LOC103477581 gene cDNA ORF clone, Poecilia reticulata(guppy)

mRNA	Protein	Name
XM_008430762.2	XP_008428984.1	ras/Rap GTPase-activating protein SynGAP isoform X1
XM_008430765.2	XP_008428987.1	ras/Rap GTPase-activating protein SynGAP isoform X3
XM_008430773.2	XP_008428995.1	ras/Rap GTPase-activating protein SynGAP isoform X10
XM_008430763.2	XP_008428985.1	ras/Rap GTPase-activating protein SynGAP isoform X2
XM_017309407.1	XP_017164896.1	ras/Rap GTPase-activating protein SynGAP isoform X9
XM_008430775.1	XP_008428997.1	ras/Rap GTPase-activating protein SynGAP isoform X11
XM_008430766.2	XP_008428988.1	ras/Rap GTPase-activating protein SynGAP isoform X4
XM_008430768.2	XP_008428990.1	ras/Rap GTPase-activating protein SynGAP isoform X6
XM_017309406.1	XP_017164895.1	ras/Rap GTPase-activating protein SynGAP isoform X8
XM_008430767.2	XP_008428989.1	ras/Rap GTPase-activating protein SynGAP isoform X5
XM_008430770.2	XP_008428992.1	ras/Rap GTPase-activating protein SynGAP isoform X7
XM_008430771.2	XP_008428993.1	ras/Rap GTPase-activating protein SynGAP isoform X9
XM_008430774.2	XP_008428996.1	ras/Rap GTPase-activating protein SynGAP isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPo178215	XM_017309406.1 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo178216	XM_008430771.2 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo178216	XM_017309407.1 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo178217	XM_008430773.2 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo178217	XM_008430774.2 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo178218	XM_008430775.1 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo178208	XM_008430762.2 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPo178209	XM_008430763.2 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPo178210	XM_008430765.2 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPo178211	XM_008430766.2 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPo178212	XM_008430767.2 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPo178213	XM_008430768.2 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPo178214	XM_008430770.2 Latest version!	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

LOC103477581 ( XM_017309406.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017164895.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OPo178215
Clone ID Related Accession (Same CDS sequence)	XM_017309406.1
Accession Version	XM_017309406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACTA CAGATAAAGA TCACCGTGCC CGGAGGAAGA GCATAGCCAC TGGGAAGCAA 
CCCAGCATGG AGGTCCCTCC CACTGCCCCC CTTCAACCCT TCCGACAATC CAGTTTCCTC 
AGTAAGAGGT TGAAGGGCTC CATCAAGAGG GCTAAGAGTC AGCCGAAACT GGACCGAACG 
AGCAGTTTCC GCCAAATGAT CCTCCCCCGC TTCCGCAGTG CCGACCAAGA CAGGACCCGT 
TTGATGCAGA GCTTCAAAGA GTCCCACTCC CATGAGTCCC TGCTGTCTCC CAGCAGCGCT 
GCCGAGGCGC TGGACCTCAC ACTGGACGAG GACGCCATCA TCAAACCCGT CCACTCCAGC 
ATCCTGGGAC AGGAGTACTG TTTTGAGGTG ACAACGGCGT CAGGAACCAA GTGCTTTGCG 
TGTCGCTCAG CCGCAGAGAG AGACAAATGG ATCGAGAACC TGCAGAGAGC TGTCAAGCCC 
AACAAGGACA ACAGCCGGAG GGTGGACAAT GTGCTGAAAC TGTGGATAAT CGAAGCCCGC 
GAGCTCCCGG CTAAGAAGCG CTACTACTGC GAGCTGTGCC TGGACGACAT GCTGTACGCC 
CGCACCACCA GCAAGCCCCG CACCGACACC GTCTTCTGGG GAGAGCACTT TGAGTTCAAC 
AACCTGCCGG CCGTCCGCAA CCTTCGCCTT CATCTGTACA AGGAGACGGA CAAGAAGAGA 
CGGAAGGAAA AGAGCACATA CTTAGGATTG GTCAGCATCC CCATCTCGAG CATCACCGGG 
CGGCAGTTTG TGGAGCAATG GTACCCAGTC ATCCAGCCCA GCGTCCTCAC AAAGGGAGCC 
GGCGTCGGAG GAGGGAAAAT CATCAACGCC TCGCTTCGCC TCAAATCCCG CTTCCAAACC 
ATGAACATCC TGCCCATGGA GCTGTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC 
CGCACCCTGT GCGCCGTGCT GGAGCCCGTG CTGAGCGTGA AGAGTAAAGA GGAAGTGGCC 
TGTGCTTTGG TGCATATCCT GCAAAGCACA GGGAAGGCGA AGGACTTCCT GTCCGACATG 
GCCATGTGTG AGGTAGACAG ATTTATCGAC CGAGAACACC TTATATTCAG AGAGAACACT 
CTGGCCACCA AAGCCATAGA AGAGTACCTC AAACTGATCG GACATAAGTA CCTCAAGGAT 
GCTATCGGGG AGTTCATAAG GGCCTTGTAT GAGTCAGAGG AAAATTGCGA AGTGGACCCT 
ATGAGAATAC CACCCTCTGT GCTACCAGAC CACCAAGCCA ATCTTCGAAT GTGCTGCGAA 
CTGGCTCTCT GCAAAATCGT TAATTCCCAT TGTGTTTTCC CCCGTGAGCT AAAGGAGGTT 
TTTGCGTCCT GGCGAGTGCG TTGTGCCGAG AGGGGTCGGG AAGACATCGC TGACCGCCTC 
ATCAGTGGAT CGCTCTTCCT GCGCTTCCTG TGTCCCGCCA TCATGTCCCC GTCACTTTTC 
AATCTCACCC AGGAGTATCC CGACGAGCAG ACGTCACGCA CGCTCACTCT TATCGCTAAA 
GTAGTGCAAA ACCTCGCAAA CTTCTCCAAG TTTGGCAGTA AGGAGGAGTA CATGTGTTTC 
ATGAATGAGT TTTTAGAGAT GGAGTGGGGC TCCATGCAGC AGTTCCTGTA CGAGATCTCC 
AACCTGGACA GCGTCAGCAA CGCAGGCGGC TTTGAGGGAT ACATCGATCT GGGCAGGGAG 
CTGTCCATCC TGCACAGCCT CCTGTGGGAG GTTATGGCTC AGCTCAGCAA GGATGCCATC 
ATCAAGCTGG GCCCTTTGCC CCGCCTCCTG AATGACATCA GCATGGCGTT GCGTAACCCT 
CACCTCCAGA GGCAGCCGAG CCACCAGACG GACCGGGTGC AGGACAGACA GTCGGACAGG 
CTGCTGTCTC GACCAAGCTT TAACCGAGGA ATCTCGTCAG AGTTCCAGAA TCTCATGATG 
AGGGACTTAA ACAGCTCCAT AGACATCACT CGTTTGCCTT CCCCTACTTC CACCGGAGGG 
GTCATGCCAT CCCGCGCCCA GCTGAGCTTT CAGGACCGCG ATCACCCTCA TCGAGCCTCC 
AAAGACATGT TCTACGTTTC GCGGCCGCCC CTGGCCCGCT CCAGCCCGGC CTACTGCACG 
AGCAGCTCTG ACATCACGGA ACCAGACGCT AAGGATTCCC GAATGAACAG CGTGTCTAAC 
CTGCAGTCGG TGGACATGCT CAACTCCTCC CAGGCCTCCA TCGCCGGTAT GGGCAGCTTC 
GGTGGGCTGA GCAGCGGAGG CGGTGGCGGC CTGGGGTCGG GCCTGAGCAG CCAGCTGCGG 
GCCGGTGGGA GGTTGTCAGC TGGCTCCGGC GGCTCCAGCA TGTCAGGCGG CCTCCGGCTG 
AGCCAGCTGA GCCAGATGGG CACCACCACC GACTCGCTAT CCCAGCAGCA GCAACACCAG 
GCCGCCGCCA TGCGCTACCC GCTTTCCTTC CAGAATCCTC TCTTTCATCT GGCGACGGCT 
GACGGGCCAC AACAACAGCT CCATCACCAG CACAGCCGAG CTCAGCCCCC AGCGCCCCTG 
CTCCTGGCCC CTGACCCAGA GCCCTCGCAC CAGGCCTACA TCCCACAGTT CGCCCATGGG 
GGGTTCTCTC GCAGTGAGGA TCTGTCCACG CTCAGGACCC GGGATGGTCA CCTGGGCCAG 
CCCAGCATAA TCCACTCGCA CAGCTACAGT GACGACTACA GCAGGGCTGA CTATGGACGC 
AGGCAAATCT CCATGCATAT GCAGGATAAC CTCCAACAGC AACACGAAAT GATGGGAATG 
GCCTCCCAGA CTGGAACGTC CCACTCCTCC CTGGCCACCA CTCCTCCCTC CACCGTTCAA 
CCTATGCGGC AGAGCTCTGT TGCTCCGCCT CCCAGCCAGC GCGTCAAGTC CCAGACTTCC 
CACCAGCTAT CCGTCAGCGC GGCGGCCGCG CCCGCCGCCT CTGGTAAAAG CCGTCCACAG 
AGCGGGAACC TCCTCCAGTC TCCAGAGTCT GGCTACGGCG GCAGGCAGCA CGGGCCCCGG 
CAGCTATCGG TCAAAGACAA CACTGCCCCG GGTCTTCCCC ACCAGCAGAG CTCTGTCAGG 
GAAAGCGGGA GTCCACAAGG GACCACCAGT CAGAGCACTC AGCAGTCACC ACAGCAGTCT 
CAACAGCATC TTTTAAAACC CACCATGAGT AAACAGGGCT CCCAGTCACC TACCACCCTC 
AATCCCCCGA CCCCAGCGAA CGAGCGAACA GTGGCCTGGG TCTCCAACAT GCCTCACCTG 
TCGGCCGACA TCGAAAGTTC ACGAATTGAC CGAGAGGAGT TCAAGCTGAA GGAGTATTCG 
AAAAGCATGG ATGAGTCGCG CATGGACAGG GTCAAAGAGT ACGAGGAGGA AATCAACTCT 
TTGAAGGAGC GTCTGCTGAT GTCTCACAGA AAGCTGGAGG AGTACGAGAG GAGGCTCCTA 
GTGCAGGAGC AGCAGACCAA TAAGATCCTC CTCCAGTACC AAAACCGTCT GGAGGACAGC 
GAGAGGAGGC TACGACAACA GCAAGTGGAG AAAGACTCCC AAATTAAAGG AATAATTAAC 
AGACTCATGG CTGTGGAGGA TGAAATAAGG GGAGGAGCCA TTTACGAGCC AAAAACCAGG 
ATTTTCGCTG ATCAGGAGGA CCAGCTTTCC TCCCTGGGTT CAGCAGACCC CGGTGTGAAA 
ACTGAAGGTG GAGGGGGAGG AGGAGGAGGA GGAGGAGAAC AAGGAGGAGC GGTTACCAAC 
GATCAAGGCC CCACAGTCTC TTCCTCTGGC ATTTTCTCCA ACACATCGCC CCACAATGGA 
GTCCTCCCCC AAATTGTAGA CCTTCCCTCC TCACCCACCC CTGATTCACA ACTTAGTCAG 
AATGAAGAGC TCCGTGGCAA TCTAAAGACA ACTTCACAGG CAACTGGAAC CACAGCAACG 
GCAACTACGG GAGTCGCAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017164895.1
CDS	36..4019
Translation	MRTTDKDHRARRKSIATGKQPSMEVPPTAPLQPFRQSSFLSKRLKGSIKRAKSQPKLDRTSSFRQMILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQEDQLSSLGSADPGVKTEGGGGGGGGGGEQGGAVTNDQGPTVSSSGIFSNTSPHNGVLPQIVDLPSSPTPDSQLSQNEELRGNLKTTSQATGTTATATTGVAD

Target ORF information:

RefSeq Version	XM_017309406.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017309406.1

ORF Insert Sequence:

ATGAGGACTA CAGATAAAGA TCACCGTGCC CGGAGGAAGA GCATAGCCAC TGGGAAGCAA 
CCCAGCATGG AGGTCCCTCC CACTGCCCCC CTTCAACCCT TCCGACAATC CAGTTTCCTC 
AGTAAGAGGT TGAAGGGCTC CATCAAGAGG GCTAAGAGTC AGCCGAAACT GGACCGAACG 
AGCAGTTTCC GCCAAATGAT CCTCCCCCGC TTCCGCAGTG CCGACCAAGA CAGGACCCGT 
TTGATGCAGA GCTTCAAAGA GTCCCACTCC CATGAGTCCC TGCTGTCTCC CAGCAGCGCT 
GCCGAGGCGC TGGACCTCAC ACTGGACGAG GACGCCATCA TCAAACCCGT CCACTCCAGC 
ATCCTGGGAC AGGAGTACTG TTTTGAGGTG ACAACGGCGT CAGGAACCAA GTGCTTTGCG 
TGTCGCTCAG CCGCAGAGAG AGACAAATGG ATCGAGAACC TGCAGAGAGC TGTCAAGCCC 
AACAAGGACA ACAGCCGGAG GGTGGACAAT GTGCTGAAAC TGTGGATAAT CGAAGCCCGC 
GAGCTCCCGG CTAAGAAGCG CTACTACTGC GAGCTGTGCC TGGACGACAT GCTGTACGCC 
CGCACCACCA GCAAGCCCCG CACCGACACC GTCTTCTGGG GAGAGCACTT TGAGTTCAAC 
AACCTGCCGG CCGTCCGCAA CCTTCGCCTT CATCTGTACA AGGAGACGGA CAAGAAGAGA 
CGGAAGGAAA AGAGCACATA CTTAGGATTG GTCAGCATCC CCATCTCGAG CATCACCGGG 
CGGCAGTTTG TGGAGCAATG GTACCCAGTC ATCCAGCCCA GCGTCCTCAC AAAGGGAGCC 
GGCGTCGGAG GAGGGAAAAT CATCAACGCC TCGCTTCGCC TCAAATCCCG CTTCCAAACC 
ATGAACATCC TGCCCATGGA GCTGTACAAG GAGTTCGCCG AGTACGTCAC CAACAACTAC 
CGCACCCTGT GCGCCGTGCT GGAGCCCGTG CTGAGCGTGA AGAGTAAAGA GGAAGTGGCC 
TGTGCTTTGG TGCATATCCT GCAAAGCACA GGGAAGGCGA AGGACTTCCT GTCCGACATG 
GCCATGTGTG AGGTAGACAG ATTTATCGAC CGAGAACACC TTATATTCAG AGAGAACACT 
CTGGCCACCA AAGCCATAGA AGAGTACCTC AAACTGATCG GACATAAGTA CCTCAAGGAT 
GCTATCGGGG AGTTCATAAG GGCCTTGTAT GAGTCAGAGG AAAATTGCGA AGTGGACCCT 
ATGAGAATAC CACCCTCTGT GCTACCAGAC CACCAAGCCA ATCTTCGAAT GTGCTGCGAA 
CTGGCTCTCT GCAAAATCGT TAATTCCCAT TGTGTTTTCC CCCGTGAGCT AAAGGAGGTT 
TTTGCGTCCT GGCGAGTGCG TTGTGCCGAG AGGGGTCGGG AAGACATCGC TGACCGCCTC 
ATCAGTGGAT CGCTCTTCCT GCGCTTCCTG TGTCCCGCCA TCATGTCCCC GTCACTTTTC 
AATCTCACCC AGGAGTATCC CGACGAGCAG ACGTCACGCA CGCTCACTCT TATCGCTAAA 
GTAGTGCAAA ACCTCGCAAA CTTCTCCAAG TTTGGCAGTA AGGAGGAGTA CATGTGTTTC 
ATGAATGAGT TTTTAGAGAT GGAGTGGGGC TCCATGCAGC AGTTCCTGTA CGAGATCTCC 
AACCTGGACA GCGTCAGCAA CGCAGGCGGC TTTGAGGGAT ACATCGATCT GGGCAGGGAG 
CTGTCCATCC TGCACAGCCT CCTGTGGGAG GTTATGGCTC AGCTCAGCAA GGATGCCATC 
ATCAAGCTGG GCCCTTTGCC CCGCCTCCTG AATGACATCA GCATGGCGTT GCGTAACCCT 
CACCTCCAGA GGCAGCCGAG CCACCAGACG GACCGGGTGC AGGACAGACA GTCGGACAGG 
CTGCTGTCTC GACCAAGCTT TAACCGAGGA ATCTCGTCAG AGTTCCAGAA TCTCATGATG 
AGGGACTTAA ACAGCTCCAT AGACATCACT CGTTTGCCTT CCCCTACTTC CACCGGAGGG 
GTCATGCCAT CCCGCGCCCA GCTGAGCTTT CAGGACCGCG ATCACCCTCA TCGAGCCTCC 
AAAGACATGT TCTACGTTTC GCGGCCGCCC CTGGCCCGCT CCAGCCCGGC CTACTGCACG 
AGCAGCTCTG ACATCACGGA ACCAGACGCT AAGGATTCCC GAATGAACAG CGTGTCTAAC 
CTGCAGTCGG TGGACATGCT CAACTCCTCC CAGGCCTCCA TCGCCGGTAT GGGCAGCTTC 
GGTGGGCTGA GCAGCGGAGG CGGTGGCGGC CTGGGGTCGG GCCTGAGCAG CCAGCTGCGG 
GCCGGTGGGA GGTTGTCAGC TGGCTCCGGC GGCTCCAGCA TGTCAGGCGG CCTCCGGCTG 
AGCCAGCTGA GCCAGATGGG CACCACCACC GACTCGCTAT CCCAGCAGCA GCAACACCAG 
GCCGCCGCCA TGCGCTACCC GCTTTCCTTC CAGAATCCTC TCTTTCATCT GGCGACGGCT 
GACGGGCCAC AACAACAGCT CCATCACCAG CACAGCCGAG CTCAGCCCCC AGCGCCCCTG 
CTCCTGGCCC CTGACCCAGA GCCCTCGCAC CAGGCCTACA TCCCACAGTT CGCCCATGGG 
GGGTTCTCTC GCAGTGAGGA TCTGTCCACG CTCAGGACCC GGGATGGTCA CCTGGGCCAG 
CCCAGCATAA TCCACTCGCA CAGCTACAGT GACGACTACA GCAGGGCTGA CTATGGACGC 
AGGCAAATCT CCATGCATAT GCAGGATAAC CTCCAACAGC AACACGAAAT GATGGGAATG 
GCCTCCCAGA CTGGAACGTC CCACTCCTCC CTGGCCACCA CTCCTCCCTC CACCGTTCAA 
CCTATGCGGC AGAGCTCTGT TGCTCCGCCT CCCAGCCAGC GCGTCAAGTC CCAGACTTCC 
CACCAGCTAT CCGTCAGCGC GGCGGCCGCG CCCGCCGCCT CTGGTAAAAG CCGTCCACAG 
AGCGGGAACC TCCTCCAGTC TCCAGAGTCT GGCTACGGCG GCAGGCAGCA CGGGCCCCGG 
CAGCTATCGG TCAAAGACAA CACTGCCCCG GGTCTTCCCC ACCAGCAGAG CTCTGTCAGG 
GAAAGCGGGA GTCCACAAGG GACCACCAGT CAGAGCACTC AGCAGTCACC ACAGCAGTCT 
CAACAGCATC TTTTAAAACC CACCATGAGT AAACAGGGCT CCCAGTCACC TACCACCCTC 
AATCCCCCGA CCCCAGCGAA CGAGCGAACA GTGGCCTGGG TCTCCAACAT GCCTCACCTG 
TCGGCCGACA TCGAAAGTTC ACGAATTGAC CGAGAGGAGT TCAAGCTGAA GGAGTATTCG 
AAAAGCATGG ATGAGTCGCG CATGGACAGG GTCAAAGAGT ACGAGGAGGA AATCAACTCT 
TTGAAGGAGC GTCTGCTGAT GTCTCACAGA AAGCTGGAGG AGTACGAGAG GAGGCTCCTA 
GTGCAGGAGC AGCAGACCAA TAAGATCCTC CTCCAGTACC AAAACCGTCT GGAGGACAGC 
GAGAGGAGGC TACGACAACA GCAAGTGGAG AAAGACTCCC AAATTAAAGG AATAATTAAC 
AGACTCATGG CTGTGGAGGA TGAAATAAGG GGAGGAGCCA TTTACGAGCC AAAAACCAGG 
ATTTTCGCTG ATCAGGAGGA CCAGCTTTCC TCCCTGGGTT CAGCAGACCC CGGTGTGAAA 
ACTGAAGGTG GAGGGGGAGG AGGAGGAGGA GGAGGAGAAC AAGGAGGAGC GGTTACCAAC 
GATCAAGGCC CCACAGTCTC TTCCTCTGGC ATTTTCTCCA ACACATCGCC CCACAATGGA 
GTCCTCCCCC AAATTGTAGA CCTTCCCTCC TCACCCACCC CTGATTCACA ACTTAGTCAG 
AATGAAGAGC TCCGTGGCAA TCTAAAGACA ACTTCACAGG CAACTGGAAC CACAGCAACG 
GCAACTACGG GAGTCGCAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_017309407.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017164896.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OPo178216
Clone ID Related Accession (Same CDS sequence)	XM_017309407.1 , XM_008430771.2
Accession Version	XM_017309407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG AGGACCAGCT TTCCTCCCTG GGTTCAGCAG ACCCCGGTGT GAAAACTGAA 
GGTGGAGGGG GAGGAGGAGG AGGAGGAGGA GAACAAGGAG GAGCGGTTAC CAACGATCAA 
GGCCCCACAG TCTCTTCCTC TGGCATTTTC TCCAACACAT CGCCCCACAA TGGAGTCCTC 
CCCCAAATTG TAGACCTTCC CTCCTCACCC ACCCCTGATT CACAACTTAG TCAGAATGAA 
GAGCTCCGTG GCAATCTAAA GACAACTTCA CAGGCAACTG GAACCACAGC AACGGCAACT 
ACGGGAGTCG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017164896.1
CDS	181..4098
Translation	MEVPPTAPLQPFRQSSFLSKRLKGSIKRAKSQPKLDRTSSFRQMILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQEDQLSSLGSADPGVKTEGGGGGGGGGGEQGGAVTNDQGPTVSSSGIFSNTSPHNGVLPQIVDLPSSPTPDSQLSQNEELRGNLKTTSQATGTTATATTGVAD

Target ORF information:

RefSeq Version	XM_017309407.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017309407.1

ORF Insert Sequence:

ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG AGGACCAGCT TTCCTCCCTG GGTTCAGCAG ACCCCGGTGT GAAAACTGAA 
GGTGGAGGGG GAGGAGGAGG AGGAGGAGGA GAACAAGGAG GAGCGGTTAC CAACGATCAA 
GGCCCCACAG TCTCTTCCTC TGGCATTTTC TCCAACACAT CGCCCCACAA TGGAGTCCTC 
CCCCAAATTG TAGACCTTCC CTCCTCACCC ACCCCTGATT CACAACTTAG TCAGAATGAA 
GAGCTCCGTG GCAATCTAAA GACAACTTCA CAGGCAACTG GAACCACAGC AACGGCAACT 
ACGGGAGTCG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430771.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428993.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X12, mRNA.

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CloneID	OPo178216
Clone ID Related Accession (Same CDS sequence)	XM_017309407.1 , XM_008430771.2
Accession Version	XM_008430771.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008430771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG AGGACCAGCT TTCCTCCCTG GGTTCAGCAG ACCCCGGTGT GAAAACTGAA 
GGTGGAGGGG GAGGAGGAGG AGGAGGAGGA GAACAAGGAG GAGCGGTTAC CAACGATCAA 
GGCCCCACAG TCTCTTCCTC TGGCATTTTC TCCAACACAT CGCCCCACAA TGGAGTCCTC 
CCCCAAATTG TAGACCTTCC CTCCTCACCC ACCCCTGATT CACAACTTAG TCAGAATGAA 
GAGCTCCGTG GCAATCTAAA GACAACTTCA CAGGCAACTG GAACCACAGC AACGGCAACT 
ACGGGAGTCG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428993.1
CDS	1916..5833
Translation	MEVPPTAPLQPFRQSSFLSKRLKGSIKRAKSQPKLDRTSSFRQMILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQEDQLSSLGSADPGVKTEGGGGGGGGGGEQGGAVTNDQGPTVSSSGIFSNTSPHNGVLPQIVDLPSSPTPDSQLSQNEELRGNLKTTSQATGTTATATTGVAD

Target ORF information:

RefSeq Version	XM_008430771.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430771.2

ORF Insert Sequence:

ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG AGGACCAGCT TTCCTCCCTG GGTTCAGCAG ACCCCGGTGT GAAAACTGAA 
GGTGGAGGGG GAGGAGGAGG AGGAGGAGGA GAACAAGGAG GAGCGGTTAC CAACGATCAA 
GGCCCCACAG TCTCTTCCTC TGGCATTTTC TCCAACACAT CGCCCCACAA TGGAGTCCTC 
CCCCAAATTG TAGACCTTCC CTCCTCACCC ACCCCTGATT CACAACTTAG TCAGAATGAA 
GAGCTCCGTG GCAATCTAAA GACAACTTCA CAGGCAACTG GAACCACAGC AACGGCAACT 
ACGGGAGTCG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430773.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428995.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OPo178217
Clone ID Related Accession (Same CDS sequence)	XM_008430773.2 , XM_008430774.2
Accession Version	XM_008430773.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008430773.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGCT TCAAAGAGTC CCACTCCCAT GAGTCCCTGC TGTCTCCCAG CAGCGCTGCC 
GAGGCGCTGG ACCTCACACT GGACGAGGAC GCCATCATCA AACCCGTCCA CTCCAGCATC 
CTGGGACAGG AGTACTGTTT TGAGGTGACA ACGGCGTCAG GAACCAAGTG CTTTGCGTGT 
CGCTCAGCCG CAGAGAGAGA CAAATGGATC GAGAACCTGC AGAGAGCTGT CAAGCCCAAC 
AAGGACAACA GCCGGAGGGT GGACAATGTG CTGAAACTGT GGATAATCGA AGCCCGCGAG 
CTCCCGGCTA AGAAGCGCTA CTACTGCGAG CTGTGCCTGG ACGACATGCT GTACGCCCGC 
ACCACCAGCA AGCCCCGCAC CGACACCGTC TTCTGGGGAG AGCACTTTGA GTTCAACAAC 
CTGCCGGCCG TCCGCAACCT TCGCCTTCAT CTGTACAAGG AGACGGACAA GAAGAGACGG 
AAGGAAAAGA GCACATACTT AGGATTGGTC AGCATCCCCA TCTCGAGCAT CACCGGGCGG 
CAGTTTGTGG AGCAATGGTA CCCAGTCATC CAGCCCAGCG TCCTCACAAA GGGAGCCGGC 
GTCGGAGGAG GGAAAATCAT CAACGCCTCG CTTCGCCTCA AATCCCGCTT CCAAACCATG 
AACATCCTGC CCATGGAGCT GTACAAGGAG TTCGCCGAGT ACGTCACCAA CAACTACCGC 
ACCCTGTGCG CCGTGCTGGA GCCCGTGCTG AGCGTGAAGA GTAAAGAGGA AGTGGCCTGT 
GCTTTGGTGC ATATCCTGCA AAGCACAGGG AAGGCGAAGG ACTTCCTGTC CGACATGGCC 
ATGTGTGAGG TAGACAGATT TATCGACCGA GAACACCTTA TATTCAGAGA GAACACTCTG 
GCCACCAAAG CCATAGAAGA GTACCTCAAA CTGATCGGAC ATAAGTACCT CAAGGATGCT 
ATCGGGGAGT TCATAAGGGC CTTGTATGAG TCAGAGGAAA ATTGCGAAGT GGACCCTATG 
AGAATACCAC CCTCTGTGCT ACCAGACCAC CAAGCCAATC TTCGAATGTG CTGCGAACTG 
GCTCTCTGCA AAATCGTTAA TTCCCATTGT GTTTTCCCCC GTGAGCTAAA GGAGGTTTTT 
GCGTCCTGGC GAGTGCGTTG TGCCGAGAGG GGTCGGGAAG ACATCGCTGA CCGCCTCATC 
AGTGGATCGC TCTTCCTGCG CTTCCTGTGT CCCGCCATCA TGTCCCCGTC ACTTTTCAAT 
CTCACCCAGG AGTATCCCGA CGAGCAGACG TCACGCACGC TCACTCTTAT CGCTAAAGTA 
GTGCAAAACC TCGCAAACTT CTCCAAGTTT GGCAGTAAGG AGGAGTACAT GTGTTTCATG 
AATGAGTTTT TAGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTGTACGA GATCTCCAAC 
CTGGACAGCG TCAGCAACGC AGGCGGCTTT GAGGGATACA TCGATCTGGG CAGGGAGCTG 
TCCATCCTGC ACAGCCTCCT GTGGGAGGTT ATGGCTCAGC TCAGCAAGGA TGCCATCATC 
AAGCTGGGCC CTTTGCCCCG CCTCCTGAAT GACATCAGCA TGGCGTTGCG TAACCCTCAC 
CTCCAGAGGC AGCCGAGCCA CCAGACGGAC CGGGTGCAGG ACAGACAGTC GGACAGGCTG 
CTGTCTCGAC CAAGCTTTAA CCGAGGAATC TCGTCAGAGT TCCAGAATCT CATGATGAGG 
GACTTAAACA GCTCCATAGA CATCACTCGT TTGCCTTCCC CTACTTCCAC CGGAGGGGTC 
ATGCCATCCC GCGCCCAGCT GAGCTTTCAG GACCGCGATC ACCCTCATCG AGCCTCCAAA 
GACATGTTCT ACGTTTCGCG GCCGCCCCTG GCCCGCTCCA GCCCGGCCTA CTGCACGAGC 
AGCTCTGACA TCACGGAACC AGACGCTAAG GATTCCCGAA TGAACAGCGT GTCTAACCTG 
CAGTCGGTGG ACATGCTCAA CTCCTCCCAG GCCTCCATCG CCGGTATGGG CAGCTTCGGT 
GGGCTGAGCA GCGGAGGCGG TGGCGGCCTG GGGTCGGGCC TGAGCAGCCA GCTGCGGGCC 
GGTGGGAGGT TGTCAGCTGG CTCCGGCGGC TCCAGCATGT CAGGCGGCCT CCGGCTGAGC 
CAGCTGAGCC AGATGGGCAC CACCACCGAC TCGCTATCCC AGCAGCAGCA ACACCAGGCC 
GCCGCCATGC GCTACCCGCT TTCCTTCCAG AATCCTCTCT TTCATCTGGC GACGGCTGAC 
GGGCCACAAC AACAGCTCCA TCACCAGCAC AGCCGAGCTC AGCCCCCAGC GCCCCTGCTC 
CTGGCCCCTG ACCCAGAGCC CTCGCACCAG GCCTACATCC CACAGTTCGC CCATGGGGGG 
TTCTCTCGCA GTGAGGATCT GTCCACGCTC AGGACCCGGG ATGGTCACCT GGGCCAGCCC 
AGCATAATCC ACTCGCACAG CTACAGTGAC GACTACAGCA GGGCTGACTA TGGACGCAGG 
CAAATCTCCA TGCATATGCA GGATAACCTC CAACAGCAAC ACGAAATGAT GGGAATGGCC 
TCCCAGACTG GAACGTCCCA CTCCTCCCTG GCCACCACTC CTCCCTCCAC CGTTCAACCT 
ATGCGGCAGA GCTCTGTTGC TCCGCCTCCC AGCCAGCGCG TCAAGTCCCA GACTTCCCAC 
CAGCTATCCG TCAGCGCGGC GGCCGCGCCC GCCGCCTCTG GTAAAAGCCG TCCACAGAGC 
GGGAACCTCC TCCAGTCTCC AGAGTCTGGC TACGGCGGCA GGCAGCACGG GCCCCGGCAG 
CTATCGGTCA AAGACAACAC TGCCCCGGGT CTTCCCCACC AGCAGAGCTC TGTCAGGGAA 
AGCGGGAGTC CACAAGGGAC CACCAGTCAG AGCACTCAGC AGTCACCACA GCAGTCTCAA 
CAGCATCTTT TAAAACCCAC CATGAGTAAA CAGGGCTCCC AGTCACCTAC CACCCTCAAT 
CCCCCGACCC CAGCGAACGA GCGAACAGTG GCCTGGGTCT CCAACATGCC TCACCTGTCG 
GCCGACATCG AAAGTTCACG AATTGACCGA GAGGAGTTCA AGCTGAAGGA GTATTCGAAA 
AGCATGGATG AGTCGCGCAT GGACAGGGTC AAAGAGTACG AGGAGGAAAT CAACTCTTTG 
AAGGAGCGTC TGCTGATGTC TCACAGAAAG CTGGAGGAGT ACGAGAGGAG GCTCCTAGTG 
CAGGAGCAGC AGACCAATAA GATCCTCCTC CAGTACCAAA ACCGTCTGGA GGACAGCGAG 
AGGAGGCTAC GACAACAGCA AGTGGAGAAA GACTCCCAAA TTAAAGGAAT AATTAACAGA 
CTCATGGCTG TGGAGGATGA AATAAGGGGA GGAGCCATTT ACGAGCCAAA AACCAGGATT 
TTCGCTGATC AGGAGGACCA GCTTTCCTCC CTGGGTTCAG CAGACCCCGG TGTGAAAACT 
GAAGGTGGAG GGGGAGGAGG AGGAGGAGGA GGAGAACAAG GAGGAGCGGT TACCAACGAT 
CAAGGCCCCA CAGTCTCTTC CTCTGGCATT TTCTCCAACA CATCGCCCCA CAATGGAGTC 
CTCCCCCAAA TTGTAGACCT TCCCTCCTCA CCCACCCCTG ATTCACAACT TAGTCAGAAT 
GAAGAGCTCC GTGGCAATCT AAAGACAACT TCACAGGCAA CTGGAACCAC AGCAACGGCA 
ACTACGGGAG TCGCAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428995.1
CDS	34..3774
Translation	MQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQEDQLSSLGSADPGVKTEGGGGGGGGGGEQGGAVTNDQGPTVSSSGIFSNTSPHNGVLPQIVDLPSSPTPDSQLSQNEELRGNLKTTSQATGTTATATTGVAD

Target ORF information:

RefSeq Version	XM_008430773.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430773.2

ORF Insert Sequence:

ATGCAGAGCT TCAAAGAGTC CCACTCCCAT GAGTCCCTGC TGTCTCCCAG CAGCGCTGCC 
GAGGCGCTGG ACCTCACACT GGACGAGGAC GCCATCATCA AACCCGTCCA CTCCAGCATC 
CTGGGACAGG AGTACTGTTT TGAGGTGACA ACGGCGTCAG GAACCAAGTG CTTTGCGTGT 
CGCTCAGCCG CAGAGAGAGA CAAATGGATC GAGAACCTGC AGAGAGCTGT CAAGCCCAAC 
AAGGACAACA GCCGGAGGGT GGACAATGTG CTGAAACTGT GGATAATCGA AGCCCGCGAG 
CTCCCGGCTA AGAAGCGCTA CTACTGCGAG CTGTGCCTGG ACGACATGCT GTACGCCCGC 
ACCACCAGCA AGCCCCGCAC CGACACCGTC TTCTGGGGAG AGCACTTTGA GTTCAACAAC 
CTGCCGGCCG TCCGCAACCT TCGCCTTCAT CTGTACAAGG AGACGGACAA GAAGAGACGG 
AAGGAAAAGA GCACATACTT AGGATTGGTC AGCATCCCCA TCTCGAGCAT CACCGGGCGG 
CAGTTTGTGG AGCAATGGTA CCCAGTCATC CAGCCCAGCG TCCTCACAAA GGGAGCCGGC 
GTCGGAGGAG GGAAAATCAT CAACGCCTCG CTTCGCCTCA AATCCCGCTT CCAAACCATG 
AACATCCTGC CCATGGAGCT GTACAAGGAG TTCGCCGAGT ACGTCACCAA CAACTACCGC 
ACCCTGTGCG CCGTGCTGGA GCCCGTGCTG AGCGTGAAGA GTAAAGAGGA AGTGGCCTGT 
GCTTTGGTGC ATATCCTGCA AAGCACAGGG AAGGCGAAGG ACTTCCTGTC CGACATGGCC 
ATGTGTGAGG TAGACAGATT TATCGACCGA GAACACCTTA TATTCAGAGA GAACACTCTG 
GCCACCAAAG CCATAGAAGA GTACCTCAAA CTGATCGGAC ATAAGTACCT CAAGGATGCT 
ATCGGGGAGT TCATAAGGGC CTTGTATGAG TCAGAGGAAA ATTGCGAAGT GGACCCTATG 
AGAATACCAC CCTCTGTGCT ACCAGACCAC CAAGCCAATC TTCGAATGTG CTGCGAACTG 
GCTCTCTGCA AAATCGTTAA TTCCCATTGT GTTTTCCCCC GTGAGCTAAA GGAGGTTTTT 
GCGTCCTGGC GAGTGCGTTG TGCCGAGAGG GGTCGGGAAG ACATCGCTGA CCGCCTCATC 
AGTGGATCGC TCTTCCTGCG CTTCCTGTGT CCCGCCATCA TGTCCCCGTC ACTTTTCAAT 
CTCACCCAGG AGTATCCCGA CGAGCAGACG TCACGCACGC TCACTCTTAT CGCTAAAGTA 
GTGCAAAACC TCGCAAACTT CTCCAAGTTT GGCAGTAAGG AGGAGTACAT GTGTTTCATG 
AATGAGTTTT TAGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTGTACGA GATCTCCAAC 
CTGGACAGCG TCAGCAACGC AGGCGGCTTT GAGGGATACA TCGATCTGGG CAGGGAGCTG 
TCCATCCTGC ACAGCCTCCT GTGGGAGGTT ATGGCTCAGC TCAGCAAGGA TGCCATCATC 
AAGCTGGGCC CTTTGCCCCG CCTCCTGAAT GACATCAGCA TGGCGTTGCG TAACCCTCAC 
CTCCAGAGGC AGCCGAGCCA CCAGACGGAC CGGGTGCAGG ACAGACAGTC GGACAGGCTG 
CTGTCTCGAC CAAGCTTTAA CCGAGGAATC TCGTCAGAGT TCCAGAATCT CATGATGAGG 
GACTTAAACA GCTCCATAGA CATCACTCGT TTGCCTTCCC CTACTTCCAC CGGAGGGGTC 
ATGCCATCCC GCGCCCAGCT GAGCTTTCAG GACCGCGATC ACCCTCATCG AGCCTCCAAA 
GACATGTTCT ACGTTTCGCG GCCGCCCCTG GCCCGCTCCA GCCCGGCCTA CTGCACGAGC 
AGCTCTGACA TCACGGAACC AGACGCTAAG GATTCCCGAA TGAACAGCGT GTCTAACCTG 
CAGTCGGTGG ACATGCTCAA CTCCTCCCAG GCCTCCATCG CCGGTATGGG CAGCTTCGGT 
GGGCTGAGCA GCGGAGGCGG TGGCGGCCTG GGGTCGGGCC TGAGCAGCCA GCTGCGGGCC 
GGTGGGAGGT TGTCAGCTGG CTCCGGCGGC TCCAGCATGT CAGGCGGCCT CCGGCTGAGC 
CAGCTGAGCC AGATGGGCAC CACCACCGAC TCGCTATCCC AGCAGCAGCA ACACCAGGCC 
GCCGCCATGC GCTACCCGCT TTCCTTCCAG AATCCTCTCT TTCATCTGGC GACGGCTGAC 
GGGCCACAAC AACAGCTCCA TCACCAGCAC AGCCGAGCTC AGCCCCCAGC GCCCCTGCTC 
CTGGCCCCTG ACCCAGAGCC CTCGCACCAG GCCTACATCC CACAGTTCGC CCATGGGGGG 
TTCTCTCGCA GTGAGGATCT GTCCACGCTC AGGACCCGGG ATGGTCACCT GGGCCAGCCC 
AGCATAATCC ACTCGCACAG CTACAGTGAC GACTACAGCA GGGCTGACTA TGGACGCAGG 
CAAATCTCCA TGCATATGCA GGATAACCTC CAACAGCAAC ACGAAATGAT GGGAATGGCC 
TCCCAGACTG GAACGTCCCA CTCCTCCCTG GCCACCACTC CTCCCTCCAC CGTTCAACCT 
ATGCGGCAGA GCTCTGTTGC TCCGCCTCCC AGCCAGCGCG TCAAGTCCCA GACTTCCCAC 
CAGCTATCCG TCAGCGCGGC GGCCGCGCCC GCCGCCTCTG GTAAAAGCCG TCCACAGAGC 
GGGAACCTCC TCCAGTCTCC AGAGTCTGGC TACGGCGGCA GGCAGCACGG GCCCCGGCAG 
CTATCGGTCA AAGACAACAC TGCCCCGGGT CTTCCCCACC AGCAGAGCTC TGTCAGGGAA 
AGCGGGAGTC CACAAGGGAC CACCAGTCAG AGCACTCAGC AGTCACCACA GCAGTCTCAA 
CAGCATCTTT TAAAACCCAC CATGAGTAAA CAGGGCTCCC AGTCACCTAC CACCCTCAAT 
CCCCCGACCC CAGCGAACGA GCGAACAGTG GCCTGGGTCT CCAACATGCC TCACCTGTCG 
GCCGACATCG AAAGTTCACG AATTGACCGA GAGGAGTTCA AGCTGAAGGA GTATTCGAAA 
AGCATGGATG AGTCGCGCAT GGACAGGGTC AAAGAGTACG AGGAGGAAAT CAACTCTTTG 
AAGGAGCGTC TGCTGATGTC TCACAGAAAG CTGGAGGAGT ACGAGAGGAG GCTCCTAGTG 
CAGGAGCAGC AGACCAATAA GATCCTCCTC CAGTACCAAA ACCGTCTGGA GGACAGCGAG 
AGGAGGCTAC GACAACAGCA AGTGGAGAAA GACTCCCAAA TTAAAGGAAT AATTAACAGA 
CTCATGGCTG TGGAGGATGA AATAAGGGGA GGAGCCATTT ACGAGCCAAA AACCAGGATT 
TTCGCTGATC AGGAGGACCA GCTTTCCTCC CTGGGTTCAG CAGACCCCGG TGTGAAAACT 
GAAGGTGGAG GGGGAGGAGG AGGAGGAGGA GGAGAACAAG GAGGAGCGGT TACCAACGAT 
CAAGGCCCCA CAGTCTCTTC CTCTGGCATT TTCTCCAACA CATCGCCCCA CAATGGAGTC 
CTCCCCCAAA TTGTAGACCT TCCCTCCTCA CCCACCCCTG ATTCACAACT TAGTCAGAAT 
GAAGAGCTCC GTGGCAATCT AAAGACAACT TCACAGGCAA CTGGAACCAC AGCAACGGCA 
ACTACGGGAG TCGCAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430774.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428996.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OPo178217
Clone ID Related Accession (Same CDS sequence)	XM_008430773.2 , XM_008430774.2
Accession Version	XM_008430774.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008430774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGCT TCAAAGAGTC CCACTCCCAT GAGTCCCTGC TGTCTCCCAG CAGCGCTGCC 
GAGGCGCTGG ACCTCACACT GGACGAGGAC GCCATCATCA AACCCGTCCA CTCCAGCATC 
CTGGGACAGG AGTACTGTTT TGAGGTGACA ACGGCGTCAG GAACCAAGTG CTTTGCGTGT 
CGCTCAGCCG CAGAGAGAGA CAAATGGATC GAGAACCTGC AGAGAGCTGT CAAGCCCAAC 
AAGGACAACA GCCGGAGGGT GGACAATGTG CTGAAACTGT GGATAATCGA AGCCCGCGAG 
CTCCCGGCTA AGAAGCGCTA CTACTGCGAG CTGTGCCTGG ACGACATGCT GTACGCCCGC 
ACCACCAGCA AGCCCCGCAC CGACACCGTC TTCTGGGGAG AGCACTTTGA GTTCAACAAC 
CTGCCGGCCG TCCGCAACCT TCGCCTTCAT CTGTACAAGG AGACGGACAA GAAGAGACGG 
AAGGAAAAGA GCACATACTT AGGATTGGTC AGCATCCCCA TCTCGAGCAT CACCGGGCGG 
CAGTTTGTGG AGCAATGGTA CCCAGTCATC CAGCCCAGCG TCCTCACAAA GGGAGCCGGC 
GTCGGAGGAG GGAAAATCAT CAACGCCTCG CTTCGCCTCA AATCCCGCTT CCAAACCATG 
AACATCCTGC CCATGGAGCT GTACAAGGAG TTCGCCGAGT ACGTCACCAA CAACTACCGC 
ACCCTGTGCG CCGTGCTGGA GCCCGTGCTG AGCGTGAAGA GTAAAGAGGA AGTGGCCTGT 
GCTTTGGTGC ATATCCTGCA AAGCACAGGG AAGGCGAAGG ACTTCCTGTC CGACATGGCC 
ATGTGTGAGG TAGACAGATT TATCGACCGA GAACACCTTA TATTCAGAGA GAACACTCTG 
GCCACCAAAG CCATAGAAGA GTACCTCAAA CTGATCGGAC ATAAGTACCT CAAGGATGCT 
ATCGGGGAGT TCATAAGGGC CTTGTATGAG TCAGAGGAAA ATTGCGAAGT GGACCCTATG 
AGAATACCAC CCTCTGTGCT ACCAGACCAC CAAGCCAATC TTCGAATGTG CTGCGAACTG 
GCTCTCTGCA AAATCGTTAA TTCCCATTGT GTTTTCCCCC GTGAGCTAAA GGAGGTTTTT 
GCGTCCTGGC GAGTGCGTTG TGCCGAGAGG GGTCGGGAAG ACATCGCTGA CCGCCTCATC 
AGTGGATCGC TCTTCCTGCG CTTCCTGTGT CCCGCCATCA TGTCCCCGTC ACTTTTCAAT 
CTCACCCAGG AGTATCCCGA CGAGCAGACG TCACGCACGC TCACTCTTAT CGCTAAAGTA 
GTGCAAAACC TCGCAAACTT CTCCAAGTTT GGCAGTAAGG AGGAGTACAT GTGTTTCATG 
AATGAGTTTT TAGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTGTACGA GATCTCCAAC 
CTGGACAGCG TCAGCAACGC AGGCGGCTTT GAGGGATACA TCGATCTGGG CAGGGAGCTG 
TCCATCCTGC ACAGCCTCCT GTGGGAGGTT ATGGCTCAGC TCAGCAAGGA TGCCATCATC 
AAGCTGGGCC CTTTGCCCCG CCTCCTGAAT GACATCAGCA TGGCGTTGCG TAACCCTCAC 
CTCCAGAGGC AGCCGAGCCA CCAGACGGAC CGGGTGCAGG ACAGACAGTC GGACAGGCTG 
CTGTCTCGAC CAAGCTTTAA CCGAGGAATC TCGTCAGAGT TCCAGAATCT CATGATGAGG 
GACTTAAACA GCTCCATAGA CATCACTCGT TTGCCTTCCC CTACTTCCAC CGGAGGGGTC 
ATGCCATCCC GCGCCCAGCT GAGCTTTCAG GACCGCGATC ACCCTCATCG AGCCTCCAAA 
GACATGTTCT ACGTTTCGCG GCCGCCCCTG GCCCGCTCCA GCCCGGCCTA CTGCACGAGC 
AGCTCTGACA TCACGGAACC AGACGCTAAG GATTCCCGAA TGAACAGCGT GTCTAACCTG 
CAGTCGGTGG ACATGCTCAA CTCCTCCCAG GCCTCCATCG CCGGTATGGG CAGCTTCGGT 
GGGCTGAGCA GCGGAGGCGG TGGCGGCCTG GGGTCGGGCC TGAGCAGCCA GCTGCGGGCC 
GGTGGGAGGT TGTCAGCTGG CTCCGGCGGC TCCAGCATGT CAGGCGGCCT CCGGCTGAGC 
CAGCTGAGCC AGATGGGCAC CACCACCGAC TCGCTATCCC AGCAGCAGCA ACACCAGGCC 
GCCGCCATGC GCTACCCGCT TTCCTTCCAG AATCCTCTCT TTCATCTGGC GACGGCTGAC 
GGGCCACAAC AACAGCTCCA TCACCAGCAC AGCCGAGCTC AGCCCCCAGC GCCCCTGCTC 
CTGGCCCCTG ACCCAGAGCC CTCGCACCAG GCCTACATCC CACAGTTCGC CCATGGGGGG 
TTCTCTCGCA GTGAGGATCT GTCCACGCTC AGGACCCGGG ATGGTCACCT GGGCCAGCCC 
AGCATAATCC ACTCGCACAG CTACAGTGAC GACTACAGCA GGGCTGACTA TGGACGCAGG 
CAAATCTCCA TGCATATGCA GGATAACCTC CAACAGCAAC ACGAAATGAT GGGAATGGCC 
TCCCAGACTG GAACGTCCCA CTCCTCCCTG GCCACCACTC CTCCCTCCAC CGTTCAACCT 
ATGCGGCAGA GCTCTGTTGC TCCGCCTCCC AGCCAGCGCG TCAAGTCCCA GACTTCCCAC 
CAGCTATCCG TCAGCGCGGC GGCCGCGCCC GCCGCCTCTG GTAAAAGCCG TCCACAGAGC 
GGGAACCTCC TCCAGTCTCC AGAGTCTGGC TACGGCGGCA GGCAGCACGG GCCCCGGCAG 
CTATCGGTCA AAGACAACAC TGCCCCGGGT CTTCCCCACC AGCAGAGCTC TGTCAGGGAA 
AGCGGGAGTC CACAAGGGAC CACCAGTCAG AGCACTCAGC AGTCACCACA GCAGTCTCAA 
CAGCATCTTT TAAAACCCAC CATGAGTAAA CAGGGCTCCC AGTCACCTAC CACCCTCAAT 
CCCCCGACCC CAGCGAACGA GCGAACAGTG GCCTGGGTCT CCAACATGCC TCACCTGTCG 
GCCGACATCG AAAGTTCACG AATTGACCGA GAGGAGTTCA AGCTGAAGGA GTATTCGAAA 
AGCATGGATG AGTCGCGCAT GGACAGGGTC AAAGAGTACG AGGAGGAAAT CAACTCTTTG 
AAGGAGCGTC TGCTGATGTC TCACAGAAAG CTGGAGGAGT ACGAGAGGAG GCTCCTAGTG 
CAGGAGCAGC AGACCAATAA GATCCTCCTC CAGTACCAAA ACCGTCTGGA GGACAGCGAG 
AGGAGGCTAC GACAACAGCA AGTGGAGAAA GACTCCCAAA TTAAAGGAAT AATTAACAGA 
CTCATGGCTG TGGAGGATGA AATAAGGGGA GGAGCCATTT ACGAGCCAAA AACCAGGATT 
TTCGCTGATC AGGAGGACCA GCTTTCCTCC CTGGGTTCAG CAGACCCCGG TGTGAAAACT 
GAAGGTGGAG GGGGAGGAGG AGGAGGAGGA GGAGAACAAG GAGGAGCGGT TACCAACGAT 
CAAGGCCCCA CAGTCTCTTC CTCTGGCATT TTCTCCAACA CATCGCCCCA CAATGGAGTC 
CTCCCCCAAA TTGTAGACCT TCCCTCCTCA CCCACCCCTG ATTCACAACT TAGTCAGAAT 
GAAGAGCTCC GTGGCAATCT AAAGACAACT TCACAGGCAA CTGGAACCAC AGCAACGGCA 
ACTACGGGAG TCGCAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428996.1
CDS	263..4003
Translation	MQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQEDQLSSLGSADPGVKTEGGGGGGGGGGEQGGAVTNDQGPTVSSSGIFSNTSPHNGVLPQIVDLPSSPTPDSQLSQNEELRGNLKTTSQATGTTATATTGVAD

