bmp2k cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	bmp2k
Entrez Gene ID	103469814
Full Name	BMP2 inducible kinase
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008417765.2	XP_008415987.1	BMP-2-inducible protein kinase isoform X2
XM_008417764.2	XP_008415986.1	BMP-2-inducible protein kinase isoform X1

Pathways from BioSystems
Gene Ontology

The following bmp2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmp2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo201840	XM_008417764.2 Latest version!	Poecilia reticulata BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPo201841	XM_008417765.2 Latest version!	Poecilia reticulata BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmp2k ( XM_008417764.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008415986.1 Poecilia reticulata BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo201840
Clone ID Related Accession (Same CDS sequence)	XM_008417764.2
Accession Version	XM_008417764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	BMP-2-inducible protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024339.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008417764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCCAGAAT GCCAAAGTCC GAGAGCGGAG GGCTCGGAGG GGCGTCCGGT 
ACCAGCTCCA GCTCGGGGGT CAGCAGCTAC TTCGGGAAAG TGTTCGCGGT TGGTCGGTAC 
CAGGTCACCG TGGAGGAGCT CGTTGCAGAA GGGGGCTTCT CGGTGGTGTT CCTGGCTCGG 
ACCCACAGCG GCGTCCGCTG CGCTCTCAAA AGGATGTACG TGAACAACGT GCCGGACCTG 
AATATCTACA AGAGAGAGAT CACCATCATG AAGGAGCTGT CGGGCCACAA GAACATCGTC 
CGCTACCTGG ACTCGACAAT CAGCTTCGTT TCCGACAGCG TGTGGGAGGT TCTGATTCTC 
ATGGAGTACT GCAAAGCGGG TCAGGTGGTG AAGCAGATGA ATCAGCGGTT GAACGTGGGC 
TTCAGTGAGG CGGAGGTCCT CCACATCTTC TGCGATACCT GTGAAGCTGT GGCTCGCCTT 
CATCAATGCA AGACTCCCAT CATTCACAGA GACCTTAAGG TTGAAAACAT CCTCCTGAAC 
GACCAGGGAA ACTACGTCCT GTGTGACTTC GGCAGCTCCA CTCATAAGGT TCTGTTGCCA 
CTGAAAGACG GAGTGACGGC TGTGGAGGAT GAGATAAAGA AGTACACGAC CCTCTCATAT 
CGGGCCCCAG AGATGATTAA CTTGTACGCA GGGAAAGCCA TCACCACTAA GGCTGATATC 
TGGGCGCTCG GATGCTTGTT GTACAAGCTC TGTTTCTTCA CACTTCCATT TGGGGAGAGC 
CAGGTTGCCA TTTGTGACGG AACCTTTATC GTTCCAGATA ACTCCAAGTT CTCCACCAGG 
TTGCACTGCT TAATCAGATA TATGCTTGAG CCGGACCAGG AGAAGAGACC AGACATCTAC 
CAGGTGTCCT ACTTTGCCTT TAAACTAGCA GGAAGAGACT GCCCAGTCCC AAACCTCTCA 
AACTCTCCCA TCCCAACGTC GCTTCCTGAG CCTCTGACAG CCAGCGAGGT CGCTGCCAAG 
AAGAGCATGA CTAAAGCCAG GATAACCGAC TCCGTGGGTC CAACAGAAAC ATCCATCGCT 
