCAATCA CTGATGTAGT GTATAATCAC 
ACTGCTGCAA ACAGTGTGTG GATAAAGGAA CATCCAGAGT GTGGCTACAA CCTGATGAAC 
TCTCCTCACC TGCGTCCTGC CTGGGTCTTA GACAGAGCTA TCTGGCATTT AACCACTAGG 
GTAGCAGATG GACGGTACAA AGAGAAGGGA CTTCCTTCTG ACATTACCAA CGAGAGCCAC 
CTAAATGCTC TCCGCAGGGC GCTTTGGGAG GACATTTTTC CTCAAATCAA ACTTTGGGAG 
TTCTACCAGG TCAACGTGGA AAATGCTGTG GAGAAGTTCA GAGTACTGCT GCAAAACGGC 
ACAAAGCCAG ATCATAGCAA GGCTGAAGGA AATAAAGGCA TAGAGATCAT TCAAGACCTC 
CAGTGCTATC GCTATGGCAA CAGAGTGAAC ATGGACTCAG CTTTGGAGAC ATTTGTTCCT 
CACAGCTCCT CTCCACAGCA CATTGATGAG TGCTGTGGTT GGCTGAAACA GAAGTTGACT 
GAGATGAATG AAGAACAGTA CCACATCATA CACCAGCATC AGGAACAGGC CGTCAACTGC 
GTTGTGGGGA ACATAGTGTA TGAACGTCTA GCTGGCCACG GTCCTAAACT GGGTCCTGTT 
ACCAGGAAAG AGCCTCTTGT CACCAGGTAT TTCACCTTCC CCTTTGAGGA AATGACTTTA 
GAGCAGGAGA TGGAGTTTTT AAACCAACCA GATAAGATGT GCCACTTCTT GGCTCACAAC 
GGCTGGGTGA TGGGTGATGA TCCATTGCGC AACTTTGCTG AACCAGGATC CAATGTGTAC 
CTGCGTCGGG AGCTGATCTG CTGGGGGGAC AGTGTCAAAC TCCGCTATGG AAAAGGACCT 
GAGGATTGCC CCTACCTGTG GGAGCACATG CAGAAATACA CTGAAATTAC TGCCAAATAT 
TTCCACGGAG TCCGATTGGA CAACTGCCAC TCTACTCCCT TGCATGTTGC TGAGTTCATG 
TTAGATGCAG CCAGAGAGGT CAGACCCAAC CTGTATGTAA TAGCTGAGCT CTTTACAGGC 
AGTGAACTCA TAGACAATGT GTTTGTTAAC CGACTGGGAA TCAACAGCCT CATAAGAGAG 
GCCATGTCCG CCGGAGACAG CCATGAAGAG GGCCGGCTGG TCTATCGTTA CGGTGGAGAG 
CCAGTCGGAG CCTTCGTTCA GCCCAGCCTG CGTCCTCTCA TGCCTTCTAT CGCTCACGCC 
ATGTTCTTGG ATGTGACCCA TGACAATGAG TGTCCCATTC AGCTGCGGTC AGCTCTCGAC 
TCGTTGCCCA GTTCTGCTAT CGTTTCCATG GCCTGCTGTG CTACTGGCTC CACCAGAGGA 
TATGATGAAC TGGTCCCACA TCAGATATCT GTGGTGAAGG AGGAGCGTTT TTATCCCAAG 
TGGAATCCCT CTTCAGCACC CATCTCTACT GGTGAAGTTG GCTTTCAGAG CGGCATCATC 
GCTGGGAAAC TGGCTCTCAA CAAGCTACAC CAGGAGCTGG CTGCCCAGGG CTTTTCACAG 
GTGTATGTGG ACCAGGTTGA TACAGATATT GTTGCAGTTA CTCGTCACTG CCCCAGCACA 
CACCAGTCTG TGGTAACAGT GTGTAGGACA GCCTTCAAGA ACCCCAAAAC CCATCAGTAC 
AGCAACAATG TCCCACCTAT GTTCATACCT GGCAAGATTG AAGAAATTGT ACTGGAGGCA 
AGGACAGTGG AGAGAGAGGC TGGGAGTTAC AAGAAGGATG AGAAATACAT AAATGGCATG 
CCAGAATACA CAGTGGAAAT AAAAGAGCAT GTACCGATGG TGAAAAGTGC AGTGGTGAAA 
CATGCTGGAG TGACCTCTAA AGACCGCTCA GAGTTTGTGC AAGAAATTCA ATTCCAGAAA 