Target ORF information:

RefSeq Version	XM_008430774.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430774.2

ORF Insert Sequence:

ATGCAGAGCT TCAAAGAGTC CCACTCCCAT GAGTCCCTGC TGTCTCCCAG CAGCGCTGCC 
GAGGCGCTGG ACCTCACACT GGACGAGGAC GCCATCATCA AACCCGTCCA CTCCAGCATC 
CTGGGACAGG AGTACTGTTT TGAGGTGACA ACGGCGTCAG GAACCAAGTG CTTTGCGTGT 
CGCTCAGCCG CAGAGAGAGA CAAATGGATC GAGAACCTGC AGAGAGCTGT CAAGCCCAAC 
AAGGACAACA GCCGGAGGGT GGACAATGTG CTGAAACTGT GGATAATCGA AGCCCGCGAG 
CTCCCGGCTA AGAAGCGCTA CTACTGCGAG CTGTGCCTGG ACGACATGCT GTACGCCCGC 
ACCACCAGCA AGCCCCGCAC CGACACCGTC TTCTGGGGAG AGCACTTTGA GTTCAACAAC 
CTGCCGGCCG TCCGCAACCT TCGCCTTCAT CTGTACAAGG AGACGGACAA GAAGAGACGG 
AAGGAAAAGA GCACATACTT AGGATTGGTC AGCATCCCCA TCTCGAGCAT CACCGGGCGG 
CAGTTTGTGG AGCAATGGTA CCCAGTCATC CAGCCCAGCG TCCTCACAAA GGGAGCCGGC 
GTCGGAGGAG GGAAAATCAT CAACGCCTCG CTTCGCCTCA AATCCCGCTT CCAAACCATG 
AACATCCTGC CCATGGAGCT GTACAAGGAG TTCGCCGAGT ACGTCACCAA CAACTACCGC 
ACCCTGTGCG CCGTGCTGGA GCCCGTGCTG AGCGTGAAGA GTAAAGAGGA AGTGGCCTGT 
GCTTTGGTGC ATATCCTGCA AAGCACAGGG AAGGCGAAGG ACTTCCTGTC CGACATGGCC 
ATGTGTGAGG TAGACAGATT TATCGACCGA GAACACCTTA TATTCAGAGA GAACACTCTG 
GCCACCAAAG CCATAGAAGA GTACCTCAAA CTGATCGGAC ATAAGTACCT CAAGGATGCT 
ATCGGGGAGT TCATAAGGGC CTTGTATGAG TCAGAGGAAA ATTGCGAAGT GGACCCTATG 
AGAATACCAC CCTCTGTGCT ACCAGACCAC CAAGCCAATC TTCGAATGTG CTGCGAACTG 
GCTCTCTGCA AAATCGTTAA TTCCCATTGT GTTTTCCCCC GTGAGCTAAA GGAGGTTTTT 
GCGTCCTGGC GAGTGCGTTG TGCCGAGAGG GGTCGGGAAG ACATCGCTGA CCGCCTCATC 
AGTGGATCGC TCTTCCTGCG CTTCCTGTGT CCCGCCATCA TGTCCCCGTC ACTTTTCAAT 
CTCACCCAGG AGTATCCCGA CGAGCAGACG TCACGCACGC TCACTCTTAT CGCTAAAGTA 
GTGCAAAACC TCGCAAACTT CTCCAAGTTT GGCAGTAAGG AGGAGTACAT GTGTTTCATG 
AATGAGTTTT TAGAGATGGA GTGGGGCTCC ATGCAGCAGT TCCTGTACGA GATCTCCAAC 
CTGGACAGCG TCAGCAACGC AGGCGGCTTT GAGGGATACA TCGATCTGGG CAGGGAGCTG 
TCCATCCTGC ACAGCCTCCT GTGGGAGGTT ATGGCTCAGC TCAGCAAGGA TGCCATCATC 
AAGCTGGGCC CTTTGCCCCG CCTCCTGAAT GACATCAGCA TGGCGTTGCG TAACCCTCAC 
CTCCAGAGGC AGCCGAGCCA CCAGACGGAC CGGGTGCAGG ACAGACAGTC GGACAGGCTG 
CTGTCTCGAC CAAGCTTTAA CCGAGGAATC TCGTCAGAGT TCCAGAATCT CATGATGAGG 
GACTTAAACA GCTCCATAGA CATCACTCGT TTGCCTTCCC CTACTTCCAC CGGAGGGGTC 
ATGCCATCCC GCGCCCAGCT GAGCTTTCAG GACCGCGATC ACCCTCATCG AGCCTCCAAA 
GACATGTTCT ACGTTTCGCG GCCGCCCCTG GCCCGCTCCA GCCCGGCCTA CTGCACGAGC 
AGCTCTGACA TCACGGAACC AGACGCTAAG GATTCCCGAA TGAACAGCGT GTCTAACCTG 
CAGTCGGTGG ACATGCTCAA CTCCTCCCAG GCCTCCATCG CCGGTATGGG CAGCTTCGGT 
GGGCTGAGCA GCGGAGGCGG TGGCGGCCTG GGGTCGGGCC TGAGCAGCCA GCTGCGGGCC 
GGTGGGAGGT TGTCAGCTGG CTCCGGCGGC TCCAGCATGT CAGGCGGCCT CCGGCTGAGC 
CAGCTGAGCC AGATGGGCAC CACCACCGAC TCGCTATCCC AGCAGCAGCA ACACCAGGCC 
GCCGCCATGC GCTACCCGCT TTCCTTCCAG AATCCTCTCT TTCATCTGGC GACGGCTGAC 
GGGCCACAAC AACAGCTCCA TCACCAGCAC AGCCGAGCTC AGCCCCCAGC GCCCCTGCTC 
CTGGCCCCTG ACCCAGAGCC CTCGCACCAG GCCTACATCC CACAGTTCGC CCATGGGGGG 
TTCTCTCGCA GTGAGGATCT GTCCACGCTC AGGACCCGGG ATGGTCACCT GGGCCAGCCC 
AGCATAATCC ACTCGCACAG CTACAGTGAC GACTACAGCA GGGCTGACTA TGGACGCAGG 
CAAATCTCCA TGCATATGCA GGATAACCTC CAACAGCAAC ACGAAATGAT GGGAATGGCC 
TCCCAGACTG GAACGTCCCA CTCCTCCCTG GCCACCACTC CTCCCTCCAC CGTTCAACCT 
ATGCGGCAGA GCTCTGTTGC TCCGCCTCCC AGCCAGCGCG TCAAGTCCCA GACTTCCCAC 
CAGCTATCCG TCAGCGCGGC GGCCGCGCCC GCCGCCTCTG GTAAAAGCCG TCCACAGAGC 
GGGAACCTCC TCCAGTCTCC AGAGTCTGGC TACGGCGGCA GGCAGCACGG GCCCCGGCAG 
CTATCGGTCA AAGACAACAC TGCCCCGGGT CTTCCCCACC AGCAGAGCTC TGTCAGGGAA 
AGCGGGAGTC CACAAGGGAC CACCAGTCAG AGCACTCAGC AGTCACCACA GCAGTCTCAA 
CAGCATCTTT TAAAACCCAC CATGAGTAAA CAGGGCTCCC AGTCACCTAC CACCCTCAAT 
CCCCCGACCC CAGCGAACGA GCGAACAGTG GCCTGGGTCT CCAACATGCC TCACCTGTCG 
GCCGACATCG AAAGTTCACG AATTGACCGA GAGGAGTTCA AGCTGAAGGA GTATTCGAAA 
AGCATGGATG AGTCGCGCAT GGACAGGGTC AAAGAGTACG AGGAGGAAAT CAACTCTTTG 
AAGGAGCGTC TGCTGATGTC TCACAGAAAG CTGGAGGAGT ACGAGAGGAG GCTCCTAGTG 
CAGGAGCAGC AGACCAATAA GATCCTCCTC CAGTACCAAA ACCGTCTGGA GGACAGCGAG 
AGGAGGCTAC GACAACAGCA AGTGGAGAAA GACTCCCAAA TTAAAGGAAT AATTAACAGA 
CTCATGGCTG TGGAGGATGA AATAAGGGGA GGAGCCATTT ACGAGCCAAA AACCAGGATT 
TTCGCTGATC AGGAGGACCA GCTTTCCTCC CTGGGTTCAG CAGACCCCGG TGTGAAAACT 
GAAGGTGGAG GGGGAGGAGG AGGAGGAGGA GGAGAACAAG GAGGAGCGGT TACCAACGAT 
CAAGGCCCCA CAGTCTCTTC CTCTGGCATT TTCTCCAACA CATCGCCCCA CAATGGAGTC 
CTCCCCCAAA TTGTAGACCT TCCCTCCTCA CCCACCCCTG ATTCACAACT TAGTCAGAAT 
GAAGAGCTCC GTGGCAATCT AAAGACAACT TCACAGGCAA CTGGAACCAC AGCAACGGCA 
ACTACGGGAG TCGCAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430775.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428997.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X16, mRNA.