CCCAGGCAGC GGCCGAAGGC GGCGAACAGC AACGTCCTGC CGCCGCTCAC CAACACCGTC 
ACTCCTGTCA AAATGGCCGT GCCTTCAGCG CCCGTCAGCA ACGGCCAGAA AGCTCCCACT 
CCTGGATCGG GACAGCCGTC CGTCCAGACG GCGCCCGGCA GCCAGCAGCA CCGCGTCCTG 
CAGCAGCTGC AGCCTGGAGA CCTGCGCCTC CAGCAGCTGC AGCAGCAGCA AGCCGTGCAG 
CAGCAGCAGC AGCTGCAATA CCTGCAGTAC CAACAGGCCG TGCAGCAGTC CCTGCAGCTC 
CAGCACCAGC AGGCTCTGCT GCTGCAGCAG CAGCAGCAGC AGCAGATGAT GATGCAGCCG 
ATGTATCAGC AGCAGGTGGC TCAGGCTCAG TACGCTGCCA TGCTGCACCA GTACCAGCAG 
GCTTTTGTCC AGCAGCAGCA GCAGCATCAT CATCATCATC ACCACCAGCA GCAGCATCAT 
CCCGCTCAGC AGCCTCTTCC CTATCTGTCT TCCCCTCTTG AGTTCCAGAT CCCTCTGGGT 
TCTTATAGTG CTGCCACGCC CACAGCTGGA CCCATCGCTG GACCATCACC TGTGGAAACC 
ACCTACACTA ACCCCAGCAG AAACCCCCTG CTGGCCACAG ACGGTATCAC GCCGACTTCC 
CAGAGCAGCA GCAGCAGCAG CAGCTTCAGC CACCCTCCCG ACATGTCGGG CTGGAACCCG 
TTCGGAGAAG ACAACTTCTC CAAACTGACC GAGGAGGAGC TGATCGACCG AGAGTTCGAC 
ATGCTCAGAG CAAAGAAGCC AGAAGAGCGA ACGGTCGGCG TGGAAACGGA CCCGGCGCCG 
GCTGCAGCTC TCCCGCCGGA GGATCTGTTC GGCTCGGTCC CCTTCGTGTC CAACGCAGGA 
ACTGGTGTGA TGCAGAGTGA GCCGCCTGTC GACGAGCTTC CCGCCAACGT CCAGGCCCCC 
GTCGAGGATC CTCAGCCGGT CAAGGAGCCA AAATCGGGAA AGAAGAGCAG CTGCAAACTC 
GCCGATGAGT CCGACAGCGA CTTCGAGTCC GACCCTCCAT CTCCAAAAAG CAGTGAAGAT 
GAAGAGCAGG AGGAAGAGGA GGCCTTGAAC AGCGAGCACG GTGACGACAC CGAGCCGGAA 
AATCTGGGTC AGAGGCCTCT ACTGATGGAC TCCGAGGAAG AGGAAGATGA TGATAAACAT 
AGCTCAGATT CGGACTGTGA TGTCAGCAAG GTCAAAGGCC GCTCAAAGAA ACCAGCTGCC 
GCTAAAGTAG CCAATCCCAC CGCTTCCAGG AGTCCTGGAG GGCGGTCTCA GGAGAGCCTC 
ATCACCCCTA CCGTGTCTTC CGGCAGAGCG GAAGGTGTGG ACGTGTTCGG CTCCATTCCT 
TTCGTTGGCG GAGCTTCGCC CGTCGGGCCC ACAGAGAGCA ACGATATCTT CACCAAAGCG 
CCTTTCCGGC AGGTCAGCCA GGAGGAGCTG GCTGCTGATG AGTTTGATGT TTTCACCAAA 
GCCCCGTTCA GCCGGAACGT GTCGAAGTCC GGAAGGAGCG GCAGTCAGAC GCCGCCCCTA 
TCCCCGGAAG GCACCGACAT CTTCGGCTTC TCTCCGTTCC AGCCGGGCCA CACAGAACCT 
CCGCCTACCT CCCGGAGCGC CGAAGACATC TTCCAGTCGC CGTTCGAGGA GCCGTCGAGT 
CTCCAGCAGA GACAGAAGCA GCGCAGCCTC CAGAAGTTGT CTTCCCGACA GAGAAAAACC 
AAGCAGGAGA CGACGGCGGG GGGCAGCAAC GGCAAGCGGC ACCACGGAAC CCCAACCGGA 
GGACGCAAAA CCAACAAGTC CAGCTACCGC ACTCCAGAGA AGGTCCGCAG GCACAAGAAG 
GTGGGCCGCC GAGATTCCCA GAGCAGCAAC GAGTTCCTGA GCACCTCCGA CTCCAAGGAG 
AACATCAGCG TGGACATCAG CATGGCCGAA GGGAAGGACA CCATGGCGCC TCTTCCGGTG 
GACGACGTTC TTCTAGACCC GTTCGGGGCC AAACCCTTCC ACCCTCAGTA CCCGGGCCTT 
GGCGACGGTA AACCCGACAT GAGCACCGCC AACGGGGGAA GGATCATAGA TGATTTCGGG 