TTGACTCCTG GTAGCATCAT TGCCTTCAGA GTCTGTTTGG ATCCCAAAGC ACAGAAGCTG 
GTGGGCATGT TGAGGTTTTA TCTGTCCCAG TTCAGTCCAA AGTACAGGAG GGGCAGTGTA 
CCTGATGAGA ATCCACAAGA GGTGCTGCAG AAACCCCTTG CACAGTTAAT GTCTAAGCTG 
ACGCTAGCCG ACATGAACAT CTTGCTGTTT CGCTGCGAAG CAGAAGAACA AGAGGACGGT 
GGAGGCTGTT ACAGCATCCC AGGCTGGGAG GGCCTGAAGT ATTCAGGACT ACAAGGTCTG 
ATATCTGTGT TGGCGGACAT CCGTCCCACT AATGACCTTG GCCATCCTCT GTGTGCTAAC 
CTCAGAGAAG GAGACTGGCT CATAGACTTC GTCTCCAACA GGCTGAAGCA CAGAGATGGA 
CCACTGGCAC AGGTGGGCCA GTGGTTGGGT GCCATGTTTG ACTACCTGAG GCACTTCCCT 
CGATACCTCA TCCCTTGTTA CTTTGACGCC ATCTTGGTGT CAACCTACAC CACAGCTCTG 
GATGCCACGT TCAAACTCAT GTCGAGCTTC ATTCAGCGAG GTTCCACGTT TGTCCGTCAC 
CTGGCTCTCG GCTCAGTTCA GATGTGTGGA GTTGGACGTT TTCCACCTCT GCCTCCCATT 
TCAACAAAAG TGCAGGATGT CCCATACCGC ATCAGTCCCA TCACTGGGGA AAAGGAGCAG 
TGCTGTGTCT CACTGGCAGC AGGTCTTCCT CATTTTTCTG CTGGGATTTT CCGTTGTTGG 
GGTAGAGACA CTTTCATCGC TCTCAGAGGA CTCATGCTTA TAACAGGGAG ACACACTGAG 
GCACGCAACA TCATCCTTGC TTTTGCTGGA ACCCTGCGTC ACGGTTTGAT CCCCAACTTG 
ATGGGAGAGG GTCGCAATGC CAGATACAAC TGCAGGGATG CTGTGTGGTG GTGGCTGCAG 
TGCATTCAGG ACTATACCAA TCTCGTTCCT GAGGGACATG AAATTCTGCG TTGCCCTGTC 
ACCCGCATGT ACCCCACAGA CGACTGTGAG CCCTGCAAAC CAGGGGAGGT GGAGCAGCCA 
TTGTATGATG TTATCCAGGA GGCACTGCAG CGCCATCTGG AGGGTATTTC CTTCAGAGAG 
AGGAATGCTG GACCCAAGAT GGACAGGAAC ATGAGAGATG AAGGGTTCAA TGTGGTTGCT 
AAAGTGGACC CAGCAACAGG TTTTGTTACC GGAGGAAACC GCTTCAACTG TGGCACTTGG 
ATGGATAAAA TGGGGGAGAG TGACAGAGCG AGGAACAAGG GGATACCTGC AACTCCAAGA 
GACGGCGCGG CGGTGGAGAT AGTTGGTCTG AGTAAATCAG CAGTTCGTTG GCTTGTGGAG 
CTGCAGTCTA AAGAACTGTT CCCCTATGAT GGCGTTAAGG TGCACAGAGA TGGAAAGGAT 
GTGTTCATCT TGTATACTGA GTGGAACAAG CAGCTTCAGG AGTCCTTTGA AGTTGAATTC 
TGGGTGTCTG GGGACTCACA GGACCCCAAT GAGAAGCACC CTGATTTGGT GCATAAAAAG 
GGAATTTATA AAGATAGTTA TGGAGCTTCC AATCCCTGGT GTGACTACCA GCTGAGGCCC 
AACTTTACCA TCGCAATGGT GGTGGCTCCA GAGCTGTTCA CGCTAGAAAG AGCATGGAAG 
TCTTTGGAAA TAGCTGAAAA GAAACTCCTG GGACCACTGG GAATGAAAAC TCTTGACCCA 
GATGACATGG TCTACTGTGG TGTTTACGAT AATGCACTGG ACAACGACAA CTACAACCTG 
GCCAAGGGCT TTAACTACCA CCAAGGACCT GTAAGAGTGG CTGTGGCCTG TTGGCTATTT 
TCTCCGTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_017309976.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017165465.1 Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPo179981
Clone ID Related Accession (Same CDS sequence)	XM_017309976.1
Accession Version	XM_017309976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	glycogen debranching enzyme isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTCT CTTTGGAGAC CAGGGTTCCC TCCAAGTTCA GGAGAACTTC CATGATGCTG 
CCTCAGCACC TAAAGCAAAT CAGAGTACTC ATGCTTAACG AAAAGGAGAA TTTAGAGAGA 
ACTTTGTTCA GACTTGAACA AGGTTTTGAG CTCCAGTTCC GCCTGGGTCC AAGCCTTCAA 
GGAAGGAGAG TTATAGTTCA CACCAACTAC CCACTTGATG GACAGAAGTT CATTCGAAAC 
AACTTCCGTG TTTTAGCCTG GAACTACCCA ACAGGAAGGG AGGATGACTC TGATAAGTAC 
TGCTCCCTAG AGCTCAAGAT TGCTGGATCC TACCAGTACT ACTTTGGATA TGCAGATGTG 
GAGAGGTTAG GAGGAGGCTA CGTCGTCGTT GATCCTATTC TTCGTGTTGG AGCAGACAAC 
CATGTCCTCC TGCTGGACTG TGTCACCATC CAGACATACT TGTCCAAATG TCTGGGACGC 
CTTGATGACT GGCCAGACAG ACTGCGAGTA GCCAAGGAGT CAGGTTACAA CATGATCCAC 
TTCACTCCTC TGCAGACTTT GGGAGAGTCT AGGTCCTGTT ACTCCTTAGC TGACCAGCTG 
ACCTTTAGCC TGGAATTCAG CCCAGATGGC AAGAGTTACA CCTGGGAAGA TGTGGGAAGG 
CTAGTGGAAA AACTGAGGAC GGAATGGAAT ATGGTATCAA TCACTGATGT AGTGTATAAT 
CACACTGCTG CAAACAGTGT GTGGATAAAG GAACATCCAG AGTGTGGCTA CAACCTGATG 
AACTCTCCTC ACCTGCGTCC TGCCTGGGTC TTAGACAGAG CTATCTGGCA TTTAACCACT 
AGGGTAGCAG ATGGACGGTA CAAAGAGAAG GGACTTCCTT CTGACATTAC CAACGAGAGC 
CACCTAAATG CTCTCCGCAG GGCGCTTTGG GAGGACATTT TTCCTCAAAT CAAACTTTGG 
GAGTTCTACC AGGTCAACGT GGAAAATGCT GTGGAGAAGT TCAGAGTACT GCTGCAAAAC 
GGCACAAAGC CAGATCATAG CAAGGCTGAA GGAAATAAAG GCATAGAGAT CATTCAAGAC 
CTCCAGTGCT ATCGCTATGG CAACAGAGTG AACATGGACT CAGCTTTGGA GACATTTGTT 
CCTCACAGCT CCTCTCCACA GCACATTGAT GAGTGCTGTG GTTGGCTGAA ACAGAAGTTG 
ACTGAGATGA ATGAAGAACA GTACCACATC ATACACCAGC ATCAGGAACA GGCCGTCAAC 
TGCGTTGTGG GGAACATAGT GTATGAACGT CTAGCTGGCC ACGGTCCTAA ACTGGGTCCT 
GTTACCAGGA AAGAGCCTCT TGTCACCAGG TATTTCACCT TCCCCTTTGA GGAAATGACT 
TTAGAGCAGG AGATGGAGTT TTTAAACCAA CCAGATAAGA TGTGCCACTT CTTGGCTCAC 
AACGGCTGGG TGATGGGTGA TGATCCATTG CGCAACTTTG CTGAACCAGG ATCCAATGTG 
TACCTGCGTC GGGAGCTGAT CTGCTGGGGG GACAGTGTCA AACTCCGCTA TGGAAAAGGA 
