mccc2 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	mccc2
Entrez Gene ID	103469965
Full Name	methylcrotonoyl-CoA carboxylase 2
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008418054.2	XP_008416276.1	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial

Pathways from BioSystems
Gene Ontology

The following mccc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mccc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo202627	XM_008418054.2 Latest version!	Poecilia reticulata methylcrotonoyl-CoA carboxylase 2 (mccc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mccc2 ( XM_008418054.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008416276.1 Poecilia reticulata methylcrotonoyl-CoA carboxylase 2 (mccc2), mRNA.

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CloneID	OPo202627
Clone ID Related Accession (Same CDS sequence)	XM_008418054.2
Accession Version	XM_008418054.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008418054.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTTAC AGTCCGTGAG GCCGTGGTTG CTTCGATGCA GGGTATTACC GCTGCCTTGT 
ACACGGACTT ACTACGCTGA CAGAGTCGCT CGGCTCGGCT CCCAACCTGA TAAACATTCC 
TCTGAATATC AGGAAAATTA TGAGCGAATG AAAGTTTTGG TAGATCAACT GAGGGACCGG 
ACAGAGAAAA TCAAGTTGGG TGGAGGAGAA AAAGCTAGAA CACTTCACAC ATCTCGAGGG 
AAGCTGTTGC CAAGAGAGCG CATAGATAGA CTACTGGATC CAGGGACTCC TTTTTTGGAA 
TTTTCCCAGT TTGCAGGATA TGAGTTGTAT GGAAAAGAGG ATGTTCCGGC CGGGGGGATG 
ATTACTGGGA TAGGACGGGT TTCAGGGGTG GAATGTGTTG TTGTTGCAAA TGATGCCACC 
GTAAAAGGGG GAACATACTA CCCAATTACA GTGAAAAAAC ACCTTCGTGC ACAAGAAATT 
GCTCAGCAGA ACCATTTGCC ATGCATTTAT TTGGTGGATT CTGGAGGAGC CAATCTTCCC 
AGACAGGCCG ATGTCTTTCC AGACCGCGAT CACTTTGGAC GCATTTTCTA CAACCAGGCC 
AGGCTGTCTT CAGAAGGAAT TGCACAGATT GCTGTCGTGA TGGGCTCGTG CACGGCTGGA 
GGAGCATATG TTCCAGCAAT GGCAGACGAG AGCATCATTG TGCAAAAGCA AGGAACAATT 
TTTCTCGGAG GACCTCCTCT GGTGAAAGCT GCCACTGGGG AAGAAGTATC TGCAGAAGAT 
CTTGGTGGTG CTGATCTGCA CTGCAAAAAA TCTGGCGTGA CAGACCACTA CGCTTTAGAT 
GATAACCACG CACTCCATTT AGCTCGGAAG ACGGTCCGAA GTCTCAACTA CAGGAAGAAC 
ATTGAGGTTA CTGTAGAACC CACCGAAGCC CCGCTTTTTC CCTCAGATGA GCTTTATGGG 
ATAGTTGGAG ATAACTTGAA ACGCAACTTT GATGTCAGAG AGGTTATTGC CAGAATTGTG 
GACGGCAGTA AATTTGATGA GTTCAAGGCA TTTTATGGAG ACACGCTTGT TACAGGCTTC 
TCAAGAATCT TTGGATACCC TGTTGGAATC ATTGGCAACA ATGGAGTCCT GTTTTCAGAG 
TCTGCAAAAA AGGGAACCCA TTTCATCGAA TTATGCTGCC AAAGAAACAT TCCTCTGATC 
TTCCTTCAAA ATATAACAGG GTTCATGGTG GGAAGAGAGT ATGAAGCAGG AGGTATTGCC 
AAAGATGGAG CTAAGATGGT CACTGCTGTT GCCTGTGCCA ATGTGCCAAA GATGACAGTT 
ATCATCGGAG GCTCCTATGG TGCAGGCAAT TACGGCATGT GTGGAAGAGC GTACAGCCCC 
CGATTCCTCT ACATGTGGCC CAATTCAAGA ATCTCTGTGA TGGGAGGTGA GCAGGCGGCC 
ACAGTCCTGG CCACCATCAC TAAAGATCAG AGGACACGTG AAGGAAAGGA GTTCACAGCT 
GAACAAGAGG CAGCCATGAA GGAACCAATT GTCCGTCGGT TTGAAGAGGA AGGCAGTCCA 
TATTACTCGA GTGCCAGATT GTGGGATGAT GGGATCATTG ACCCTGCAGA CACTCGTCTG 
GTTCTGGGAC TAAGCCTCAG CGCAGCGCTC AATGCACCAA CAAAGAAGAC CCACTTCGGA 
GTGTTCAGGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008416276.1
CDS	87..1781
Translation	MLLQSVRPWLLRCRVLPLPCTRTYYADRVARLGSQPDKHSSEYQENYERMKVLVDQLRDRTEKIKLGGGEKARTLHTSRGKLLPRERIDRLLDPGTPFLEFSQFAGYELYGKEDVPAGGMITGIGRVSGVECVVVANDATVKGGTYYPITVKKHLRAQEIAQQNHLPCIYLVDSGGANLPRQADVFPDRDHFGRIFYNQARLSSEGIAQIAVVMGSCTAGGAYVPAMADESIIVQKQGTIFLGGPPLVKAATGEEVSAEDLGGADLHCKKSGVTDHYALDDNHALHLARKTVRSLNYRKNIEVTVEPTEAPLFPSDELYGIVGDNLKRNFDVREVIARIVDGSKFDEFKAFYGDTLVTGFSRIFGYPVGIIGNNGVLFSESAKKGTHFIELCCQRNIPLIFLQNITGFMVGREYEAGGIAKDGAKMVTAVACANVPKMTVIIGGSYGAGNYGMCGRAYSPRFLYMWPNSRISVMGGEQAATVLATITKDQRTREGKEFTAEQEAAMKEPIVRRFEEEGSPYYSSARLWDDGIIDPADTRLVLGLSLSAALNAPTKKTHFGVFRM