GCGGTTCCCT TCACGGAACT GGTGATCCAC AGCGGGCCGC AGCAGCCGGT GGTGGCGCCT 
TCGCAGCCGC TGGACCTGGA CCCTTTTGGA GCGGCGCCGT TCCCCTCCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008415986.1
CDS	710..4006
Translation	MKKFSRMPKSESGGLGGASGTSSSSGVSSYFGKVFAVGRYQVTVEELVAEGGFSVVFLARTHSGVRCALKRMYVNNVPDLNIYKREITIMKELSGHKNIVRYLDSTISFVSDSVWEVLILMEYCKAGQVVKQMNQRLNVGFSEAEVLHIFCDTCEAVARLHQCKTPIIHRDLKVENILLNDQGNYVLCDFGSSTHKVLLPLKDGVTAVEDEIKKYTTLSYRAPEMINLYAGKAITTKADIWALGCLLYKLCFFTLPFGESQVAICDGTFIVPDNSKFSTRLHCLIRYMLEPDQEKRPDIYQVSYFAFKLAGRDCPVPNLSNSPIPTSLPEPLTASEVAAKKSMTKARITDSVGPTETSIAPRQRPKAANSNVLPPLTNTVTPVKMAVPSAPVSNGQKAPTPGSGQPSVQTAPGSQQHRVLQQLQPGDLRLQQLQQQQAVQQQQQLQYLQYQQAVQQSLQLQHQQALLLQQQQQQQMMMQPMYQQQVAQAQYAAMLHQYQQAFVQQQQQHHHHHHHQQQHHPAQQPLPYLSSPLEFQIPLGSYSAATPTAGPIAGPSPVETTYTNPSRNPLLATDGITPTSQSSSSSSSFSHPPDMSGWNPFGEDNFSKLTEEELIDREFDMLRAKKPEERTVGVETDPAPAAALPPEDLFGSVPFVSNAGTGVMQSEPPVDELPANVQAPVEDPQPVKEPKSGKKSSCKLADESDSDFESDPPSPKSSEDEEQEEEEALNSEHGDDTEPENLGQRPLLMDSEEEEDDDKHSSDSDCDVSKVKGRSKKPAAAKVANPTASRSPGGRSQESLITPTVSSGRAEGVDVFGSIPFVGGASPVGPTESNDIFTKAPFRQVSQEELAADEFDVFTKAPFSRNVSKSGRSGSQTPPLSPEGTDIFGFSPFQPGHTEPPPTSRSAEDIFQSPFEEPSSLQQRQKQRSLQKLSSRQRKTKQETTAGGSNGKRHHGTPTGGRKTNKSSYRTPEKVRRHKKVGRRDSQSSNEFLSTSDSKENISVDISMAEGKDTMAPLPVDDVLLDPFGAKPFHPQYPGLGDGKPDMSTANGGRIIDDFGAVPFTELVIHSGPQQPVVAPSQPLDLDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_008417764.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008417764.2

ORF Insert Sequence:

ATGAAGAAGT TTTCCAGAAT GCCAAAGTCC GAGAGCGGAG GGCTCGGAGG GGCGTCCGGT 
ACCAGCTCCA GCTCGGGGGT CAGCAGCTAC TTCGGGAAAG TGTTCGCGGT TGGTCGGTAC 
CAGGTCACCG TGGAGGAGCT CGTTGCAGAA GGGGGCTTCT CGGTGGTGTT CCTGGCTCGG 
ACCCACAGCG GCGTCCGCTG CGCTCTCAAA AGGATGTACG TGAACAACGT GCCGGACCTG 
AATATCTACA AGAGAGAGAT CACCATCATG AAGGAGCTGT CGGGCCACAA GAACATCGTC 
CGCTACCTGG ACTCGACAAT CAGCTTCGTT TCCGACAGCG TGTGGGAGGT TCTGATTCTC 
ATGGAGTACT GCAAAGCGGG TCAGGTGGTG AAGCAGATGA ATCAGCGGTT GAACGTGGGC 
TTCAGTGAGG CGGAGGTCCT CCACATCTTC TGCGATACCT GTGAAGCTGT GGCTCGCCTT 
CATCAATGCA AGACTCCCAT CATTCACAGA GACCTTAAGG TTGAAAACAT CCTCCTGAAC 
GACCAGGGAA ACTACGTCCT GTGTGACTTC GGCAGCTCCA CTCATAAGGT TCTGTTGCCA 
CTGAAAGACG GAGTGACGGC TGTGGAGGAT GAGATAAAGA AGTACACGAC CCTCTCATAT 
CGGGCCCCAG AGATGATTAA CTTGTACGCA GGGAAAGCCA TCACCACTAA GGCTGATATC 
TGGGCGCTCG GATGCTTGTT GTACAAGCTC TGTTTCTTCA CACTTCCATT TGGGGAGAGC 
CAGGTTGCCA TTTGTGACGG AACCTTTATC GTTCCAGATA ACTCCAAGTT CTCCACCAGG 