CCTGAGGATT GCCCCTACCT GTGGGAGCAC ATGCAGAAAT ACACTGAAAT TACTGCCAAA 
TATTTCCACG GAGTCCGATT GGACAACTGC CACTCTACTC CCTTGCATGT TGCTGAGTTC 
ATGTTAGATG CAGCCAGAGA GGTCAGACCC AACCTGTATG TAATAGCTGA GCTCTTTACA 
GGCAGTGAAC TCATAGACAA TGTGTTTGTT AACCGACTGG GAATCAACAG CCTCATAAGA 
GAGGCCATGT CCGCCGGAGA CAGCCATGAA GAGGGCCGGC TGGTCTATCG TTACGGTGGA 
GAGCCAGTCG GAGCCTTCGT TCAGCCCAGC CTGCGTCCTC TCATGCCTTC TATCGCTCAC 
GCCATGTTCT TGGATGTGAC CCATGACAAT GAGTGTCCCA TTCAGCTGCG GTCAGCTCTC 
GACTCGTTGC CCAGTTCTGC TATCGTTTCC ATGGCCTGCT GTGCTACTGG CTCCACCAGA 
GGATATGATG AACTGGTCCC ACATCAGATA TCTGTGGTGA AGGAGGAGCG TTTTTATCCC 
AAGTGGAATC CCTCTTCAGC ACCCATCTCT ACTGGTGAAG TTGGCTTTCA GAGCGGCATC 
ATCGCTGGGA AACTGGCTCT CAACAAGCTA CACCAGGAGC TGGCTGCCCA GGGCTTTTCA 
CAGGTGTATG TGGACCAGGT TGATACAGAT ATTGTTGCAG TTACTCGTCA CTGCCCCAGC 
ACACACCAGT CTGTGGTAAC AGTGTGTAGG ACAGCCTTCA AGAACCCCAA AACCCATCAG 
TACAGCAACA ATGTCCCACC TATGTTCATA CCTGGCAAGA TTGAAGAAAT TGTACTGGAG 
GCAAGGACAG TGGAGAGAGA GGCTGGGAGT TACAAGAAGG ATGAGAAATA CATAAATGGC 
ATGCCAGAAT ACACAGTGGA AATAAAAGAG CATGTACCGA TGGTGAAAAG TGCAGTGGTG 
AAACATGCTG GAGTGACCTC TAAAGACCGC TCAGAGTTTG TGCAAGAAAT TCAATTCCAG 
AAATTGACTC CTGGTAGCAT CATTGCCTTC AGAGTCTGTT TGGATCCCAA AGCACAGAAG 
CTGGTGGGCA TGTTGAGGTT TTATCTGTCC CAGTTCAGTC CAAAGTACAG GAGGGGCAGT 
GTACCTGATG AGAATCCACA AGAGGTGCTG CAGAAACCCC TTGCACAGTT AATGTCTAAG 
CTGACGCTAG CCGACATGAA CATCTTGCTG TTTCGCTGCG AAGCAGAAGA ACAAGAGGAC 
GGTGGAGGCT GTTACAGCAT CCCAGGCTGG GAGGGCCTGA AGTATTCAGG ACTACAAGGT 
CTGATATCTG TGTTGGCGGA CATCCGTCCC ACTAATGACC TTGGCCATCC TCTGTGTGCT 
AACCTCAGAG AAGGAGACTG GCTCATAGAC TTCGTCTCCA ACAGGCTGAA GCACAGAGAT 
GGACCACTGG CACAGGTGGG CCAGTGGTTG GGTGCCATGT TTGACTACCT GAGGCACTTC 
CCTCGATACC TCATCCCTTG TTACTTTGAC GCCATCTTGG TGTCAACCTA CACCACAGCT 
CTGGATGCCA CGTTCAAACT CATGTCGAGC TTCATTCAGC GAGGTTCCAC GTTTGTCCGT 
CACCTGGCTC TCGGCTCAGT TCAGATGTGT GGAGTTGGAC GTTTTCCACC TCTGCCTCCC 
ATTTCAACAA AAGTGCAGGA TGTCCCATAC CGCATCAGTC CCATCACTGG GGAAAAGGAG 
CAGTGCTGTG TCTCACTGGC AGCAGGTCTT CCTCATTTTT CTGCTGGGAT TTTCCGTTGT 
TGGGGTAGAG ACACTTTCAT CGCTCTCAGA GGACTCATGC TTATAACAGG GAGACACACT 
GAGGCACGCA ACATCATCCT TGCTTTTGCT GGAACCCTGC GTCACGGTTT GATCCCCAAC 