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CloneID	OPo178218
Clone ID Related Accession (Same CDS sequence)	XM_008430775.1
Accession Version	XM_008430775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCTCC CCCGCTTCCG CAGTGCCGAC CAAGACAGGA CCCGTTTGAT GCAGAGCTTC 
AAAGAGTCCC ACTCCCATGA GTCCCTGCTG TCTCCCAGCA GCGCTGCCGA GGCGCTGGAC 
CTCACACTGG ACGAGGACGC CATCATCAAA CCCGTCCACT CCAGCATCCT GGGACAGGAG 
TACTGTTTTG AGGTGACAAC GGCGTCAGGA ACCAAGTGCT TTGCGTGTCG CTCAGCCGCA 
GAGAGAGACA AATGGATCGA GAACCTGCAG AGAGCTGTCA AGCCCAACAA GGACAACAGC 
CGGAGGGTGG ACAATGTGCT GAAACTGTGG ATAATCGAAG CCCGCGAGCT CCCGGCTAAG 
AAGCGCTACT ACTGCGAGCT GTGCCTGGAC GACATGCTGT ACGCCCGCAC CACCAGCAAG 
CCCCGCACCG ACACCGTCTT CTGGGGAGAG CACTTTGAGT TCAACAACCT GCCGGCCGTC 
CGCAACCTTC GCCTTCATCT GTACAAGGAG ACGGACAAGA AGAGACGGAA GGAAAAGAGC 
ACATACTTAG GATTGGTCAG CATCCCCATC TCGAGCATCA CCGGGCGGCA GTTTGTGGAG 
CAATGGTACC CAGTCATCCA GCCCAGCGTC CTCACAAAGG GAGCCGGCGT CGGAGGAGGG 
AAAATCATCA ACGCCTCGCT TCGCCTCAAA TCCCGCTTCC AAACCATGAA CATCCTGCCC 
ATGGAGCTGT ACAAGGAGTT CGCCGAGTAC GTCACCAACA ACTACCGCAC CCTGTGCGCC 
GTGCTGGAGC CCGTGCTGAG CGTGAAGAGT AAAGAGGAAG TGGCCTGTGC TTTGGTGCAT 
ATCCTGCAAA GCACAGGGAA GGCGAAGGAC TTCCTGTCCG ACATGGCCAT GTGTGAGGTA 
GACAGATTTA TCGACCGAGA ACACCTTATA TTCAGAGAGA ACACTCTGGC CACCAAAGCC 
ATAGAAGAGT ACCTCAAACT GATCGGACAT AAGTACCTCA AGGATGCTAT CGGGGAGTTC 
ATAAGGGCCT TGTATGAGTC AGAGGAAAAT TGCGAAGTGG ACCCTATGAG AATACCACCC 
TCTGTGCTAC CAGACCACCA AGCCAATCTT CGAATGTGCT GCGAACTGGC TCTCTGCAAA 
ATCGTTAATT CCCATTGTGT TTTCCCCCGT GAGCTAAAGG AGGTTTTTGC GTCCTGGCGA 
GTGCGTTGTG CCGAGAGGGG TCGGGAAGAC ATCGCTGACC GCCTCATCAG TGGATCGCTC 
TTCCTGCGCT TCCTGTGTCC CGCCATCATG TCCCCGTCAC TTTTCAATCT CACCCAGGAG 
TATCCCGACG AGCAGACGTC ACGCACGCTC ACTCTTATCG CTAAAGTAGT GCAAAACCTC 
GCAAACTTCT CCAAGTTTGG CAGTAAGGAG GAGTACATGT GTTTCATGAA TGAGTTTTTA 
GAGATGGAGT GGGGCTCCAT GCAGCAGTTC CTGTACGAGA TCTCCAACCT GGACAGCGTC 
AGCAACGCAG GCGGCTTTGA GGGATACATC GATCTGGGCA GGGAGCTGTC CATCCTGCAC 
AGCCTCCTGT GGGAGGTTAT GGCTCAGCTC AGCAAGGATG CCATCATCAA GCTGGGCCCT 
TTGCCCCGCC TCCTGAATGA CATCAGCATG GCGTTGCGTA ACCCTCACCT CCAGAGGCAG 
CCGAGCCACC AGACGGACCG GGTGCAGGAC AGACAGTCGG ACAGGCTGCT GTCTCGACCA 
AGCTTTAACC GAGGAATCTC GTCAGAGTTC CAGAATCTCA TGATGAGGGA CTTAAACAGC 
TCCATAGACA TCACTCGTTT GCCTTCCCCT ACTTCCACCG GAGGGGTCAT GCCATCCCGC 
GCCCAGCTGA GCTTTCAGGA CCGCGATCAC CCTCATCGAG CCTCCAAAGA CATGTTCTAC 
GTTTCGCGGC CGCCCCTGGC CCGCTCCAGC CCGGCCTACT GCACGAGCAG CTCTGACATC 
ACGGAACCAG ACGCTAAGGA TTCCCGAATG AACAGCGTGT CTAACCTGCA GTCGGTGGAC 
ATGCTCAACT CCTCCCAGGC CTCCATCGCC GGTATGGGCA GCTTCGGTGG GCTGAGCAGC 
GGAGGCGGTG GCGGCCTGGG GTCGGGCCTG AGCAGCCAGC TGCGGGCCGG TGGGAGGTTG 
TCAGCTGGCT CCGGCGGCTC CAGCATGTCA GGCGGCCTCC GGCTGAGCCA GCTGAGCCAG 
ATGGGCACCA CCACCGACTC GCTATCCCAG CAGCAGCAAC ACCAGGCCGC CGCCATGCGC 
TACCCGCTTT CCTTCCAGAA TCCTCTCTTT CATCTGGCGA CGGCTGACGG GCCACAACAA 
CAGCTCCATC ACCAGCACAG CCGAGCTCAG CCCCCAGCGC CCCTGCTCCT GGCCCCTGAC 
CCAGAGCCCT CGCACCAGGC CTACATCCCA CAGTTCGCCC ATGGGGGGTT CTCTCGCAGT 
GAGGATCTGT CCACGCTCAG GACCCGGGAT GGTCACCTGG GCCAGCCCAG CATAATCCAC 
TCGCACAGCT ACAGTGACGA CTACAGCAGG GCTGACTATG GACGCAGGCA AATCTCCATG 
CATATGCAGG ATAACCTCCA ACAGCAACAC GAAATGATGG GAATGGCCTC CCAGACTGGA 
ACGTCCCACT CCTCCCTGGC CACCACTCCT CCCTCCACCG TTCAACCTAT GCGGCAGAGC 
TCTGTTGCTC CGCCTCCCAG CCAGCGCGTC AAGTCCCAGA CTTCCCACCA GCTATCCGTC 
AGCGCGGCGG CCGCGCCCGC CGCCTCTGGT AAAAGCCGTC CACAGAGCGG GAACCTCCTC 
CAGTCTCCAG AGTCTGGCTA CGGCGGCAGG CAGCACGGGC CCCGGCAGCT ATCGGTCAAA 
GACAACACTG CCCCGGGTCT TCCCCACCAG CAGAGCTCTG TCAGGGAAAG CGGGAGTCCA 
CAAGGGACCA CCAGTCAGAG CACTCAGCAG TCACCACAGC AGTCTCAACA GCATCTTTTA 
AAACCCACCA TGAGTAAACA GGGCTCCCAG TCACCTACCA CCCTCAATCC CCCGACCCCA 
GCGAACGAGC GAACAGTGGC CTGGGTCTCC AACATGCCTC ACCTGTCGGC CGACATCGAA 
AGTTCACGAA TTGACCGAGA GGAGTTCAAG CTGAAGGAGT ATTCGAAAAG CATGGATGAG 
TCGCGCATGG ACAGGGTCAA AGAGTACGAG GAGGAAATCA ACTCTTTGAA GGAGCGTCTG 
CTGATGTCTC ACAGAAAGCT GGAGGAGTAC GAGAGGAGGC TCCTAGTGCA GGAGCAGCAG 
ACCAATAAGA TCCTCCTCCA GTACCAAAAC CGTCTGGAGG ACAGCGAGAG GAGGCTACGA 
CAACAGCAAG TGGAGAAAGA CTCCCAAATT AAAGGAATAA TTAACAGACT CATGGCTGTG 
GAGGATGAAA TAAGGGGAGG AGCCATTTAC GAGCCAAAAA CCAGGATTTT CGCTGATCAG 
GAGGACCAGC TTTCCTCCCT GGGTTCAGCA GACCCCGGTG TGAAAACTGA AGGTGGAGGG 
GGAGGAGGAG GAGGAGGAGG AGAACAAGGA GGAGCGGTTA CCAACGATCA AGGCCCCACA 
GTCTCTTCCT CTGGCATTTT CTCCAACACA TCGCCCCACA ATGGAGTCCT CCCCCAAATT 
GTAGACCTTC CCTCCTCACC CACCCCTGAT TCACAACTTA GTCAGAATGA AGAGCTCCGT 
GGCAATCTAA AGACAACTTC ACAGGCAACT GGAACCACAG CAACGGCAAC TACGGGAGTC 
GCAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428997.1
CDS	469..4257
Translation	MILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQEDQLSSLGSADPGVKTEGGGGGGGGGGEQGGAVTNDQGPTVSSSGIFSNTSPHNGVLPQIVDLPSSPTPDSQLSQNEELRGNLKTTSQATGTTATATTGVAD

Target ORF information:

RefSeq Version	XM_008430775.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430775.1

ORF Insert Sequence:

ATGATCCTCC CCCGCTTCCG CAGTGCCGAC CAAGACAGGA CCCGTTTGAT GCAGAGCTTC 
AAAGAGTCCC ACTCCCATGA GTCCCTGCTG TCTCCCAGCA GCGCTGCCGA GGCGCTGGAC 
CTCACACTGG ACGAGGACGC CATCATCAAA CCCGTCCACT CCAGCATCCT GGGACAGGAG 
TACTGTTTTG AGGTGACAAC GGCGTCAGGA ACCAAGTGCT TTGCGTGTCG CTCAGCCGCA 
GAGAGAGACA AATGGATCGA GAACCTGCAG AGAGCTGTCA AGCCCAACAA GGACAACAGC 
CGGAGGGTGG ACAATGTGCT GAAACTGTGG ATAATCGAAG CCCGCGAGCT CCCGGCTAAG 
AAGCGCTACT ACTGCGAGCT GTGCCTGGAC GACATGCTGT ACGCCCGCAC CACCAGCAAG 
CCCCGCACCG ACACCGTCTT CTGGGGAGAG CACTTTGAGT TCAACAACCT GCCGGCCGTC 
CGCAACCTTC GCCTTCATCT GTACAAGGAG ACGGACAAGA AGAGACGGAA GGAAAAGAGC 
ACATACTTAG GATTGGTCAG CATCCCCATC TCGAGCATCA CCGGGCGGCA GTTTGTGGAG 
CAATGGTACC CAGTCATCCA GCCCAGCGTC CTCACAAAGG GAGCCGGCGT CGGAGGAGGG 
AAAATCATCA ACGCCTCGCT TCGCCTCAAA TCCCGCTTCC AAACCATGAA CATCCTGCCC 
ATGGAGCTGT ACAAGGAGTT CGCCGAGTAC GTCACCAACA ACTACCGCAC CCTGTGCGCC 
GTGCTGGAGC CCGTGCTGAG CGTGAAGAGT AAAGAGGAAG TGGCCTGTGC TTTGGTGCAT 
ATCCTGCAAA GCACAGGGAA GGCGAAGGAC TTCCTGTCCG ACATGGCCAT GTGTGAGGTA 
GACAGATTTA TCGACCGAGA ACACCTTATA TTCAGAGAGA ACACTCTGGC CACCAAAGCC 
ATAGAAGAGT ACCTCAAACT GATCGGACAT AAGTACCTCA AGGATGCTAT CGGGGAGTTC 
ATAAGGGCCT TGTATGAGTC AGAGGAAAAT TGCGAAGTGG ACCCTATGAG AATACCACCC 
TCTGTGCTAC CAGACCACCA AGCCAATCTT CGAATGTGCT GCGAACTGGC TCTCTGCAAA 
ATCGTTAATT CCCATTGTGT TTTCCCCCGT GAGCTAAAGG AGGTTTTTGC GTCCTGGCGA 
GTGCGTTGTG CCGAGAGGGG TCGGGAAGAC ATCGCTGACC GCCTCATCAG TGGATCGCTC 
TTCCTGCGCT TCCTGTGTCC CGCCATCATG TCCCCGTCAC TTTTCAATCT CACCCAGGAG 
TATCCCGACG AGCAGACGTC ACGCACGCTC ACTCTTATCG CTAAAGTAGT GCAAAACCTC 
GCAAACTTCT CCAAGTTTGG CAGTAAGGAG GAGTACATGT GTTTCATGAA TGAGTTTTTA 
GAGATGGAGT GGGGCTCCAT GCAGCAGTTC CTGTACGAGA TCTCCAACCT GGACAGCGTC 
AGCAACGCAG GCGGCTTTGA GGGATACATC GATCTGGGCA GGGAGCTGTC CATCCTGCAC 
AGCCTCCTGT GGGAGGTTAT GGCTCAGCTC AGCAAGGATG CCATCATCAA GCTGGGCCCT 
TTGCCCCGCC TCCTGAATGA CATCAGCATG GCGTTGCGTA ACCCTCACCT CCAGAGGCAG 
CCGAGCCACC AGACGGACCG GGTGCAGGAC AGACAGTCGG ACAGGCTGCT GTCTCGACCA 
AGCTTTAACC GAGGAATCTC GTCAGAGTTC CAGAATCTCA TGATGAGGGA CTTAAACAGC 
TCCATAGACA TCACTCGTTT GCCTTCCCCT ACTTCCACCG GAGGGGTCAT GCCATCCCGC 
GCCCAGCTGA GCTTTCAGGA CCGCGATCAC CCTCATCGAG CCTCCAAAGA CATGTTCTAC 
GTTTCGCGGC CGCCCCTGGC CCGCTCCAGC CCGGCCTACT GCACGAGCAG CTCTGACATC 
ACGGAACCAG ACGCTAAGGA TTCCCGAATG AACAGCGTGT CTAACCTGCA GTCGGTGGAC 
ATGCTCAACT CCTCCCAGGC CTCCATCGCC GGTATGGGCA GCTTCGGTGG GCTGAGCAGC 
GGAGGCGGTG GCGGCCTGGG GTCGGGCCTG AGCAGCCAGC TGCGGGCCGG TGGGAGGTTG 
TCAGCTGGCT CCGGCGGCTC CAGCATGTCA GGCGGCCTCC GGCTGAGCCA GCTGAGCCAG 
ATGGGCACCA CCACCGACTC GCTATCCCAG CAGCAGCAAC ACCAGGCCGC CGCCATGCGC 
TACCCGCTTT CCTTCCAGAA TCCTCTCTTT CATCTGGCGA CGGCTGACGG GCCACAACAA 
CAGCTCCATC ACCAGCACAG CCGAGCTCAG CCCCCAGCGC CCCTGCTCCT GGCCCCTGAC 
CCAGAGCCCT CGCACCAGGC CTACATCCCA CAGTTCGCCC ATGGGGGGTT CTCTCGCAGT 
GAGGATCTGT CCACGCTCAG GACCCGGGAT GGTCACCTGG GCCAGCCCAG CATAATCCAC 
TCGCACAGCT ACAGTGACGA CTACAGCAGG GCTGACTATG GACGCAGGCA AATCTCCATG 
CATATGCAGG ATAACCTCCA ACAGCAACAC GAAATGATGG GAATGGCCTC CCAGACTGGA 
ACGTCCCACT CCTCCCTGGC CACCACTCCT CCCTCCACCG TTCAACCTAT GCGGCAGAGC 
TCTGTTGCTC CGCCTCCCAG CCAGCGCGTC AAGTCCCAGA CTTCCCACCA GCTATCCGTC 
AGCGCGGCGG CCGCGCCCGC CGCCTCTGGT AAAAGCCGTC CACAGAGCGG GAACCTCCTC 
CAGTCTCCAG AGTCTGGCTA CGGCGGCAGG CAGCACGGGC CCCGGCAGCT ATCGGTCAAA 
GACAACACTG CCCCGGGTCT TCCCCACCAG CAGAGCTCTG TCAGGGAAAG CGGGAGTCCA 
CAAGGGACCA CCAGTCAGAG CACTCAGCAG TCACCACAGC AGTCTCAACA GCATCTTTTA 
AAACCCACCA TGAGTAAACA GGGCTCCCAG TCACCTACCA CCCTCAATCC CCCGACCCCA 
GCGAACGAGC GAACAGTGGC CTGGGTCTCC AACATGCCTC ACCTGTCGGC CGACATCGAA 
AGTTCACGAA TTGACCGAGA GGAGTTCAAG CTGAAGGAGT ATTCGAAAAG CATGGATGAG 
TCGCGCATGG ACAGGGTCAA AGAGTACGAG GAGGAAATCA ACTCTTTGAA GGAGCGTCTG 
CTGATGTCTC ACAGAAAGCT GGAGGAGTAC GAGAGGAGGC TCCTAGTGCA GGAGCAGCAG 
ACCAATAAGA TCCTCCTCCA GTACCAAAAC CGTCTGGAGG ACAGCGAGAG GAGGCTACGA 
CAACAGCAAG TGGAGAAAGA CTCCCAAATT AAAGGAATAA TTAACAGACT CATGGCTGTG 
GAGGATGAAA TAAGGGGAGG AGCCATTTAC GAGCCAAAAA CCAGGATTTT CGCTGATCAG 
GAGGACCAGC TTTCCTCCCT GGGTTCAGCA GACCCCGGTG TGAAAACTGA AGGTGGAGGG 
GGAGGAGGAG GAGGAGGAGG AGAACAAGGA GGAGCGGTTA CCAACGATCA AGGCCCCACA 
GTCTCTTCCT CTGGCATTTT CTCCAACACA TCGCCCCACA ATGGAGTCCT CCCCCAAATT 
GTAGACCTTC CCTCCTCACC CACCCCTGAT TCACAACTTA GTCAGAATGA AGAGCTCCGT 
GGCAATCTAA AGACAACTTC ACAGGCAACT GGAACCACAG CAACGGCAAC TACGGGAGTC 
GCAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430762.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428984.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo178208
Clone ID Related Accession (Same CDS sequence)	XM_008430762.2
Accession Version	XM_008430762.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008430762.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG AGGACCAGCT TTCCTCCCTG GGTTCAGCAG ACCCCGGTGT GAAAACTGAA 
GGTGGAGGGG GAGGAGGAGG AGGAGGAGGA GAACAAGGAG GAGCGGTTAC CAACGATCAA 
GGCCCCACAG TCTCTTCCTC TGGCATTTTC TCCAACACAT CGCCCCACAA TGGAGTCCTC 
CCCCAAATTG TAGACCTTCC CTCCTCACCC ACCCCTGATT CACAACTTAG TCAGAATGAA 
GAGCTCCGTG GCAATCTAAA GACAACTTCA CAGGCAACTG GAACCACAGC AACGGCAACT 
ACGGGAGTCG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428984.1
CDS	364..4881
Translation	MSYVPFQDARCAVPPSYHHHHHHPHRSQAQPFHAPSYLRPSHERPSYDRPSFDRPSFERPSFDRPSFERPSFERPSFERPSFERPSFERPSFDRPSFDRPAYERPSYHRPLHERPIYNPPLHDRQPHDRWNPRRCVSSGHQYNMLDQEEVHPLLMRERRSESHRTKLLRRTVSVPVEGRHHPEMDHRARRKSIATGKQPSMEVPPTAPLQPFRQSSFLSKRLKGSIKRAKSQPKLDRTSSFRQMILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQEDQLSSLGSADPGVKTEGGGGGGGGGGEQGGAVTNDQGPTVSSSGIFSNTSPHNGVLPQIVDLPSSPTPDSQLSQNEELRGNLKTTSQATGTTATATTGVAD