TTGCACTGCT TAATCAGATA TATGCTTGAG CCGGACCAGG AGAAGAGACC AGACATCTAC 
CAGGTGTCCT ACTTTGCCTT TAAACTAGCA GGAAGAGACT GCCCAGTCCC AAACCTCTCA 
AACTCTCCCA TCCCAACGTC GCTTCCTGAG CCTCTGACAG CCAGCGAGGT CGCTGCCAAG 
AAGAGCATGA CTAAAGCCAG GATAACCGAC TCCGTGGGTC CAACAGAAAC ATCCATCGCT 
CCCAGGCAGC GGCCGAAGGC GGCGAACAGC AACGTCCTGC CGCCGCTCAC CAACACCGTC 
ACTCCTGTCA AAATGGCCGT GCCTTCAGCG CCCGTCAGCA ACGGCCAGAA AGCTCCCACT 
CCTGGATCGG GACAGCCGTC CGTCCAGACG GCGCCCGGCA GCCAGCAGCA CCGCGTCCTG 
CAGCAGCTGC AGCCTGGAGA CCTGCGCCTC CAGCAGCTGC AGCAGCAGCA AGCCGTGCAG 
CAGCAGCAGC AGCTGCAATA CCTGCAGTAC CAACAGGCCG TGCAGCAGTC CCTGCAGCTC 
CAGCACCAGC AGGCTCTGCT GCTGCAGCAG CAGCAGCAGC AGCAGATGAT GATGCAGCCG 
ATGTATCAGC AGCAGGTGGC TCAGGCTCAG TACGCTGCCA TGCTGCACCA GTACCAGCAG 
GCTTTTGTCC AGCAGCAGCA GCAGCATCAT CATCATCATC ACCACCAGCA GCAGCATCAT 
CCCGCTCAGC AGCCTCTTCC CTATCTGTCT TCCCCTCTTG AGTTCCAGAT CCCTCTGGGT 
TCTTATAGTG CTGCCACGCC CACAGCTGGA CCCATCGCTG GACCATCACC TGTGGAAACC 
ACCTACACTA ACCCCAGCAG AAACCCCCTG CTGGCCACAG ACGGTATCAC GCCGACTTCC 
CAGAGCAGCA GCAGCAGCAG CAGCTTCAGC CACCCTCCCG ACATGTCGGG CTGGAACCCG 
TTCGGAGAAG ACAACTTCTC CAAACTGACC GAGGAGGAGC TGATCGACCG AGAGTTCGAC 
ATGCTCAGAG CAAAGAAGCC AGAAGAGCGA ACGGTCGGCG TGGAAACGGA CCCGGCGCCG 
GCTGCAGCTC TCCCGCCGGA GGATCTGTTC GGCTCGGTCC CCTTCGTGTC CAACGCAGGA 
ACTGGTGTGA TGCAGAGTGA GCCGCCTGTC GACGAGCTTC CCGCCAACGT CCAGGCCCCC 
GTCGAGGATC CTCAGCCGGT CAAGGAGCCA AAATCGGGAA AGAAGAGCAG CTGCAAACTC 
GCCGATGAGT CCGACAGCGA CTTCGAGTCC GACCCTCCAT CTCCAAAAAG CAGTGAAGAT 
GAAGAGCAGG AGGAAGAGGA GGCCTTGAAC AGCGAGCACG GTGACGACAC CGAGCCGGAA 
AATCTGGGTC AGAGGCCTCT ACTGATGGAC TCCGAGGAAG AGGAAGATGA TGATAAACAT 
AGCTCAGATT CGGACTGTGA TGTCAGCAAG GTCAAAGGCC GCTCAAAGAA ACCAGCTGCC 
GCTAAAGTAG CCAATCCCAC CGCTTCCAGG AGTCCTGGAG GGCGGTCTCA GGAGAGCCTC 
ATCACCCCTA CCGTGTCTTC CGGCAGAGCG GAAGGTGTGG ACGTGTTCGG CTCCATTCCT 
TTCGTTGGCG GAGCTTCGCC CGTCGGGCCC ACAGAGAGCA ACGATATCTT CACCAAAGCG 
CCTTTCCGGC AGGTCAGCCA GGAGGAGCTG GCTGCTGATG AGTTTGATGT TTTCACCAAA 
GCCCCGTTCA GCCGGAACGT GTCGAAGTCC GGAAGGAGCG GCAGTCAGAC GCCGCCCCTA 
TCCCCGGAAG GCACCGACAT CTTCGGCTTC TCTCCGTTCC AGCCGGGCCA CACAGAACCT 
CCGCCTACCT CCCGGAGCGC CGAAGACATC TTCCAGTCGC CGTTCGAGGA GCCGTCGAGT 
CTCCAGCAGA GACAGAAGCA GCGCAGCCTC CAGAAGTTGT CTTCCCGACA GAGAAAAACC 
AAGCAGGAGA CGACGGCGGG GGGCAGCAAC GGCAAGCGGC ACCACGGAAC CCCAACCGGA 
GGACGCAAAA CCAACAAGTC CAGCTACCGC ACTCCAGAGA AGGTCCGCAG GCACAAGAAG 
GTGGGCCGCC GAGATTCCCA GAGCAGCAAC GAGTTCCTGA GCACCTCCGA CTCCAAGGAG 