Target ORF information:

RefSeq Version	XM_008418054.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata methylcrotonoyl-CoA carboxylase 2 (mccc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008418054.2

ORF Insert Sequence:

ATGCTTTTAC AGTCCGTGAG GCCGTGGTTG CTTCGATGCA GGGTATTACC GCTGCCTTGT 
ACACGGACTT ACTACGCTGA CAGAGTCGCT CGGCTCGGCT CCCAACCTGA TAAACATTCC 
TCTGAATATC AGGAAAATTA TGAGCGAATG AAAGTTTTGG TAGATCAACT GAGGGACCGG 
ACAGAGAAAA TCAAGTTGGG TGGAGGAGAA AAAGCTAGAA CACTTCACAC ATCTCGAGGG 
AAGCTGTTGC CAAGAGAGCG CATAGATAGA CTACTGGATC CAGGGACTCC TTTTTTGGAA 
TTTTCCCAGT TTGCAGGATA TGAGTTGTAT GGAAAAGAGG ATGTTCCGGC CGGGGGGATG 
ATTACTGGGA TAGGACGGGT TTCAGGGGTG GAATGTGTTG TTGTTGCAAA TGATGCCACC 
GTAAAAGGGG GAACATACTA CCCAATTACA GTGAAAAAAC ACCTTCGTGC ACAAGAAATT 
GCTCAGCAGA ACCATTTGCC ATGCATTTAT TTGGTGGATT CTGGAGGAGC CAATCTTCCC 
AGACAGGCCG ATGTCTTTCC AGACCGCGAT CACTTTGGAC GCATTTTCTA CAACCAGGCC 
AGGCTGTCTT CAGAAGGAAT TGCACAGATT GCTGTCGTGA TGGGCTCGTG CACGGCTGGA 
GGAGCATATG TTCCAGCAAT GGCAGACGAG AGCATCATTG TGCAAAAGCA AGGAACAATT 
TTTCTCGGAG GACCTCCTCT GGTGAAAGCT GCCACTGGGG AAGAAGTATC TGCAGAAGAT 
CTTGGTGGTG CTGATCTGCA CTGCAAAAAA TCTGGCGTGA CAGACCACTA CGCTTTAGAT 
GATAACCACG CACTCCATTT AGCTCGGAAG ACGGTCCGAA GTCTCAACTA CAGGAAGAAC 
ATTGAGGTTA CTGTAGAACC CACCGAAGCC CCGCTTTTTC CCTCAGATGA GCTTTATGGG 
ATAGTTGGAG ATAACTTGAA ACGCAACTTT GATGTCAGAG AGGTTATTGC CAGAATTGTG 
GACGGCAGTA AATTTGATGA GTTCAAGGCA TTTTATGGAG ACACGCTTGT TACAGGCTTC 
TCAAGAATCT TTGGATACCC TGTTGGAATC ATTGGCAACA ATGGAGTCCT GTTTTCAGAG 
TCTGCAAAAA AGGGAACCCA TTTCATCGAA TTATGCTGCC AAAGAAACAT TCCTCTGATC 
TTCCTTCAAA ATATAACAGG GTTCATGGTG GGAAGAGAGT ATGAAGCAGG AGGTATTGCC 
AAAGATGGAG CTAAGATGGT CACTGCTGTT GCCTGTGCCA ATGTGCCAAA GATGACAGTT 
ATCATCGGAG GCTCCTATGG TGCAGGCAAT TACGGCATGT GTGGAAGAGC GTACAGCCCC 
CGATTCCTCT ACATGTGGCC CAATTCAAGA ATCTCTGTGA TGGGAGGTGA GCAGGCGGCC 
ACAGTCCTGG CCACCATCAC TAAAGATCAG AGGACACGTG AAGGAAAGGA GTTCACAGCT 
GAACAAGAGG CAGCCATGAA GGAACCAATT GTCCGTCGGT TTGAAGAGGA AGGCAGTCCA 
TATTACTCGA GTGCCAGATT GTGGGATGAT GGGATCATTG ACCCTGCAGA CACTCGTCTG 
GTTCTGGGAC TAAGCCTCAG CGCAGCGCTC AATGCACCAA CAAAGAAGAC CCACTTCGGA 
GTGTTCAGGA TGTAA