Target ORF information:

RefSeq Version	XM_008430762.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430762.2

ORF Insert Sequence:

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG AGGACCAGCT TTCCTCCCTG GGTTCAGCAG ACCCCGGTGT GAAAACTGAA 
GGTGGAGGGG GAGGAGGAGG AGGAGGAGGA GAACAAGGAG GAGCGGTTAC CAACGATCAA 
GGCCCCACAG TCTCTTCCTC TGGCATTTTC TCCAACACAT CGCCCCACAA TGGAGTCCTC 
CCCCAAATTG TAGACCTTCC CTCCTCACCC ACCCCTGATT CACAACTTAG TCAGAATGAA 
GAGCTCCGTG GCAATCTAAA GACAACTTCA CAGGCAACTG GAACCACAGC AACGGCAACT 
ACGGGAGTCG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430763.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428985.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo178209
Clone ID Related Accession (Same CDS sequence)	XM_008430763.2
Accession Version	XM_008430763.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008430763.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG AGGACCAGCT TTCCTCCCTG GGTTCAGCAG ACCCCGGTGT GAAAACTGAA 
GGTGGAGGGG GAGGAGGAGG AGGAGGAGGA GAACAAGGAG GAGCGGTTAC CAACGATCAA 
GGCCCCACAG TCTCTTCCTC TGGCATTTTC TCCAACACAT CGCCCCACAA TGGAGTCCTC 
CCCCAAATTG TAGACCTTCC CTCCTCACCC ACCCCTGATT CACAACTTAG TCAGAATGAA 
GAGCTCCGTG GCAATCTAAA GACAACTTCA CAGGCAACTG GAACCACAGC AACGGCAACT 
ACGGGAGTCG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428985.1
CDS	364..4821
Translation	MSYVPFQDARCAVPPSYHHHHHHPHRSQAQPFHAPSYLRPSHERPSYDRPSFDRPSFERPSFDRPSFERPSFERPSFERPSFERPSFERPSFDRPSFDRPAYERPSYHRPLHERPIYNPPLHDRQPHDRWNPRRCVSSGHQYNMLDQEEVHPLLMRERRSESHRTKLLRRTVSVPVEGRHHPEMDHRARRKSIATGKQPSMEVPPTAPLQPFRQSSFLSKRLKGSIKRAKSQPKLDRTSSFRQMILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQEDQLSSLGSADPGVKTEGGGGGGGGGGEQGGAVTNDQGPTVSSSGIFSNTSPHNGVLPQIVDLPSSPTPDSQLSQNEELRGNLKTTSQATGTTATATTGVAD

Target ORF information:

RefSeq Version	XM_008430763.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430763.2

ORF Insert Sequence:

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG AGGACCAGCT TTCCTCCCTG GGTTCAGCAG ACCCCGGTGT GAAAACTGAA 
GGTGGAGGGG GAGGAGGAGG AGGAGGAGGA GAACAAGGAG GAGCGGTTAC CAACGATCAA 
GGCCCCACAG TCTCTTCCTC TGGCATTTTC TCCAACACAT CGCCCCACAA TGGAGTCCTC 
CCCCAAATTG TAGACCTTCC CTCCTCACCC ACCCCTGATT CACAACTTAG TCAGAATGAA 
GAGCTCCGTG GCAATCTAAA GACAACTTCA CAGGCAACTG GAACCACAGC AACGGCAACT 
ACGGGAGTCG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430765.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428987.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X5, mRNA.

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CloneID	OPo178210
Clone ID Related Accession (Same CDS sequence)	XM_008430765.2
Accession Version	XM_008430765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008430765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG GGAGGCGTCA GTCCATCCTA GTGCAGCCCC GACTTGCGCC TGTCCCACCC 
CGAGTACCCA GCCACTCTCA AAGCTTCATG GAGGACAATG TGGAACCTAA TTGGCTGATG 
CACCATCGGC AGTCTTCGGG GTGGCATGGC CAAATGTCCA GAGCCCTCTC TCGTGACGCG 
ATTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428987.1
CDS	364..4752
Translation	MSYVPFQDARCAVPPSYHHHHHHPHRSQAQPFHAPSYLRPSHERPSYDRPSFDRPSFERPSFDRPSFERPSFERPSFERPSFERPSFERPSFDRPSFDRPAYERPSYHRPLHERPIYNPPLHDRQPHDRWNPRRCVSSGHQYNMLDQEEVHPLLMRERRSESHRTKLLRRTVSVPVEGRHHPEMDHRARRKSIATGKQPSMEVPPTAPLQPFRQSSFLSKRLKGSIKRAKSQPKLDRTSSFRQMILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQGRRQSILVQPRLAPVPPRVPSHSQSFMEDNVEPNWLMHHRQSSGWHGQMSRALSRDAIG

Target ORF information:

RefSeq Version	XM_008430765.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430765.2

ORF Insert Sequence:

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG GGAGGCGTCA GTCCATCCTA GTGCAGCCCC GACTTGCGCC TGTCCCACCC 
CGAGTACCCA GCCACTCTCA AAGCTTCATG GAGGACAATG TGGAACCTAA TTGGCTGATG 
CACCATCGGC AGTCTTCGGG GTGGCATGGC CAAATGTCCA GAGCCCTCTC TCGTGACGCG 
ATTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430766.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428988.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X6, mRNA.

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CloneID	OPo178211
Clone ID Related Accession (Same CDS sequence)	XM_008430766.2
Accession Version	XM_008430766.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008430766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG GGAGGCGTCA GTCCATCCTA GTGCAGCCCC GACTTGCGCC TGTCCCACCC 
CGAGTACCCA GGAGGACCAG CTTTCCTCCC TGGGTTCAGC AGACCCCGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428988.1
CDS	364..4677
Translation	MSYVPFQDARCAVPPSYHHHHHHPHRSQAQPFHAPSYLRPSHERPSYDRPSFDRPSFERPSFDRPSFERPSFERPSFERPSFERPSFERPSFDRPSFDRPAYERPSYHRPLHERPIYNPPLHDRQPHDRWNPRRCVSSGHQYNMLDQEEVHPLLMRERRSESHRTKLLRRTVSVPVEGRHHPEMDHRARRKSIATGKQPSMEVPPTAPLQPFRQSSFLSKRLKGSIKRAKSQPKLDRTSSFRQMILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQGRRQSILVQPRLAPVPPRVPRRTSFPPWVQQTPV

Target ORF information:

RefSeq Version	XM_008430766.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430766.2

ORF Insert Sequence:

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG GGAGGCGTCA GTCCATCCTA GTGCAGCCCC GACTTGCGCC TGTCCCACCC 
CGAGTACCCA GGAGGACCAG CTTTCCTCCC TGGGTTCAGC AGACCCCGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430767.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428989.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPo178212
Clone ID Related Accession (Same CDS sequence)	XM_008430767.2
Accession Version	XM_008430767.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008430767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGC CACTCTCAAA GCTTCATGGA GGACAATGTG GAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428989.1
CDS	364..4611
Translation	MSYVPFQDARCAVPPSYHHHHHHPHRSQAQPFHAPSYLRPSHERPSYDRPSFDRPSFERPSFDRPSFERPSFERPSFERPSFERPSFERPSFDRPSFDRPAYERPSYHRPLHERPIYNPPLHDRQPHDRWNPRRCVSSGHQYNMLDQEEVHPLLMRERRSESHRTKLLRRTVSVPVEGRHHPEMDHRARRKSIATGKQPSMEVPPTAPLQPFRQSSFLSKRLKGSIKRAKSQPKLDRTSSFRQMILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQPLSKLHGGQCGT

Target ORF information:

RefSeq Version	XM_008430767.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430767.2

ORF Insert Sequence:

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGC CACTCTCAAA GCTTCATGGA GGACAATGTG GAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430768.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428990.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OPo178213
Clone ID Related Accession (Same CDS sequence)	XM_008430768.2
Accession Version	XM_008430768.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008430768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG AGGACCAGCT TTCCTCCCTG GGTTCAGCAG ACCCCGGTGT GAAAACTGAA 
GGTGGAGGGG GAGGAGGAGG AGGAGGAGGA GAACAAGGAG GAGCGGTTAC CAACGATCAA 
GGCCCCACAG TCTCTTCCTC TGGCATTTTC TCCAACACAT CGCCCCACAA TGGAGTCCTC 
CCCCAAATTG TAGACCTTCC CTCCTCACCC ACCCCTGATT CACAACTTAG TCAGAATGAA 
GAGCTCCGTG GCAATCTAAA GACAACTTCA CAGGCAACTG GAACCACAGC AACGGCAACT 
ACGGGAGTCG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428990.1
CDS	364..4581
Translation	MSYVPFQDARCAVPPSYHHHHHHPHRSQAQPFHAPSYLRPSHERPSYDRPSFDRPSFERPSFDRPSFERPSFERPSFERPSFERPSFERPSFDRPSFDRPAYERPSYHRPLHERPIYNPPLHDRQPHDRWNPRRCVSSGHQYNMLDQEEVHPLLMRERRSESHRTKLLRRTVSVPVEGRHHPEMDHRARRKSIATGKQPSMEVPPTAPLQPFRQSSFLSKRLKGSIKRAKSQPKLDRTSSFRQMILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRLMAVEDEIRGGAIYEPKTRIFADQEDQLSSLGSADPGVKTEGGGGGGGGGGEQGGAVTNDQGPTVSSSGIFSNTSPHNGVLPQIVDLPSSPTPDSQLSQNEELRGNLKTTSQATGTTATATTGVAD

Target ORF information:

RefSeq Version	XM_008430768.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430768.2

ORF Insert Sequence:

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGACTC 
ATGGCTGTGG AGGATGAAAT AAGGGGAGGA GCCATTTACG AGCCAAAAAC CAGGATTTTC 
GCTGATCAGG AGGACCAGCT TTCCTCCCTG GGTTCAGCAG ACCCCGGTGT GAAAACTGAA 
GGTGGAGGGG GAGGAGGAGG AGGAGGAGGA GAACAAGGAG GAGCGGTTAC CAACGATCAA 
GGCCCCACAG TCTCTTCCTC TGGCATTTTC TCCAACACAT CGCCCCACAA TGGAGTCCTC 
CCCCAAATTG TAGACCTTCC CTCCTCACCC ACCCCTGATT CACAACTTAG TCAGAATGAA 
GAGCTCCGTG GCAATCTAAA GACAACTTCA CAGGCAACTG GAACCACAGC AACGGCAACT 
ACGGGAGTCG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103477581 ( XM_008430770.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008428992.1 Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X10, mRNA.