AACATCAGCG TGGACATCAG CATGGCCGAA GGGAAGGACA CCATGGCGCC TCTTCCGGTG 
GACGACGTTC TTCTAGACCC GTTCGGGGCC AAACCCTTCC ACCCTCAGTA CCCGGGCCTT 
GGCGACGGTA AACCCGACAT GAGCACCGCC AACGGGGGAA GGATCATAGA TGATTTCGGG 
GCGGTTCCCT TCACGGAACT GGTGATCCAC AGCGGGCCGC AGCAGCCGGT GGTGGCGCCT 
TCGCAGCCGC TGGACCTGGA CCCTTTTGGA GCGGCGCCGT TCCCCTCCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bmp2k ( XM_008417765.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008415987.1 Poecilia reticulata BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo201841
Clone ID Related Accession (Same CDS sequence)	XM_008417765.2
Accession Version	XM_008417765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	BMP-2-inducible protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024339.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008417765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCCAGAAT GCCAAAGTCC GAGAGCGGAG GGCTCGGAGG GGCGTCCGGT 
ACCAGCTCCA GCTCGGGGGT CAGCAGCTAC TTCGGGAAAG TGTTCGCGGT TGGTCGGTAC 
CAGGTCACCG TGGAGGAGCT CGTTGCAGAA GGGGGCTTCT CGGTGGTGTT CCTGGCTCGG 
ACCCACAGCG GCGTCCGCTG CGCTCTCAAA AGGATGTACG TGAACAACGT GCCGGACCTG 
AATATCTACA AGAGAGAGAT CACCATCATG AAGGAGCTGT CGGGCCACAA GAACATCGTC 
CGCTACCTGG ACTCGACAAT CAGCTTCGTT TCCGACAGCG TGTGGGAGGT TCTGATTCTC 
ATGGAGTACT GCAAAGCGGG TCAGGTGGTG AAGCAGATGA ATCAGCGGTT GAACGTGGGC 
TTCAGTGAGG CGGAGGTCCT CCACATCTTC TGCGATACCT GTGAAGCTGT GGCTCGCCTT 
CATCAATGCA AGACTCCCAT CATTCACAGA GACCTTAAGG TTGAAAACAT CCTCCTGAAC 
GACCAGGGAA ACTACGTCCT GTGTGACTTC GGCAGCTCCA CTCATAAGGT TCTGTTGCCA 
CTGAAAGACG GAGTGACGGC TGTGGAGGAT GAGATAAAGA AGTACACGAC CCTCTCATAT 
CGGGCCCCAG AGATGATTAA CTTGTACGCA GGGAAAGCCA TCACCACTAA GGCTGATATC 
TGGGCGCTCG GATGCTTGTT GTACAAGCTC TGTTTCTTCA CACTTCCATT TGGGGAGAGC 
CAGGTTGCCA TTTGTGACGG AACCTTTATC GTTCCAGATA ACTCCAAGTT CTCCACCAGG 
TTGCACTGCT TAATCAGATA TATGCTTGAG CCGGACCAGG AGAAGAGACC AGACATCTAC 
CAGGTGTCCT ACTTTGCCTT TAAACTAGCA GGAAGAGACT GCCCAGTCCC AAACCTCTCA 
AACTCTCCCA TCCCAACGTC GCTTCCTGAG CCTCTGACAG CCAGCGAGGT CGCTGCCAAG 
AAGAGCATGA CTAAAGCCAG GATAACCGAC TCCGTGGGTC CAACAGAAAC ATCCATCGCT 
CCCAGGCAGC GGCCGAAGGC GGCGAACAGC AACGTCCTGC CGCCGCTCAC CAACACCGTC 
ACTCCTGTCA AAATGGCCGT GCCTTCAGCG CCCGTCAGCA ACGGCCAGAA AGCTCCCACT 
CCTGGATCGG GACAGCCGTC CGTCCAGACG GCGCCCGGCA GCCAGCAGCA CCGCGTCCTG 
CAGCAGCTGC AGCCTGGAGA CCTGCGCCTC CAGCAGCTGC AGCAGCAGCA AGCCGTGCAG 
CAGCAGCAGC AGCTGCAATA CCTGCAGTAC CAACAGGCCG TGCAGCAGTC CCTGCAGCTC 