TTGATGGGAG AGGGTCGCAA TGCCAGATAC AACTGCAGGG ATGCTGTGTG GTGGTGGCTG 
CAGTGCATTC AGGACTATAC CAATCTCGTT CCTGAGGGAC ATGAAATTCT GCGTTGCCCT 
GTCACCCGCA TGTACCCCAC AGACGACTGT GAGCCCTGCA AACCAGGGGA GGTGGAGCAG 
CCATTGTATG ATGTTATCCA GGAGGCACTG CAGCGCCATC TGGAGGGTAT TTCCTTCAGA 
GAGAGGAATG CTGGACCCAA GATGGACAGG AACATGAGAG ATGAAGGGTT CAATGTGGTT 
GCTAAAGTGG ACCCAGCAAC AGGTTTTGTT ACCGGAGGAA ACCGCTTCAA CTGTGGCACT 
TGGATGGATA AAATGGGGGA GAGTGACAGA GCGAGGAACA AGGGGATACC TGCAACTCCA 
AGAGACGGCG CGGCGGTGGA GATAGTTGGT CTGAGTAAAT CAGCAGTTCG TTGGCTTGTG 
GAGCTGCAGT CTAAAGAACT GTTCCCCTAT GATGGCGTTA AGGTGCACAG AGATGGAAAG 
GATGTGTTCA TCTTGTATAC TGAGTGGAAC AAGCAGCTTC AGGAGTCCTT TGAAGTTGAA 
TTCTGGGTGT CTGGGGACTC ACAGGACCCC AATGAGAAGC ACCCTGATTT GGTGCATAAA 
AAGGGAATTT ATAAAGATAG TTATGGAGCT TCCAATCCCT GGTGTGACTA CCAGCTGAGG 
CCCAACTTTA CCATCGCAAT GGTGGTGGCT CCAGAGCTGT TCACGCTAGA AAGAGCATGG 
AAGTCTTTGG AAATAGCTGA AAAGAAACTC CTGGGACCAC TGGGAATGAA AACTCTTGAC 
CCAGATGACA TGGTCTACTG TGGTGTTTAC GATAATGCAC TGGACAACGA CAACTACAAC 
CTGGCCAAGG GCTTTAACTA CCACCAAGGA CCTGAGTGGC TGTGGCCTGT TGGCTATTTT 
CTCCGTGCTA AGCTCTACTT TGCCAAGAGG TTAGGGGAGG AGACCTACTC CAAGACCGTC 
ATCCTAGTGA AAAATGTTCT GTCACGACAT TGCACCCACT TGGAGAGGTC TCTATGGAAA 
GGCTTACCAG AGCTGACCAA TGAAAACGGC CAGCATTGCC CCTTCAGCTG TGAGACCCAG 
GCCTGGTCTC TGGCTACTGT GCTGGAGGTG CTCCATGATC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017165465.1
CDS	155..4819
Translation	MPVSLETRVPSKFRRTSMMLPQHLKQIRVLMLNEKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYADVERLGGGYVVVDPILRVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSYTWEDVGRLVEKLRTEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNESHLNALRRALWEDIFPQIKLWEFYQVNVENAVEKFRVLLQNGTKPDHSKAEGNKGIEIIQDLQCYRYGNRVNMDSALETFVPHSSSPQHIDECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCVVGNIVYERLAGHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWEHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDAAREVRPNLYVIAELFTGSELIDNVFVNRLGINSLIREAMSAGDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIQLRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPISTGEVGFQSGIIAGKLALNKLHQELAAQGFSQVYVDQVDTDIVAVTRHCPSTHQSVVTVCRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREAGSYKKDEKYINGMPEYTVEIKEHVPMVKSAVVKHAGVTSKDRSEFVQEIQFQKLTPGSIIAFRVCLDPKAQKLVGMLRFYLSQFSPKYRRGSVPDENPQEVLQKPLAQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWEGLKYSGLQGLISVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAMFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIQRGSTFVRHLALGSVQMCGVGRFPPLPPISTKVQDVPYRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLMGEGRNARYNCRDAVWWWLQCIQDYTNLVPEGHEILRCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKMDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGIPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVHRDGKDVFILYTEWNKQLQESFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKSLEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKRLGEETYSKTVILVKNVLSRHCTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_017309976.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017309976.1

ORF Insert Sequence:

ATGCCCGTCT CTTTGGAGAC CAGGGTTCCC TCCAAGTTCA GGAGAACTTC CATGATGCTG 
CCTCAGCACC TAAAGCAAAT CAGAGTACTC ATGCTTAACG AAAAGGAGAA TTTAGAGAGA 
ACTTTGTTCA GACTTGAACA AGGTTTTGAG CTCCAGTTCC GCCTGGGTCC AAGCCTTCAA 
GGAAGGAGAG TTATAGTTCA CACCAACTAC CCACTTGATG GACAGAAGTT CATTCGAAAC 
AACTTCCGTG TTTTAGCCTG GAACTACCCA ACAGGAAGGG AGGATGACTC TGATAAGTAC 
TGCTCCCTAG AGCTCAAGAT TGCTGGATCC TACCAGTACT ACTTTGGATA TGCAGATGTG 
GAGAGGTTAG GAGGAGGCTA CGTCGTCGTT GATCCTATTC TTCGTGTTGG AGCAGACAAC 
CATGTCCTCC TGCTGGACTG TGTCACCATC CAGACATACT TGTCCAAATG TCTGGGACGC 
CTTGATGACT GGCCAGACAG ACTGCGAGTA GCCAAGGAGT CAGGTTACAA CATGATCCAC 
TTCACTCCTC TGCAGACTTT GGGAGAGTCT AGGTCCTGTT ACTCCTTAGC TGACCAGCTG 
ACCTTTAGCC TGGAATTCAG CCCAGATGGC AAGAGTTACA CCTGGGAAGA TGTGGGAAGG 
CTAGTGGAAA AACTGAGGAC GGAATGGAAT ATGGTATCAA TCACTGATGT AGTGTATAAT 
CACACTGCTG CAAACAGTGT GTGGATAAAG GAACATCCAG AGTGTGGCTA CAACCTGATG 
AACTCTCCTC ACCTGCGTCC TGCCTGGGTC TTAGACAGAG CTATCTGGCA TTTAACCACT 
AGGGTAGCAG ATGGACGGTA CAAAGAGAAG GGACTTCCTT CTGACATTAC CAACGAGAGC 
CACCTAAATG CTCTCCGCAG GGCGCTTTGG GAGGACATTT TTCCTCAAAT CAAACTTTGG 
GAGTTCTACC AGGTCAACGT GGAAAATGCT GTGGAGAAGT TCAGAGTACT GCTGCAAAAC 