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CloneID	OPo178214
Clone ID Related Accession (Same CDS sequence)	XM_008430770.2
Accession Version	XM_008430770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ras/Rap GTPase-activating protein SynGAP isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008430770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGGAAA 
GAAGACTCGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008428992.1
CDS	364..4518
Translation	MSYVPFQDARCAVPPSYHHHHHHPHRSQAQPFHAPSYLRPSHERPSYDRPSFDRPSFERPSFDRPSFERPSFERPSFERPSFERPSFERPSFDRPSFDRPAYERPSYHRPLHERPIYNPPLHDRQPHDRWNPRRCVSSGHQYNMLDQEEVHPLLMRERRSESHRTKLLRRTVSVPVEGRHHPEMDHRARRKSIATGKQPSMEVPPTAPLQPFRQSSFLSKRLKGSIKRAKSQPKLDRTSSFRQMILPRFRSADQDRTRLMQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQEYCFEVTTASGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPAKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRRKEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLTKGAGVGGGKIINASLRLKSRFQTMNILPMELYKEFAEYVTNNYRTLCAVLEPVLSVKSKEEVACALVHILQSTGKAKDFLSDMAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCEVDPMRIPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGREDIADRLISGSLFLRFLCPAIMSPSLFNLTQEYPDEQTSRTLTLIAKVVQNLANFSKFGSKEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEVMAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQTDRVQDRQSDRLLSRPSFNRGISSEFQNLMMRDLNSSIDITRLPSPTSTGGVMPSRAQLSFQDRDHPHRASKDMFYVSRPPLARSSPAYCTSSSDITEPDAKDSRMNSVSNLQSVDMLNSSQASIAGMGSFGGLSSGGGGGLGSGLSSQLRAGGRLSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQHQAAAMRYPLSFQNPLFHLATADGPQQQLHHQHSRAQPPAPLLLAPDPEPSHQAYIPQFAHGGFSRSEDLSTLRTRDGHLGQPSIIHSHSYSDDYSRADYGRRQISMHMQDNLQQQHEMMGMASQTGTSHSSLATTPPSTVQPMRQSSVAPPPSQRVKSQTSHQLSVSAAAAPAASGKSRPQSGNLLQSPESGYGGRQHGPRQLSVKDNTAPGLPHQQSSVRESGSPQGTTSQSTQQSPQQSQQHLLKPTMSKQGSQSPTTLNPPTPANERTVAWVSNMPHLSADIESSRIDREEFKLKEYSKSMDESRMDRVKEYEEEINSLKERLLMSHRKLEEYERRLLVQEQQTNKILLQYQNRLEDSERRLRQQQVEKDSQIKGIINRKEDSE

Target ORF information:

RefSeq Version	XM_008430770.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata ras/Rap GTPase-activating protein SynGAP (LOC103477581), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008430770.2

ORF Insert Sequence:

ATGTCCTATG TTCCCTTCCA AGATGCAAGG TGTGCCGTCC CCCCCTCCTA TCATCATCAT 
CATCACCACC CTCATCGCTC CCAAGCCCAG CCCTTCCACG CTCCTTCCTA CCTCCGTCCC 
TCACACGAAC GTCCCTCCTA CGATCGTCCC TCCTTCGATC GTCCCTCCTT TGAGCGTCCC 
TCCTTCGATC GTCCCTCCTT TGAGCGTCCC TCCTTTGAAC GTCCTTCCTT TGAGCGACCT 
TCCTTCGAGC GTCCTTCCTT TGAACGTCCC TCCTTCGATC GCCCATCCTT CGACCGTCCT 
GCCTACGAGC GTCCCTCCTA CCACCGCCCA TTGCACGAGC GCCCCATCTA CAACCCCCCC 
TTGCACGACC GTCAACCCCA CGACCGCTGG AACCCTCGTC GTTGTGTGAG CTCCGGACAT 
CAGTACAACA TGTTGGACCA GGAAGAGGTA CACCCTTTGC TGATGCGCGA GAGGCGCTCA 
GAGTCCCACA GGACCAAGCT ACTGCGTCGG ACAGTCAGCG TTCCTGTTGA GGGAAGGCAT 
CACCCCGAGA TGGATCACCG TGCCCGGAGG AAGAGCATAG CCACTGGGAA GCAACCCAGC 
ATGGAGGTCC CTCCCACTGC CCCCCTTCAA CCCTTCCGAC AATCCAGTTT CCTCAGTAAG 
AGGTTGAAGG GCTCCATCAA GAGGGCTAAG AGTCAGCCGA AACTGGACCG AACGAGCAGT 
TTCCGCCAAA TGATCCTCCC CCGCTTCCGC AGTGCCGACC AAGACAGGAC CCGTTTGATG 
CAGAGCTTCA AAGAGTCCCA CTCCCATGAG TCCCTGCTGT CTCCCAGCAG CGCTGCCGAG 
GCGCTGGACC TCACACTGGA CGAGGACGCC ATCATCAAAC CCGTCCACTC CAGCATCCTG 
GGACAGGAGT ACTGTTTTGA GGTGACAACG GCGTCAGGAA CCAAGTGCTT TGCGTGTCGC 
TCAGCCGCAG AGAGAGACAA ATGGATCGAG AACCTGCAGA GAGCTGTCAA GCCCAACAAG 
GACAACAGCC GGAGGGTGGA CAATGTGCTG AAACTGTGGA TAATCGAAGC CCGCGAGCTC 
CCGGCTAAGA AGCGCTACTA CTGCGAGCTG TGCCTGGACG ACATGCTGTA CGCCCGCACC 
ACCAGCAAGC CCCGCACCGA CACCGTCTTC TGGGGAGAGC ACTTTGAGTT CAACAACCTG 
CCGGCCGTCC GCAACCTTCG CCTTCATCTG TACAAGGAGA CGGACAAGAA GAGACGGAAG 
GAAAAGAGCA CATACTTAGG ATTGGTCAGC ATCCCCATCT CGAGCATCAC CGGGCGGCAG 
TTTGTGGAGC AATGGTACCC AGTCATCCAG CCCAGCGTCC TCACAAAGGG AGCCGGCGTC 
GGAGGAGGGA AAATCATCAA CGCCTCGCTT CGCCTCAAAT CCCGCTTCCA AACCATGAAC 
ATCCTGCCCA TGGAGCTGTA CAAGGAGTTC GCCGAGTACG TCACCAACAA CTACCGCACC 
CTGTGCGCCG TGCTGGAGCC CGTGCTGAGC GTGAAGAGTA AAGAGGAAGT GGCCTGTGCT 
TTGGTGCATA TCCTGCAAAG CACAGGGAAG GCGAAGGACT TCCTGTCCGA CATGGCCATG 
TGTGAGGTAG ACAGATTTAT CGACCGAGAA CACCTTATAT TCAGAGAGAA CACTCTGGCC 
ACCAAAGCCA TAGAAGAGTA CCTCAAACTG ATCGGACATA AGTACCTCAA GGATGCTATC 
GGGGAGTTCA TAAGGGCCTT GTATGAGTCA GAGGAAAATT GCGAAGTGGA CCCTATGAGA 
ATACCACCCT CTGTGCTACC AGACCACCAA GCCAATCTTC GAATGTGCTG CGAACTGGCT 
CTCTGCAAAA TCGTTAATTC CCATTGTGTT TTCCCCCGTG AGCTAAAGGA GGTTTTTGCG 
TCCTGGCGAG TGCGTTGTGC CGAGAGGGGT CGGGAAGACA TCGCTGACCG CCTCATCAGT 
GGATCGCTCT TCCTGCGCTT CCTGTGTCCC GCCATCATGT CCCCGTCACT TTTCAATCTC 
ACCCAGGAGT ATCCCGACGA GCAGACGTCA CGCACGCTCA CTCTTATCGC TAAAGTAGTG 
CAAAACCTCG CAAACTTCTC CAAGTTTGGC AGTAAGGAGG AGTACATGTG TTTCATGAAT 
GAGTTTTTAG AGATGGAGTG GGGCTCCATG CAGCAGTTCC TGTACGAGAT CTCCAACCTG 
GACAGCGTCA GCAACGCAGG CGGCTTTGAG GGATACATCG ATCTGGGCAG GGAGCTGTCC 
ATCCTGCACA GCCTCCTGTG GGAGGTTATG GCTCAGCTCA GCAAGGATGC CATCATCAAG 
CTGGGCCCTT TGCCCCGCCT CCTGAATGAC ATCAGCATGG CGTTGCGTAA CCCTCACCTC 
CAGAGGCAGC CGAGCCACCA GACGGACCGG GTGCAGGACA GACAGTCGGA CAGGCTGCTG 
TCTCGACCAA GCTTTAACCG AGGAATCTCG TCAGAGTTCC AGAATCTCAT GATGAGGGAC 
TTAAACAGCT CCATAGACAT CACTCGTTTG CCTTCCCCTA CTTCCACCGG AGGGGTCATG 
CCATCCCGCG CCCAGCTGAG CTTTCAGGAC CGCGATCACC CTCATCGAGC CTCCAAAGAC 
ATGTTCTACG TTTCGCGGCC GCCCCTGGCC CGCTCCAGCC CGGCCTACTG CACGAGCAGC 
TCTGACATCA CGGAACCAGA CGCTAAGGAT TCCCGAATGA ACAGCGTGTC TAACCTGCAG 
TCGGTGGACA TGCTCAACTC CTCCCAGGCC TCCATCGCCG GTATGGGCAG CTTCGGTGGG 
CTGAGCAGCG GAGGCGGTGG CGGCCTGGGG TCGGGCCTGA GCAGCCAGCT GCGGGCCGGT 
GGGAGGTTGT CAGCTGGCTC CGGCGGCTCC AGCATGTCAG GCGGCCTCCG GCTGAGCCAG 
CTGAGCCAGA TGGGCACCAC CACCGACTCG CTATCCCAGC AGCAGCAACA CCAGGCCGCC 
GCCATGCGCT ACCCGCTTTC CTTCCAGAAT CCTCTCTTTC ATCTGGCGAC GGCTGACGGG 
CCACAACAAC AGCTCCATCA CCAGCACAGC CGAGCTCAGC CCCCAGCGCC CCTGCTCCTG 
GCCCCTGACC CAGAGCCCTC GCACCAGGCC TACATCCCAC AGTTCGCCCA TGGGGGGTTC 
TCTCGCAGTG AGGATCTGTC CACGCTCAGG ACCCGGGATG GTCACCTGGG CCAGCCCAGC 
ATAATCCACT CGCACAGCTA CAGTGACGAC TACAGCAGGG CTGACTATGG ACGCAGGCAA 
ATCTCCATGC ATATGCAGGA TAACCTCCAA CAGCAACACG AAATGATGGG AATGGCCTCC 
CAGACTGGAA CGTCCCACTC CTCCCTGGCC ACCACTCCTC CCTCCACCGT TCAACCTATG 
CGGCAGAGCT CTGTTGCTCC GCCTCCCAGC CAGCGCGTCA AGTCCCAGAC TTCCCACCAG 
CTATCCGTCA GCGCGGCGGC CGCGCCCGCC GCCTCTGGTA AAAGCCGTCC ACAGAGCGGG 
AACCTCCTCC AGTCTCCAGA GTCTGGCTAC GGCGGCAGGC AGCACGGGCC CCGGCAGCTA 
TCGGTCAAAG ACAACACTGC CCCGGGTCTT CCCCACCAGC AGAGCTCTGT CAGGGAAAGC 
GGGAGTCCAC AAGGGACCAC CAGTCAGAGC ACTCAGCAGT CACCACAGCA GTCTCAACAG 
CATCTTTTAA AACCCACCAT GAGTAAACAG GGCTCCCAGT CACCTACCAC CCTCAATCCC 
CCGACCCCAG CGAACGAGCG AACAGTGGCC TGGGTCTCCA ACATGCCTCA CCTGTCGGCC 
GACATCGAAA GTTCACGAAT TGACCGAGAG GAGTTCAAGC TGAAGGAGTA TTCGAAAAGC 
ATGGATGAGT CGCGCATGGA CAGGGTCAAA GAGTACGAGG AGGAAATCAA CTCTTTGAAG 
GAGCGTCTGC TGATGTCTCA CAGAAAGCTG GAGGAGTACG AGAGGAGGCT CCTAGTGCAG 
GAGCAGCAGA CCAATAAGAT CCTCCTCCAG TACCAAAACC GTCTGGAGGA CAGCGAGAGG 
AGGCTACGAC AACAGCAAGT GGAGAAAGAC TCCCAAATTA AAGGAATAAT TAACAGGAAA 
GAAGACTCGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LOC103477581
Entrez Gene ID	103477581
Full Name	ras/Rap GTPase-activating protein SynGAP
Synonyms	syngap1
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC103477581 cDNA ORF clone, Poecilia reticulata(guppy)

Gene

Clones

gRNAs

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