CAGCACCAGC AGGCTCTGCT GCTGCAGCAG CAGCAGCAGC AGCAGATGAT GATGCAGCCG 
ATGTATCAGC AGCAGGTGGC TCAGGCTCAG TACGCTGCCA TGCTGCACCA GTACCAGCAG 
GCTTTTGTCC AGCAGCAGCA GCAGCATCAT CATCATCATC ACCACCAGCA GCAGCATCAT 
CCCGCTCAGC AGCCTCTTCC CTATCTGTCT TCCCCTCTTG AGTTCCAGAT CCCTCTGGGT 
TCTTATAGTG CTGCCACGCC CACAGCTGGA CCCATCGCTG GACCATCACC TGTGGAAACC 
ACCTACACTA ACCCCAGAAA CCCCCTGCTG GCCACAGACG GTATCACGCC GACTTCCCAG 
AGCAGCAGCA GCAGCAGCAG CTTCAGCCAC CCTCCCGACA TGTCGGGCTG GAACCCGTTC 
GGAGAAGACA ACTTCTCCAA ACTGACCGAG GAGGAGCTGA TCGACCGAGA GTTCGACATG 
CTCAGAGCAA AGAAGCCAGA AGAGCGAACG GTCGGCGTGG AAACGGACCC GGCGCCGGCT 
GCAGCTCTCC CGCCGGAGGA TCTGTTCGGC TCGGTCCCCT TCGTGTCCAA CGCAGGAACT 
GGTGTGATGC AGAGTGAGCC GCCTGTCGAC GAGCTTCCCG CCAACGTCCA GGCCCCCGTC 
GAGGATCCTC AGCCGGTCAA GGAGCCAAAA TCGGGAAAGA AGAGCAGCTG CAAACTCGCC 
GATGAGTCCG ACAGCGACTT CGAGTCCGAC CCTCCATCTC CAAAAAGCAG TGAAGATGAA 
GAGCAGGAGG AAGAGGAGGC CTTGAACAGC GAGCACGGTG ACGACACCGA GCCGGAAAAT 
CTGGGTCAGA GGCCTCTACT GATGGACTCC GAGGAAGAGG AAGATGATGA TAAACATAGC 
TCAGATTCGG ACTGTGATGT CAGCAAGGTC AAAGGCCGCT CAAAGAAACC AGCTGCCGCT 
AAAGTAGCCA ATCCCACCGC TTCCAGGAGT CCTGGAGGGC GGTCTCAGGA GAGCCTCATC 
ACCCCTACCG TGTCTTCCGG CAGAGCGGAA GGTGTGGACG TGTTCGGCTC CATTCCTTTC 
GTTGGCGGAG CTTCGCCCGT CGGGCCCACA GAGAGCAACG ATATCTTCAC CAAAGCGCCT 
TTCCGGCAGG TCAGCCAGGA GGAGCTGGCT GCTGATGAGT TTGATGTTTT CACCAAAGCC 
CCGTTCAGCC GGAACGTGTC GAAGTCCGGA AGGAGCGGCA GTCAGACGCC GCCCCTATCC 
CCGGAAGGCA CCGACATCTT CGGCTTCTCT CCGTTCCAGC CGGGCCACAC AGAACCTCCG 
CCTACCTCCC GGAGCGCCGA AGACATCTTC CAGTCGCCGT TCGAGGAGCC GTCGAGTCTC 
CAGCAGAGAC AGAAGCAGCG CAGCCTCCAG AAGTTGTCTT CCCGACAGAG AAAAACCAAG 
CAGGAGACGA CGGCGGGGGG CAGCAACGGC AAGCGGCACC ACGGAACCCC AACCGGAGGA 
CGCAAAACCA ACAAGTCCAG CTACCGCACT CCAGAGAAGG TCCGCAGGCA CAAGAAGGTG 
GGCCGCCGAG ATTCCCAGAG CAGCAACGAG TTCCTGAGCA CCTCCGACTC CAAGGAGAAC 
ATCAGCGTGG ACATCAGCAT GGCCGAAGGG AAGGACACCA TGGCGCCTCT TCCGGTGGAC 
GACGTTCTTC TAGACCCGTT CGGGGCCAAA CCCTTCCACC CTCAGTACCC GGGCCTTGGC 
GACGGTAAAC CCGACATGAG CACCGCCAAC GGGGGAAGGA TCATAGATGA TTTCGGGGCG 
GTTCCCTTCA CGGAACTGGT GATCCACAGC GGGCCGCAGC AGCCGGTGGT GGCGCCTTCG 
CAGCCGCTGG ACCTGGACCC TTTTGGAGCG GCGCCGTTCC CCTCCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008415987.1
CDS	710..4003