GGCACAAAGC CAGATCATAG CAAGGCTGAA GGAAATAAAG GCATAGAGAT CATTCAAGAC 
CTCCAGTGCT ATCGCTATGG CAACAGAGTG AACATGGACT CAGCTTTGGA GACATTTGTT 
CCTCACAGCT CCTCTCCACA GCACATTGAT GAGTGCTGTG GTTGGCTGAA ACAGAAGTTG 
ACTGAGATGA ATGAAGAACA GTACCACATC ATACACCAGC ATCAGGAACA GGCCGTCAAC 
TGCGTTGTGG GGAACATAGT GTATGAACGT CTAGCTGGCC ACGGTCCTAA ACTGGGTCCT 
GTTACCAGGA AAGAGCCTCT TGTCACCAGG TATTTCACCT TCCCCTTTGA GGAAATGACT 
TTAGAGCAGG AGATGGAGTT TTTAAACCAA CCAGATAAGA TGTGCCACTT CTTGGCTCAC 
AACGGCTGGG TGATGGGTGA TGATCCATTG CGCAACTTTG CTGAACCAGG ATCCAATGTG 
TACCTGCGTC GGGAGCTGAT CTGCTGGGGG GACAGTGTCA AACTCCGCTA TGGAAAAGGA 
CCTGAGGATT GCCCCTACCT GTGGGAGCAC ATGCAGAAAT ACACTGAAAT TACTGCCAAA 
TATTTCCACG GAGTCCGATT GGACAACTGC CACTCTACTC CCTTGCATGT TGCTGAGTTC 
ATGTTAGATG CAGCCAGAGA GGTCAGACCC AACCTGTATG TAATAGCTGA GCTCTTTACA 
GGCAGTGAAC TCATAGACAA TGTGTTTGTT AACCGACTGG GAATCAACAG CCTCATAAGA 
GAGGCCATGT CCGCCGGAGA CAGCCATGAA GAGGGCCGGC TGGTCTATCG TTACGGTGGA 
GAGCCAGTCG GAGCCTTCGT TCAGCCCAGC CTGCGTCCTC TCATGCCTTC TATCGCTCAC 
GCCATGTTCT TGGATGTGAC CCATGACAAT GAGTGTCCCA TTCAGCTGCG GTCAGCTCTC 
GACTCGTTGC CCAGTTCTGC TATCGTTTCC ATGGCCTGCT GTGCTACTGG CTCCACCAGA 
GGATATGATG AACTGGTCCC ACATCAGATA TCTGTGGTGA AGGAGGAGCG TTTTTATCCC 
AAGTGGAATC CCTCTTCAGC ACCCATCTCT ACTGGTGAAG TTGGCTTTCA GAGCGGCATC 
ATCGCTGGGA AACTGGCTCT CAACAAGCTA CACCAGGAGC TGGCTGCCCA GGGCTTTTCA 
CAGGTGTATG TGGACCAGGT TGATACAGAT ATTGTTGCAG TTACTCGTCA CTGCCCCAGC 
ACACACCAGT CTGTGGTAAC AGTGTGTAGG ACAGCCTTCA AGAACCCCAA AACCCATCAG 
TACAGCAACA ATGTCCCACC TATGTTCATA CCTGGCAAGA TTGAAGAAAT TGTACTGGAG 
GCAAGGACAG TGGAGAGAGA GGCTGGGAGT TACAAGAAGG ATGAGAAATA CATAAATGGC 
ATGCCAGAAT ACACAGTGGA AATAAAAGAG CATGTACCGA TGGTGAAAAG TGCAGTGGTG 
AAACATGCTG GAGTGACCTC TAAAGACCGC TCAGAGTTTG TGCAAGAAAT TCAATTCCAG 
AAATTGACTC CTGGTAGCAT CATTGCCTTC AGAGTCTGTT TGGATCCCAA AGCACAGAAG 
CTGGTGGGCA TGTTGAGGTT TTATCTGTCC CAGTTCAGTC CAAAGTACAG GAGGGGCAGT 
GTACCTGATG AGAATCCACA AGAGGTGCTG CAGAAACCCC TTGCACAGTT AATGTCTAAG 
CTGACGCTAG CCGACATGAA CATCTTGCTG TTTCGCTGCG AAGCAGAAGA ACAAGAGGAC 
GGTGGAGGCT GTTACAGCAT CCCAGGCTGG GAGGGCCTGA AGTATTCAGG ACTACAAGGT 
