ptrh1 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptrh1
Entrez Gene ID	103470709
Full Name	peptidyl-tRNA hydrolase 1 homolog
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008419340.2	XP_008417562.1	probable peptidyl-tRNA hydrolase

Pathways from BioSystems
Gene Ontology

The following ptrh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptrh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo201042	XM_008419340.2 Latest version!	Poecilia reticulata peptidyl-tRNA hydrolase 1 homolog (ptrh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptrh1 ( XM_008419340.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008417562.1 Poecilia reticulata peptidyl-tRNA hydrolase 1 homolog (ptrh1), mRNA.

Price & Availability↑

CloneID	OPo201042
Clone ID Related Accession (Same CDS sequence)	XM_008419340.2
Accession Version	XM_008419340.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	probable peptidyl-tRNA hydrolase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008419340.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACC GTTCGTTTTT GACTAAAGTC TGTCCGATTT TATCGCACTT CTTTAAGAAG 
CTGCTGAACT TTGTCACGAT GAGAAGACTT TTAACGAGAG TCATAAACCG AGTTTTGCTG 
GGAGAGCAGT TTAAAGCAAC GATGATAGGA GCTGAAGCGG AGATTCACAC AGAGGGTCGG 
AGAAAGTTGG TGGTGGGGCT CGGGAACCAA GGGATGGAGG GTACCAGACA CAGCGTGGGG 
ATGGCGGTGC TGGGAGCGCT GGCTGCCCGG CTCGGTGTGG CCGATTGTTG GCACGGCGAC 
CGTCATGTAT CCGGTGATGT CATCCTGACG GAGGTCCAGC AGGCCCGCGT GGTTCTGCTC 
CGTCCCCGGC TGCTGATGAA CGTCAACGGG GTGTCGGTGG CCAGAGCGGC TGGAAAATAC 
GGAGTCAAGC CTGAGGACAT CCTGCTGGTC CACGATGAGC TGGACAAACC TTTGGGGAAA 
ATCTCCATCA AACATGGAGG AAGCGCCAGA GGTCACAACG GAGTTCGCTC GTGTATCGAC 
TGTCTCCAGA CTGATGTGAT GCCCAGACTC CGGGTCGGGA TCGGCCGGCC GGCCGGGAAA 
ACCTCTGTGG AGCGCCACGT TCTGAGTCGG TTCACGACGG AGGAGCAGAA GGTTCTGGAA 
GCGGTTCTGG TCCAGAGCGT GGATCTGCTC ATCTCCCAGC TCTCCCAGCA GGACACGCAG 
TCTGCCTCCT CACCAGCAGG GGGCAGGCGA GCAGCGCAGC ACAGGAAGAA GGAGGAGGAC 
TCCACAGCTG TGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008417562.1
CDS	241..1038
Translation	MKNRSFLTKVCPILSHFFKKLLNFVTMRRLLTRVINRVLLGEQFKATMIGAEAEIHTEGRRKLVVGLGNQGMEGTRHSVGMAVLGALAARLGVADCWHGDRHVSGDVILTEVQQARVVLLRPRLLMNVNGVSVARAAGKYGVKPEDILLVHDELDKPLGKISIKHGGSARGHNGVRSCIDCLQTDVMPRLRVGIGRPAGKTSVERHVLSRFTTEEQKVLEAVLVQSVDLLISQLSQQDTQSASSPAGGRRAAQHRKKEEDSTAVI

Target ORF information:

RefSeq Version	XM_008419340.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata peptidyl-tRNA hydrolase 1 homolog (ptrh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008419340.2

ORF Insert Sequence:

ATGAAAAACC GTTCGTTTTT GACTAAAGTC TGTCCGATTT TATCGCACTT CTTTAAGAAG 
CTGCTGAACT TTGTCACGAT GAGAAGACTT TTAACGAGAG TCATAAACCG AGTTTTGCTG 
GGAGAGCAGT TTAAAGCAAC GATGATAGGA GCTGAAGCGG AGATTCACAC AGAGGGTCGG 
AGAAAGTTGG TGGTGGGGCT CGGGAACCAA GGGATGGAGG GTACCAGACA CAGCGTGGGG 
ATGGCGGTGC TGGGAGCGCT GGCTGCCCGG CTCGGTGTGG CCGATTGTTG GCACGGCGAC 
CGTCATGTAT CCGGTGATGT CATCCTGACG GAGGTCCAGC AGGCCCGCGT GGTTCTGCTC 
CGTCCCCGGC TGCTGATGAA CGTCAACGGG GTGTCGGTGG CCAGAGCGGC TGGAAAATAC 
GGAGTCAAGC CTGAGGACAT CCTGCTGGTC CACGATGAGC TGGACAAACC TTTGGGGAAA 
ATCTCCATCA AACATGGAGG AAGCGCCAGA GGTCACAACG GAGTTCGCTC GTGTATCGAC 
TGTCTCCAGA CTGATGTGAT GCCCAGACTC CGGGTCGGGA TCGGCCGGCC GGCCGGGAAA 
ACCTCTGTGG AGCGCCACGT TCTGAGTCGG TTCACGACGG AGGAGCAGAA GGTTCTGGAA 
GCGGTTCTGG TCCAGAGCGT GGATCTGCTC ATCTCCCAGC TCTCCCAGCA GGACACGCAG 
TCTGCCTCCT CACCAGCAGG GGGCAGGCGA GCAGCGCAGC ACAGGAAGAA GGAGGAGGAC 
TCCACAGCTG TGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.