Translation	MKKFSRMPKSESGGLGGASGTSSSSGVSSYFGKVFAVGRYQVTVEELVAEGGFSVVFLARTHSGVRCALKRMYVNNVPDLNIYKREITIMKELSGHKNIVRYLDSTISFVSDSVWEVLILMEYCKAGQVVKQMNQRLNVGFSEAEVLHIFCDTCEAVARLHQCKTPIIHRDLKVENILLNDQGNYVLCDFGSSTHKVLLPLKDGVTAVEDEIKKYTTLSYRAPEMINLYAGKAITTKADIWALGCLLYKLCFFTLPFGESQVAICDGTFIVPDNSKFSTRLHCLIRYMLEPDQEKRPDIYQVSYFAFKLAGRDCPVPNLSNSPIPTSLPEPLTASEVAAKKSMTKARITDSVGPTETSIAPRQRPKAANSNVLPPLTNTVTPVKMAVPSAPVSNGQKAPTPGSGQPSVQTAPGSQQHRVLQQLQPGDLRLQQLQQQQAVQQQQQLQYLQYQQAVQQSLQLQHQQALLLQQQQQQQMMMQPMYQQQVAQAQYAAMLHQYQQAFVQQQQQHHHHHHHQQQHHPAQQPLPYLSSPLEFQIPLGSYSAATPTAGPIAGPSPVETTYTNPRNPLLATDGITPTSQSSSSSSSFSHPPDMSGWNPFGEDNFSKLTEEELIDREFDMLRAKKPEERTVGVETDPAPAAALPPEDLFGSVPFVSNAGTGVMQSEPPVDELPANVQAPVEDPQPVKEPKSGKKSSCKLADESDSDFESDPPSPKSSEDEEQEEEEALNSEHGDDTEPENLGQRPLLMDSEEEEDDDKHSSDSDCDVSKVKGRSKKPAAAKVANPTASRSPGGRSQESLITPTVSSGRAEGVDVFGSIPFVGGASPVGPTESNDIFTKAPFRQVSQEELAADEFDVFTKAPFSRNVSKSGRSGSQTPPLSPEGTDIFGFSPFQPGHTEPPPTSRSAEDIFQSPFEEPSSLQQRQKQRSLQKLSSRQRKTKQETTAGGSNGKRHHGTPTGGRKTNKSSYRTPEKVRRHKKVGRRDSQSSNEFLSTSDSKENISVDISMAEGKDTMAPLPVDDVLLDPFGAKPFHPQYPGLGDGKPDMSTANGGRIIDDFGAVPFTELVIHSGPQQPVVAPSQPLDLDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_008417765.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008417765.2

ORF Insert Sequence:

ATGAAGAAGT TTTCCAGAAT GCCAAAGTCC GAGAGCGGAG GGCTCGGAGG GGCGTCCGGT 
ACCAGCTCCA GCTCGGGGGT CAGCAGCTAC TTCGGGAAAG TGTTCGCGGT TGGTCGGTAC 
CAGGTCACCG TGGAGGAGCT CGTTGCAGAA GGGGGCTTCT CGGTGGTGTT CCTGGCTCGG 
ACCCACAGCG GCGTCCGCTG CGCTCTCAAA AGGATGTACG TGAACAACGT GCCGGACCTG 
AATATCTACA AGAGAGAGAT CACCATCATG AAGGAGCTGT CGGGCCACAA GAACATCGTC 
CGCTACCTGG ACTCGACAAT CAGCTTCGTT TCCGACAGCG TGTGGGAGGT TCTGATTCTC 
ATGGAGTACT GCAAAGCGGG TCAGGTGGTG AAGCAGATGA ATCAGCGGTT GAACGTGGGC 
TTCAGTGAGG CGGAGGTCCT CCACATCTTC TGCGATACCT GTGAAGCTGT GGCTCGCCTT 
CATCAATGCA AGACTCCCAT CATTCACAGA GACCTTAAGG TTGAAAACAT CCTCCTGAAC 
GACCAGGGAA ACTACGTCCT GTGTGACTTC GGCAGCTCCA CTCATAAGGT TCTGTTGCCA 
CTGAAAGACG GAGTGACGGC TGTGGAGGAT GAGATAAAGA AGTACACGAC CCTCTCATAT 
CGGGCCCCAG AGATGATTAA CTTGTACGCA GGGAAAGCCA TCACCACTAA GGCTGATATC 
TGGGCGCTCG GATGCTTGTT GTACAAGCTC TGTTTCTTCA CACTTCCATT TGGGGAGAGC 
CAGGTTGCCA TTTGTGACGG AACCTTTATC GTTCCAGATA ACTCCAAGTT CTCCACCAGG 
TTGCACTGCT TAATCAGATA TATGCTTGAG CCGGACCAGG AGAAGAGACC AGACATCTAC 
CAGGTGTCCT ACTTTGCCTT TAAACTAGCA GGAAGAGACT GCCCAGTCCC AAACCTCTCA 
AACTCTCCCA TCCCAACGTC GCTTCCTGAG CCTCTGACAG CCAGCGAGGT CGCTGCCAAG 
AAGAGCATGA CTAAAGCCAG GATAACCGAC TCCGTGGGTC CAACAGAAAC ATCCATCGCT 
CCCAGGCAGC GGCCGAAGGC GGCGAACAGC AACGTCCTGC CGCCGCTCAC CAACACCGTC 
ACTCCTGTCA AAATGGCCGT GCCTTCAGCG CCCGTCAGCA ACGGCCAGAA AGCTCCCACT 
CCTGGATCGG GACAGCCGTC CGTCCAGACG GCGCCCGGCA GCCAGCAGCA CCGCGTCCTG 
CAGCAGCTGC AGCCTGGAGA CCTGCGCCTC CAGCAGCTGC AGCAGCAGCA AGCCGTGCAG 
CAGCAGCAGC AGCTGCAATA CCTGCAGTAC CAACAGGCCG TGCAGCAGTC CCTGCAGCTC 
CAGCACCAGC AGGCTCTGCT GCTGCAGCAG CAGCAGCAGC AGCAGATGAT GATGCAGCCG 
ATGTATCAGC AGCAGGTGGC TCAGGCTCAG TACGCTGCCA TGCTGCACCA GTACCAGCAG 
GCTTTTGTCC AGCAGCAGCA GCAGCATCAT CATCATCATC ACCACCAGCA GCAGCATCAT 
CCCGCTCAGC AGCCTCTTCC CTATCTGTCT TCCCCTCTTG AGTTCCAGAT CCCTCTGGGT 
TCTTATAGTG CTGCCACGCC CACAGCTGGA CCCATCGCTG GACCATCACC TGTGGAAACC 
ACCTACACTA ACCCCAGAAA CCCCCTGCTG GCCACAGACG GTATCACGCC GACTTCCCAG 
AGCAGCAGCA GCAGCAGCAG CTTCAGCCAC CCTCCCGACA TGTCGGGCTG GAACCCGTTC 
GGAGAAGACA ACTTCTCCAA ACTGACCGAG GAGGAGCTGA TCGACCGAGA GTTCGACATG 
CTCAGAGCAA AGAAGCCAGA AGAGCGAACG GTCGGCGTGG AAACGGACCC GGCGCCGGCT 
GCAGCTCTCC CGCCGGAGGA TCTGTTCGGC TCGGTCCCCT TCGTGTCCAA CGCAGGAACT 
GGTGTGATGC AGAGTGAGCC GCCTGTCGAC GAGCTTCCCG CCAACGTCCA GGCCCCCGTC 
GAGGATCCTC AGCCGGTCAA GGAGCCAAAA TCGGGAAAGA AGAGCAGCTG CAAACTCGCC 
GATGAGTCCG ACAGCGACTT CGAGTCCGAC CCTCCATCTC CAAAAAGCAG TGAAGATGAA 
GAGCAGGAGG AAGAGGAGGC CTTGAACAGC GAGCACGGTG ACGACACCGA GCCGGAAAAT 
CTGGGTCAGA GGCCTCTACT GATGGACTCC GAGGAAGAGG AAGATGATGA TAAACATAGC 
TCAGATTCGG ACTGTGATGT CAGCAAGGTC AAAGGCCGCT CAAAGAAACC AGCTGCCGCT 
AAAGTAGCCA ATCCCACCGC TTCCAGGAGT CCTGGAGGGC GGTCTCAGGA GAGCCTCATC 
ACCCCTACCG TGTCTTCCGG CAGAGCGGAA GGTGTGGACG TGTTCGGCTC CATTCCTTTC 
GTTGGCGGAG CTTCGCCCGT CGGGCCCACA GAGAGCAACG ATATCTTCAC CAAAGCGCCT 
TTCCGGCAGG TCAGCCAGGA GGAGCTGGCT GCTGATGAGT TTGATGTTTT CACCAAAGCC 
CCGTTCAGCC GGAACGTGTC GAAGTCCGGA AGGAGCGGCA GTCAGACGCC GCCCCTATCC 
CCGGAAGGCA CCGACATCTT CGGCTTCTCT CCGTTCCAGC CGGGCCACAC AGAACCTCCG 
CCTACCTCCC GGAGCGCCGA AGACATCTTC CAGTCGCCGT TCGAGGAGCC GTCGAGTCTC 
CAGCAGAGAC AGAAGCAGCG CAGCCTCCAG AAGTTGTCTT CCCGACAGAG AAAAACCAAG 
CAGGAGACGA CGGCGGGGGG CAGCAACGGC AAGCGGCACC ACGGAACCCC AACCGGAGGA 
CGCAAAACCA ACAAGTCCAG CTACCGCACT CCAGAGAAGG TCCGCAGGCA CAAGAAGGTG 
GGCCGCCGAG ATTCCCAGAG CAGCAACGAG TTCCTGAGCA CCTCCGACTC CAAGGAGAAC 
ATCAGCGTGG ACATCAGCAT GGCCGAAGGG AAGGACACCA TGGCGCCTCT TCCGGTGGAC 
GACGTTCTTC TAGACCCGTT CGGGGCCAAA CCCTTCCACC CTCAGTACCC GGGCCTTGGC 
GACGGTAAAC CCGACATGAG CACCGCCAAC GGGGGAAGGA TCATAGATGA TTTCGGGGCG 
GTTCCCTTCA CGGAACTGGT GATCCACAGC GGGCCGCAGC AGCCGGTGGT GGCGCCTTCG 
CAGCCGCTGG ACCTGGACCC TTTTGGAGCG GCGCCGTTCC CCTCCAAGCA GTAA