CTGATATCTG TGTTGGCGGA CATCCGTCCC ACTAATGACC TTGGCCATCC TCTGTGTGCT 
AACCTCAGAG AAGGAGACTG GCTCATAGAC TTCGTCTCCA ACAGGCTGAA GCACAGAGAT 
GGACCACTGG CACAGGTGGG CCAGTGGTTG GGTGCCATGT TTGACTACCT GAGGCACTTC 
CCTCGATACC TCATCCCTTG TTACTTTGAC GCCATCTTGG TGTCAACCTA CACCACAGCT 
CTGGATGCCA CGTTCAAACT CATGTCGAGC TTCATTCAGC GAGGTTCCAC GTTTGTCCGT 
CACCTGGCTC TCGGCTCAGT TCAGATGTGT GGAGTTGGAC GTTTTCCACC TCTGCCTCCC 
ATTTCAACAA AAGTGCAGGA TGTCCCATAC CGCATCAGTC CCATCACTGG GGAAAAGGAG 
CAGTGCTGTG TCTCACTGGC AGCAGGTCTT CCTCATTTTT CTGCTGGGAT TTTCCGTTGT 
TGGGGTAGAG ACACTTTCAT CGCTCTCAGA GGACTCATGC TTATAACAGG GAGACACACT 
GAGGCACGCA ACATCATCCT TGCTTTTGCT GGAACCCTGC GTCACGGTTT GATCCCCAAC 
TTGATGGGAG AGGGTCGCAA TGCCAGATAC AACTGCAGGG ATGCTGTGTG GTGGTGGCTG 
CAGTGCATTC AGGACTATAC CAATCTCGTT CCTGAGGGAC ATGAAATTCT GCGTTGCCCT 
GTCACCCGCA TGTACCCCAC AGACGACTGT GAGCCCTGCA AACCAGGGGA GGTGGAGCAG 
CCATTGTATG ATGTTATCCA GGAGGCACTG CAGCGCCATC TGGAGGGTAT TTCCTTCAGA 
GAGAGGAATG CTGGACCCAA GATGGACAGG AACATGAGAG ATGAAGGGTT CAATGTGGTT 
GCTAAAGTGG ACCCAGCAAC AGGTTTTGTT ACCGGAGGAA ACCGCTTCAA CTGTGGCACT 
TGGATGGATA AAATGGGGGA GAGTGACAGA GCGAGGAACA AGGGGATACC TGCAACTCCA 
AGAGACGGCG CGGCGGTGGA GATAGTTGGT CTGAGTAAAT CAGCAGTTCG TTGGCTTGTG 
GAGCTGCAGT CTAAAGAACT GTTCCCCTAT GATGGCGTTA AGGTGCACAG AGATGGAAAG 
GATGTGTTCA TCTTGTATAC TGAGTGGAAC AAGCAGCTTC AGGAGTCCTT TGAAGTTGAA 
TTCTGGGTGT CTGGGGACTC ACAGGACCCC AATGAGAAGC ACCCTGATTT GGTGCATAAA 
AAGGGAATTT ATAAAGATAG TTATGGAGCT TCCAATCCCT GGTGTGACTA CCAGCTGAGG 
CCCAACTTTA CCATCGCAAT GGTGGTGGCT CCAGAGCTGT TCACGCTAGA AAGAGCATGG 
AAGTCTTTGG AAATAGCTGA AAAGAAACTC CTGGGACCAC TGGGAATGAA AACTCTTGAC 
CCAGATGACA TGGTCTACTG TGGTGTTTAC GATAATGCAC TGGACAACGA CAACTACAAC 
CTGGCCAAGG GCTTTAACTA CCACCAAGGA CCTGAGTGGC TGTGGCCTGT TGGCTATTTT 
CTCCGTGCTA AGCTCTACTT TGCCAAGAGG TTAGGGGAGG AGACCTACTC CAAGACCGTC 
ATCCTAGTGA AAAATGTTCT GTCACGACAT TGCACCCACT TGGAGAGGTC TCTATGGAAA 
GGCTTACCAG AGCTGACCAA TGAAAACGGC CAGCATTGCC CCTTCAGCTG TGAGACCCAG 
GCCTGGTCTC TGGCTACTGT GCTGGAGGTG CTCCATGATC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

agl cDNA ORF clone, Poecilia reticulata(guppy)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム